LOCUS       NZ_CP014101             8420 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Shigella sonnei strain FDAARGOS_90 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP014101
VERSION     NZ_CP014101.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03996263
            Assembly: GCF_001558295.2
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Campos,J., Goldberg,B., Tallon,L., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J.,
            Nadendla,S., Nandy,P., Hobson,J. and Sichtig,H.
  TITLE     FDA Database for Regulatory Grade Microbial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   Campos,J., Goldberg,B., Tallon,L., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J.,
            Nadendla,S., Nandy,P., Hobson,J. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2017) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 8420)
  AUTHORS   Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Nadendla,S. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2015) Institute for Genome Sciences, University of
            Maryland, 801 W.Baltimore Street, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP3 v. Oct 2014
            Genome Coverage                   :: 255.63x
            Sequencing Technology             :: Pacbio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 04:09:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,070
            CDSs (total)                      :: 4,940
            Genes (coding)                    :: 4,303
            CDSs (with protein)               :: 4,303
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 637
            CDSs (without protein)            :: 637
            Pseudo Genes (ambiguous residues) :: 0 of 637
            Pseudo Genes (frameshifted)       :: 174 of 637
            Pseudo Genes (incomplete)         :: 439 of 637
            Pseudo Genes (internal stop)      :: 110 of 637
            Pseudo Genes (multiple problems)  :: 83 of 637
            Pseudo Genes (short protein)      :: 3 of 637
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP014101.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_90"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_90"
                     /db_xref="taxon:624"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: DC"
                     /collection_date="2013-09-08"
                     /collected_by="Children's National Hospital"
     gene            196..438
                     /locus_tag="AL477_RS25680"
                     /old_locus_tag="AL477_26125"
     CDS             196..438
                     /locus_tag="AL477_RS25680"
                     /old_locus_tag="AL477_26125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(470..1120)
                     /gene="tetR(A)"
                     /locus_tag="AL477_RS25685"
                     /old_locus_tag="AL477_26130"
     CDS             complement(470..1120)
                     /gene="tetR(A)"
                     /locus_tag="AL477_RS25685"
                     /old_locus_tag="AL477_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            1226..2425
                     /gene="tet(A)"
                     /locus_tag="AL477_RS25690"
                     /old_locus_tag="AL477_26135"
     ARG             1227..2422
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<2392..2580)
                     /locus_tag="AL477_RS25695"
