LOCUS       NZ_CP011865            13716 bp    DNA     circular CON 30-NOV-2023
DEFINITION  Enterobacter asburiae strain ATCC 35953 plasmid unnamed 2, complete
            sequence.
ACCESSION   NZ_CP011865
VERSION     NZ_CP011865.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03742638
            Assembly: GCF_001521715.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 13716)
  AUTHORS   Li,G., Hu,Z., Geng,X., Chen,L., Zhu,B. and Wu,L.
  TITLE     High quality genome sequence of Enterobacter asburiae type strain
            ATCC 35953, an antibiotic bacterium isolated from human
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13716)
  AUTHORS   Li,G., Hu,Z., Geng,X., Chen,L., Zhu,B. and Wu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2015) Center of Laboratory Medicine, Key
            Laboratory of High Humidity Medicine PLA, Chengdu Military General
            Hospital, Chengdu Military General Hospital, Chengdu, Sichuan
            610083, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: HGAP protocol, SMRT Analysis v. 2.3.0
            Genome Coverage                   :: 85.08
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/30/2023 15:27:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,769
            CDSs (total)                      :: 4,656
            Genes (coding)                    :: 4,488
            CDSs (with protein)               :: 4,488
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 100 of 168
            Pseudo Genes (incomplete)         :: 68 of 168
            Pseudo Genes (internal stop)      :: 19 of 168
            Pseudo Genes (multiple problems)  :: 18 of 168
            Pseudo Genes (short protein)      :: 1 of 168
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP011865.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13716
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="ATCC 35953"
                     /isolation_source="physical"
                     /host="Homo sapiens"
                     /culture_collection="ATCC:35953"
                     /type_material="type strain of Enterobacter asburiae"
                     /db_xref="taxon:61645"
                     /plasmid="unnamed 2"
                     /collection_date="2014-12-01"
                     /collected_by="Gengmi Li"
     gene            join(13387..13716,1..216)
                     /locus_tag="ACJ69_RS25830"
     CDS             join(13387..13716,1..216)
                     /locus_tag="ACJ69_RS25830"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_component="GO:0009295 - nucleoid [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="WP_233424652.1"
                     /translation="MSLEINQIALHQLIKRDEQTLEVVLRDSLLEPTATVVEMMAELHR
                     VYSAKNKAYGLFSEESELADSLRLQRQGEEDFLAFSRAPPGVCATSWRNIRSPTAGSCC
                     FCHYRYAVRSSCSFIPIRMWSGWCPQSWTGSSATGRVLSGGSCPGRTGIFVRSPRVKTG
                     IMPAAVGTGLRGLLVAYP"
                     /name="nucleoid-associated protein"
     gene            370..993
                     /locus_tag="ACJ69_RS23390"
                     /old_locus_tag="ACJ69_23370"
     CDS             370..993
                     /locus_tag="ACJ69_RS23390"
                     /old_locus_tag="ACJ69_23370"
                     /inference="COORDINATES: protein motif:HMM:NF018510.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_233424653.1"
                     /translation="MMSVQASVTNHAAVYVGDNIILHHLFGHLSSRTPYGKYYRDRTVG
                     WSGIRIECMVKTLILEGKMAKKFGKRIQFDVADLREMLRAMCSQVPGFKKYMSEAHMKG
                     IRFAFFNGDNNIGLEEFDMTRGGSVYRIVPVYEGAKSSGVLQIVVGAVALVAAFFTAGC
                     EYGSLGGGDECNSHQRHVNFDRVGVSMMLVCCPDAHAPAILRRG"
