LOCUS       NZ_CP011984            13838 bp    DNA     circular CON 02-OCT-2023
DEFINITION  Klebsiella pneumoniae 500_1420 plasmid p500_1420-13.838kb, complete
            sequence.
ACCESSION   NZ_CP011984
VERSION     NZ_CP011984.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02141993
            Assembly: GCF_000406765.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae 500_1420
  ORGANISM  Klebsiella pneumoniae 500_1420
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 13838)
  AUTHORS   Wright,M.S., Perez,F., Brinkac,L., Jacobs,M.R., Kaye,K., Cober,E.,
            van Duin,D., Marshall,S.H., Hujer,A.M., Rudin,S.D., Hujer,K.M.,
            Bonomo,R.A. and Adams,M.D.
  TITLE     Population structure of KPC-producing Klebsiella pneumoniae isolates
            from midwestern U.S. hospitals
  JOURNAL   Antimicrob Agents Chemother 58 (8), 4961-4965 (2014)
   PUBMED   24913165
REFERENCE   2  (bases 1 to 13838)
  AUTHORS   Adams,M., Sanka,R., Wright,M., Brinkac,L., Perez,F., Jacobs,M.,
            Kaye,K., Cober,E., Duin,D., Marshall,S., Hujer,A., Rudin,S.,
            Hujer,K. and Bonomo,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2015) JCVI, 9704 Medical Center Drive, Rockville,
            MD 20850, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Assembly Name                     :: Klebsiella pneumoniae 500_1420
            Expected Final Version            :: Yes
            Genome Coverage                   :: 15.35x
            Sequencing Technology             :: PacBio RS
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/02/2023 01:34:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,425
            Genes (coding)                    :: 5,295
            CDSs (with protein)               :: 5,295
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 70 of 130
            Pseudo Genes (incomplete)         :: 71 of 130
            Pseudo Genes (internal stop)      :: 17 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP011984.1.
            This strain is part of the Klebsiella pneumoniae Genome Sequencing
            Center Project as part of the Genome Sequencing Centers for
            Infectious Diseases contract.
            http://gsc.jcvi.org/projects/gsc/klebsiella_pneumoniae/index.php.
            Source DNA and/or bacteria are available from the laboratory of Dr.
            Robert Bonomo, Cleveland VA Medical Center, 19701 East Boulevard,
            Cleveland, OH 44106, USA.  This project has been funded in whole or
            in part with federal funds from the National Institute of Allergy
            and Infectious Diseases, National Institutes of Health, Department
            of Health and Human Services through the Genomic Sequencing Centers
            for Infectious Diseases under contract number HHSN272200900007C.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13838
                     /organism="Klebsiella pneumoniae 500_1420"
                     /mol_type="genomic DNA"
                     /strain="500_1420"
                     /db_xref="taxon:1304922"
                     /plasmid="p500_1420-13.838kb"
     gene            complement(492..641)
                     /locus_tag="J052_RS28060"
                     /old_locus_tag="J052_28100"
     CDS             complement(492..641)
                     /locus_tag="J052_RS28060"
                     /old_locus_tag="J052_28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(726..983)
