LOCUS       NZ_CP014660             9323 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Salmonella enterica subsp. enterica serovar Anatum str.
            USDA-ARS-USMARC-1765 plasmid pSAN1-06-0624, complete sequence.
ACCESSION   NZ_CP014660
VERSION     NZ_CP014660.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02716855
            Assembly: GCF_001620135.2
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Anatum str.
            USDA-ARS-USMARC-1765
  ORGANISM  Salmonella enterica subsp. enterica serovar Anatum str. USDA-ARS...
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Nguyen,S.V., Harhay,D.M., Bono,J.L., Smith,T.P., Fields,P.I.,
            Dinsmore,B.A., Santovenia,M., Kelley,C.M., Wang,R., Bosilevac,J.M.
            and Harhay,G.P.
  TITLE     Complete and Closed Genome Sequences of 10 Salmonella enterica
            subsp. enterica Serovar Anatum Isolates from Human and Bovine
            Sources
  JOURNAL   Genome Announc 4 (3), e00447-16 (2016)
   PUBMED   27257192
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9323)
  AUTHORS   Nguyen,S.V., Harhay,D.M., Bono,J.L., Smith,T.P.L., Fields,P.I.,
            Dinsmore,B.A., Santovinia,M., Kelley,C.M., Wang,R., Bosilevac,J.M.
            and Harhay,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2016) Meat Safety & Quality Research Unit,
            USDA-ARS U.S. Meat Animal Research Center, 844 Rd 313, Clay Center,
            NE 68933, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera v. 7.0
            Genome Coverage                   :: 261.99
            Sequencing Technology             :: PacBio RS II C3 P5
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 01:41:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,898
            CDSs (total)                      :: 4,785
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 50 of 118
            Pseudo Genes (incomplete)         :: 88 of 118
            Pseudo Genes (internal stop)      :: 17 of 118
            Pseudo Genes (multiple problems)  :: 33 of 118
            Pseudo Genes (short protein)      :: 1 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP014660.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9323
                     /organism="Salmonella enterica subsp. enterica serovar
                     Anatum str. USDA-ARS-USMARC-1765"
                     /mol_type="genomic DNA"
                     /strain="USDA-ARS-USMARC-1765"
                     /serovar="Anatum"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /sub_species="enterica"
                     /db_xref="taxon:1454593"
                     /plasmid="pSAN1-06-0624"
                     /geo_loc_name="USA: BIFSCo Region 5"
                     /collection_date="2006-07"
                     /collected_by="CDC"
                     /note="Bacteria and source DNA available from USDA-ARS U.S.
                     Meat Animal Research Center."
     gene            1..951
                     /locus_tag="AW58_RS25450"
                     /old_locus_tag="AW58_24280"
     CDS             1..951
                     /locus_tag="AW58_RS25450"
                     /old_locus_tag="AW58_24280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946646.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001293213.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
                     /name="replication initiation protein"
     gene            959..1372
                     /locus_tag="AW58_RS25455"
                     /old_locus_tag="AW58_24285"
     CDS             959..1372