                     /old_locus_tag="AL477_29450"
                     /pseudo=""
     CDS             complement(<2392..2580)
                     /locus_tag="AL477_RS25695"
                     /old_locus_tag="AL477_29450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(2624..3013)
                     /locus_tag="AL477_RS25700"
                     /old_locus_tag="AL477_29455"
     CDS             complement(2624..3013)
                     /locus_tag="AL477_RS25700"
                     /old_locus_tag="AL477_29455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            3231..3671
                     /locus_tag="AL477_RS25705"
                     /old_locus_tag="AL477_26140"
     CDS             3231..3671
                     /locus_tag="AL477_RS25705"
                     /old_locus_tag="AL477_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(4460..4609)
                     /locus_tag="AL477_RS26085"
     CDS             complement(4460..4609)
                     /locus_tag="AL477_RS26085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            5180..5338
                     /locus_tag="AL477_RS26730"
                     /old_locus_tag="AL477_29465"
     CDS             5180..5338
                     /locus_tag="AL477_RS26730"
                     /old_locus_tag="AL477_29465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            5798..6613
                     /gene="sul2"
                     /locus_tag="AL477_RS25725"
                     /old_locus_tag="AL477_26145"
     ARG             5799..6610
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6674..7477
                     /gene="aph(3'')-Ib"
                     /locus_tag="AL477_RS25730"
                     /old_locus_tag="AL477_26150"
     ARG             6675..7474
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7477..8310
                     /locus_tag="AL477_RS25735"
                     /old_locus_tag="AL477_26155"
                     /pseudo=""
     CDS             7477..8310
                     /locus_tag="AL477_RS25735"
                     /old_locus_tag="AL477_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /name="APH(6)-I family aminoglycoside O-phosphotransferase"
     ARG             7986..8266
                     /name="APH(6)-Id"
                     /gene="APH(6)-Id"
                     /gene_name="APH(6)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="33.45"
     ARG             7478..8037
                     /name="APH(6)-Id"
                     /gene="APH(6)-Id"
                     /gene_name="APH(6)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.46"
                     /coverage="66.91"
     Typing          complement(4645..4818)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          4742..5347
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 agccccgtag cgacgcggag aggggcgaga caagccaagg gcgcaggctc gatgcgcagc
       61 acgacatagc cggttctcgc aaggacgaga atttccctgc ggtgcccctc aagtgtcaat
      121 gaaagtttcc aacgcgagcc attcgcgaga gccttgagtc cacgctagat ctatctcatc
      181 tgcgcaaggc agaacgtgaa gacggccgcc ctggacctcg cccgcgagcg ccaggcgcac
      241 gaggccggcg cgcggacccg cgccacggcc cacgagcgga cgccgcagca ggagcgccag
      301 aaggccgcca gagaggccga gcgcggccgt gaggcttgga cgctagggca gggcatgaaa
      361 aagcccgtag cgggctgcta cgggcgtctg acgcggtgga aagggggagg ggatgttgtc
