LOCUS       NZ_CP014098             8141 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Shigella sonnei strain FDAARGOS_90 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP014098
VERSION     NZ_CP014098.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03996263
            Assembly: GCF_001558295.2
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   Campos,J., Goldberg,B., Tallon,L., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J.,
            Nadendla,S., Nandy,P., Hobson,J. and Sichtig,H.
  TITLE     FDA Database for Regulatory Grade Microbial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8141)
  AUTHORS   Campos,J., Goldberg,B., Tallon,L., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J.,
            Nadendla,S., Nandy,P., Hobson,J. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2017) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 8141)
  AUTHORS   Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N.,
            Ott,S., Godinez,A., Nagaraj,S., Nadendla,S. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2015) Institute for Genome Sciences, University of
            Maryland, 801 W.Baltimore Street, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP3 v. Oct 2014
            Genome Coverage                   :: 255.63x
            Sequencing Technology             :: Pacbio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 04:09:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,070
            CDSs (total)                      :: 4,940
            Genes (coding)                    :: 4,303
            CDSs (with protein)               :: 4,303
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 637
            CDSs (without protein)            :: 637
            Pseudo Genes (ambiguous residues) :: 0 of 637
            Pseudo Genes (frameshifted)       :: 174 of 637
            Pseudo Genes (incomplete)         :: 439 of 637
            Pseudo Genes (internal stop)      :: 110 of 637
            Pseudo Genes (multiple problems)  :: 83 of 637
            Pseudo Genes (short protein)      :: 3 of 637
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP014098.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8141
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_90"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_90"
                     /db_xref="taxon:624"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: DC"
                     /collection_date="2013-09-08"
                     /collected_by="Children's National Hospital"
     gene            join(6557..8141,1..638)
                     /gene="mobQ"
                     /locus_tag="AL477_RS00595"
                     /old_locus_tag="AL477_00490"
                     /pseudo=""
     CDS             join(6557..8141,1..638)
                     /gene="mobQ"
                     /locus_tag="AL477_RS00595"
                     /old_locus_tag="AL477_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /name="mobQ"
     gene            871..2616
                     /gene="col"
                     /locus_tag="AL477_RS00600"
                     /old_locus_tag="AL477_00495"
     CDS             871..2616
                     /gene="col"
                     /locus_tag="AL477_RS00600"
                     /old_locus_tag="AL477_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="WP_000012960.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFDRFAHDPMSGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKA
                     TGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRRKFWEEVSKDPELSKQ
                     FNPGNKKRLSQGLAPRARNKDTVGGRRSFELHHDKPISQDGGVYDMDNLRITTPKRHID
                     IHRGQ"
                     /name="col"
     gene            2619..2876
