LOCUS       NZ_CP011658             3223 bp    DNA     circular CON 08-FEB-2024
DEFINITION  Enterobacter hormaechei strain CAV1176 plasmid pCAV1176-3223,
            complete sequence.
ACCESSION   NZ_CP011658
VERSION     NZ_CP011658.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03733727
            Assembly: GCF_001874505.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Sheppard,A.E., Stoesser,N., Wilson,D., Sebra,R., Kasarskis,A.,
            Anson,L., Giess,A., Pankhurst,L., Vaughan,A., Grim,C.J., Cox,H.,
            Yeh,A., Sifri,C.D., Walker,S., Peto,T.E., Crook,D.W. and
            Mathers,A.J.
  TITLE     Rapid spread of a carbapenem resistance gene driven by multiple
            levels of genetic mobility
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Sheppard,A.E., Stoesser,N., Wilson,D., Sebra,R., Kasarskis,A.,
            Anson,L., Giess,A., Pankhurst,L., Vaughan,A., Grim,C.J., Cox,H.,
            Yeh,A., Sifri,C.D., Walker,S., Peto,T.E., Crook,D.W. and
            Mathers,A.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2015) University of Oxford, John Radcliffe
            Hospital, Oxford OX39DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP3 v. 2.2.0
            Genome Coverage                   :: 81X
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/08/2024 01:46:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,901
            CDSs (total)                      :: 4,784
            Genes (coding)                    :: 4,681
            CDSs (with protein)               :: 4,681
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 39 of 103
            Pseudo Genes (incomplete)         :: 61 of 103
            Pseudo Genes (internal stop)      :: 12 of 103
            Pseudo Genes (multiple problems)  :: 8 of 103
            Pseudo Genes (short protein)      :: 1 of 103
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP011658.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="CAV1176"
                     /isolation_source="Perirectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pCAV1176-3223"
                     /geo_loc_name="USA:Virginia"
                     /lat_lon="38.14 N 78.45 W"
                     /collection_date="2010-05"
                     /collected_by="Clinical Microbiology University of Virginia
                     Health System"
     gene            complement(join(2803..3223,1..65))
                     /locus_tag="AB284_RS24965"
     CDS             complement(join(2803..3223,1..65))
                     /locus_tag="AB284_RS24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(743..1066)
                     /locus_tag="AB284_RS00010"
                     /old_locus_tag="AB284_00010"
     CDS             complement(743..1066)
                     /locus_tag="AB284_RS00010"
                     /old_locus_tag="AB284_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1705..1875
                     /locus_tag="AB284_RS00020"
                     /old_locus_tag="AB284_00015"
     CDS             1705..1875
                     /locus_tag="AB284_RS00020"
                     /old_locus_tag="AB284_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004193911.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNWVHGAEIV
                     ENAYRVLEVKA"
                     /name="hypothetical protein"
     gene            complement(2084..2416)
                     /locus_tag="AB284_RS00030"
     CDS             complement(2084..2416)
                     /locus_tag="AB284_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(2584..2799)
                     /locus_tag="AB284_RS24975"
     CDS             complement(2584..2799)
                     /locus_tag="AB284_RS24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     ARG             complement(2085..2416)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          complement(1163..1354)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.821"
                     /coverage="89.0"
                     /evalue="7.28e-73"
     Typing          1334..1817
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.756"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          complement(2..753)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.016"
                     /coverage="100.0"
