LOCUS       NZ_CP012435            13136 bp    DNA     circular CON 20-APR-2024
DEFINITION  Enterococcus faecium strain ISMMS_VRE_1 plasmid ISMMS_VRE_p3,
            complete sequence.
ACCESSION   NZ_CP012435
VERSION     NZ_CP012435.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03988632
            Assembly: GCF_001720945.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13136)
  AUTHORS   Bashir,A., Attie,O., Sullivan,M., Sebra,R., Singh,K.V., Altman,D.,
            Pak,T., Dutta,J., Chacko,K., Webster,E., Lewis,M., Hamula,C., Delli
            Carpini,K.W., Murray,B.E., Kasarskis,A., van Bakel,H. and
            Huprikar,S.
  TITLE     Genomic confirmation of vancomycin-resistant Enterococcus
            transmission from deceased donor to liver transplant recipient
  JOURNAL   PLoS One 12 (3), e0170449 (2017)
   PUBMED   28301471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13136)
  AUTHORS   Bashir,A., Attie,O., Sebra,R., Singh,K.V., Altman,D., Pak,T.,
            Lewis,M., Dutta,J., Hamula,C., Delli Carpini,K.W., Murray,B.E.,
            Kasarskis,A., van Bakel,H. and Huprikar,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2015) Genetics and Genomic Sciences, Mount Sinai
            School of Medicine, 1425 Madison Avenue, New York, NY 10029, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2014
            Genome Coverage                   :: 218
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001720945.1-RS_2024_04_20
            Annotation Date                   :: 04/20/2024 01:22:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,263
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,001
            CDSs (with protein)               :: 3,001
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 79 of 168
            Pseudo Genes (incomplete)         :: 86 of 168
            Pseudo Genes (internal stop)      :: 33 of 168
            Pseudo Genes (multiple problems)  :: 27 of 168
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP012435.1.
            Bacteria available from Shirish Huprikar (shirish.
            huprikar@mssm.edu), Mount Sinai School of Medicine, One Gustave
            Levy Place,  New York, NY 10029.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="ISMMS_VRE_1"
                     /isolation_source="human blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="ISMMS_VRE_p3"
                     /geo_loc_name="USA: New York City"
                     /lat_lon="40.7127 N 74.0059 W"
                     /collection_date="2011-05-05"
                     /collected_by="Shirish Huprikar"
     gene            complement(join(13075..13136,1..404))
                     /locus_tag="AL014_RS16140"
                     /old_locus_tag="AL014_16160"
                     /pseudo=""
     CDS             complement(join(13075..13136,1..404))
                     /locus_tag="AL014_RS16140"
                     /old_locus_tag="AL014_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /name="DUF536 domain-containing protein"
     gene            complement(421..1353)
                     /locus_tag="AL014_RS16145"
                     /old_locus_tag="AL014_16165"
     CDS             complement(421..1353)