                     /locus_tag="J052_RS28065"
                     /old_locus_tag="J052_28105"
     CDS             complement(726..983)
                     /locus_tag="J052_RS28065"
                     /old_locus_tag="J052_28105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(986..1813)
                     /locus_tag="J052_RS28070"
                     /old_locus_tag="J052_28110"
     CDS             complement(986..1813)
                     /locus_tag="J052_RS28070"
                     /old_locus_tag="J052_28110"
                     /inference="COORDINATES: protein motif:HMM:NF023002.3"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]; GO:0043022 - ribosome binding
                     [Evidence IEA]; GO:0003723 - RNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /protein_id="WP_260690937.1"
                     /translation="MSFRSTERQTPTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDK
                     AKAELDAEDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEY
                     GSGWQDQVRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKD
                     AENKLNEEKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYG
                     DKKRKIYEWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPIQNETLKNIFKR"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            complement(1929..>2099)
                     /locus_tag="J052_RS29640"
                     /pseudo=""
     CDS             complement(1929..>2099)
                     /locus_tag="J052_RS29640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /name="serine hydrolase"
     gene            complement(2154..2858)
                     /locus_tag="J052_RS28075"
                     /old_locus_tag="J052_28115"
     CDS             complement(2154..2858)
                     /locus_tag="J052_RS28075"
                     /old_locus_tag="J052_28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(<2924..3139)
                     /locus_tag="J052_RS28080"
                     /old_locus_tag="J052_28120"
                     /pseudo=""
     CDS             complement(<2924..3139)
                     /locus_tag="J052_RS28080"
                     /old_locus_tag="J052_28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /GO_function="GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing protein"
                     /name="nucleotidyltransferase domain-containing protein"
     gene            complement(3209..3763)
                     /gene="aac(6')-Ib"
                     /locus_tag="J052_RS28085"
                     /old_locus_tag="J052_28125"
                     /pseudo=""
     CDS             complement(3209..3763)
                     /gene="aac(6')-Ib"
                     /locus_tag="J052_RS28085"
                     /old_locus_tag="J052_28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003159191.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /name="aac(6')-Ib"
     gene            complement(3997..4554)
                     /locus_tag="J052_RS30930"
                     /old_locus_tag="J052_28130"
                     /pseudo=""
     CDS             complement(3997..4554)
                     /locus_tag="J052_RS30930"
                     /old_locus_tag="J052_28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     gene            4717..>7690
                     /locus_tag="J052_RS29670"
                     /pseudo=""
     CDS             4717..>7690
