LOCUS       NZ_CP011979            20222 bp    DNA     circular CON 25-DEC-2023
DEFINITION  Klebsiella pneumoniae DMC1097 plasmid pDMC1097-20.222kb, complete
            sequence.
ACCESSION   NZ_CP011979
VERSION     NZ_CP011979.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02141981
            Assembly: GCF_000417225.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae DMC1097
  ORGANISM  Klebsiella pneumoniae DMC1097
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 20222)
  AUTHORS   Wright,M.S., Perez,F., Brinkac,L., Jacobs,M.R., Kaye,K., Cober,E.,
            van Duin,D., Marshall,S.H., Hujer,A.M., Rudin,S.D., Hujer,K.M.,
            Bonomo,R.A. and Adams,M.D.
  TITLE     Population structure of KPC-producing Klebsiella pneumoniae isolates
            from midwestern U.S. hospitals
  JOURNAL   Antimicrob Agents Chemother 58 (8), 4961-4965 (2014)
   PUBMED   24913165
REFERENCE   2  (bases 1 to 20222)
  AUTHORS   Adams,M., Sanka,R., Wright,M., Brinkac,L., Perez,F., Jacobs,M.,
            Kaye,K., Cober,E., Duin,D., Marshall,S., Hujer,A., Rudin,S.,
            Hujer,K. and Bonomo,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2015) JCVI, 9704 Medical Center Drive, Rockville,
            MD 20850, USA
REFERENCE   3  (bases 1 to 20222)
  AUTHORS   Brinkac,L.M., Durkin,A.S., Haft,D.H., McCorrison,J., Wright,M.,
            Duin,D.V., Kaye,K.S., Marshall,S., Rudin,S., Hujer,A., Perez,F.,
            Bonomo,R.A. and Adams,M.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2013) The J. Craig Venter Institute, 9704 Medical
            Center Dr., Rockville, MD 20850, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Assembly Name                     :: Klebsiella pneumoniae DMC1097
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10.02x
            Sequencing Technology             :: PacBio RS
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/25/2023 03:12:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,692
            CDSs (total)                      :: 5,568
            Genes (coding)                    :: 5,449
            CDSs (with protein)               :: 5,449
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 72 of 119
            Pseudo Genes (internal stop)      :: 13 of 119
            Pseudo Genes (multiple problems)  :: 19 of 119
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP011979.1.
            This strain is part of the Klebsiella pneumoniae Genome Sequencing
            Center Project as part of the Genome Sequencing Centers for
            Infectious Diseases contract.
            http://gsc.jcvi.org/projects/gsc/klebsiella_pneumoniae/index.php.
            Source DNA and/or bacteria are available from the laboratory of Dr.
            Robert Bonomo, Cleveland VA Medical Center, 19701 East Boulevard,
            Cleveland, OH 44106, USA.  This project has been funded in whole or
            in part with federal funds from the National Institute of Allergy
            and Infectious Diseases, National Institutes of Health, Department
            of Health and Human Services through the Genomic Sequencing Centers
            for Infectious Diseases under contract number HHSN272200900007C.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20222
                     /organism="Klebsiella pneumoniae DMC1097"
                     /mol_type="genomic DNA"
                     /strain="DMC1097"
                     /db_xref="taxon:1284798"
                     /plasmid="pDMC1097-20.222kb"
     gene            complement(1433..1582)
                     /locus_tag="H218_RS28695"
                     /old_locus_tag="H218_28755"
     CDS             complement(1433..1582)
                     /locus_tag="H218_RS28695"
                     /old_locus_tag="H218_28755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(1667..1924)
                     /locus_tag="H218_RS28700"
                     /old_locus_tag="H218_28760"
     CDS             complement(1667..1924)
                     /locus_tag="H218_RS28700"
                     /old_locus_tag="H218_28760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(1934..3619)
                     /locus_tag="H218_RS28705"
                     /old_locus_tag="H218_28765"
     CDS             complement(1934..3619)
                     /locus_tag="H218_RS28705"
                     /old_locus_tag="H218_28765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(3756..6761)
                     /locus_tag="H218_RS28710"
                     /old_locus_tag="H218_28770"
     CDS             complement(3756..6761)