                     /name="hypothetical protein"
     gene            1186..4806
                     /locus_tag="ACJ69_RS23395"
                     /old_locus_tag="ACJ69_23375"
                     /pseudo=""
     CDS             1186..4806
                     /locus_tag="ACJ69_RS23395"
                     /old_locus_tag="ACJ69_23375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063066.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /name="DUF1983 domain-containing protein"
     gene            5057..5698
                     /locus_tag="ACJ69_RS23400"
                     /old_locus_tag="ACJ69_23380"
     CDS             5057..5698
                     /locus_tag="ACJ69_RS23400"
                     /old_locus_tag="ACJ69_23380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048965316.1"
                     /translation="MSGFEVYNSAGKLLVDSQNRSTLFYDQRALGAVTDKGYYRVDSPF
                     GNGSTLGITQPQFWNDGNLRWLQLDTNKYGLPGADLLEDNAGSMIRTSRNVGMQSGYLD
                     VFDSAGNLIWSAASASKMPRVVGFFDVPANYDLQNNTFAINLGFNPWILVNNCPGNLSD
                     DGTVVGYSGIVLKWTGSQLQGRYITKNQRNWSQTLQGRGLRIPIAQFVGI"
                     /name="hypothetical protein"
     gene            complement(5663..6025)
                     /locus_tag="ACJ69_RS25420"
                     /old_locus_tag="ACJ69_23385"
     CDS             complement(5663..6025)
                     /locus_tag="ACJ69_RS25420"
                     /old_locus_tag="ACJ69_23385"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_054830174.1"
                     /translation="MKRLFAMALLLAAGCADKDRDYAFKMDYPVDAARLSLGGDIHVNI
                     DCATREVNVISDSSNGIFSRHINKRLSNICYKKTDKLDVVYRFEPAKGVKQNMIATQYP
                     RVPPISNSDKLSDGNS"
                     /name="hypothetical protein"
     gene            6152..7440
                     /locus_tag="ACJ69_RS25835"
                     /pseudo=""
     CDS             6152..7440
                     /locus_tag="ACJ69_RS25835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063359.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /name="phage tail protein"
     gene            7497..8240
                     /locus_tag="ACJ69_RS23415"
                     /old_locus_tag="ACJ69_23400"
     CDS             7497..8240
                     /locus_tag="ACJ69_RS23415"
                     /old_locus_tag="ACJ69_23400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_059347888.1"
                     /translation="MQVGAFGLGNSSAQVITDVWDKSLGSRFIMMSPSTSGGPQYYSMG
                     IRISERSWGNGPNDVSQQSFSAFSMGGKRFTWMTMADGVNSGWLEVYHNGNTTKSSDGT
                     LKAASPVIKLFSDGRYLTNDESEGCTVTRLAKGEYLVEGCEGLNSDAAWGGIDGGFDIP
                     TDRNKQPLIWLDYDVNADGSVLVKTYHRTYPDAPTFARNELLGVDDGAPVDIPRDQFVS
                     IRVEMPADSIWNQRQKYTTRAPVKE"
                     /name="hypothetical protein"
     gene            complement(8302..9309)
                     /locus_tag="ACJ69_RS25085"
                     /old_locus_tag="ACJ69_23405"
     CDS             complement(8302..9309)
                     /locus_tag="ACJ69_RS25085"
                     /old_locus_tag="ACJ69_23405"
                     /inference="COORDINATES: protein motif:HMM:NF025253.3"
                     /GO_component="GO:0098024 - virus tail, fiber [Evidence
                     IEA]"
                     /GO_process="GO:0046718 - viral entry into host cell
                     [Evidence IEA]; GO:0019062 - virion attachment to host cell
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="WP_126296440.1"
                     /translation="MNTVDKKTGGNVSGSLMVTQGNSIGVSTQEGGDKAVKLYNITGDG
