LOCUS       NZ_CP011638             6393 bp    DNA     circular CON 15-JAN-2024
DEFINITION  Serratia marcescens strain CAV1492 plasmid pCAV1492-6393, complete
            sequence.
ACCESSION   NZ_CP011638
VERSION     NZ_CP011638.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03733805
            Assembly: GCF_001022215.1
KEYWORDS    RefSeq.
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Sheppard,A.E., Stoesser,N., Wilson,D., Sebra,R., Kasarskis,A.,
            Anson,L., Giess,A., Pankhurst,L., Vaughan,A., Grim,C.J., Cox,H.,
            Yeh,A., Sifri,C.D., Walker,S., Peto,T.E., Crook,D.W. and
            Mathers,A.J.
  TITLE     Rapid spread of a carbapenem resistance gene driven by multiple
            levels of genetic mobility
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Sheppard,A.E., Stoesser,N., Wilson,D., Sebra,R., Kasarskis,A.,
            Anson,L., Giess,A., Pankhurst,L., Vaughan,A., Grim,C.J., Cox,H.,
            Yeh,A., Sifri,C.D., Walker,S., Peto,T.E., Crook,D.W. and
            Mathers,A.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2015) University of Oxford, John Radcliffe
            Hospital, Oxford OX39DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP3 v. 2.2.0
            Genome Coverage                   :: 74X
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/15/2024 02:51:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,646
            CDSs (total)                      :: 5,521
            Genes (coding)                    :: 5,406
            CDSs (with protein)               :: 5,406
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 38 of 116
            Pseudo Genes (incomplete)         :: 82 of 116
            Pseudo Genes (internal stop)      :: 16 of 116
            Pseudo Genes (multiple problems)  :: 18 of 116
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP011638.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="CAV1492"
                     /isolation_source="Respiratory"
                     /host="Homo sapiens"
                     /db_xref="taxon:615"
                     /plasmid="pCAV1492-6393"
                     /geo_loc_name="USA:Virginia"
                     /lat_lon="38.14 N 78.45 W"
                     /collection_date="2011-12"
                     /collected_by="Clinical Microbiology University of Virginia
                     Health System"
     gene            complement(join(6077..6393,1..109))
                     /locus_tag="AB188_RS00020"
                     /old_locus_tag="AB188_00020"
     CDS             complement(join(6077..6393,1..109))
                     /locus_tag="AB188_RS00020"
                     /old_locus_tag="AB188_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676030.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="WP_015328245.1"
                     /translation="MGSYVDQSLTRNESVISRAQTSWIPTIIPVIIGILLLPFYGLGLL
                     IIIPVLLRVWSTELALTNQRVIAKVGLIRRNTVELRIDKVESLGIHQGILGRIFNYGSI
                     VIKGTGGTNTPIPNIKAPMQFRSIVNNHIEEMNSKQQ"
                     /name="PH domain-containing protein"
     gene            complement(1514..4411)
                     /locus_tag="AB188_RS00030"
                     /old_locus_tag="AB188_00030"
     CDS             complement(1514..4411)
                     /locus_tag="AB188_RS00030"