                     /locus_tag="AL014_RS16145"
                     /old_locus_tag="AL014_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002313300.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            1999..2085
                     /locus_tag="AL014_RS17765"
     CDS             1999..2085
                     /locus_tag="AL014_RS17765"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_111209149.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(2205..2894)
                     /locus_tag="AL014_RS16150"
                     /old_locus_tag="AL014_16170"
     CDS             complement(2205..2894)
                     /locus_tag="AL014_RS16150"
                     /old_locus_tag="AL014_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347611.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKVEEA
                     CSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(2896..3174)
                     /locus_tag="AL014_RS18455"
                     /old_locus_tag="AL014_16175"
     CDS             complement(2896..3174)
                     /locus_tag="AL014_RS18455"
                     /old_locus_tag="AL014_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            complement(3134..4048)
                     /locus_tag="AL014_RS16160"
                     /old_locus_tag="AL014_16180"
     CDS             complement(3134..4048)
                     /locus_tag="AL014_RS16160"
                     /old_locus_tag="AL014_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002305249.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4030..4410)
                     /gene="mobC"
                     /locus_tag="AL014_RS16165"
                     /old_locus_tag="AL014_16185"
     CDS             complement(4030..4410)
                     /gene="mobC"
                     /locus_tag="AL014_RS16165"
                     /old_locus_tag="AL014_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            complement(4852..4989)
                     /locus_tag="AL014_RS18020"
     CDS             complement(4852..4989)
                     /locus_tag="AL014_RS18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            complement(5103..5723)
                     /locus_tag="AL014_RS16170"
                     /old_locus_tag="AL014_16190"
     CDS             complement(5103..5723)
                     /locus_tag="AL014_RS16170"
                     /old_locus_tag="AL014_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(5720..7846)
                     /locus_tag="AL014_RS16175"
                     /old_locus_tag="AL014_16195"
     CDS             complement(5720..7846)
                     /locus_tag="AL014_RS16175"
                     /old_locus_tag="AL014_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323538.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            complement(7900..8274)
                     /locus_tag="AL014_RS16180"
                     /old_locus_tag="AL014_16200"
     CDS             complement(7900..8274)
                     /locus_tag="AL014_RS16180"
                     /old_locus_tag="AL014_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            8407..8868
                     /locus_tag="AL014_RS16185"