                     /locus_tag="AW58_RS25455"
                     /old_locus_tag="AW58_24285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001229901.1"
                     /translation="MALVSISEASRLTNKSRTTVHRYISKGKLSICTDENGVKKIDTSE
                     LLRVFGAFKAVHHEQIDNVTDEQHVTPNRTGETSKTKQLEHEIEHLRQLVTAQQSHIDS
                     LKQAMLLIESKLPTTREPVTPPVGKKSWQFWKK"
                     /name="hypothetical protein"
     gene            1679..2647
                     /locus_tag="AW58_RS25460"
                     /old_locus_tag="AW58_24290"
     CDS             1679..2647
                     /locus_tag="AW58_RS25460"
                     /old_locus_tag="AW58_24290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="WP_012904651.1"
                     /translation="MKDQITHPPDNTDHSVAKQKFRITNWSTYNKALINRGSLTFWLDD
                     EAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFALMNVPLR
                     CPDYTSVSKRAKSVNVSFKTSTRGEIAHLVIDSTGLKVFGEGEWKVRKHGKERRRIWRK
                     LHLAVDSNTHEVVCADLSLNNVTDSEAFPGLIRQTHRKIRAAAADGAYDTRLCHDELRR
                     KKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETAMYRVK
                     QLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
                     /name="IS5 family transposase"
     gene            complement(2856..3395)
                     /locus_tag="AW58_RS25465"
                     /old_locus_tag="AW58_25900"
                     /pseudo=""
     CDS             complement(2856..3395)
                     /locus_tag="AW58_RS25465"
                     /old_locus_tag="AW58_25900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012710160.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(3382..4041)
                     /gene="tetR(C)"
                     /locus_tag="AW58_RS25470"
                     /old_locus_tag="AW58_24295"
     CDS             complement(3382..4041)
                     /gene="tetR(C)"
                     /locus_tag="AW58_RS25470"
                     /old_locus_tag="AW58_24295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(C)"
                     /protein_id="WP_001038045.1"
                     /translation="MNKLQREAVIRTALELLNDVGMEGLTTRRLAERLGVQQPALYWHF
                     KNKRALLDALAEAMLTINHTHSTPRDDDDWRSFLKGNACSFRRALLAYRDGARIHAGTR
                     PAAPQMEKADAQLRFLCDAGFSAGDATYALMAISYFTVGAVLEQQASEADAEERGEDQL
                     TTSASTMPARLQSAMKIVYEGGPDAAFERGLALIIGGLEKMRLTTNDIEVLKNVDE"
                     /name="tetR(C)"
     gene            4134..5324
                     /gene="tet(C)"
                     /locus_tag="AW58_RS25475"
                     /old_locus_tag="AW58_24300"
     ARG             4135..5321
                     /name="tet(C)"
                     /gene="tet(C)"
                     /gene_name="tetracycline efflux MFS transporter Tet(C)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            5228..5566
                     /locus_tag="AW58_RS25480"
                     /old_locus_tag="AW58_25905"
     CDS             5228..5566
                     /locus_tag="AW58_RS25480"
                     /old_locus_tag="AW58_25905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005291943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002310911.1"
                     /translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFTT
                     PRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRISGSVGSWPRVRMIVLLSLRTRL
                     GWRGCLTG"
                     /name="hypothetical protein"
     gene            5774..>6070
                     /locus_tag="AW58_RS25490"