                     /locus_tag="H218_RS28710"
                     /old_locus_tag="H218_28770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143775.1"
                     /translation="MPVDFLTTEQVESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHDEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISQSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            6923..7480
                     /locus_tag="H218_RS28715"
                     /old_locus_tag="H218_28775"
     CDS             6923..7480
                     /locus_tag="H218_RS28715"
                     /old_locus_tag="H218_28775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            7663..>8112
                     /locus_tag="H218_RS28720"
                     /old_locus_tag="H218_28780"
                     /pseudo=""
     CDS             7663..>8112
                     /locus_tag="H218_RS28720"
                     /old_locus_tag="H218_28780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /name="class A beta-lactamase"
     gene            8176..8880
                     /locus_tag="H218_RS28725"
                     /old_locus_tag="H218_28785"
     CDS             8176..8880
                     /locus_tag="H218_RS28725"
                     /old_locus_tag="H218_28785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            <8935..9105
                     /locus_tag="H218_RS30435"
                     /pseudo=""
     CDS             <8935..9105
                     /locus_tag="H218_RS30435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /name="serine hydrolase"
     gene            complement(9557..>9727)
                     /locus_tag="H218_RS30445"
                     /pseudo=""
     CDS             complement(9557..>9727)
                     /locus_tag="H218_RS30445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /name="serine hydrolase"
     gene            complement(9782..10486)
                     /locus_tag="H218_RS28730"
                     /old_locus_tag="H218_28790"
     CDS             complement(9782..10486)
                     /locus_tag="H218_RS28730"
                     /old_locus_tag="H218_28790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(<10550..10999)
                     /locus_tag="H218_RS28735"
                     /old_locus_tag="H218_28795"
                     /pseudo=""
     CDS             complement(<10550..10999)
                     /locus_tag="H218_RS28735"
                     /old_locus_tag="H218_28795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /name="class A beta-lactamase"
     gene            complement(11182..11739)
                     /locus_tag="H218_RS28740"
                     /old_locus_tag="H218_28800"
     CDS             complement(11182..11739)
                     /locus_tag="H218_RS28740"
                     /old_locus_tag="H218_28800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            11901..14906
                     /locus_tag="H218_RS28745"
                     /old_locus_tag="H218_28805"
     CDS             11901..14906
                     /locus_tag="H218_RS28745"
                     /old_locus_tag="H218_28805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143775.1"
                     /translation="MPVDFLTTEQVESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHDEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISQSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            14983..15402
                     /locus_tag="H218_RS28750"
                     /old_locus_tag="H218_28810"
     CDS             14983..15402
                     /locus_tag="H218_RS28750"
                     /old_locus_tag="H218_28810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            15708..15971
                     /locus_tag="H218_RS28755"
                     /old_locus_tag="H218_28815"
     CDS             15708..15971
                     /locus_tag="H218_RS28755"
                     /old_locus_tag="H218_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            15961..16260
                     /locus_tag="H218_RS28760"
                     /old_locus_tag="H218_28820"
     CDS             15961..16260
                     /locus_tag="H218_RS28760"
                     /old_locus_tag="H218_28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(16322..16699)
                     /locus_tag="H218_RS28765"
                     /old_locus_tag="H218_28825"
     CDS             complement(16322..16699)
                     /locus_tag="H218_RS28765"
