LOCUS       NZ_CP014115            29612 bp    DNA     circular CON 06-MAR-2024
DEFINITION  Staphylococcus saprophyticus strain FDAARGOS_168 plasmid unnamed2,
            complete sequence.
ACCESSION   NZ_CP014115
VERSION     NZ_CP014115.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03996313
            Assembly: GCF_001558275.2
KEYWORDS    RefSeq.
SOURCE      Staphylococcus saprophyticus
  ORGANISM  Staphylococcus saprophyticus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 29612)
  AUTHORS   Campos,J., Goldberg,B., Tallon,L., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J.,
            Nadendla,S., Nandy,P., Hobson,J. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 29612)
  AUTHORS   Campos,J., Goldberg,B., Tallon,L., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J.,
            Nadendla,S., Nandy,P., Hobson,J. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, US Food and Drug Administration,
            10903 New Hampshire Avenue, Silver Spring, MD 20993-0002, USA
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 29612)
  AUTHORS   Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Nadendla,S. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2015) Institute for Genome Sciences, University of
            Maryland, 801 W.Baltimore Street, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP3 v. Oct 2014
            Genome Coverage                   :: 674.083x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 03:49:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,646
            CDSs (total)                      :: 2,563
            Genes (coding)                    :: 2,494
            CDSs (with protein)               :: 2,494
            Genes (RNA)                       :: 83
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 28 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 10 of 69
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP014115.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..29612
                     /organism="Staphylococcus saprophyticus"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_168"
                     /isolation_source="Urine"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_168"
                     /db_xref="taxon:29385"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: DC"
                     /collection_date="2014-06-19"
                     /collected_by="Children's National Hospital"
     gene            join(29292..29612,1..483)
                     /locus_tag="AL528_RS13000"
                     /old_locus_tag="AL528_12850"
     CDS             join(29292..29612,1..483)
                     /locus_tag="AL528_RS13000"
                     /old_locus_tag="AL528_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002452565.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_215051629.1"
                     /translation="MLVISYIFYFATEPFFTLCNKLKEQDTRKLEIPFDELKHLSNYYS
                     RSQARFIKDLEHVYDKMLHLTYTERNEQSFEKFVLFTSYKVDIKKQYLSISVNNDLKHI
                     LNSITADFTKFELQEMTQLKSTYAKNMFRLLKQYKHTGYFKIQINDFRERLDIPKSYRM
                     SEIDKYVFKPIIKELGFLFKNLNINKIKAKKGRKIEWLEFSFEPEKRIHSKRQSNMIST
                     GKPKQYISREMTPQWLKNNTYQPTTSKTSAYTEEERQAFLQKMKK"
                     /name="RepB family plasmid replication initiator protein"
     gene            502..987
                     /locus_tag="AL528_RS13005"
                     /old_locus_tag="AL528_12855"
     CDS             502..987
                     /locus_tag="AL528_RS13005"
                     /old_locus_tag="AL528_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_002440848.1"
                     /translation="MKTVKEVSELLDVSKQTIHYHLKSLPSNLKVNKVGNKSLIDDNTI
                     DYLKTILNKKTTKESTKFDEKSTNKTESLNNQYSSYIDKYIEHLESEIRQKNRQIDDLT
                     IALKQEQSLNLNSQNILSNTKKIETDNIDSKEDIITTKASKKNEQKQSIFSKLFKKK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            1350..2291
                     /locus_tag="AL528_RS13010"
                     /old_locus_tag="AL528_12860"
     CDS             1350..2291
                     /locus_tag="AL528_RS13010"
                     /old_locus_tag="AL528_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449936.1"
                     /GO_function="GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="WP_001831389.1"
                     /translation="MENDHNHNHTHGANKKTLLISFIIITSYMIVEGLGGFFTNSLALI
                     SDAGHMLSDSISLGIALIAFTLGAKQANTNKTFGYKRFEILAAVLNGITLMLIAIYIFY
                     EAIERFKNPPEVASTGMLIIALVGLFINIIVAWIMLRGSDVEENLNMRGAYLHVISDML
                     GSIGAVIAALLIIFFRWGWADPLASVIVAILVLRSGFYVTKSSLHVLMEGAPSNINTKD
                     IIKTIKKFKEVKNIHDFHVWSVTSGLNALSCHIVVEDTMTITENEFLLKRIEHELNHQN
                     IQHVTIQTETSNNNHSEKLFCIVKEEDSQHHH"
                     /name="cation diffusion facilitator family transporter"
     gene            complement(2332..2607)
                     /locus_tag="AL528_RS13015"
                     /old_locus_tag="AL528_12865"
     CDS             complement(2332..2607)
                     /locus_tag="AL528_RS13015"
                     /old_locus_tag="AL528_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489264.1"
                     /GO_function="GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="WP_002489264.1"
                     /translation="MDKNIQLELYTRPTCSDCQESKRYLNSQEISYSHKDVSQNLDLEE
                     EMKKISGNRIVPLFVFYKKNFWGKKKLFKYYVGFENNKKDILNILE"
                     /name="glutaredoxin family protein"
     gene            2782..3057
                     /locus_tag="AL528_RS13020"
                     /old_locus_tag="AL528_12870"
     CDS             2782..3057
                     /locus_tag="AL528_RS13020"
                     /old_locus_tag="AL528_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831396.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0046872 - metal ion binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="WP_001831396.1"
                     /translation="MNTTTVKNNDALLNRLKRLEGQMRGLQSMIAEDRYCIDVLVQITA
                     IQSALKQVGFIVTEDHISNCVSEGIKNGNGEESIKELMVVLKQFSK"
                     /name="metal-sensing transcriptional repressor"
     gene            3072..5459
                     /locus_tag="AL528_RS13025"
                     /old_locus_tag="AL528_12875"
     CDS             3072..5459
                     /locus_tag="AL528_RS13025"
                     /old_locus_tag="AL528_12875"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485948.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0019829 - ATPase-coupled monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="WP_001832797.1"
                     /translation="MSENIKKTSLGITGMTCAACSNKVEKNLNKLDEVNANVNPSTEKA
                     TIEYNPNVTSLEDIANTIQKTGYGVLTEKVDLDVMGMTCAACSNKIEKVLNRISGVNKA
                     TVNLTTESATVEYNPDMTSVDEFQQRIKNLGYEAQPKKEASEKSSQKEKQLKRQLIKLV
                     VSAVLAAPLLMTMFVHLFGIQIPHIFMNPWFQFVLATPVQFVIGWQFYVGAYKNLRNGS
                     ANMDVLVALGTSAAFFYSIYESIKWLINTNYEPHLYFETSAVLITLILFGKYLEARAKT
                     QTTNALSKLLNLQAKEARILRNGEETMVPLSEVKEGDYLVIKPGEKIPVDGKIIKGMTS
                     IDESMLTGESIPVEKMQNDNVIGSTMNKNGAITVEATKVGKDTALASIVKVVEEAQGSK
                     APIQRLADIISGYFVPIVVGIAIFTFIIWISLVQPGQFEPALVAAIAVLVIACPCALGL
                     ATPTSIMVGTGKAAENGILFKGGEHIEGTHAINTVVLDKTGTITNGTPEVTDFSGDDQT
                     LQLLASAEKGSEHPLAEAIVSYAKEKSLEFLEVDHFEAIPGRGINATIDGKELFVGNRK
                     LMSEKGIQTNEAETNLAQFEKEGKTAMLISVDNELRGVVAVADTVKDTAQQAIQKLHEL
                     GIEVAMLTGDNKRTAQAIAKQVGIDTIIAEVLPEEKASKVAEIQSEGKKVAMVGDGVND
                     APALVKADIGIAIGTGTEVAIEAADITILGGDLLLIPKAIKASKSTIRNIRQNLFWAFG
                     YNVAGIPIAAIGLLAPWVAGAAMALSSVSVVTNALRLKRMKL"
                     /name="heavy metal translocating P-type ATPase"
     gene            5493..5702
                     /gene="copZ"
