LOCUS       NZ_CP012434            13123 bp    DNA     circular CON 20-APR-2024
DEFINITION  Enterococcus faecium strain ISMMS_VRE_1 plasmid ISMMS_VRE_p4,
            complete sequence.
ACCESSION   NZ_CP012434
VERSION     NZ_CP012434.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03988632
            Assembly: GCF_001720945.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13123)
  AUTHORS   Bashir,A., Attie,O., Sullivan,M., Sebra,R., Singh,K.V., Altman,D.,
            Pak,T., Dutta,J., Chacko,K., Webster,E., Lewis,M., Hamula,C., Delli
            Carpini,K.W., Murray,B.E., Kasarskis,A., van Bakel,H. and
            Huprikar,S.
  TITLE     Genomic confirmation of vancomycin-resistant Enterococcus
            transmission from deceased donor to liver transplant recipient
  JOURNAL   PLoS One 12 (3), e0170449 (2017)
   PUBMED   28301471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13123)
  AUTHORS   Bashir,A., Attie,O., Sebra,R., Singh,K.V., Altman,D., Pak,T.,
            Lewis,M., Dutta,J., Hamula,C., Delli Carpini,K.W., Murray,B.E.,
            Kasarskis,A., van Bakel,H. and Huprikar,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2015) Genetics and Genomic Sciences, Mount Sinai
            School of Medicine, 1425 Madison Avenue, New York, NY 10029, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2014
            Genome Coverage                   :: 218
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001720945.1-RS_2024_04_20
            Annotation Date                   :: 04/20/2024 01:22:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,263
            CDSs (total)                      :: 3,169
            Genes (coding)                    :: 3,001
            CDSs (with protein)               :: 3,001
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 79 of 168
            Pseudo Genes (incomplete)         :: 86 of 168
            Pseudo Genes (internal stop)      :: 33 of 168
            Pseudo Genes (multiple problems)  :: 27 of 168
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP012434.1.
            Bacteria available from Shirish Huprikar (shirish.
            huprikar@mssm.edu), Mount Sinai School of Medicine, One Gustave
            Levy Place,  New York, NY 10029.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="ISMMS_VRE_1"
                     /isolation_source="human blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="ISMMS_VRE_p4"
                     /geo_loc_name="USA: New York City"
                     /lat_lon="40.7127 N 74.0059 W"
                     /collection_date="2011-05-05"
                     /collected_by="Shirish Huprikar"
     gene            complement(join(13112..13123,1..2010))
                     /locus_tag="AL014_RS18045"
                     /old_locus_tag="AL014_16090"
     CDS             complement(join(13112..13123,1..2010))
                     /locus_tag="AL014_RS18045"
                     /old_locus_tag="AL014_16090"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227005129.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFCIL"
                     /name="hypothetical protein"
     gene            complement(2064..2438)
                     /locus_tag="AL014_RS16075"
                     /old_locus_tag="AL014_16095"