                     /old_locus_tag="H218_28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            16768..16947
                     /locus_tag="H218_RS28770"
                     /old_locus_tag="H218_28830"
     CDS             16768..16947
                     /locus_tag="H218_RS28770"
                     /old_locus_tag="H218_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(16975..17505)
                     /locus_tag="H218_RS28775"
                     /old_locus_tag="H218_28835"
     CDS             complement(16975..17505)
                     /locus_tag="H218_RS28775"
                     /old_locus_tag="H218_28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(17512..18243)
                     /locus_tag="H218_RS28780"
                     /old_locus_tag="H218_28840"
     CDS             complement(17512..18243)
                     /locus_tag="H218_RS28780"
                     /old_locus_tag="H218_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(18243..20207)
                     /locus_tag="H218_RS28785"
                     /old_locus_tag="H218_28845"
     CDS             complement(18243..20207)
                     /locus_tag="H218_RS28785"
                     /old_locus_tag="H218_28845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     ARG             complement(10524..10999)
                     /name="TEM-10"
                     /gene="TEM-10"
                     /gene_name="TEM beta-lactamase"
                     /tool="rgi"
                     /drug_class="monobactam; cephalosporin; penam; penem"
                     /identity="96.77"
                     /coverage="55.24"
     ARG             7664..8139
                     /name="TEM-10"
                     /gene="TEM-10"
                     /gene_name="TEM beta-lactamase"
                     /tool="rgi"
                     /drug_class="monobactam; cephalosporin; penam; penem"
                     /identity="96.77"
                     /coverage="55.24"
     BGC             1..8619
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          752..1330
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(17516..18243)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.35e-150"
ORIGIN
        1 gacgatggtt tgccggcgtg atgtgcagcc cccgctgcac aagccgcgga catgccccaa
       61 ggcatggcgg cggcggtcac gtccgtggtg attgcgttca gctttcacca cggaggccag
      121 cctctttccg ccccatgcgg aaaaggcgca gccccggagg aacaacgagc acgccccagc
      181 gtgtgagagg aaaaggcggc accccagtgc ggtcttttcc ggtgttccgg cccccgtcag
      241 agttcgccat agcgagcggc tgaccacccg caaaatcagg agcagctttg ctgcttcgcc
      301 aggtgcgtcc agcaccgtgg atttttgtgg gtggtcaggc gtggccggcg tccagccgga
      361 cggagccaga aagaggggga gagaccgtgc gtcggtggcg tccagccagc gtaagcaggg
      421 gatatccccc tgaggtcagc cacgccctcg cggggatgac ctgtatttct ccgtaaggag
      481 aaaaaagcaa cagagatagg ttaacgtaaa atgttcagcg aaaaaccgat atgattcacc
      541 tatcctggta atttattatc agggaatata aagacttaat caggggacaa tatgccacat
      601 cagcgcgtcc ggtgtccgtc ttgtaggtgc cggggaaatt gcgcctcctc gctcactcgg
      661 tcgctacgct ccggccgtga gactgcggcg ggcgctgcgg acacatgcaa agttacccac
      721 agattccgtg aataagcagg ggactaacat gtgaggcaaa acggcaaggc cacgcaggtg
      781 gcgtttttcc ataggctccg ccctcctgac tgagttcaca taaacagacg ctttttcggt
      841 gcatctgtgg gagccggtgg ctcaaccatg aatcagtcag gagtggcgaa acccgacagg
      901 acttaaagat catcaccgtt tccgatgaat cgctccctct tgcgctctcc tgttccaacc
      961 ctgccgtttc ccggatacct gttccgcctt tctcccttgc gggaagtgtg gcgctttctc
     1021 atagctcaca ctctggtatc tcggctcggt gtaggtcgtt cgctccaagc tgggctgtaa
     1081 gcaagaaccc cccgttcagc ccgactgctg cgccttatcc ggtaactgtt cacttgagtc
     1141 caacccggaa aagcacgcaa aacgccactg gcagcagcca ttggtaactg ggagttcgca
     1201 gaggatttgt ttagctaaac acgcggttgc tcttgaagtg tgcgccaaag tccggctaca
     1261 ctggaaggac agatttggtt gctgtgctct gcgaaagcca gttaccacgg ttaagcagtt
     1321 ccccaactga cttaaccttc gatcaaacca cctccccagg tggttttttc gtttacaggg
     1381 caaaagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctactgaa
     1441 ccgcgatccc cgtcagttca gaggaggacg atggtgccac cgttccaccc tgaacatccc
     1501 ggatatagtt tgcctgacat gcgaccagaa gaaagcctga aaaaatcaat ataaaaagaa
     1561 aaacaacctt tacctttttc atataccttc cctgtattta aaaaccccgg tattcatccc
     1621 tgaacaccgg aagtctgtat ttcaaatcat ccttgataac ttttgcttac cagttaccat
     1681 cccggtaaac aaaggataca aagtaatcaa atttactaat atcgattaca tttttaaagt
     1741 gtggctgtaa tatcgaaacc catgattttt caacatcaaa ccaaccatta ttaatattat
     1801 cctttaaagg catccccaga ctttcaatga cagaaccatc atcaccgaag tcttttgagt
