LOCUS       NZ_CP012198             8401 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli strain ECwhn14 plasmid p8401, complete sequence.
ACCESSION   NZ_CP012198
VERSION     NZ_CP012198.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03943546
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Lei,C.W., Zhang,A.Y. and Wang,H.N.
  TITLE     Genomic insight into acquired antimicrobial resistance determinants
            in a multidrug resistant Escherichia coli isolate from healthy
            chicken in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Lei,C.W., Zhang,A.Y. and Wang,H.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2015) School of Life Science, Sichuan University,
            No.29 Wangjiang Road, Chengdu, Sichuan 610064, China
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: DNAstar v. 7.0
            Sequencing Technology             :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009948825.1-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 02:47:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP012198.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ECwhn14"
                     /isolation_source="Chicken"
                     /db_xref="taxon:562"
                     /plasmid="p8401"
                     /geo_loc_name="China: Hebei"
                     /lat_lon="38.52 N 115.31 E"
                     /collection_date="2014-10-18"
                     /collected_by="Hong-Ning Wang"
                     /genotype="ST167"
     gene            200..442
                     /locus_tag="AKG29_RS00010"
                     /old_locus_tag="AKG29_01315"
     CDS             200..442
                     /locus_tag="AKG29_RS00010"
                     /old_locus_tag="AKG29_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(474..1124)
                     /gene="tetR(A)"
                     /locus_tag="AKG29_RS00015"
                     /old_locus_tag="AKG29_01320"
     CDS             complement(474..1124)
                     /gene="tetR(A)"
                     /locus_tag="AKG29_RS00015"
                     /old_locus_tag="AKG29_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_001493764.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEADPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            1230..2429
                     /gene="tet(A)"
                     /locus_tag="AKG29_RS00020"
                     /old_locus_tag="AKG29_01325"
     ARG             1231..2426
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="99.75"
                     /coverage="100.0"
     gene            complement(<2396..2584)
                     /locus_tag="AKG29_RS00025"
                     /old_locus_tag="AKG29_01330"
                     /pseudo=""
     CDS             complement(<2396..2584)
                     /locus_tag="AKG29_RS00025"
                     /old_locus_tag="AKG29_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(2628..3050)
                     /locus_tag="AKG29_RS00030"
                     /old_locus_tag="AKG29_01335"
     CDS             complement(2628..3050)
                     /locus_tag="AKG29_RS00030"
                     /old_locus_tag="AKG29_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            3235..3675
                     /locus_tag="AKG29_RS00035"
                     /old_locus_tag="AKG29_01340"
     CDS             3235..3675
                     /locus_tag="AKG29_RS00035"
                     /old_locus_tag="AKG29_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(4442..4591)
                     /locus_tag="AKG29_RS00040"
     CDS             complement(4442..4591)
                     /locus_tag="AKG29_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            5162..5320
                     /locus_tag="AKG29_RS00070"
                     /old_locus_tag="AKG29_01360"
     CDS             5162..5320
                     /locus_tag="AKG29_RS00070"