      421 tacatggctc tgctgtagtg agtgggttgc gctccggcag cggtcctgat caatcgtcac
      481 cctttctcgg tccttcaacg ttcctgacaa cgagcctcct tttcgccaat ccatcgacaa
      541 tcaccgcgag tccctgctcg aacgctgcgt ccggaccggc ttcgtcgaag gcgtctatcg
      601 cggcccgcaa cagcggcgag agcggagcct gttcaacggt gccgccgcgc tcgccggcat
      661 cgctgtcgcc ggcctgctcc tcaagcacgg ccccaacagt gaagtagctg attgtcatca
      721 gcgcattgac ggcgtccccg gccgaaaaac ccgcctcgca gaggaagcga agctgcgcgt
      781 cggccgtttc catctgcggt gcgcccggtc gcgtgccggc atggatgcgc gcgccatcgc
      841 ggtaggcgag cagcgcctgc ctgaagctgc gggcattccc gatcagaaat gagcgccagt
      901 cgtcgtcggc tctcggcacc gaatgcgtat gattctccgc cagcatggct tcggccagtg
      961 cgtcgagcag cgcccgcttg ttcctgaagt gccagtaaag cgccggctgc tgaaccccca
     1021 accgttccgc cagtttgcgt gtcgtcagac cgtctacgcc gacctcgttc aacaggtcca
     1081 gggcggcacg gatcactgta ttcggctgca actttgtcat gcttgacact ttatcactga
     1141 taaacataat atgtccacca acttatcagt gataaagaat ccgcgcgttc aatcggacca
     1201 gcggaggctg gtccggaggc cagacgtgaa acccaacaga cccctgatcg taattctgag
     1261 cactgtcgcg ctcgacgctg tcggcatcgg cctgattatg ccggtgctgc cgggcctcct
     1321 gcgcgatctg gttcactcga acgacgtcac cgcccactat ggcattctgc tggcgctgta
     1381 tgcgttgatg caatttgcct gcgcacctgt gctgggcgcg ctgtcggatc gtttcgggcg
     1441 gcggccggtc ttgctcgtct cgctggccgg cgctgctgtc gactacgcca tcatggcgac
     1501 ggcgcctttc ctttgggttc tctatatcgg gcggatcgtg gccggcatca ccggggcgac
     1561 tggggcggta gccggcgctt atattgccga tatcactgat ggcgatgagc gcgcgcggca
     1621 cttcggcttc atgagcgcct gtttcgggtt cgggatggtc gcgggacctg tgctcggtgg
     1681 gctgatgggc ggtttctccc cccacgctcc gttcttcgcc gcggcagcct tgaacggcct
     1741 caatttcctg acgggctgtt tccttttgcc ggagtcgcac aaaggcgaac gccggccgtt
     1801 acgccgggag gctctcaacc cgctcgcttc gttccggtgg gcccggggca tgaccgtcgt
     1861 cgccgccctg atggcggtct tcttcatcat gcaacttgtc ggacaggtgc cggccgcgct
     1921 ttgggtcatt ttcggcgagg atcgctttca ctgggacgcg accacgatcg gcatttcgct
     1981 tgccgcattt ggcattctgc attcactcgc ccaggcaatg atcaccggcc ctgtagccgc
     2041 ccggctcggc gaaaggcggg cactcatgct cggaatgatt gccgacggca caggctacat
     2101 cctgcttgcc ttcgcgacac ggggatggat ggcgttcccg atcatggtcc tgcttgcttc
     2161 gggtggcatc ggaatgccgg cgctgcaagc aatgttgtcc aggcaggtgg atgaggaacg
     2221 tcaggggcag ctgcaaggct cactggcggc gctcaccagc ctgacctcga tcgtcggacc
     2281 cctcctcttc acggcgatct atgcggcttc tataacaacg tggaacgggt gggcatggat
     2341 tgcaggcgct gccctctact tgctctgcct gccggcgctg cgtcgcgggc tttggagaaa
     2401 ttcttcaaat tcccgttgca catagcccgg caattccttt ccctgctctg ccataagcgc
     2461 agcgaatgcc gggtaatact cgtcaacgat ctgatagaga agggtttgct cgggtcggtg
     2521 gctctggtaa cgaccagtat cccgatcccg gctggccgtc ctggccgcca catgaggcat
     2581 gttccgcgtc cttgcaatac tgtgtttaca tacagtctat cgcttagcgg aaagttcttt
     2641 taccctcagc cgaaatgcct gccgttgcta gacattgcca gccagtgccc gtcactccca
     2701 ctcttacatt gttcgcagta tctaggtaca tgttattgcg tgcaccttca tgatgtataa
     2761 tgttattaat gacagcagaa agatcgacag aattaatatt cttcgtacct gggacagtta
     2821 tcgggtgtcg gccagtgatg agaccaaagg atgcagtata agatgaacca ctactattat
     2881 catggccacc attaccacca ccgttaaaac tacctcccga acttccagag ccgctatgac
     2941 catcattgta ggggtcattt ggtcccatgt gaccaaaacc accgacagta ccgctattta
     3001 actctgtact cataaaatta tcctctttga cttttaaaaa cagcaaaaac tgtatttata
