LOCUS       NZ_CP014100             8421 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Shigella sonnei strain FDAARGOS_90 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP014100
VERSION     NZ_CP014100.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03996263
            Assembly: GCF_001558295.2
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8421)
  AUTHORS   Campos,J., Goldberg,B., Tallon,L., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J.,
            Nadendla,S., Nandy,P., Hobson,J. and Sichtig,H.
  TITLE     FDA Database for Regulatory Grade Microbial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8421)
  AUTHORS   Campos,J., Goldberg,B., Tallon,L., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J.,
            Nadendla,S., Nandy,P., Hobson,J. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2017) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 8421)
  AUTHORS   Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Nadendla,S. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2015) Institute for Genome Sciences, University of
            Maryland, 801 W.Baltimore Street, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP3 v. Oct 2014
            Genome Coverage                   :: 255.63x
            Sequencing Technology             :: Pacbio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 04:09:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,070
            CDSs (total)                      :: 4,940
            Genes (coding)                    :: 4,303
            CDSs (with protein)               :: 4,303
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 637
            CDSs (without protein)            :: 637
            Pseudo Genes (ambiguous residues) :: 0 of 637
            Pseudo Genes (frameshifted)       :: 174 of 637
            Pseudo Genes (incomplete)         :: 439 of 637
            Pseudo Genes (internal stop)      :: 110 of 637
            Pseudo Genes (multiple problems)  :: 83 of 637
            Pseudo Genes (short protein)      :: 3 of 637
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP014100.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_90"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_90"
                     /db_xref="taxon:624"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: DC"
                     /collection_date="2013-09-08"
                     /collected_by="Children's National Hospital"
     gene            complement(join(7999..8421,1..807))
                     /gene="tet(A)"
                     /locus_tag="AL477_RS25615"
                     /old_locus_tag="AL477_26085"
     ARG             complement(2..882)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="69.34"
     gene            913..1563
                     /gene="tetR(A)"
                     /locus_tag="AL477_RS25620"
                     /old_locus_tag="AL477_26090"
     CDS             913..1563
                     /gene="tetR(A)"
                     /locus_tag="AL477_RS25620"
                     /old_locus_tag="AL477_26090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            complement(1595..1837)
                     /locus_tag="AL477_RS25625"
                     /old_locus_tag="AL477_26095"
     CDS             complement(1595..1837)
                     /locus_tag="AL477_RS25625"