     CDS             complement(2064..2438)
                     /locus_tag="AL014_RS16075"
                     /old_locus_tag="AL014_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            2571..3035
                     /locus_tag="AL014_RS16080"
                     /old_locus_tag="AL014_16100"
     CDS             2571..3035
                     /locus_tag="AL014_RS16080"
                     /old_locus_tag="AL014_16100"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_227005130.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFIQCFSPSYFDYFIKLI"
                     /name="helix-turn-helix domain-containing protein"
     gene            3567..3971
                     /gene="tnpA"
                     /locus_tag="AL014_RS16085"
                     /old_locus_tag="AL014_16105"
     CDS             3567..3971
                     /gene="tnpA"
                     /locus_tag="AL014_RS16085"
                     /old_locus_tag="AL014_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            3988..5136
                     /gene="tnpB"
                     /locus_tag="AL014_RS16090"
                     /old_locus_tag="AL014_16110"
     CDS             3988..5136
                     /gene="tnpB"
                     /locus_tag="AL014_RS16090"
                     /old_locus_tag="AL014_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(5365..6546)
                     /locus_tag="AL014_RS16095"
                     /old_locus_tag="AL014_16115"
     CDS             complement(5365..6546)
                     /locus_tag="AL014_RS16095"
                     /old_locus_tag="AL014_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /GO_process="GO:0051301 - cell division [Evidence IEA];
                     GO:0009252 - peptidoglycan biosynthetic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="WP_002302539.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKIT
                     KELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVLTG
                     AKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIATLPI
                     GILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISDTGRD
                     FLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNIHVPVR
                     ESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVAMIFFHV
                     FENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQK"
                     /name="FtsW/RodA/SpoVE family cell cycle protein"
     gene            complement(6836..7153)
                     /locus_tag="AL014_RS16100"
                     /old_locus_tag="AL014_16120"
     CDS             complement(6836..7153)
                     /locus_tag="AL014_RS16100"
                     /old_locus_tag="AL014_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_002302541.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKIMVKNYGKKSYKNTNTIISFCNFFKFSFHLNSVRILL
                     F"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(7237..7707)
                     /locus_tag="AL014_RS16105"
                     /old_locus_tag="AL014_16125"
     CDS             complement(7237..7707)
                     /locus_tag="AL014_RS16105"
