LOCUS       NZ_CP013626             2992 bp    DNA     circular CON 06-MAR-2024
DEFINITION  Staphylococcus aureus strain RIVM4296 plasmid pRIVM4296, complete
            sequence.
ACCESSION   NZ_CP013626
VERSION     NZ_CP013626.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04325948
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Bosch,T., Witteveen,S., Haenen,A., Landman,F. and Schouls,L.M.
  TITLE     Next-Generation Sequencing Confirms Presumed Nosocomial Transmission
            of Livestock-Associated Methicillin-Resistant Staphylococcus aureus
            in the Netherlands
  JOURNAL   Appl Environ Microbiol 82 (14), 4081-4089 (2016)
   PUBMED   27129960
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Witteveen,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2015) Laboratory for Infectious Diseases &
            Screening, National Institute for Public Health & the Environment
            (RIVM), Antonie van Leeuwenhoeklaan 9, Bilthoven 3721 MA, The
            Netherlands
COMMENT     ##Assembly-Data-START##
            Assembly Method :: CLC Genomics Workbench v. version 8.5.1
            Sequencing Technology        :: 454; Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 03/06/2024 01:45:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 2
            CDSs (total)                 :: 2
            Genes (coding)               :: 2
            CDSs (with protein)          :: 2
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP013626.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="RIVM4296"
                     /isolation_source="unavailable"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pRIVM4296"
                     /geo_loc_name="Netherlands: Bilthoven"
                     /lat_lon="52.13 N 5.210 E"
                     /collection_date="2009-10-09"
                     /collected_by="Dutch National Institute for Public Health
                     and the Environment (RIVM)"
     gene            complement(848..1852)
                     /locus_tag="AUC54_RS00005"
                     /old_locus_tag="AUC54_00005"
     CDS             complement(848..1852)
                     /locus_tag="AUC54_RS00005"
                     /old_locus_tag="AUC54_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020368559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_078099048.1"
                     /translation="MQDNHTKYIDENQDNKTLKDLTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNVLEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQRVI
                     EEVVKEKPKARWLFLTLSTRNAIDGDTLEKSLKHLTESFRRLFKYKKVSKNLIGFMRST
                     EVTVNKNDGSYNQHMHVLLCVESKYFRGSENYISQNDWINLWQKALQVNYRPVANIKAI
                     KPNQKGDKDIQAAIKETSKYSVKSSDFLTDDDERNQEIVNDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDAEDGNLINTSDEDKTTDEEEKAHSITAIWNFEKQNYYLKDLKR"
                     /name="protein rep"
     gene            complement(2036..2857)
                     /locus_tag="AUC54_RS00010"
                     /old_locus_tag="AUC54_00010"
     CDS             complement(2036..2857)
                     /locus_tag="AUC54_RS00010"
                     /old_locus_tag="AUC54_00010"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RNA methyltransferase"
                     /protein_id="WP_049948317.1"
                     /translation="MFFKCSICDEKLKRGYKTLACENNHTFDIAKEGYSNLISGHKKSK
                     YDKKLFIARRELNDSSNLYSILISKIIEIIKKYEIKTKNNVIDVGTGEGYILNEISKTF
                     LGENEFLGIDIAKEGVKKAAKSYKNINWLVADLADIPVMNGNVDFLINVLSPSNYDEFK
                     RVLDKEGKLIKIIPNSDYLKEIRNIVFSNHKNKDYDNSEVIDLFEANFNKIEMIDISYK
                     VKLNKEELNKLLDMTPLTWNLSDVEKEGIIKKNLSEITMSFKVLVGGFKTS"
                     /name="putative RNA methyltransferase"
