LOCUS       NZ_CP011586            10403 bp    DNA     circular CON 01-FEB-2024
DEFINITION  Enterobacter asburiae strain CAV1043 plasmid pCAV1043-10, complete
            sequence.
ACCESSION   NZ_CP011586
VERSION     NZ_CP011586.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03733641
            Assembly: GCF_001022095.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 10403)
  AUTHORS   Sheppard,A.E., Stoesser,N., Wilson,D., Sebra,R., Kasarskis,A.,
            Anson,L., Giess,A., Pankhurst,L., Vaughan,A., Grim,C.J., Cox,H.,
            Yeh,A., Sifri,C.D., Walker,S., Peto,T.E., Crook,D.W. and
            Mathers,A.J.
  TITLE     Rapid spread of a carbapenem resistance gene driven by multiple
            levels of genetic mobility
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10403)
  AUTHORS   Sheppard,A.E., Stoesser,N., Wilson,D., Sebra,R., Kasarskis,A.,
            Anson,L., Giess,A., Pankhurst,L., Vaughan,A., Grim,C.J., Cox,H.,
            Yeh,A., Sifri,C.D., Walker,S., Peto,T.E., Crook,D.W. and
            Mathers,A.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2015) University of Oxford, John Radcliffe
            Hospital, Oxford OX39DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP3 v. 2.2.0
            Genome Coverage                   :: 101X
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/01/2024 03:07:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,932
            CDSs (total)                      :: 4,817
            Genes (coding)                    :: 4,660
            CDSs (with protein)               :: 4,660
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 31 of 157
            Pseudo Genes (incomplete)         :: 121 of 157
            Pseudo Genes (internal stop)      :: 17 of 157
            Pseudo Genes (multiple problems)  :: 12 of 157
            Pseudo Genes (short protein)      :: 1 of 157
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP011586.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10403
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="CAV1043"
                     /host="Homo sapiens"
                     /db_xref="taxon:61645"
                     /plasmid="pCAV1043-10"
                     /geo_loc_name="USA:Virginia"
                     /lat_lon="38.14 N 78.45 W"
                     /collection_date="2008-03"
                     /collected_by="Clinical Microbiology University of Virginia
                     Health System"
     gene            complement(join(10032..10403,1..51))
                     /locus_tag="AB190_RS00015"
                     /old_locus_tag="AB190_00015"
     CDS             complement(join(10032..10403,1..51))
                     /locus_tag="AB190_RS00015"
                     /old_locus_tag="AB190_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004216558.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKGIKVID
                     TSELIRVFGSLDGVIDAVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(51..242)
                     /locus_tag="AB190_RS00020"
                     /old_locus_tag="AB190_00020"
     CDS             complement(51..242)
                     /locus_tag="AB190_RS00020"
                     /old_locus_tag="AB190_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032421723.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(723..872)
                     /locus_tag="AB190_RS26415"
     CDS             complement(723..872)
                     /locus_tag="AB190_RS26415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_017880354.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1434..1565
                     /locus_tag="AB190_RS26515"
                     /old_locus_tag="AB190_00025"
     CDS             1434..1565
                     /locus_tag="AB190_RS26515"
                     /old_locus_tag="AB190_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047741455.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGKQDVREM"
                     /name="hypothetical protein"
     gene            complement(2192..>2416)
                     /locus_tag="AB190_RS26075"
                     /pseudo=""
     CDS             complement(2192..>2416)
                     /locus_tag="AB190_RS26075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /name="IS110 family transposase"