                     TVGSYVNAVGGAWYNGNWSLGGVRGSGTNLDRAQLNVNSGTGTAGSFLFYPDERFKSSS
                     CGADGAGYGGSWSDINTWQRNISFFRGNVSVNNDAGFIPFARWHSQCSGGYSSTVGLGS
                     IATGPSSWADVAITTLGDGGSAGQRIFQFTTANGDIYANAGGNLSGNYIFQKQPNCDIT
                     LKHDIKYDDGYQSYENIKKFLPATYVYNDDPRERVRRGVIAQDVMKIDSEYVKLVPAAP
                     KFDSEGSRVDADDTLALDTNVIMLDTVLALNYVIKKLEATQNELEELKLKIAAS"
                     /name="tail fiber domain-containing protein"
     gene            9460..10006
                     /locus_tag="ACJ69_RS23425"
                     /old_locus_tag="ACJ69_23410"
                     /pseudo=""
     CDS             9460..10006
                     /locus_tag="ACJ69_RS23425"
                     /old_locus_tag="ACJ69_23410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240123.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     gene            complement(<10137..10324)
                     /locus_tag="ACJ69_RS25840"
                     /old_locus_tag="ACJ69_23415"
                     /pseudo=""
     CDS             complement(<10137..10324)
                     /locus_tag="ACJ69_RS25840"
                     /old_locus_tag="ACJ69_23415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460030.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /name="DinI-like family protein"
     gene            10424..>10659
                     /locus_tag="ACJ69_RS25845"
                     /pseudo=""
     CDS             10424..>10659
                     /locus_tag="ACJ69_RS25845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240121.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            10663..10983
                     /locus_tag="ACJ69_RS23435"
                     /old_locus_tag="ACJ69_23420"
     CDS             10663..10983
                     /locus_tag="ACJ69_RS23435"
                     /old_locus_tag="ACJ69_23420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_054829880.1"
                     /translation="MGFPSPAMDYQEQRMTIDVICGVDNNCRVIETSCGWAVINVSLKP
                     EGGDTLLVSMDGRNQFVKLMGHALITQDGEAIEGEALNDVTVHGVLTHTLNQVKDDKSP
                     VI"
                     /name="hypothetical protein"
     gene            complement(11089..11164)
                     /locus_tag="ACJ69_RS23440"
                     /old_locus_tag="ACJ69_23425"
     tRNA            complement(11089..11164)
                     /locus_tag="ACJ69_RS23440"
                     /old_locus_tag="ACJ69_23425"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11128..11130),aa:Pro,seq:ggg)
                     /name="tRNA-Pro"
     gene            complement(11237..12076)
                     /locus_tag="ACJ69_RS25850"
     CDS             complement(11237..12076)
                     /locus_tag="ACJ69_RS25850"
                     /inference="COORDINATES: protein motif:HMM:NF013080.3"
                     /GO_function="GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="WP_233424650.1"
                     /translation="MALLWHFSRLHGRRTVYPHACGADHRLNQQGYQLGLFSSDGFNSP
                     LYRQALLSDFSLPTAQKQSDAQTASQWINWLQRYAQEDNRWFSWVAFNGTTLADSNKSD
                     FARRYGRAAGDVDTQIGRVLDALRESGKLDNTVVIVTAGHGIPLGDETKSMSWSRPNLQ
                     VPLVIHWPGTPAQRINMLTEHKDVMTTLMQRLLHVSTPANEYSQGQDLFSAARRHSWVT
                     AAGNNTLVVTTPKLTLVLNSNGNYQTYNLQGERLKDQKPQLSLLLQVLTDEKRFIAN"
                     /name="sulfatase-like hydrolase/transferase"
     gene            complement(12250..12423)
                     /locus_tag="ACJ69_RS25855"
     CDS             complement(12250..12423)
                     /locus_tag="ACJ69_RS25855"