                     /old_locus_tag="AL014_16205"
     CDS             8407..8868
                     /locus_tag="AL014_RS16185"
                     /old_locus_tag="AL014_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            9405..9809
                     /gene="tnpA"
                     /locus_tag="AL014_RS16190"
                     /old_locus_tag="AL014_16210"
     CDS             9405..9809
                     /gene="tnpA"
                     /locus_tag="AL014_RS16190"
                     /old_locus_tag="AL014_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            9826..10974
                     /gene="tnpB"
                     /locus_tag="AL014_RS16195"
                     /old_locus_tag="AL014_16215"
     CDS             9826..10974
                     /gene="tnpB"
                     /locus_tag="AL014_RS16195"
                     /old_locus_tag="AL014_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(11203..12384)
                     /locus_tag="AL014_RS16200"
                     /old_locus_tag="AL014_16220"
     CDS             complement(11203..12384)
                     /locus_tag="AL014_RS16200"
                     /old_locus_tag="AL014_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /GO_process="GO:0051301 - cell division [Evidence IEA];
                     GO:0009252 - peptidoglycan biosynthetic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="WP_002302539.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKIT
                     KELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVLTG
                     AKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIATLPI
                     GILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISDTGRD
                     FLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNIHVPVR
                     ESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVAMIFFHV
                     FENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQK"
                     /name="FtsW/RodA/SpoVE family cell cycle protein"
     gene            complement(12674..12991)
                     /locus_tag="AL014_RS16205"
                     /old_locus_tag="AL014_16225"
     CDS             complement(12674..12991)
                     /locus_tag="AL014_RS16205"
                     /old_locus_tag="AL014_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_002302541.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKIMVKNYGKKSYKNTNTIISFCNFFKFSFHLNSVRILL
                     F"
                     /name="helix-turn-helix transcriptional regulator"
     BGC             2900..13136
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(422..1353)
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3138..4048)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aattcctctt cttcaggttc ttccgtataa gctaattgta actgttcttg cagtttttcg
       61 atctgttgat ttgcctgcaa ggtgagttgt tgttgctggt cgagtaactt ttgcatttgt
      121 tttatttgag tatctttttc ttggagctgt tcatgtaaaa aaacaataat cgagttttca
      181 tcgttactag caacggtttc tacttggtta ccagtggtgt tactacctaa aaaatgttgt
      241 tttaaaagca aatatccttt gtagctgaca actaaggttt tcactccgtt cctagtaaca
      301 gtttgcacat agttttctct aaaatcagct gatagccttt tcctaattgc ttgttttgaa