                     /locus_tag="AL528_RS13030"
                     /old_locus_tag="AL528_12880"
     CDS             5493..5702
                     /gene="copZ"
                     /locus_tag="AL528_RS13030"
                     /old_locus_tag="AL528_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831373.1"
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="WP_001831373.1"
                     /translation="MTNEVINVEGMSCDHCKHSIEKALNGLDGVTSSEVSLANGNVEVE
                     FDENQVAFNDFKEAIEDQGYDVIK"
                     /name="copZ"
     gene            5766..6422
                     /locus_tag="AL528_RS13035"
                     /old_locus_tag="AL528_12885"
     CDS             5766..6422
                     /locus_tag="AL528_RS13035"
                     /old_locus_tag="AL528_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001831384.1"
                     /translation="MNHNHEYHSNKPEIKANVVYRDGNIEITLEDEFNNAPLLDTMHEK
                     EMHFVLVSNDMEKYYHLHPQKKHEGLFIINQQLEPGTYQAFVDVTPKNHVYSVHPIELQ
                     IGDKITSTTSLNSDKNWVKVNKGVHVTLNSISAKEDEHVPLTFNTELTPQPYLGALGHV
                     IIFDEQLTDYIHVHPESPDSTTFYAHFPKKGMYKIWAEFKFNDEVHRFTYNIKVA"
                     /name="hypothetical protein"
     gene            6615..7142
                     /locus_tag="AL528_RS13040"
                     /old_locus_tag="AL528_12890"
     CDS             6615..7142
                     /locus_tag="AL528_RS13040"
                     /old_locus_tag="AL528_12890"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="WP_002440835.1"
                     /translation="MIKFEYFEEKDFDLLINWINSPELLVKWAGTDFNYPLDEEQLKEY
                     IKDTNKENSSKIIYKVIIEENNSKKIIGHIALNNIDFKNCSATLGKVLVGDPQMRGQGL
                     GPKIIKKLLKKAFEDLGLHRIELGVFAFNKSAIKSYKKIGFIKEGEKRDFRKLNGEYYN
                     LWQMSMLESEWS"
                     /name="GNAT family protein"
     gene            complement(7240..9012)
                     /locus_tag="AL528_RS13045"
                     /old_locus_tag="AL528_13255"
     CDS             complement(7240..9012)
                     /locus_tag="AL528_RS13045"
                     /old_locus_tag="AL528_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="WP_002440832.1"
                     /translation="MKSKKSLEKLYDDLFSTNFLINYGYFDITPKNNQDITDLNSYGKS
                     DAFYLKIFNMSKLAEYISDNPFYTMKMFNDKKVYSTEPITFTIPKSNISRREYKIPNIY
                     SYLNLMFFIQKNKEEFKRIFLANKFSTSKFFSLADFDFKFTDNLKKTLLFGGNHILNVD
                     LSNFYHSLYTHSIPWVIMGKKNAKKERDKGFSNKLDKLIASCQYNQTHGIPTGNILSRI
                     ISELYMCYIDSEMENKGYRYARYVDDISFPFNFEEEKDKFYRDFNKLCMKYELKINDKK
                     TEINDFPYIHTQNKDFIFNYFKNYSSDSKEETWIIGIKNFIDLCIDEERKGNKGAIKCI
                     FPVIENTFKYKKINKNKISKIFGYTNNITKFNILQFILDLSLKDSKLTNRCLSLLNYLA
                     VKMDDKKVVSKQVKQYFRNRNEEIKNLLVFYNKNDYHQETYQILVYIVEYDVDILLKKN
                     VLNLLNENTDNLSLSLLTIIYLRKPWRIESLLKKIDDLFKNSKDSYPEKIGVMSQNLWY
                     FRYFIYYLIKENVIPKDKINSYCNSQSYRSNQKGYKSDLNWRYINSNDAVDEFFNELLE
                     EKVPLIDLEYVNLI"
                     /name="RNA-directed DNA polymerase"
     gene            complement(9164..9259)
                     /locus_tag="AL528_RS13165"
     CDS             complement(9164..9259)
                     /locus_tag="AL528_RS13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_002441941.1"
                     /translation="MLMIFVHIIAPVISGCAVAYFTYWLSSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            9586..9807
                     /locus_tag="AL528_RS13050"
                     /old_locus_tag="AL528_12905"
     CDS             9586..9807
                     /locus_tag="AL528_RS13050"
                     /old_locus_tag="AL528_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002440830.1"
                     /translation="MSKEKITREKTSIKIHRTFKPLFDELVAEERDSKIDFMDALLVFY
                     AKENHPEILEAFKNQELKTQKNEKAKDL"
                     /name="hypothetical protein"
     gene            complement(11030..11305)
                     /locus_tag="AL528_RS13055"
                     /old_locus_tag="AL528_12910"
     CDS             complement(11030..11305)
                     /locus_tag="AL528_RS13055"
                     /old_locus_tag="AL528_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440828.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_002440828.1"
                     /translation="MELISKLIQEDINNDSELETLYKEQGEKLEFSIAVVSLRKDIGWS
                     QMQFAQKLKVPQTTIEIIENGDVLPTMSLLRSVADVTEKKLCISFV"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(11394..11741)
                     /locus_tag="AL528_RS13060"
                     /old_locus_tag="AL528_12915"
     CDS             complement(11394..11741)
                     /locus_tag="AL528_RS13060"
                     /old_locus_tag="AL528_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119677.1"
                     /translation="MSYENACDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNDVLDFYKEGKMAYYFIKDDEI
                     REFFSKNQEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(11760..12377)
                     /locus_tag="AL528_RS13065"
                     /old_locus_tag="AL528_12920"
     CDS             complement(11760..12377)
                     /locus_tag="AL528_RS13065"
                     /old_locus_tag="AL528_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="WP_002440820.1"
                     /translation="MIQTIVTAAILYIATAVDLLVILLIFFARAKTRKEYRDIYIGQYV
                     GSVALIVVSLFFAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNEKG
                     LSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLITLFVFLILIFFLVFTAQKLAN
                     IPGVGEIVEKFSRWIMAVIYIALGLFIIIENDTIQTILGFIF"
                     /name="CadD family cadmium resistance transporter"
     gene            complement(12614..12757)
                     /locus_tag="AL528_RS13345"
     CDS             complement(12614..12757)
                     /locus_tag="AL528_RS13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002324169.1"
                     /translation="MKKTERNLFSIFLSLGSLFAIFSGFFILGIGMLIGGATLLTKSIS
                     EE"
                     /name="hypothetical protein"
     gene            complement(13413..>13715)
                     /locus_tag="AL528_RS13070"
                     /old_locus_tag="AL528_13260"
                     /pseudo=""
     CDS             complement(13413..>13715)
                     /locus_tag="AL528_RS13070"
                     /old_locus_tag="AL528_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001568503.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            complement(13732..14703)
                     /locus_tag="AL528_RS13075"
                     /old_locus_tag="AL528_12930"
     CDS             complement(13732..14703)
                     /locus_tag="AL528_RS13075"
                     /old_locus_tag="AL528_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_002440813.1"
                     /translation="MSNFNIKEIQKEKFYQLPKVFFTNPKYVNLSNDAKITWSILRDRL
                     DLSIRNNWVDKNGDIFFIYTNEKLKSILNISSPNKLSKIKKELTQADLFNQIRVGLNKP
                     NKLYIKKPEVTEADVYYISQQENDIEQRNNTDVSNSYVQKYDNDTSRNINLIHHDISKE
                     YTNDTDLSNTDYIETKNNDTHDMNDTYNNSTNHNHSNHTNHQQNEFNNDALKFQVLEEL
                     PQQIKDYVSNFEIREIRIIKSVLLKGKKSFNNAHDTYYRLEDVEFEIVSVLKRFKAMLL
                     QKNETVEAMQGYLMQSIKAELEEIHALNMRRQNMKQHNIFSQ"
                     /name="replication initiator protein A"
     gene            15218..15904
                     /locus_tag="AL528_RS13080"
                     /old_locus_tag="AL528_12935"
     CDS             15218..15904
                     /locus_tag="AL528_RS13080"
                     /old_locus_tag="AL528_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002440810.1"
