LOCUS       NZ_CP015142             4072 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain Ecol_732 plasmid pEC732_4, complete
            sequence.
ACCESSION   NZ_CP015142
VERSION     NZ_CP015142.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04621897
            Assembly: GCF_001617565.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Stoesser,N., Sheppard,A., Peirano,G., Sebra,R., Lynch,T., Anson,L.,
            Kasarskis,A., Motyl,M., Kazmierczak,K., Crook,D. and Pitout,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2016) Department of MIcrobiology (Research), John
            Radcliffe Hospital, University of Oxford, Headley Way, Oxford OX3
            9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.2.0
            Genome Coverage                   :: 1x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001617565.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 01:47:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,381
            CDSs (total)                      :: 5,268
            Genes (coding)                    :: 5,043
            CDSs (with protein)               :: 5,043
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 84 of 225
            Pseudo Genes (incomplete)         :: 167 of 225
            Pseudo Genes (internal stop)      :: 38 of 225
            Pseudo Genes (multiple problems)  :: 54 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP015142.1.
            Bacteria available from Johann Pitout, University of Calgary
            Laboratory Services, Calgary, Alberta, Canada, or Nicole Stoesser,
            Department of Microbiology, John Radcliffe Hospital, Headley Way,
            Oxford, UK.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ecol_732"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC732_4"
                     /geo_loc_name="Thailand: Bangkok"
                     /lat_lon="13.76 N 100.50 E"
                     /collection_date="2012"
                     /collected_by="Merck Study for Monitoring of Antimicrobial
                     Resistance Trends (SMART)"
     gene            complement(256..948)
                     /locus_tag="A4X18_RS27350"
                     /old_locus_tag="A4X18_27335"
                     /old_locus_tag="A5H92_RS27335"
     CDS             complement(256..948)
                     /locus_tag="A4X18_RS27350"
                     /old_locus_tag="A4X18_27335"
                     /old_locus_tag="A5H92_RS27335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281403.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1480..1734)
                     /gene="mobC"
                     /locus_tag="A4X18_RS27355"
                     /old_locus_tag="A4X18_27340"
                     /old_locus_tag="A5H92_RS27340"
     CDS             complement(1480..1734)
                     /gene="mobC"
                     /locus_tag="A4X18_RS27355"
                     /old_locus_tag="A4X18_27340"
                     /old_locus_tag="A5H92_RS27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            1913..4072
                     /gene="mobQ"
                     /locus_tag="A4X18_RS27360"
                     /old_locus_tag="A4X18_27345"
                     /old_locus_tag="A5H92_RS27345"
     CDS             1913..4072
                     /gene="mobQ"
                     /locus_tag="A4X18_RS27360"
                     /old_locus_tag="A4X18_27345"
                     /old_locus_tag="A5H92_RS27345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029353.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     Typing          complement(1736..1912)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1913..2271
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          complement(257..948)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1827..4069
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.332"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtgaacaa tattttgttt tgtttaacat cgtgttatgt tcgtcgatgt tcttctatgt
       61 tcatcgacag ggatgaatgc aagaagcaca cacataatgc aagcatagtt ttgtttttcc
      121 gcccccaaaa attgggggtt cttttttcgc tgcattagag cgcttttgac gcaatttttc
      181 acttcaatag taccttcgca ctactaacac aaaaaaaacg cagcagggag cgttttaggt
      241 gccttaaaat cgattttaga tcgtcatctt gatctgtttg tcttccttga agcgaaaatg
      301 cagggctaca accgtgcgcc cctttttgat cgtctcaacc gttacagcca ggtcgctttt
      361 tttgttcagt tcgtcaacac agggttttat tagtatttta tttaagtctc tgaattgttt
      421 gtatttattt tcaatcccta acattgaacg aaagtcatta agagcaataa ttctctctcc