     1861 attcaccacc tttaaactct tcggttttct tatcaaacca atgaatatgt aatttaagcc
     1921 ccatatttac ctcttaaaga tattttttaa tgtttcgttt tggatccggc cctttaacct
     1981 gcttacccgt ttttggatcg aatgccccga ggtgttcgcc atcactggcg cggtaccctt
     2041 caagctcacc gtgctgggaa tcccattcat aaattttacg ctttttatca ccataccatc
     2101 gggcccgctt accaccacca ccttgtttag gggttttagg tttaccctct ttcaactctc
     2161 ccaacccctt aatatcttca gtcttgggat caggaaaata atcatggccg taatctttag
     2221 ttcccttacg aggttttttc ttttcctcat ttaatttatt ttctgcatcc ttcgcttttt
     2281 tctccttttg tttccggctc tccatcgccc cggaaagcgc agccttatcc ctggataagg
     2341 attcattcat ttcgtttaaa gatgtctggg cagcttttaa tttctcattc tgattctgaa
     2401 tatccttatc aagatagcga acctgatcct gccacccgga gccatattca tgaggcgggt
     2461 tataagtcac aaaatcatct ttatttgcct ctgcctcttt taccttttta tcggcttcct
     2521 gaacagcagc ccttgcaccg gggatcgcct tctctgtcga tgcaatgcga ctgtttaagg
     2581 tcgcaatatt tttatcttca gcatccagct cagctttcgc tttatcatac tctcttttca
     2641 gcccctcttc cgggtgagca gcgtcccatg cctgctggcg acgcttttct tcctccagac
     2701 gctgttttac ctgtgccgga gtaagaacat ccgtcaccga cacatagacc ggtttctgtc
     2761 cgctctcttt cgggaaacga ataacggctt cacgggagtt accaccggcc gtaaaaccag
     2821 ccggacgtga atcacctttt tcagcaataa tgccttttgg cggggctttc gcggccggaa
     2881 cacctttagg tacgctcacc tgcagagacg ggagacccgg aatcgacaca ctgaataccc
     2941 ccggacgttt tgtcggtttc gcatccacca caggaacact cattggccgg ccggtgacaa
     3001 ccgcaatatg ctgccgctcg tccttcacaa tatccgccac acgtttagtg acgctgaccg
     3061 tcgcctggtc cagcggcagg gagctgaccg gcgtctccgt taccgtgtct gccggcaatg
     3121 acgtcacgat ttttgacatc attttcggat catcttttgc tatctcagaa ggcagcactc
     3181 cgtaaatcgc aatcccccac agaccaaact taaacggacc tttcagggca gccagcacat
     3241 cagcaacagc ggccgacaac gcatcgccgg aaacggataa agccagtcct tcagcccccg
     3301 gagtggccaa ggcaggcaga ccgaaggcca tcgcggtggc cgaagactgt ccgccaccag
     3361 agctcccgct atgattgccg ctgttaccgc cattaccacc gggtttactc ccgccgttac
     3421 caaactcccc cgggttatag ttatgaatat ccccgttagg tgtatgcgtc gtaccccagc
     3481 ccggcccgct gtttggatcc gtaccaccac ctgatgaagg gccaccttta cctgatgtac
     3541 cgttcacatt cccactggcc tgaccaccat tgtgtcccag acctgaatta cccggacctc
     3601 gtccgtctcc gccactcata tacttttcct ctttgacttt taaaacaata aactaaaata
     3661 aaatactgta catataacca ctggttttat atacagtata aaaattacgc tgccttgttt
     3721 ttggggtctg acgctcagtg gaacgaaaac tcacgttaag caacgttttc tgcctctgac
     3781 gcctctttta atggtctcag atgacctttg gtcaccagtt ctgccagcgt gaaggaataa
     3841 tggccgagca tattgatatg tccgtggcaa agcggggaga ggcgtgcgat atcttcatca
     3901 ttcagtgttt caccctgcgc ccggagatga tccagagctg cctgcatata aatagtgttc
     3961 cataacacga cggcgttagt gaccagcccc agagctccca actgatcttc ctgaccgtcg
     4021 gtatatcgtt ttcttatctc acctttttga ccgtgacaga tggctctggc aactgcatga
     4081 cggctttctc cccgattaag ctgggtcaga atgcgccggc ggtaatcttc atcatcaata
     4141 taattaagca gatacagcgt tttgttgatg cgccccactt caatgattgc ctgagtcagt
     4201 ccggagggac gttcactttt cagcaatgaa cggaccagca ctgaggcctg tactttgccc
     4261 agcttcaggg agcctgcggt ccggatcatt tcgtcccact gaaggactat ttttcgggga
     4321 tctgattgcc ctctggcaat atcattcagc acgccatagt cggcatcatg gtccattcgc
     4381 cagaaaaccg aagcaccggc atcagccagg cgtggagaaa actggtatcc cagcagccag
     4441 aaaaggccaa agacaagatc gctggcacct gccgtatcgg tcataatttc ggttggattc
     4501 agcccggtct cctgttccag aaggccttcc agcacaaaga tagagtccct cagcgtcccc
     4561 ggtataacga tgccatgaaa gccggaatac tgatcggaca caaagttgta ccaggtgatc
     4621 cctctgttat taccaaagta tttgcggttc ggtccggcat tgattgttct gactggcgta
     4681 acaaagcgca ttccatctgc agatgccact tctcctccac cccatatctg tgccagtggc
     4741 agcgttgcct gaaaatcaac cagtctggca ttagcgctgg tgatagtttc agcccgcaga
     4801 tagttcgctt ttgtccagtt cagccggtgt cgggtcagtg caggaacatt tgatctgatc
     4861 agtggttcca gaccgatatt gcaggcttca gccatcagca cggcgctgat gctgacgggc
     4921 agatcatcaa ctctggcact ggcttcactg gcatggaaaa actcatcagc aaatccggta
     4981 tgggcgttaa tttcgagcag caactccgtt aaatccaccg gagggagcag atcactgatc
     5041 attttgctca gtcgtttcag actgtccggc tcatcaagac tggcgagggg agaaattgtc
     5101 aaccggggct tcgggccaga aacatcgagt tcgacagcct cattttcgcc aagacgtgca
     5161 gcaacctgtc tgtaacgact atcaagctga tggcccagag attttattgc ttcctgcggg
     5221 tctgtcgggt gccccaaaga acgataaacc ttaatccggt ttgcctgcca gtcagcaccc
     5281 tgtagtaatc ttgcacgagg atctccccac cggttactgc cggtaacgta gacatccctc
     5341 cgtctcagac tatcctgcag tttactgaga aagcagagcg tgtatcccct tcgggtgata
     5401 tgtttttcct tgttaatcac cagccgtttc catgactgac tgatgatttc cgttggtgcg
     5461 tcgtcaaaaa actgccgccg tgagctgaac tcccggctga ggtagtcaca ggcattcaga
