LOCUS       NZ_CP012628             6647 bp    DNA     circular CON 11-JUN-2024
DEFINITION  Escherichia coli strain SF-468 plasmid pSF-468-3, complete sequence.
ACCESSION   NZ_CP012628
VERSION     NZ_CP012628.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04026228
            Assembly: GCF_001280345.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Stephens,C.M., Skerker,J.M., Sekhon,M.S., Arkin,A.P. and Riley,L.W.
  TITLE     Complete Genome Sequences of Four Escherichia coli ST95 Isolates
            from Bloodstream Infections
  JOURNAL   Genome Announc 3 (6), e01241-15 (2015)
   PUBMED   26543109
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Stephens,C.M., Skerker,J., Sekhon,M., Arkin,A. and Riley,L.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2015) Biology, Santa Clara University, 500 El
            Camino Real, Santa Clara, CA 95053, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Coverage                   :: 90X
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001280345.1-RS_2024_06_11
            Annotation Date                   :: 06/11/2024 02:21:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,367
            CDSs (total)                      :: 5,248
            Genes (coding)                    :: 5,017
            CDSs (with protein)               :: 5,017
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 98 of 231
            Pseudo Genes (incomplete)         :: 137 of 231
            Pseudo Genes (internal stop)      :: 46 of 231
            Pseudo Genes (multiple problems)  :: 46 of 231
            Pseudo Genes (short protein)      :: 1 of 231
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP012628.1.
            Source DNA is available from: Craig Stephens, Biology Dept., Santa
            Clara University, Santa Clara, CA, 95053.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SF-468"
                     /isolation_source="patient blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSF-468-3"
                     /geo_loc_name="USA: San Francisco"
                     /lat_lon="37.755651 N 122.404572 W"
                     /collection_date="2010-04-05"
                     /collected_by="SFGH"
     gene            188..325
                     /locus_tag="AN206_RS27200"
                     /old_locus_tag="AN206_27290"
     CDS             188..325
                     /locus_tag="AN206_RS27200"
                     /old_locus_tag="AN206_27290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(524..682)
                     /locus_tag="AN206_RS31745"
     CDS             complement(524..682)
                     /locus_tag="AN206_RS31745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(1352..1543)
                     /locus_tag="AN206_RS27205"
                     /old_locus_tag="AN206_27295"
     CDS             complement(1352..1543)
                     /locus_tag="AN206_RS27205"
                     /old_locus_tag="AN206_27295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1607..1930
                     /locus_tag="AN206_RS27210"
                     /old_locus_tag="AN206_27300"
     CDS             1607..1930
                     /locus_tag="AN206_RS27210"
                     /old_locus_tag="AN206_27300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2139..>2570
                     /locus_tag="AN206_RS31565"
                     /pseudo=""