                     /old_locus_tag="AL014_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002302544.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTLV
                     VSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            complement(7724..8656)
                     /locus_tag="AL014_RS16110"
                     /old_locus_tag="AL014_16130"
     CDS             complement(7724..8656)
                     /locus_tag="AL014_RS16110"
                     /old_locus_tag="AL014_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002313300.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            9302..9388
                     /locus_tag="AL014_RS17760"
     CDS             9302..9388
                     /locus_tag="AL014_RS17760"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_111209149.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(9508..10197)
                     /locus_tag="AL014_RS16115"
                     /old_locus_tag="AL014_16135"
     CDS             complement(9508..10197)
                     /locus_tag="AL014_RS16115"
                     /old_locus_tag="AL014_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347611.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKVEEA
                     CSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(10199..10477)
                     /locus_tag="AL014_RS18450"
                     /old_locus_tag="AL014_16140"
     CDS             complement(10199..10477)
                     /locus_tag="AL014_RS18450"
                     /old_locus_tag="AL014_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            complement(10437..11351)
                     /locus_tag="AL014_RS16125"
                     /old_locus_tag="AL014_16145"
     CDS             complement(10437..11351)
                     /locus_tag="AL014_RS16125"
                     /old_locus_tag="AL014_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002305249.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(11333..11713)
                     /gene="mobC"
                     /locus_tag="AL014_RS16130"
                     /old_locus_tag="AL014_16150"
     CDS             complement(11333..11713)
                     /gene="mobC"
                     /locus_tag="AL014_RS16130"
                     /old_locus_tag="AL014_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            complement(12155..12292)
                     /locus_tag="AL014_RS18015"
     CDS             complement(12155..12292)
                     /locus_tag="AL014_RS18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            complement(12406..13026)
                     /locus_tag="AL014_RS16135"
                     /old_locus_tag="AL014_16155"
     CDS             complement(12406..13026)
                     /locus_tag="AL014_RS16135"
                     /old_locus_tag="AL014_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     BGC             1..7438
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(7725..8656)
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(10441..11351)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaatatcaat tgaaaacaca tagtagcaaa taactctatt gtaaaaggga gtaaacataa
       61 atctaaaact tgacttattg gtgaatatcc aaatattttt tttaaagcta ttttttttga
      121 atttatagta aaactaacaa aaatcatttg aaaattaatt attataaata atcctaatat
