LOCUS       NZ_CP012562             6141 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Klebsiella pneumoniae strain UCLAOXA232KP plasmid pUCLAOXA232-1,
            complete sequence.
ACCESSION   NZ_CP012562
VERSION     NZ_CP012562.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN04008892
            Assembly: GCF_001741665.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Yang,S.
  TITLE     Identification of Klebsiella pneumoniae Producing OXA-232, CTX-M-15
            and RmtF by Whole Genome Sequencing Analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Yang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2015) Department of Clinical Microbiology, UCLA
            Medical Center, 11633 San Vicente Blvd., Los Angeles, CA 90049, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2015
            Assembly Method                   :: a5-MiSeq v. March-2015
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/04/2024 13:46:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,907
            CDSs (total)                      :: 5,772
            Genes (coding)                    :: 5,588
            CDSs (with protein)               :: 5,588
            Genes (RNA)                       :: 135
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 99 of 184
            Pseudo Genes (incomplete)         :: 89 of 184
            Pseudo Genes (internal stop)      :: 16 of 184
            Pseudo Genes (multiple problems)  :: 20 of 184
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP012562.1.
            The source DNA and bacteria are available from UCLA Clinical
            Microbiology Laboratory, Los Angeles, CA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="UCLAOXA232KP"
                     /isolation_source="abdominal drainage"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pUCLAOXA232-1"
                     /geo_loc_name="USA: California"
                     /lat_lon="34.0561 N 118.4297 W"
                     /collection_date="2014-10-12"
                     /collected_by="UCLA"
     gene            join(6117..6141,1..368)
                     /locus_tag="AM277_RS27575"
                     /old_locus_tag="AM277_26495"
     CDS             join(6117..6141,1..368)
                     /locus_tag="AM277_RS27575"
                     /old_locus_tag="AM277_26495"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            350..688
                     /locus_tag="AM277_RS27580"
                     /old_locus_tag="AM277_26500"
     CDS             350..688
                     /locus_tag="AM277_RS27580"
                     /old_locus_tag="AM277_26500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            669..2123
                     /locus_tag="AM277_RS33030"
     CDS             669..2123
                     /locus_tag="AM277_RS33030"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2405..2686)
                     /locus_tag="AM277_RS27590"
                     /old_locus_tag="AM277_26520"
     CDS             complement(2405..2686)
                     /locus_tag="AM277_RS27590"
                     /old_locus_tag="AM277_26520"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            3030..3827
                     /locus_tag="AM277_RS27600"
                     /old_locus_tag="AM277_26530"
     CDS             3030..3827
                     /locus_tag="AM277_RS27600"
                     /old_locus_tag="AM277_26530"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /protein_id="WP_043907054.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            complement(4234..>4386)
                     /locus_tag="AM277_RS31975"
                     /pseudo=""
     CDS             complement(4234..>4386)
                     /locus_tag="AM277_RS31975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            4762..5709
                     /locus_tag="AM277_RS27610"
                     /old_locus_tag="AM277_26535"
     CDS             4762..5709
                     /locus_tag="AM277_RS27610"