     3061 acaatgtgtt tatatacagt attcgaaata ctacgcccgt caaaaccaca ccgtcaacag
     3121 gttttttgtg tatttttcac gcctcacggg cagcctcaga ccttttgtgt taaaacagta
     3181 atatgttctg ctgttacaat acggtacaca caggagacac tgtcactttt atggcatggc
     3241 acacagttca agagacactg aaattaacag gaaagtcgcg ctcgcaatta tatcgtgaca
     3301 tggctaaagg ccttgtcagc tatcgcacag gaaaaaatga tcgccgggag ttcgagacca
     3361 gtgaactgat tcgggcctat ggcgagctgc gcacttctga gacaccaaaa aggcacaagg
     3421 agggacactc caaatctgtc gttagccagg cggaaaatgc tcagctagac gtcattcagc
     3481 agaaactgga cgctctgcag cagactgtta ccctaatgct tgaagacaag atggcgcggg
     3541 aagcagaaaa ccgcctgcga aatgaggaga gggaacaact acaggccgaa attagccgtc
     3601 tggctgacgc gctggcacag gaaaaaaagc ggggattctg gtcgcggctg tttcgctccg
     3661 gttcagagta acagtgcagg tgactctgat ttaccataac atctgttatc agcaccgcct
     3721 gagaggccct cagggcgttt ctgacgggaa accggtaaaa caggcaacaa accacccgaa
     3781 aacccgccag aaacgcgcag gctctgtttt tacggcatgc atgagtatgc gcacaagtgc
     3841 atagcgcgga aattcacacc attcagccgg ggtaagtcct gtttccggcg ttcttcccat
     3901 ccgaaaacgg tgaacgggga cgcaatttat tgcgtggccg tgaagcgttg ccggcgcagc
     3961 cggaaaaccc gctgtcagca cgttcaggaa gggggtgatg tacaaatggc acagaggatg
     4021 aaagtgacgt atgagctgaa ggaatgatgt aaaagtgatg tataagtgat gtaaaagtga
     4081 tgtataagtg atgtaaaagt gatgtataag tgatgtaaaa gtgatgtatg aactgaagga
     4141 gcgatgtaat tctgtagtca ggaagaataa cccgttcatg caggcatgaa cgatacagtg
     4201 aggaacgctt tttttgtcac gatctcccct taacctgcgt aaatcacccg ccatggttga
     4261 ttatttttca acctcaaaat ggtcatacac ctaacgcagc cgcgattttc atcgcaactg
     4321 catgaattaa aagaaaaaaa aggaatattt cgttttcacg agtaataccc gggtattaaa
     4381 aatccactgt aattacatga aaataaagaa ataaaaggta ataccggagt attaccacgt
     4441 acgcgggcaa gcgaaaaagc tataacgctt cccggccgac aggcgaccca aaatccacag
     4501 gggcatgcag gttctcttca gtaacctgcg ccattaaatc aaaaaggtca tattcgccgg
     4561 gaagctgcgt atcagtcgaa tccagttgtt catcatattc agatggcatg gggtgatatc
     4621 cagtagctga gaggttgtta gcgggtttcg gggcgcagcc ctgaaccagt catgtagcgc
     4681 tagcggagtg tatactggct tactatgttg ccacgattgc gaaaatcagt gaagtgcttc
     4741 atgtggcagg agaaaaaagg cagcaccggc gcgtcagcag aatatgcgat acaggagata
     4801 ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cggctgcggc gagcggtacc
     4861 ggcttacgga cggggacgga aatttcctgg aagataccag gaagacactt agcaaagaag
     4921 agataaggcc acggcgaagc cgtttttcca taggctccgc ccccctgaca agcatcgcaa
     4981 aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat cccaggcgtt
     5041 tccccctggt agctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat
     5101 tccgctgtta tggccgcggt ttctcattcc acgcctgaca ctcagttccg tgaaggcagt
     5161 tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttgtc
     5221 cggtaactat caacttgagt ccaacccgga aagacacgac aaaacgccac tggcagcagc
     5281 cattagtaac aggagtgcac gagaagatag attaagaggt cttgaagtag aggcctgact
     5341 gcggctacac tgaaagaaca gttttggtaa tggcggtctc gtgaaccctg ttaccgggtt
     5401 aagcagttcc ccaactgact taaccttcga acaaaccgcc tccccaggcg gttttttcgt
     5461 ttacagagca agagattacg attcattgaa aaggatctca agaagatcct ttgtagatta
     5521 ctatcaatta atcttccgaa catgacacca gtcactgttg attacggtta aaccgctccg
     5581 taacaggttt ccctcgtatt ggtgacttgc agcaccaaag aagcaaaaaa gataaatata
     5641 tctacttatt gagagatgat atatttattt ccaaaatgct acagatacat catcatgaaa
     5701 cggatcaccg caaacaggtt actcgcctgg ggattccctt tcgacccgag catccgtatg