                     /locus_tag="AL477_RS00605"
                     /old_locus_tag="AL477_00500"
     CDS             2619..2876
                     /locus_tag="AL477_RS00605"
                     /old_locus_tag="AL477_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000421100.1"
                     /translation="MELKNSISDYTETEFKKIIEDIINCEGDEKKQDDNLEHFISVTEH
                     PSGSDLIYYPEGNNDGSPEAVIKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            2938..3081
                     /locus_tag="AL477_RS00610"
                     /old_locus_tag="AL477_00505"
     CDS             2938..3081
                     /locus_tag="AL477_RS00610"
                     /old_locus_tag="AL477_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001314446.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            3545..3685
                     /locus_tag="AL477_RS00625"
                     /old_locus_tag="AL477_26305"
     CDS             3545..3685
                     /locus_tag="AL477_RS00625"
                     /old_locus_tag="AL477_26305"
                     /inference="COORDINATES: protein motif:HMM:NF020569.4"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]; GO:0043022 - ribosome binding
                     [Evidence IEA]; GO:0003723 - RNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /protein_id="WP_231237649.1"
                     /translation="MYEWDSQHGELEGYRASDGQHLGSFDPKTGNQLKGPDPKRNIKKY
                     L"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            3695..3952
                     /locus_tag="AL477_RS00630"
                     /old_locus_tag="AL477_00515"
     CDS             3695..3952
                     /locus_tag="AL477_RS00630"
                     /old_locus_tag="AL477_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_000523347.1"
                     /translation="MGLKLHINWFDKTTEEFKGGEYSKDFGDDGSVIERLGMPLKDNIN
                     NGWFDVIAEWVPLLQPYFNHQIDISGNEYFVSFDYRDGDW"
                     /name="colicin E3-like toxin immunity protein"
     gene            3972..4238
                     /locus_tag="AL477_RS00635"
                     /old_locus_tag="AL477_00520"
     CDS             3972..4238
                     /locus_tag="AL477_RS00635"
                     /old_locus_tag="AL477_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000861936.1"
                     /translation="MKVELKKSISDYTEAEFLELVKKICRAEGATEEDDNKLVREFERL
                     TEHPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            4300..4443
                     /locus_tag="AL477_RS00640"
                     /old_locus_tag="AL477_00525"
     CDS             4300..4443
                     /locus_tag="AL477_RS00640"
                     /old_locus_tag="AL477_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000724382.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGWTVSPSSTAELTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            4972..5886
                     /locus_tag="AL477_RS00650"
                     /old_locus_tag="AL477_00530"
     CDS             4972..5886
                     /locus_tag="AL477_RS00650"
                     /old_locus_tag="AL477_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001277132.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQP
                     HALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASASA
                     LRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLSASA
                     RRSVDKNYGLGRNCYLFEKGRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQATRGRKGGSKSKRGTVATSARTL
                     KPWEKLGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(6103..6378)
                     /locus_tag="AL477_RS00655"