                     /old_locus_tag="AL477_26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(2143..2979)
                     /locus_tag="AL477_RS25635"
                     /old_locus_tag="AL477_26105"
     CDS             complement(2143..2979)
                     /locus_tag="AL477_RS25635"
                     /old_locus_tag="AL477_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(2979..3782)
                     /gene="aph(3'')-Ib"
                     /locus_tag="AL477_RS25640"
                     /old_locus_tag="AL477_26110"
     ARG             complement(2983..3782)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(3843..4657)
                     /gene="sul2"
                     /locus_tag="AL477_RS25645"
                     /old_locus_tag="AL477_26115"
                     /pseudo=""
     ARG             complement(3867..4657)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="98.39"
                     /coverage="97.05"
     gene            complement(5117..5275)
                     /locus_tag="AL477_RS26725"
                     /old_locus_tag="AL477_29430"
     CDS             complement(5117..5275)
                     /locus_tag="AL477_RS26725"
                     /old_locus_tag="AL477_29430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            5846..5995
                     /locus_tag="AL477_RS26080"
     CDS             5846..5995
                     /locus_tag="AL477_RS26080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(6784..7224)
                     /locus_tag="AL477_RS25665"
                     /old_locus_tag="AL477_26120"
     CDS             complement(6784..7224)
                     /locus_tag="AL477_RS25665"
                     /old_locus_tag="AL477_26120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            7442..7831
                     /locus_tag="AL477_RS25670"
                     /old_locus_tag="AL477_29440"
     CDS             7442..7831
                     /locus_tag="AL477_RS25670"
                     /old_locus_tag="AL477_29440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            7875..>8057
                     /locus_tag="AL477_RS25675"
                     /old_locus_tag="AL477_29445"
                     /pseudo=""
     CDS             7875..>8057
                     /locus_tag="AL477_RS25675"
                     /old_locus_tag="AL477_29445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     ARG             complement(2147..2979)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          5638..5811
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(5109..5714)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 agggccggtg atcattgcct gggcgagtga atgcagaatg ccaaatgcgg caagcgaaat
       61 gccgatcgtg gtcgcgtccc agtgaaagcg atcctcgccg aaaatgaccc aaagcgcggc
      121 cggcacctgt ccgacaagtt gcatgatgaa gaagaccgcc atcagggcgg cgacgacggt
      181 catgccccgg gcccaccgga acgaagcgag cgggttgaga gcctcccggc gtaacggccg
      241 gcgttcgcct ttgtgcgact ccggcaaaag gaaacagccc gtcaggaaat tgaggccgtt
      301 caaggctgcc gcggcgaaga acggagcgtg gggggagaaa ccgcccatca gcccaccgag
      361 cacaggtccc gcgaccatcc cgaacccgaa acaggcgctc atgaagccga agtgccgcgc
      421 gcgctcatcg ccatcagtga tatcggcaat ataagcgccg gctaccgccc cagtcgcccc
      481 ggtgatgccg gccacgatcc gcccgatata gagaacccaa aggaaaggcg ccgtcgccat