                     /translation="MKSMKEIADELNVTKMTVYNNAKKANVKFQKIDNVNYLSGEDETI
                     VVNRIRKNQNKNDDFEGEKKEEAEPNNDNLVKDETIKQLYNQVAIYKEQSNRDKEQINT
                     LNRLLENQQILALESNKKIQKLENQLEEERQLNYSFDTSSNDRQNVKAQEAKFTEETKD
                     FNQQPEKEQPIEVQHKDIPEEKENENLNEEPSTIDDKQFEEIDNEYEDKEEKSPKKGFW
                     SRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            complement(16082..17215)
                     /locus_tag="AL528_RS13085"
                     /old_locus_tag="AL528_12940"
     CDS             complement(16082..17215)
                     /locus_tag="AL528_RS13085"
                     /old_locus_tag="AL528_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505014.1"
                     /GO_function="GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="WP_047511863.1"
                     /translation="MTNNLDDIFSDDLFDDLTKQVKKKKAEKLDPEIIKFQEILNFVKE
                     NDREPNKTDEWSEERALWARLQGFREKKERLDKVKHLDELNLLDKKGNYSDRMNLDNNA
                     NQVNNLNEVLEDENLFDDFSDLLDVSRYKRTVNAASKKSSRKHATNFDKYQKLFEKVHE
                     DIANGKRKIIPFEKYDIEAGRFYIQNGVMLYIVSISSEKFTGDNGKENALMHVVYENGT
                     ENKQLLLQSLASSLYSKERHGRMVTNIVDDESLSERFGAEFTTGYIYVLKSLSENPEIR
                     KINNLYKIGFTRNRIESRIVNAENEVTYLNAPVRLVLSAEVKNLNAQLLERTLHHAFQD
                     KQVIFQDSAYKKATEWYIVSIDEIKEKINKILYDIQM"
                     /name="GIY-YIG nuclease family protein"
     gene            complement(17205..19163)
                     /locus_tag="AL528_RS13090"
                     /old_locus_tag="AL528_12945"
     CDS             complement(17205..19163)
                     /locus_tag="AL528_RS13090"
                     /old_locus_tag="AL528_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0003676 - nucleic acid binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="WP_047511860.1"
                     /translation="MTDNLFEINFDETQSKSTNSLGMRDMQARVYEKRNSNYLLVKAPP
                     ASGKSRALMFVGLDKLKNQDIKKVIIAVPERSIGKSFRNTDLQKYGYYWDWHVNPNNNL
                     TTTVGRKSKVKHFVNFMNSNNPNDDIIILTHATLRFAFEELDDSYFDNSLLAIDEFHHV
                     SREDSSVLGNALRSIMNNSTAHILAMTGSYFRGDSTQILEPSDEDKFDKVTYTYYEQLE
                     GYQYLKSFAMGYSFYRGRYTEALDEVIDVTKKSIIHIPNVNSGESTKDKYNEVDQIMDI
                     ISDGGEVLQNKDGIYEISRPDGKHLLVADLVNEESREKVTSYLSNISDDIEDLDKLDII
                     IALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKNHAQFTNLIAQPDAQ
                     DDEVLYAVNSIMKAISASLLMEQVLAPVYKFKPKDEKKYSSDNELSIQGLNPSNTTERT
                     KKIIEDDMSDLKSSILQSQDIQNAIVSGSDAKTINKTLIPRVIIERYPELTTEELETVR
                     QHAVADINVSAGSKTSSEDGNREIIKMADRFINIDELNIDLIDSVNPFQRAYEMISRDI
                     NASTLQFVQDYISSKKYEFTQEQLIDAYQRAKQFRSENGHNPDRNSKDEGERYLAFALL
                     RLAEIKHEREVEQENDE"
                     /name="DEAD/DEAH box helicase"
     gene            complement(19168..21939)
                     /locus_tag="AL528_RS13095"
                     /old_locus_tag="AL528_12950"
     CDS             complement(19168..21939)
                     /locus_tag="AL528_RS13095"
                     /old_locus_tag="AL528_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="WP_047511857.1"
                     /translation="MVKKKTLSITEIENGIKDLINNLTEKEFITNFLMLYDIPKTSITR
                     AKAKFDKGEPFVIKNKLHYTETTGEVVSAIDAIEHKIQDQKSKPRYIIANDYINIAALD
                     IQTRDTLNIPFEELPSKADFFLAWNGIEKADYQSEHPADRKAAERFAKIYDVLAKDNPN
                     SDEHSFNLFLIRVLFLLFAEDTGIMEKGVFTNTLKTRTNEDGSNFNDVIKDLFEILNIN
                     ESNRFGKFNWLKVFPYVNGKLFDEGHIPLTFTNVSRKLLIEAGELLNWNEINPDILGSM
                     IQSVASPENRHSSGMHYTSVSNIMKVIKPLFLDELKNEFEKLYMSYKKITNKNITNKTR
                     QENKRSILKSLGKLHTRISNIKFLDPASGSGNFLIITYKEIRRLEIKIILLEQEIEQSG
                     QIPMSSIRLSQFNGIEIDDFAHEVAKISLWIAEHQMNEEMVKVIPSANPALLPLKDAGN
                     IIRGNSLRIDWNEVVSHKPNDEVYIMGNPPYLGAKLQNKQQKSDLSFVLGDEINSKKVD
                     FITGWFYKGAQFIRNSNNRVAFVSTNSINQGEQVSLIWPKLFEYSKLSFAYTSFKWKNN
                     AKSNAGVTVSIIALESKDRETISYLYSEHGKQMATVINPYLTIAPNIIVTSQNESISGF
                     PKAVFGSMPRDGGNLIFSEEEKNELFDKYTNNIVLRKYIKYYIGAHEFINDIFRYTLWF
                     ETHSQYNELSDIPEIEKRINKVRKMRLESKATSTREAGKVPYRFVQNGERVKYFEVYRK
                     KHNGSDTGIKQIIIPRVSSESRDYVPMGYVNNNTVISDSAIVIYNAPIWLLGLLQSRMH
                     MVWLRSIGGKLETRYRYSSGLVYNTFPVQNLSTQRKNEIARVMLDILDIREYEGGSLAE
                     LYNTKTMPKSLRQKHEELDGIIDRAYRQKPFNSDEERLSILLELYQNMTKGD"
                     /name="DNA methyltransferase"
     gene            22497..23600
                     /locus_tag="AL528_RS13100"
                     /old_locus_tag="AL528_12955"
     CDS             22497..23600
                     /locus_tag="AL528_RS13100"
                     /old_locus_tag="AL528_12955"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_049382801.1"
                     /translation="MIKVSKIIKQERLRRGMTQEALAEYLNTTKTTISKWENATLYPDI
                     TMLPKLAKVFNISVDDLLNYSITMTDEEIKEVSISLSKMINKTSYEEYVSTVRDYYISH
                     CNEFKFLNSLLGILANNILYCENETQVKETTKLAYEIIKTTEDNSESIYLKRHAQGYQT
                     LMYMFEGDYSSVIDNTPDFDLKIGENAILTLAYLQKGNISKAKNVIQTDMYQSLMIYMH
                     DFALMLTYDLYSIPIEYLESKLHYINKAFNVDYLYPHLSMNCYYKFALYYVKNDDNKAI
                     QYLKQYCQCFDYLTQDFVYQKDEFFNEIDEWLNTLPLENRLPANYKNTLNTAISRILEN
                     EDFMTLDNFTSIEEDVYRIYNEGMKNN"
                     /name="helix-turn-helix transcriptional regulator"
     gene            23600..24427
                     /gene="yidC"
                     /locus_tag="AL528_RS13105"
                     /old_locus_tag="AL528_12960"
     CDS             23600..24427
                     /gene="yidC"
                     /locus_tag="AL528_RS13105"
                     /old_locus_tag="AL528_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002501677.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /GO_process="GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="WP_047511866.1"
                     /translation="MHRKKLIVLLLLSFTLLSACDYSKSENRNGFFYHTFAVPMDHLLH
                     WLGSIFDNNYGLAIITIVLIVRIIMLPFMLAQSKNGHLMRKKMEIVKPELTKIQEKIKK
                     ANTQEEKMTANQEMMDKYKEYNMNPMKTMLGCLPLLIQMPVLFGLYVSLKWPSSGGLTE
                     HTDFLWFNLTHPDIWITIIAGILYIIQPLINMENMPKEQRSMGYIMAIISPIFIIYISI
                     TSASALGLYWTINAAFLIIQMFFSNRYYSRLATKEANQLKRKVENNKLATKYT"
                     /name="yidC"
     gene            complement(24568..25176)
                     /locus_tag="AL528_RS13110"
                     /old_locus_tag="AL528_12965"
     CDS             complement(24568..25176)
                     /locus_tag="AL528_RS13110"
                     /old_locus_tag="AL528_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_047511854.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRPV
                     FQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGNPL
                     LDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKDKGIYKGRPLLYSPNAKDPQKRI
                     IYYRVVEMLEEGQAISKIAKEVNITRQTVYRIKHDKGLS"
                     /name="recombinase family protein"
     gene            complement(25662..26561)
                     /locus_tag="AL528_RS13115"
                     /old_locus_tag="AL528_12970"
     CDS             complement(25662..26561)
                     /locus_tag="AL528_RS13115"
                     /old_locus_tag="AL528_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="WP_000196697.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVEW
                     VLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATIDPE
                     IQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRRMNK
                     VKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDWTEAT
                     HSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIAEFAME
                     SGESAYETMALKELGMKE"