     gene            complement(2450..5347)
                     /locus_tag="AB190_RS00035"
                     /old_locus_tag="AB190_00030"
     CDS             complement(2450..5347)
                     /locus_tag="AB190_RS00035"
                     /old_locus_tag="AB190_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_001553819.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            5442..6047
                     /locus_tag="AB190_RS00040"
                     /old_locus_tag="AB190_00035"
     CDS             5442..6047
                     /locus_tag="AB190_RS00040"
                     /old_locus_tag="AB190_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            complement(<6077..6865)
                     /locus_tag="AB190_RS00045"
                     /old_locus_tag="AB190_00040"
                     /pseudo=""
     CDS             complement(<6077..6865)
                     /locus_tag="AB190_RS00045"
                     /old_locus_tag="AB190_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011145548.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS4321 family transposase"
                     /name="IS110-like element IS4321 family transposase"
     gene            complement(7296..7508)
                     /gene="cspG"
                     /locus_tag="AB190_RS00050"
                     /old_locus_tag="AB190_00045"
     CDS             complement(7296..7508)
                     /gene="cspG"
                     /locus_tag="AB190_RS00050"
                     /old_locus_tag="AB190_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="WP_001548019.1"
                     /translation="MSNKMTGLVKWFNSDKGFGFITPADGSKDVFVHFSAIQSNDYKTL
                     DEGQKVEFSIENGAKGPAAGNVVGL"
                     /name="cspG"
     gene            complement(7896..8593)
                     /locus_tag="AB190_RS00055"
                     /old_locus_tag="AB190_00050"
     CDS             complement(join(7896..8344,8344..8593))
                     /locus_tag="AB190_RS00055"
                     /old_locus_tag="AB190_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_230683675.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDQVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(8674..9210)
                     /locus_tag="AB190_RS00060"
                     /old_locus_tag="AB190_00055"
     CDS             complement(8674..9210)
                     /locus_tag="AB190_RS00060"
                     /old_locus_tag="AB190_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002069891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047741458.1"
                     /translation="MKKVYKAIILIVLLCGGVLSANYIFLQRHMNEVLKEDPRNDGISV
                     WVYYKWFVISSEINYDLRSVSAENSSLDVNRVMLQFAEKVKDDDFSKVYLSYRGKDKFY
                     LKGGYFKTLGQEYGIQNPVYTLRTIPENVYMLNGERAYSVWEGGLLGVMGKQMEDLSDF
                     SKAWYLDDFIKSMSD"
                     /name="hypothetical protein"
     Typing          1028..1543
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.871"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gcgactaacc ttaacaagcc gtgctgcttc actaatagac actctggcca tcagttacca
       61 tcctgtaatt catccagtct cgaggacagt gtacggaaaa gatgctccgc atcatcgtgg
      121 agtttctcac aaaggtcggc ctcagcatcg agatcaagct cgttaagctt ctccagcagg
      181 agcagagact gattctggat gaatttagcc atgttcagcg ctgttttctg ttgtttcgtc
      241 ataagggacc tcagagcatc aatgctcagt gtaaaaccgt taagggggtc ggatgtacga
      301 atggttcaga gaacggatct gaggtaatcc tgtagttatt ttgtagttta tctgtagtca
      361 tcgcactaaa aatcaggatt aagtcatcag cttttgtagt ctttctgtag tttacctgta
      421 gtccggaaaa ataatccgtt catgcgagca tgaacgaggt cgttaatgac gtttttttgt
      481 caccgacgcc ccttaacctc cgcagatcac ccggccagaa tgataatttt ttggccctta
      541 aatggtcata cacctaacgc agccgcgatt ttcatcgcaa ctgcatgaat taaaaagtaa
      601 aaactgaaat ctccgtttat ttatgtaata cgcaacgtat tacaatcacc tgtaattaca
      661 tgaaaataaa gaaataaaat gtaataccgg cgtattacca cgtacgcggg caagcgaaaa
      721 agctataacg cttcccggcc gacaggcggc ccggaatcca caggggcatg caggttctct
      781 tcagtaacct gcgccattaa atcaaaaagg tcatattcgc cgggaagctg cgtatcagtc
      841 gaatccagtt gttcatcata ttcagatggc atggggtgat atccagtagc tgagaggttg
      901 ttagcggggt tctgggcgca gccctgaacc agtcgagtag cactagctga gtgtatacgg