                     /locus_tag="J052_RS29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            <7758..8046
                     /locus_tag="J052_RS28120"
                     /old_locus_tag="J052_28155"
                     /pseudo=""
     CDS             <7758..8046
                     /locus_tag="J052_RS28120"
                     /old_locus_tag="J052_28155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /name="Exc2 family lipoprotein"
     gene            8351..8613
                     /locus_tag="J052_RS28125"
                     /old_locus_tag="J052_28160"
                     /pseudo=""
     CDS             8351..8613
                     /locus_tag="J052_RS28125"
                     /old_locus_tag="J052_28160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /name="plasmid stabilization protein"
     gene            8603..8901
                     /locus_tag="J052_RS28130"
                     /old_locus_tag="J052_28165"
                     /pseudo=""
     CDS             8603..8901
                     /locus_tag="J052_RS28130"
                     /old_locus_tag="J052_28165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8964..9340)
                     /locus_tag="J052_RS28135"
                     /old_locus_tag="J052_28170"
                     /pseudo=""
     CDS             complement(8964..9340)
                     /locus_tag="J052_RS28135"
                     /old_locus_tag="J052_28170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /name="entry exclusion protein 1"
     gene            9440..9589
                     /locus_tag="J052_RS28140"
                     /old_locus_tag="J052_28175"
     CDS             9440..9589
                     /locus_tag="J052_RS28140"
                     /old_locus_tag="J052_28175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_052536225.1"
                     /translation="MAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDAETLWQVMRDK
                     LGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(10152..10883)
                     /locus_tag="J052_RS28150"
                     /old_locus_tag="J052_28185"
     CDS             complement(10152..10883)
                     /locus_tag="J052_RS28150"
                     /old_locus_tag="J052_28185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(10883..12847)
                     /locus_tag="J052_RS28155"
                     /old_locus_tag="J052_28190"
     CDS             complement(10883..12847)
                     /locus_tag="J052_RS28155"
                     /old_locus_tag="J052_28190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     ARG             complement(2871..3127)
                     /name="aadA15"
                     /gene="aadA15"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.14"
                     /coverage="32.32"
     ARG             complement(3210..3613)
                     /name="AAC(6')-30/AAC(6')-Ib' bifunctional protein"
                     /gene="AAC(6')-30/AAC(6')-Ib' bifunctional protein"
                     /gene_name="aminoglycoside bifunctional resistance protein"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="98.51"
                     /coverage="40.85"
     Typing          complement(30..228)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.109"
                     /coverage="99.0"
                     /evalue="1.87e-63"
     Typing          complement(10156..10883)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.34e-150"
ORIGIN