     5521 gtggtaaccc cggcaggtgc ggatgaaaat ttaacggtat tcagcagatg gggcaggaaa
     5581 cgacgaacgc gcccgtactg ctccaccatt tcgtcatgaa aattatcgtc tgagggccgg
     5641 gcaatttcac ggacaagcgt gatgatttca gccagctttt gcctagggat gtagctgaac
     5701 acctcagcac gaatcgattc gtccggtgtt tcttctttca gcaagtacga acatgcgctg
     5761 gcgagcgcca atgcagattt atccagatcc ttcagcgagc ggagccgttt tttctgccca
     5821 atctttctgg cgtcacggat gataacggcc agcatggcgt ccagaacgtc cagtgcatca
     5881 tccagcgcca gcgtttccca tgcaaggaca aaggcaacca gaaccgccat ccttttctgc
     5941 ggtgacatcc tggcaatatt gaacaccgaa gtcataccag cataacgtgc gagatttttc
     6001 aggcgcacag ccgggagtgt actcaggttt tcagcatgca ggccaaaatc gttcagagtt
     6061 ttccagcgtt caattgcttc attaaacgcc ggaccactga tggtcacagg gccttttttc
     6121 agtgattcca gtaaagacag gcggctgcaa tcagttggcc ccagcagcat ctccagctgt
     6181 gaacgctgtt cggctgacgg tatcagtgcc agtttgttcc acaggcgcaa cgtcgccttt
     6241 tcccttacct ctgaaatcaa ccgggtcagc gtggtggctc cggggagaat aatacgatgt
     6301 tgcataagcc accctgtcgc caggtcgaaa agcaggccag gacgttcgtt gcttatccag
     6361 ctccgggtat ataaaagacg ggtaaggcga aatgtccagg gccaggcaaa ttcacgatac
     6421 tgatagtgct gacgtatcag cgctgcatgc tcacggcggg tattttccct ctgaccgtat
     6481 tctgcaagaa cggtgatatc acgaatcccg agctgtctgg cggtaaaatg ccggacgccg
     6541 gaaggaatat gattcatgtc agtaagaaaa gtgcccagaa aacgcacaca cccgatttgc
     6601 agggcaatac caagtcgatt gtgatcaccc cggcttttcc cgataaattc tttatccgct
     6661 tcatcaagat gaaaataacg cgccagctga agttcatcgg gttcgccagt gaacctgcca
     6721 taactctcaa cctgctcagt ggtcaaaaaa tcaacgggca tatcggcctc cctgcctgac
     6781 ggctttttta acacaactgc aaccgttcga aatattataa attatcggac atcgtaaaac
     6841 tgttacatta atatgtctat taaatcgtaa atttgtaata atagacatga gttgtccgat
     6901 attcgattta aggtacattt ttatgcgact ttttggttac gctcgggtct caaccagtca
     6961 gcagtctctt gatcttcagg tcagagcact caaagacgca ggtgtgaaag caaaccgtat
     7021 atttaccgat aaggcatccg gcagttcaac agaccgggaa gggctggatt tgctgaggat
     7081 gaaggtggag gaaggtgatg tcattctggt taagaagctc gaccgtcttg gccgcgacac
     7141 tgccgatatg atccaactga taaaggaatt tgacgctcag ggcgtggcag tccggttcat
     7201 tgatgacggg atcagtaccg acggtgatat ggggcaaatg gtggtcacca tcctgtcggc
     7261 tgtggcacag gctgaacgcc ggaggatcct agaacgcacg aatgagggcc gacaggaagc
     7321 aaagctgaaa ggaatcaaat ttggccgcag gcgtaccgtg gacaggaacg tcgtgctgac
     7381 gcttcatcag aagggcactg gtgcaacgga aattgctcat cagctcagta ttgcccgctc
     7441 cacggtttat aaaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     7501 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     7561 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     7621 taaccctggt aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttt
     7681 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7741 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7801 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     7861 atgagcactt ttaaagttct gctatgtggt gcggtattat cccgtgttga cgccgggcaa
     7921 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     7981 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     8041 atgagtgata acactgctgc caacttactt ctgacaacga tcggaggacc gaaggagcta
     8101 accgcttttt tgggcactgt tgcaaatagt cggtggtgat aaacttatca tccccttttg
     8161 ctgatggagc tgcacatgaa cccattcaaa ggccggcatt ttcagcgtga catcattctg
     8221 tgggccgtac gctggtactg caaatacggc atcagttacc gtgagctgca ggagatgctg
     8281 gctgaacgcg gagtgaatgt cgatcactcc acgatttacc gctgggttca gcgttatgcg
     8341 cctgaaatgg aaaaacggct gcgctggtac tggcgtaacc cttccgatct ttgcccgtgg
     8401 cacatggatg aaacctacgt gaaggtcaat ggccgctggg cgtatctgta ccgggccgtc
     8461 gacagccggg gccgcactgt cgatttttat ctctcctccc gtcgtaacag caaagctgca
     8521 taccggtttc tgggtaaaat cctcaacaac gtgaagaagt ggcagatccc gcgattcatc
     8581 aacacggata aagcgcccgc ctatggtcgc gcgcttgctc tgctcaaacg cgaaggccgg
     8641 tgcccgtctg acgttgaaca ccgacagatt aagtaccgga acaacgtgat tgaatgcgat
     8701 catggcaaac tgaaacggat aatcggcgcc acgctgggat ttaaatccat gaagacggct
     8761 tacgccacca tcaaaggtat tgaggtgatg cgtgcactac gcaaaggcca ggcctcagca
     8821 ttttattatg gtgatcccct gggcgaaatg cgcctggtaa gcagagtttt tgaaatgtaa
     8881 ggcctttgaa taagacaaaa ggctgcctca tcgctaactt tgcaacagtg ccctgataaa
     8941 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     9001 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     9061 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     9121 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     9181 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     9241 gcgtcagacc ccgtatctca tataatgatt tcagtaaaac atactgaggc cggagaacgg
