LOCUS       NZ_CP014109             7518 bp    DNA     linear   CON 16-JUN-2024
DEFINITION  Escherichia coli strain FDAARGOS_144 plasmid unnamed3.
ACCESSION   NZ_CP014109
VERSION     NZ_CP014109.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03996288
            Assembly: GCF_002944935.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Campos,J., Goldberg,B., Tallon,L., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J.,
            Nadendla,S., Nandy,P., Hobson,J. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7518)
  AUTHORS   Campos,J., Goldberg,B., Tallon,L., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J.,
            Nadendla,S., Nandy,P., Hobson,J. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2017) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP3 v. Oct 2014
            Genome Coverage                   :: 371.832x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002944935.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 02:58:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,927
            CDSs (total)                      :: 4,812
            Genes (coding)                    :: 4,650
            CDSs (with protein)               :: 4,650
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 63 of 162
            Pseudo Genes (incomplete)         :: 113 of 162
            Pseudo Genes (internal stop)      :: 28 of 162
            Pseudo Genes (multiple problems)  :: 37 of 162
            Pseudo Genes (short protein)      :: 1 of 162
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP014109.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_144"
                     /isolation_source="Urine"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_144"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: DC"
                     /collection_date="2014-03-21"
                     /collected_by="Children's National Hospital"
     gene            <1..315
                     /gene="mobA"
                     /locus_tag="AL502_RS00005"
                     /old_locus_tag="AL502_26035"
     CDS             <1..315
                     /gene="mobA"
                     /locus_tag="AL502_RS00005"
                     /old_locus_tag="AL502_26035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_023909671.1"
                     /translation="RSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRAAAL
                     GKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRLMAD
                     "
                     /name="mobA"
     gene            complement(550..930)
                     /gene="lsoB"
                     /locus_tag="AL502_RS00015"
                     /old_locus_tag="AL502_26045"
     CDS             complement(550..930)
                     /gene="lsoB"
                     /locus_tag="AL502_RS00015"
                     /old_locus_tag="AL502_26045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin LsoB"
                     /protein_id="WP_000710826.1"
                     /translation="MKKDKKYQIEAIKNKDKTLFIVYATDIYSPSEFFSKIESDLKKKK
                     SKGDVFFDLIIPNGGKKDRYVYTSFNGEKFSSYTLNKVTKTDEYNDLSELSASFFKKNF
                     DKINVNLLSKATSFALKKGIPI"
                     /name="lsoB"
     gene            complement(927..1967)
                     /gene="lsoA"
                     /locus_tag="AL502_RS00020"
                     /old_locus_tag="AL502_26050"
     CDS             complement(927..1967)
                     /gene="lsoA"
                     /locus_tag="AL502_RS00020"
                     /old_locus_tag="AL502_26050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA
                     endoribonuclease LsoA"
                     /protein_id="WP_000068433.1"
                     /translation="MAQNPFKALNINIDKIESALTQNGVTNYSSNVKNERETHISGTYK
                     GIDFLIKLMPSGGNTTIGRASGQNNTYFDEIALIIKENCLYSDTKNFEYTIPKFSDDDR
                     ANLFEFLSEEGITITEDNNNDPNCKHQYIMTTSNGDRVRAKIYKRGSIQFQGKYLQIAS