        1 ttgcgctctc ctgttccaac cctgccgttt cccggatacc tgttccgcct ttctcccttg
       61 cgggaagtgt ggcgctttct catagctcac actctggtat ctcggctcgg tgtaggtcgt
      121 tcgctccaag ctgggctgta agcaagaacc ccccgttcag cccgactgct gcgccttatc
      181 cggtaactgt tcacttgagt ccaacccgga aaagcacgca aaacgccact ggcagcagcc
      241 attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg ctcttgaagt
      301 gtgcgccaaa gtccggctac actggaagga cagatttggt tgctgtgctc tgcgaaagcc
      361 agttaccacg gttaagcagt tcccaactga cttaaccttc gatcaaacca cctccccagg
      421 tggtttttcg tttacagggc aaaagattac gcgcagaaaa aaaggatctc aagaagatcc
      481 tttgatcttt tctactgaac cgcgatcccc gtcagttcag aggaggacga tggtgccacc
      541 gttccaccct gaacatcccg gatatagttt gcctgacatg cgaccagaag aaagcctgaa
      601 aaaatcaata taaaaagaaa aacaaccttt acctttttca tataccttcc ctgtatttaa
      661 aaaccccggt attcatccct gaacaccgga agtctgtatt tcaaatcatc cttgataact
      721 tttgcttacc agttaccatc ccggtaaaca aaggatacaa agtaatcaaa tttactaata
      781 tcgattacat ttttaaagtg tggctgtaat atcgaaaccc atgatttttc aacatcaaac
      841 caaccattat taatattatc ctttaaaggc atccccagac tttcaatgac agaaccatca
      901 tcaccgaagt cttttgagta ttcaccacct ttaaactctt cggttttctt atcaaaccaa
      961 tgaatatgta atttaagccc catatttacc tcttaaagat attttttaat gtttcgtttt
     1021 ggatcggccc tttaacctgc ttacccgttt ttggatcgaa tgccccgagg tgttcgccat
     1081 cactggcgcg gtacccttca agctcaccgt gctgggaatc ccattcataa attttacgct
     1141 ttttatcacc ataccatcgg gcccgcttac caccaccacc ttgtttaggg gttttaggtt
     1201 taccctcttt caactctccc aaccccttaa tatcttcagt cttgggatca ggaaaataat
     1261 catggccgta atctttagtt cccttacgag gttttttctt ttcctcattt aatttatttt
     1321 ctgcatcctt cgcttttttc tccttttgtt tccggctctc catcgccccg gaaagcgcag
     1381 ccttatccct ggataaggat tcattcattt cgtttaaaga tgtctgggca gcttttaatt
     1441 tctcattctg attctgaata tccttatcaa gatagcgaac ctgatcctgc cacccggagc
     1501 catattcatg aggcgggtta taagtcacaa aatcatcttt atttgcctct gcctctttta
     1561 cctttttatc ggcttcctga acagcagccc ttgcaccggg gatcgccttc tctgtcgatg
     1621 caatgcgact gtttaaggtc gcaatatttt tatcttcagc atccagctca gctttcgctt
     1681 tatcatactc tcttttcagc ccctcttccg ggtgagcagc gtcccatgcc tgctggcgac
     1741 gcttttcttc ctccagacgc tgttttacct gtgccggagt aggggtctga cgctcagtgg
     1801 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1861 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1921 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     1981 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2041 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     2101 gggcactgtt gcaaagttag cgatgaggca gccttttgtc ttattcaaag gccttacatt
     2161 tcaaaaactc tgcttaccag gcgcatttcg cccaggggat caccataata aaatgctgag
     2221 gcctggcctt tgcgtagtgc acgcatcacc tcaatacctt tgatggtggc gtaagccgtc
     2281 ttcatggatt taaatcccag cgtggcgccg attatccgtt tcagtttgcc atgatcgcat
     2341 tcaatcacgt tgttccggta cttaatctgt cggtgttcaa cgtcagacgg gcaccggcct
     2401 tcgcgtttga gcagagcaag cgcgcgacca taggcgggcg ctttatccgt gttgatgaat
     2461 cgcgggatct gccacttctt cacgttgttg aggattttac ccagaaaccg gtatgcagct
     2521 ttgctgttac gacgggagga gagataaaaa tcgacagtgc ggccccggct gtcgacggcc
     2581 cggtacagat acgcccagcg gccattgacc ttcacgtagg tttcatccat gtgccacggg
     2641 caaagatcgg aagggttacg ccagtaccag cgcagccgtt tttccatttc aggcgcataa
     2701 cgctgaaccc agcggtaaat cgtggagtga tcgacattca ctccgcgttc agccagcatc
     2761 tcctgcagct cacggtaact gatgccgtat ttgcagtacc agcgtacggc ccacagaatg