      541 gatggcgtag tcgacagcag cgccggccag cgagacgagc aagaccggcc gccgcccgaa
      601 acgatccgac agcgcgccca gcacaggtgc gcaggcaaat tgcatcaacg catacagcgc
      661 cagcagaatg ccatagtggg cggtgacgtc gttcgagtga accagatcgc gcaggaggcc
      721 cggcagcacc ggcataatca ggccgatgcc gacagcgtcg agcgcgacag tgctcagaat
      781 tacgatcagg ggtctgttgg gtttcacgtc tggcctccgg accagcctcc gctggtccga
      841 ttgaacgcgc ggattcttta tcactgataa gttggtggac atattatgtt tatcagtgat
      901 aaagtgtcaa gcatgacaaa gttgcagccg aatacagtga tccgtgccgc cctggacctg
      961 ttgaacgagg tcggcgtaga cggtctgacg acacgcaaac tggcggaacg gttgggggtt
     1021 cagcagccgg cgctttactg gcacttcagg aacaagcggg cgctgctcga cgcactggcc
     1081 gaagccatgc tggcggagaa tcatacgcat tcggtgccga gagccgacga cgactggcgc
     1141 tcatttctga tcgggaatgc ccgcagcttc aggcaggcgc tgctcgccta ccgcgatggc
     1201 gcgcgcatcc atgccggcac gcgaccgggc gcaccgcaga tggaaacggc cgacgcgcag
     1261 cttcgcttcc tctgcgaggc gggtttttcg gccggggacg ccgtcaatgc gctgatgaca
     1321 atcagctact tcactgttgg ggccgtgctt gaggagcagg ccggcgacag cgatgccggc
     1381 gagcgcggcg gcaccgttga acaggctccg ctctcgccgc tgttgcgggc cgcgatagac
     1441 gccttcgacg aagccggtcc ggacgcagcg ttcgagcagg gactcgcggt gattgtcgat
     1501 ggattggcga aaaggaggct cgttgtcagg aacgttgaag gaccgagaaa gggtgacgat
     1561 tgatcaggac cgctgccgga gcgcaaccca ctcactacag cagagccatg tagacaacat
     1621 cccctccccc tttccaccgc gtcagacgcc cgtagcagcc cgctacgggc tttttcatgc
     1681 cctgccctag cgtccaagcc tcacggccgc gctcggcctc tctggcggcc ttctggcgct
     1741 cctgctgcgg cgtccgctcg tgggccgtgg cgcgggtccg cgcgccggcc tcgtgcgcct
     1801 ggcgctcgcg ggcgaggtcc agggcggccg tcttcacgtt ctgccttgcg cagatgagat
     1861 agatctagcg tggactcaag gctctcgcga atggctcgcg ttggaaactt tcattgacac
     1921 ttgaggggca ccgcagggaa attctcgtcc ttgcgagaac cggctatgtc gtgctgcgca
     1981 tcgagcctgc gcccttggct tgtctcgccc ctctccgcgt cgctacgggg cttccagggg
     2041 tcgtttgcgg gaaggggcgg aatcctacgc taaggctttg gccagcgata ttctccggtg
     2101 agattgatgt gttcccaggg gataggagaa gtcgcttgat atctagtatg acgtctgtcg
     2161 cacctgcttg atcgcggccg cgatagctag atcgcgttgc tcctcttctc catccgcgtt
     2221 ccaagctgcg gaaaggcacc cataagcgta cgcctggtcg agcaggcgac gcggatcgac
     2281 gtccagcgca cgagagaatg cgtccgccat ctgtgcaatg cgtctaggat cgagacaaag
     2341 gtcgtctctg tcagccggat cgtagaacat attggcggcg ccaaagccca cttcaccgac
     2401 cagaccgacg ggatctatca ccagccagcc gcgactggag aacatgatgt tttcatgatg
     2461 cagatcgcca tgtagcccac gcagttccga ggcattgctc atcatttgat cggctataat
     2521 cgccgcgtgg acgtagtcag tttgacaacc tgcgttttga tcatcgcgcg cccgctgaaa
     2581 caaagctgca aagcgatccc ggatcgggag aagggcagaa ggcaggggtt cctcagatgc
     2641 ggcatacagc ttcgccatta gttccgctgc aatttcggtc gcctggtagt cgccgtgctc
     2701 ggcaacgatg tgagagagca ttcgctcccc ggcatattcg agcaacatca gattgttctc
     2761 acgaccgagc aaccggactg ctcccctccc attgcgccat accagatagt cggccccgcg
     2821 cagttcatca gcaatgtctt ctataggttt caatcccttg acgattgcag gagtcccgtc
     2881 tggcaatgaa actttccaaa cgaggctgga aaaggtgtcc gcaatgagaa caggttgcga
     2941 aacgtgccaa tgagcaggaa aaacaggcgg catgaacatc aaccccaagt cagagggtcc
     3001 aatcgcagat agaaggcaag gcgttcgcgg tcgggggctt cgatccccaa tacattgaat
     3061 aggacagcga aggcgcgctc tgcttcatct ggcgctgccc agttctcttc ggcgttagca