                     /old_locus_tag="AB188_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_004206881.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREILSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIVENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLADTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            4506..5111
                     /locus_tag="AB188_RS00035"
                     /old_locus_tag="AB188_00035"
     CDS             4506..5111
                     /locus_tag="AB188_RS00035"
                     /old_locus_tag="AB188_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            5459..5641
                     /locus_tag="AB188_RS29210"
     CDS             5459..5641
                     /locus_tag="AB188_RS29210"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_143350598.1"
                     /translation="MSSNAEWYIGHAVVELLVQERVVSMFSVIDILERRLQSGDSSKDE
                     FMEILEAIEKLLGYV"
                     /name="hypothetical protein"
     gene            complement(5624..5812)
                     /locus_tag="AB188_RS00040"
                     /old_locus_tag="AB188_00040"
     CDS             complement(5624..5812)
                     /locus_tag="AB188_RS00040"
                     /old_locus_tag="AB188_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_047727531.1"
                     /translation="MNRQESAALNMAKFIRAQTLLLLEKIDQLDLDDEATDCEKLHEQA
                     EKLYQKLQARLDSDITQ"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          1025..1197
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.266"
                     /coverage="81.0"
                     /evalue="1.6099999999999998e-54"
     Typing          complement(558..1042)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.206"
                     /coverage="93.0"
                     /evalue="3.54e-169"
ORIGIN
        1 gcaacaagat tccgataatt actgggatga ttgttggaat ccaggaggtt tgagcgcgag
       61 atattacgga ttcatttctc gtcaatgatt ggtctacgta gcttcccata aggttatatc
      121 catttagaaa aaataggatt tttccctttt taaggcgaat cagcaataga aactttatgt
      181 ctccgtggta ggtatacaaa atttacggag agcgtagcga ccgtcgcaca gttcggttct
      241 ttcctttcgt gccgcaactg gcttgattct gtgatggtgg tggtggctgc tgctatgtct
      301 catatactct tggcgttgca atcctttatc ccatatctgt cgaatatcac agatgatctt
      361 cttgagatcc tgtccgttgc gtcgtaatct cttgctctgg aaacgaaaaa accgccctgc
      421 aaggcggttt ttcgaaggtt ctcagagcta ccaactcttt gaaccgcggt aactggcttg
      481 gaggagcgca gtaaccaaat ctgtcctttc agtttagcct taacaggcgc ataacttcaa
      541 gactaactcc tctaaatccg ttaccaatgg ctaccgccac tggcgatttg tcgtgtcttt
      601 ccgggttgga ctcaagttga tagttaccgg aacgggcgtg gtagtcggac tgaacggggg
      661 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
      721 gaatgagata aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
      781 ggagagcgca cgagggagct accaggggga aacgcctggg atctttatag tcctgtcggg
      841 tttcgccgcc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta
      901 tggaaaaacg gcaacgccgc atccttttcc ccgtccttcc tgatttgagc gccttctctt
      961 gatcccgcta ttcgtgagcc ggtaacgccc gccgcagcct cacggcagga gcgcagcgac
     1021 cgagtgagcg aggaggcgtc atttcatcgc cacctgcatt tcgcacttac gcaccgcagc
     1081 cgcattttcc cctgccacat gaagcacttt cattaaatgt tactccgccg taacatattg