     9301 cgccgtttgt gctggttttt gttgcatctg gaggggattg cgcatggtgt gagtggctgt
     9361 tttttgatca ttttcacaaa aacgcaaatt tacagacttg acatgaaaag gggtctgacg
     9421 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9481 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9541 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9601 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9661 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9721 atttatcagg gcactgttgc aaagttagcg atgaggcagc cttttgtctt attcaaaggc
     9781 cttacatttc aaaaactctg cttaccaggc gcatttcgcc caggggatca ccataataaa
     9841 atgctgaggc ctggcctttg cgtagtgcac gcatcacctc aatacctttg atggtggcgt
     9901 aagccgtctt catggattta aatcccagcg tggcgccgat tatccgtttc agtttgccat
     9961 gatcgcattc aatcacgttg ttccggtact taatctgtcg gtgttcaacg tcagacgggc
    10021 accggccttc gcgtttgagc agagcaagcg cgcgaccata ggcgggcgct ttatccgtgt
    10081 tgatgaatcg cgggatctgc cacttcttca cgttgttgag gattttaccc agaaaccggt
    10141 atgcagcttt gctgttacga cgggaggaga gataaaaatc gacagtgcgg ccccggctgt
    10201 cgacggcccg gtacagatac gcccagcggc cattgacctt cacgtaggtt tcatccatgt
    10261 gccacgggca aagatcggaa gggttacgcc agtaccagcg cagccgtttt tccatttcag
    10321 gcgcataacg ctgaacccag cggtaaatcg tggagtgatc gacattcact ccgcgttcag
    10381 ccagcatctc ctgcagctca cggtaactga tgccgtattt gcagtaccag cgtacggccc
    10441 acagaatgat gtcacgctga aaatgccggc ctttgaatgg gttcatgtgc agctccatca
    10501 gcaaaagggg atgataagtt tatcaccacc gactatttgc aacagtgccc aaaaaagcgg
    10561 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggcagcagtg ttatcactca
    10621 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    10681 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    10741 cttgcccggc gtcaacacgg gataataccg caccacatag cagaacttta aaagtgctca
    10801 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    10861 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    10921 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10981 gaaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt taccagggtt
    11041 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    11101 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
    11161 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaattt tataaaccgt
    11221 ggagcgggca atactgagct gatgagcaat ttccgttgca ccagtgccct tctgatgaag
    11281 cgtcagcacg acgttcctgt ccacggtacg cctgcggcca aatttgattc ctttcagctt
    11341 tgcttcctgt cggccctcat tcgtgcgttc taggatcctc cggcgttcag cctgtgccac
    11401 agccgacagg atggtgacca ccatttgccc catatcaccg tcggtactga tcccgtcatc
    11461 aatgaaccgg actgccacgc cctgagcgtc aaattccttt atcagttgga tcatatcggc
    11521 agtgtcgcgg ccaagacggt cgagcttctt aaccagaatg acatcacctt cctccacctt
    11581 catcctcagc aaatccagcc cttcccggtc tgttgaactg ccggatgcct tatcggtaaa
    11641 tatacggttt gctttcacac ctgcgtcttt gagtgctctg acctgaagat caagagactg
    11701 ctgactggtt gagacccgag cgtaaccaaa aagtcgcata aaaatgtacc ttaaatcgaa
    11761 tatcggacaa ctcatgtcta ttattacaaa tttacgattt aatagacata ttaatgtaac
    11821 agttttacga tgtccgataa tttataatat ttcgaacggt tgcagttgtg ttaaaaaagc
    11881 cgtcaggcag ggaggccgat atgcccgttg attttttgac cactgagcag gttgagagtt
    11941 atggcaggtt cactggcgaa cccgatgaac ttcagctggc gcgttatttt catcttgatg
    12001 aagcggataa agaatttatc gggaaaagcc ggggtgatca caatcgactt ggtattgccc
    12061 tgcaaatcgg gtgtgtgcgt tttctgggca cttttcttac tgacatgaat catattcctt
    12121 ccggcgtccg gcattttacc gccagacagc tcgggattcg tgatatcacc gttcttgcag
    12181 aatacggtca gagggaaaat acccgccgtg agcatgcagc gctgatacgt cagcactatc
    12241 agtatcgtga atttgcctgg ccctggacat ttcgccttac ccgtctttta tatacccgga
    12301 gctggataag caacgaacgt cctggcctgc ttttcgacct ggcgacaggg tggcttatgc
    12361 aacatcgtat tattctcccc ggagccacca cgctgacccg gttgatttca gaggtaaggg
    12421 aaaaggcgac gttgcgcctg tggaacaaac tggcactgat accgtcagcc gaacagcgtt
    12481 cacagctgga gatgctgctg gggccaactg attgcagccg cctgtcttta ctggaatcac
    12541 tgaaaaaagg ccctgtgacc atcagtggtc cggcgtttaa tgaagcaatt gaacgctgga
    12601 aaactctgaa cgattttggc ctgcatgctg aaaacctgag tacactcccg gctgtgcgcc
    12661 tgaaaaatct cgcacgttat gctggtatga cttcggtgtt caatattgcc aggatgtcac
    12721 cgcagaaaag gatggcggtt ctggttgcct ttgtccttgc atgggaaacg ctggcgctgg
    12781 atgatgcact ggacgttctg gacgccatgc tggccgttat catccgtgac gccagaaaga
    12841 ttgggcagaa aaaacggctc cgctcgctga aggatctgga taaatctgca ttggcgctcg
    12901 ccagcgcatg ttcgtacttg ctgaaagaag aaacaccgga cgaatcgatt cgtgctgagg
    12961 tgttcagcta catccctagg caaaagctgg ctgaaatcat cacgcttgtc cgtgaaattg
