LOCUS       NZ_CP012991             3223 bp    DNA     circular CON 25-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KpN01 plasmid pKpN01-COL, complete
            sequence.
ACCESSION   NZ_CP012991
VERSION     NZ_CP012991.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04168649
            Assembly: GCF_001456055.3
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Lynch,T., Chen,L., Peirano,G., Gregson,D., Church,D., Conly,J.,
            Kreiswirth,B. and Pitout,J.
  TITLE     Molecular characterization and evolution of plasmids from blaNDM-7
            harbouring Klebsiella pneumoniae ST278 involved in nosocomial
            transmission
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Lynch,T., Chen,L., Peirano,G., Gregson,D., Church,D., Conly,J.,
            Kreiswirth,B. and Pitout,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2015) Microbiology, Calgary Laboratory Services,
            #9 3535 Research Road NW, Calgary, Alberta T2L 2K8, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT pipeline v. January 2015
            Genome Coverage                   :: 75x
            Sequencing Technology             :: Pacific Biosciences
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/25/2023 01:38:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,664
            CDSs (total)                      :: 5,540
            Genes (coding)                    :: 5,383
            CDSs (with protein)               :: 5,383
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 55 of 157
            Pseudo Genes (incomplete)         :: 101 of 157
            Pseudo Genes (internal stop)      :: 23 of 157
            Pseudo Genes (multiple problems)  :: 21 of 157
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP012991.1.
            Bacteria and source DNA available from Johann Pitout, Calgary
            Laboratory Services (Johann.Pitout@cls.ab.ca).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KpN01"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKpN01-COL"
                     /geo_loc_name="Canada: Calgary"
                     /lat_lon="51.01980214 N 114.05508041 W"
                     /collection_date="2013-06-24"
                     /collected_by="CLS"
     gene            551..1036
                     /locus_tag="AQD68_RS32250"
                     /old_locus_tag="AQD68_28535"
     CDS             551..1036
                     /locus_tag="AQD68_RS32250"
                     /old_locus_tag="AQD68_28535"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1040..1255
                     /locus_tag="AQD68_RS32255"
                     /old_locus_tag="AQD68_28695"
     CDS             1040..1255
                     /locus_tag="AQD68_RS32255"
                     /old_locus_tag="AQD68_28695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1423..1755
                     /locus_tag="AQD68_RS28535"
     CDS             1423..1755
                     /locus_tag="AQD68_RS28535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            2773..3096
                     /locus_tag="AQD68_RS28545"
                     /old_locus_tag="AQD68_28550"
     CDS             2773..3096
                     /locus_tag="AQD68_RS28545"
                     /old_locus_tag="AQD68_28550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             1424..1755
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          2486..2677
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.821"
                     /coverage="89.0"
                     /evalue="7.28e-73"
     Typing          complement(2023..2503)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.867"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          2..1353
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.832"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2774..3093
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.196"
                     /coverage="100.0"
                     /evalue="8.510000000000001e-64"
ORIGIN