      181 taaaagatat tgtagatatg catttaattt atttactttt ttttgagata ataattcacc
      241 agtttcttga aaaacagaat aagaaaaatt tatgatattt tctaggttaa attcttttat
      301 atacggatta atttgattaa aaaagttttg gtttgaagta tgtttctgat caaaaaataa
      361 tccaccatca ctagtcaata tatttcccca aaataatgat tctgttattt ctggattaat
      421 aactctaact atgggatcag ttattgtata gttatctttt gcctttccat ataaggtatt
      481 aaaactaaaa tacttttggt tattattaac aaatattagt tttattttta aatttttcaa
      541 attaggattt ttataaggag cttgaggagg agagtcctca agtctaaact gtatgtcttc
      601 taaataattt ttttcaatca tttttgcata ttctttatat tttttaggta ctagaatgta
      661 agtagttagt ggatcagagt ttattaaatt taagtcagta gattcttcaa aagaaatagg
      721 attgtaatat tttagatagt ttaaatcaac acatattcct gcaccagcag gttgactaac
      781 atagtcttca aaatttggat aaactttttg tccaataaaa aattctttat tattctcttg
      841 gatagttgcg taattgtatg gcgcgataat aaaagtttta taattcttct gtattttata
      901 aacaaattta ctagcttttt tcaagtagtt attttcttct acggtgttgt ttctatttaa
      961 ttgattagta atatttgttt tgtataaatt agtagttctt tcccaatatt taagagaatt
     1021 attttcggct tcattatggg tatttattac ttttgtagaa gctattaaca gaaggatcat
     1081 gcttaagaat agacagatat ttaatataca aagatttaac ttaagtggtc gtgaattttt
     1141 tagataattt tttatactag ataaatgata gatacttaga gtaactatga taaataaaaa
     1201 actaattata aaatgaaaaa tactacatag aagaaatgat aggaaaaaat tttcgttact
     1261 gtaatatatg ctcaaaaaaa tagataaaat gaaagttata atcaaagaac aaaataagag
     1321 gtttaactct ttaatagaat cttttatagt atcaaaagag ttatagccta acattttttt
     1381 tagagtgatt tctttcattt ttgaaaaagc aaatataatt gtagtaatta caataaatat
     1441 actaagatag cagataacaa gaacaatatt agtattaatt tgaaaagtat ctgattgatt
     1501 gatattggaa atagtaggtg ttccatattt tttcagtaca ggaattaatt tgtttgagtt
     1561 tccttgtaca tataatattt cacttaatcc aatattttcc acatgagaaa aaggataaat
     1621 atttatattt aaaaaattat ttggaagttt taattggtac ttagaatttt tgttattagt
     1681 ataatggact ttagaatcgt ttcttgctaa tatatttttt ttagaaaatt tccagtgttt
     1741 gttagcgtta gggtttgtag caagaatagt tagttggtta acagcagtaa aactatattg
     1801 agaaatatta atattttctt gtgtagatat ttcatacaaa gcgtttatat aactgttaaa
     1861 gtccatatca tctttatatt gcatatgaat ttcagcggta tttttatata gcgcttcaga
     1921 agtctttata ccatttataa cattagtaga aaaaaatagt attgtaatgc agacaaaaag
     1981 tgataaaaca gcgtttattt tttttaacat attttgaatt tcctttcaaa tgtaaataaa
     2041 aaagagagtt taaactctct tttttaataa acattccacc aaccagtatt tccagtaagt
     2101 gtggcattgc atgttgcata agctcttact ccagctttct gaactgatcg tacttgaccg
     2161 cctcgtccat ttttagctgt tgcactatgt ttagctctag catggtagta atttgcataa
     2221 actttaaatc cattaacccc acagttgaaa tccccgccgc cagcagcata tctcaattta
     2281 ctagaagaaa atccttcttc tgtagcttcc attcctcctc cgtaaatggc ggtatggtct
     2341 tcgggtacat ctagatttaa agcttctgtg gcactagcta ttcctccact agttaataag
     2401 acacccatta aagcagaaag acataaaaat tttttcataa cttatttctc ctatcttttt
     2461 tactagaatt attttatttt agtgtgtttt ataaaataaa gaactttttt ggcgcgttag
     2521 tatttttatg ataaaatttt atgtaaataa tcgtttacat aggagaaaaa atggaggatt
     2581 taggatctaa gctaagagaa ttaagagtga aaaaagggta tactcaagaa tatgtggcaa
     2641 gaaaatttaa cgttacacgt caaactattt caaagtggga aaataacaaa acaacaccag
     2701 atatagagac actaaaaaaa atttgtgaat tttatagtac taacataaat tctattttaa
