LOCUS       NZ_CP012755             6141 bp    DNA     circular CON 03-MAR-2024
DEFINITION  Klebsiella pneumoniae strain KP617 plasmid KP-p2, complete sequence.
ACCESSION   NZ_CP012755
VERSION     NZ_CP012755.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04044480
            Assembly: GCF_001307175.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Kwon,T., Yang,J.W., Lee,S., Yun,M.R., Yoo,W.G., Kim,H.S., Cha,J.O.
            and Kim,D.W.
  TITLE     Complete Genome Sequence of Klebsiella pneumoniae subsp. pneumoniae
            KP617, Coproducing OXA-232 and NDM-1 Carbapenemases, Isolated in
            South Korea
  JOURNAL   Genome Announc 4 (1), e01550-15 (2016)
   PUBMED   26769936
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Kwon,T., Kim,D.-W., Cha,J.-O., Yang,J.-W., Kim,H.-S. and Park,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-SEP-2015) Division of Biosafety Evaluation & Control,
            Korea National Institute of Health, Korea Centers for Disease
            Control & Prevention, 187 Osongsaengmyeong 2-ro, Osong, Heungdeok,
            Cheongju, Chungbuk 363-951, Korea, Republic of
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/03/2024 07:31:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,555
            CDSs (total)                      :: 5,432
            Genes (coding)                    :: 5,299
            CDSs (with protein)               :: 5,299
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 55 of 133
            Pseudo Genes (incomplete)         :: 77 of 133
            Pseudo Genes (internal stop)      :: 22 of 133
            Pseudo Genes (multiple problems)  :: 18 of 133
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP012755.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP617"
                     /isolation_source="Burn Patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="KP-p2"
                     /geo_loc_name="Korea: Seoul"
                     /lat_lon="37.6463 N 127.0288 E"
                     /collection_date="2013-05-29"
                     /collected_by="KEPCO Medical Center"
     gene            join(5610..6141,1..416)
                     /locus_tag="AN966_RS27945"
                     /old_locus_tag="AN966_27875"
     CDS             join(5610..6141,1..416)
                     /locus_tag="AN966_RS27945"
                     /old_locus_tag="AN966_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     gene            824..1216
                     /locus_tag="AN966_RS27950"
                     /old_locus_tag="AN966_27880"
     CDS             824..1216
                     /locus_tag="AN966_RS27950"
                     /old_locus_tag="AN966_27880"
                     /inference="COORDINATES: protein motif:HMM:NF037437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            1198..1536
                     /locus_tag="AN966_RS27955"
                     /old_locus_tag="AN966_27885"
     CDS             1198..1536
                     /locus_tag="AN966_RS27955"
                     /old_locus_tag="AN966_27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1517..2971