                     /inference="COORDINATES: protein motif:HMM:NF023321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="WP_233424651.1"
                     /translation="MRTSYRPITMQRANLPLSYPMTARRFLEKHGLLDAQEYQRRLIEQ
                     RQPGSRQRSVVL"
                     /name="DUF3413 domain-containing protein"
     gene            complement(<12464..12550)
                     /locus_tag="ACJ69_RS25860"
                     /pseudo=""
     CDS             complement(<12464..12550)
                     /locus_tag="ACJ69_RS25860"
                     /inference="COORDINATES: protein motif:HMM:NF023321.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(13022..13252)
                     /locus_tag="ACJ69_RS23450"
                     /old_locus_tag="ACJ69_23435"
                     /pseudo=""
     CDS             complement(13022..13252)
                     /locus_tag="ACJ69_RS23450"
                     /old_locus_tag="ACJ69_23435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /name="YejL family protein"
ORIGIN
        1 gcggtgagat catcatgctc attcattccc atccggatgt ggtccggctg gtgccctcag
       61 agctggacag gatccagtgc gactggtcgg gtattgagtg ggggatcatg tcctggccgg
      121 acggggattt ttgtacgatc tccccgcgtg aagacaggga ttatgccggc ggcagtgggt
      181 actgggttac gcggactgct ggtcgcttat ccgtgaattt tatctgcgcg aatacggcat
      241 tggactcggg aactattcgg taccttacga atggtgggag agcggcaagg aacggctcta
      301 cgacgacaac tgggagcgtg agggatttgt tgagattgcc gccgggcaat gcagccaggg
      361 gacatcatca tgatgagcgt gcaggcatcg gtgactaatc acgccgcggt atatgtgggt
      421 gacaacatca ttctgcatca tctgttcggg cacctttctt cgcgaacgcc ttatggaaaa
      481 tattatcgcg acagaacggt cgggtggtca ggcataagga tcgaatgcat ggttaagacg
      541 cttattctcg aagggaaaat ggctaaaaaa ttcggtaaac gcattcagtt tgatgttgcc
      601 gacctgcgcg aaatgctcag ggccatgtgt tcacaggttc ccggattcaa aaaatatatg
      661 tcggaagctc atatgaaggg gatccgtttc gcctttttta acggtgacaa caatatcggg
      721 ctggaagagt ttgatatgac ccgcggtgga agcgtgtacc ggatcgtgcc cgtttatgag
      781 ggggccaaaa gttcgggcgt cctgcagata gttgtcggtg ccgttgcgct ggtcgctgca
      841 ttctttaccg cggggtgcga gtatggcagc ctggggggcg gcgatgagtg caacagccat
      901 cagcgccacg tcaattttga ccgggtcggg gtgtcaatga tgctggtgtg ttgtccagat
      961 gctcacgccc cagccatcct tcggcgcggg taaatcctcc agcacggaca acacgcctaa
     1021 ctacgccttc ggggcgcggt caatacggtc gctatggggc atcctgtccc cctggcctac
     1081 ggtctgatcg aggcaggggg agcgatagtc agcgccggta tgtactcgag cgatcagcag
     1141 taggccagcg gccactaact taaaggtgct tcggcatctt tttttatggg tgaaaaaatg
     1201 cagcttctta aacaagaaac catcctgcag ggtgccaaag ggggaggtgg cagttcacat
     1261 actccggttg agcagcctga cgatctgctg tcggtcgcaa aattaaaaat gctcattgcc
     1321 gtttctgagg gggaaataca gggcgacctg accgctcaga acatttttct caacgatacg
     1381 ccgctggcaa acgacagcgg ggaatacaac ttcagcggcg tgaaatggga gttccgcaag
     1441 ggcacacagg accagaccta tattgccggg atgccccagg tcgataacga gctggcggtg
     1501 ggtacaactg tcaccactac cgcgccctgg acacgccagt ttaccaatct ttccctggat
     1561 gccatccgca tcaagctcag ccttccggtc cagtatctct ataaagataa cggcgatatg
     1621 gtgggcacgg tcaccgaata tgcgatcgat ttatcaacgg acggcggtgc ctggaaaacg
     1681 gttgtaaacg gcaagtttga cggaaagacc acgacggaat atcagcgtga ccaccgtatc
     1741 gatctgccaa aatccacgtc cggctggtct gtcagggtca ggcgtattac ggctgatgcc
     1801 agcggatcaa attcgaaact ggttaacgcc ttcaaggtgt tttcgtatgc ggaagtcatc
     1861 gacagcaagc ttcgttatcc attaaccgcg ctcctgtatg tcgaagtgga cagcagccag
     1921 ttcaacggca gcgcaccgaa agtgacctgt aagataaagg gcaagctgat taaggttccg
     1981 gataattacg atccgacaac ccgaaaccta ttctggctca tggtccggcg ggttcaaaat
     2041 gcctggtccg aataaccctg cctggatatt ttacgatctg gttctggatg aaatttacgg