                     /name="Mph(C) family macrolide 2'-phosphotransferase"
     gene            complement(26660..28126)
                     /gene="msr(A)"
                     /locus_tag="AL528_RS13120"
                     /old_locus_tag="AL528_12975"
     ARG             complement(26664..28126)
                     /name="msr(A)"
                     /gene="msr(A)"
                     /gene_name="ABC-F type ribosomal protection protein Msr(A)"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE/STREPTOGRAMIN"
                     /identity="99.8"
                     /coverage="100.0"
     gene            28600..29274
                     /locus_tag="AL528_RS13125"
                     /old_locus_tag="AL528_12980"
     CDS             28600..29274
                     /locus_tag="AL528_RS13125"
                     /old_locus_tag="AL528_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_001105960.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TIYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDTEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVVTDQAPSTKAAMAKIIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6 family transposase"
     ARG             complement(25666..26561)
                     /name="mph(C)"
                     /gene="mph(C)"
                     /gene_name="Mph(C) family macrolide 2'-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(26661..28126)
                     /name="msrA"
                     /gene="msrA"
                     /gene_name="msr-type ABC-F protein"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; streptogramin
                     antibiotic; streptogramin B antibiotic"
                     /identity="98.57"
                     /coverage="100.0"
     ARG             complement(25663..26561)
                     /name="mphC"
                     /gene="mphC"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="98.66"
                     /coverage="100.0"
     Typing          complement(13414..13601)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          complement(13733..14703)
                     /name="rep_cluster_1110"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attactgctg atttcactaa atttgaacta caggaaatga cacaactaaa atcaacttat
       61 gcaaaaaata tgtttagatt actaaagcaa tataaacata caggatattt caaaattcaa
      121 attaatgatt tcagagaacg tttagatatt ccgaaaagtt atcgaatgag tgaaattgat
      181 aagtatgtat ttaaaccaat aattaaagaa ttaggctttt tatttaaaaa tctcaatatt
      241 aataaaatta aagctaaaaa aggacgaaag attgaatggt tagaattttc ttttgaacct
      301 gagaaacgta ttcattcgaa acgacaatct aatatgataa gtacaggcaa accaaaacaa
      361 tatataagcc gtgaaatgac gccacaatgg ttaaaaaata acacttatca accaaccaca
      421 tctaagacat ctgcgtatac agaagaagaa cgtcaagcgt ttttacaaaa aatgaaaaag
      481 taaaggagac ccagttattt gatgaaaact gtaaaggaag tttctgaact tttagatgtc
      541 tctaaacaaa caattcatta tcatttaaaa agtttacctt caaatttgaa ggtaaacaaa
      601 gtaggtaaca aatcattaat agatgataat acaatagatt atctaaaaac aattttaaat
      661 aaaaaaacga caaaagaatc gacaaaattt gatgaaaaat cgacaaacaa aacagaatca
      721 ttaaataacc aatatagcag ttatatcgat aaatacatag agcatttaga atcagaaatt
      781 cgacaaaaga atcgacaaat tgatgattta acaattgcat taaaacaaga acaatctttg
      841 aatttaaata gccaaaatat attaagtaat accaaaaaaa tagaaactga taatatagat
      901 tcaaaagaag atatcattac tacaaaagca tcaaaaaaaa atgaacaaaa acaatctatc
      961 ttttctaaac tattcaaaaa aaaataatgt attcacgcct ttaggcgtga atacattatt
     1021 ttctctaaaa agaaattatc acttgttaat tataatatag ttaatattta gcaagtgaag
     1081 tgtggtttat catatgcaaa aatcccacta ctgtccaaag caaagtaggg tttttattta
     1141 atatacaaaa tacaaagcat ttctatattg acaaaggata aaaatcatac taacttatga
     1201 atatatgagc atatattcat aagttagtat gattttcttc gagtaacaaa acattaattg
     1261 aaattttata ttttttaact ctatagttat aaaaacaaaa attcaaatta tagaaaatat
     1321 ttttcatcat taaaaagagg agggattaaa tggaaaacga tcacaatcat aatcatacac
     1381 atggcgcgaa caaaaagact ttattaatta gttttattat tattacaagt tacatgattg
     1441 tagaagggtt aggtggtttc tttactaaca gtcttgcgtt aatttcagat gctggtcata
     1501 tgttaagtga ttctatttct ttaggtattg ctttaattgc atttacttta ggagcgaagc
     1561 aagctaatac aaataaaact tttggctaca aaaggtttga aatactagca gctgtactta
     1621 atggtattac tttgatgtta atagctatct atatttttta tgaagctatt gagagattta
     1681 aaaacccccc tgaggtagct tctacaggca tgttaattat cgccttggta ggcttgttta
     1741 ttaatattat tgtggcttgg ataatgctgc gtgggagcga tgtagaagaa aacttaaata
     1801 tgcgtggagc atatttgcat gtaataagcg acatgcttgg atctataggt gcagttatag
     1861 cagcccttct cattatattt tttagatggg ggtgggcgga tcctttagca agtgtgattg
     1921 tagcaatttt agtactacgt agcggctttt atgtaacaaa atcaagtctt catgtattaa
     1981 tggagggagc accaagcaat ataaatacaa aagacattat taaaactatt aaaaaattca
     2041 aagaagttaa aaatattcat gactttcatg tttggtcagt aactagcgga ttaaacgcgt
     2101 tatcttgtca tattgttgta gaagatacaa tgaccattac tgaaaatgag tttttactta
     2161 aacgtataga acatgaactc aatcatcaaa atattcaaca tgtcacaata cagactgaaa
     2221 cttctaacaa taatcatagt gaaaaattgt tttgtatcgt gaaagaagaa gacagtcaac
     2281 atcatcatta aataaacagc cattattttt taataatggc tgtttattta attattccaa
     2341 tatatttaat atatcttttt tgttattttc aaatcccacg taatatttaa acaacttttt
     2401 tttaccccaa aaattttttt tataaaaaac aaataaggga acaattcggt tgcctgatat
     2461 ttttttcatt tcttcttcta agtccaaatt ttgacttaca tctttatgac tataactaat
     2521 ctcttgagag tttaaatacc tttttgattc ttggcaatca gagcatgtag gtcttgtgta
     2581 aagctcaagt tgtatatttt tatccaaata tatctctcct aatataagtt ttagcataca
     2641 ttttatacaa gtgtacctct taaaacaaac tataaatatt aattttttat atgaaacttt
     2701 tgtattgact tatacctgat gggggtataa tatagattcc aacaagagat accccgttag
     2761 ggtatatgag gaggggtgaa attgaacact actactgtta aaaacaatga cgcattgctc
     2821 aatagactca aaagattaga aggacaaatg cgcggtttac aatcaatgat agcagaagat
     2881 agatattgta tagatgtact agtacaaata acagcaatac aatcagccct aaaacaagtg
     2941 ggttttatag taactgaaga ccatatatct aattgtgtaa gtgaaggtat taaaaatggc
     3001 aacggagaag aaagtatcaa agaattgatg gttgtattaa aacaattttc aaaataggag
     3061 gaaagaaata attgagtgaa aatattaaaa aaacctcatt aggtatcaca ggcatgacat
     3121 gtgcagcttg ctcgaataaa gtagaaaaaa atctaaataa attagatgaa gttaacgcta
     3181 atgtaaatcc ttctacagag aaagctacaa ttgaatataa ccccaatgtt acatcattag
     3241 aagatattgc aaatacaatt caaaaaacag gttatggagt tttaaccgaa aaagtggatc
     3301 tcgatgtaat gggaatgacg tgtgcagctt gttcgaataa aatcgaaaaa gttttaaatc
     3361 gaatctcagg tgtaaataag gctacagtta atttaaccac agaaagtgct actgtagaat
     3421 acaaccctga tatgacgtct gtagatgaat ttcaacaacg tattaaaaac ctagggtatg
     3481 aggcacaacc taaaaaagaa gcatcagaaa aaagttccca aaaagaaaaa caattaaaac
     3541 gacagttaat taaattagtt gtttcagctg ttttagcagc tccattgtta atgacaatgt
     3601 tcgttcattt atttggtatt caaataccac atatatttat gaacccgtgg ttccaatttg
     3661 tattagcaac gcctgttcaa tttgttattg gttggcaatt ttatgtaggc gcttataaaa
     3721 accttcgtaa tgggtcagca aatatggatg ttcttgtcgc tttaggtaca agcgcagcct
     3781 tcttttatag tatttatgaa tcgattaagt ggttaattaa tacaaattat gagccacacc
     3841 tatattttga aacaagtgct gtattaatta ctttaattct tttcggaaag tatttagaag
     3901 cacgtgctaa aacacaaaca acaaatgcgt taagtaaatt gttgaattta caagctaaag
     3961 aagcacgtat attacgcaac ggtgaagaga ctatggttcc tttaagtgag gtaaaagaag
     4021 gagactattt agttatcaaa ccaggagaaa aaataccagt agatggcaaa ataattaaag
     4081 gtatgacttc aattgacgag tcaatgttga caggtgaatc tattcctgtt gaaaaaatgc
     4141 aaaatgataa tgttatagga tcaacaatga ataaaaatgg agcaattact gttgaagcaa
     4201 caaaagttgg taaagataca gctcttgcct ccattgttaa agttgtcgaa gaagcacaag
     4261 gttctaaagc gccaatacaa agactagcag atattatttc tggatatttt gtacctatcg
     4321 ttgtaggtat cgcgattttc acatttatta tatggatttc attagttcag ccgggacaat
     4381 ttgaaccagc cttagttgct gcaatagcag tattagttat cgcttgtcct tgtgcgttag
     4441 gcttagctac gcctacttct ataatggtgg gtaccggaaa agctgctgaa aatggcattc
     4501 tatttaaagg tggagaacac attgaaggta cacacgctat taatacagtt gttttagata