                     /old_locus_tag="AM277_26535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     ARG             3031..3824
                     /name="blaOXA-232"
                     /gene="blaOXA-232"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          4775..5696
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          670..2120
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attcgagaag gaaggcagtc aatggcgtta tttctagttt ccaactagag ggggaggcca
       61 taaccgatga attcaaaatt ctacttgaag attatatcaa tggaaaaatt tccaccgatg
      121 aggtgttagc tatggtaagt gacgaaccca caacaacgga atacgactac gaaatcaccc
      181 cccgcactct cggtcttggc gggggctgga atctgcgttt gctggagaac ggcgaagagg
      241 tcggcggggg tgttttcccc ttgccggagc actgcgattt tcgggatgaa aaggcgctcc
      301 agacgctact cgatagcctc tatgaggacg ccttagcgga ggcctctgca tggctggcca
      361 gccgttaaag cgcctccgct ctatcaagat ccgggtctca gatgcggagc ttgagcgcct
      421 gcgggagctc tgccccaagg cccaactggc ggagtggatg cgggagcaat gtctcggtgt
      481 tgcgcaaccc cagcgccgaa cgccggcgcc taccgtagat ccggcgctct tgcggcagtt
      541 ggccggaatg ggcaacaacc tgaaccagat cgcccggcgc gttaattccg gggagtgggg
      601 gccgttggat cggctgcgca tcatagcgga gctgtccgct ataggccgcg agctggagga
      661 actgcaccat gatcatcaaa ttccataacc gtggggtagg ccggggctcc gggcctgttg
      721 gctacttgtt gggccgtgac gggaaccggg aaggcgcaac gctcgaccgg ggcaaccctg
      781 cggtgattga agccctgatt gattccagtc cctacgccaa aaaatacacc tctggcgttc
      841 tgtcgtttgc cgaggctgat ttacccaggg cgaccaagga caagctgatg agcgactttg
      901 aaaaggcgct cctgcccgga ctggagggca accagtacgc tgttttgtgg gtagagcacc
      961 gcgacaaggg gcggctggag ctgaattttg tagtcccgaa catcgaattg cagagcggga
     1021 agcggctgca accctacttc cacaaggccg acaatccgcg cctcgatgcg tggcgaaccg
     1081 tggtaaacgg ggagctgaaa ttgcacgacc cggacgatcc gttaaaccgt caggccgtta
     1141 tcacgccaaa ggatttgccg agggggcaca aggaggccgc tcaggccatt acagacgggc
     1201 ttttacgcat ggccggagca ggggagatcc agagccgcga tgatgtgcgc aaagccttgc
     1261 agggtgcggg tttcagcgtg gtacgcgaaa ccaaaaacag cctgagcatt gccgacccgg
     1321 acggggggcg gaacatccga ttaaaggggg tgctgtatga gcaagatttt agacatggcg
     1381 aaggacttcg agcagagctc gaagcagcag gccaacgcta tcgagaagcg tctgaccgaa
     1441 gaattcacga ggcacgagaa agctatctcc ggggcgttga aatcaagcga ggagaaaatg
     1501 agcgccgcta ttgccgagca cagccagaaa atgggctggc tgttgctcaa aacgtggatc
     1561 tggatgctgg tcagcatcgt cctgttcttg agcgcaacga gcgggatcct ctggtatcag
     1621 ggggaattga tagcggagcg gcaagccact ctccaagcct tggcagagaa gggcggcaag
     1681 ctaaagctga cccagtgcgg gaagaaattg gagcaccttt gcgtgcagat ctccgacccc
     1741 aatccaaccg tttgggggaa ggatacctat tggatcgtgc cgaaggggta ctaaatgacg
     1801 ggattggagc gggaattgct ctgcgcgttc gaggctttgc agagcgcata cgagcagcag
     1861 cacaaggcat ggcaggaaag ctacgcgaac ttggcgagca tgtacgagag caccaaacaa
     1921 gaggccgaga tcagcagggc agcagcagag cgattgacga ggcaggtgaa cggcttaacg
     1981 cagcaagtgg acggcttgag caagcagctg gagggcttga ccgggctagt cagcaagttg
     2041 caaaagaaag ggaacacagg ctcctgaagg cactggatcg aggctgggag cgtggagaca
     2101 gatcgcgagg ctttgagcgt tgacggaaca aaacggccgt cagttataaa aaaaggaaag
     2161 tgctatcact ttccttttat cggcgggaat tatcgttgag ttcttctatg ccatttttca
     2221 tagtagtaac tgtaatcttc atctctacca ccagattcaa tccagtcatc atactcaggg
     2281 ctatttttga aatctgaatc agacatgcta actccttgtt attagttggc ttctatggaa
     2341 gctagcgcac cgtgaagtta tgatcaacga tgtagtccac atcattgtcg aaatgtgcgt
     2401 tcagctagtc ttcggtgttt tctgctttat ttctagcggc gattctcgcg tctgcccatg
     2461 cggcagactt tttagcctgt tctcgttgtt cttcggttga agactcgctg ttgagaactt
     2521 tgttgctcgt ttgtttgacc accgaatcca aacctgttgt tgctgcaaaa tgagctaggt
     2581 taacaactcc cttacctaac aacttggcac tagcgattgc ggtttcttta gccccaaaag
     2641 catcagacac ctcctcaagc gctttagata ggttaaaaaa tcccatactc cccctaagaa
     2701 tatcatcaat aaaattgagt gttgctctgt ggataacttg cagagtttat taagtatcat
     2761 tgcagcaaag atgaaatcaa tgatttatca aaaatgattg aaaggtggtt gtaaataatg
     2821 ttacaatgtg tgagaagcag tctaaattct tcgtgaaata gtgatttttg aagctaataa
     2881 aaaacacacg tggaatttag gtaatagtta aagcttgtgc ctgattgttg ccacaattgg