     2101 catgggcacg cgcgtggatg cgtccatggt ggataagtgg gcgctgtatt caatcgccca
     2161 gtactgtgac gaaatggttt cgacggggcc ggtggcaccg aacccgcgtt tcacctgcaa
     2221 cgttttcatt cagagccagg aggacgcctg gcaggtactt aacgatctcg ccgcggtatt
     2281 tcgtggaata accttctggg gcaacgatca gatttatgtc caggcagacg tcccgcagga
     2341 cgatgttgac tgggttctat aacgtctcaa acgttatcga tgggctgttt acttatgcgg
     2401 gcggctcata caagaatcgc tacagctcct gtctggtgtc ctggtccgat ccgcagaacc
     2461 attacagcga taccgttgag gggtttacga ttctgcgctt gtagaacgtt acgacgtccg
     2521 gcagacgtcc ctgaccgcaa tcggctgcac ctcgcaaagt gaagcgcacc gacgcggtcg
     2581 ctgggtattg ctctccaatg ccaaagacgg gactgtatcg tttggcgtgg gctggacggt
     2641 tatatccctt tgcccgctga aattatcggt gtcgccgatc ctttccgctc tggtaaggag
     2701 aacgggggcc gcataagcgc ggtcaatggc cgccagatta ccctcgatcg agaaatagac
     2761 tacgcggcga aagaccggct ggtggttaac cttccgacgg aaagcccaga cgcggacaat
     2821 cagcgcggtg agcgccgata aaaaaacggt gacggtggct acagcattca gtcaggttcc
     2881 tgtggcgggc gctgtctggg cgatagacag tgataacctc gcaatacagt atttcagggt
     2941 cacctcaatc gcggctaacg acgatagcac aggcggtttc actattacgg ccgttcagca
     3001 cgatccaaac aaatattcgt tacatcgatg acggcgttcg ggtcgagtcg cccccgatta
     3061 ccgtcacgcc gataagcgtc ctgtctgctc cgaagaatat cgtggtgact gagagcgatc
     3121 atgtgtctca ggggctgact gtagcaagcc tggacgtgtc atgggataag gtggagggcg
     3181 caatccgtta cgtcgcacag tggcgcaagg acaacggaga ctggataaac gttccggtta
     3241 ccagcgcgca gggtttctcg gttcagggca tttattcggg cggctatgac gtgcgcgtac
     3301 gggcgctgaa tgcgccagga tacgtcgtca ccatggggat acggtgaaac aacttatctc
     3361 tcaggtaaaa cgggaaaacc gggtactccg ctcaacttcc tggcgaccga agatgtggtc
     3421 tggcatatcg acctgacctg gaaatttccg gatggctcag gcgacacggc ctatacagag
     3481 attcagcgcg ccacaactgc cgactacgcc aatcctgaac tgctggtcct ggtgccgtac
     3541 ccggcttcag attatcagca tggtcccatg cctgccggcg ttcgccagtg gtaccgcgcg
     3601 cgcctgattg accgtatcgg taacgccggg gactggaccg actgggtcat gggcacgtcc
     3661 tcgatagatg tcagcgaaat aaccaatgac attctggagg atatgaaaga gtcggaaacc
     3721 ttcaaggacc tgatcgagaa cgcggtggac agcaatgaaa aaattgctgg catggctgac
     3781 gacatcaaac aggccaacga cgaactggag cagcaggcga aggatatcgc caaaaatgcc
     3841 caggacgtcg ggaaggttca gaccagcgtt aatgagcttt cgagcacggt cggtgaagtg
     3901 tcgtcttccc tctctgagct tgagcagacc gttgcgacgg ctgataccgc gctgggccag
     3961 cgaatcgaca gcatcagtgt gtctatggac ggcatgacgg gcggggtcaa gaactcagcg
     4021 attgccatta tccagaacgg gctggcgcag gtggctgcgc gtaaaaccct gtctgcatcg
     4081 gtcgccggca acagcgcgag tctggaccgt attgatgagg tgattgtcaa cgacagggag
     4141 gcaacagcgc gctctctgct gagcctgcag acggacgtca acggcaacaa ggcatccatc
     4201 aacagcctga atcagacgtt ctccgactat cagcaggcta cggccacgca gataaatggc
     4261 attacggcga ccgtgaacgg gcatacctcc gccatcacca ctaacgctca ggctatagcc
     4321 aacgttaatg gcgaactcag cgcgatgtac aacatcaagg ttggtgtctc cagcaacggg
     4381 cagtattacg ccgcggggat gggtatcggc gtggagaata cgccatccgg catgcagtcg
     4441 caggttatct tcctggctga ccgcttcgcc gtcactacgg cagcaggaaa cagcgtggcg
     4501 ttgccatttg tgatccagaa tgggcagacg ttcatcaggg ccagcttcat ccaggacggt
     4561 accattgaga acgccaaaat cggcaactat atccagtcga ataattatgt ggctggatct
     4621 gcaggctgga agcttgataa gggaggaacg ttcgaaatta acggagtggc cgggggcggg
     4681 aggatgctga tatccagcac tctcattcgc atctacgaca gcaacaacgt gctgcgtgtc
     4741 agaatgggtt atggtgatgc cacagggttt gcaatgctgg gacggtgcag gccgtattgc
     4801 cgttgattta agtgattatg ctatcccgat atattggaag cacgtctgta acatttgcag
     4861 cgggggaaac ggccaaggac gtttcctttt ccggggtaac gcaggacggg tcatttatat
     4921 caatcgtatc ggcgagttcg gcaggagtaa taaatgaata ttattgccgg gcttttaatg
     4981 gtggctttac cgttttttat ttaccgagcg gcggcagtat ttcgataact cttaatatgg