                     /locus_tag="AN966_RS31400"
     CDS             1517..2971
                     /locus_tag="AN966_RS31400"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3253..3534)
                     /locus_tag="AN966_RS27975"
                     /old_locus_tag="AN966_27905"
     CDS             complement(3253..3534)
                     /locus_tag="AN966_RS27975"
                     /old_locus_tag="AN966_27905"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            3878..4675
                     /locus_tag="AN966_RS27985"
                     /old_locus_tag="AN966_27915"
     CDS             3878..4675
                     /locus_tag="AN966_RS27985"
                     /old_locus_tag="AN966_27915"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /protein_id="WP_043907054.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            complement(5082..>5234)
                     /locus_tag="AN966_RS31045"
                     /pseudo=""
     CDS             complement(5082..>5234)
                     /locus_tag="AN966_RS31045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     ARG             3879..4672
                     /name="blaOXA-232"
                     /gene="blaOXA-232"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          138..416
                     /name="ColKP3"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.87e-144"
     Typing          1518..2968
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 actgtaccct gttcgagaaa acgcgcctgt gggcctataa agcgatccgg cagggctggc
       61 cggattaccc cgaatggttg gccgcttgtg ttgaccgggc atcaggatat aacgcccagt
      121 tcgagcagcc gctccccgca aacgaagtcc ggcacacggc caagtccatt gccaaatgga
      181 cgcaccagca cctaagcccc gcagggttca gagaggagca ggcccggaga ggtgcgaagg
      241 gcggcaaggt atccaaggga gggggaaggc cgagcaatag cggtaaggac aagtccgatc
      301 tattgccgga ggttctgcgg cttaaggctc aggggtacac caaccgcgat atagcggacg
      361 atcttcaaat ctcccccagt acggtgagcg tttacctaaa gcgtgatcgt ccgtgatcga
      421 aacaagcctt atcagataat agcccccttt aggggggttc ctgcgctctt gtcttttagc
      481 cttaaaaaat ccttttagcg cggatctttg gcgaggtgga caactgcgcc tgtccgtcca
      541 tcgcccccct gttttttcgg ggggtgtggg cggcagcagt tatccacgga gccgctcttt
      601 cccttgatct tgctctaggg cttgcagtat cccgcccatg gttgaataat gtactaagca
      661 aggctacaca gaggcccctc tacctgcttc gcggtctcgg gcctctgtgg ccttgttcag
      721 gggtgctacg cccccccgaa accccctgtg gcccccgctc gatccgcccc ttcgggacgg
      781 tctgcgcggt ggcttcttgg ctctggagaa tggacgaggc cctatgtcaa caacacagat
      841 agagaaagat tcgagaagga aggcagtcaa tggcgttatt tctagtttcc aactagaggg
      901 ggaggccata accgatgaat tcaaaattct acttgaagat tatatcaatg gaaaaatttc
      961 caccgatgag gtgttagcta tggtaagtga cgaacccaca acaacggaat acgactacga
     1021 aatcaccccc cgcactctcg gtcttggcgg gggctggaat ctgcgtttgc tggagaacgg
     1081 cgaagaggtc ggcgggggtg ttttcccctt gccggagcac tgcgattttc gggatgaaaa
     1141 ggcgctccag acgctactcg atagcctcta tgaggacgcc ttagcggagg cctctgcatg
     1201 gctggccagc cgttaaagcg cctccgctct atcaagatcc gggtctcaga tgcggagctt