     1141 agccagtata cactccgcta gcgctatgtg actggttcag ggctgcgccc cgaaacctgc
     1201 gaagttccgt cttttgtgtt tcgcactgct aaactgtcaa tattttcttt ctgctgccgt
     1261 taactgatgt ttacttgttt ggtttttcct ctggaatcct tatgaatatg agtgatgctg
     1321 aacggcagct ggttgtttct gttatttctg actgcttacg ccagtccggg ggagactccg
     1381 gtaagatgat gctggatgct tattatctgg ccgtatccga gacagatccc tgtgtcaggg
     1441 agtgcatgct ggcgctcctt gaatcagttc gtattaaata ggggtcggtt ccggctgagg
     1501 gcgaaatgac accctaagct ttcggttcct tgggccaaag atattcgcca gtcagtagaa
     1561 tgtgcgccca gcccaatggg gatatgtggg gaagaaattc agggggaaca tccaaccctt
     1621 cgttccgccg ctccgtgacg gcatgaccaa gatggacggt attccagtaa atgatcaccg
     1681 cagtcaataa attgagccca gcgattcggt agtgctgccc ctctgtcgtg cgatcgcgaa
     1741 tttccccctg cctcccgata cggagcgcat ttttgagcgc atggtgggcc tctcccttgt
     1801 taagaccgat ctgagcacgc cgctgcatgt ccgtatccag gatccactca ataatgaaaa
     1861 gggtccgttc aatacgacca acttcacgaa gcgcaactgc aaggttgttt tgtcgtgggt
     1921 aagaagcgag cttgcgcagg agttggctgg gcctgatttt gccagcggtc atcgtcgcgg
     1981 cacaacggaa aatatcaggc cagttcgcaa cgataagatc ctcccgggct tttccaccta
     2041 ccaacttgcg taactccctg ggggtcgtat cgggattaaa tacgtacaac cgcttcgatg
     2101 gcagatccct gattcgcaga acgagattgt agccgagcag gctactggct ccgaacaaat
     2161 ggtcggtgaa tcctgctgta tcggcatact gttcgcgaac atggcgaccg acctcgttca
     2221 tcagtagtcc atcgagaata tacggtgcct cgctcacggt cgccgggatc gactgacaag
     2281 cgaatggcgc gaactggtcg cttacgtgag tatacgcttt gaggccggga acagaaccat
     2341 atttggcatt gaccatgttc atggcttcgc catgccgcgc tgtcgggaaa aactgaccat
     2401 cgctcgatgc tgacgtgccc atcccccaga cgcgtgacat cggcagttta ccctgcgcgg
     2461 ccaccacaat tgccaatgcc tggttcatgg cttcgctttc aacatgccag cgggcaaggc
     2521 gtgagagctg ccagtaatca tgcgtgtttg tagcttccgc catcttacgc aggcccagat
     2581 tgagcccttc agcgagcagg acgttgagca gaccgatccg gtcgcgacat ggagccccgg
     2641 ttctcagatg ggtaaacgca tctgtgaaac caagggctgc atcaacttca agcagcatgt
     2701 cggtaatccg aacggacggc attcggcgat acagatccag tatgagtgcc tcggcaccat
     2761 ccggcacgtc tgctgtcaac ctgtcgatcc gcaacgttcc atcttctatg ctaccgtgcg
     2821 gaatagtgcc gttacgggca gcccgggcca gccgcttaag agcgatcgtg agtcgcgcct
     2881 ttctgtctgc cagccaatcc tgtgggttgg aaggcacggc cagttttgca ttttcctgcg
     2941 ccgcgatcat cggcaccagt acctgcttga ggtcaccata gcggcgcgaa tgagcgagcc
     3001 agacatctcc ggaacgaaaa gcatcccgga ggtgaaagag taccgccact tcccaaagac
     3061 gggtatctcc tttttcctga gctcgtaaat gacggttcca tttggagctg ggccgcagga
     3121 aacgcctttc tggcgatgca acacctttca tctctccgat cgacaaagct gctgctacca
     3181 atggtccggc gaccggcgcg gcttcgagct tcagacagcg caacatgcgg ggcgcataac
     3241 gacgaaagcg atggtatccc tgcccgacat atgcaagagg ctcatcggct agcgtgttgc
     3301 tgagttgagt ccctgtcgct accagttgag cgagccggtc ccatgcaacc gaactggcga
     3361 cagccatctc cagcggggtt ccgtcactgc gggcctcaag caacgaagct cccagcgcgg
     3421 tgaaggtacg gatcgtatcc gcgagtgtgg ctttagagcc ggaaattgtt tcgtcatgct
     3481 ggcgcttcgc ttcccgccag gtttttccta cgatcctgtc atgggtttcg actatggcat
     3541 cagcaatcgc cgcttcccac tccacaacac agacggcaag gatcgcccag cggcggtccg
     3601 aagtgatgtc acgcaaaccg tcggtgaagt agcgttcacc ctgccgacgc agccgggcaa
     3661 tgcgatgggc aggtatgctg gccaaagcac tatgattgat attcagggta cgcagaaatt
     3721 cgagcctgtc gagcaaacgg ttagcagcag ccgagttgtt accaacctcg aagttgcgaa