     5041 aggtttataa ctttcaatga gtggattcga agtttataac agcgctggaa aattgctggt
     5101 tgattcacaa aacaggtcga cactttttta tgatcagcgt gcgctcggcg cagtgactga
     5161 caaaggttat taccgcgtgg atagcccgtt cggaaacgga agcacgctgg gtattaccca
     5221 gccgcaattc tggaatgatg ggaatttgcg ctggctgcag ctggatacta acaagtatgg
     5281 cttaccaggc gctgaccttc tggaagacaa tgcgggcagt atgatacgca cgtccagaaa
     5341 cgttggaatg cagagcggat atttggatgt tttcgacagc gcggggaatc ttatctggag
     5401 cgccgcctcg gcatcgaaaa tgccgcgcgt ggtggggttt ttcgacgttc ctgccaatta
     5461 cgatctgcag aacaatacct ttgcgataaa cctgggtttt aatccgtgga ttctggttaa
     5521 caactgccca ggcaacctca gtgacgacgg aactgtcgtt ggttattcag gaatcgtact
     5581 caaatggaca ggctcacagc tgcaggggag gtacatcaca aaaaatcagc gcaactggag
     5641 ccagacactt caggggcgag ggctacgaat tcccatcgct cagtttgtcg gaatttgata
     5701 tgggtgggac gcgcgggtat tgagtagcga tcatgttttg cttcacacct tttgcaggtt
     5761 cgaagcggta taccacatca agcttatccg ttttcttata acagatattg cttagccgct
     5821 tatttatatg tcggctgaaa atgccattgc tgctgtctga aattacgtta acttccctcg
     5881 tggcacagtc gatatttacg tgaatatctc cccccagaga taagcgcgcc gcatccaccg
     5941 ggtaatccat tttaaaggca tagtctctgt ctttatcggc acagccagcc gccagcaaaa
     6001 gtgccatggc gaataatcgt ttcatttcta cattcctgta tctgcgggaa tatccatttt
     6061 attcgagttt aaaaaatagt cagattgata agagcgatca attttacatt attgatcgct
     6121 ttaaacgatc gttattatcg tgaggtagtt catgctttat aacactggca ctattgctat
     6181 caacggaaat accgccaccg gaacgggtac gaactggact gcgccagcca gccaggtccg
     6241 cgctggccag acgatcatcg tcatgtctaa cccggtgcag atgttccaga tttcatctgt
     6301 gaacagcgcc acgtcaatga cggttacgcc tgctgcttcc ccggcgctga gcggccagaa
     6361 gtatggaata ctggtgtccg acaacatctc tgtcgacgga ctggcacagg cgatgtcgca
     6421 gcttatcaaa gagtatgacg agaacattgg cgcgtgggag acgttcgcca ctacctcagc
     6481 aaaccagacc atcaccgtta ccatcaacgg aacctccgtt aatattccaa gtattggcgc
     6541 gcttgccaga aaggggtcca acagcgatat caccgagctg aaagggctca ctactgcgct
     6601 ttctattgca cagggcggga ctggggcaaa gaccgaagct gacgctcgcg caaacctcgg
     6661 tttgggaagt agcgccacga gaaacgccta cagcacctcc ggggatatgc tttccgttgg
     6721 tgattttgga gtggggactg tattaccaaa tgggcaagta ttcgcacaca gaatgacgct
     6781 accccatctg gtttttatgg ttctacgcag gatggatatt cagaaattgg tgtttctact
     6841 gacctgacta ctttgatcat caaccgaggt tccaggccaa ctcgtattca tcagtcttat
     6901 aacctcaggc gaacatggtt ctcgtattac agtggctcgt cctggtcata tcatgaggct
     6961 tacaccacgg gcaataccac cagagccagc gatggcacgc tcaaagcagc atcgcctgtg
     7021 gttaaagtgt tttcggatgg atcatgccag actaacgatg aatctgatgg ctgctctgtc
     7081 actcgtctgg gcatcggtga atacctggtt gaagggtgtg aggggcttaa ctctgatgcg
     7141 gcctggggtg gcatcgatgg cggttttgat atcccaaccg acagaaacaa acagcctctt
     7201 atctggctgg attatgaggt taacgcggat ggttctgtgc tggtaaaaac ctatcatcga
     7261 acacatcctg gcgcgcctgc attcgccagg aacgagttgc agggtatcag cgatggcgat
     7321 ccggtggata ttccacgcga ccagtttgtg tccgtccgtg tcgaaatgcc tgccgattct
     7381 ttatacaacc aaaaactcag ggacgacgaa ctggcaatga ctacggatga aggtgaataa
     7441 aggtcggttt gggaagtagc gccacgaagg acgtgggaac agctgcagga aacgtaatgc
     7501 aggtgggagc gtttggcctg gggaatagtt cagcgcaggt aattactgac gtgtgggata
     7561 aatcattagg cagcaggttt attatgatgt ctccatcaac ttcaggcggg ccacaatatt
     7621 actctatggg tattagaata tctgagcgca gttggggaaa tggcccaaat gatgtttccc
     7681 agcaatcatt ttctgcattc tcgatgggag gaaaaagatt cacatggatg acaatggctg
     7741 atggggttaa ttctgggtgg ctagaggttt atcataacgg caataccaca aagtccagtg
     7801 atgggacact gaaagctgcc tcgcctgtga ttaaactttt ttccgatggc agatacctga
     7861 ccaacgatga atcagagggc tgtactgtaa cccgtctagc caaaggtgaa tatctggttg