                     LINDFMCSILNMKEIVEQKNKEFNVDIKKETIESELHSKLPKSIDKIHEDIKKQLSCSL
                     IMKKIDVEMEDYSTYCFSALRAIEGFIYQILNDVCNPSSSKNLGEYFTENKPKYIIREI
                     HQETINGEIAEVLCECYTYWHENRHGLFHMKPGIADTKTINKLESIAIIDTVCQLIDGG
                     VARLKL"
                     /name="lsoA"
     gene            complement(2232..2345)
                     /locus_tag="AL502_RS26695"
     CDS             complement(2232..2345)
                     /locus_tag="AL502_RS26695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001459398.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLSPLAAAIGN"
                     /name="hypothetical protein"
     gene            complement(2924..5929)
                     /locus_tag="AL502_RS00025"
                     /old_locus_tag="AL502_26055"
     CDS             complement(2924..5929)
                     /locus_tag="AL502_RS00025"
                     /old_locus_tag="AL502_26055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143760.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            6093..6650
                     /locus_tag="AL502_RS00030"
                     /old_locus_tag="AL502_26060"
     CDS             6093..6650
                     /locus_tag="AL502_RS00030"
                     /old_locus_tag="AL502_26060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            6833..>7518
                     /gene="bla"
                     /locus_tag="AL502_RS00035"
                     /old_locus_tag="AL502_26065"
     CDS             6833..>7518
                     /gene="bla"
                     /locus_tag="AL502_RS00035"
                     /old_locus_tag="AL502_26065"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /GO_function="GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /GO_process="GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]; GO:0046677 - response to antibiotic
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="WP_104731911.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWF"
                     /name="bla"
     ARG             6834..7516
                     /name="TEM-163"
                     /gene="TEM-163"
                     /gene_name="TEM beta-lactamase"
                     /tool="rgi"
                     /drug_class="monobactam; cephalosporin; penam; penem"
                     /identity="100.0"
                     /coverage="79.72"
     Typing          complement(2274..2803)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.972"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          2..312
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.038"
                     /coverage="97.0"
                     /evalue="4.66e-60"
ORIGIN
        1 cgaagtggaa gtaatacgcg caggcgggct atcagtcgcc ctgttcgtct gacggcagaa
       61 gaagaccagg aaatcagaaa aagggctgct gaatgcggca agaccgtttc tggtttttta
      121 cgggcggcag ctctcggtaa gaaagttaac tcactgactg atgaccgggt gctgaaagaa
      181 gttatgcgac tgggggcgtt gcagaaaaaa ctctttatcg acggcaagcg tgtcggggac
      241 agagagtatg cggaggtgct gatcgctatt acggagtatc accgtgccct gttatccagg
      301 cttatggcag attaacttcc cggagagaaa ctgtcgaaaa cagacggtat gaacgccgta
      361 agcccccaaa ccgatcgcca ttcactttca tgcatagcta tgcagtgagc tgaaagcgat
      421 cctgacgcat ttttccggtt taccccgggg aaaacatctc tttttgcggt gtctgcgtca
      481 gaatcgcgtt cagcgcgttt tggcggtgcg cgtaatgaga cgttatggta aatgtcttct
      541 ggcttgatat tatattggaa tgcctttttt caaagcaaat gatgtggctt tggatagaag
      601 gtttacgttg atcttatcaa agtttttttt aaagaacgaa gccgagagct cagataaatc
      661 attatattca tcagttttcg taactttgtt taatgtgtaa cttgaaaact tctcgccatt
      721 aaatgacgta tagacgtaac gatctttttt tccaccgtta ggaattatta aatcaaaaaa
      781 aacatcaccc ttgcttttct ttttcttcaa gtcggattcg atttttgaga aaaattcgct
      841 cgggctataa atatcagtag catagacaat aaataaagtt ttatctttat tttttattgc
      901 ttctatttga tattttttat cttttttcat aatttcaacc tagctacgcc accatctatt
      961 aattggcaaa cggtatcgat gattgcgatt gattctaatt tgttaattgt cttcgtgtca