        1 tcgccctacg ccaaaaagta cacttccggc gttctgtctt ttgccgaaca ggatttaccg
       61 cccggccagc gcgaaaagct gatggcgagt ttcgagcggg ttctgatgcc cggactcgat
      121 aaagaccagt acagcgtgct gtgggttgag caccgggaca aggggcggct ggagctgaac
      181 ttcctgatcc cgaacacgga gcttctgacc ggcaagcggc tacagccgta ttacgaccgc
      241 gccgaccgtc cgcgcatcga tgcctggcag accatagtga acggcaggct ggggctgcat
      301 gacccgaacg cgccggagaa ccggcgggtg ctggtctcgc cttccgcgct gccggaagcg
      361 aagcaggaag ccgcccaggc gattacgagc ggcctgcttg cccttgcctc atccggggag
      421 ctgaaaacgc gtcaggacgt cactgaggcg ctggaaagcg caggttttga ggtggtgcgc
      481 accacaaaaa gcagcatcag cattgccgac ccggacgggg ggcgaaacat ccgactgaag
      541 ggagccatct atgaacagtc ttttaacgct ggcgaaggac ttagagcaga aatcgaaagc
      601 gcagcagcag actaccggcg agatgctgaa agccgcattc agcgagcacg agaagtctgt
      661 cagagcggaa ctgagcgaaa gcgagaagag aatcagcgcc gccatcctcg accacgaccg
      721 gaagctgtcc tcagccatga gccagcgcac gaaagggatg ctgcgcatgg tcagccagac
      781 gtggctgacc atcgttctgg tctccgcgct gctgatagcg tcgagcgcgg gcattctgtg
      841 gtggcagggg cagcagatac tcgagaacta tacgaccatc cgggagcaga agagcacgca
      901 ggccatgctg tcagagagga acagcggcgt acagctctcg acctgcggcg agcagagacg
      961 ccgctgcgtg agggtgaacc cggaagcggg acagttcgga gaggactcga gctggatgat
     1021 actggcgggg aaatagcaca tgacggagct ggaaaaacag ttgctgagcg cattagagca
     1081 gctacagcag gactactcga aaaggctgga cgagtgggag aacgccttcg cggaatggcg
     1141 gacgatgtct ggtcttattc aacgggagaa cgcggcgctg aacgagcgcg tcacggtctt
     1201 gagcaggcag gtgcagagtt tgagcgagca gctgcgccgg ttgtcgaaag gctgaacgcg
     1261 atagaaactc accggcagca ggagcgggcg gtgcagcacc agaaagcgct ggagctggaa
     1321 cgatcgcagt ggcagcatca tggtccttcg ctatgataag ccgggttcta ttaattagtt
     1381 gttatgtaaa gatatttaaa tttttcaaca tgaggtattt ttatgaatac atatattttc
     1441 ctgggagggg ctattgtggc tgaagtcata ggcaccacct ttatgaaata ttcagaagga
     1501 tttacacgcc tgtggccgtc tgtaggaact gcaataagtt attgcgctgc cttttatctg
     1561 ctttcccaaa cattggaaaa tatacctacc ggtattgctt acgcaatttg gtctggtgcg
     1621 ggaattgtac taataagcct agctggttgg ttggttagcg gtcaaaaact tgattggccc
     1681 gcaattgcag gaatgttcct tatttgcgtt ggagttttgg ttattaatat tttttcaaaa
     1741 tcttctgcgc attaactttt taccgaagca tattggccaa agcaaaagga tttccttgag
     1801 atcctttctt tctgcgcgta atctttttcc ctgtaaacaa aaaaaccacc gctaccaacg
     1861 gtggttcgtt tgccggaatt cagagctacc aactctttgt tccgatggta actggctttc
     1921 acggagcgca gatacctaaa ctgtcctttc agtgtagccg cactcaggct ttcacttcaa
     1981 gaactctgta ggcattctct acaatctctg ctccgtgaac ccagttacca atggctaccg
     2041 ccactggcga tttgtcgtgt ctttccgggt tggactcaag ttgatagtta ccggaacggg
     2101 cgtggtagtc ggactgaacg gggggttcgt gcatacagtc catcctggag cgaactgcct
     2161 acccggaacc gagtttcagg cgtggaatga gaaaccgcgg ccataacagc ggaatgacac
     2221 cggtaaaccg aaaggcagga atgcggggga gcacgaggga gccaccaggg ggaaacgcct
     2281 ggtatcttta agccgcatcg ggtttcgcca ccgctgattt gagcgtcatt ttcgtgatac
     2341 tcgtcagggg ggcggagcct atggaaaaac ggcaactccg catccttttc ttggcctttc
     2401 ctgcttcgag cggcatttgc ttatcccgta atctgtgagc tggtaacgcc cgccgcagtc
     2461 tcacggcagg agcgtagcga ccgagtgagc gaggaggcgt catttcatca ccacctgcat
     2521 tttgcactta cgcccggcag ccgcattttt cccctgtcac atgaagcact ttcataaatt
     2581 accactccgt cataacatgt ttagccagta tacactccgc tagcgctacg tgactggttc
     2641 agggctgcgc cccgaaaccc gctaaaacca ctgccgcgtc tgcggcttgt ccaacaccgt
     2701 gccgaccggg aaaagatttc ccgtccgtcc cggcgttatc aggaggccgg attggcagac
     2761 aaacgcagca aaatgctcac catgtgggtg actgaggacg agcaccggcg tctgctggag
     2821 cgctgcgagg gtaagcaact cgcggcctgg atgcggcaga cgtgcctgga cgagaagccc
     2881 gcacgcgccg gcaaacttcc gtcgatctcg ccggcgctgc ttcgtcagct tgccggcatg
     2941 gggaacaacc ttaaccagat tgcccgccag gttaacgccg gtggtggaag cggacacgac
     3001 cgcgtgcaga ttgtcgccgc gctgatggcc atcgatgccg gactcgagcg gttgcggcat
     3061 gccgtactgg aaaagggtgc tgatgatgat cgttaagttt catcctcggg ggcgcggcgg
     3121 cggcggcggg ccggtcgatt atctgctggg taaagatcgc cagcgcgacg gcgccagcgt
     3181 tctgcagggg aagccggatg aagtccggga gcttatcgac gcc
//