     2821 atgtcacgct gaaaatgccg gcctttgaat gggttcatgt gcagctccat cagcaaaagg
     2881 ggatgataag tttatcacca ccgactattt gcaacagtgc cgtttccaaa aggtcgttga
     2941 tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat
     3001 cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg
     3061 tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg
     3121 cgatcaccgc ttccctcatg atgtttaacg tttgacatga ggggcggcca agggcgccag
     3181 cccttggacg tccccctcga tggaagggtt aggcaacact gcgtgttcgc tcgaatgcct
     3241 ggcgtgtttg aaccatgtac acggctggac catctggggt ggttacggta ccttgcctct
     3301 caaaccccgc tttctcgtag catcggatcg ctcgcaagtt gctcggcgac gggtccgttt
     3361 ggatcttggt gacctcggga tcattgaaca gcaactcaac cagagctcga accagcttgg
     3421 ttcccaagcc tttgcccagt tgtgatgcat tcgccagtga ctggtctatt ccgcgtactc
     3481 ctggatcggt ttcttcttcc caccatccgt ccccgcttcc aagagcaacg tacgactggg
     3541 ctaacccaat cggctctcca ttcagcattg caatgtatgg agtgacggac tcttgcgcta
     3601 aaacgcttgg caagtactgt tcctgtacgt cagcaagtgt cgggcgtggc ttcttctccg
     3661 ccccaccact cgacgatatg agatcgattt agccactcat agagcatcgc aaggtcatgc
     3721 tcagtcatga ggcgcagttg acggaatcgt tgctgttggt cacgatgctg tactttgtga
     3781 tgcctaactt tgtttggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     3841 gcatttatca ggggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     3901 aaacaaatag gggttccgcg cacttttccc cgaaaagtgc cacctgacgt ctaagaaacc
     3961 attattatca tgacattaac ctataaaata ggcgtatcac gaggcccttt cgtcttcaag
     4021 aattttataa accgtggagc gggcaatact gagctgatga gcaatttccg ttgcaccagt
     4081 gcccttctga tgaagcgtca gcacgacgtt cctgtccacg gtacgcctgc ggccaaattt
     4141 gattcctttc agctttgctt cctgtcggcc ctcattcgtg cgctctagga tctccggcgt
     4201 tcagcctgtg ccacagccga caggatggtg accaccattt gcccatatca ccgtcggtaa
     4261 ctgatcccgt cgtcaataaa ccgaaccgct acaccctgag catcaaaact cttttatcag
     4321 tggatcatgt cggcggtgtc gcggccaaga cggtcgagct tcttcaccag aatgacatca
     4381 ccttcctcca ccttcatcct cagcaaatcc agcccttccc gatctgttga actgccggat
     4441 gccttgtcgg taaagatgcg gttagctttt acccctgcat ctttgagcgc tctgatctga
     4501 atatcgaggg actgctgggc tggttgagac ccgcgcataa ccaaaaattc gcataaaaat
     4561 gtaccttaaa tcgaatatca gacacgatgt gtctattatg ccaaaatgac gatttaatgg
     4621 acactcaaac gaagccgttt tactatgtct gataatttat aatatttcga acggttgcag
     4681 ttgtgttaaa aaagccgtca ggccagggag gccgatatgc ccgttgattt tttgaccact
     4741 gagcaggttg agagttatgg caggttcact ggcgaacccg atgaacttca gctggcgcgt
     4801 tattttcatc ttgatgaagc ggataaagaa tttatcggga aaagccgggg tgatcacaat
     4861 cgacttggta ttgccctgca aatcgggtgt gtgcgttttc tgggcacttt tcttactgga
     4921 catgaatcat atttcttccg gcgtccggca tttaccgcca gacagctcgg gattcgtata
     4981 tcaccgttct tgcagaatac ggtcagaggg aaaatacccg ccgtgagcat gcagcgctga
     5041 tacgtcagca ctatcagtat cgtgaatttg cctggccctg gacatttcgc cttacccgtc
     5101 ttttatatac ccggagctgg ataagcaacg aacgtcctgg cctgcttttc gacctggcga
     5161 caggtggctt atgcaacatc gtattattct ccccggagcc accacgctga cccggttgat
     5221 ttcagaggta agggaaaagg cgacgttgcg cctgtggaac aaaactggca ctgataccgt
     5281 cagccgaaca gcgttcacag ctggagatgc tgctgggcca actgattgca gccgcctgtc
     5341 tttactggaa tcactgaaaa aaggccctgt gaccatcagt ggtccggcgt ttaatgaagc
     5401 aattgaacgc tgggaaaact ctgaacgatt ttggcctgca tgctgaaaac ctgagtacac
     5461 tcccggctgt gcgccctgaa aaatctcgca cgttatgctg gtatgacttc ggtgttcaat
     5521 attgccagga tgtcaccgca gaaaaggatg gcggttctgg ttgcctttgt ccttgcatgg