      361 acatgaaatt cttccgcgag ttctttgatc gttttatcta tcataaatac tccttatgct
      421 tcaatcgaat aagttttgta agttttctaa taaatcttgt ttaagctcat ttgagattga
      481 ttgtttttcc gcttcttctt tttcggtttg ttgttgtttt ttaaaatcag cttcatgagt
      541 acctaacgga agatttagta ttcgatcttt agcacgccat ttttcttttt gtgttaattc
      601 tccattgtgt tcaatattaa ataattttat acgtaaatct tcttgcttcc cttttgaaaa
      661 atcgtcagca tggttgattt cagctttcca agtaaaacga taaccaataa caggtttacc
      721 tcgaccttta ccatattttt tacgaatagt taatccttta aatagtggag ttagttcttc
      781 ttttattggt ttgagtacaa actgatttat atttgtttgt ctatagcttt taggaatatc
      841 taatagctct aaaaaatctt ccttagaaaa ttcagcccat ccttgagttc ggtattgttt
      901 tagcaatcta aacattgttt tagcataact gctatttaaa tctcgaaatt ctgttaaaga
      961 gaaacgtacc cagctttcta gattattaag taaaggcaaa gctttttcgt ggacttttat
     1021 atctacataa gggttatcga cttctccatg aatttcaaaa tcagtaaaca ttgtaaatcg
     1081 ttctattgtt aatccaccat tgctttttct tccgaaacgt aaatccatca aatgatcgta
     1141 tgttcttgcc aaatctttta taaagacttc tcctgcagtc tgcttatacg cacttaaatc
     1201 tttaagttgg ctgaaataaa agcgtatttt ttgattacct ttatcgcgca ttctagagac
     1261 aatagaaaaa aacaagttca tttctattgg ggtaaatttt cttaaaggaa tggtatttaa
     1321 ttctggttca tactttacga tttcatttgc catttagttg ttactccagt ctaaatgatt
     1381 ttattgacat cattttagga catattatgt ctaaaaacaa tacctaaaac agataatttc
     1441 atgcctataa acagataatt tcatgcctat aaacagataa tttcatgcct ataaacagat
     1501 aatttcatgc ctataaaatt ttgtatacct tgggggagta aggacacagc cgacgttaaa
     1561 gaggtttaaa aagaggttta aaaaaagatt ataaaagagg gagacaaaat ttcccaccta
     1621 aaaaaacaat tattttttct ttaaacaggt tcgcgaactt ttctaaactg ctaattcaaa
     1681 acctcgctcc ctacggtcgc caaaaaggtg tgatcgctac gctctcacat tatacctgcg
     1741 cagtcgcctc cagctccttt gctaaaacca ttgttcaaaa aggagggttg aggctctaag
     1801 agcttgtcta agccgtttta agacagtctt agtataaaaa actttatctg gaatttaaag
     1861 cgccctacag gcggtcaggg caagccctaa caacccggaa ataagagata cttaaaattg
     1921 ccacacagat ttaattttgt agggtataat aacttgtcgc ctatccgaca gtcttgcgac
     1981 tggaaggagg tgtatctgat gtttgaactg tttatttgcc cccttcttgt aggagtttgt
     2041 atctctgtat tcagttattg gttgaataac cgaaacaaga agtaaaatca gtaaaggcga
     2101 catcaaccta aaaaaagccc cagagaatcc gacctctctg gggctttcgt gtatctaatg
     2161 tttgaacatt tgcatgttta gtttaacatg aatttcaatc aaattcaatt ttgctgttgc
     2221 tgatctaacc attccttgac gctttttaaa taatttaaag ctgtttcttt tttagtcaga
     2281 ttttgtatgt taattgcttg ttgaagcaag atttcatagc gttcatatgt gactgattgt
     2341 tgtcgttctt ttcgttcttg ttcgctagtt gaaaaaggaa ttgtatctcc aaataaatca
     2401 ctacaagctt cttccacttt cgtatctttc acatattgca cgtctttttt ggttcgctca
     2461 tagttgtctt gaacctcttt ttgctgtttt tctggcaagt tttgataaag acgagtgagt
     2521 ttttccatgt ttttatccgt caaacgtttt tcttgattca caaaaatatc gttcatttcc
     2581 tcataacaag tggtaaactc ttccattttt tgtgctaatg cttgctcttt tttcttttta
     2641 tcacgcgctg attcatactt ttgagtgaca tttcctaata aaatacttct agaaacgcgc
     2701 ttatttccct ctaaaacgcc gaataagccg cttgaaacga ttatcaagga aaatatacag
     2761 ccaagaaaga aaaatcgctt aaatcgaatt ttagagcctt ttaatgaata tcttgtttga
     2821 aaaattttat agatgagcca aatacttcct gaaatactaa caaaaatgag tagatagggt
