LOCUS       NZ_CP011637             3223 bp    DNA     circular CON 15-JAN-2024
DEFINITION  Serratia marcescens strain CAV1492 plasmid pCAV1492-3223, complete
            sequence.
ACCESSION   NZ_CP011637
VERSION     NZ_CP011637.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03733805
            Assembly: GCF_001022215.1
KEYWORDS    RefSeq.
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Sheppard,A.E., Stoesser,N., Wilson,D., Sebra,R., Kasarskis,A.,
            Anson,L., Giess,A., Pankhurst,L., Vaughan,A., Grim,C.J., Cox,H.,
            Yeh,A., Sifri,C.D., Walker,S., Peto,T.E., Crook,D.W. and
            Mathers,A.J.
  TITLE     Rapid spread of a carbapenem resistance gene driven by multiple
            levels of genetic mobility
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Sheppard,A.E., Stoesser,N., Wilson,D., Sebra,R., Kasarskis,A.,
            Anson,L., Giess,A., Pankhurst,L., Vaughan,A., Grim,C.J., Cox,H.,
            Yeh,A., Sifri,C.D., Walker,S., Peto,T.E., Crook,D.W. and
            Mathers,A.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2015) University of Oxford, John Radcliffe
            Hospital, Oxford OX39DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP3 v. 2.2.0
            Genome Coverage                   :: 74X
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/15/2024 02:51:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,646
            CDSs (total)                      :: 5,521
            Genes (coding)                    :: 5,406
            CDSs (with protein)               :: 5,406
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 38 of 116
            Pseudo Genes (incomplete)         :: 82 of 116
            Pseudo Genes (internal stop)      :: 16 of 116
            Pseudo Genes (multiple problems)  :: 18 of 116
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP011637.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="CAV1492"
                     /isolation_source="Respiratory"
                     /host="Homo sapiens"
                     /db_xref="taxon:615"
                     /plasmid="pCAV1492-3223"
                     /geo_loc_name="USA:Virginia"
                     /lat_lon="38.14 N 78.45 W"
                     /collection_date="2011-12"
                     /collected_by="Clinical Microbiology University of Virginia
                     Health System"
     gene            join(3202..3223,1..311)
                     /locus_tag="AB188_RS27550"
     CDS             join(3202..3223,1..311)
                     /locus_tag="AB188_RS27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            1329..1652
                     /locus_tag="AB188_RS00005"
                     /old_locus_tag="AB188_00005"
     CDS             1329..1652
                     /locus_tag="AB188_RS00005"
                     /old_locus_tag="AB188_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1861..>2295
                     /locus_tag="AB188_RS29845"
                     /pseudo=""
     CDS             1861..>2295
                     /locus_tag="AB188_RS29845"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2330..2815
                     /locus_tag="AB188_RS29200"
                     /old_locus_tag="AB188_00010"
     CDS             2330..2815
                     /locus_tag="AB188_RS29200"
                     /old_locus_tag="AB188_00010"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            2819..3034
                     /locus_tag="AB188_RS29205"
                     /old_locus_tag="AB188_00015"
     CDS             2819..3034
                     /locus_tag="AB188_RS29205"
                     /old_locus_tag="AB188_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     ARG             4..311
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.84"
                     /coverage="95.33"
     Typing          1042..1233
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          complement(579..1059)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.867"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          1643..3132