     5581 gaaaacgctg gcgctggatg atgcactgga cgttctggac gccatgctgg ccgttatcat
     5641 ccgtgacgcc agaaagattg ggcagaaaaa acggctccgc tcgctgaagg atctggataa
     5701 atctgcattg gcgctcgcca gcgcatgttc gtacttgctg aaagaagaaa caccggacga
     5761 atcgattcgt gctgaggtgt tcagctacat ccctaggcaa aagctggctg aaatcatcac
     5821 gcttgtccgt gaaattgccc ggccctcaga cgataatttt catgacgaaa tggtggagca
     5881 gtacgggcgc gttcgtcgtt tcctgcccca tctgctgaat accgttaaat tttcatccgc
     5941 acctgccggg gttaccactc tgaatgcctg tgactacctc agccgggagt tcagctcacg
     6001 gcggcagttt ttttgacgac gccaccaacg gaaatcatca gtcagtcatg gaaacggctg
     6061 gtgattaaca aggaaaaaca tatcacccga aggggataca cgctctgctt tctcagtaaa
     6121 ctgcaggata gtctgagacg gagggatgtc tacgttaccg gcagtaaccg gtggggagat
     6181 cctcgtgcaa gattactaca gggtgctgac tggcaggcaa atcggattaa ggtttatcgt
     6241 tctttggggc acccgacaga cccgcaggaa gcaataaaat ctctgggcca tcagctgata
     6301 gtcgttacag acaggttgct gcacgtcttg gcgaaaatga ggctgtcgaa ctcgatgttc
     6361 tggcccgaag ccccggttga caatttctcc cctcgccagt cttgatgagc cggacagtct
     6421 gaaacgactg agcaaaatga tcagtgatct gctcctccgg tggatttaac ggagttgctg
     6481 ctcgaaatta acgcccatac cggattgctg atgagttttt ccatgccagt gaagccagtg
     6541 ccagagttga tgatctgccc gtcagcatca gcgccgtgct gatggctgaa gcctgcaata
     6601 tcggtctgga accactgatc agatcaaatg ttcctgcact gacccgacac cggctgaact
     6661 ggacaaaagc gaactatctg cgggctgaaa ctatcaccag cgctaatgcc agactggttg
     6721 attttcagca acgctgccac tggcacagat atggggtgga ggagaagtgg catctgcaga
     6781 tggaatgcgc tttgttacgc cagtcagaac aatcaatgcc ggaccgaacc gcaaaatact
     6841 ttggtaataa cagagggatc acctggtaca actttgtgtc cgatcagtat tccggctttc
     6901 atggcatcgt tataccgggg acgctgaggg actctatctt tgtgctggaa ggccttctgg
     6961 aacaggagac cgggctgaat ccaaccgaaa ttatgaccga tacggcaggt gccagcgatc
     7021 ttgtctttgc cttttctggc tgctgggata ccagttttct ccacgcctgg ctgatgccgg
     7081 tgcttcggtt ttctggcgaa tggaccatga tgccgactat ggcgtgctga atgatattgc
     7141 cagaggggca atcagatccc cgaaaaatag tccttcagtg ggacgaaatg atccggaccg
     7201 caggctccct gaagctgggg caaagtacag gcctcagtgc tggtccgttc attgctgaaa
     7261 agtgaacgtc cctccggact ggactcaggc aatcattgaa gtggggcgca tcaacaaaac
     7321 gctgtatctg cttaattata ttgatgatga agattaccgc cggcgcattc tgacccagct
     7381 taatcgggga gaaagccgtc atgcagttgc cagagccatc tgtcacggtc aaaaaggtga
     7441 gataagaaaa cgatataccg acggtcagga agatcagttg ggagctctgg ggctggtcac
     7501 tacgccgtcg tgttatggaa cactatttat atgcaggcag ctctggatca tctccggggc
     7561 gcagggtgaa acactgaatg atgaagatat cgcacgcctc tccccgcttt gccacggaca
     7621 tatcaatatg ctcggccatt attccttcac gctggcagaa ctggtgacca aaggtcatct
     7681 gagaccatta aaagaggcgt cagaggcaga aaacgttgct taacgtgagt tttcgttcca
     7741 ctgagcgtca gacccctttt gatcccaatt tttccaccca gaaagctgac acaacacgaa
     7801 tgatggtgcc ttttttccgc agttctggga tatgggcgca aaggataaag cgaccggaaa
     7861 atcacggagt gatgtgcagc aacgcattca gcagtttcac agtcaggaat ttttaaactc
     7921 actccaaggc acaacacaat ttgcgggtac cgattaccgc agcaaagacc tcacaccgaa
     7981 aaatcaaggc tactggctga caccatttca gctgtttatc tcgatggcta tgaaggcaga
     8041 cagtaagtgg atttaccata acgcgtcatt acgcgcaccg ctcagacggg ctcagggcgt
     8101 tccggcggct acgggataaa acaggcaaca accccccgga aaactgccgg aaacgcgccc
     8161 gataaatttt atcctaatgc atgcctatgc acgcaactgc catggcatat gttttgacgt
     8221 aaaatggatg ccgtaagccc ttttttaccg actttctgct tcatcagcct gactgacacg
     8281 accatattct ttcagaccgc cccctgctaa acttccttca tattgccgca ttaacccggg