     2881 aaataaaatg ccattttagc ctccccatac taaccaaaat gcgaacagtg tcaggactaa
     2941 gcaacataag ggggcaagaa aatccaatga tcgttgaata agcacctttt tctgttcctt
     3001 atttcgctgg tctaatagct cttgatggag ttttttaact tgtaaatcaa tcgtagtttg
     3061 tatcctttct tgcgtataag gaacgagaaa agcacgttgt ttctttattt cgttttctat
     3121 gtcttttaac gttttagagc gataattctt gttggtggtc tgttcctctt tcaacgtgtt
     3181 gttcatggtt tcttcgagcc ggtctaattt ccggttgttc aggcgtaaga gttcgttgtt
     3241 ctcttcgtac ttcttctgca gtttcgcgaa tgctagctcg agctcttcgt agctcgttgg
     3301 ttcgctgttg gttataggtt cgttctcgtc tgtccagctc atgtaaaatc gtctcctttt
     3361 cgtagtcctt tcctagtctg ttggatcgta cttttttctt tttgtggatc aattcgtaag
     3421 tgaaattttt cccgcgattg aagcaatgaa tccccaatgt tttgagggaa ttacaaaatt
     3481 ggtcataggt tctggttttt ttgtttttca ttgtttgatc gatcgcaatc cgaatttcgt
     3541 ctttccaaac aggttcattt ttttctttta attttatttc agacatggtt cgtttttctg
     3601 gtttctttcc tcgctcagtg attggtaagt tatgtgcaag acaaatctcg tcatttaggt
     3661 cataacattt gtttagaaat gcttgaccat gagcttgaaa tttttttcca gtctccagat
     3721 taacggagtt aatgacaatg tgattatgaa tatggtcttt gtctgtatgt gtgtaaatag
     3781 ctatttggtg atcgggggct attttttttg catactctgc ccctagttga ttagcttttt
     3841 cagcggtaat ttctcctggt cgaaaagatt gaatcaataa gtgtgcctgt acacggtcgt
     3901 cttttccata cagcatgcga atttgtttca tttcactttt ggcataatcg acgtcacagt
     3961 ttattcctgt tttaatggtt gctcgtggtt ctgcgtaatt aattgcacga ctacaagaag
     4021 tggttcggct taccttaatt gttgccatat cttttgtagc tccttttgca tgatttttag
     4081 gttttcggat aaacgttgag cgtcaccaga agcaacgtca tgttggtgtg tattgcacca
     4141 cttagcaatt tgattgatat tccctccagc tttggctaaa tcgcgcgcga ttgcctgggc
     4201 aatgtctggc gccactttcg gtgtcaccag acgcgcgttt tgtgccttgg ctttcacgaa
     4261 agccggcaca ctcatttgca aagcttcagc cgattgcttc agctttgcaa attcggattc
     4321 gctcactcga aaactgattt gttttggctc ggatcgatag gtatattttt tagaagaatt
     4381 gctagccaaa ttttgatttt gttcgctcat aagtcccacc ctttcagtac gtcctacgga
     4441 cgtatcgttc gcaataccat tgtacctaaa aaggtacaat gagtaaagct cactaaaagg
     4501 gtttggcaag ctctatattt gctagccaaa tacctacgta tttagtgcaa ataaccgctt
     4561 gtcaaagggg cgcaagctcc cccttgaatc cccctcaaaa gccacgctcc acaatacaaa
     4621 aaaagctagc aaatgctagc cactttttgt aaagtttcgc ttatttcacc gaagatgaaa
     4681 ttgttgcttt gcaacaaaaa acataaatgc ttttttgtgt aaactactaa ctatttagct
     4741 aaaactgata gttttattaa acattcagca atatttttag ccaatttttt atcagacttt
     4801 cggcgagtca ttatgataat atgtccactt tttgttactt ctaccaaaaa tctagcaata
     4861 actctcccat tcttcagttg atttagtttc ataagtcttt tgtaattgac ggtttaattt
     4921 ttttggcaat tgcaaagaat aaataagtct atcataaaat ttttcatact cttttttcaa
     4981 taaaatcact tgggtgcctc ctttttatta agtgattatt taaattttgg gataggagag
     5041 atggaatata aaataaatac ccgtggtgct agttttaaat cagctagcat cacgggtaaa
     5101 aattataatt ctataatatt agttccatag tttagaacat ttaagtcatg tgttactagt
     5161 acaacggctt ttttttcttt ttctgttaaa tttttcagaa agttcattat aaaagttgag
     5221 ttttctttat ctaaatttcc tgtaggttca tctgcaaata atatatcaaa tggttttaac
     5281 atatttctaa ttaatgctat tctttgttgt tctccaccgc ttaagagaaa gattttttct
     5341 tttaagatat cttttggcaa agaaaaatct gttaataaat tataaatatg atcattggaa
     5401 atattactgt taaacttttt tgctaattcg aaattgtatt gtactgtttg attttcaaga