     2761 atgaagataa ttcaaaaaat aaaaaaaata atttactggt attgacagct attattttta
     2821 atatatttgt tactatatta ggtttatttg aattgattca aatgaatttt tttagcttta
     2881 cacatggaat atttacttgg tttgctctat ctggattaat agtaatagat atcttaaatt
     2941 atttttatat ttactattct tatttaaaaa atgaaaaatt ttttatacaa tgtttttccc
     3001 ctagttattt tgattatttt attaagctaa tatgaagtag ttagttattt aattgcatag
     3061 ttgacataat gtcgactatg ccaagtagcc tctctaccaa gggatacagg ggctcacttt
     3121 gtgatttttc attggtctag gtcaaaagct ctttttaagg cttgattttc tgcagttgct
     3181 tctttccgtt tctttgtgaa tttttaccag tcatttttta ctggggtgtt atttttcata
     3241 aaatatgata aatttttggt agcgcttcat aagcgcgttg ctttttcaag aataaacact
     3301 cattattttt taactggctg ctcgaaagag cagtcttttt tattatttat ccgtttgttt
     3361 tacaaacaat tcttcgttag gactgttgta ataatttgaa tttaacagaa ttaggcagaa
     3421 gtcgcccatc ctctataggt gggtgatgaa tgccgttcgg tatgagggtt accgttggta
     3481 actcgaagac cgacaactaa ggcgtattgc ctttttattt tgttccatgt tacaatcagt
     3541 ctatacaaga aatgagttga tataacatgg aattagatac aaataatcat tcagtatttc
     3601 ttctttatta ccaccttgtt ttagtaacga aatatcgtag acaagtgatt gatgatgaaa
     3661 tatctgatta tgccaaaact acttttgaaa gaatttcaga atcgtatcac attactttag
     3721 tggagtggaa tcatgataaa gaccacgttc atattatgtt caaagctcaa cctaaaacag
     3781 aattgacaaa attcatcaat gcctataaaa gtgccagctc aagattgata aaacgtgact
     3841 ttccaagagt caaacaattt ctttggaaag aaatgttctg gtctaaaagt ttttgtcttc
     3901 tgacaactgg tggcgcacca attgatgtga ttaagaagta tatccaaaat caaggaaaca
     3961 atcataaata gaaagtaggt gaactttatg gaacaactaa aagcatacaa attcagaatc
     4021 tatccaacag aggaacaaga aatcttcttt gccaaatctt ttggttgtgt ccgtaaggtc
     4081 tacaatctaa tgcttgatga ccgaaaaaag gcgtatgaag aagttaaaaa tgattcttct
     4141 aaaaaaatga cttttccgac accagctaaa tacaagaaag aatttccgtt tttgaaagaa
     4201 attgatagcc ttgctttagc caatgcccaa cttaatttag ataaagcata caagaatttt
     4261 tttcgggata aatccgttgg atttccacgt ttcaaaagta agaaaaatcc tgttcagagt
     4321 tatacaacga ataatcaaaa tggtacagtt gctttgattg atagtaaatt catcaaagtt
     4381 cctaaattga agtcattagt aagaattaag cttcatagac aaccaaaagg gattatcaaa
     4441 tccgctacaa tatcacgtca ttctagcggt aaatactata tttctttgtt gtgtaaagaa
     4501 gaaattatcg aattacctaa aactaattcc acaattggta ttgacttagg tattatggac
     4561 tttgcgattc tttctgatgg acaaaaaatt gataataata aattcacgtc caaaatggaa
     4621 aagaaactaa agcgtgaaca acgcaaattg tctaggcgtg ctttattggc taaaaataaa
     4681 ggtaccaatc tatttgaagc taaaaattat caaaaacaaa aacgcaaagt agctagattg
     4741 catgaaaaag taatgaatca acgtactgat tttctgaata agttgagtac agaaatgatc
     4801 aaaaatcacg atattatctg tattgaagac ttaaacacaa aaggtatgtt gcgaaatcat
     4861 aaattagcta aaagtatttc tgatgtttct tggtcaaagt ttgtgactaa attacaatac
     4921 aaggctgact ggtatggacg aaaaatcatc aaagtagata aatggtttcc gtctagtcag
     4981 atttgttcag agtgcggaca taaagatggc aagaaatctc ttgaaattcg agaatggact
     5041 tgtcctgttt gtcatactca tcatgatcgt gatatcaatg ctagtatcaa tattctgacc
     5101 gaaggtctaa gactacattc tatgggattg gcttagatcc aatcatcaag aaccgtagga
     5161 actacgggga tagcttggta aataagagac accgctgtta gtaaagaaat acgctatcaa
     5221 gtatgctcta ttcccaagaa gctcccactt caagcgttaa gaaccgtcag gtttagctaa
     5281 gtggtgagta gttcactgat taactttaag gtatcataat ttttattgaa atagacattc