     8341 aggctccgca atgagcaaca ctacacacta cgaaaacgcc aactttcttc gggaactggc
     8401 tgaagcctgc cccgcatact gccggaaggt ggcccggaca aagctgcgct gttgcagcga
     8461 ctggctaacg aagaactggc ccaggcagag tatgaagatc aggttcgcgc caaagtgacc
     8521 gccgcccgtg ctgacacgcg tccgggtatg accacggaac aactccgcca gcgcctgcat
     8581 ggccgttatc aggaactgcg cgatgccgta tgacgtggaa tggaagcagg gagcatcgag
     8641 gatgtaacga cgctgtttga ctatatcgcg gaaaattcat ccctgtggga tgcgcagaac
     8701 gttaccgacc gggttctcgc tgcggcggac aaactggccg acttcccgcg actctatgaa
     8761 gtggactcac gctatggcga ggacgttcgc cggatcagtc tgatgggaca gcatgtcctc
     8821 tatgatgttg atgaccagac cagaaaagtc cgggttctgg ccgtggtcgg ccagcgccag
     8881 aatccccata ttgttctcta ataaccggtg acggggacgc aattcattgc gtggccgtcg
     8941 ccggttggac aagcaccagc gcgtcagcgt tttccccacg gccaccaccg gcgcggtgtt
     9001 ccggccgggg tctctgctgt ctccggtttg tgtccagcag tctcatggac tgcatttcct
     9061 gccgtagccc ggccacctcc tccctgagtg cttttatctc cgcgatcaac gcgtcggagg
     9121 ccattttttc agtctcaccc gacagtctca actcgtcccg ctcgtgagtc tcatcctgtc
     9181 ttaactcacc gtacacccgg ataagctcgc tggtatccac cacccggccg ccatcagctt
     9241 ccgtacggta tgatacgcgg cctttcgcca tatcccgata cagggaactt cgggctttac
     9301 ccgtcagttc cgtcgcctgt ctcaccgtca cccacgccat tgtctcaccc cgctattttt
     9361 gagacagtat acctgacagg cgcacaccat gcccggaagg aaagaatcat gacaaaacag
     9421 ggaaaaaaca gccctcaata tggctaaatt tattaaggag cagtcgctgc tattgctcga
     9481 taaactgaat gagctggata tggatacccc ggccgacatg tgcgagcggc tacacgagga
     9541 tgcggaaaca ctctggcagg tgatgcggga taagctcggg gaggaataac agcaccggca
     9601 tggccggtgc tggcatttat tttttgccga acggcaccac ggcgggcagg tttgtcctcc
     9661 ggcagttcag gtaccggcac gttaaaatag cggctgttat attcccgcat cagctccgct
     9721 ttcagcttct cgttcaccgc gctttccccg tgccgctcac tcaccacgtc aatcatcgcg
     9781 ttaatctgct ccggtgagca cttcccggca tcaaccacct gcacaccgtt ctcgatggcg
     9841 aaccgctcca ccttgcgata gaggtaatca cacagcttca ttcctgtggg tttgttccgt
     9901 tcccattgcg gatttttacc agtgaaaaag tacgtggctt tttcgtcgct gttgagccgc
     9961 tcgtcataca tcccgatgga tttcatcatc gccagatgct gggcccggtc gacaaaacag
    10021 aaaacatacg ccccggtctg ccaggcgagc accaccatat cttccagatg ctcgctggtc
    10081 ggcatgatgc agcgctcgcc gttccggtcc tccgcgtctt caataaatac catgtacagc
    10141 gccataatcc ctcactccgt tgtcataaat ttaaagcacg caaacaaccg ttcacgatcc
    10201 agctctgccg ttttgcgggc ctcgccggtc tgtgggttca cgatttcatc caccgacagg
    10261 attgcccgct gcaccctgac ctgcgtcgcc cggtccgggc agatgtacca gatttcatcg
    10321 atgccgttag ctttgcggtt gaacagatac ccggacacca cttcctgata ccggcgacgg
    10381 ctcttgacgg tgcgctccac ctcaaacgcc accgttttac cgtccagctc cgccagggcg
    10441 tccggcactt tcatgccttt aagactcaga cggtgcagaa tgcgccagcc ggtggcccct
    10501 ctggcttcca tatcaaggcg cagggattgc acggccagcc ggtgcggcag atgtgctgcc
    10561 gacacccttg acggctgaaa cggaataaca tccgccagcg gttcgtcctc gtcgaacgag
    10621 aacgccacgc catgcggcgt cagcccccac acgctgacct gacggccgtc caccggatat
    10681 ttggtccgga ccagcagccc tttcgccacc aggctgtcga ggaagcggta gaccggctgc
    10741 accgccgcaa tccccagccg ccgcgcaacc agctccgccg tggaacacag ctcttcccgc
    10801 aaccagcgca gggtcagccg ggttttctcc gcgttccgcg cctgtcgttt cgcgtggctg
    10861 acgttgtaca gctccagcgc catcagtaaa tccccgcaaa atcatcatcc gctttcggcc
    10921 cgccgccgtc ggccgggggc cggaactccg ccaggggatc ggttttcgcc agcaccggga
    10981 aacgctgcaa cgggatttcc cggcgctcaa cccgcagcgg actgctgaac gccatccggg
    11041 ccacacccag cccggtcatg accgcgaacc cgtccggcag gtgttgcagg gtgttcacgt