     5461 agagcatagt tttgaaataa atagctaata tgatttcttc gtagacgttg tatatcatgt
     5521 ttagaaactt tcttttcaaa atagaataag ttaccggtat attgtttatc tattaggctt
     5581 aaattattta acagagtagt ttttccacta ccactttttc ctgttactat aataaaatct
     5641 ttttctgata ttgaaaagtt ccaattttgt ttgatctttt ttttattaat tgacagggtg
     5701 aaattctcaa ctgataatat cataaaatat tctccttaaa atatttaaaa ctattttttt
     5761 tataaataat aatagaagta attagattta ttaaaaagat cataaaaatt ataaaataca
     5821 agtaagtgtt ttttgaaaat atcaattgaa aacacatagt agcaaataac tctattgtaa
     5881 aagggagtaa acataaatct aaaacttgac ttattggtga atatccaaat atttttttta
     5941 aagctatttt ttttgaattt atagtaaaac taacaaaaat catttgaaaa ttaattatta
     6001 taaataatcc taatattaaa agatattgta gatatgcatt taatttattt actttttttt
     6061 gagataataa ttcaccagtt tcttgaaaaa cagaataaga aaaatttatg atattttcta
     6121 ggttaaattc ttttatatac ggattaattt gattaaaaaa gttttggttt gaagtatgtt
     6181 tctgatcaaa aaataatcca ccatcactag tcaatatatt tccccaaaat aatgattctg
     6241 ttatttctgg attaataact ctaactatgg gatcagttat tgtatagtta tcttttgcct
     6301 ttccatataa ggtattaaaa ctaaaatact tttggttatt attaacaaat attagtttta
     6361 tttttaaatt tttcaaatta ggatttttat aaggagcttg aggaggagag tcctcaagtc
     6421 taaactgtat gtcttctaaa taattttttt caatcatttt tgcatattct ttatattttt
     6481 taggtactag aatgtaagta gttagtggat cagagtttat taaatttaag tcagtagatt
     6541 cttcaaaaga aataggattg taatatttta gatagtttaa atcaacacat attcctgcac
     6601 cagcaggttg actaacatag tcttcaaaat ttggataaac tttttgtcca ataaaaaatt
     6661 ctttattatt ctcttggata gttgcgtaat tgtatggcgc gataataaaa gttttataat
     6721 tcttctgtat tttataaaca aatttactag cttttttcaa gtagttattt tcttctacgg
     6781 tgttgtttct atttaattga ttagtaatat ttgttttgta taaattagta gttctttccc
     6841 aatatttaag agaattattt tcggcttcat tatgggtatt tattactttt gtagaagcta
     6901 ttaacagaag gatcatgctt aagaatagac agatatttaa tatacaaaga tttaacttaa
     6961 gtggtcgtga attttttaga taatttttta tactagataa atgatagata cttagagtaa
     7021 ctatgataaa taaaaaacta attataaaat gaaaaatact acatagaaga aatgatagga
     7081 aaaaattttc gttactgtaa tatatgctca aaaaaataga taaaatgaaa gttataatca
     7141 aagaacaaaa taagaggttt aactctttaa tagaatcttt tatagtatca aaagagttat
     7201 agcctaacat tttttttaga gtgatttctt tcatttttga aaaagcaaat ataattgtag
     7261 taattacaat aaatatacta agatagcaga taacaagaac aatattagta ttaatttgaa
     7321 aagtatctga ttgattgata ttggaaatag taggtgttcc atattttttc agtacaggaa
     7381 ttaatttgtt tgagtttcct tgtacatata atatttcact taatccaata ttttccacat
     7441 gagaaaaagg ataaatattt atatttaaaa aattatttgg aagttttaat tggtacttag
     7501 aatttttgtt attagtataa tggactttag aatcgtttct tgctaatata ttttttttag
     7561 aaaatttcca gtgtttgtta gcgttagggt ttgtagcaag aatagttagt tggttaacag
     7621 cagtaaaact atattgagaa atattaatat tttcttgtgt agatatttca tacaaagcgt
     7681 ttatataact gttaaagtcc atatcatctt tatattgcat atgaatttca gcggtatttt
     7741 tatatagcgc ttcagaagtc tttataccat ttataacatt agtagaaaaa aatagtattg
     7801 taatgcagac aaaaagtgat aaaacagcgt ttattttttt taacatattt tgaatttcct
     7861 ttcaaatgta aataaaaaag agagtttaaa ctctcttttt taataaacat tccaccaacc
     7921 agtatttcca gtaagtgtgg cattgcatgt tgcataagct cttactccag ctttctgaac
     7981 tgatcgtact tgaccgcctc gtccattttt agctgttgca ctatgtttag ctctagcatg