     7921 aaggttgtga agggctaaac tcggacgccg catggggcgg cattgatggt ggttttgata
     7981 tccccacaga ccgcaacaaa cagcccctga tctggctgga ttatgacgtt aatgctgacg
     8041 gttcagtgtt agtgaaaacc tatcaccgca cgtatccaga cgcgccaaca ttcgccagaa
     8101 atgaattact gggtgttgat gatggcgctc cggtcgatat tccgcgtgac cagtttgtca
     8161 gtattcgagt ggaaatgccc gcagacagca tctggaatca acgacagaaa tatacaacga
     8221 gagcccctgt aaaagagtaa ggccactgaa aaaatgcagc ccgtcattta ccggaacggg
     8281 ctgcagctga atgctcaggc ttcatgatgc cgctattttt agcttaagct cctccagctc
     8341 gttctgtgta gcctccagct tcttgatgac gtagttcagg gccagcacgg tatcaagcat
     8401 aatgacgttc gtatcaagcg ccaacgtatc atcagcatca accctgcttc cttcgctatc
     8461 aaactttggc gcagcaggga ccagcttgac gtactcacta tcaatcttca tgacgtcctg
     8521 agcgattaca cctcggcgaa ctctctcacg agggtcatcg ttgtagacgt aagttgctgg
     8581 caggaatttc ttgatattct cgtatgactg ataaccatca tcatatttaa tatcgtgctt
     8641 cagcgtaatg tcacagttag gctgcttctg gaaaatatag ttaccggaaa gatttccacc
     8701 agcgttggcg tatatatcgc cgtttgctgt cgtgaattga aatatacgct gtccggcaga
     8761 accaccatcc ccgagtgttg ttattgctac atccgcccac gaactaggcc cggtagctat
     8821 agaaccaagc cctactgttg aagaatatcc accactgcat tgactatgcc aacgggcaaa
     8881 aggaataaac ccagcatcat tgttaacgga tacgttaccc ctaaaaaaag agatatttct
     8941 ttgccatgtg ttaatgtctg accatgagcc accataacca gctccatcag ctccacacga
     9001 agatgatttg aatctctcat cggggtaaaa taaaaatgaa cccgccgtac cagttccgct
     9061 attaacattt aactgcgctc tgtctaaatt agtcccgctt ccccgaacgc caccaagaga
     9121 ccagtttccg ttataccagg cccctcccac agcgtttaca taacttccga cagtcccatc
     9181 ccctgtaatg ttgtatagct taacagcctt atcaccacct tcctgcgtgg ataccccaat
     9241 actattgccc tgagttacca tcaaactgcc ggaaacattt ccccctgttt ttttatcgac
     9301 agtattcaac cgcgaatcat ccccggccgc cacggttccg gcagccgttc caacgttcct
     9361 tgtggcgctg tttcccaaac cgacgtttta tagattgctc tcaagcggcc tggcgataac
     9421 ttcacctgat ttttttgctg aaataactgg gtgaaaaata tgcaaattgg ctatgtaagg
     9481 gtgtcaacaa atgaccaaaa tacggatctt cagcggcaag cgctcgaacg cgcaggatgt
     9541 gaacaatttt cgaagaaaaa atgagcggaa cggtagcaaa ccggccagca ctaaaaaagc
     9601 ttctcaaggc attaaatgag ggggatacgc tggtggtctg gaagctggat cggcttgggc
     9661 gcagcatgcg taatctggtg ctgctggtgg acgaacttcg gcagcgcggt atccacttta
     9721 agagcctcac cgacagtatc gatacatcaa gcccgatggg cgcttcatct tccatatcat
     9781 gtcagccctg gcggaaatgg agagggagtt gattgtggaa cgcacccggg caggtctggc
     9841 tgcagctcgt gagaaaggga gaatcggcgg caggcgtccg aagctcaccc aagagcaatg
     9901 ggctcaggca gggcggttga tagcgaacgg cgtagacagg aagcaggttg caataattta
     9961 tgacgttgca gtatgcacgc tatataaaaa atttcctgtg ggataaatca gccaagccat
    10021 ggtcgggcag tcagctaata ctgtcgctgc ccggccatat tagagcccag cctggcgaac
    10081 tgtcggaaat tcagaaccag ccacatatcg gcctcttcaa acatctcctc cagcatgcga
    10141 ttcaactttt cccgatcgct tttgctggcg tcactattca agccgttcgc ctgcatcggc
    10201 tttaccttca cttctgcatc aggaaaaatc tggtgcactc gcttgttagc tcagccagga
    10261 tgatctctct ggctccttgg agcccttcaa catttcgctt gtcataaacc agctcaacaa
    10321 acataccgat cctcttataa gtgaaaaatt gcctgtgctt gatctgtttt tataaaatac
    10381 tactgtatat gcatacagtc aataagtgag tgagggtgtg ttcatgcctc gtcaaccgga
    10441 tattcgtgct gcttttattg cggccataca gcaaacccga agggctatct ctgcctgcat
    10501 acagacaaat tcatcgctga actgcaggag aggcactggc atttcagcca agcggatgca
    10561 aattcatgga tcgagcgata ccagccggac ttcgccgata agacgacaaa cggaagtgag
    10621 aaccgatact ggatcctgcg taacgatggg gagggttttc taatgggatt tccatcgcca
    10681 gccatggatt accaggagca gcgcatgacg atagatgtta tctgcggtgt agataacaac
    10741 tgccgggtta ttgaaacttc atgcggctgg gccgttatta acgtcagtct gaagccagaa