     4561 aaactggcac tataacaaat ggcacacctg aagtcactga ttttagtgga gatgatcaaa
     4621 cattacaatt gttagctagt gcagaaaaag gttctgaaca cccactagct gaagctatcg
     4681 taagttatgc taaagaaaaa tcattagaat ttttagaagt agaccatttt gaagctatac
     4741 cagggcgcgg tataaatgca acaattgatg gtaaagaact ttttgtcgga aatcgaaaac
     4801 taatgagtga aaaaggaatc caaactaatg aagctgaaac aaatctcgct caatttgaaa
     4861 aagaaggaaa aactgcaatg cttataagcg tagataacga attgagaggg gttgtcgctg
     4921 tagctgatac agttaaagat actgcccaac aagctattca aaaacttcat gaattaggta
     4981 ttgaagttgc tatgttaact ggtgataaca aacgtaccgc acaagcaata gcaaaacaag
     5041 ttggtataga cacgattatt gcagaggttt taccagaaga aaaagcttct aaagtagcag
     5101 aaattcaatc tgaaggtaaa aaagtagcta tggttggaga tggtgtcaat gatgctccag
     5161 cactagttaa agcagatatt ggtattgcca ttggtacagg tacagaagtt gcaatcgaag
     5221 ctgctgatat aactatttta ggaggagacc ttttattaat tccaaaagca ataaaagcaa
     5281 gtaaatctac aattcgtaat attcgccaaa atctattttg ggcgtttggt tacaatgttg
     5341 caggtatccc tatagcagca attggtctgc tagcaccttg ggttgcggga gcagcaatgg
     5401 ctttaagttc tgtaagtgtt gtcacaaacg ctttacgtct aaaacgtatg aaattataag
     5461 aatcttatat ttcttgaaag gaggtgacaa cattgactaa cgaagttatt aatgtagaag
     5521 gtatgagctg tgaccattgc aagcattcaa ttgaaaaagc attaaatgga ttagatggtg
     5581 taacatcttc agaagtaagc ttggcgaatg ggaatgtgga agttgaattt gatgaaaatc
     5641 aagtagcttt caacgacttt aaagaagcta ttgaagatca aggttacgat gtaattaaat
     5701 aatttaaata gttgggcata tcgtttaatg atatgccctt attaaaaatt aaggaggata
     5761 aaaatatgaa tcataatcat gaatatcatt caaacaaacc cgaaattaaa gcaaatgttg
     5821 tatatagaga tggtaatata gaaattacgt tagaagatga atttaataat gccccattat
     5881 tagatactat gcatgaaaaa gaaatgcatt tcgttttagt atctaatgac atggagaaat
     5941 attaccacct acaccctcaa aaaaagcatg aaggtttatt tataattaac caacaattag
     6001 agccaggaac ttaccaggct tttgttgatg ttacaccaaa aaaccatgtg tattccgttc
     6061 atcctattga actgcaaatt ggagataaaa taacctctac aacatcttta aattctgata
     6121 aaaattgggt aaaagttaac aaaggagttc atgtaacttt aaattctata agtgctaaag
     6181 aagatgaaca tgtcccttta actttcaata cagaattaac tccccaacct tatttaggag
     6241 ctttaggaca tgtgattata tttgatgagc aattaactga ttatattcat gttcacccag
     6301 aatcacctga ttctacaacc ttttatgctc attttcctaa aaaagggatg tataaaattt
     6361 gggcagaatt taaatttaat gatgaagtgc atagatttac ttataatatt aaagttgcat
     6421 aaatctatta gcaaaagctt aaaaatcagt aaattgtaaa gagttattta gataatacaa
     6481 aacgctttat aaaaaatcga aaatttaaga tagaaagttg atgaagaaag tatcaaaaaa
     6541 ttgtagtggt tgtttatatt gcaacattca ctgaattttt atagttaata acctattatt
     6601 taaggagtaa tttattgatt aagtttgaat attttgagga gaaagatttt gatttattaa
     6661 taaactggat aaactctcct gaacttttag tcaaatgggc aggtacagat tttaattatc
     6721 ccctagatga agaacaatta aaggaatata ttaaagacac aaataaagaa aattctagta
     6781 aaataattta taaagttatc attgaagaaa ataattcaaa aaaaattata ggtcatattg
     6841 ctttaaataa tatagatttt aaaaattgtt ccgccacgtt aggtaaagtt ttagtaggtg
     6901 atccgcaaat gcgtggccaa ggattaggcc ctaaaataat aaagaaatta ttgaaaaagg
     6961 cttttgaaga tttgggacta cacagaattg aattaggtgt ttttgccttt aataaatcag
     7021 ctattaaaag ttacaaaaag attggattta taaaagaagg agaaaaaaga gactttagaa
     7081 aattaaatgg agaatattat aatttatggc aaatgagtat gttagaaagc gaatggtctt
     7141 gaataatttt aaacaaaaat agtcctctta ttaaaataag aggactattt ttgtttattt
     7201 agagcgtaca ataatcatac aatacaattg taaatttaat tatattaaat ttacatactc
     7261 taaatctata agtggtactt tttcttctaa taattcatta aaaaattcat ccacagcatc
     7321 atttgaatta atataacgcc agtttaaatc actcttatat cctttttgat ttgatctata
     7381 gctttgacta ttgcaataac tattaatttt atctttagga attacatttt cttttattaa
     7441 ataataaata aaatatctga aataccataa attttgtgac attacaccta ttttttcggg
     7501 ataactatcc ttagaatttt taaataggtc atcaattttt ttcaataaac tttctattct
     7561 ccaaggcttt cgtagataaa ttattgtcaa taatgacaaa gataaattat ctgtattttc
     7621 atttagtaaa ttcaaaacat tcttttttaa aagaatatct acatcatatt caacaatata
     7681 gacaagaatc tggtatgttt cttgatgata atcattttta ttataaaata ctaataaatt
     7741 tttaatctct tcgttcctat ttctaaaata ctgtttaact tgttttgaga ctactttttt
     7801 atcatccatt ttaacagcca aatagtttaa taaagataga catctatttg ttaatttaga
     7861 gtcctttagt gataaatcta atataaattg taaaatgtta aatttagtaa tattattggt
     7921 atagccaaat atcttactta ttttattttt attgattttc ttatacttga aggtattttc
     7981 tattactgga aaaatacatt ttattgcgcc tttattacct tttctttctt catctataca
     8041 taaatcaata aagtttttaa tacctataat ccaagtttct tctttactat ctgaagaata
     8101 atttttaaaa taattaaata taaagtcttt gttctgtgta tgtatgtatg gaaaatcatt
     8161 tatttcggtt ttcttatcat tgattttcaa ttcatatttc atacatagct tattaaaatc
     8221 tctgtaaaac ttgtcttttt cctcttcaaa attaaacggg aatgatatgt catctacgta
     8281 tctcgcatac ctatatcctt tattttccat ttcagaatca atatagcaca tatataattc
     8341 actaataata cgacttaaaa tattcccagt aggtattcca tgagtttgat tgtattgaca
     8401 ggaggcaatt aacttatcta atttgtttga aaacccttta tccctttctt ttttagcatt
     8461 tttcttcccc attattaccc aagggatact gtgtgtatag agagaatggt aaaaattgga
     8521 taaatcaaca tttaatatat gattcccccc gaaaagtaaa gtctttttta aattatccgt
     8581 aaatttaaaa tcaaaatctg caagtgaaaa aaatttagat gttgaaaatt tattagctag
     8641 aaaaattctt ttaaattcct ctttattttt ttgtataaaa aacatcaaat ttaaatagct
     8701 atatatatta gggattttat actctctcct tgatatatta cttttcggaa tagtaaatgt
     8761 tattggttca gtactatata cttttttatc attaaacatt ttcatagtat aaaacggatt
     8821 atcagaaata tattctgcta acttactcat gttaaatatt ttcaaataaa atgcatctga
     8881 tttaccatag ctatttaaat ctgttatatc ttgattattt tttggagtaa tatcaaaata
     8941 accatagtta attaaaaaat tagtactaaa taaatcatca tacaattttt ccaaactttt
     9001 tttacttttc ataaccttcc tcctttaatg ttccttctat ctttgcattc ctctccacta
     9061 tgatgttata atacatctaa cgaagattat atgcatatat gcaccaatcc cctcactatt
     9121 tgtggtagtg aggggatttt ttgatgcggc ttaacgtcgc ctcctattta ttgcgtttac
     9181 tactaagcca gtaagtaaaa tacgcaactg cacagccact aatgactggt gcaatgatgt
     9241 gaacgaagat cataagcata ttaagcacct ccttccgtcg aaaataccgc cggaagttaa
     9301 ggcgactctg ttattatatc atttgtgaat acgtaagcat atgcacttcc gataagttga
     9361 attatgtcag ctcaatgata aaataatata gttacttcga ttaaaataat acaatttcca
     9421 ctttttctaa agtcaaaatg tttataataa atatgagtat attattaaaa aaatttgcaa
     9481 atattgaaat gaattagtta aagatgaact aactaaaata ctattacagt ttatttagat
     9541 gcattaataa aattgtaagt gttttatact taaggaggat tttaaatgtc gaaagaaaaa
     9601 ataactagag aaaaaacatc tattaaaata cacagaactt tcaaaccatt atttgatgaa
     9661 ttagtagcag aagaacgaga cagtaaaata gattttatgg atgcgttgtt agtattttat
     9721 gctaaagaaa accatccaga gattttagaa gcctttaaaa atcaggaatt aaaaacgcaa
     9781 aaaaatgaaa aagcaaagga tctataaata aatgtagtat ttattatcat tattcatttg
     9841 tctaccaatg ttcctttata agcagtcaat aatgcataaa gaaagatacg tttaaatgaa
     9901 aatgagacaa tacatatcta aaatacaaag cgcttaatag aaataattaa tctattaagc
     9961 gctttgttta cttaatcaaa taaatcataa attaatgatt tctgaaaaag gaatccttgc
    10021 tcttttctca atttatataa attagaacac gtttgcacat aatcaaatat caaaaatgat
    10081 ttttaaaaag aagagattaa aaaaatcatt agtgaataaa cttctaactc ttcataccaa
    10141 aatagctcct tataaattta aaaaacataa aatttaacat ataaaaattt gcgatttgtt
    10201 ttattgcttt ttaaaatttt aagtatcaat taataacaca aattcatttt ctataactac
    10261 ttaaatttcg tttttggtgt ttcattttgt caaaaatggc ttattctatc agaataaaac
    10321 ttcaaaattc tttattctac tagaataaaa gggtctctga agccctttaa ataggcttta
    10381 tctataggat tggtgttcac attaataatg ccattcgtaa tttatagaat aaaaattact
    10441 taattcattt ttgtctaatg taaatgtact ataaatactg gtataaccgc atttatagtg
    10501 agagcaaatg acgaaggaat ggtaagaaca atacatcagc tatgctgatt gacacgtcag
    10561 tggcattgta tttccttatg ctcttatttt ttattctgtt tttagcataa aaaataataa
    10621 ccttttaata aaatcaatca caacatgtcc aaaaattcat tataaagaat gagtttatta
    10681 aattattagc agtagagctt tagagctctt aagttatttt tgactttttg aattacattt
    10741 acttaaggta tacattcata aaaaatggac ttctgtattt aagaagtcca tttgccaaat