                     /old_locus_tag="AL477_00535"
     CDS             complement(6103..6378)
                     /locus_tag="AL477_RS00655"
                     /old_locus_tag="AL477_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061066728.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPRPPAKPQNH"
                     /name="hypothetical protein"
     BGC             1..7616
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(6383..6556)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(6557..6915)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.444"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4973..5886
                     /name="rep_cluster_2134"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4..635
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.53e-137"
     Typing          6558..8140
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.295"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctgagcgtgt gagaatgtat tccggtgcga cggtccggca tgtactggaa cagatgcgcc
       61 aggggtggcc ctcttacggt tttccggcgc tgccgcatca ctggccggat aatttttatt
      121 tcagcgacga ccgcaggccc gtagcctctc cgctgccgtc agcgcaccgg gtggatatca
      181 ccgctgatgc tgtaccggaa caactcatgc ctgttgtatt ttcgagagag ggaaacagca
      241 ggacgctgaa tctgttgtta tgcaaagggc cggaggaaat gcttgtcggg tttgttcgcc
      301 gggaaggagg gttttgcccc gttcttgtgc ttccgtcgcc ggattacagt catctgatag
      361 tcagcaccat cacggagaac ggggtatgcc tggcaggtta cggagaagct ataaaccatg
      421 atgaggatac tccgtaccca ccggaaccgc acctgatgca gttccggctc aaaggccatc
      481 atgacaggct tttggctgct gtccacaaac cggaagagat gccggattat ctcttccgtc
      541 aactcggttt taatcagacc tggcatgagt ggaagcggga cgaacagcac aggcaacaac
      601 aacgccgccc cgggcacttc cggggcatga gtatgtgatg tccggggctg caccccggac
      661 cccgccaaca catcacgggc cacaaaattt tttgtggccc gctctgcgtt ttctaagtgt
      721 tatccctcct gatttctaaa aaattttcca cctgagcttg acagaaaaaa cgatgacggg
      781 tactttttga tctgtacata aaaccagtgg ttttatgtac agtattaatc gtgtaattaa
      841 ttgttttaac gcttaaaaga gggaattttt atgagcggtg gcgatggacg cggccataac
      901 acgggcgcgc atagcacaag tggtaacatt aatggtggcc cgaccgggct tggtgtaggt
      961 ggtggtgctt ctgatggttc cgggtggagt tcggaaaata acccgtgggg tggtggttcc
     1021 ggtagcggca ttcactgggg aggtggttcc ggtcatggta atggcggggg gaatggtaat
     1081 tccggtggtg gctcgggaac aggcggtaat ctgtcagcag tagctgcgcc agtggcattt
     1141 ggttttccgg cactttccac tccaggagct ggcggtctgg cggtcagtat ttcagcggga
     1201 gcattatcgg cagctattgc tgatattatg gctgccctga aaggaccgtt taaatttggt
     1261 ctttgggggg tggctttata tggtgtattg ccatcacaaa tagcgaaaga tgaccccaat
     1321 atgatgtcaa agattgtgac gtcattaccc gcagatgata ttactgaatc acctgtcagt
     1381 tcattacctc tcgataaggc aacagtaaac gtaaatgttc gtgttgttga tgatgtaaaa
     1441 gacgaacgac agaatatttc ggttgtttca ggtgttccga tgagtgttcc ggtggttgat
     1501 gcaaaaccta ccgaacgtcc gggtgttttt acggcatcaa ttccaggtgc acctgttctg
     1561 aatatttcag ttaataacag tacgccagca gtacagacat taagcccagg tgttacaaat
     1621 aatactgata aggatgttcg cccggcagga tttactcagg gtggtaatac cagggatgca
     1681 gttattcgat tcccgaagga cagcggtcat aatgctgtat atgtttcagt gagtgatgtt
     1741 cttagccctg accaggtaaa acaacgtcag gatgaagaaa atcgccgtca gcaggaatgg
     1801 gatgctacgc atccggttga agcggctgag cgaaattatg aacgcgcgcg tgcagagctg
     1861 aatcaggcaa atgaagatgt tgccagaaat caggagcgac aggctaaagc tgttcaggtt
     1921 tataattcgc gtaaaagcga acttgatgca gcgaataaga ctcttgctga tgcaatagct
     1981 gaaataaaac aatttgatcg atttgcccat gacccaatgt ctggcggtca cagaatgtgg
     2041 caaatggccg ggcttaaagc ccagcgggcg cagacggatg taaataataa gcaggctgca
     2101 tttgatgctg ctgcaaaaga gaagtcagat gctgatgctg cattaagtgc cgcgcaggag
     2161 cgccgcaaac agaaggaaaa taaagaaaag gacgctaagg ataaattaga taaggagagt