     3781 gccagatgaa acgactgata ttgccggcga gcatttcact cagaagtttg tccaggtgat
     3841 cgcgaacatc cgctgttaaa ttttccacaa tccgcgtttc aatccgccgc tcagcggcga
     3901 ccagagcatc cgcgcacaag cgctcgattg tcgatactgc gggcagaatg gtggaagttt
     3961 cccgacaccg cacaataaaa cgatgagcaa gatcctcgtt tgatctggca tcttcggcct
     4021 ggccgaaagt ccactcccgc agatcacggg caccacggcc cgtgaaggtc ttgtagccgt
     4081 aaatttcgcg cagcgtgtcc atgtgctgct gacgggtttg gcgccgtgtg gcataagtga
     4141 gaagcgcatc agccggaact ccaagctgag caccgacgaa ggaaaggatt tcacgcggga
     4201 tcatctcacc aggagccagt gcacggcccg gatatcgtaa ggcacaaagt tgcagggcaa
     4261 agccaatcct gttttccggt ctgcggcgct gcctaatgtt ttccaggtca tcatcgccca
     4321 gcgtgtagaa cttcagtagc gacagttcgt ccgtgggcag atcgaacagc gctgctcgct
     4381 gccgttcggt gaaaatatgg cgtcgtgaca tacaaattcg tcccttttga agtatagtct
     4441 gttttggaca acagccagcc catataaatc agggcgttcc gatacaaaaa tccaggaggg
     4501 ttcaattggg acatcgtgcc gccatttact gccgggtttc aacagcggat cagtcttgtg
     4561 aacgccagga atttgatctg cgagccttcg ccggccgtgc cggctacgac gtggtgggaa
     4621 tatttaagga aacaggttca ggaactaaac tcgaccgggc cgagcgaaag aaagtcctgg
     4681 cgcttgccca gtccagacaa attgatgcaa tcctggtcac tgagctttcc cggtgggggc
     4741 gctcgacgct cgatctgctc aatacgctac gtgaactgga gaactggaag gtttccgtga
     4801 tagccatgaa tggaatggcg ttcgatcttt cgtcgccgta tggacgaatg ctggcgacgt
     4861 ttctttccgg cattgcggag tttgagcggg atctcatcag cgagcgggtc aagtcaggcc
     4921 ttgctgttgc gaaggcacgt ggtaagaggc ttggtcgtca ggccggagtg cgaccaaaat
     4981 cagaccgact tttgcctaag gtggttgcga tgagggccga gggacgcagc tatcgctgga
     5041 tcgcacgcga gctcggtatc agcaagaata ccgtcgctga catcgtgcaa cgacacagag
     5101 ctaacgctta gggtgtcatt tcgccctcag ccggaaccga ccccttaaat atgtcaccgg
     5161 ctgagtgttt tctgattttt gaaccatact ctttgtcatg cggccgtcat ggatgatggt
     5221 cgttgtaaca tgcactatgt aactgaagag tgttctaatc tagcttgact taccgggctt
     5281 accgcccggt tttttttccg ttcttctgac tgtactccag agcaggtttt gccttttttc
     5341 tactgcgctt tcggagtatg actaattttc tgtcagggct gcgccccgac accccgcaag
     5401 cccttcacgg actcgcaata aatttcgtgt ccgttcaccg cttccagtat gagttgccgt
     5461 gagttcaaat gctgaatggt atatcggtca tgctgtggtt gagttgcttg tacaggagcg
     5521 tgttgtctcg atgttctctg ttattgatat ccttgagcga cgtctgcaga gcggtgattc
     5581 ttcaaaagac gagtttatgg agattctgga ggcgatcgaa aagctactgg gttatgtctg
     5641 agtccagtct tgcctggagt ttctgataaa gtttttctgc ctgttcgtgt aatttttcac
     5701 agtctgtcgc ttcgtcatca aggtcgagct ggtcgatttt ttccagcaaa agcagcgtct
     5761 gggcgcggat aaatttagcc atgttgagag cggcagattc ctgacggttc acgtgtgttt
     5821 ttccctgtct ggttatcatt tctaagtgaa aacagaattc cgtcattctg gtgaatagtc
     5881 atgcatggcg ttaaaattta tcaggcgcgt ttctagcggg ttttctggtg gtttgttgcc
     5941 tgttttaccg gtttcccgtc agaaacgccc tgagagcctc tcaggcggtg cacgcaacgg
     6001 gtgttatggt aaatccagct gattggcagg tacaaaaaaa gccctacggg gctttttttg
     6061 taatgctacg aggaagctac tgctgcttag aattcatttc ctcgatatga ttgttcacaa
     6121 tcgatctgaa ttgcattggc gccttgatgt ttggaattgg agtgttggtt ccgccagtgc
     6181 ctttaatcac gatagaccca tagttaaaga ttcgtcccag tatcccttga tgtatgccaa
     6241 ggctttcaac tttatcaata cggagttcca ccgtattgcg tcggatcaaa cccacttttg
     6301 cgatgactct ttggttagtt aatgcaagct ccgttgacca aactctaagg agcacaggga
     6361 ttattataag taaccctaag ccgtaaaaag gca
//