    10801 taattttata atttttcttt acctactgaa tctccaaaat atatttcttt ttcatcatat
    10861 gttccaccat tcaagtaaaa atcttgaatc tcttttttta gtgacttttc tttttccaat
    10921 actgctctcg cctaaccttc aagaaattta aaaacataaa atgaacgtta actataattt
    10981 ttcaaaataa tagttaacac ttgcattgtt acatttttat attatttaat tatacaaatg
    11041 aaatacacaa tttcttttct gttacatcag ctactgatct taaaagagac attgttggaa
    11101 gtacatcccc gttttcaata atttcaatag ttgtttgagg tacttttagt ttttgagcaa
    11161 actgcatttg tgaccatcca atatcttttc gtaaactaac aacagctatt gagaattcta
    11221 atttctctcc ttgttctttg tacaatgttt ctaattctga gtcgttattt atatcttctt
    11281 gaattaattt agaaattaat tccatttaaa atcactcctt atatcaatta taattaaaaa
    11341 agaacgttat ttctatatag tatcaagaca agaagaaact cgtttcactc gtttcaaaaa
    11401 ccctcctgat ttttagagaa aaactctctt atttcatcgt ctttaataaa ataatatgcc
    11461 atctttccct ctttataaaa atcaagtaca tcatttttat ataaaagtct taaatgatgt
    11521 gaagttgaag caacactcat tttcaatatc aaagaaatat cacaaacaca caactcatct
    11581 tctttaatca aagataatat gattttcaat ttcttctcat cacaaatttt ttctaaaatg
    11641 ttaagtaatt tcttagattt atcatcttct aaaaaactta aagcattgtt aactttatcc
    11701 tcatgtacac agatcacatc acaagcattt tcataactca tataaaatca caacctaaat
    11761 taaaatataa atcctaaaat tgtttgaata gtgtcatttt caataataat aaataaacct
    11821 aaagctatat aaataacagc cataatccaa cgactaaatt tctcaacaat ttctccaact
    11881 cctggaatat tagctaattt ttgtgcagta aataccaaga agaaaattaa aattaaaaag
    11941 acaaacaaag taattaataa attagtaaca cttaatgtca caaaatacgg aacaaataaa
    12001 ccgatattat cagcaccaca acttgctatt gtaacaattg caaccgtacc aactaattta
    12061 gacaatcctt tttcattcaa ttctttttta gctctctttt ctccttcaca atcatcataa
    12121 atagctactt taattcctaa ataaatcggt attaacccta ataatcctaa tatccatttt
    12181 tcaggaacat aatttaagac aaaggcaaag aataaactta cgacaatcaa tgccacagaa
    12241 cctacatatt gaccaatata aatatctcga tattctttcc tagtctttgc tctagcaaaa
    12301 aatattaata aaattactaa taaatctact gctgtcgcaa tataaagaat agcagcagtt
    12361 acaatagtct gaatcataat tacacctcat tcaaaaatat ttttgaatgt attataactt
    12421 atgttttcaa ataatcatag atgctttatt ccaattgctt tattgacgtt gatcctcgga
    12481 acccttaaca atcccaaaac ttgtcgaatg gtcggcttaa tagctcacgc tatgccgaca
    12541 ttcgtctcca agtttagtta agggttcttc tcaacgcaca ataaattttc tcggcataaa
    12601 tgcgtcaact cttttattct tcagaaatac ttttagttaa taaagttgct ccaccaatta
    12661 acattccaat gcccaaaata aagaaaccac taaagatagc aaaaagactt cctaaagata
    12721 agaaaataga aaataaatta cgttctgttt ttttcaactt caaaactcct ttttagcaaa
    12781 ctttatcaca atagttatcg ctttacaggt ctgattttcg tcgttataat ctagttaaat
    12841 cccgtaaggg cgcacttatg cgttctaaaa agaacgcgtg caaaaaccat aatcgacctt
    12901 cgtcgatttt ttatcttagt tttaagtaat cggtgttgca gcacagatat aaaaattaat
    12961 actgcattat tagtaatcgg cgttgtggcg cagatacaaa aattaatact gcacgaattt
    13021 gaatcgacgt tgtagcgtag atataaaaat taatactaca tcaaaattta actaagattt
    13081 aagctactag ttttttgcta gtagcttttt tattttttaa aattttaaat acctttattt
    13141 tgagttttaa gggggcattt taaagattta ggggtaaatc atatagtttt atgcctaaaa
    13201 acttatataa gctcttaaaa cgcaaatatg agccaaataa atatatgcta tttccaaaag
    13261 caaaaatttg agcaaatcca gtgtcgattt tttaagacac tgcccagtta catgcaaatt
    13321 taaaaattgg cataaaaact ggtcaaccat gccggttgaa cgctatagtt cccgcagggg
    13381 caaaaagaca taaaaaaacg ctagctttta agctaacgtt tcaaatcttt taagtaataa
    13441 ttttgttttt caaaattcca aattgccgta attgaatgtg ctttttcttc ttcgtctgtt
    13501 gttttatctt cgtcacttgt attaatcaaa ttaccatctt cggcatcgtc taaatttaaa
    13561 attttatgtt tttgtttaag caaaccacca taactcaaca tacgttttcg gtataaacct
    13621 ttttctaaat catttacgat ttcttgattt ttttcgtcat tatcagttaa aaaatctgat
    13681 gacttaaccg agtattttga agtctctttg attgctataa aattatctaa attattggct
    13741 aaagatatta tgttgtttca tgttttgacg acgcatatta agtgcgtgta tttcttcaag
    13801 ttcagcttta atcgattgca ttaaatatcc ttgcatagct tcaacggttt cattcttttg
    13861 tagcagcatc gctttaaaac gttttaagac actgacaatt tcaaactcaa catcttctaa
    13921 acgataatag gtatcatggg cattattaaa tgatttcttc cctttgagta aaacactttt
    13981 aataatacga atttctctaa tttcaaaatt acttacgtaa tctttgattt gttgtggcaa
    14041 ttcttcaagc acttggaatt ttaaggcatc attattaaat tcattttgtt gatgatttgt
    14101 atgattcgaa tgattgtggt ttgttgaatt gttatatgta tcattcatat catgcgtatc
    14161 attattctta gtctctatat agtcagtatt acttaagtca gtatcattag tgtattcttt
    14221 tgatatgtca tgatgtatca aattaatatt tcttgatgta tcattatcat atttctggac
    14281 gtatgaattt gatacgtccg tgttattacg ctgttctata tcattttctt gttgtgaaat
    14341 ataatataca tcagcttctg ttacttccgg ttttttaata tataatttat taggtttatt
    14401 taaacctact cttatttgat tgaataaatc agcttgagtt aattcttttt taattttaga
    14461 taacttattt ggactactaa tatttaaaat agatttaagc ttttcattgg tataaataaa
    14521 aaatatatct ccatttttat ctacccaatt atttcttata gataaatcta aacgatctct
    14581 taatattgac catgttattt ttgcatcgtt agacaaatta acatatttag gattcgtaaa
    14641 aaacacttta ggtaattgat aaaatttttc tttttggatt tctttaatgt taaagttgga
    14701 catacaaaaa cctctttcat atttaaattt ctaaaagagg taataaaaag ctataagtaa
    14761 gtttatatgt attcgacttg cactaataat tcttagaata tataataaga atataacaaa
    14821 tatataaaca aacaaatcac ttgttttatt accgaagaaa cctgactatt cccgtagttg
    14881 ggtttctttt atatatattt ataataacac ttttaaaagc tttgttaaat tgtttatata
    14941 catttggatg tatgcatttt caaaaaagat aaagcttcgt ataatacctt tatatggtta
    15001 tgtcatataa cagcgtataa acgcttatat agcaagtata aagggtgtat aacacctata
    15061 catgtagggt tgaaagttta tacatataat cctatatagt tatacgtata accgtatata
    15121 gctatatgta taatgaatta tatgcccttg taataacaag tataatagct atataatggt
    15181 ataaatgtca tgtgtataat tatatggagg ccattatatg aaaagtatga aggaaattgc
    15241 cgatgaatta aacgtaacaa aaatgactgt ttataataat gctaaaaaag caaatgtaaa
    15301 atttcaaaaa atcgataacg taaattactt atccggtgaa gatgaaacta tagtagtaaa
    15361 ccgaataaga aaaaatcaaa ataagaatga tgattttgaa ggtgaaaaaa aagaagaagc
    15421 tgaacctaat aatgataacc tggtgaaaga tgagacaata aaacaattat ataaccaagt
    15481 agctatatat aaagaacaat caaatagaga taaagagcaa ataaatacac ttaatagact
    15541 tttagagaat cagcaaatat tggctttaga aagtaataaa aaaattcaaa aactagaaaa
    15601 tcaattagag gaagaaagac agcttaatta ttcctttgat acatcttcta atgatagaca
    15661 aaatgttaag gcgcaagaag caaagtttac cgaagaaact aaagatttta atcagcaacc
    15721 agaaaaggaa cagcccatag aggtacagca taaagatata cctgaagaga aagagaatga
    15781 aaacctcaat gaagaaccat ccacaataga cgataaacaa ttcgaagaaa tagataatga
    15841 atacgaagat aaggaagaaa aatcacctaa aaaaggtttc tggagtcggt tgtttggtaa
    15901 ttaacacaat aaaaagcgct acaagctcat gaacttgtag tgctttttta tgctagaact
    15961 tctgataatc ataacttatg tcaataaagt taaattcata taaatttcta attctatagt
    16021 ataagcatgg ttataatcat atcaaacatt tataatatat gattaattaa atgattaaaa
    16081 tttacatttg tatatcatat aatattttat ttattttttc ttttatttca tcaatactta
    16141 caatgtacca ctctgtagct tttttataag cagaatcttg aaaaataact tgtttatctt
    16201 gaaatgcgtg gtgtaaagta cgttctaata attgggcatt taaattttta acctctgcag
    16261 ataatactag gcgaactgga gcgtttaagt atgtaacttc attttcagca ttaacgatcc
    16321 tagattcaat cctatttcta gtaaatccta ttttatataa attgtttatt tttctaattt
    16381 ctggattttc acttaatgat tttaaaacat atatataccc agtggtaaac tctgcgccaa
    16441 atctttcaga gagcgactcg tcgtcaacaa tatttgttac cattctacca tgacgttctt
    16501 ttgaatataa ggaagaagct aatgattgta ataataattg cttattttct gttccatttt
    16561 cataaaccac atgcataagt gcattctctt ttccgttatc cccagtaaat ttttcactag
    16621 aaatggatac aatatataac attacaccat tttgaatgta aaatctccca gcttcaatgt
    16681 catatttttc aaaaggtata attttccttt taccattagc gatgtcttca tgaacttttt
    16741 caaatagttt ttgatattta tcaaaattag ttgcatgctt ccgcgagctt tttttacttg
    16801 ctgcattaac ggttctttta tatcttgaaa catcaagtaa atcagaaaag tcatcgaata
    16861 aattttcatc ttctaagact tcattaaggt tatttacttg attagcatta ttatctaagt
    16921 tcattctatc tgaataattc ccttttttgt ctaataaatt taattcgtca agatgtttaa
    16981 ctttatctaa acgctctttt ttctcgcgaa aaccttgaag tcttgcccat aatgcacgtt
    17041 cttctgacca ctcatcagtc ttgtttggct ctctatcatt ttctttaaca aaatttaaaa