     3121 atcatgagtg ccaaatcggc atagcgatct gctgttccga gccgcccaag gtcgatcaga
     3181 cccgtgcatt gaagagtttt agggtccacc atgaagttcg gcatgcaggg atcaccatgg
     3241 caaacaacca tatcggtgcg ctcttggtcg agccgcaccg gtagctctcg ttcgacacga
     3301 gccaaaagat cgagctgcgg cgtactcttg tcctcgtccg gtaagaagtc gggattgacg
     3361 gcattgcggg acaccacatc aacggcgcgt ccgaacattc gcgacagcct gcgctcaaac
     3421 ggacattgat caaccgatag gctgtgaaca gcgccaagtt gctgccccat tgacggccac
     3481 gctttgagca aatccgctcc agacagatca gccgccggta ctcccggaat tgccgttatc
     3541 accaagcatg caccctcctg ttcctcctgc cagttgatga cctcggggca agccacacct
     3601 cgacctttga gccaaatgag gcggtcacgc tctccagcga gctcaccgcg gcgggaagca
     3661 ggtgcgattt tcgcgaaggc atgcccgtca ccacgtcgaa aaacaaaatc accagattct
     3721 ccgcctctga caggcaacca gtcagaatgc gattcaccaa aaaaaatatt agttcgattc
     3781 aatggaggtt ccttcagttt tctgatgaag cgcgaatata gagaaatatc ccgaatgtgc
     3841 agttaacgaa ttcttgcggt ttctttcagc gccgccaata ccgccagccc gtcgcgcaag
     3901 gggcgcggct cgtgtgtgcg gatgaagtca gctccacctg cggcggcggc aagctctgca
     3961 gcgagtgtcg cggccccgac atcccccgga ccacggcctg tgagcgcgcg cagaaaggat
     4021 ttgcgcgaaa cagacagaag caccggcaaa tcgaagcgca gccgcaattc atcgaaccgc
     4081 gccagcaccg agagcgaggt ttcgggagca gccccagaaa aaaccccatg ccgggatcaa
     4141 ggacaaggcg gttgcgtttg ataccggcac ccgtcagcgc cgcgatgcgc gcgtcaaaga
     4201 acgccgcaat gtgatccatg atgtcgccag cgggtgcctc gcgccgatct gcctgcccgt
     4261 cttgcaccga atgcataacg acgagtttgg cagatgattt cgccaattgc ggatagaacg
     4321 cagcgtctgg aaaaccgcga atatcattga gataggccac accacgcgac aaggcatagg
     4381 cttgcgtcgc gggttgataa ctgtcgagcg agacgggaat gccatctgcc ttgagcgcgt
     4441 ccagcaccgg cgcgatacgc gcgatttctg tgtcggacga aacaggcgcg gcgtcgggat
     4501 tgctggatgc cggaccgagg tcgatcacat ctgccccctc ggccatcagc ttacgcgcct
     4561 gcgcaatggc tgcgtctggc gccagatacc ggcctccatc ggagaaactg tccgaggtta
     4621 tgttgacgat gccgaaaatg atgagcgatt tattcatggg ggcttctata ataataataa
     4681 tcgagcatga gtctcatacg gatgctcggg tcgaaaggga atccccaggc gagtaacctg
     4741 tttgcggtga tccgtttcat gatgatgtat ctgtagcatt ttggaaataa atatatcatc
     4801 tctcaataag tagatatatt tatctttttt gcttctttgg tgctgcaagt caccaatacg
     4861 agggaaacct gttacggagc ggtttaaccg taatcaacag tgactggtgt catgttcgga
     4921 agattaattg atagtaatct acaaaggatc ttcttgagat ccttttcaat gaatcgtaat
     4981 ctcttgctct gtaaacgaaa aaaccgcctg gggaggcggt ttgttcgaag gttaagtcag
     5041 ttggggaact gcttaacccg gtaacagggt tcacgagacc gccattacca aaactgttct
     5101 ttcagtgtag ccgcagtcag gcctctactt caagacctct taatctatct tctcgtgcac
     5161 tcctgttact aatggctgct gccagtggcg ttttgtcgtg tctttccggg ttggactcaa
     5221 gttgatagtt accggacaag gcgcagcggt cggactgaac ggggggttcg tgcatacagt
     5281 ccagcttgga gcgaactgcc ttcacggaac tgagtgtcag gcgtggaatg agaaaccgcg
     5341 gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg
     5401 agctaccagg gggaaacgcc tgggatcttt atagtcctgt cgggtttcgc caccactgat
     5461 ttgagcgtca gattttgcga tgcttgtcag gggggcggag cctatggaaa aacggcttcg
     5521 ccgtggcctt atctcttctt tgctaagtgt cttcctggta tcttccagga aatttccgtc
     5581 cccgtccgta agccggtacc gctcgccgca gccgaacgac cgagcgtagc gagtcagtga
     5641 gcgaggaagc ggaatatctc ctgtatcgca tattctgctg acgcgccggt gctgcctttt
     5701 ttctcctgcc acatgaagca cttcactgat tttcgcaatc gtggcaacat agtaagccag
     5761 tatacactcc gctagcgcta catgactggt tcagggctgc gccccgaaac ccgctaacaa