     CDS             2139..>2570
                     /locus_tag="AN206_RS31565"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2608..3126
                     /locus_tag="AN206_RS30740"
     CDS             2608..3126
                     /locus_tag="AN206_RS30740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            3133..3366
                     /locus_tag="AN206_RS30745"
     CDS             3133..3366
                     /locus_tag="AN206_RS30745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3684..4103)
                     /locus_tag="AN206_RS27225"
                     /old_locus_tag="AN206_27315"
     CDS             complement(3684..4103)
                     /locus_tag="AN206_RS27225"
                     /old_locus_tag="AN206_27315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(4184..4621)
                     /locus_tag="AN206_RS27230"
                     /old_locus_tag="AN206_27320"
     CDS             complement(4184..4621)
                     /locus_tag="AN206_RS27230"
                     /old_locus_tag="AN206_27320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            4881..6449
                     /gene="cea"
                     /locus_tag="AN206_RS27235"
                     /old_locus_tag="AN206_27325"
     CDS             4881..6449
                     /gene="cea"
                     /locus_tag="AN206_RS27235"
                     /old_locus_tag="AN206_27325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     Typing          1180..1267
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(611..1166)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.52"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1921..3470
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgcttatca ctattgcata tagcatctta tctgacacaa ggaaataata cccctcgctg
       61 ttttttgtta taaggtatat atatataagt gtgcagtaca ggccaaataa aatatttttt
      121 atgtagtatc ttaagctcat aaattaaacc tcgccatata ttcttttcat tttataagga
      181 tcgagttatg aggaaaagat tttttgtggg aatattcgcg ataaacctcc ttgttggatg
      241 tcaggctaac tatatacgtg atgttcaggg agggaccatc gcaccatcct cctcttctaa
      301 actgacgggg atcgcggttc agtagaaaag atcaaaggat cttcttgaga tccttttttt
      361 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccaacggt ggtttgtttg
      421 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
      481 ccaaatactg tccttctagt gtagccgtag tcgggccact acttcaagaa ctctgtagca
      541 ccgtttgtgc catcatcgct ctgctaatcc ggttaccagt ggctgctgcc agtggcgtta
      601 aggcgtgcct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
      661 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
      721 gataccaaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
      781 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
      841 acgcctggtg tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtctatttt
      901 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc ctgctacgcg gccttcttcc
      961 tgtttctggt cttttgctca catgttcttt ccggccttat cccctgattc tgtggataac
     1021 tgtgttaccg tttttgtgtg agtcagtacc gctcgccgca gtcgaacgac cgagcgtagc
     1081 gagtcagtga gcgaggaagc ggaaaagcgc ctggacgtgc attttctcct tacgcatctg
     1141 tgcggcattt cacacccggc atggcgtact tttcatacaa tccgcactga tgccgcatgg
     1201 ttaagccagt atacactccg ctatcgctac gtgactgggt cagggctgcg ccccgacacc
     1261 cgctaaaacc tgctgacgcg ccctgacggg cttgtcagct cccggcatcc gctcacagac