                     /old_locus_tag="AW58_24310"
                     /pseudo=""
     CDS             5774..>6070
                     /locus_tag="AW58_RS25490"
                     /old_locus_tag="AW58_24310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(6387..6971)
                     /locus_tag="AW58_RS25495"
                     /old_locus_tag="AW58_24315"
     CDS             complement(6387..6971)
                     /locus_tag="AW58_RS25495"
                     /old_locus_tag="AW58_24315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_010891053.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQAASSQLQQQSETLSR
                     VIRELDSASGNMTDTVRKSVSSALTQVEKELKQAGLAQQKPATEALNQAADTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVLASCVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRTTYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            complement(6984..8099)
                     /gene="mobA"
                     /locus_tag="AW58_RS25500"
                     /old_locus_tag="AW58_24320"
     CDS             complement(6984..8099)
                     /gene="mobA"
                     /locus_tag="AW58_RS25500"
                     /old_locus_tag="AW58_24320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_016946648.1"
                     /translation="MASYHLSVKTGGKGSASPHADYIAREGKYAREKDSDLEHKESGNM
                     PAWAAHKPSEFWKAADTSERANGCTYREIEIALPRELKPEQRLELVRDFVQQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERINDGIHRDPEQYFKRANTKEPERGGAKKARFG
                     ETPTERKEYLVAQRERWADLQNKHLERYQHADRVDSRSLKAQGIDREPERHLGAGQVQR
                     FDTEQLQAILERREAERQVQQSRDERDSVIDVTTSLREALSERDTLTLKQELKSEPEQE
                     SHSGRTFDFEKEPDKLNALVSDAMKDIQEEIDLQSLVNDAMAEFQGIHQEMERQRERER
                     LAEKQRQQEKERQRLAEQIRQKPDKGWSFSR"
                     /name="mobA"
     gene            8263..8730
                     /gene="traD"
                     /locus_tag="AW58_RS25505"
                     /old_locus_tag="AW58_24325"
     CDS             8263..8730
                     /gene="traD"
                     /locus_tag="AW58_RS25505"
                     /old_locus_tag="AW58_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_016946647.1"
                     /translation="MNKSEKQEQWAAERVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSKTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSTT"
                     /name="traD"
     Typing          776..951
                     /name="ColE10"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(6988..8099)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.07"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtctgaat tagttgtttt caaagcaaat gaactagcga ttagtcgcta tgacttaacg
       61 gagcatgaaa ccaagctaat tttatgctgt gtggcactac tcaaccccac gattgaaaac
      121 cctacaagga aagaacggac ggtatcgttc acttataacc aatacgctca gatgatgaac
      181 atcagtaggg aaaatgctta tggtgtatta gctaaagcaa ccagagagct gatgacgaga
      241 actgtggaaa tcaggaatcc tttggttaaa ggctttgaga ttttccagtg gacaaactat
      301 gccaagttct caagcgaaaa attagaatta gtttttagtg aagagatatt gccttatctt
      361 ttccagttaa aaaaattcat aaaatataat ctggaacatg ttaagtcttt tgaaaacaaa
      421 tactctatga ggatttatga gtggttatta aaagaactaa cacaaaagaa aactcacaag
      481 gcaaatatag agattagcct tgatgaattt aagttcatgt taatgcttga aaataactac
      541 catgagttta aaaggcttaa ccaatgggtt ttgaaaccaa taagtaaaga tttaaacact
      601 tacagcaata tgaaattggt ggttgataag cgaggccgcc cgactgatac gttgattttc
      661 caagttgaac tagatagaca aatggatctc gtaaccgaac ttgagaacaa ccagataaaa