                     /old_locus_tag="AKG29_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            5780..6595
                     /gene="sul2"
                     /locus_tag="AKG29_RS00055"
                     /old_locus_tag="AKG29_01365"
     ARG             5781..6592
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6656..7459
                     /gene="aph(3'')-Ib"
                     /locus_tag="AKG29_RS00060"
                     /old_locus_tag="AKG29_01370"
     ARG             6657..7456
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7459..8295
                     /locus_tag="AKG29_RS00065"
                     /old_locus_tag="AKG29_01375"
     CDS             7459..8295
                     /locus_tag="AKG29_RS00065"
                     /old_locus_tag="AKG29_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     ARG             7460..8292
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(4627..4800)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          4724..5329
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tggaagcccc gtagcgacgc ggagaggggc gagacaagcc aagggcgcag gctcgatgcg
       61 cagcacgaca tagccggttc tcgcaaggac gagaatttcc ctgcggtgcc cctcaagtgt
      121 caatgaaagt ttccaacgcg agccattcgc gagagccttg agtccacgct agatctatct
      181 catctgcgca aggcagaacg tgaagacggc cgccctggac ctcgcccgcg agcgccaggc
      241 gcacgaggcc ggcgcgcgga cccgcgccac ggcccacgag cggacgccgc agcaggagcg
      301 ccagaaggcc gccagagagg ccgagcgcgg ccgtgaggct tggacgctag ggcagggcat
      361 gaaaaagccc gtagcgggct gctacgggcg tctgacgcgg tggaaagggg gaggggatgt
      421 tgtctacatg gctctgctgt agtgagtggg ttgcgttccg gcagcggtcc tgatcaatcg
      481 tcaccctttc tcggtccttc aacgttcctg acaacgagcc tccttttcgc caatccatcg
      541 acaatcaccg cgagtccctg ctcgaacgct gcgtccggat cggcttcgtc gaaggcgtct
      601 atcgcggccc gcaacagcgg cgagagcgga gcctgttcaa cggtgccgcc gcgctcgccg
      661 gcatcgctgt cgccggcctg ctcctcaagc acggccccaa cagtgaagta gctgattgtc
      721 atcagcgcat tgacggcgtc cccggccgaa aaacccgcct cgcagaggaa gcgaagctgc
      781 gcgtcggccg tttccatctg cggtgcgccc ggtcgcgtgc cggcatggat gcgcgcgcca
      841 tcgcggtagg cgagcagcgc ctgcctgaag ctgcgggcat tcccgatcag aaatgagcgc
      901 cagtcgtcgt cggctctcgg caccgaatgc gtatgattct ccgccagcat ggcttcggcc
      961 agtgcgtcga gcagcgcccg cttgttcctg aagtgccagt aaagcgccgg ctgctgaacc
     1021 cccaaccgtt ccgccagttt gcgtgtcgtc agaccgtcta cgccgacctc gttcaacagg
     1081 tccagggcgg cacggatcac tgtattcggc tgcaactttg tcatgcttga cactttatca
     1141 ctgataaaca taatatgtcc accaacttat cagtgataaa gaatccgcgc gttcaatcgg
     1201 accagcggag gctggtccgg aggccagacg tgaaacccaa cagacccctg atcgtaattc
     1261 tgagcactgt cgcgctcgac gctgtcggca tcggcctgat tatgccggtg ctgccgggcc
     1321 tcctgcgcga tctggttcac tcgaacgacg tcaccgccca ctatggcatt ctgctggcgc
     1381 tgtatgcgtt gatgcaattt gcctgcgcac ctgtgctggg cgcgctgtcg gatcgtttcg
     1441 ggcggcggcc ggtcttgctc gtctcgctgg ccggcgctgc tgtcgactac gccatcatgg
     1501 cgacgacgcc tttcctttgg gttctctata tcgggcggat cgtggccggc atcaccgggg
     1561 cgactggggc ggtagccggc gcttatattg ccgatatcac tgatggcgat gagcgcgcgc
     1621 ggcacttcgg cttcatgagc gcctgtttcg ggttcgggat ggtcgcggga cctgtgctcg
     1681 gtgggctgat gggcggtttc tccccccacg ctccgttctt cgccgcggca gccttgaacg
     1741 gcctcaattt cctgacgggc tgtttccttt tgccggagtc gcacaaaggc gaacgccggc
     1801 cgttacgccg ggaggctctc aacccgctcg cttcgttccg gtgggcccgg ggcatgaccg
     1861 tcgtcgccgc cctgatggcg gtcttcttca tcatgcaact tgtcggacag gtgccggccg
     1921 cgctttgggt cattttcggc gaggatcgct ttcactggga cgcgaccacg atcggcattt
     1981 cgcttgccgc atttggcatt ctgcattcac tcgcccaggc aatgatcacc ggccctgtag
     2041 ccgcccggct cggcgaaagg cgggcactca tgctcggaat gattgccgac ggcacaggct
     2101 acatcctgct tgccttcgcg acacggggat ggatggcgtt cccgatcatg gtcctgcttg
     2161 cttcgggtgg catcggaatg ccggcgctgc aagcaatgtt gtccaggcag gtggatgagg
     2221 aacgtcaggg gcagctgcaa ggctcactgg cggcgctcac cagcctgacc tcgatcgtcg