     1321 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tccccgaaac
     1381 gtgcgaggca gctgcggtaa agctcatcgg cgtggtcgtg aagcgattca caaatatcgg
     1441 cctgttcatc tgcgtccagt tcgttgagct tctccagcag cgttaatgtc tggcttctga
     1501 taaagcgggc catgttaagg gcggtttttt cctgtttagt cactgatgcc tccgtgtaag
     1561 ggggatttct gttcatgggg taatgatacc gatgaaacgc gagaggatgc tcacaatacg
     1621 ggttactgat gatgaacatg cccggttact tgaacgctgt gagggtaaac aactggcggt
     1681 atggatgcgg cgggtctgcc tgggggagcc ggttgcccgt tccggaaaac tgccgacact
     1741 ggcaccgccg ttactgcgtc agctggccgc catcggaaat aacctgaatc agacagcccg
     1801 taaggtgaac agcgggcagt ggtcttccgg tgaccgggtt caggtggtgg ccgcactgat
     1861 ggccatcggg gatgagctgc gccggctgcg tctggccgtc agggaacagg gggcgcggga
     1921 tgatagttaa atttcatgcc aggggaaaag gtggtggcag tggtccggtt gattacctgc
     1981 tggggaggga gcgtaaccgc gaaggcgcaa cggtgcttca gggtaatccg gaagaagtcc
     2041 gggaactcat cgatgccacg ccatttgcga agaaatacac gtccggtgtt ctgtcgttcg
     2101 cggagaagga gctgccgccg ggaggacgtg aaaaagtgat ggcgagcttt gagcgtgtac
     2161 tgatgcccgg tctcgaaaaa aatcagtaca gcatcctgtg ggtggagcac caggacaagg
     2221 gacggcttga gctgaatttt gtcattccga acatggagct acagaccgga aaacgcctcc
     2281 agccgtacta cgaccgcgca gacaggccta gaattgatgc ctggcagacg ctggtgaatc
     2341 accattacgg gctgcatgac ccgaacgccc cggagaaccg caggacgctg acactccctg
     2401 ataacctgcc tgaaacgaaa caggcgcttg ctgagggcgt cacgcgaggt atagatgcac
     2461 tttaccatgc cggagagata aaaggccgtc aggatgtgat tcaggcgctc actgaggcgg
     2521 ggctggaagt ggtcagggtg acgcgaagca gtatcagcat tgcagatccg aacggcggga
     2581 agaatatcag gctgaaagga gcattttatg agcaatcttt tgcagacggg cgcggagttc
     2641 gagaaaaagc tgaaagagag agccgaatct acagagaaaa tgctgaacaa cgagttcagg
     2701 aggctcggcg aatctgtaag cgaggctgtg acatcaaacg agacgaaaat cagagacgct
     2761 atagccctgt tcacagcctc gacagaggaa tcgctggaaa aacaccggga aggggtgaaa
     2821 gaggcgatga tgcagcacag gagggacgtg ttaaagctgg cagggaatac gggcatgatg
     2881 ttactgggga tagtctttct cctgtttacc gcgagtggcg ggacgctctg gtatcttgga
     2941 gggaggatac aggcgaacct ggaagaaatc aggaagcagg aagagacatt gcagaaactg
     3001 aacgcgaaga catggggcgt ggagtttgtg caggacggga acaggaaatt ccttgtcctt
     3061 ccgtacggga aatcagcgga ggtgattccc tttcagggga aagagtgggt acatctgaag
     3121 gagtgacaca gagtgacaga gctggaaaca catttgctga acgccttaga gcagctgcaa
     3181 caggactata tgcagcggct gagcgaatgg gagagcgcct tcgtggaatt gcagaagatg
     3241 ttttcgctta cgcaacggga caacgcgatg ctgaacgagc gggtcatgca gttgagtcag
     3301 caggtgcagc acttgagcga gcagacagaa cgcttgagcc agttatacag cgagaactgg
     3361 agataagaga ggaacggctg atacaggagc gcgaacatgt gttatccctg gaacgggagc
     3421 gtcagccgga aatacaggaa cgcacgctgg atggcccttc gctgggatgg tgaaaccatg
     3481 aaaaatggca gcttcagtgg attaagtggg ggtaatgtgg cctgtaccct ctggttgcat
     3541 aggtattcat acggttaaaa tttatcaggc gcgatcgcgg cagtttttcg ggtggtttgt
     3601 tgccattttt acctgtctgc tgccgtgatc gcgctgaacg cgttttagcg gtgcgtacaa
     3661 ttaagggatt atggtaaatc cacttactgt ctgccctcgt agccatcgag ataaaccgca
     3721 gaaatcgtgt cagccagcag cctggatttt ttcggggtaa ggtctttgct gcggtaatca
     3781 gtacccgcaa attgagttgt gccccggagt gagtttaaaa attcctggct gtgaaactgc
     3841 tgaatgcgtt gttgcacatc actccgtgat tttcctgtcg ctttatcttt agctcccata
     3901 tcccagaact gccgaaaaaa aggcaccatc attcgtgttg tgtcggcttt ttgggtggaa