      721 atgaatggtg acaaaatacc aacaaccatt acatcagatt cctacctaca taacggacta
      781 agaaaaacac tacacgatgc tttaactgca aaaattcagc tcaccagttt tgaggcaaaa
      841 tttttgagtg acatgcaaag taagtatgat ctcaatggtt cgttctcatg gctcacgcaa
      901 aaacaacgaa ccacactaga gaacatactg gctaaatacg gaaggatctg aggttcttat
      961 ggctcttgta tctatcagtg aagcatcaag actaacaaac aaaagtagaa caactgttca
     1021 ccgttacata tcaaagggaa aactgtccat atgcacagat gaaaacggtg taaaaaagat
     1081 agatacatca gagcttttac gagtttttgg tgcattcaaa gctgttcacc atgaacagat
     1141 cgacaatgta acagatgaac agcatgtaac acctaataga acaggtgaaa ccagtaaaac
     1201 aaagcaacta gaacatgaaa ttgaacacct gagacaactt gttacagctc aacagtcaca
     1261 catagacagc ctgaaacagg cgatgctgct tatcgaatca aagctgccga caacacggga
     1321 gccagtgacg cctcccgtgg ggaaaaaatc atggcaattc tggaagaaat agcgctttca
     1381 gccggcaaac cggctgaagc cggatctgcg attctgataa caaactagca acaccagaac
     1441 agcccgtttg cgggcagcaa aacccgtact tttggacgtt ccggcggttt tttgtggcga
     1501 gtggtgttcg ggcggtgcgc gcaagatcca ttatgttaaa cgggcgagtt tacatctcaa
     1561 aaccgcccgc ttaacaccat cagaaatcct cagcgcgatt ttaagcacca accccccccc
     1621 gtaacaccca aatccatact gaaagtggct ttgttgaata aatcgaactt ttgctgagtt
     1681 gaaggatcag atcacgcatc ctcccgacaa cacagaccat tccgtggcaa agcaaaagtt
     1741 cagaatcacc aactggtcca cctacaacaa agctctcatc aaccgtggct ccctcacttt
     1801 ctggctggat gatgaggcga ttcaggcctg gtatgagtcg gcaacacctt catcacgagg
     1861 aaggccccag cgctattctg atctcgccat caccaccgtt ctggtgatta aacgcgtatt
     1921 ccggctgacc ctgcgggctg cgcagggttt tattgattcc atttttgccc tgatgaacgt
     1981 tccgttgcgc tgcccggatt acaccagtgt cagtaagcgg gcaaagtcgg ttaatgtcag
     2041 tttcaaaacg tccacccggg gtgaaatcgc acacctggtg attgattcca ccgggctgaa
     2101 ggtctttggt gaaggcgaat ggaaagtcag aaagcacggc aaagagcgcc gtcgtatctg
     2161 gcgaaagttg catcttgctg ttgacagcaa cacacatgaa gttgtctgtg cagacctgtc
     2221 gctgaataac gtcacggact cagaagcctt cccgggcctt atccggcaga ctcacagaaa
     2281 aatcagggca gccgcggcag acggggctta cgatacccgg ctctgtcacg atgaactgcg
     2341 ccgcaaaaaa atcagcgcgc ttattcctcc ccgaaaaggt gcgggttact ggcccggtga
     2401 atatgcagac cgtaaccgtg cagtggctaa tcagcgaatg accgggagta atgcgcggtg
     2461 gaaatggaca acagattaca accgtcgctc gatagcggaa acggcgatgt accgggtaaa
     2521 acagctgttc gggggttcac tgacgctgcg tgactacgat ggtcaggttg cggaggctat
     2581 ggccctggta cgagcgctga acaaaatgac gaaagcaggt atgcctgaaa gcgtgcgtat
     2641 tgcctgaaaa cacaacccgc tacgggggag acttacccga aatctgattt attcaacaaa
     2701 gccgttcctg acatccatcg gcaccatagt tctgcttatc cggcacgcgc gcgtcagggg
     2761 tgtggtgaac tacaaagcag acccgttgag gttatcagtt cgatgcacaa tcagcagcgc
     2821 ataaaatatg cacaagaaca ggagcaccct tcgcattaag ctgtggtggt aacaagtagt
     2881 gccgggctac catcagcgag catgatgcgc tcccacagca ttcgccttgg cagtatggaa
     2941 gttcctcgct ccagttcggg ccggtatcca cctcgagtga ccccagccgc ccctcatgcc
     3001 aaccgagccc attaagcgca gagtcggccg caactatctc ggggacgcat aaacacgcag
     3061 cgatttacca aggagttcgg ctaggtcaat ccgcaattcc ctttgcctgg ccggagatgc
     3121 cgcgagcaaa caatgtgtcc ggtcgctacc agatggactt tgtgggcgga ggattgcacc
     3181 agacgcttca gcgcagcgtc gtacgatgaa tcggaaggcc cggtaagcaa ttgaagaacc
     3241 gctgaccacc tcccatccga caatgcaagg gtgccccgct cccagcgcga cacggttgcc
     3301 tgatccacac ccattaattc agctaggtga ctttgcttca tatgacgaag caaccgcgcc
     3361 ctccgcacct gccgccccct gtcattcgtc aacattcttc agcacctcaa tgtcgttcgt
     3421 agtgagcctc attttttcaa gaccgccgat gatgagagcc aggcctcgct cgaatgccgc
     3481 gtccggaccg ccttcgtaga cgattttcat cgcgctctgt aggcgcgccg gcatcgtaga