     2281 gacccctcct cttcacggcg atctatgcgg cttctataac aacgtggaac gggtgggcat
     2341 ggattgcagg cgctgccctc tacttgctct gcctgccggc gctgcgtcgc gggctttgga
     2401 gaaattcttc aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa
     2461 gcgcagcgaa tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc
     2521 ggtggctctg gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag
     2581 gcatgttccg cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt
     2641 cttttaccct cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact
     2701 cccactctta cattgttcgc agtatctagg tacatgttat tgcgtgcacc ttcatgatgt
     2761 ataatgttat taatgacagc agaaagatcg acagaattaa tattcttcgt acctgggaca
     2821 gttatcgggt gtcggccagt gatgagacca aaggatgcag tataagatga accactacta
     2881 ttatcatggc caccattacc accaccgtta aaactacctc ccgaacttcc agagccgcta
     2941 tgaccatcat tgtaggggtc atttggtccc atgtgaccaa aaccaccgac agtaccgcta
     3001 tttaactctg tactcataaa attatcctct ttgactttta aaaacagcaa aaactgtatt
     3061 tataacaatg tgtttatata cagtattcga aatactacgc ccgtcaaaac cacaccgtca
     3121 acaggttttt tgtgtatttt tcacgcctca cgggcagcct cagacctttt gtgttaaaac
     3181 agtaatatgt tctgctgtta caatacggta cacacaggag acactgtcac ttttatggca
     3241 tggcacacag ttcaagagac actgaaatta acaggaaagt cgcgctcgca attatatcgt
     3301 gacatggcta aaggccttgt cagctatcgc acaggaaaaa atgatcgccg ggagttcgag
     3361 accagtgaac tgattcgggc ctatggcgag ctgcgcactt ctgagacacc aaaaaggcac
     3421 aaggagggac actccaaatc tgtcgttagc caggcggaaa atgctcagct agacgtcatt
     3481 cagcagaaac tggacgctct gcagcagact gttaccctaa tgcttgaaga caagatggcg
     3541 cgggaagcag aaaaccgcct gcgaaatgag gagagggaac aactacaggc cgaaattagc
     3601 cgtctggctg acgcgctggc acaggaaaaa aagcggggat tctggtcgcg gctgtttcgc
     3661 tccggttcag agtaacagtg caggtgactc tgatttacca taacatctgt tatcagcacc
     3721 gcctgagagg ccctcagggc gtttctgacg ggaaaccggt aaaacaggca acaaaccacc
     3781 cgaaaacccg ccagaaacgc gcaggctctg tttttacggc atgcatgagt atgcgcacaa
     3841 gtgcatagcg cggaaattca caccattcag ccggggtaag tcctgtttcc ggcgttcttc
     3901 ccatccgaaa acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc gttgccggcg
     3961 cagccggaaa acccgctgtc agcacgttca ggaagggggt gatgtacaaa tggcacagag
     4021 gatgaaagtg acgtatgagc tgaaggaatg atgtaaaagt gatgtataag tgatgtataa
     4081 gtgatgtata agtgatgtaa aagtgatgta tgaactgaag gagcgatgta attctgtagt
     4141 caggaagaat aacccgttca tgcaggcatg aacgatacag tgaggaacgc tttttttgtc
     4201 acgatctccc cttaacctgc gtaaatcacc cgccatggtt gattattttt caacctcaaa
     4261 atggtcatac acctaacgca gccgcgattt tcatcgcaac tgcatgaatt aaaagaaaaa
     4321 aaaggaatat ttcgttttca cgagtaatac ccgggtatta aaaatccact gtaattacat
     4381 gaaaataaag aaataaaagg taataccgga gtattaccac gtacgcgggc aagcgaaaaa
     4441 gctataacgc ttcccggccg acaggcgacc caaaatccac aggggcatgc aggttctctt
     4501 cagtaacctg cgccattaaa tcaaaaaggt catattcgcc gggaagctgc gtatcagtcg
     4561 aatccagttg ttcatcatat tcagatggca tggggtgata tccagtagct gagaggttgt
     4621 tagcgggttt cggggcgcag ccctgaacca gtcatgtagc gctagcggag tgtatactgg
     4681 cttactatgt tgccacgatt gcgaaaatca gtgaagtgct tcatgtggca ggagaaaaaa
     4741 ggcagcaccg gcgcgtcagc agaatatgcg atacaggaga tattccgctt cctcgctcac
     4801 tgactcgcta cgctcggtcg ttcggctgcg gcgagcggta ccggcttacg gacggggacg
     4861 gaaatttcct ggaagatacc aggaagacac ttagcaaaga agagataagg ccacggcgaa
     4921 gccgtttttc cataggctcc gcccccctga caagcatcgc aaaatctgac gctcaaatca
     4981 gtggtggcga aacccgacag gactataaag atcccaggcg tttccccctg gtagctccct
     5041 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     5101 gtttctcatt ccacgcctga cactcagttc cgtgaaggca gttcgctcca agctggactg
     5161 tatgcacgaa ccccccgttc agtccgaccg ctgcgccttg tccggtaact atcaacttga
     5221 gtccaacccg gaaagacacg acaaaacgcc actggcagca gccattagta acaggagtgc
     5281 acgagaagat agattaagag gtcttgaagt agaggcctga ctgcggctac actgaaagaa
     5341 cagttttggt aatggcggtc tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga
     5401 cttaaccttc gaacaaaccg cctccccagg cggttttttc gtttacagag caagagatta
     5461 cgattcattg aaaaggatct caagaagatc ctttgtagat tactatcaat taatcttccg
     5521 aacatgacac cagtcactgt tgattacggt taaaccgctc cgtaacaggt ttccctcgta
     5581 ttggtgactt gcagcaccaa agaagcaaaa aagataaata tatctactta ttgagagatg
     5641 atatatttat ttccaaaatg ctacagatac atcatcatga aacggatcac cgcaaacagg
     5701 ttactcgcct ggggattccc tttcgacccg agcatccgta tgagactcat gctcgattat
     5761 tattattata gaagccccca tgaataaatc gctcatcatt ttcggcatcg tcaacataac
     5821 ctcggacagt ttctccgatg gaggccggta tctggcgcca gacgcagcca ttgcgcaggc
     5881 gcgtaagctg atggccgagg gggcagatgt gatcgacctc ggtccggcat ccagcaatcc
     5941 cgacgccgcg cctgtttcgt ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc
     6001 gctcaaggca gatggcattc ccgtctcgct cgacagttat caacccgcga cgcaagccta
     6061 tgccttgtcg cgtggtgtgg cctatctcaa tgatattcgc ggttttccag acgctgcgtt
     6121 ctatccgcaa ttggcgaaat catctgccaa actcgtcgtt atgcattcgg tgcaagacgg
     6181 gcaggcagat cggcgcgagg cacccgctgg cgacatcatg gatcacattg cggcgttctt
     6241 tgacgcgcgc atcgcggcgc tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga
     6301 tcccggcatg gggttttttc tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg
     6361 gttcgatgaa ttgcggctgc gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc
     6421 ctttctgcgc gcgctcacag gccgtggtcc gggggatgtc ggggccgcga cactcgctgc
     6481 agagcttgcc gccgccgcag gtggagctga cttcatccgc acacacgagc cgcgcccctt
     6541 gcgcgacggg ctggcggtat tggcggcgct gaaagaaacc gcaagaattc gttaactgca
     6601 cattcgggat atttctctat attcgcgctt catcagaaaa ctgaaggaac ctccattgaa
     6661 tcgaactaat attttttttg gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga
     6721 atctggtgat tttgtttttc gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc
     6781 ttcccgccgc ggtgagctcg ctggagagcg tgaccgcctc atttggctca aaggtcgagg
     6841 tgtggcttgc cccgaggtca tcaactggca ggaggaacag gagggtgcat gcttggtgat
     6901 aacggcaatt ccgggagtac cggcggctga tctgtctgga gcggatttgc tcaaagcgtg
     6961 gccgtcaatg gggcagcaac ttggcgctgt tcacagccta tcggttgatc aatgtccgtt
     7021 tgagcgcagg ctgtcgcgaa tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt
     7081 caatcccgac ttcttaccgg acgaggacaa gagtacgccg cagctcgatc ttttggctcg
     7141 tgtcgaacga gagctaccgg tgcggctcga ccaagagcgc accgatatgg ttgtttgcca
     7201 tggtgatccc tgcatgccga acttcatggt ggaccctaaa actcttcaat gcacgggtct
     7261 gatcgacctt gggcggctcg gaacagcaga tcgctatgcc gatttggcac tcatgattgc
     7321 taacgccgaa gagaactggg cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt
     7381 caatgtattg gggatcgaag cccccgaccg cgaacgcctt gccttctatc tgcgattgga
     7441 ccctctgact tggggttgat gttcatgccg cctgtttttc ctgctcattg gcacgtttcg
     7501 caacctgttc tcattgcgga caccttttcc agcctcgttt ggaaagtttc attgccagac
     7561 gggactcctg caatcgtcaa gggattgaaa cctatagaag acattgctga tgaactgcgc
     7621 ggggccgact atctggtatg gcgcaatggg aggggagcag tccggttgct cggtcgtgag
     7681 aacaatctga tgttgctcga atatgccggg gagcgaatgc tctctcacat cgttgccgag
     7741 cacggcgact accaggcgac cgaaattgca gcggaactaa tggcgaagct gtatgccgca
     7801 tctgaggaac ccctgccttc tgcccttctc ccgatccggg atcgctttgc agctttgttt
     7861 cagcgggcgc gcgatgatca aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt
     7921 atagccgatc aaatgatgag caatgcctcg gaactgcgtg ggctacatgg cgatctgcat
     7981 catgaaaaca tcatgttctc cagtcgcggc tggctggtga tagatcccgt cggtctggtc
     8041 ggtgaagtgg gctttggcgc cgccaatatg ttctacgatc cggctgacag agacgacctt
     8101 tgtctcgatc ctagacgcat tgcacagatg gcggacgcat tctctcgtgc gctggacgtc
     8161 gatccgcgtc gcctgctcga ccaggcgtac gcttatgggt gcctttccgc agcttggaac
     8221 gcggatggag aagaggagca acgcgatcta gctatcgcgg ccgcgatcaa gcaggtgcga
     8281 cagacgtcat actagatatc aagcgacttc tcctatcccc tgggaacaca tcaatctcac
     8341 cggagaatat cgctggccaa agccttagcg taggattccg ccccttcccg caaacgaccc
     8401 c
//