     1021 gctattcctg gtttcatatg aaacaaacca tgcctgttct catgccagta agtgtagcat
     1081 tcacacaaaa cttccgctat ttcaccattt atagtttctt ggtgtatttc tctgattata
     1141 tatttgggtt tgttttcagt gaagtattcg ccaaggttct ttgatgatga tggattgcaa
     1201 acatcattaa gtatttgata tataaagcct tctatggctc ttaatgcaga gaagcagtat
     1261 gttgagtaat cttccatttc gacatctatt tttttcatta ttagcgagca tgatagctgt
     1321 tttttgatat cttcatggat tttatcgatg ctttttggta gtttgctatg caactcggac
     1381 tcaatagttt ctttttttat gtcaacatta aattctttat ttttttgttc gacaatctct
     1441 ttcatgttta gtattgagca catgaaatcg ttaatcaaac tcgcgatttg aaggtatttt
     1501 ccttggaatt gaatagagcc gcgcttgtaa atttttgccc tgaccctgtc accattgctg
     1561 gtggtcataa tatattggtg tttacaatta ggatcgttat tattatcttc tgttattgtt
     1621 atcccctctt cagaaagaaa ttcaaataga tttgccctgt catcatcact gaattttgga
     1681 atggtgtatt caaagttctt tgtgtctgaa tacaaacagt tttcttttat aatcaaggcg
     1741 atttcatcaa agtaagtgtt attttgccca gacgctcttc cgatagtggt atttccgcct
     1801 gatggcatta gttttattaa gaagtctatt cctttatatg tgccagatat gtgagtttct
     1861 ctttcgtttt ttacattaga ggaatagttt gtgacgccat tctgcgtcag agcagactca
     1921 atcttgtcaa tattgatatt tagtgcttta aacgggttct gtgccattgg gtcaatccgt
     1981 tgtttttttt gaatatgtac agatcttgtt tttttgtcaa cggaatagct gttcgttgac
     2041 ttgatagacc gattgattca tcatctcata aataaagaaa aaccaccgct accaacggtg
     2101 gttttctcaa ggttcgctga gctaccaact ctttgaacca aggtaactgg cttggaggag
     2161 cgcagtcacc aaaatctgtt ctttcagttt agccttaaca ggtgcataac ttcaagacaa
     2221 actcctctaa atcagttacc aatggctgct gccagtggcg ataagtcgtg tcttaccggg
     2281 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2341 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacca acagcgtgag
     2401 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2461 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2521 agtcctgtcg ggtttcgcca cctctggctt gagcgtcgat ttttgtgatg ctcgtcaggg
     2581 gggcggagcc tatggaaaaa cgcctgcggc gttggcttct tccggtgctt tgctttttgc
     2641 tcacatgttc tttccggctt tatcccctga ttctgtggat aaccgtatta ccgcttttga
     2701 gtgagctgac accgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga
     2761 agcggaagag cgccttatgt gacattttct ccttacgctc tgttgtgccg ttcggcatcc
     2821 tgtcctgaga gttatctctc tgtgctattt tctacttcaa agcgtgtctg gatgctgttc
     2881 tggagttctt ggggtctgac gctcagtgga acgaaaactc acgttaagca acgttttctg
     2941 cctctgacgc ctcttttaat ggtctcagat gtcctttggt caccagttct gccagcgtga
     3001 aggaataatg gccgagcata ttgatatgtc cgtggcaaag cggggagagg cgtgcgatat
     3061 cttcatcatt cagtgtttca ccctgcgccc ggagatgatc cagggctgcc tgcatataaa
     3121 tagtgttcca taacacgacg gcgttagtga ccagccccag tgtgcccagt tgatcttcct
     3181 gaccgtcggt atatcgtttt cttatctcac ctttttgacc gtgacagatg gctctggcaa
     3241 cggcatggcg actttctccc cgattaagct gggtcagaat gcgccggcgg taatcttcat
     3301 catcaatata attaagcaga tacagcgttt tgttgatgcg ccccacttca atgattgcct
     3361 gagtcagtcc ggaaggacgt tcacttttca gcaatgaacg gaccagcact gaaacctgta
     3421 ctttgcccag cttcagggag ccagcggtcc ggatcatttc gtcccactga aggactattt
     3481 ttcggggatc tgattgccct ctggcaatat cattcagcac gccatagtcg gcatcatggt
     3541 ccattcgcca gaaaaccgaa gcaccggcat cagccaggcg tggagaaaac tggtatccca
     3601 gcagccagaa aaggccaaag acaagttcgc tggcacctgc tgtatcggtc ataatttcgg
     3661 ttggattcag cccggtctcc tgttccagaa gaccttccag cacaaagata gagtccctca
     3721 gcgtccccgg tataacgatg ccatgaaagc cggaatactg atcggacaca aagttgtacc
     3781 aggtgatccc tctgttatta ccaaagtatt tgcggttcgg tccggcattg attgttctga
     3841 ctggcgtaac aaagcgcatt ccatctgcag atgccacttc tcctccaccc catatctgtg
     3901 ccagtggcag cgttgcctga aaatcaacca gtctggcatt agcgctggtg atagtttcag
     3961 cccgcagata gttcgctttt gtccagttca gccggtgtcg ggtcagtgca ggaacatttg
     4021 atctgatcag tggttccaga ccgatattgc aggcttcagc catcagcacg gcgctgatgc
     4081 tgacgggcag atcatcaact ctggcactgg cttcactagc atggaaaaac tcatcagcaa
     4141 atccggtatg ggcgttaatt tcgagcagca actccgttaa atccaccgga gggagtagat
     4201 cactgatcat tttgctcagt cgtttcagac tgtccggctc atcaagactg gcgaggggag