     1261 gagcgcctgc gggagctctg ccccaaggcc caactggcgg agtggatgcg ggagcaatgt
     1321 ctcggtgttg cgcaacccca gcgccgaacg ccggcgccta ccgtagatcc ggcgctcttg
     1381 cggcagttgg ccggaatggg caacaacctg aaccagatcg cccggcgcgt taattccggg
     1441 gagtgggggc cgttggatcg gctgcgcatc atagcggagc tgtccgctat aggccgcgag
     1501 ctggaggaac tgcaccatga tcatcaaatt ccataaccgt ggggtaggcc ggggctccgg
     1561 gcctgttggc tacttgttgg gccgtgacgg gaaccgggaa ggcgcaacgc tcgaccgggg
     1621 caaccctgcg gtgattgaag ccctgattga ttccagtccc tacgccaaaa aatacacctc
     1681 tggcgttctg tcgtttgccg aggctgattt acccagggcg accaaggaca agctgatgag
     1741 cgactttgaa aaggcgctcc tgcccggact ggagggcaac cagtacgctg ttttgtgggt
     1801 agagcaccgc gacaaggggc ggctggagct gaattttgta gtcccgaaca tcgaattgca
     1861 gagcgggaag cggctgcaac cctacttcca caaggccgac aatccgcgcc tcgatgcgtg
     1921 gcgaaccgtg gtaaacgggg agctgaaatt gcacgacccg gacgatccgt taaaccgtca
     1981 ggccgttatc acgccaaagg atttgccgag ggggcacaag gaggccgctc aggccattac
     2041 agacgggctt ttacgcatgg ccggagcagg ggagatccag agccgcgatg atgtgcgcaa
     2101 agccttgcag ggtgcgggtt tcagcgtggt acgcgaaacc aaaaacagcc tgagcattgc
     2161 cgacccggac ggggggcgga acatccgatt aaagggggtg ctgtatgagc aagattttag
     2221 acatggcgaa ggacttcgag cagagctcga agcagcaggc caacgctatc gagaagcgtc
     2281 tgaccgaaga attcacgagg cacgagaaag ctatctccgg ggcgttgaaa tcaagcgagg
     2341 agaaaatgag cgccgctatt gccgagcaca gccagaaaat gggctggctg ttgctcaaaa
     2401 cgtggatctg gatgctggtc agcatcgtcc tgttcttgag cgcaacgagc gggatcctct
     2461 ggtatcaggg ggaattgata gcggagcggc aagccactct ccaagccttg gcagagaagg
     2521 gcggcaagct aaagctgacc cagtgcggga agaaattgga gcacctttgc gtgcagatct
     2581 ccgaccccaa tccaaccgtt tgggggaagg atacctattg gatcgtgccg aaggggtact
     2641 aaatgacggg attggagcgg gaattgctct gcgcgttcga ggctttgcag agcgcatacg
     2701 agcagcagca caaggcatgg caggaaagct acgcgaactt ggcgagcatg tacgagagca
     2761 ccaaacaaga ggccgagatc agcagggcag cagcagagcg attgacgagg caggtgaacg
     2821 gcttaacgca gcaagtggac ggcttgagca agcagctgga gggcttgacc gggctagtca
     2881 gcaagttgca aaagaaaggg aacacaggct cctgaaggca ctggatcgag gctgggagcg
     2941 tggagacaga tcgcgaggct ttgagcgttg acggaacaaa acggccgtca gttataaaaa
     3001 aaggaaagtg ctatcacttt ccttttatcg gcgggaatta tcgttgagtt cttctatgcc
     3061 atttttcata gtagtaactg taatcttcat ctctaccacc agattcaatc cagtcatcat
     3121 actcagggct atttttgaaa tctgaatcag acatgctaac tccttgttat tagttggctt
     3181 ctatggaagc tagcgcaccg tgaagttatg atcaacgatg tagtccacat cattgtcgaa
     3241 atgtgcgttc agctagtctt cggtgttttc tgctttattt ctagcggcga ttctcgcgtc
     3301 tgcccatgcg gcagactttt tagcctgttc tcgttgttct tcggttgaag actcgctgtt
     3361 gagaactttg ttgctcgttt gtttgaccac cgaatccaaa cctgttgttg ctgcaaaatg
     3421 agctaggtta acaactccct tacctaacaa cttggcacta gcgattgcgg tttctttagc
     3481 cccaaaagca tcagacacct cctcaagcgc tttagatagg ttaaaaaatc ccatactccc
     3541 cctaagaata tcatcaataa aattgagtgt tgctctgtgg ataacttgca gagtttatta
     3601 agtatcattg cagcaaagat gaaatcaatg atttatcaaa aatgattgaa aggtggttgt
     3661 aaataatgtt acaatgtgtg agaagcagtc taaattcttc gtgaaatagt gatttttgaa