     2221 aaacggaata agccagggaa ggcgacaggt aaaggtaaac cagttggtga taaatggctg
     2281 gatgatgcag gtaaagattc aggagcgcca attccagatc gcattgctga taagttgcgt
     2341 gataaagaat ttaaaaactt cgacgatttt cggagaaaat tctgggaaga agtgtcgaaa
     2401 gatcctgagt taagtaagca atttaatcca ggtaataaaa aacgcttgag ccaaggatta
     2461 gctccacggg ctaggaataa agatactgtc ggtgggcgtc gctcatttga gcttcatcac
     2521 gacaagccaa tcagtcagga tggtggtgtt tatgatatgg acaacctccg tatcactacg
     2581 ccgaaacgcc atattgatat tcacagaggt cagtaaaaat ggaactgaaa aacagcatta
     2641 gtgattacac tgaaactgaa ttcaaaaaaa ttattgaaga catcatcaat tgtgaaggtg
     2701 atgaaaaaaa acaggatgat aacctcgagc attttataag tgttactgag catcctagtg
     2761 gttctgatct gatttattac ccagaaggta ataatgatgg tagccctgaa gctgttatta
     2821 aagagattaa agaatggcga gctgctaacg gtaagtcagg atttaaacag ggctgaaata
     2881 tgaatgccag ttgtttaagg atgaatgact ggcattcttt cacaacaagg agtcgttatg
     2941 aaaaaaataa cagggattat tttattgctt cttgcagtca ttattctggc tgcatgtcag
     3001 gcaaactata tccgggatgt tcagggcggg accgtatctc cgtcatcaac agctgaagtg
     3061 accggattag caacgcagta acccgaaatc ctctttgaca aaaacaaagc gtgtcaggct
     3121 gattctgatg cgcttttttt gaaatgtcac aaaagttcca tgtgggagat gggatctaaa
     3181 atccctgtgc agaaccttct atccggtggg agaaaacttg tcattttgac ctgttcgcca
     3241 ttcggggggg cgcttggaaa acggaaattt tcccacgcta agagaattga gttgaacgag
     3301 tggttacatc taggccagct aactcactga acgtaaaggc tgtacgaaat gttccgaccg
     3361 tccgataacc atttggctta cttccgtaca tttccttgcg tagatgtaca gaattgtacg
     3421 atttcggaca ccaaaaccgc tgttgtcccg ccattctggc gattcctgaa agttatttga
     3481 ctaaaaaata gtcttaacgt ggtttatttt ccgaattcca agcggacccc ataaaggccg
     3541 taagatttat gaatgggact cccagcacgg tgaacttgaa gggtatcgtg ccagtgatgg
     3601 tcagcatctt ggctcattcg atcctaagac tggtaatcag ttgaaaggac ctgatccaaa
     3661 acgaaatatc aaaaagtatc tttgagagga tgttatgggg cttaaattac atattaattg
     3721 gtttgataag acgaccgagg aatttaaagg tggtgagtat tcaaaagatt tcggtgatga
     3781 tggctcggtc attgaacgtc ttggaatgcc tttaaaagat aatatcaata atggttggtt
     3841 tgatgttata gctgaatggg tacctttgct acaaccatac tttaatcatc aaattgatat
     3901 ttccggtaat gagtattttg tttcatttga ttatcgtgat ggtgattggt gatcaaatat
     3961 tctcaggaga gatgaaggtg gagctaaaga aaagtattag tgattatacc gaggctgaat
     4021 ttctggagct tgtaaaaaaa atatgtagag ctgaaggtgc tactgaagag gatgacaata
     4081 aattagtgag agagtttgag cgattaactg agcacccaga tggttcagat ctgatttatt
     4141 atcctcgcga tgacagggaa gatagtcctg aagggattgt caaggaaatt aaagaatggc
     4201 gagctgctaa cggtaagtca ggatttaaac agggctgaaa tatgaatgcc ggttgtttaa
     4261 ggatgaatga ctggcattct ttcacaacaa ggagtcgtta tgaaaaaaat aacagggatt
     4321 attttattgc ttcttgcagt cattattctg gctgcatgtc aggcaaacta tatccgggat
     4381 gttcagggct ggactgtatc cccgtcatca acagctgaac tgaccggatt agcaacgcag
     4441 taacccgaaa tcctctttga caaaaacaaa gcgtgtcagg ctgattctga tgcgcttttt
     4501 tttgaaatgt cacaaaaatt ccatgtggga gatgggatct aaaattctcg tgcagaactt
     4561 tccatccagg gggagaaaac ttgtcgtttt gagccgttcg gtgttcagaa cgcacgaaac
     4621 cgatcgtgta tcgctttcgt gcatagttat gcaacgcgct aaaaacgatt ctgacgcatt
     4681 ttttatgttt accctggtgt ttccttgtct ttttgtgttt tttgcgtcag aaagcgattg
     4741 agggtgtttt aaggggtacg tacaacggga gttatggtaa atggatcggt ttttcgggaa
     4801 ggatcgacag gatttgccgt tgggtgtagt gtaagcgact gaaaaacaaa cgccccgtaa
     4861 atcgtgctct caccgccaag attgatcacg aaattacagg gcgccgggtt ccgcgtttcc
     4921 cgatgggaaa gcgcggttag ttaaactgtg taccgagaga aatcgtatca catgagcgcc
     4981 gtacttcaac gcttcaggga aaaattaccg cacaaaccgt actgtacgaa cgatttcgcg
     5041 tacggcgttc gcattctgcc gaaaaacatt gccattcttg cccgtttcat ccagcagaac
     5101 cagccacatg cactgtactg gcttcccttt gacgtggacc ggacgggggc atcaatcgac