     5821 cctctcagct actggatatc accccatgcc atctgaatat gatgaacaac tggattcgac
     5881 tgatacgcag cttcccggcg aatatgacct ttttgattta atggcgcagg ttactgaaga
     5941 gaacctgcat gcccctgtgg attttgggtc gcctgtcggc cgggaagcgt tatagctttt
     6001 tcgcttgccc gcgtacgtgg taatactccg gtattacctt ttatttcttt attttcatgt
     6061 aattacagtg gatttttaat acccgggtat tactcgtgaa aacgaaatat tccttttttt
     6121 tcttttaatt catgcagttg cgatgaaaat cgcggctgcg ttaggtgtat gaccattttg
     6181 aggttgaaaa ataatcaacc atggcgggtg atttacgcag gttaagggga gatcgtgaca
     6241 aaaaaagcgt tcctcactgt atcgttcatg cctgcatgaa cgggttattc ttcctgacta
     6301 cagaattaca tcgctccttc agttcataca tcacttttac atcacttata catcactttt
     6361 acatcactta tacatcactt ttacatcact tatacatcac ttttacatca ttccttcagc
     6421 tcatacgtca ctttcatcct ctgtgccatt tgtacatcac ccccttcctg aacgtgctga
     6481 cagcgggttt tccggctgcg ccggcaacgc ttcacggcca cgcaataaat tgcgtccccg
     6541 ttcaccgttt tcggatggga agaacgccgg aaacaggact taccccggct gaatggtgtg
     6601 aatttccgcg ctatgcactt gtgcgcatac tcatgcatgc cgtaaaaaca gagcctgcgc
     6661 gtttctggcg ggttttcggg tggtttgttg cctgttttac cggtttcccg tcagaaacgc
     6721 cctgagggcc tctcaggcgg tgctgataac agatgttatg gtaaatcaga gtcacctgca
     6781 ctgttactct gaaccggagc gaaacagccg cgaccagaat ccccgctttt tttcctgtgc
     6841 cagcgcgtca gccagacggc taatttcggc ctgtagttgt tccctctcct catttcgcag
     6901 gcggttttct gcttcccgcg ccatcttgtc ttcaagcatt agggtaacag tctgctgcag
     6961 agcgtccagt ttctgctgaa tgacgtctag ctgagcattt tccgcctggc taacgacaga
     7021 tttggagtgt ccctccttgt gcctttttgg tgtctcagaa gtgcgcagct cgccataggc
     7081 ccgaatcagt tcactggtct cgaactcccg gcgatcattt tttcctgtgc gatagctgac
     7141 aaggccttta gccatgtcac gatataattg cgagcgcgac tttcctgtta atttcagtgt
     7201 ctcttgaact gtgtgccatg ccataaaagt gacagtgtct cctgtgtgta ccgtattgta
     7261 acagcagaac atattactgt tttaacacaa aaggtctgag gctgcccgtg aggcgtgaaa
     7321 aatacacaaa aaacctgttg acggtgtggt tttgacgggc gtagtatttc gaatactgta
     7381 tataaacaca ttgttataaa tacagttttt gctgttttta aaagtcaaag aggataattt
     7441 tatgagtaca gagttaaata gcggtactgt cggtggtttt ggtcacatgg gaccaaatga
     7501 cccctacaat gatggtcata gcggctctgg aagttcggga ggtagtttta acggtggtgg
     7561 taatggtggc catgataata gtagtggttc atcttatact gcatcctttg gtctcatcac
     7621 tggccgacac ccgataactg tcccaggtac gaagaatatt aattctgtcg atctttctgc
     7681 tgtcattaat aacattatac atcatgaagg tgcacgcaat aacatgtacc tagatactgc
     7741 gaacaatgta agagtgggag tgacgggcac tggctggcaa tgtctagcaa cggcaggcat
     7801 ttcggctgag ggtaaaagaa ctttccgcta agcgatagac tgtatgtaaa cacagtattg
     7861 caaggacgcg gaacatgcct catgtggcgg ccaggacggc cagccgggat cgggatactg
     7921 gtcgttacca gagccaccga cccgagcaaa cccttctcta tcagatcgtt gacgagtatt
     7981 acccggcatt cgctgcgctt atggcagagc agggaaagga attgccgggc tatgtgcaac
     8041 gggaatttga agaattctcc aaagcccgcg acgcagcgcc ggcaggcaga gcaagtagag
     8101 ggcagcgcct gcaatccatg cccacccgtt ccacgttgtt atagaagccg catagatcgc
     8161 cgtgaagagg aggggtccga cgatcgaggt caggctggtg agcgccgcca gtgagccttg
     8221 cagctgcccc tgacgttcct catccacctg cctggacaac attgcttgca gcgccggcat
     8281 tccgatgcca cccgaagcaa gcaggaccat gatcgggaac gccatccatc cccgtgtcgc
     8341 gaaggcaagc aggatgtagc ctgtgccgtc ggcaatcatt ccgagcatga gtgcccgcct
     8401 ttcgccgagc cgggcggcta c
//