     5341 tattcataaa gtgttatttt gaattcattt ttgtgttcct tttttttcgt tagtatatct
     5401 actacaacac aacataaatc ccaaccccaa aaaattacta agaatggcag aaccaccttg
     5461 actaatatag gggagtggaa taccagttag aggtagtaat ccgatacttg cacctatatt
     5521 ttcaaaaaca tgaaaaaaaa tcattgcaac aatgcctata ccgatatagg tgaaaaataa
     5581 gttattgctt ttaaaacatg ttttatacat ttgataaatt aaataaaagt ataggaatat
     5641 tacaagacaa cttcctataa agccaaaatt ttctccaatt acagaaaata tcatatcgga
     5701 ttctctaaca ggaacgtgaa tgtttgatac attaaaacca gtacctaaaa gtccgccaga
     5761 gccaatagca attaaactat ttgctaactg atagctagat ccatttggat ctaaaaatgg
     5821 attcaaccat gagtctattc ttttaaactg atagtcgtga aatcctattt ttaataagaa
     5881 atctcttcct gtatctgata taaccaaata taaaaatatt aaggataaaa caacaccaat
     5941 ggcgattatt ggggcaatta tttttatatt tatgttagac gtaaacaaaa taaaaatata
     6001 aataacaatg aagactaaca ttgttcctag atcgttctga aaaaataata atattcctat
     6061 tggaagagta gcaataaaag ttttaagtaa ataaatccaa tcatctttcg tattactagt
     6121 aatagatttt tgctgataat tgacggttat tttagctagc ataagggtgt atcctatttt
     6181 gactaactca gtaggttgaa acgaaaaacc agcaatataa aaccaatttt ttgctccagt
     6241 taagactgct aattgtggat tatagaattt caagagaagt aataacatta tgatgcataa
     6301 tccgtaaaat ataggagtta atttccacaa aattttagga ttaaaaaaat aaatagttat
     6361 tgctgaagtg aacccaatac tataccacaa tacttgttta attaattctt ttgtgatttt
     6421 tgaattagtg taagagtcgt gatataaagc aacatataaa gaaagtaatc caatgataca
     6481 tagcagaaaa actggcaata aaagcccata attcaactga tttttgtaga ttctattatt
     6541 tttcatttcg aaaggtcctt tttcataatt tataaataaa aataacacaa taaagttatt
     6601 tttactagtt ttgagtttta gacgaaaatt acaattagct acttttttat cttactaaat
     6661 agttctatct ttttatataa agtatagaaa aattaataaa acaattggaa atatgctgtg
     6721 aagaaataag tttctatgtt tattaaagat aataattaaa gtattaatta ttaatgtatt
     6781 aattattaat agtaaactta gaaaaaatat taataaccaa gtggcaattg aatggctaaa
     6841 ataaaagaat ccggactgaa ttaaggtgaa aactaaactt aaaaaaatta caaaatgaaa
     6901 taatagtatt agtgtttttg tatgattttt taccataatt ttttaccata attttttctt
     6961 ctcctaacaa aatattgtct agtgataaat caaataactt agcaaactga attaaatttt
     7021 ctgtatctgg tattgttcga ccattttccc aattagaaat ggttttttta gatactaaaa
     7081 gcatttcagc taaatcttct tgagttagtt gtttttctat tcttttttgt ttgattactt
     7141 tacttatagt catttgatca cctaaacata ttttttattc ttagaaagat agagtctata
     7201 gaaattgatt tcttctttat ggaaagttgg taattgttac caaaatttcc accacttttt
     7261 ttctgttgtt attgcattgt tgttttcttt attgacagaa gttaattcct cttcttcagg
     7321 ttcttccgta taagctaatt gtaactgttc ttgcagtttt tcgatctgtt gatttgcctg
     7381 caaggtgagt tgttgttgct ggtcgagtaa cttttgcatt tgttttattt gagtatcttt
     7441 ttcttggagc tgttcatgta aaaaaacaat aatcgagttt tcatcgttac tagcaacggt
     7501 ttctacttgg ttaccagtgg tgttactacc taaaaaatgt tgttttaaaa gcaaatatcc
     7561 tttgtagctg acaactaagg ttttcactcc gttcctagta acagtttgca catagttttc
     7621 tctaaaatca gctgatagcc ttttcctaat tgcttgtttt gaaacatgaa attcttccgc
     7681 gagttctttg atcgttttat ctatcataaa tactccttat gcttcaatcg aataagtttt
     7741 gtaagttttc taataaatct tgtttaagct catttgagat tgattgtttt tccgcttctt
     7801 ctttttcggt ttgttgttgt tttttaaaat cagcttcatg agtacctaac ggaagattta
     7861 gtattcgatc tttagcacgc catttttctt tttgtgttaa ttctccattg tgttcaatat