     8041 gtagtaattt gcataaactt taaatccatt aaccccacag ttgaaatccc cgccgccagc
     8101 agcatatctc aatttactag aagaaaatcc ttcttctgta gcttccattc ctcctccgta
     8161 aatggcggta tggtcttcgg gtacatctag atttaaagct tctgtggcac tagctattcc
     8221 tccactagtt aataagacac ccattaaagc agaaagacat aaaaattttt tcataactta
     8281 tttctcctat cttttttact agaattattt tattttagtg tgttttataa aataaagaac
     8341 ttttttggcg cgttagtatt tttatgataa aattttatgt aaataatcgt ttacatagga
     8401 gaaaaaatgg aggatttagg atctaagcta agagaattaa gagtgaaaaa agggtatact
     8461 caagaatatg tggcaagaaa atttaacgtt acacgtcaaa ctatttcaaa gtgggaaaat
     8521 aacaaaacaa caccagatat agagacacta aaaaaaattt gtgaatttta tagtactaac
     8581 ataaattcta ttttaaatga agataattca aaaaataaaa aaaataattt actggtattg
     8641 acagctatta tttttaatat atttgttact atattaggtt tatttgaatt gattcaaatg
     8701 aattttttta gctttacaca tggaatattt acttggtttg ctctatctgg attaatagta
     8761 atagatatct taaattattt ttatatttac tattcttatt taaaaaatga aaaatttttt
     8821 tatacaatgt ttttccccct agttattttg attattttat taagctaata tgaagtagtt
     8881 agttatttaa ttgcatagtt gacataatgt cgactatgcc aagtagcctc tctaccaagg
     8941 gatacagggg ctcactttgt gatttttcat tggtctaggt caaaagctct ttttaaggct
     9001 tgattttctg cagttgcttc tttccgtttc tttgtgaatt tttaccagtc attttttact
     9061 ggggtgttat ttttcataaa atatgataaa tttttggtag cgcttcataa gcgcgttgct
     9121 ttttcaagaa taaacactca ttatttttta actggctgct cgaaagagca gtctttttta
     9181 ttatttatcc gtttgtttta caaacaattc ttcgttagga ctgttgtaat aatttgaatt
     9241 taacagaatt aggcagaagt cgcccatcct ctataggtgg gtgatgaatg ccgttcggta
     9301 tgagggttac cgttggtaac tcgaagaccg acaactaagg cgtattgcct ttttattttg
     9361 ttccatgtta caatcagtct atacaagaaa tgagttgata taacatggaa ttagatacaa
     9421 ataatcattc agtatttctt ctttattacc accttgtttt agtaacgaaa tatcgtagac
     9481 aagtgattga tgatgaaata tctgattatg ccaaaactac ttttgaaaga atttcagaat
     9541 cgtatcacat tactttagtg gagtggaatc atgataaaga ccacgttcat attatgttca
     9601 aagctcaacc taaaacagaa ttgacaaaat tcatcaatgc ctataaaagt gccagctcaa
     9661 gattgataaa acgtgacttt ccaagagtca aacaatttct ttggaaagaa atgttctggt
     9721 ctaaaagttt ttgtcttctg acaactggtg gcgcaccaat tgatgtgatt aagaagtata
     9781 tccaaaatca aggaaacaat cataaataga aagtaggtga actttatgga acaactaaaa
     9841 gcatacaaat tcagaatcta tccaacagag gaacaagaaa tcttctttgc caaatctttt
     9901 ggttgtgtcc gtaaggtcta caatctaatg cttgatgacc gaaaaaaggc gtatgaagaa
     9961 gttaaaaatg attcttctaa aaaaatgact tttccgacac cagctaaata caagaaagaa
    10021 tttccgtttt tgaaagaaat tgatagcctt gctttagcca atgcccaact taatttagat
    10081 aaagcataca agaatttttt tcgggataaa tccgttggat ttccacgttt caaaagtaag
    10141 aaaaatcctg ttcagagtta tacaacgaat aatcaaaatg gtacagttgc tttgattgat
    10201 agtaaattca tcaaagttcc taaattgaag tcattagtaa gaattaagct tcatagacaa
    10261 ccaaaaggga ttatcaaatc cgctacaata tcacgtcatt ctagcggtaa atactatatt
    10321 tctttgttgt gtaaagaaga aattatcgaa ttacctaaaa ctaattccac aattggtatt
    10381 gacttaggta ttatggactt tgcgattctt tctgatggac aaaaaattga taataataaa
    10441 ttcacgtcca aaatggaaaa gaaactaaag cgtgaacaac gcaaattgtc taggcgtgct
    10501 ttattggcta aaaataaagg taccaatcta tttgaagcta aaaattatca aaaacaaaaa