    10801 ggaggggata cgttgctggt tagcatggac gggagaaacc agttcgtgaa gctaatgggc
    10861 cacgcgctga tcactcaaga tggtgaagcg atagaagggg aagctctgaa tgacgtaacg
    10921 gtacacggcg ttcttacaca tacacttaac caagttaaag acgataaatc gcctgtaata
    10981 tagcgtgtaa atgtcggggg ttattccccc atttttcccc aatgcttccc cgtacaaatt
    11041 ttaaacatga aaaaaccagc cgtaagaggc tggttttcaa tgtgtttttg gtcggcacga
    11101 gaggatttga acctccgacc ccgacacccc atgacggtgc gctaccaggc tgcgctacgt
    11161 gccgacgcat aaaaagaata ctactcgatt ccgttttgaa tgcaagggaa tctgtcgcta
    11221 actggtttat tattaatcag ttagcgatga agcgcttctc atccgtcagg acctgaagca
    11281 gcaggctcag ctgcggtttc tgatctttca accgttcacc ctgcagatta tacgtctgat
    11341 aattcccgtt gctgttcaga accagcgtca gcttcggcgt ggtgactacc agcgtattat
    11401 tccccgccgc cgtcacccag ctgtggcggc gcgcggcgct gaagagatcc tgcccctgcg
    11461 aatattcgtt tgctggcgtg ctgacgtgca gcaggcgttg catcagggta gtcatcacat
    11521 ctttatgctc ggtgagcata ttgatgcgct gcgccggggt gccaggccag tggatcacca
    11581 gcggaacctg cagattcggg cgagaccagc tcatactctt cgtctcgtca cctagcggga
    11641 tgccatggcc cgcggtcacg atcaccacgg tgttgtccag cttgcccgac tcgcgaagcg
    11701 cgtcaagcac gcggcctatc tgcgtatcaa cgtcgccagc cgcgcgaccg tagcggcgcg
    11761 caaagtcact cttattgctg tcagcaagcg ttgtgccgtt aaaggcaacc catgagaacc
    11821 agcggttatc ttcctgggcg tagcgctgca gccagtttat ccattggctg gcggtctgcg
    11881 catcggactg tttttgcgcc gtcggcagtg agaaatcgga cagcagcgcc tggcggtaga
    11941 gcgggctgtt aaagccatcg gaggagaaca accctaactg atagccttgc tgattcagcc
    12001 ggtgatcagc gccgcaggcg tgcgggtaga cagtacgccg tccatgtagc cggctgaaat
    12061 gccatagaag agccaaagat gcccgcatcg gcggtgttac cggagctcat atgctgagta
    12121 aacgagacgt tttgactcgc gaagcggcca gcgccggcat ctgcttctcg aaacgggaat
    12181 agttcaggcc atcgacggtg atcagcagga cgttttgccc gcgcccatat cgcggtagtt
    12241 cagtgtcgct cagaggacta ccgaacgctg acggcttccg ggttgcctct gctcgataag
    12301 acggcgctgg tactcctgcg catcaagcag gccgtgtttc tcgaggaagc gacgggccgt
    12361 catcgggtac gagagcggca ggttcgcgcg ctgcatggta atagggcgat aagaagttcg
    12421 catccgccca gatatacatg atgtgcgaac tgatagaaag agacgaaaaa gagcgcggcg
    12481 acgggcttcg cataatggcg tcgccggtca ggctgcggag cttttgccag ctccaggtag
    12541 cgaacagcat ctcaatcagc aggatcacca gggacgctga taaacatcag ctgccagtca
    12601 cgggcagttt cgttctggtc gggggttaat caccagttcc cagacgatgg ggttgaggtg
    12661 caggtgaagc gggtgaacac ttcactgtcg ataagccagc agcgtcatcc ccacccgtcg
    12721 caagaatggc ggacaagaac cgcatcagac gcctgcgaca tgacgataaa cgtcaagggg
    12781 aacagagatc agcagatagg tggcgaaaac caaaaagcta aagtgaccca gcaacgctca
    12841 tccaggagta gatacgcccg gttagcgttg tggccagtct gcgacaacag ataacgacag
    12901 ccaatgaccg tcgccagcag aatgttgaac aggagcaaac cagtgccccc agctgaccat
    12961 ctgggagact ttttcacggt agcgctgacg attcgtcacc ataaactgtt gttcgtatcc
    13021 cttaatgggc cgggtcgttg ctgacggacg actgtaaagc ttgagcgaac gattttgcga
    13081 tcgcctgacg ctgagccgga gcaacgctgg tgttaatcag gttggtgacc atatttccca
    13141 aaaccatcag ggagagatcg cgtcggcgtt ttattttttc cagtacgcgt tgaccagctc
    13201 actgagcagt tgttcaacgt ggttcatcac tgtagcggga atgttgtggc ataaatcaaa
    13261 atcagtttgt tgatgaaagg gcaacatatt acacgtagca acagcttttt tccccttttt
    13321 tatctgttgt tgcacacatg tcctggtggt ggttgaatac cgcccggtct aaaaggagag
    13381 tttatcatga gtctggaaat caaccagatt gccttgcacc agcttatcaa gcgtgatgag
    13441 caaacccttg aagtggtgct gcgcgattcg ttactggaac ctacggcgac cgtggtggag
    13501 atgatggcag agctgcatcg cgtctacagc gccaaaaata aagcctatgg cctgttcagc
    13561 gaagagagtg aactggctga tagcctgcgc ctgcagcgtc agggcgaaga ggatttcctg
    13621 gcgtttagcc gcgcgccacc gggcgtctgc gcgacgagct ggcgaaatat ccgttcgccg
    13681 acggcgggat cgtgctgttt ctgccactat cgctac
//