     7921 taaataattt tatacgtaaa tcttcttgct tcccttttga aaaatcgtca gcatggttga
     7981 tttcagcttt ccaagtaaaa cgataaccaa taacaggttt acctcgacct ttaccatatt
     8041 ttttacgaat agttaatcct ttaaatagtg gagttagttc ttcttttatt ggtttgagta
     8101 caaactgatt tatatttgtt tgtctatagc ttttaggaat atctaatagc tctaaaaaat
     8161 cttccttaga aaattcagcc catccttgag ttcggtattg ttttagcaat ctaaacattg
     8221 ttttagcata actgctattt aaatctcgaa attctgttaa agagaaacgt acccagcttt
     8281 ctagattatt aagtaaaggc aaagcttttt cgtggacttt tatatctaca taagggttat
     8341 cgacttctcc atgaatttca aaatcagtaa acattgtaaa tcgttctatt gttaatccac
     8401 cattgctttt tcttccgaaa cgtaaatcca tcaaatgatc gtatgttctt gccaaatctt
     8461 ttataaagac ttctcctgca gtctgcttat acgcacttaa atctttaagt tggctgaaat
     8521 aaaagcgtat tttttgatta cctttatcgc gcattctaga gacaatagaa aaaaacaagt
     8581 tcatttctat tggggtaaat tttcttaaag gaatggtatt taattctggt tcatacttta
     8641 cgatttcatt tgccatttag ttgttactcc agtctaaatg attttattga catcatttta
     8701 ggacatatta tgtctaaaaa caatacctaa aacagataat ttcatgccta taaacagata
     8761 atttcatgcc tataaacaga taatttcatg cctataaaca gataatttca tgcctataaa
     8821 attttgtata ccttggggga gtaaggacac agccgacgtt aaagaggttt aaaaagaggt
     8881 ttaaaaaaag attataaaag agggagacaa aatttcccac ctaaaaaaac aattattttt
     8941 tctttaaaca ggttcgcgaa cttttctaaa ctgctaattc aaaacctcgc tccctacggt
     9001 cgccaaaaag gtgtgatcgc tacgctctca cattatacct gcgcagtcgc ctccagctcc
     9061 tttgctaaaa ccattgttca aaaaggaggg ttgaggctct aagagcttgt ctaagccgtt
     9121 ttaagacagt cttagtataa aaaactttat ctggaattta aagcgcccta caggcggtca
     9181 gggcaagccc taacaacccg gaaataagag atacttaaaa ttgccacaca gatttaattt
     9241 tgtagggtat aataacttgt cgcctatccg acagtcttgc gactggaagg aggtgtatct
     9301 gatgtttgaa ctgtttattt gcccccttct tgtaggagtt tgtatctctg tattcagtta
     9361 ttggttgaat aaccgaaaca agaagtaaaa tcagtaaagg cgacatcaac ctaaaaaaag
     9421 ccccagagaa tccgacctct ctggggcttt cgtgtatcta atgtttgaac atttgcatgt
     9481 ttagtttaac atgaatttca atcaaattca attttgctgt tgctgatcta accattcctt
     9541 gacgcttttt aaataattta aagctgtttc ttttttagtc agattttgta tgttaattgc
     9601 ttgttgaagc aagatttcat agcgttcata tgtgactgat tgttgtcgtt cttttcgttc
     9661 ttgttcgcta gttgaaaaag gaattgtatc tccaaataaa tcactacaag cttcttccac
     9721 tttcgtatct ttcacatatt gcacgtcttt tttggttcgc tcatagttgt cttgaacctc
     9781 tttttgctgt ttttctggca agttttgata aagacgagtg agtttttcca tgtttttatc
     9841 cgtcaaacgt ttttcttgat tcacaaaaat atcgttcatt tcctcataac aagtggtaaa
     9901 ctcttccatt ttttgtgcta atgcttgctc ttttttcttt ttatcacgcg ctgattcata
     9961 cttttgagtg acatttccta ataaaatact tctagaaacg cgcttatttc cctctaaaac
    10021 gccgaataag ccgcttgaaa cgattatcaa ggaaaatata cagccaagaa agaaaaatcg
    10081 cttaaatcga attttagagc cttttaatga atatcttgtt tgaaaaattt tatagatgag
    10141 ccaaatactt cctgaaatac taacaaaaat gagtagatag ggtaaataaa atgccatttt
    10201 agcctcccca tactaaccaa aatgcgaaca gtgtcaggac taagcaacat aagggggcaa
    10261 gaaaatccaa tgatcgttga ataagcacct ttttctgttc cttatttcgc tggtctaata
    10321 gctcttgatg gagtttttta acttgtaaat caatcgtagt ttgtatcctt tcttgcgtat
    10381 aaggaacgag aaaagcacgt tgtttcttta tttcgttttc tatgtctttt aacgttttag
    10441 agcgataatt cttgttggtg gtctgttcct ctttcaacgt gttgttcatg gtttcttcga
    10501 gccggtctaa tttccggttg ttcaggcgta agagttcgtt gttctcttcg tacttcttct