     3721 gctaataaaa aacacacgtg gaatttaggt aatagttaaa gcttgtgcct gattgttgcc
     3781 acaattggtc agcagtttgc tagggaatga gagggcagag aatgataagc ttagcactta
     3841 atttgtatgg atatattgca ttaagcaagg ggacgttatg cgtgtattag ccttatcggc
     3901 tgtgtttttg gtggcatcga ttatcggaat gccagcggta gcaaaggaat ggcaagaaaa
     3961 caaaagttgg aatgctcact ttactgaaca taaatcacag ggcgtagttg tgctctggaa
     4021 tgagaataag cagcaaggat ttaccaataa tcttaaacgg gcgaaccaag catttttacc
     4081 cgcatctacc tttaaaattc ccaatagctt gatcgccctc gatttgggcg tggttaagga
     4141 tgaacaccaa gtctttaagt gggatggaca gacgcgtgat atcgccgctt ggaatcgtga
     4201 ccatgactta attaccgcga tgaagtactc agttgtgcct gtttatcaag aatttgcccg
     4261 ccaaattggt gaggcacgta tgagtaaaat gctgcacgcc ttcgattatg gcaatgagga
     4321 tatctcgggc aatgtagaca gtttttggct cgatggtggt attcgcattt cggctaccca
     4381 gcaaatcgct tttttacgca agctgtatca caacaagctg cacgtttctg agcgtagtca
     4441 gcgcatcgtg aaacaagcca tgctgaccga agccaatggc gactatatta ttcgggctaa
     4501 aacgggatac tcgactagta tcgaacctaa gattggctgg tgggttggtt gggttgaact
     4561 tgatgataat gtgtggtttt ttgcgatgaa tatggatatg cccacatcgg atggtttagg
     4621 gctgcgccaa gccatcacaa aagaagtgct caaacaggag aaaattattc cctagaagtg
     4681 gttagcgcgt atttgtgtga aatagccgtc atataagctg taaagatata tggacaaaat
     4741 gctgatgctc gatgcgcttg tagttaatgg ctcatactta atcggtcagg cctaatcgac
     4801 ttaagctccg cgcgttaact ttcagcctga gcattagcat cacattccta aacgtaaaaa
     4861 gcctagccaa tttgctaggc ttttttgtta tctcaatggc catggtcact caacatgggg
     4921 gtgtgaataa aggagtggcg atgtcagtaa aggtgaatgc cgctgcgata aagtgcctag
     4981 cccatcaagg attgtaggct ttcttggctg ctaaagcgtt tgacgaaaaa gtccaagaag
     5041 gcgctgatat tggttctcca tcattttcat ctaccggttt actataggtc aaccaggccg
     5101 tccttgccgg cgcttgggac gcagacgatg gcatcaaatg cctcgctcaa attcgtttct
     5161 acagaggcgc tttgggaccg cattcgcttt gctttcatgg gggcatctgt caatgtcaga
     5221 catgaatcct ccgatatagg ttgatttttg atctcttcaa cacttggtgt tgtccgacta
     5281 gagagactta tatggatcag tattcttgca ctgacaatgg tgccactcaa tcccaaggat
     5341 cgacctagtt agaggcgtag atcaagcaat tcaaaacagc gcagaagcag aaggaggtgg
     5401 aaatatgttc cagggtgaaa ggttacatgc aactaacgtt gctctctagg atgtgcctgc
     5461 tgtaacgtat tgtccagaaa agacaacccc ccgttatcgc ttgcatcttg gcggagctgc
     5521 gaaaaacggg ggccgtgacg aatcacaaga gaagtctatc acatggatca tgccaaacaa
     5581 ccccacgccg ccaataagcg cggtggtgct tgagaaagtg cgcacagctt gagctgtttc
     5641 gggagcgcct gccccggaaa ccctactatt ccgacgagct tacaacgggc ctacggattg
     5701 cggatgttgc ccgcgccctt ggtgcgcgtt acatccagcc gaacgggcca acgcaccgcc
     5761 actggatcgt ttttgacgta gaccatgcag ccgctacgtt atcttgggat gacgtaggcg
     5821 ctccagcccc taacattacc gtcacgaaca aggccaacgg ccacgcgcat ctgatctacg
     5881 gactggatac gccgatccgc actgccccgg atggaaatgc agcccctctg cggtatgccg
     5941 ccgccatcga ggccgcgctc cgggagaaat taggggcgga tatgggctac tccgggctga
     6001 tctgcaagaa ccccctgcat gagcactggc tggtgcaggt atgggaaccc cggctctacg
     6061 atttagcctg gctatccgat tacctcgatc tgagctccta caatggccgc aaatcgcttc
     6121 ctgagtacgg gcttgggcga a
//