     Typing          complement(849..1852)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.949"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cattcgtctg caagtttagt taagggttct tctcaacatc aataaatttt ctcggcataa
       61 atgcgtgttc ttttcttgtc tgcccagtag acaaagtaga cccaactgtt attgctttac
      121 aggtctattt ttcgtcgtta taatctagtt aaatcccgta agggcgcact tatgcgttct
      181 aaaaagaacg cgtaaaaaac cataatcgac cttcgtcaat ttttatctta gttttaagga
      241 atcggcgttg tggcgcagat ataaaaatta atactgcacg aactagaatc gacgttgtag
      301 cgtagatata aaaattaata ctacatagaa atttaactaa gatttaagct actagttttt
      361 tgctagtagc ttttttattt tcttaatttt taaatgcctt tattttgaat tttaaggggg
      421 tatttagagc aaactctata tttatatatt tttatacctg tatatttata tatgtttctt
      481 aaaacgcaaa tatgagcaaa ataagggtac atctttctga catttaactt gtattcaaaa
      541 gacatattac ttaagttgct aaagtcattt taaatacaat cacttgtact ttttaaacat
      601 aagccagaaa aacgatataa cttttttata tatttttcta atttgattgg aactccctaa
      661 aatttatata acttaaaaag ttaaattgtt tatctcattt tttgtacata accagtggcg
      721 aatttttcag acactgccca tttacatgca aattaaaatt ttcatggtaa aactggtcaa
      781 ccatgccggt tgaacgctat agttcctgca ggggcaaaaa tacataaaaa aaacgctagc
      841 ttttaagcta acgtttcaaa tcttttaagt aataattttg tttttcaaaa ttccaaattg
      901 cagtaattga atgtgctttt tcttcttcgt ctgttgtttt atcttcgtca cttgtattaa
      961 ttaaattgcc atcttcggca tcgtctaaat ttaaaatttt atgtttttgt ttaagcaatc
     1021 caccataact caacatacgt tttcggtata aacctttttc taaatcattc acgatttcct
     1081 gatttctttc gtcatcatca gttaaaaaat cagatgattt aaccgaatat ttcgaggttt
     1141 ctttgattgc tgcttgaata tctttatcgc ctttttgatt tggtttgatc gctttaatat
     1201 ttgctactgg ccgataatta acttgtaatg ctttttgcca taaattaatc caatcatttt
     1261 gagaaatgta attctcagaa cctctaaaat atttactttc aacacacaat aaaacatgca
     1321 tatgttgatt ataactaccg tcatttttat taactgtaac ctctgtcgaa cgcataaaac
     1381 caattaaatt tttactaact tttttatatt taaataatct tctaaatgat tcagttaaat
     1441 gtttaagact tttttctaaa gtatctccat ctatcgcatt tcgtgttgat agtgtaagaa
     1501 acaaccaacg tgctttcggc ttttctttaa ccacttcttc aatcactctt tgagcttgat
     1561 aactattttt catagcacgc ctccaattac aaactggaca caattttgat ttacaaaacc
     1621 atgtcttatg taacttcaaa taaccttcat cagtaggctt gaactctaag acgttaccac
     1681 attgttttac gttaaaagcc tttttaattt ttaagatttc tagtatatct gcataactta
     1741 cattatcgat tttcttttct ctccacgggc gttgtttccc acttttcgtt aagtctttta
     1801 gtgttttatt atcttgattt tcatctatat attttgtatg attgtcttgc atataaaaag
     1861 acctccagta tttgatttta gctcgacact ttaatcttat tactggagtt tttttatgtc
     1921 aaattttatt ttatacacta aaaatctttc aaaacactgt tacatcaagc ttgaaacaac
     1981 attttaaaaa agaacgttat ttctatatag tatcaagaca agaagaaact cgttttcaac
     2041 tcgttttaaa accaccaacc aaaaccttaa aactcatcgt aatttcactt aagtttttct
     2101 taattattcc ttctttttct acgtcactta aattccacgt taaaggcgtc atatcaagta
     2161 atttattcaa ttcttcttta tttaatttaa ccttataact aatgtctatc atttcaattt
     2221 tattaaaatt agcttcaaat aaatctataa cctcagaatt atcataatcc ttatttttat
     2281 gattactaaa gactatattc cttatttctt ttaaataatc actattagga attattttta
     2341 tcaatttccc ctctttatct agaactcttt taaactcatc ataattagaa gggctaagca
     2401 cattaattaa aaagtctaca ttcccattca taactggtat atcagctaga tcagcaacca
     2461 accaatttat atttttatat gattttgctg ctttttttac accttctttt gcaatgtcta
     2521 tacctaagaa ttcattttca ccaagaaatg ttttactaat ttcatttaaa atataaccct
     2581 caccagtacc aacatctatc acattatttt tagttttaat ctcatatttt ttaataattt
     2641 ctatgatttt tgaaattaat atcgaataca aattgctact atcattcaat tctcttctag
     2701 caataaacaa ttttttatcg tatttacttt ttttatgccc tgaaattaaa ttcgaataac
     2761 cctcttttgc aatatcaaaa gtatggttat tctcacatgc taaagtttta taacctctct
     2821 ttaatttttc atcacatata gaacacttaa aaaacatatt cacacctcca atcaaataat
     2881 tataaataac aaagttgctt tattccaatt gctttattga cgttgagcct cggaaccctt
     2941 aacaatccca aaacttgtcg aatggtcggc ttaatagctc acgctatgcc ga
//