    11101 caaacagcgg ccgcgcctcc ttctggatga agctgtcccc gctgatatgc tccacgttcc
    11161 ccgcctcccg gctggcacgg cggcgctcca cgtccaccct gatttccccg gtctgccccg
    11221 ccgcccactg cgcggactcc aggctggccg cccggtaaaa ccagcggatc ggcgtgttat
    11281 ccagtaccgt ggttttcaca aattcagcgg gcagatcctg cccgcactgc ccgaaatcgc
    11341 ccagcgactg gtgcgcgagc aacagattgc agtcccggct gcgcagcgtc cccagtgcgt
    11401 tcagtacata cttcgacagc agatatttaa tttcatcgag catcacgctg acgtgtggcc
    11461 actcccggaa ctcgtcccgc gcaatgatga tttgcgcgca gcgggcaaac agcatttttt
    11521 gcacacggat caccgcctcg tcgtccatcg agccgataac gtacaggcag ccgccgccgt
    11581 tgataatccc cgccacatcg atgccactgt ccgtctgcag gacgctcagg tcagcgacgt
    11641 tttccagcct agtgatcaac cccttcacgt cctttttcag ggcctccggc agcgcgtacg
    11701 ccgccgccag cacctgctga atgtttgccc cctgcggaaa ctgctccgca atcagcttcg
    11761 ccgctttctg ctcgctgatg cggtagtggt ccgccacgtc gccggtctcg cccaggttaa
    11821 gcccggcaat cagcaggttt ttcagcgcgt actggtcgag atcgcggaac agattcagct
    11881 gcgccggttt ccccggccgc agatccagca gggtgaagtc cggacagtgc gcccggaaga
    11941 cgtgcggcgc ccatgcgtcc cctttggggt cgaagtacac caccgcgtcc ccgaaatcgc
    12001 gtacgcagcg aatgagcgcg ttggtggcca tcacgctctt gccgctcccg ggcaggccga
    12061 ggatctgaat gttggtcttg cgccaggtat cacgcggaac cagcaccggc ttgccctgct
    12121 catccttacc cagaaacacc gcctgctcct gccgggcctg ctccgaggaa aaatcggcca
    12181 gcgcattaac cggtaccggc tccccgaaat gcaggtggcg cacgtcggtc atctctcctg
    12241 tccgctggct gctgcgccgg ctctcctgat tcagtttcgc gttcaccgcc tcgtcagcag
    12301 ccgcaaaatg accgtcaagc agcaggaacg ccagcagccg cgccaacaca atcccgacag
    12361 ccagcccgcc cgcgcaccag gcaatcccga acaggttagc cggctgcagc aggtaataat
    12421 ctgcgtttgc gtagaggtcc ggacgcagga cggtttctcc ccagctctcg ataaacacgg
    12481 cgacaaccag cccccccaga agggccagac cggccgcgcc ggtcagccag tacgcgtagt
    12541 tccagacatg tcgacgtgcc ctgaccatcc gcccgggcga atccagcgag gccagcaccg
    12601 aaaatggcgc tggccgttta ttatcgcgct tatagcgatc ggcatactcc cgtcgctcct
    12661 tatgactcag ccccacaaaa cgcatccgtc cggcccaaag ttcagccaga ccgaaaaaca
    12721 ggccgataac taggaaagcc ccggccagac cgtaatactc ctggtacagc cagctgaccc
    12781 acggataaat ctgccgggcc agctccggaa tgtgccactt ccaggcaagc gaatccgtat
    12841 taaacatgtt ctctctcctt cagacgatgg tttgccggcg tgatgtgcag cccccgctgc
    12901 acaagccgcg gacatgcccc aaggcatggc ggcggcggtc acgtccgtgg tgattgcgtt
    12961 cagctttcac cacggaggcc agcctctttc cgccccatgc ggaaaaggcg cagccccgga
    13021 ggaacaacga gcacgcccca gcgtgtgaga ggaaaaggcg gcaccccagt gcggtctttt
    13081 ccggtgttcc ggcccccgtc agagttcgcc atagcgagcg gctgaccacc cgcaaaatca
    13141 ggagcagctt tgctgcttcg ccaggtgcgt ccagcaccgt ggatttttgt gggtggtcag
    13201 gcgtgagcct gacgggggcc gggtgaattt ctggcgtggc cggcgtccag ccggacggag
    13261 ccagaaagag ggggagagac cgtgcgtcgg tggcgtccag ccagcgtaag caggggatat
    13321 ccccctgagg tcagcccacg ccctcgcggg gatgacctgt atttctccgt aaggagaaaa
    13381 aagcaacaga gataggttaa cgtaaaatgt tcagcgaaaa accgatatga ttcacctatc
    13441 ctggtaattt attatcaggg aatataaaga cttaatcagg ggacaatatg ccacatcagc
    13501 gcgtccggtg tccgtcttgt aggtgccggg gaaattgcgc ctcctcgctc actcggtcgc
    13561 tacgctccgg ccgtgagact gcggcgggcg ctgcggacac atgcaaagtt acccacagat
    13621 tccgtgaata agcaggggac taacatgtga ggcaaaacgg caaggccacg caggtggcgt
    13681 ttttttccat aggctccgcc ctcctgactg agttcacata aacagacgct tttttcggtg
    13741 catctgtggg gagccggtgg ctcaaccatg aatcagtcag gagtggcgaa acccgacagg
    13801 acttaaagat catcaccgtt ccgatgaatc gctccctc
//