     4261 aaattgtcaa ccggggcttc gggccagaaa catcgagttc gacagcctca ttttcgcaaa
     4321 gacgtgcagc aacctgtctg taacgactat caagctgatg acccagagat tttattgctt
     4381 cctgcgggtc tgtcgggtgc cccaaagaac gataaacctt aatccggttt gcctgccagt
     4441 cagcaccctg tagtaatctt gcacgaggat ctccccaccg gttactgccg gtaacgtaga
     4501 catccctccg cctcagacta tcctgcagtt tactgagaaa gcagagcgtg tatcccctgc
     4561 gggtgatatg tttttccttg ttaatcacca gccgtttcca tgaccgactg ataatttccg
     4621 ttggtgcgtc gtcaaaaaac tgccgccgtg agctgaactc ccggctgagg tagtcacagg
     4681 cattcagagt ggtaaccccg gcaggtgcgg atgaaaattt aacggtattc agcagatggg
     4741 gcaggaaacg acgaacgcgc ccgtactgct ccaccatttc ttcatgaaaa ttatcgtctg
     4801 agggccgggc aatttcacgg acaagcgtga tgatttcagc cagcttttgc cttgggatgt
     4861 agctgaacac ctcagcacga atcgattcgt ccggtgtttc ttctttcagc aggtacgaac
     4921 atgcgctggc gagcgccaat gcagatttat ccagatcctt cagcgagcgg agccgttttt
     4981 tctgcccaat ctttctggcg tcacggatga taacggccag catggcgtcc agaacgtcca
     5041 atgcatcatc cagcgccagc gtttcccatg caaggacaaa ggcaaccaga accgccatcc
     5101 ttttctgcgg tgacatcctg gcaatattga acaccgaagt cataccagca taacgtgcga
     5161 gatttttcag gcgcacagcc gggagtgtac tcaggttttc agcatgcagg ccaaaatcgt
     5221 tcagagtttt ccagcgttca attgcttcat taaacgccgg accactgatg gtcacagggc
     5281 cctttttcag tgattccagt aaagacaggc ggctgcaatc agttggcccc agcagcatct
     5341 ccagctgtga acgctgttcg gctgacggta tcagtgccag tttgttccac aggcgcaacg
     5401 tcgccttttc ccttacctct gaaatcaacc gggtcagcgt agtggctccg gggagaataa
     5461 tacgatgttg cataagccac cctgtcgcca gatcgaaaag caggccagga cgttcgttgc
     5521 ttatccagct ccgggtatat aaaagacggg taaggcgaaa tgtccagggc caggcaaatt
     5581 cacgatactg atagtgctga cgtatcagcg ctgcatgctc acggcgggta ttttccctct
     5641 gaccgtattc tgcaagaacg gtgatatcac gaatcccgag ctgtctggcg gtaaaatgcc
     5701 ggacgccgga aggaatatga ttcatatcgg tgaggaaggt gcccagaaaa cggacacatc
     5761 caatttgcag ggcaatgccc agacggttgt gatcacctct gctttttccg ataaattcct
     5821 tgtctgcttc atcaaggtga aaatatcgtg ccagctgaag ctcatccggt tcaccggtga
     5881 atctgccata gctttcagtc tgctcagtgg tcagaaagtc aacgggcata tcggcctccc
     5941 tgcctgacgg gcatttagta acatttttcc aaccgtacga aatgttataa attatcggac
     6001 atcgtaaaac tgttacatta atatgtctat taaatcgtaa atttgtaata atagacatga
     6061 gttgtccgat attcgattta aggtacattt ttatgcgact ttttggttac gctcgggtct
     6121 caaccagtca gcagtctctt gatcttcagg tcagagcact caaagacgca ggtgtgaaag
     6181 caaaccgtat atttaccgat aaggcatccg gcagttcaac agaccgggaa gggctggatt
     6241 tgctgaggat gaaggtggag gaaggtgatg tcattctggt taagaagctc gaccgtcttg
     6301 gccgcgacac tgccgatatg atccaactga taaaggaatt tgacgctcag ggcgtggcag
     6361 tccggttcat tgatgacggg atcagtaccg acggtgatat ggggcaaatg gtggtcacca
     6421 tcctgtcggc tgtggcacag gctgaacgcc ggaggatcct agaacgcacg aatgagggcc
     6481 gacaggaagc aaagctgaaa ggaatcaaat ttggccgcag gcgtaccgtg gacaggaacg
     6541 tcgtgctgac gcttcatcag aagggcactg gtgcaacgga aattgctcat cagctcagta
     6601 ttgcccgctc cacggtttat aaaattcttg aagacgaaag ggcctcgtga tacgcctatt
     6661 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     6721 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6781 catgagacaa taaccctggt aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6841 tcaacatttt cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6901 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6961 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     7021 ttttccaatg atgagcactt ttaaagttct gctatgtggt gcggtattat cccgtgttga
     7081 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     7141 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     7201 tgccataacc atgagtgata acactgctgc caacttactt ctgacaacga tcggaggacc
     7261 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     7321 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
     7381 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     7441 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     7501 tccggctggc tggttttt
//