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaggggctat tgtggctgaa gtcataggca ccacctttat gaaatattca gaaggattta
       61 cacgcctgtg gccgtctgta ggaactgcaa taagttattg cgctgccttt tatctgcttt
      121 cccaaacatt ggaaaatata cctaccggta ttgcttacgc aatttggtct ggtgcgggaa
      181 ttgtactaat aagcctagct ggttggttgg ttagcggtca aaaacttgat tggcccgcaa
      241 ttgcaggaat gttccttatt tgcgttggag ttttggttat taatattttt tcaaaatctt
      301 ctgcgcatta actttttacc gaagcatatt ggccaaagca aaaggatttc cttgagatcc
      361 tttctttctg cgcgtaatct ttttccctgt aaacaaaaaa accaccgcta ccaacggtgg
      421 ttcgtttgcc ggaattcaga gctaccaact ctttgttccg atggtaactg gctttcacgg
      481 agcgcagata cctaaactgt cctttcagtg tagccgcact caggctttca cttcaagaac
      541 tctgtaggca ttctctacaa tctctgctcc gtgaacccag ttaccaatgg ctaccgccac
      601 tggcgatttg tcgtgtcttt ccgggttgga ctcaagttga tagttaccgg aacgggcgtg
      661 gtagtcggac tgaacggggg gttcgtgcat acagtccatc ctggagcgaa ctgcctaccc
      721 ggaaccgagt ttcaggcgtg gaatgagaaa ccgcggccat aacagcggaa tgacaccggt
      781 aaaccgaaag gcaggaatgc gggggagcac gagggagcca ccagggggaa acgcctggta
      841 tctttaagcc gcatcgggtt tcgccaccgc tgatttgagc gtcattttcg tgatactcgt
      901 caggggggcg gagcctatgg aaaaacggca actccgcatc cttttcttgg cctttcctgc
      961 ttcgagcggc atttgcttat cccgtaatct gtgagctggt aacgcccgcc gcagtctcac
     1021 ggcaggagcg tagcgaccga gtgagcgagg aggcgtcatt tcatcaccac ctgcattttg
     1081 cacttacgcc cggcagccgc atttttcccc tgtcacatga agcactttca taaattgcca
     1141 ctccgtcata acatgtttag ccagtataca ctccgctagc gctacgtgac tggttcaggg
     1201 ctgcgccccg aaacccgcta aaaccactgc cgcgcctgcg gcttgtccaa caccgtgccg
     1261 accgggaaaa gatttcccgt ccgtcccggc gttatcagga ggccggattg gcagacaaac
     1321 gcagcaaaat gctcaccatg tgggtgactg aggacgagca ccggcgtctg ctggagcgct
     1381 gcgagggtaa gcaactcgcg gcctggatgc ggcagacgtg cctggacgag aagcccgcac
     1441 gcgccggcaa acttccgtcg atctcgccgg cgctgcttcg tcagcttgcc ggcatgggga
     1501 acaaccttaa ccagattgcc cgccaggtta acgccggtgg tggaagcgga cacgaccgcg
     1561 tgcagattgt cgccgcgctg atggccatcg atgccggact cgagcggttg cggcatgccg
     1621 tactggaaaa gggtgctgat gatgatcgtt aagtttcatc ctcgggggcg cggcggcggc
     1681 ggcgggccgg tcgattatct gctgggtaaa gatcgccagc gcgacggcgc cagcgttctg
     1741 caggggaagc cggatgaagt ccgggagctt atcgacgcct cgccctacgc caaaaagtac
     1801 acttccggcg ttctgtcttt tgccgaacag gatttaccgc ccggccagcg cgaaaagctg
     1861 atggcgagtt tcgagcgggt tctgatgccc ggactcgata aagaccagta cagcgtgctg
     1921 tgggttgagc accgggacaa ggggcggctg gagctgaact tcctgatccc gaacacggag
     1981 cttctgaccg gcaagcggct acagccgtat tacgaccgcg ccgaccgtcc gcgcatcgat
     2041 gcctggcaga ccatagtgaa cggcaggctg gggctgcatg acccgaacgc gccggagaac
     2101 cggcgggtgc tggtctcgcc ttccgcgctg ccggaagcga agcaggaagc cgcccaggcg
     2161 attacgagcg gcctgcttgc ccttgcctca tccggggagc tgaaaacgcg tcaggacgtc
     2221 actgaggcgc tggaaagcgc aggttttgag gtggtgcgca ccacaaaaag cagcatcagc
     2281 attgccgacc cggacggggg gcgaaacatc cgactgaagg gagccatcta tgaacagtct
     2341 tttaacgctg gcgaaggact tagagcagaa atcgaaagcg cagcagcaga ctaccggcga
     2401 gatgctgaaa gccgcattca gcgagcacga gaagtctgtc agagcggaac tgagcgaaag
     2461 cgagaagaga atcagcgccg ccatcctcga ccacgaccgg aagctgtcct cagccatgag
     2521 ccagcgcacg aaagggatgc tgcgcatggt cagccagacg tggctgacca tcgttctggt
     2581 ctccgcgctg ctgatagcgt cgagcgcggg cattctgtgg tggcaggggc agcagatact
     2641 cgagaactat acgaccatcc gggagcagaa gagcacgcag gccatgctgt cagagaggaa
     2701 cagcggcgta cagctctcga cctgcggcga gcagagacgc cgctgcgtga gggtgaaccc
     2761 ggaagcggga cagttcggag aggactcgag ctggatgata ctggcgggga aatagcacat
     2821 gacggagctg gaaaaacagt tgctgagcgc attagagcag ctacagcagg actactcgaa
     2881 aaggctggac gagtgggaga acgccttcgc ggaatggcgg acgatgtctg gtcttattca
     2941 acgggagaac gcggcgctga acgagcgcgt cacggtcttg agcaggcagg tgcagagttt
     3001 gagcgagcag ctgcgccggt tgtcgaaagg ctgaacgcga tagaaactca ccggcagcag
     3061 gagcgggcgg tgcagcacca gaaagcgctg gagctggaac gatcgcagtg gcagcatcat
     3121 ggtccttcgc tatgataagc cgggttctat taattagttg ttatgtaaag atatttaaat
     3181 ttttcaacat gaggtatttt tatgaataca tatattttcc tgg
//