     5761 agactcatgc tcgattatta ttattataga agcccccatg aataaatcgc tcatcatttt
     5821 cggcatcgtc aacataacct cggacagttt ctccgatgga ggccggtatc tggcgccaga
     5881 cgcagccatt gcgcaggcgc gtaagctgat ggccgagggg gcagatgtga tcgacctcgg
     5941 tccggcatcc agcaatcccg acgccgcgcc tgtttcgtcc gacacagaaa tcgcgcgtat
     6001 cgcgccggtg ctggacgcgc tcaaggcaga tggcattccc gtctcgctcg acagttatca
     6061 acccgcgacg caagcctatg ccttgtcgcg tggtgtggcc tatctcaatg atattcgcgg
     6121 ttttccagac gctgcgttct atccgcaatt ggcgaaatca tctgccaaac tcgtcgttat
     6181 gcattcggtg caagacgggc aggcagatcg gcgcgaggca cccgctggcg acatcatgga
     6241 tcacattgcg gcgttctttg acgcgcgcat cgcggcgctg acgggtgccg gtatcaaacg
     6301 caaccgcctt gtccttgatc ccggcatggg gttttttctg ggggctgctc ccgaaacctc
     6361 gctctcggtg ctggcgcggt tcgatgaatt gcggctgcgc ttcgatttgc cggtgcttct
     6421 gtctgtttcg cgcaaatcct ttctgcgcgc gctcacaggc cgtggtccgg gggatgtcgg
     6481 ggccgcgaca ctcgctgcag agcttgccgc cgccgcaggt ggagctgact tcatccgcac
     6541 acacgagccg cgccccttgc gcgacgggct ggcggtattg gcggcgctga aagaaaccgc
     6601 aagaattcgt taactgcaca ttcgggatat ttctctatat tcgcgcttca tcagaaaact
     6661 gaaggaacct ccattgaatc gaactaatat tttttttggt gaatcgcatt ctgactggtt
     6721 gcctgtcaga ggcggagaat ctggtgattt tgtttttcga cgtggtgacg ggcatgcctt
     6781 cgcgaaaatc gcacctgctt cccgccgcgg tgagctcgct ggagagcgtg accgcctcat
     6841 ttggctcaaa ggtcgaggtg tggcttgccc cgaggtcatc aactggcagg aggaacagga
     6901 gggtgcatgc ttggtgataa cggcaattcc gggagtaccg gcggctgatc tgtctggagc
     6961 ggatttgctc aaagcgtggc cgtcaatggg gcagcaactt ggcgctgttc acagcctatc
     7021 ggttgatcaa tgtccgtttg agcgcaggct gtcgcgaatg ttcggacgcg ccgttgatgt
     7081 ggtgtcccgc aatgccgtca atcccgactt cttaccggac gaggacaaga gtacgccgca
     7141 gctcgatctt ttggctcgtg tcgaacgaga gctaccggtg cggctcgacc aagagcgcac
     7201 cgatatggtt gtttgccatg gtgatccctg catgccgaac ttcatggtgg accctaaaac
     7261 tcttcaatgc acgggtctga tcgaccttgg gcggctcgga acagcagatc gctatgccga
     7321 tttggcactc atgattgcta acgccgaaga gaactgggca gcgccagatg aagcagagcg
     7381 cgccttcgct gtcctattca atgtattggg gatcgaagcc cccgaccgcg aacgccttgc
     7441 cttctatctg cgattggacc ctctgacttg gggttgatgt tcatgccgcc tgtttttcct
     7501 gctcattggc acgtttcgca acctgttctc attgcggaca ccttttccag cctcgtttgg
     7561 aaagtttcat tgccagacgg gactcctgca atcgtcaagg gattgaaacc tatagaagac
     7621 attgctgatg aactgcgcgg ggccgactat ctggtatggc gcaatgggag gggagcagtc
     7681 cggttgctcg gtcgtgagaa caatctgatg ttgctcgaat atgccgggga gcgaatgctc
     7741 tctcacatcg ttgccgagca cggcgactac caggcgaccg aaattgcagc ggaactaatg
     7801 gcgaagctgt atgccgcatc tgaggaaccc ctgccttctg cccttctccc gatccgggat
     7861 cgctttgcag ctttgtttca gcgggcgcgc gatgatcaaa acgcaggttg tcaaactgac
     7921 tacgtccacg cggcgattat agccgatcaa atgatgagca atgcctcgga actgcgtggg
     7981 ctacatggcg atctgcatca tgaaaacatc atgttctcca gtcgcgctgg ctggtgatag
     8041 atccgtcggt ctggtcggtg aagtgggctt tggcgccgcc aatatgttct acgatccggc
     8101 tgacagagac gacctttgtc tcgatcctag acgcattgca cagatggcgg acgcattctc
     8161 tcgtgcgctg gacgtcgatc gcgtcgcctg ctcgaccagg cgtacgctta tgggtgcctt
     8221 tccgcagctt ggaacgcgga tggagaagag gagcaacgcg atctagctat cgcggccgcg
     8281 atcaagcagg tgcgacagac gtcatactag atatcaagcg acttctccta tcccctggga
     8341 acacatcaat ctcaccggag aatatcgctg gccaaagcct tagcgtagga ttccgcccct
     8401 tcccgcaaac gacccctgga
//