     2941 tcagcagttt gctagggaat gagagggcag agaatgataa gcttagcact taatttgtat
     3001 ggatatattg cattaagcaa ggggacgtta tgcgtgtatt agccttatcg gctgtgtttt
     3061 tggtggcatc gattatcgga atgccagcgg tagcaaagga atggcaagaa aacaaaagtt
     3121 ggaatgctca ctttactgaa cataaatcac agggcgtagt tgtgctctgg aatgagaata
     3181 agcagcaagg atttaccaat aatcttaaac gggcgaacca agcattttta cccgcatcta
     3241 cctttaaaat tcccaatagc ttgatcgccc tcgatttggg cgtggttaag gatgaacacc
     3301 aagtctttaa gtgggatgga cagacgcgtg atatcgccgc ttggaatcgt gaccatgact
     3361 taattaccgc gatgaagtac tcagttgtgc ctgtttatca agaatttgcc cgccaaattg
     3421 gtgaggcacg tatgagtaaa atgctgcacg ccttcgatta tggcaatgag gatatctcgg
     3481 gcaatgtaga cagtttttgg ctcgatggtg gtattcgcat ttcggctacc cagcaaatcg
     3541 cttttttacg caagctgtat cacaacaagc tgcacgtttc tgagcgtagt cagcgcatcg
     3601 tgaaacaagc catgctgacc gaagccaatg gcgactatat tattcgggct aaaacgggat
     3661 actcgactag tatcgaacct aagattggct ggtgggttgg ttgggttgaa cttgatgata
     3721 atgtgtggtt ttttgcgatg aatatggata tgcccacatc ggatggttta gggctgcgcc
     3781 aagccatcac aaaagaagtg ctcaaacagg agaaaattat tccctagaag tggttagcgc
     3841 gtatttgtgt gaaatagccg tcatataagc tgtaaagata tatggacaaa atgctgatgc
     3901 tcgatgcgct tgtagttaat ggctcatact taatcggtca ggcctaatcg acttaagctc
     3961 cgcgcgttaa ctttcagcct gagcattagc atcacattcc taaacgtaaa aagcctagcc
     4021 aatttgctag gcttttttgt tatctcaatg gccatggtca ctcaacatgg gggtgtgaat
     4081 aaaggagtgg cgatgtcagt aaaggtgaat gccgctgcga taaagtgcct agcccatcaa
     4141 ggattgtagg ctttcttggc tgctaaagcg tttgacgaaa aagtccaaga aggcgctgat
     4201 attggttctc catcattttc atctaccggt ttactatagg tcaaccaggc cgtccttgcc
     4261 ggcgcttggg acgcagacga tggcatcaaa tgcctcgctc aaattcgttt ctacagaggc
     4321 gctttgggac cgcattcgct ttgctttcat gggggcatct gtcaatgtca gacatgaatc
     4381 ctccgatata ggttgatttt tgatctcttc aacacttggt gttgtccgac tagagagact
     4441 tatatggatc agtattcttg cactgacaat ggtgccactc aatcccaagg atcgacctag
     4501 ttagaggcgt agatcaagca attcaaaaca gcgcagaagc agaaggaggt ggaaatatgt
     4561 tccagggtga aaggttacat gcaactaacg ttgctctcta ggatgtgcct gctgtaacgt
     4621 attgtccaga aaagacaaac ccccgttatc gcttgcatct tggcggagct gcgaaaaacg
     4681 ggggccgtga cgaatcacaa gagaagtcta tcacatggat catgccaaac aaccccacgc
     4741 cgccaataag cgcggtggtg cttgagaaag tgcgcacagc ttgagctgtt tcgggagcgc
     4801 ctgccccgga aaccctacta ttccgacgag cttacaacgg gcctacggat tgcggatgtt
     4861 gcccgcgccc ttggtgcgcg ttacatccag ccgaacgggc caacgcaccg ccactggatc
     4921 gtttttgacg tagaccatgc agccgctacg ttatcttggg atgacgtagg cgctccagcc
     4981 cctaacatta ccgtcacgaa caaggccaac ggccacgcgc atctgatcta cggactggat
     5041 acgccgatcc gcactgcccc ggatggaaat gcagcccctc tgcggtatgc cgccgccatc
     5101 gaggccgcgc tccgggagaa attaggggcg gatatgggct actccgggct gatctgcaag
     5161 aaccccctgc atgagcactg gctggtgcag gtatgggaac cccggctcta cgatttagcc
     5221 tggctatccg attacctcga tctgagctcc tacaatggcc gcaaatcgct tcctgagtac
     5281 gggcttgggc gaaactgtac cctgttcgag aaaacgcgcc tgtgggccta taaagcgatc
     5341 cggcagggct ggccggatta ccccgaatgg ttggccgctt gtgttgaccg ggcatcagga
     5401 tataacgccc agttcgagca gccgctcccc gcaaacgaag tccggcacac ggccaagtcc
     5461 attgccaaat ggacgcacca gcacctaagc cccgcagggt tcagagagga gcaggcccgg
     5521 agaggtgcga agggcggcaa ggtatccaag ggagggggaa ggccgagcaa tagcggtaag
     5581 gacaagtccg atctattgcc ggaggttctg cggcttaagg ctcaggggta caccaaccgc
     5641 gatatagcgg acgatcttca aatctccccc agtacggtga gcgtttacct aaagcgtgat
     5701 cgtccgtgat cgaaacaagc cttatcagat aatagccccc tttagggggg ttcctgcgct
     5761 cttgtctttt agccttaaaa aatcctttta gcgcggatct ttggcgaggt ggacaactgc
     5821 gcctgtccgt ccatcgcccc cctgtttttt cggggggtgt gggcggcagc agttatccac
     5881 ggagccgctc tttcccttga tcttgctcta gggcttgcag tatcccgccc atggttgaat
     5941 aatgtactaa gcaaggctac acagaggccc ctctacctgc ttcgcggtct cgggcctctg
     6001 tggccttgtt caggggtgct acgccccccc gaaaccccct gtggcccccg ctcgatccgc
     6061 cccttcggga cggtctgcgc ggtggcttct tggctctgga gaatggacga ggccctatgt
     6121 caacaacaca gatagagaaa g
//