    10561 cgcaaagtag ctagattgca tgaaaaagta atgaatcaac gtactgattt tctgaataag
    10621 ttgagtacag aaatgatcaa aaatcacgat attatctgta ttgaagactt aaacacaaaa
    10681 ggtatgttgc gaaatcataa attagctaaa agtatttctg atgtttcttg gtcaaagttt
    10741 gtgactaaat tacaatacaa ggctgactgg tatggacgaa aaatcatcaa agtagataaa
    10801 tggtttccgt ctagtcagat ttgttcagag tgcggacata aagatggcaa gaaatctctt
    10861 gaaattcgag aatggacttg tcctgtttgt catactcatc atgatcgtga tatcaatgct
    10921 agtatcaata ttctgaccga aggtctaaga ctacattcta tgggattggc ttagatccaa
    10981 tcatcaagaa ccgtaggaac tacggggata gcttggtaaa taagagacac cgctgttagt
    11041 aaagaaatac gctatcaagt atgctctatt cccaagaagc tcccacttca agcgttaaga
    11101 accgtcaggt ttagctaagt ggtgagtagt tcactgatta actttaaggt atcataattt
    11161 ttattgaaat agacattcta ttcataaagt gttattttga attcattttt gtgttccttt
    11221 tttttcgtta gtatatctac tacaacacaa cataaatccc aaccccaaaa aattactaag
    11281 aatggcagaa ccaccttgac taatataggg gagtggaata ccagttagag gtagtaatcc
    11341 gatacttgca cctatatttt caaaaacatg aaaaaaaatc attgcaacaa tgcctatacc
    11401 gatataggtg aaaaataagt tattgctttt aaaacatgtt ttatacattt gataaattaa
    11461 ataaaagtat aggaatatta caagacaact tcctataaag ccaaaatttt ctccaattac
    11521 agaaaatatc atatcggatt ctctaacagg aacgtgaatg tttgatacat taaaaccagt
    11581 acctaaaagt ccgccagagc caatagcaat taaactattt gctaactgat agctagatcc
    11641 atttggatct aaaaatggat tcaaccatga gtctattctt ttaaactgat agtcgtgaaa
    11701 tcctattttt aataagaaat ctcttcctgt atctgatata accaaatata aaaatattaa
    11761 ggataaaaca acaccaatgg cgattattgg ggcaattatt tttatattta tgttagacgt
    11821 aaacaaaata aaaatataaa taacaatgaa gactaacatt gttcctagat cgttctgaaa
    11881 aaataataat attcctattg gaagagtagc aataaaagtt ttaagtaaat aaatccaatc
    11941 atctttcgta ttactagtaa tagatttttg ctgataattg acggttattt tagctagcat
    12001 aagggtgtat cctattttga ctaactcagt aggttgaaac gaaaaaccag caatataaaa
    12061 ccaatttttt gctccagtta agactgctaa ttgtggatta tagaatttca agagaagtaa
    12121 taacattatg atgcataatc cgtaaaatat aggagttaat ttccacaaaa ttttaggatt
    12181 aaaaaaataa atagttattg ctgaagtgaa cccaatacta taccacaata cttgtttaat
    12241 taattctttt gtgatttttg aattagtgta agagtcgtga tataaagcaa catataaaga
    12301 aagtaatcca atgatacata gcagaaaaac tggcaataaa agcccataat tcaactgatt
    12361 tttgtagatt ctattatttt tcatttcgaa aggtcctttt tcataattta taaataaaaa
    12421 taacacaata aagttatttt tactagtttt gagttttaga cgaaaattac aattagctac
    12481 ttttttatct tactaaatag ttctatcttt ttatataaag tatagaaaaa ttaataaaac
    12541 aattggaaat atgctgtgaa gaaataagtt tctatgttta ttaaagataa taattaaagt
    12601 attaattatt aatgtattaa ttattaatag taaacttaga aaaaatatta ataaccaagt
    12661 ggcaattgaa tggctaaaat aaaagaatcc ggactgaatt aaggtgaaaa ctaaacttaa
    12721 aaaaattaca aaatgaaata atagtattag tgtttttgta tgatttttta ccataatttt
    12781 ttaccataat tttttcttct cctaacaaaa tattgtctag tgataaatca aataacttag
    12841 caaactgaat taaattttct gtatctggta ttgttcgacc attttcccaa ttagaaatgg
    12901 tttttttaga tactaaaagc atttcagcta aatcttcttg agttagttgt ttttctattc
    12961 ttttttgttt gattacttta cttatagtca tttgatcacc taaacatatt ttttattctt
    13021 agaaagatag agtctataga aattgatttc ttctttatgg aaagttggta attgttacca
    13081 aaatttccac cacttttttt ctgttgttat tgcattgttg ttttctttat tgacag
//