                     /evalue="9.32e-164"
     Typing          complement(2487..3223)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.903"
                     /coverage="100.0"
                     /evalue="3.98e-134"
ORIGIN
        1 gtagtctgct gctgcgcttt cgatttctgc tctaagtcct tcgccagcgt taaaagactg
       61 ttcatagatg gctcccttca gtcggatgtt tcgccccccg tccgggtcgg caatgctgat
      121 gctgcttttt gtggtgcgca ccacctcaaa acctgcgctt tccagcgcct cagtgacgtc
      181 ctgacgcgtt ttcagctccc cggatgaggc aagggcaagc aggccgctcg taatcgcctg
      241 ggcggcttcc tgcttcgctt ccggcagcgc ggaaggcgag accagcaccc gccggttctc
      301 cggcgcgttc gggtcatgca gccccagcct gccgttcact atggtctgcc aggcatcgat
      361 gcgcggacgg tcggcgcggt cgtaatacgg ctgtagccgc ttgccggtca gaagctccgt
      421 gttcgggatc aggaagttca gctccagccg ccccttgtcc cggtgctcaa cccacagcac
      481 gctgtactgg tctttatcga gtccgggcat cagaacccgc tcgaaactcg ccatcagctt
      541 ttcgcgctgg ccgggcggta aatcctgttc ggcaaaagac agaacgccgg aagtgtactt
      601 tttggcgtag ggcgaggcgt cgataagctc ccggacttca tccggcttcc cctgcagaac
      661 gctggcgccg tcgcgctggc gatctttacc cagcagataa tcgaccggcc cgccgccgcc
      721 gccgcgcccc cgaggatgaa acttaacgat catcatcagc acccttttcc agtacggcat
      781 gccgcaaccg ctcgagtccg gcatcgatgg ccatcagcgc ggcgacaatc tgcacgcggt
      841 cgtgtccgct tccaccaccg gcgttaacct ggcgggcaat ctggttaagg ttgttcccca
      901 tgccggcaag ctgacgaagc agcgccggcg agatcgacgg aagtttgccg gcgcgtgcgg
      961 gcttctcgtc caggcacgtc tgccgcatcc aggccgcgag ttgcttaccc tcgcagcgct
     1021 ccagcagacg ccggtgctcg tcctcagtca cccacatggt gagcattttg ctgcgtttgt
     1081 ctgccaatcc ggcctcctga taacgccggg acggacggga aatcttttcc cggtcggcac
     1141 ggtgttggac aagccgcaga cgcggcagtg gttttagcgg gtttcggggc gcagccctga
     1201 accagtcacg tagcgctagc ggagtgtata ctggctaaac atgttatgac ggagtggtaa
     1261 tttatgaaag tgcttcatgt gacaggggaa aaatgcggct gccgggcgta agtgcaaaat
     1321 gcaggtggtg atgaaatgac gcctcctcgc tcactcggtc gctacgctcc tgccgtgaga
     1381 ctgcggcggg cgttaccagc tcacagatta cgggataagc aaatgccgct cgaagcagga
     1441 aaggccaaga aaaggatgcg gagttgccgt ttttccatag gctccgcccc cctgacgagt
     1501 atcacgaaaa tgacgctcaa atcagcggtg gcgaaacccg atgcggctta aagataccag
     1561 gcgtttcccc ctggtggctc cctcgtgctc ccccgcattc ctgcctttcg gtttaccggt
     1621 gtcattccgc tgttatggcc gcggtttctc attccacgcc tgaaactcgg ttccgggtag
     1681 gcagttcgct ccaggatgga ctgtatgcac gaaccccccg ttcagtccga ctaccacgcc
     1741 cgttccggta actatcaact tgagtccaac ccggaaagac acgacaaatc gccagtggcg
     1801 gtagccattg gtaactgggt tcacggagca gagattgtag agaatgccta cagagttctt
     1861 gaagtgaaag cctgagtgcg gctacactga aaggacagtt taggtatctg cgctccgtga
     1921 aagccagtta ccatcggaac aaagagttgg tagctctgaa ttccggcaaa cgaaccaccg
     1981 ttggtagcgg tggttttttt gtttacaggg aaaaagatta cgcgcagaaa gaaaggatct
     2041 caaggaaatc cttttgcttt ggccaatatg cttcggtaaa aagttaatgc gcagaagatt
     2101 ttgaaaaaat attaataacc aaaactccaa cgcaaataag gaacattcct gcaattgcgg
     2161 gccaatcaag tttttgaccg ctaaccaacc aaccagctag gcttattagt acaattcccg
     2221 caccagacca aattgcgtaa gcaataccgg taggtatatt ttccaatgtt tgggaaagca
     2281 gataaaaggc agcgcaataa cttattgcag ttcctacaga cggccacagg cgtgtaaatc
     2341 cttctgaata tttcataaag gtggtgccta tgacttcagc cacaatagcc cctcccagga
     2401 aaatatatgt attcataaaa atacctcatg ttgaaaaatt taaatatctt tacataacaa
     2461 ctaattaata gaacccggct tatcatagcg aaggaccatg atgctgccac tgcgatcgtt
     2521 ccagctccag cgctttctgg tgctgcaccg cccgctcctg ctgccggtga gtttctatcg
     2581 cgttcagcct ttcgacaacc ggcgcagctg ctcgctcaaa ctctgcacct gcctgctcaa
     2641 gaccgtgacg cgctcgttca gcgccgcgtt ctcccgttga ataagaccag acatcgtccg
     2701 ccattccgcg aaggcgttct cccactcgtc cagccttttc gagtagtcct gctgtagctg
     2761 ctctaatgcg ctcagcaact gtttttccag ctccgtcatg tgctatttcc ccgccagtat
     2821 catccagctc gagtcctctc cgaactgtcc cgcttccggg ttcaccctca cgcagcggcg
     2881 tctctgctcg ccgcaggtcg agagctgtac gccgctgttc ctctctgaca gcatggcctg
     2941 cgtgctcttc tgctcccgga tggtcgtata gttctcgagt atctgctgcc cctgccacca
     3001 cagaatgccc gcgctcgacg ctatcagcag cgcggagacc agaacgatgg tcagccacgt
     3061 ctggctgacc atgcgcagca tccctttcgt gcgctggctc atggctgagg acagcttccg
     3121 gtcgtggtcg aggatggcgg cgctgattct cttctcgctt tcgctcagtt ccgctctgac
     3181 agacttctcg tgctcgctga atgcggcttt cagcatctcg ccg
//