     5161 tggagcgacc ggaattgtcc ggccccgaac atcaccgtaa aaaatccccg taacgggcac
     5221 gcgcatctgc tctacgcgct cgcccttcct gtgagaactg cgccggatgc atcggcttcg
     5281 gcgctcagat acgctgccgc tattgagcgt gcgttgtgtg aaaaactggg cgcggatgtg
     5341 aattacagcg gcctgatctg caaaaatccg tgccaccctg aatggcagga agtggaatgg
     5401 cgcgaggaac cctacactct cgacgaactg gctgattatc tcgatttgag cgcctcagcg
     5461 cgccgtagcg tcgataaaaa ttacgggctg gggcgaaact gctatctgtt cgaaaagggc
     5521 cgtaaatggg cttaccgggc tattcgtcag ggctggccgg cattctcaca atggcttgat
     5581 gcggtgattc agcgtgtcga aatgtacaac gcatcgctcc ccgttccgct ttcacctcct
     5641 gaatgtcggg ctattggcaa gagtattgcg aaatacacgc acaggaactt cacgccggaa
     5701 actttcgcac agtatgtggc tgatacgcac acgccagaaa ttcaggctac acgcggtcgc
     5761 aagggcggtt ctaagtctaa gcgcggcaca gtagctacat cagcacgcac gctgaaacct
     5821 tgggagaaat tagggatcag tcgcgcctgg tattaccaac tgaaaaaacg aggtctcgta
     5881 gagtagacca aataagccta tatcagataa cagcgccttt ttggcgcctt tttgagcagc
     5941 ttggtttgtt gctatttccc tcgttgaatc ccgcaatggt gtggctttcc gcatgattga
     6001 ggtggtagcg ctcgccgcag tctcatgtcc gagcgtagcg agcgaatgag cgaggaagcg
     6061 caaaggcgtc cggtggtgca tgtggcactt acgcgccggg gcttagtggt tctgcggttt
     6121 cgccggtggt ctgggtagct tctccagctc gttaatcagc ggttgtagtc ggttcacatc
     6181 cgcctgtctt gtgacttcct ttcgcagaaa ctggagcagg aacgcacgca gttgcgcttc
     6241 ttccggcctc cgtacccttg ccagcatggc tgcccccaca atgacttttt gcgccgtgtc
     6301 caggcttcgg ctcttcgcct tcaggcgctg taatctggcc tcagcgtcgg caatcttctg
     6361 ttcgagtgtt ctgctcattt cgtgactccg tgcgcggtga aaaatcgcat tttagcgcgt
     6421 cactggtagt tgcaaaactg aactggcata atgcacggca catcacgaag tgcgcactta
     6481 tacaatctcc acttcatttc gattgtgtgc ggtctgcgac gctaaaagaa aacggcaaaa
     6541 aggcattacg gcagaaatgg cgatttatca tctcagcatg aaaatcattt cgcgaaaaaa
     6601 cggctacagt gccgttgctt ctgctgccta ccgttccggc tctgtcatac ccgatgaccg
     6661 taccggatta acccacgatt acacccgtaa acgcggcgtt gatgatgcgg tcattctcac
     6721 ccctgcgaat gcaccgtcct ggtgtggtga ccgttccgtt ctctggaatg cggtcgagaa
     6781 agccgaacag cgccggaact cccagctggc aagggagatt gaactcgcca ttccccgtga
     6841 gatttcccgc gaggccgcac gggagaccgt tctcgctttt gtccgggaaa actttgtcag
     6901 tcggggcatg attgccgatg tggcgttcca tcacatggac cggaccaatc cccatgcgca
     6961 catcatgctg accacgagag ctgtcgggga aacgggattt gcaggaaagg tcagggactg
     7021 gaacgaccgg gcactcgccg agacgtggcg cgcatcatgg gctgaccatg cgaacagagc
     7081 gcttgcgaac gccggctacc aggaagagat agaccatcgt tcatacgagc gtcagggact
     7141 ggagaaagcg cccggccttc acctcggaaa ggctgcctgt gcgatggaaa aacggggaat
     7201 ggaaacagaa cgcggtgagc agaaccgtct gattaacagc cttaacctgg aaatacaggt
     7261 ttcccgcaca aggctggcag tcatccaggt acaggagaac gtgcggaaac gggaactcag
     7321 cgatgccgca cgtcgtgcag cggaagccct taacctgacc attcccgctg cgaatgcctc
     7381 agcggatacc ctgcgggaat tcattgccac gttgccgcat tcctgcggca acgcgtggga
     7441 gatgaccccg gaattcctgg cgatgagcgg gaaggtgaac gacatcgaac gtgaggggaa
     7501 tgcgctgctg aaagagcagg ccattctcga aaaggagatg accggactca aaaaagcacg
     7561 ccctgtcgcg tcccttctgt cagagatccc cctgatgaca tgggctgaac cggaataccg
     7621 caaaagacaa ctccgtttct ggaaactcgg gaaacagatt gaatctcttc gccgcaccta
     7681 cagggccgtg aaagaacggg acattcccgc ccgtcgtcag gcctttgaaa cgcagtggaa
     7741 tacgtggatt gcgccgggaa tggcagagct gaaagaaaaa ctgtcagcac gggaagcgga
     7801 gcggcgcagg gaggaagccg aagcggaagc gcgccggaag gaacaggagc atgaggcgcg
     7861 gctgaaacgt catgataacc accgtctgag ccgtgaaacg gcattagtcg gggttattac
     7921 ggagctgggg cgtgccagag agccgggaac gggcaggata acccgctaca tgatgctgag
     7981 taacagagcc ggagaattca cggtatgggg tgatgagctg gcgcattacc cccagagtgt
     8041 tcatgacccg gtgaatgttt acctgtcgcc aggcggggct gtgatggtct cggatatacg
     8101 tgagggaatg ccagaatctc atgagacgat ggcgcggcct g
//