    13021 cccggccctc agacgataat tttcatgacg aaatggtgga gcagtacggg cgcgttcgtc
    13081 gtttcctgcc ccatctgctg aataccgtta aattttcatc cgcacctgcc ggggttacca
    13141 ctctgaatgc ctgtgactac ctcagccggg agttcagctc acggcggcag ttttttgacg
    13201 acgcaccaac ggaaatcatc agtcagtcat ggaaacggct ggtgattaac aaggaaaaac
    13261 atatcacccg aaggggatac acgctctgct ttctcagtaa actgcaggat agtctgagac
    13321 ggagggatgt ctacgttacc ggcagtaacc ggtggggaga tcctcgtgca agattactac
    13381 agggtgctga ctggcaggca aaccggatta aggtttatcg ttctttgggg cacccgacag
    13441 acccgcagga agcaataaaa tctctgggcc atcagcttga tagtcgttac agacaggttg
    13501 ctgcacgtct tggcgaaaat gaggctgtcg aactcgatgt ttctggcccg aagccccggt
    13561 tgacaatttc tcccctcgcc agtcttgatg agccggacag tctgaaacga ctgagcaaaa
    13621 tgatcagtga tctgctccct ccggtggatt taacggagtt gctgctcgaa attaacgccc
    13681 ataccggatt tgctgatgag tttttccatg ccagtgaagc cagtgccaga gttgatgatc
    13741 tgcccgtcag catcagcgcc gtgctgatgg ctgaagcctg caatatcggt ctggaaccac
    13801 tgatcagatc aaatgttcct gcactgaccc gacaccggct gaactggaca aaagcgaact
    13861 atctgcgggc tgaaactatc accagcgcta atgccagact ggttgatttt caggcaacgc
    13921 tgccactggc acagatatgg ggtggaggag aagtggcatc tgcagatgga atgcgctttg
    13981 ttacgccagt cagaacaatc aatgccggac cgaaccgcaa atactttggt aataacagag
    14041 ggatcacctg gtacaacttt gtgtccgatc agtattccgg ctttcatggc atcgttatac
    14101 cggggacgct gagggactct atctttgtgc tggaaggcct tctggaacag gagaccgggc
    14161 tgaatccaac cgaaattatg accgatacgg caggtgccag cgatcttgtc tttggccttt
    14221 tctggctgct gggataccag ttttctccac gcctggctga tgccggtgct tcggttttct
    14281 ggcgaatgga ccatgatgcc gactatggcg tgctgaatga tattgccaga gggcaatcag
    14341 atccccgaaa aatagtcctt cagtgggacg aaatgatccg gaccgcaggc tccctgaagc
    14401 tgggcaaagt acaggcctca gtgctggtcc gttcattgct gaaaagtgaa cgtccctccg
    14461 gactgactca ggcaatcatt gaagtggggc gcatcaacaa aacgctgtat ctgcttaatt
    14521 atattgatga tgaagattac cgccggcgca ttctgaccca gcttaatcgg ggagaaagcc
    14581 gtcatgcagt tgccagagcc atctgtcacg gtcaaaaagg tgagataaga aaacgatata
    14641 ccgacggtca ggaagatcag ttgggagctc tggggctggt cactaacgcc gtcgtgttat
    14701 ggaacactat ttatatgcag gcagctctgg atcatctccg ggcgcagggt gaaacactga
    14761 atgatgaaga tatcgcacgc ctctccccgc tttgccacgg acatatcaat atgctcggcc
    14821 attattcctt cacgctggca gaactggtga ccaaaggtca tctgagacca ttaaaagagg
    14881 cgtcagaggc agaaaacgtt gcttaacgtg agttttcgtt ccactgagcg tcagacccct
    14941 ataactcaat gagaaaaaag aattaaaggt aaggagacgc tcatgagacg ttccggcagc
    15001 aaactgtgtc ttatcgccat gataacagcg acagcaactc tcactgcctg tgctccaaag
    15061 ggcagcgtgg aacagcatac ccggcattac gtatatgctt cagatgacgg ttttgatccc
    15121 aatttttcca cccagaaagc tgacacaaca cgaatgatgg tgcctttttt ccggcagttc
    15181 tgggatatgg gcgcaaagga taaagcgacc ggaaaatcac ggagtgatgt gcagcaacgc
    15241 attcagcagt ttcacagtca ggaattttta aactcactcc aaggcacaac acaatttgcg
    15301 ggtaccgatt accgcagcaa agacctcaca ccgaaaaaat caaggctact ggctgacacc
    15361 atttcagctg tttatctcga tggctatgaa ggcagacagt aagtggattt accataacgc
    15421 gtcattacgc gcaccgctca gacgggctca gggcgtttcc ggcggctacg ggataaaaca
    15481 ggcaacaacc cccccggaaa actgccggaa acgcgcccga taaattttat cctaatgcat
    15541 gcctatgcac gcaactgcat ggcatatgtt ttgacgtaaa atggatgccg taagcccttt
    15601 tttaccgact ttctgcttca tcagcctgac tgacacgacc atattctttc agaccgcccc
    15661 ctgctaaact tccttcatat tgccgcatta acccgggagg ctccgcaatg agcaacacta
    15721 cacactacga aaacgccaac tttcttcggg aactggctga aagcctgccc cgcatactgc
    15781 cggaaggtgg cccggacaaa gctgcgctgt tgcagcgact ggctaacgaa gaactggccc
    15841 aggcagagta tgaagatcag gttcgcgcca aagtgaccgc cgcccgtgct gacacgcgtc
    15901 cgggtatgac cacggaacaa ctccgccagc gcctgcatgg ccgttatcag gaactgcgcg
    15961 atgccgtatg acgtggaatg gaagcaggga gccatcgagg atgtaacgac gctgtttgac
    16021 tatatcgcgg aaaattcatc cctgtgggat gcgcagaacg ttaccgaccg ggttctcgct
    16081 gcggcggaca aactggccga cttcccgcga ctctatgaag tggactcacg ctatggcgag
    16141 gacgttcgcc ggatcagtct gatgggacag catgtcctct atgatgttga tgaccagacc
    16201 agaaaagtcc gggttctggc cgtggtcggc cagcgccaga atccccatat tgttctctaa
    16261 taaccggtga cggggacgca attcattgcg tggccgtcgc cggttggaca agcacagcgc
    16321 gtcagcgttt tccccacggc caccaccggc gcggtgttcc ggccggggtc tctgctgtct
    16381 ccggtttgtg ttccagcagt ctcatggact gcatttcctg ccgtagcccg gccacctcct
    16441 ccctgagtgc ttttatctcc gcgatcaacg cgtcggaggc cattttttca gtctcacccg
    16501 acagtctcaa ctcgtcccgc tcgtgagtct catcctgtct taactcaccg tacacccgga
    16561 taagctcgct ggtatccacc acccggccgc catcagcttc cgtacggtat gatacgcggc