    17101 tttcttgaaa cttaatgatt tctggatcca atttttctgc ttttttcttt tttacttgct
    17161 tagttaagtc atcgaataaa tcatctgaaa aaatatcatc taaattattc gtcattttct
    17221 tgttcaacct ctcgttcatg tttaatttca gctaatctta atagtgcaaa tgctaaatac
    17281 ctttcaccct catctttact atttctatct gggttatgcc cgttctcaga tcggaattgt
    17341 tttgctcgtt gatatgcatc tatcaattgt tcttgtgtaa attcatattt tttagatgat
    17401 atataatctt gaacaaattg tagagttgat gcattaatat cacgactgat catttcatat
    17461 gctctttgga atggattaac tgaatcaatt aagtcaatat ttaactcatc gatattaatg
    17521 aacctatcgg ccattttaat aatttcacga tttccatctt cagacgaagt cttagaccct
    17581 gctgaaacat taatatcagc aactgcatgt tgacgaactg tttctaattc ctcagtagtc
    17641 aactcagggt agcgttcaat aattacacgc ggaattaatg ttttgttaat cgtttttgca
    17701 tcagagcctg acacgatagc gttttgaata tcttgacttt gtaaaatact ggacttcaag
    17761 tcagacatgt cgtcttcaat gatttttttc gttcgttctg ttgtatttga tgggtttaaa
    17821 ccttgaattg acaattcatt atcgctacta tacttttttt catctttagg cttaaatttg
    17881 tacactgggg ccaacacttg ttccattagt aaagaagctg aaatcgcctt cataatactg
    17941 tttaccgcat acaaaacttc gtcatcttgt gcatctggtt gagcaattaa atttgtaaat
    18001 tgagcatgat ttttattagg gctatcacgt gttactcgtc cgatgatttg gataacttct
    18061 gttaaagagt tacgataacc aattgtcaaa gcgtattctg caaagggcca gtcaaaacct
    18121 tcttttgcca taccaagcgc aataataata tctaatttat ctagatcttc aatatcatca
    18181 gatatatttg ataaataaga agtaactttt tcacgcgatt cttcattaac caagtcagca
    18241 actaatagat gttttccatc gggacgacta atttcataga taccatcttt attttgaaga
    18301 acttcgccgc catcagaaat tatatccata atttgatcga cttcattata tttatcttta
    18361 gttgactcac cagaattaac attaggtata tgaatgattg atttctttgt aacatctata
    18421 acttcgtcta aagcttctgt atagcgacca cgataaaaac tatagcccat agcaaaagac
    18481 tttaaatatt gatatccttc tagctgctca taatatgtgt aggtcacttt atcaaattta
    18541 tcctcatcag atggctccaa tatttgagta gaatccccgc ggaaataaga tcctgtcatt
    18601 gctaaaatat gtgcggttga attattcata atactccgca atgcatttcc aagaacagag
    18661 ctatcttctc gactgacatg gtgaaattca tcaatagcaa gaagtgaatt gtcgaaatat
    18721 gaatcatcta attcttcaaa agcaaagcgt aaagttgcat gggtaaggat tataatatca
    18781 tcatttggat tattagaatt cataaaatta acgaaatgtt taacttttga ttttctacct
    18841 acagtagtag ttaaattatt attgggattc acatgccaat cccaataata accgtacttt
    18901 tgaagatcag tatttctaaa tgattttccg attgaacgct ctggaacggc aataataact
    18961 tttttgatat cttgattttt taatttatcc aatccaacaa acattaaagc acgcgattta
    19021 ccagatgctg gtggagcctt aactaataaa taattagaat tacgcttttc ataaacgcga
    19081 gcttgcatat ctcgcatacc gagtgagttt gttgattttg attgtgtttc atcaaaattt
    19141 atttcgaata ggttgtctgt catgattcta atctcctttt gtcatatttt gatataattc
    19201 gagcagtatt gacagtcgtt cttcatcgct attaaatggc ttttgtcggt aagctctatc
    19261 aataatccca tctagttctt cgtgcttttg tcgtaaactt ttaggcatag tttttgtatt
    19321 gtataactca gctaaagaac ctccttcata ctctcttatg tctaaaatat caagcataac
    19381 acgtgctatt tcattttttc tttgtgttga taaattttga actggaaaag tattatagac
    19441 taaccctgat gaatatcgat acctagtttc aagttttcct ccaattgaac gaagccatac
    19501 catatgcatc cgtgactgta aaaggcctaa aagccatatt ggagcattat agatcactat
    19561 cgcagaatct gaaataacag tattattatt aacatacccc attggaacat aatcacgaga
    19621 ttctgaggaa acgcgtggaa ttataatttg tttaattcca gtatcactac cattatgttt
    19681 ttttctataa acctcaaaat atttcacacg ttcaccattt tgtacaaagc gataaggtac
    19741 ttttccagct tccctagttg aagttgcttt actctccaat ctcatttttc tcactttatt
    19801 aatacgtttt tcaatttctg gtatatctga taactcatta tattgagagt gtgtttcaaa
    19861 ccaaagcgta taacgaaaaa tatcgttgat aaactcatga gccccaatat aatatttaat
    19921 atatttacga aggacgatat tgtttgtata tttatcaaac aactcatttt tttcttcttc
    19981 agaaaatatc aaattaccac catcacgcgg catacttcca aatacagctt ttggaaatcc
    20041 agaaatgctt tcattttgtg atgtcacaat gatattgggc gctattgtta gataaggatt
    20101 gatcaccgtt gccatttgtt tcccgtgttc agaatataaa taactaattg tttcacgatc
    20161 cttggattct aaggcaatga tagagactgt cactccagca ttacttttag cattgttttt
    20221 ccatttaaat gatgtatatg caaatgataa cttactatac tcgaataatt taggccaaat
    20281 taaagaaact tgttcgccct gattaataga attagtagaa acaaatgcta ctctattatt
    20341 tgaattgcga ataaattgag cccctttata aaaccatcca gttataaaat ctactttttt
    20401 actattaatt tcatcgccta acacaaatga taaatcagat ttttgttgtt tgttttgtaa
    20461 tttggcaccc aaatacggcg gattccccat aatataaact tcatcattag gtttatgtga
    20521 cacaacttca ttccaatcaa tacgtaatga attaccacga ataatatttc cagcatcctt
    20581 taaaggcaaa agagctggat ttgctgacgg tattactttt accatttctt cattcatttg
    20641 atgttcggca atccacaatg atatttttgc aacttcgtgt gcaaagtcat caatttcaat
    20701 accgttaaat tgacttaaac ggattgatga cattggaatt tgcccagatt gttcgatttc
    20761 ttgttctagt aatataattt tgatttcaag tctgcgtatt tctttataag tgatgattaa
    20821 aaagtttccc gacccgctag caggatctaa aaacttaatg tttgaaatac gagtatgtaa
    20881 cttacctaat gatttcaata tagacctttt attttcttga cgtgttttat ttgtaatgtt
    20941 tttatttgta atttttttat aactcatata taatttttca aattcgtttt ttaattcgtc
    21001 aagaaatagt ggtttaataa ctttcataat gttcgagaca cttgtatagt gcatacctga
    21061 actatgacgg ttttctggag aagcaactga ttgaatcata gaacccaaga tatctggatt
    21121 aatttcattc caattaagta attctcctgc ttcaattaac aattttctgg aaacgttggt
    21181 aaaagttaat ggtatatgac cttcgtcaaa aagtttgccg ttaacatacg gaaacacctt
    21241 tagccaattg aactttccaa aacggtttga ttcattgata tttagaattt caaataaatc
    21301 tttgatgaca tcgttaaagt tactaccatc ttcatttgtt cgtgtcttaa gtgtattggt
    21361 aaaaacccct ttttccataa tacctgtgtc ttcagcaaat aataagaaaa gtacacgaat
    21421 taaaaataag ttaaaactgt gctcgtcaga attgggatta tcttttgcta atacatcata
    21481 aatttttgca aatcgttcag cagcctttcg atcagccgga tgttcagatt gataatccgc
    21541 cttttcaata ccattccatg ctaaaaagaa atcagctttt gaaggtaatt cttcaaatgg
    21601 aatgttgagt gtgtcacgtg tttgaatatc taatgctgca atgtttatat aatcgtttgc
    21661 tattatgtag cgtggcttag atttttggtc ttgaatttta tgttcgattg catctattgc
    21721 tgatacaacc tctcccgtag tttctgtata atgtagtttg tttttgataa caaaaggttc
    21781 tcctttatca aatttcgctt tcgcacgagt gattgatgtt ttaggaatat catacaacat
    21841 caaaaagttc gttataaatt ctttttcagt aagattattg attaaatctt taataccatt
    21901 ttcaatttct gtgatgctca aagtcttttt tttaaccatt tgcctatccc tttccttttt
    21961 ttggtaaata aactattttt aaaccattta acttaatata ctatctagtt catacttgta
    22021 ttatcgataa atatttatta ttttaacttt tatataatgt aaattgattt cttatttaaa
    22081 ttcttgttta agatacatct aatcgcttga aatatgaggt gagcaacttt ttgacatata
    22141 tttaatctta ggcttgatta ttacatattt gtttataact ataaatatta ttatattaca
    22201 tagtagatat ttataagtat ataactttat aagattaact catattgtta tgtagagtgt
    22261 tattttccaa tcatatttaa cacctttttt gctaagttta aaatataatt ctatcactat
    22321 taatataaaa acgttcgtta ttgaggcaaa atttttaaac catctctttt aaaagagatg
    22381 gtttttttta acatgctgtt gtttcttatt aggaaatttt gtttctttaa gttgttatat
    22441 aagaaatata ccttatttaa aatttctagt aacattaaat ataagaggtg ttattaatga
    22501 ttaaagtatc taaaataatc aaacaagaac gtttaagacg gggaatgact caagaagcac
    22561 tagctgaata tcttaataca acaaaaacaa ctatttctaa gtgggaaaat gcaacacttt
    22621 accctgatat tacaatgcta ccaaaactag caaaagtgtt taatatttca gtggatgatc
    22681 tgcttaacta tagcattact atgacagatg aagaaattaa ggaagtatct atatcattat
    22741 ctaagatgat caataaaact agttatgaag aatatgtatc aactgtacgt gattactata
    22801 ttagtcattg taatgaattt aagttcctca actcattgtt agggatttta gcaaataata
    22861 ttttatattg tgagaatgaa acacaagtta aagaaacaac aaaattagct tatgaaataa
    22921 ttaaaacaac agaggataac tcagaatcta tttacttgaa aagacatgcg caaggatatc
    22981 aaacattaat gtatatgttt gaaggtgact attcatcagt tatagataat acacctgatt
    23041 ttgatttaaa aattggtgaa aatgctatct taactttagc ttatttacaa aaaggaaata
    23101 tttcaaaagc caaaaatgta attcagacag atatgtatca atctctcatg atttatatgc
    23161 atgactttgc tttaatgctt acttatgact tatattctat tccaatagaa tatttagaat
    23221 ctaagcttca ttatataaac aaagctttta atgtggatta tttgtaccct cacttaagta
    23281 tgaattgcta ttataaattt gcattatatt acgttaaaaa tgatgataat aaagccattc