     3541 cgctgaggtg gtcaactgat cttcgccccg ctcctcggcg tctgcctcgc tagcttgctg
     3601 ctcaagaaca gcgccgacgg tgaagtagct gattgccatc aacgcatagg tcgcgtcacc
     3661 tgccgaaaag ccagcatcgc aaaggaagcg aagctgcgcg tcggcttttt ccatctgcgg
     3721 cgcggctggc cgcgtcccgg catgaatacg cgcgccatcg cgataagcga gcaacgcccg
     3781 tcgaaaactg catgcattgc ccttcaggaa cgaacgccag tcgtcgtcat cccttggcgt
     3841 cgaatgcgtg tgatttatcg tcagcatggc ttcggcaagt gcgtcgagca acgcacgctt
     3901 gttcttgaaa tgccagtaga gcgctggctg ttgcaccccg aggcgctcag ccagtcggcg
     3961 cgtcgttaga ccttccatgc ccacgtcgtt aagcagttcg agcgcggttc ggatcacggc
     4021 ctcgcgttgg agcttgttca ttcgcgaatt ctcatgtttg acagcttatc atcgataagc
     4081 tttaatgcgg tagtttatca cagttaaatt gctaacgcag tcaggcaccg tgtatgaaat
     4141 ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct ggatgctgta ggcataggct
     4201 tggttatgcc ggtactgccg ggcctcttgc gggatatcgt ccattccgac agcatcgcca
     4261 gtcactatgg cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc
     4321 tcggagcact gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag
     4381 ccactatcga ctacgcgatc atggcgacca cacccgtcct gtggatcctc tacgccggac
     4441 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
     4501 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
     4561 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
     4621 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg
     4681 agtcgcataa gggagagcgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct
     4741 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc
     4801 aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct
     4861 ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc
     4921 aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg
     4981 gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg
     5041 ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca
     5101 tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc
     5161 ttaccagcct aacttcgatc attggaccgc tgatcttcac ggcgatttat gccgcctcgg
     5221 cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc
     5281 ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca
     5341 cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt
     5401 gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct ccagcagccg
     5461 cacgcggcgc atctcgggca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct
     5521 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc
     5581 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac
     5641 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cccctacgtg
     5701 ctgctgaagt tgcccgcaac agagagtgga accaaccggt gataccacga tactatgact
     5761 gagagtcaac gccatgagcg gcctcatttc ttattctgag ttacaacagt ccgcaccgct
     5821 gccggtagct attgactatc cggctgcact agccctgcgt cagatggctc tgatccaagg
     5881 caaactgcca aaatatctgc tggcaccgga agtcagcgcc ctgcaccatt atgttccgga
     5941 tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc
     6001 gctggcattg accctgagtg atttttctct ggtgccgccc tatccctttg tgcagcttgc
     6061 cacgctcaaa ggggtttgag gtccaaccgt acgaaaacgt acggtaagag gaaaattatc
     6121 gtctgaaaaa tcgattagta gacaagaaag tccgttaagt gccaattttc gattaaaaag
     6181 acaccgtttt gatggcgttt tccaatgtac attatgtttc gatatatcag acagttactt
     6241 cactaacgta cgttttcgtt ctattggcct tcagacccca tatccttaat gtcctttatt
     6301 tgctggggtt atcagatccc cccgacacgt ttaattaatg ctttctccgc cggagatcga
     6361 cgcacagcgt tctgtgctct atgatgttat ttcttaataa tcatccaggt attctcttta
     6421 tcaccatacg tagtgcgagt gtccacctta acgcagggct ttccgtcaca gcgcgatatg