    16621 ctttcgccat atcccgatac agggaacttc gggctttacc cgtcagttcc gtcgcctgtc
    16681 tcaccgtcac ccacgccatt gtctcacccc gctatttttg agacagtata cctgacaggc
    16741 gcacaccatg cccggaagga aagaatcatg acaaaacagg aaaaaacagc cctcaatatg
    16801 gctaaattta ttaaggagca gtcgctgcta ttgctcgata aactgaatga gctggatatg
    16861 gataccccgg ccgacatgtg cgagcggcta cacgaggatg cggaaacact ctggcaggtg
    16921 atgcgggata agctcgggga ggaataacag caccggcatg gccggtgctg gcatttattt
    16981 tttgccgaac ggccaccacc ggcgggcagg tttgtcctcc ggcagttcag gtaccggcac
    17041 gttaaaatag cggctgttat attcccgcat cagctccgct ttcagcttct cgttcaccgc
    17101 gctttccccg tgccgctcac tcaccacgtc aatcatcgcg ttaatctgct ccggtgagca
    17161 cttcccggca tcaaccacct gcacaccgtt ctcgatggcg aaccgctcca ccttgcgata
    17221 gaggtaatca cacagcttca ttcctgtggg tttgttccgt tcccattgcg gatttttacc
    17281 agtgaaaaag tacgtggctt tttcgtcgct gttgagccgc tcgtcataca tcccgatgga
    17341 tttcatcatc gccagatgct gggcccggtc gacaaaacag aaaacatacg ccccggtctg
    17401 ccaggcgagc accaccatat cttccagatg ctcgctggtc ggcatgatgc agcgctcgcc
    17461 gttccggtcc tccgcgtctt caataaatac catgtacagc gccataatcc ctcactccgt
    17521 tgtcataaat ttaaagcacg caaacaaccg ttcacgatcc agctctgccg ttttgcgggc
    17581 ctcgccggtc tgtgggttca cgatttcatc caccgacagg attgcccgct gcaccctgac
    17641 ctgcgtcgcc cggtccgggc agatgtacca gatttcatcg atgccgttag ctttgcggtt
    17701 gaacagatac ccggacacca cttcctgata ccggcgacgg ctcttgacgg tgcgctccac
    17761 ctcaaacgcc accgttttac cgtccagctc cgccagggcg tccggcactt tcatgccttt
    17821 aagactcaga cggtgcagaa tgcgccagcc ggtggcccct ctggcttcca tatcaaggcg
    17881 cagggattgc acggccagcc ggtgcggcag atgtgctgcc gacacccttg acggctgaaa
    17941 cggaataaca tccgccagcg gttcgtcctc gtcgaacgag aacgccacgc catgcggcgt
    18001 cagcccccac acgctgacct gacggccgtc caccggatat ttggtccgga ccagcagccc
    18061 tttcgccacc aggctgtcga ggaagcggta gaccggctgc accgccgcaa tccccagccg
    18121 ccgcgcaacc agctccgccg tggaacacag ctcttcccgc aaccagcgca gggtcagccg
    18181 ggttttctcc gcgttccgcg cctgtcgttt cgcgtggctg acgttgtaca gctccagcgc
    18241 catcagtaaa tccccgcaaa atcatcatcc gctttcggcc cgccgccgtc ggccgggggc
    18301 cggaactccg ccaggggatc ggttttcgcc agcaccggga aacgctgcaa cgggatttcc
    18361 cggcgctcaa cccgcagcgg actgctgaac gccatccggg ccacacccag cccggtcatg
    18421 accgcgaacc cgtccggcag gtgttgcagg gtgttcacgt caaacagcgg ccgcgcctcc
    18481 ttctggatga agctgtcccc gctgatatgc tccacgttcc ccgcctcccg gctggcacgg
    18541 cggcgctcca cgtccaccct gatttccccg gtctgccccg ccgcccactg cgcggactcc
    18601 aggctggccg cccggtaaaa ccagcggatc ggcgtgttat ccagtaccgt ggttttcaca
    18661 aattcagcgg gcagatcctg cccgcactgc ccgaaatcgc ccagcgactg gtgcgcgagc
    18721 aacagattgc agtcccggct gcgcagcgtc cccagtgcgt tcagtacata cttcgacagc
    18781 agatatttaa tttcatcgag catcacgctg acgtgtggcc actcccggaa ctcgtcccgc
    18841 gcaatgatga tttgcgcgca gcgggcaaac agcatttttt gcacacggat caccgcctcg
    18901 tcgtccatcg agccgataac gtacaggcag ccgccgccgt tgataatccc cgccacatcg
    18961 atgccactgt ccgtctgcag gacgctcagg tcagcgacgt tttccagcct agtgatcaac
    19021 cccttcacgt cctttttcag ggcctccggc agcgcgtacg ccgccgccag cacctgctga
    19081 atgtttgccc cctgcggaaa ctgctccgca atcagcttcg ccgctttctg ctcgctgatg
    19141 cggtagtggt ccgccacgtc gccggtctcg cccaggttaa gcccggcaat cagcaggttt
    19201 ttcagcgcgt actggtcgag atcgcggaac agattcagct gcgccggttt ccccggccgc
    19261 agatccagca gggtgaagtc cggacagtgc gcccggaaga cgtgcggcgc ccatgcgtcc
    19321 cctttggggt cgaagtacac caccgcgtcc ccgaaatcgc gtacgcagcg aatgagcgcg
    19381 ttggtggcca tcacgctctt gccgctcccg ggcaggccga ggatctgaat gttggtcttg
    19441 cgccaggtat cacgcggaac cagcaccggc ttgccctgct catccttacc cagaaacacc
    19501 gcctgctcct gccgggcctg ctccgaggaa aaatcggcca gcgcattaac cggtaccggc
    19561 tccccgaaat gcaggtggcg cacgtcggtc atctctcctg tccgctggct gctgcgccgg
    19621 ctctcctgat tcagtttcgc gttcaccgcc tcgtcagcag ccgcaaaatg accgtcaagc
    19681 agcaggaacg ccagcagccg cgccaacaca atcccgacag ccagcccgcc cgcgcaccag
    19741 gcaatcccga acaggttagc cggctgcagc aggtaataat ctgcgtttgc gtagaggtcc
    19801 ggacgcagga cggtttctcc ccagctctcg ataaacacgg cgacaaccag cccccccaga
    19861 agggccagac cggccgcgcc ggtcagccag tacgcgtagt tccagacatg tcgacgtgcc
    19921 ctgaccatcc gcccgggcga atccagcgag gccagcaccg aaaatggcgc tggccgttta
    19981 ttatcgcgct tatagcgatc ggcatactcc cgtcgctcct tatgactcag ccccacaaaa
    20041 cgcatccgtc cggcccaaag ttcagccaga ccgaaaaaca ggccgataac taggaaagcc
    20101 ccggccagac cgtaatactc ctggtacagc cagctgaccc acggataaat ctgccgggcc
    20161 agctccggaa tgtgccactt ccaggcaagc gaatccgtat taaacatgtt ctctctcctt
    20221 ca
//
