LOCUS       NZ_CP011651             1916 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Citrobacter freundii strain CAV1741 plasmid pCAV1741-1916, complete
            sequence.
ACCESSION   NZ_CP011651
VERSION     NZ_CP011651.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03733754
            Assembly: GCF_001022275.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Sheppard,A.E., Stoesser,N., Wilson,D., Sebra,R., Kasarskis,A.,
            Anson,L., Giess,A., Pankhurst,L., Vaughan,A., Grim,C.J., Cox,H.,
            Yeh,A., Sifri,C.D., Walker,S., Peto,T.E., Crook,D.W. and
            Mathers,A.J.
  TITLE     Rapid spread of a carbapenem resistance gene driven by multiple
            levels of genetic mobility
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Sheppard,A.E., Stoesser,N., Wilson,D., Sebra,R., Kasarskis,A.,
            Anson,L., Giess,A., Pankhurst,L., Vaughan,A., Grim,C.J., Cox,H.,
            Yeh,A., Sifri,C.D., Walker,S., Peto,T.E., Crook,D.W. and
            Mathers,A.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2015) University of Oxford, John Radcliffe
            Hospital, Oxford OX39DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP3 v. 2.2.0
            Genome Coverage                   :: 47X
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001022275.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 01:56:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,287
            CDSs (total)                      :: 5,166
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 33 of 122
            Pseudo Genes (incomplete)         :: 81 of 122
            Pseudo Genes (internal stop)      :: 22 of 122
            Pseudo Genes (multiple problems)  :: 14 of 122
            Pseudo Genes (short protein)      :: 1 of 122
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP011651.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="CAV1741"
                     /isolation_source="Respiratory"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="pCAV1741-1916"
                     /geo_loc_name="USA:Virginia"
                     /lat_lon="38.14 N 78.45 W"
                     /collection_date="2012-10"
                     /collected_by="Clinical Microbiology University of Virginia
                     Health System"
     gene            complement(2..115)
                     /locus_tag="AB183_RS26040"
     CDS             complement(2..115)
                     /locus_tag="AB183_RS26040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            816..1052
                     /locus_tag="AB183_RS00005"
                     /old_locus_tag="AB183_00005"
     CDS             816..1052
                     /locus_tag="AB183_RS00005"
                     /old_locus_tag="AB183_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031942344.1"
                     /translation="MTRLTNLTPAEKQFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRHADRQRALREEERQQAEFTWSRPRSPRR"
                     /name="hypothetical protein"
     gene            complement(1308..1610)
                     /locus_tag="AB183_RS00010"
                     /old_locus_tag="AB183_00010"
     CDS             complement(1308..1610)
                     /locus_tag="AB183_RS00010"
                     /old_locus_tag="AB183_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="WP_031942343.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIAAALLA
                     FFLGGFGAHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     Typing          477..650
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(7..491)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.648"
                     /coverage="80.0"
                     /evalue="0.0"
ORIGIN
        1 atcagttacc agtggctgct gccagtggcg cttttgcatg tctttccggg ttggactcaa
       61 gacgatagtt accggataag gcgcagcagt cggactgaac ggggggttcg tgcatacagt
      121 ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agaaaccgcg
      181 gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg
      241 agctaccagg gggaaaccct tgggatcttt atagtcctgt cgggtttcgc cgccactgat
      301 ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggcttcg
      361 ccgcagcctc atcgtttccc tgttaagttc cttcctggca tccaccagga aatttccacc
      421 tcacccgtaa gccggtaccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
      481 cgaggaagcg gcacatctcc cgttactaca tgttctgctg acgcgccggt gccgcctttt
      541 tctcctgcta catgaagcac ttcactgaca tccgcatccg tgccaacata ttaagccagt
      601 atacactccg ctagcgctac gtgactggtt cagggctgcg ccccgaaacc cgccaggacc
      661 actggcgcgc ctgacggctt gcccaacgct tcacggcacc tgattaaaat ccgctcccgt
      721 tcaccgtttc tgatggttct gacattccgc cgctgcttca ttgcttccac cttgtctggc
      781 agactggcca gcgtgatgct gaacaggaga cctacatgac gcgactgacc aatctgacgc
      841 cggcagagaa gcaatttctt gatgatgctg ttgcagctgc agagcgtgct tccggcaaga
      901 aactcaacca gccgaaccga catatcgtgc tcaaccgggc aagagcgcag attgagtcgc
      961 agcggcacgc cgaccggcag cgcgctctac gtgaggaaga acggcagcag gccgaattta
     1021 cctggtcacg tccgcgatcg ccgcgccggt gagtggctgt tatgaggttt ctgctgcagt
     1081 tttgtgatga aactgcaggc aaggcatttc cgtgcatagc catgcagacg cgcagaaatc
     1141 atcaggaacg tttttcgggg ttttccccgg ggaaaggtgc cggaaatcgg catgaggccg
     1201 cagaaacgcg ctgagagcgt cttaggcggt accgataagg gatgttatgg taaatgtgcc
     1261 tcacagactg gtacaaaaaa agccccgcag ggcttttttg ctatgagtta gccatatttt
     1321 cttgcgaatt tttcatcgga atcgcataga taaataataa actcaataaa cgcgatgatc
     1381 gcgggtatga atgtccagca aaaaattaga tacaggaatc cctgtcctat tttacctaag
     1441 taaaatttat gtgcgccaaa tccaccaagg aagaatgcta atagcgcggc tgcgattcta
     1501 cttttttcac ctgaacctga agaggcgttt gtggctccgc agtgtgggca tgactttgct
     1561 gtttcgtgta tttccttgcc gcatcctcgg cagtaaacca ttgaactcat tttttatcct
     1621 cgagcttatt tttagttggg atgcataata tccaatgttt tataacgata tgcatcctat
     1681 atgctcgggt gggatagatt ccattatggt gaaggatctt cttgagatcc tttctgtagc
     1741 gtcgtaatct cttgctctgg aaacgaaaaa accgccctgc aaggcggttt ttcgaaggtt
     1801 ctctgagcta ctaactcttt gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa
     1861 cttgtccttt cagtttagcc ttaaccggcg cataacttca agactaactc ctctaa
//