     6481 tcagccagcg gggctttctt ttgccagacc gcttccatcc tctgcatttc agcaatctgg
     6541 ctatacccgt cattcataaa ccacgtaaat gccgtcacgc aggaagccag gacgaagaat
     6601 atcgtcagta caagataaat cgcggatttc cacgtatagc gtgacatctc acgacgcatt
     6661 tcatggatca tcgctttcgc cgtatcggca gcctgattca gcgcttctgt cgccggtttc
     6721 tgctgtgcta atccggcttg tttcagttct ttctcaacct gagtgagcgc ggaactcacc
     6781 gatttcctga cggtgtcagt catattaccg gacgcgctgt ccagctcacg aatgaccctg
     6841 ctcagcgttt cactttgctg ctgtaattgt gatgaggcgg cctgaaactg ttctgtcaga
     6901 gaagtaacac gcttttccag cgcctgatga tgcccgataa gggcggcaat ttgtttaatt
     6961 tcgtcgctca tacaaaatcc tgtctatcgt gagaatgacc agcctttatc cggcttctgt
     7021 cgtatctgtt cggcgagtcg ctgtcgttct ttctcctgct gacgctgttt ttccgccaga
     7081 cgttcgcgct ctctctgcct ttccatctcc tgatgtatcc cctggaactc cgccatcgca
     7141 tcgttaacaa gggactgaag atcgatttct tcctgtatat ccttcatggc atcactgacc
     7201 agtgcgttca gcttgtcagg ctctttttca aaatcaaacg ttctgccgga atgggattcc
     7261 tgctcaggct ctgacttcag ctcctgtttt agcgtcagag tatccctctc gctgagggct
     7321 tcccgtaacg aggtagtcac gtcaattacg ctgtcacgtt catcacggga ctgctgcacc
     7381 tgcctttcag cctccctgcg ctcaagaatg gcctgtagtt gctcagtatc gaatcgctga
     7441 acctgacccg cgcccagatg ccgctcaggc tcacggtcaa tgccctgcgc cttcagggaa
     7501 cgggaatcaa cccggtcagc gtgctgatac cgttcaaggt gcttattctg gaggtcagcc
     7561 cagcgttccc tctgggcaac aaggtattct ttgcgttcgg tcggtgtttc cccgaaacgt
     7621 gccttttttg cgccaccgcg ctccggctct ttggtgttag cccgtttaaa atactgctca
     7681 gggtcacggt gaataccgtc attaatgcgt tcagagaaca tgatatgggc gtggggctgc
     7741 tcgccaccgg ctatcgctgc tttcggatta tggatagcga actgataggc atggcggtcg
     7801 ccaatttcct gttggacaaa atcgcggaca agctcaagac gttgttcggg ttttaactca
     7861 cgcggcaggg caatctcgat ttcacggtag gtacagccgt tggcacgttc agacgtgtca
     7921 gcggctttcc agaactcgga cggtttatgc gctgcccacg ccggcatatt gccggactcc
     7981 ttgtgctcaa ggtcggagtc tttttcacgg gcatactttc cctcacgcgc aatataatcg
     8041 gcatgaggag aggcactgcc ttttccgccg gtttttacgc tgagatgata ggaggccatc
     8101 gtgttttatc ccgctgaagg cgcgcaccgt ttctgaacga agtgaagaaa cgtctaagtg
     8161 cgccctgata aataaaagag ttatcaggga ttgtagtggg atttgacctc ctctgccatc
     8221 actgagcata atcattccgt tagcattcag gaggtaaaca gcatgaataa aagcgaaaaa
     8281 caggaacaat gggcagcaga aagagtgcag tatattcgcg gcttaaagtc gccgaatgag
     8341 caacagaaac ttatgctgat actgacggat aaagcagata aaacagcaca ggatatcaaa
     8401 acgctgtccc tgctgatgaa ggctgaacag gcagcagaga aagcgcagga agccagagcg
     8461 aaagtcatga acctgataca ggcagaaaag cgagccgaag ccagagccgc ccgtaaagcc
     8521 cgtgaccatg ctctgtacca gtctgccgga ttgcttatcc tggcgggtct ggttgacagt
     8581 aagacgggta agcctgttga tgataccgct gccttactgg gtgcattagc cagtctgaat
     8641 gacctgtcac gggataatcc gaagtggtca gactggaaaa tcagagggca ggaactgctg
     8701 aacagcaaaa agtcagatag caccacatag cagacccgcc ataaaacgcc ctgagaagcc
     8761 cgtgacgggc ttttcttgta ttatgggtag tttccttgca tgaatccata aaaggcgcct
     8821 gtagtgccat ttacccccat tcactgccag agccgtgagc gcagcgaact gaatgtcacg
     8881 aaaaagacag cgactcaggt gcctgatggt cggagacaaa aggaatattc agcgatttgc
     8941 ccgagcttgc gagggtgcta cttaagcctt tagggtttta aggtctgttt tgtagaggag
     9001 caaacagcgt ttgcgacatc cttttgtaat actgcggaac tgactaaagt agtgagttat
     9061 acacagggct gggatctatt ctttttatct ttttttattc tttctttatt ctataaatta
     9121 taaccacttg aatataaaca aaaaaaacac acaaaggtct agcggaattt acagagggtc
     9181 tagcagaatt tacaagtttt ccagcaaagg tctagcagaa tttacagata cccacaactc
     9241 aaaggaaaag gactagtaat tatcattgac tagcccatct caattggtat agtgattaaa
     9301 atcacctaga ccaattgaga tgt
//