     3961 aagttgggat caaaaccgtc atcagaagca tatacgtaat gccgggtatg ttgttccacg
     4021 ctgccctttg gggtacaggc tgtgagagct actgttgctg ttatcatggc gataagacaa
     4081 agtttgtttc cggtacgtct catgagtgtc tccctacctt aaagtattta ttctcggtga
     4141 gttattatcc gaagactatt ttatcatcag tctctgacag cgttcagcga ccgaacaacc
     4201 tggaccagaa tccccgtttc ttttccagtt ccagtgcctg cctgagctgg gctatctcat
     4261 tttgtagctg ttcccgttct gcttcctggc gtctgcgatc catatcctgt gcctgtttat
     4321 cctcaagcat cagcgtcagg cattgtttca gctcattcag ctcccggaga atgcgttctg
     4381 tctgctgatc atgtggattt tctgcatgtc cctcactgtg cctttctggt gtctcattct
     4441 gctttaattc gccgtatgcc cggatcagtt cactggtttc aaactcccgt cgtccgtccc
     4501 tgccggtacg gtaactgaca aggcctgcgc gcatatcacg gtaaaactgt gaacgcgatc
     4561 tgcctgtcat ttttagtgcg tcccggagtg tgtgccatgc cataaagtga cagtgtccca
     4621 tagatgtctc atctcatagt ttcagtaaaa cataatgagg tctgagaacg gtaatgtttg
     4681 tgctggtttt tgtggcatcg ggcgagaata gcgcgtggtg tgaaagactg tttttttgat
     4741 cgttttcaca aaaatggaag tccacagtct tgacagggaa aatgcagcgg cgtagctttt
     4801 atgctgtata taaaaccagt ggttatatgt acagtattta tttttaactt attgttttaa
     4861 aagtcaaaga ggattttata atggaaaccg cggtagcgta ctataaagat ggtgttcctt
     4921 atgatgataa gggacaggta attattactc ttttgaatgg tactcctgac gggagtggct
     4981 ctggcggcgg aggtggaaaa ggaggcagta aaagtgaaag ttctgcagct attcatgcaa
     5041 ctgctaaatg gtctactgct caattaaaga aaacacaggc agagcaggct gcccgggcaa
     5101 aagctgcagc ggaagcacag gcgaaagcaa aggcaaacag ggatgcgctg actcagcgcc
     5161 tgaaggatat cgtgaatgag gctcttcgtc acaatgcctc acgtacgcct tcagcaacag
     5221 agcttgctca tgctaataat gcagctatgc aggcggaagc agagcgtttg cgccttgcga
     5281 aagcagaaga aaaagcccgt aaagaagcgg aagcagcaga aaaggctttt caggaagcag
     5341 aacaacgacg taaagagatt gaacgggaga aggctgaaac agaacgccag ttgaaactgg
     5401 ctgaagctga agagaaacga ctggctgcat tgagtgaaga agctaaagct gttgagatcg
     5461 cccaaaaaaa actttctgct gcacaatctg aagtggtgaa aatggatgga gagattaaga
     5521 ctctcaattc tcgtttaagc tccagtatcc atgcccgtga tgcagaaatg aaaacgctcg
     5581 ctggaaaacg aaatgaactg gctcaggcat ccgctaaata taaagaactg gatgagctgg
     5641 tcaaaaaact atcaccaaga gccaatgatc cgcttcagaa ccgtcctttt tttgaagcaa
     5701 ccagacgacg ggttggggcc ggtaagatta gagaagaaaa acaaaaacag gtaacagcat
     5761 cagaaacacg tattaaccgg ataaatgctg atataactca gatccagaag gctatttctc
     5821 aggtcagtaa taatcgtaat gccggtatcg ctcgtgttca tgaagctgaa gaaaatttga
     5881 aaaaagcaca gaataatctc cttaattcac agattaagga tgctgttgat gcaacagtta
     5941 gcttttatca aacgctgact gaaaaatatg gtgaaaaata ttcgaaaatg gcacaggaac
     6001 ttgctgataa gtctaaaggt aagaaaatcg gcaatgtgaa tgaagctctc gctgcttttg
     6061 aaaaatacaa ggatgtttta aataagaaat tcagcaaagc cgatcgtgat gctattttta
     6121 atgcgttggc atcggtgaag tatgatgact gggctaaaca tttagatcag tttgccaagt
     6181 acttgaagat tacggggcat gtttcttttg gatatgatgt ggtatctgat atcctaaaaa
     6241 ttaaggatac aggtgactgg aagccactat ttcttacatt agagaagaaa gctgcagatg
     6301 caggggtgag ttatgttgtt gctttacttt ttagcttgct tgctggaact acattaggta
     6361 tttggggtat tgctattgtt acaggaattc tatgctccta tattgataag aataaactta
     6421 atactataaa tgaggtgtta gggatttaat tattctttat tgatataaaa agtcctagca
     6481 atccaaatgg gattgctagg accaaacaaa gtagattata tagcataaat aggtttaatt
     6541 ttgctacggg ggcgttattt aggttttttc ttctttcgaa aaaatctttc tttatgaagt
     6601 taaaagctat gtgttcaata gcatattttg aatatggaca tagaata
//