      481 cgtactgcgg aattgctgga ggaactcgta tattcttata ctgtaactac ttgataaacc
      541 tgatatgttt tttataacaa cgcgcgtgaa ttgccccttc agttgcgtca gataaggcat
      601 taccgcgtca gaaaaggtta ttcgagcctt ggcctcacct ttagcgtact gtgcccgata
      661 ctggatccag cggaactcct cacgcttctc gtcattcttg agaatgatcg acctgtccca
      721 taaacgatcg atcgcttcct gtaactgaac ctcggcattt ttccgcccca tgtcagggaa
      781 tgaatcaaaa tattccgcag cggtaatggt cattgtcttt tgcaattcag catcagcacc
      841 agatttcaat cgactgatac acagcaacag aaaacgttgt tcctgtaggg tcaacttgta
      901 gctggcctca accaaagcat tcgacttgta ggcaatcaag tccgacatac aaagatcccg
      961 ttcaaatgtg gtgatgcttt aatgtgcccc acaaataaga aatgtggaat attttttttt
     1021 atatcctcac atttctgtga ataacacctt aaattcctcc acctttacct taaattcctc
     1081 cacctaaaca ccttaaattc ctccaccttt accttaaatt cctccacata tacctctata
     1141 acatattgat tttattaatg aattttgacc ctaaaacaag tattaaaaac aagatcttaa
     1201 aaacaagatc taaaaactta acggcgagcc tgtggataac ttggggcgct gaaaacaaaa
     1261 aaccaataaa aacaatgtgt tgcaagaaag cagaattgca agttccgaat actccccttc
     1321 caaccctgtg gaaatccagt attcatgcgg gttcagcgca tatcacctac cgaaaaaccg
     1381 tgcgtatgtg ctcaacctgg cgacggattc acttccgctt cctcgcccac tcgctcgcta
     1441 acgctcggtc gctggggctg cggcgagcgg acgatacaat cactgtcctg gcagcgcctc
     1501 cagctcgtta atcagcggca gtatgcgcgt cacatcagcc tgtcgtgtca cctctgcctt
     1561 caggaactgg agtaaccagg cacgcaactg cacctcctcc ggctttctga cctttgccag
     1621 caatgctgca cccacaacaa ccttctgcgc tgtgtccagg ctccggctct tcgccttcag
     1681 gcgctgtaat ctcgcctcag catcagcaat cttctgttcg agcgttctgc tcatgatgta
     1741 actccgtgcg cgatgaaaaa tcgcatttta gcgcgtcact ggtagtttaa aaactgagct
     1801 ggcataatgc gcgacaaatc acaaagtgcg cacttataca atctccactc cgtttcgatt
     1861 gtgtgcgccc tgcggggctg aaagaaaact acaaaaaggc attacggcag aaatggcgat
     1921 ttatcatctc agcatgaaaa ttatctccag aagcagcggc tacagcgccg tagcttccgc
     1981 tgcctatcgt tccggttcac tgatgcttga tgagcgtacc ggactgactc acgactacac
     2041 ccgcaaaagc ggtgtggctg aagctgtgat tctgaccccc gcgacagccc ctgcctggtg
     2101 tacgaaccgt gccgaactct ggaatgccgt tgagaaggcc gaatgccgga agaactcaca
     2161 acttgcgcgg gaaattgaac tcgctatccc ccgtgaactg ccgcaggatg ccgcacggga
     2221 aaccgttctc gcctttgtca gagaaaactt tgtcagtcag ggcatgattg ctgatgtggc
     2281 gttccatcac atggataaaa caaatcccca tgcgcacatc atgctgacca cgagggctgt
     2341 cggacctgct gggttcggcg gaaaggtcag ggactggaac gaccggacac atgccgagac
     2401 gtggcgtgca tcatgggctg accatgcaaa cagagcactg gcgaacgccg gttatcagga
     2461 agagatagac catcgttcat acgagcgaca ggggcttgag aaaacgcctg gtattcacct
     2521 cggaaagtct gcctgtgcga tggaaacacg cggtatcgaa acagaacgcg gcgaacagaa
     2581 tcgtctgatt aacaggctta acctggaaat acagatttcc cgcactgaac tcaggaacag
     2641 cgggctgaca gagcctgccc gccgtgtggc tgaccttctg aatatcgtgc ttcctgacaa
     2701 cgcaacttcg tacacactac gtgacatcat cgaagcgttg ccgaaggaca gtaatgcggc
     2761 atggaaactc accagtaatg ccatgtcgat gatggcagat atgcaggcga cgcgcagacg
     2821 ctgggaagag ctgaacaccg aacgaaagga agcgatgagc catgctgcca gcctgaaaaa
     2881 atcatcgccg ttttcgtccg gtttttcccg tattcccctg atgcaatggg cggcaccgga
     2941 ataccgtagc gaacagggaa aaatccagtc gctgagccag caaatggaaa aattgcgaca
     3001 gcattaccgg aaggtggaga aacaggacat tcccgcgagt cagacggcat ttgaaaaaca
     3061 gtggcagcag tggggggcat ccggccttgc agctctcaga gtccagttgc ttaaacgtga
     3121 agaggaattg cgccggaagg agcaggagga tacagaacgt agtcgcgcgg agcgacgagc
     3181 ggaacagctc aggaaaaatg acaatgccgt tatggattca ggggtactga gcgcagtcgt
     3241 tacggggtat ggcgaggccc ccatgcctgg taacggggaa atgacctgtt accttctgct
     3301 tcacaaccgg aacggagaat acacgctctg ggggaacgag ctggagaaat accgcaccag
     3361 aatctttgag cgtgttgatt taatgcgtga caggagtggt tatatctgtg accgaagcga
     3421 aatcggtcaa catccaccac tgcaacgtgt ttattcctct gcgacctttg agcaacttct
     3481 gacgcaggtt tgccagacat ggccgcaata tacccgcaat ctccgccagc cgaagacatg
     3541 gccggagtct ttctgtcttg gagaagaccg ccagccagcc atgccatcac tggcagcaag
     3601 gaaggtggat tttacgcagg gacgtctgct accgacgctg atgccggtaa tgagttctgt
     3661 cgaccgggaa acccgccagc ttcagctttt gctggtcatg ggcgtggatg actctctggg
     3721 cggtgttgtc cggcttaatg gtacgctgta tcccgcgttt gccgttccct cagccgataa
     3781 cagccagctg gtcatcagtg cactgaccga taaggggtta cggtatgcag ggtatggtgt
     3841 tgccgtaaat cacgatgcag acagccacat atcacctgcg ccggaactta tggagtttca
     3901 tctgaaaacc cgtgaggcac cgctttttgc tgccgtcaac acaccagaaa aacagccgga
     3961 tcatctgttc cgctctctgg gtttcaatcg tacctgggat gaatggcggc gcgaggaaga
     4021 tgcaagaaca cacaccaccg agaggagaca tgatcgcggt tggtcacagt ga
//
