LOCUS       NZ_CP013624             2216 bp    DNA     circular CON 06-MAR-2024
DEFINITION  Staphylococcus aureus strain RIVM1076 plasmid pRIVM1076, complete
            sequence.
ACCESSION   NZ_CP013624
VERSION     NZ_CP013624.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04325946
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Bosch,T., Witteveen,S., Haenen,A., Landman,F. and Schouls,L.M.
  TITLE     Next-Generation Sequencing Confirms Presumed Nosocomial Transmission
            of Livestock-Associated Methicillin-Resistant Staphylococcus aureus
            in the Netherlands
  JOURNAL   Appl Environ Microbiol 82 (14), 4081-4089 (2016)
   PUBMED   27129960
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Witteveen,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2015) Laboratory for Infectious Diseases &
            Screening, National Institute for Public Health & the Environment
            (RIVM), Antonie van Leeuwenhoeklaan 9, Bilthoven 3721 MA, The
            Netherlands
COMMENT     ##Assembly-Data-START##
            Assembly Method :: CLC Genomics Workbench v. version 8.5.1
            Sequencing Technology        :: 454; Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 03/06/2024 01:44:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 2
            CDSs (total)                 :: 2
            Genes (coding)               :: 2
            CDSs (with protein)          :: 2
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP013624.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="RIVM1076"
                     /isolation_source="unavailable"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pRIVM1076"
                     /geo_loc_name="Netherlands: Bilthoven"
                     /lat_lon="52.13 N 5.210 E"
                     /collection_date="2009-03-10"
                     /collected_by="Dutch National Institute for Public Health
                     and the Environment (RIVM)"
     gene            join(1841..2216,1..629)
                     /locus_tag="AUC52_RS00005"
                     /old_locus_tag="AUC52_00005"
     CDS             join(1841..2216,1..629)
                     /locus_tag="AUC52_RS00005"
                     /old_locus_tag="AUC52_00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_078098897.1"
                     /translation="MHSYTTRCIDENQDNETLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNVLEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQRVI
                     EEVVKEKPKARWLFLTLSTRNAIDGDHLEQSLKHMSKAFNKLKMYTKVKKNLIGFLRST
                     EVTVNKNDGSYNQHMHVLLCVESAYFKKQGQYIKQSEWVDLWQKALQVDYRPVANIKAI
                     KPNQKGDKDIQAAIKETSKYSVKSSDFLTDDDERNQEIVNDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDAEDGNLINTSDEDKTTDEEEKAHSITAIWNFEKQNYYLKDLKR"
                     /name="protein rep"
     gene            complement(1241..1564)
                     /gene="qacG"
                     /locus_tag="AUC52_RS00010"
                     /old_locus_tag="AUC52_00015"
     ARG             complement(1245..1564)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacG"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2..629
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.323"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1842..2216
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.415"
                     /coverage="100.0"
                     /evalue="3.3e-162"
ORIGIN
        1 caatagatgg tgaccattta gaacaaagtt taaaacatat gtctaaggct tttaataaat
       61 taaaaatgta tacaaaagtt aaaaagaatt taattggatt tttacgttca accgaagtaa
      121 cggttaataa aaatgacggt agttataatc aacatatgca tgttttatta tgtgttgaaa
      181 gtgcgtattt taaaaagcaa ggtcagtata taaaacaaag tgagtgggtc gatttatggc
      241 aaaaagcatt gcaagttgat tatcgaccag tcgcaaatat caaagcgatc aaaccgaatc
      301 aaaaaggcga taaagatatt caagcagcta tcaaagagac ttcaaaatac tcggttaagt
      361 catctgattt tttaactgat gatgatgaaa gaaatcaaga aatcgtgaat gatttggaaa
      421 aaggtttata ccgaaaacgt atgttgagtt atggtggatt gcttaaacaa aaacataaaa
      481 ttttaaattt agacgatgcc gaagatggca atttgattaa tacaagtgac gaagataaaa
      541 caacagacga agaagaaaaa gcacactcaa ttacggcaat ttggaatttt gaaaaacaaa
      601 attattactt aaaagatttg aaacgttagc ttaaaagcta gcgttttttt atgctttttt
      661 gcccctgcgg gaactatatc gttcaacctg tatggttgac cagtttttat gccaattttt
      721 aaatttgcat gtaactgggc agtgtctaaa aaattagtca ctggttttgc tcaaattttt
      781 gtttgtggaa ttagaatata tttatttggc tcatatttgc gttttaaaag cttgtgtagg
      841 tttttaggta taaaactata tgatttaccc ttaaatcttt aaaatgcccc cttagaattc
      901 aaaataaagg tatttaaaat ttggtatctt tgaaaattta aaaataaaaa atgctaagtg
      961 caacaataaa aaagttgtac ttagcaataa cacaaataat agaaaagcta attaatttaa
     1021 tttttgagta ctatattttt cctactcaat ttgcttaatt ataacataat taaagcatgc
     1081 atttatgccg agaaaattta ttgatgttga gaagaaccct taactaaact tgcagacgaa
     1141 tgtcggcata gcgtgagcta ttaagccgat cattcgacaa gttttgggat tgttaagggt
     1201 tccgaggctc aacgtcaata aagcaattgg aataaaataa tcaatggctt tctccaaata
     1261 catttaagag cactacacct ataacaatta agccaataga aattatacta attaaattaa
     1321 cattttcttt aaaaacgata actgagatta ttgttgttaa tactaatcct agacctgccc
     1381 atgttgcata agttatattt aagggcaaaa attttattgt taaacttaaa aaataaaagc
     1441 aaattccaaa cgaaagtagt gtacctaatg ttggccataa ctttgtgaaa ccctctgatg
     1501 tttttaaaaa actagttccg attatttctg ttgcaattga gataaataaa tataaataat
     1561 gcattaagct tctcctttaa tttctatttg atagcttata ttttaactta tattaagtga
     1621 aagaaaaatt tttaagattt gtattttaaa acgagttgaa aacgagtttc ttcttgtctt
     1681 gatactatat agaaataatt caaagatata aaaagtcgtt tgaattttga tattacagcg
     1741 tttttagcta tttttaacgt ggaaaataaa atttgacata aaaaaactcc agtaataaga
     1801 ttaaattgtc gagataaaat caaatactgg aggtttttat atgcattctt atactactag
     1861 atgtatagac gaaaatcaag ataacgaaac attaaaagat atgacgaaaa gtgggaaaca
     1921 acgcccatgg agagagaaga aaattgataa tgtaagttat gcagatatac tagaaatctt
     1981 aaaaattaaa aaagctttta atgtaaaaca atgtggtaac gtcttagagt tcaagccaac
     2041 tgatgaaggt tatttaaaat tacataagac atggttttgt aagtcaaaat tatgtccagt
     2101 ttgtaattgg aggcgtgcta tgaaaaatag ttatcaagct caaagagtga ttgaagaagt
     2161 ggtaaaagaa aagccgaaag cacgttggtt atttctcaca ttatcaacac gaaatg
//