    23341 aatatttgaa gcagtattgt caatgctttg attatttgac ccaagatttt gtttatcaaa
    23401 aagatgaatt ttttaatgaa attgacgaat ggctaaacac tttaccatta gaaaatcgct
    23461 taccagcaaa ttataaaaat actttgaata cagcaatctc taggatttta gaaaatgagg
    23521 attttatgac cttggataat tttacatcta tagaagaaga tgtgtacaga atatataatg
    23581 aaggaatgaa aaataattga tacatagaaa aaagctaata gtattactat tattaagttt
    23641 cactttgctt tcagcttgtg attattcaaa gtcagaaaat agaaatggct ttttctatca
    23701 tacttttgca gtaccgatgg atcatctttt acattggtta ggctctatat ttgataataa
    23761 ttatggatta gcaatcatta caatcgtttt aatcgttaga attatcatgt taccgttcat
    23821 gttagctcaa tctaaaaatg ggcatttgat gcgtaagaaa atggaaattg tgaaacctga
    23881 attaactaag atacaagaga aaataaaaaa agcgaatacg caagaagaaa aaatgacagc
    23941 gaatcaagaa atgatggaca aatataaaga gtacaatatg aatcctatga agacaatgct
    24001 aggatgcttg ccattactta tacaaatgcc tgttttattt ggactgtacg tttcattaaa
    24061 atggccttcg agtggtggtt taactgaaca tactgatttt ctttggttta atttaacaca
    24121 tcctgatatt tggattacta taattgctgg tattttgtac attatacaac ccttaataaa
    24181 tatggaaaat atgcctaaag aacaacgctc aatgggttat atcatggcaa ttatttctcc
    24241 tatattcatc atatatattt ccattacatc agcttctgct ttaggactgt actggacgat
    24301 taatgctgca ttcttaatta tccaaatgtt cttctcaaat agatattatt cgaggttagc
    24361 tacaaaagaa gctaatcaat tgaaaagaaa agttgaaaat aataaacttg ctactaaata
    24421 tacgtagtct taaaacaatt attacataga aaaagagtct ctattcggct cttttttctt
    24481 gtgtttttat taaaatctca atttaataat atattatgag atttgagacg ctgtgaggac
    24541 ctctcatttg cattgacaat caatttgtca agataatccc ttatcatgtt taattctata
    24601 aactgtctgt cttgtaatat tgacttcttt cgcaatttta ctaattgctt ggccttcttc
    24661 taacatttcg acaacacgat aatagataat acgcttttgg ggatctttcg cgtttggtga
    24721 ataaagtaaa gggcgtcctt tatatatccc tttgtctttc gcaacctgaa tcccttgagc
    24781 ttgtcgacgt ttactttcat ttctctcctg ttctgaaacc atggctaaaa tttgaatgat
    24841 taaatctttc ataaacttat ctaataatgg attaccaatc acttcattca tcataggtaa
    24901 gcttgtaatc attaactgaa catctttttc ttttaaataa ttcacagttt caataatctc
    24961 atcatagtta cgtcctaaac gatctattga ctccacaacg aaccgatctc ccattcttac
    25021 aaaattaagg gcttcttgaa atacaggtct attttccact gatttacctg attgcttctc
    25081 tgtaaagatt ttctctacac caaacgtctt taaattatcc aattgtcttt ctaagttttg
    25141 atctatcgaa gatactctcg catagcctat aatcatttat tttcactcca tatttaatat
    25201 acaccctaat catacatgta ggttgctgta atttcaacaa tctactttgt gtgattaggg
    25261 tgtactcaaa gtgtacatat ctataattaa aacttactaa aaataaatat agattttttc
    25321 tttgtaacag aaccgttcat ttgaaggtca tctctatcat aaatatttaa attaactaaa
    25381 aattttaatc atctgtttaa tacttatgaa atgtatttac ggtaggtatt aaaattgatg
    25441 aaatttatat aagtgctttc aatgaaaatg accgtttatt tatcctcctg atttaaggag
    25501 ttagaaattt cgaaaacgat tctttccatt tgttgcattt gttccatgtc cagatattta
    25561 atacattata gaaaaccaaa gaccatcact aattaaacgc agttttaaaa tttcatcagg
    25621 attgccttcg tctttaaaat gggcctcttt taatataagt tctactcttt catacctaac
    25681 tctttcaatg ccatcgtttc atacgccgat tctcctgatt ccatagcaaa ctcagcgata
    25741 aacattggga atactgcatt gagttctatt atatgctctt tcattcgtgg ccatatttca
    25801 cctccagctt taccgtatgc tgttataagt tgctctaatc cttcgtcgtc gaatacacga
    25861 tggtgtccta taaagtccat tgatgggtcg gagtgggttg cttcagtcca gtctatgaga
    25921 cctgtgacgt ttgcttggtt atctaccatt atatgtcctg gatgtaagtc cccatgtatc
    25981 atggtcgcat gtcgaggcca tagttcgtcg ttttctaacc attgtttcca tctgttccat
    26041 aattcatctg atacgccata agtttcttta actttattca ttcttctttg aaagtcattt
    26101 tttatttctt gtatggtttt gatatttata tgctgaacgt taatgttttc ttctggtatg
    26161 ttgtgtaaat ctacgagtgt ttcagctaat gtgttaataa agttttctgg taatggtttg
    26221 tgttcaattt cccatacata attttgtatt tctggatcta ttgtggctgc gggtttacct
    26281 gtaagttttg ggtaagcaat aaggtctttt gcatgtactt tccacttcgg tatttcaaat
    26341 gaaacattct tctgtaagaa gtctaccgtt tgtttttcgg gttttgttcg tttataaacg
    26401 tcaggtcttc ttggtagtct caaaacccat tctactccat gtttatcttt tccaaatgca
    26461 acttgaaaat caagtcccga ttcattcaat gagattgttt gaggttggat gtgtaattga
    26521 tatttttctg cacatttaat aatttcatta tgtcgagtca ttttattgga tccttatttc
    26581 tattatattt ttcttgtgaa cgtatttttt atattgatat aaatgaacct aatattcaat
    26641 ataaattagt gtatagtttt taagttatat catgaataga ttgtcctgtt aattcccatt
    26701 ttttatctga tacatgttta acaaaccttg tatcatgtga tgtaaacaaa atgattccag
    26761 gatatttatt cataaacatt tctaatgctt ctaatgtttt aatatctaaa aaattagttg
    26821 gttcatccaa aattaacata ttcgctttcg ttgaaaataa tactgctaac gataatttcg
    26881 ttctttcccc accactcaaa acattacaag aacgatcaag tgcttcattt aaacccaagt
    26941 tatttaaaat agctctactg aatgattctg atgaatccgt ttcatccatt aaatattgca
    27001 ataatgaaac gtcacgcatg tcttcataag caagttgacg atagtatgcc atttgcactt
    27061 taggagaaca atcaattccc tctatttggt ggtaaatagc ttcaagtaaa gttgtcttac
    27121 ctacaccatt tgcacctacg agcgctatat ttttgccata tggtatttgg aatcgtactt
    27181 gtgttagcag tttttgactt cctttaacca atgttagatt ttgtgcaatg attggataat
    27241 tattatggat atcataaatt ttattttgtg ggaaattgaa ttcatgataa ctttgtggtt
    27301 tttcaacttc ttccaaatgt tccattcttt tttcaatatg cttagcttgt ttttgagcag
    27361 ccttctcaac cgtgcctttt tgttttgatg cacttaaacg atctggtgct atacttttgt
    27421 ttttttgttt tgatgatgct tgtgccattt gttgcgcttg atttcgttta gctttactgg
    27481 cttgggacaa tctttgtttt tcacttatat actgttcata ttgacgttgt tgttctaact
    27541 gttcttgttc atattgattt tgatactgtg tgtaattacc tttaaacact cttattgtgc
    27601 catcttcttg tatatcccaa attgtgtcag caatttggtc tataagtgct ctatcatgac
    27661 ttactataat taaagtacca tagtaatatt ttaaaatatt tttcagataa tctttaccaa
    27721 ttttatccaa gtgatttgta ggttcatcta aaagtaatat cggactataa tttgatatga
    27781 cattagctaa tttatatttt gctttttcac caccactcat actgtcggtt gtatgcatag
    27841 gtatatggag ttcactcatt aaactaccgt ctaacgtgtt aaaatcattt tccacatcca
    27901 tgttaagctg ttcaaaatat tgaatttcgc caaccgtttc aactgttcca gattctggtt
    27961 ttgttttttg agcaatcata tttagtaatg tggttttgcc agtaccatta cccccaataa
    28021 gtgctatttt ttcaaattgg taagcgtaaa gatgaccgat gttaagtgat cgtaaatctg
    28081 tcaatttatg attgatttgg ttaaatttaa ttgtatattg ttccatattt atacactcct
    28141 tgtatattta aaggacgtgt acgcaaaaaa atgagacagg ctactcccgt ctcattcaca
    28201 ttacactttc aaacacttaa aaatttatta tgatgtttta gtatttgaaa gcgtttatta
    28261 ttcctatcga aggataaaaa atgacataag tcatcctatg agtgtgtatt gcggacaagc
    28321 ctttttgaat taatttagtt ttgtattcaa aaaggtttat ttgagtctca tagaagctgt
    28381 cattttaatc ctccttcatc gataaaatag taatgttaat ataacatttt gttggttttt
    28441 ttacaatata tctctaagta gaaagattat ataacaaaag tataaaaata caattttaga
    28501 ttgattatac atctccttaa tttagagtgt actcataaaa tacggttctg ttgcaaagtt
    28561 gaatttataa tataatttta acaaaaagga gtcttctgta tgaactattt cagatataaa
    28621 caatttaaca aggatgttat cactgtagcc gttggctact atctaagata tgcattgagc
    28681 tatcgtgata tatctgaaat attaagagaa cgtggtgtaa acgttcatca ttcaacgatc
    28741 taccgttggg ttcaagaata tgccccaatt ttatatcaaa tttggaagaa aaagcataaa
    28801 aaagcttatt acaaatggcg tattgatgag acgtatatca aaataaaagg aaaatggagc
    28861 tatttatatc gtgccattga tacagaggga catacattag atatttggtt gcgtaagcaa
    28921 cgagataatc attcagcata tgcgtttatc aaacgtctca ttaaacaatt tggtaagcct
    28981 caaaaggtag ttacagatca ggcaccttca acgaaggcag caatggctaa aataattaaa
    29041 gcttttaaac ttaaacctga ctgtcattgt acatcgaaat atctgaataa cctcattgag
    29101 caagatcacc gtcatattaa agtaagaaag acaagatatc aaagtatcaa tacggcaaag
    29161 aatactttaa aaggtattga atgtatttac gctctatata aaaagaaccg taggtctctt
    29221 cagatctacg gattttcccc atgccacgaa attagcatca tgctagcaag ttaaacgaac
    29281 actgacatga tatattagtg attagctata ttttttactt tgcaacagaa ccgtttttta
    29341 cattgtgtaa taaacttaaa gaacaagaca cacgtaaact agaaatacct tttgatgaat
    29401 taaaacatct tagtaattat tactcacgat cacaagcgcg ttttataaaa gatttagaac
    29461 atgtatacga taaaatgttg catttaactt atacagaacg taacgagcaa tcatttgaaa
    29521 aatttgtatt gttcacttct tataaagttg atataaaaaa acaatacctg tctattagtg
    29581 ttaataatga tttgaagcat atactcaatt ca
//