    10561 gcagtttcgc gaatgctagc tcgagctctt cgtagctcgt tggttcgctg ttggttatag
    10621 gttcgttctc gtctgtccag ctcatgtaaa atcgtctcct tttcgtagtc ctttcctagt
    10681 ctgttggatc gtactttttt ctttttgtgg atcaattcgt aagtgaaatt tttcccgcga
    10741 ttgaagcaat gaatccccaa tgttttgagg gaattacaaa attggtcata ggttctggtt
    10801 tttttgtttt tcattgtttg atcgatcgca atccgaattt cgtctttcca aacaggttca
    10861 tttttttctt ttaattttat ttcagacatg gttcgttttt ctggtttctt tcctcgctca
    10921 gtgattggta agttatgtgc aagacaaatc tcgtcattta ggtcataaca tttgtttaga
    10981 aatgcttgac catgagcttg aaattttttt ccagtctcca gattaacgga gttaatgaca
    11041 atgtgattat gaatatggtc tttgtctgta tgtgtgtaaa tagctatttg gtgatcgggg
    11101 gctatttttt ttgcatactc tgcccctagt tgattagctt tttcagcggt aatttctcct
    11161 ggtcgaaaag attgaatcaa taagtgtgcc tgtacacggt cgtcttttcc atacagcatg
    11221 cgaatttgtt tcatttcact tttggcataa tcgacgtcac agtttattcc tgttttaatg
    11281 gttgctcgtg gttctgcgta attaattgca cgactacaag aagtggttcg gcttacctta
    11341 attgttgcca tatcttttgt agctcctttt gcatgatttt taggttttcg gataaacgtt
    11401 gagcgtcacc agaagcaacg tcatgttggt gtgtattgca ccacttagca atttgattga
    11461 tattccctcc agctttggct aaatcgcgcg cgattgcctg ggcaatgtct ggcgccactt
    11521 tcggtgtcac cagacgcgcg ttttgtgcct tggctttcac gaaagccggc acactcattt
    11581 gcaaagcttc agccgattgc ttcagctttg caaattcgga ttcgctcact cgaaaactga
    11641 tttgttttgg ctcggatcga taggtatatt ttttagaaga attgctagcc aaattttgat
    11701 tttgttcgct cataagtccc accctttcag tacgtcctac ggacgtatcg ttcgcaatac
    11761 cattgtacct aaaaaggtac aatgagtaaa gctcactaaa agggtttggc aagctctata
    11821 tttgctagcc aaatacctac gtatttagtg caaataaccg cttgtcaaag gggcgcaagc
    11881 tcccccttga atccccctca aaagccacgc tccacaatac aaaaaaagct agcaaatgct
    11941 agccactttt tgtaaagttt cgcttatttc accgaagatg aaattgttgc tttgcaacaa
    12001 aaaacataaa tgcttttttg tgtaaactac taactattta gctaaaactg atagttttat
    12061 taaacattca gcaatatttt tagccaattt tttatcagac tttcggcgag tcattatgat
    12121 aatatgtcca ctttttgtta cttctaccaa aaatctagca ataactctcc cattcttcag
    12181 ttgatttagt ttcataagtc ttttgtaatt gacggtttaa tttttttggc aattgcaaag
    12241 aataaataag tctatcataa aatttttcat actctttttt caataaaatc acttgggtgc
    12301 ctccttttta ttaagtgatt atttaaattt tgggatagga gagatggaat ataaaataaa
    12361 tacccgtggt gctagtttta aatcagctag catcacgggt aaaaattata attctataat
    12421 attagttcca tagtttagaa catttaagtc atgtgttact agtacaacgg cttttttttc
    12481 tttttctgtt aaatttttca gaaagttcat tataaaagtt gagttttctt tatctaaatt
    12541 tcctgtaggt tcatctgcaa ataatatatc aaatggtttt aacatatttc taattaatgc
    12601 tattctttgt tgttctccac cgcttaagag aaagattttt tcttttaaga tatcttttgg
    12661 caaagaaaaa tctgttaata aattataaat atgatcattg gaaatattac tgttaaactt
    12721 ttttgctaat tcgaaattgt attgtactgt ttgattttca agaagagcat agttttgaaa
    12781 taaatagcta atatgatttc ttcgtagacg ttgtatatca tgtttagaaa ctttcttttc
    12841 aaaatagaat aagttaccgg tatattgttt atctattagg cttaaattat ttaacagagt
    12901 agtttttcca ctaccacttt ttcctgttac tataataaaa tctttttctg atattgaaaa
    12961 gttccaattt tgtttgatct tttttttatt aattgacagg gtgaaattct caactgataa
    13021 tatcataaaa tattctcctt aaaatattta aaactatttt ttttataaat aataatagaa
    13081 gtaattagat ttattaaaaa gatcataaaa attataaaat aca
//