      961 gctcaatatg ctacggtgga gtaacacgtt atgaaagtgc ttcatgtgac agaggaaagt
     1021 gcagcggcgg ggcgtacggg agaaaatgga agcccgggga tatgacgcct cctcgctcac
     1081 tcggtcgcta cgctccggcc gttcggctgc ggcgggcgtt acctgctcac gaatgacgag
     1141 gtaacgagat gaagctgagg tcaagaaagg ccggaaaaag gatgcggcat tgccgttttt
     1201 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg
     1261 aaacccgaca ggactataaa gataccaggc gtttccccct ggtggctccc tcgtgcgctc
     1321 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttatctca
     1381 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg
     1441 aaccccccgt tcagtccgac tgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1501 cggaaagaca tgcaaaagcg ccactggcag cagccactgg taaacaggat gtgcgagaga
     1561 tgtagtaaga gttcttgaag tgatggcctg agtgcggcta cactgaaagg acagatttgg
     1621 tgacatgtct cgcacaagac tgttaccgcg gttagaaaat ctgccaggat taactaacct
     1681 tcgaaaaacc acctgccagg gtggtttttt cgtttacaga gcaagagatt acgacgcaac
     1741 agaaaggatc tcaagaagtt ccttgctgta ttgctactat cctagagttc tgacccatta
     1801 ggggcattat cagatgacgg ccttgtaatc cacggggagc acgtcactta tactgtacaa
     1861 aagtatgaag tagtgcaaaa tgcatttggt tttatagtat tatgatgcgt atagggggtc
     1921 cgcttggaaa acggaatcta tggtcactcc cgtttttgca acaccgattt tgacgacaag
     1981 ttggcttgct tgaatctatc cggcgtctga atgggatttt attcccgcgc cttgatgagt
     2041 tccgcgcctg atgaacctcc agaaaatata cggcttcaat gagcctttcc gttttacagg
     2101 ttcctcaaca ggccggtggg ccgttagtat catcaatatc agtattcgca aaaccagatc
     2161 agtaattctt taaaccggtg tatttctgcc gttatgctac ataagtttgc tgtcgtgccg
     2221 ttagggccca ggctattctg gccagcttgt ttgccagagc acaagtgacg acaaagttgc
     2281 ttttccggca cagtaaatcc ctgacccaat cggccaattt gccagactgg tgttccagtt
     2341 tttgtatgaa taccctggca cattgaacca acaaagttcg gatcttttta ttacctcgct
     2401 tactaattcc cagcaagggg tcggttccgg ctgagggcga aatgacaccc taagctttcg
     2461 gttccttggg ccaaagatat tcgccagtca gtagaatgtg cgcccagccc aatggggata
     2521 tgtggggaag aaattcaggg ggaacatcca acccttcgtt ccgccgctcc gtgacggcat
     2581 gaccaagatg gacggtattc cagtaaatga tcaccgcagt caataaattg agcccagcga
     2641 ttcggtagtg ctgcccctct gtcgtgcgat cgcgaatttc cccctgcctc ccgatacgga
     2701 gcgcattttt gagcgcatgg tgggcctctc ccttgttaag accgatctga gcacgccgct
     2761 gcatgtccgt atccaggatc cactcaataa tgaaaagggt ccgttcaata cgaccaactt
     2821 cacgaagcgc aactgcaagg ttgttttgtc gtgggtaaga agcgagcttg cgcaggagtt
     2881 ggctgggcct gattttgcca gcggtcatcg tcgcggcaca acggaaaata tcaggccagt
     2941 tcgcaacgat aagatcctcc cgggcttttc cacctaccaa cttgcgtaac tccctggggg
     3001 tcgtatcggg attaaatacg tacaaccgct tcgatggcag atccctgatt cgcagaacga
     3061 gattgtagcc gagcaggcta ctggctccga acaaatggtc ggtgaatcct gctgtatcgg
     3121 catactgttc gcgaacatgg cgaccgacct cgttcatcag tagtccatcg agaatatacg
     3181 gtgcctcgct cacggtcgcc gggatcgact gacaagcgaa tggcgcgaac tggtcgctta
     3241 cgtgagtata cgctttgagg ccgggaacag aaccatattt ggcattgacc atgttcatgg
     3301 cttcgccatg ccgcgctgtc gggaaaaact gaccatcgct cgatgctgac gtgcccatcc
     3361 cccagacgcg tgacatcggc agtttaccct gcgcggccac cacaattgcc aatgcctggt
     3421 tcatggcttc gctttcaaca tgccagcggg caaggcgtga gagctgccag taatcatgcg
     3481 tgtttgtagc ttccgccatc ttacgcaggc ccagattgag cccttcagcg agcaggacgt
     3541 tgagcagacc gatccggtcg cgacatggag ccccggttct cagatgggta aacgcatctg
     3601 tgaaaccaag ggctgcatca acttcaagca gcatgtcggt aatccgaacg gacggcattc
     3661 ggcgatacag atccagtatg agtgcctcgg caccatccgg cacgtctgct gtcaacctgt
     3721 cgatccgcaa cgttccatct tctatgctac cgtgcggaat agtgccgtta cgggcagccc
     3781 gggccagccg cttaagagcg atcgtgagtc gcgcctttct gtctgccagc caatcctgtg
     3841 ggttggaagg cacggccagt tttgcatttt cctgcgccgc gatcatcggc accagtacct
     3901 gcttgaggtc accatagcgg cgcgaatgag cgagccagac atctccggaa cgaaaagcat
     3961 cccggaggtg aaagagtacc gccacttccc aaagacgggt atctcctttt tcctgagctc
     4021 gtaaatgacg gttccatttg gagctgggcc gcaggaaacg cctttctggc gatgcaacac
     4081 ctttcatctc tccgatcgac aaagctgctg ctaccaatgg tccggcgacc ggcgcggctt
     4141 cgagcttcag acagcgcaac atgcggggcg cataacgacg aaagcgatgg tatccctgcc
     4201 cgacatatgc aagaggctca tcggctagcg tgttgctgag ttgagtccct gtcgctacca
     4261 gttgagcgag ccggtcccat gcaaccgaac tggcgacagc catctccagc ggggttccgt
     4321 cactgcgggc ctcaagcaac gaagctccca gcgcggtgaa ggtacggatc gtatccgtga
     4381 gtgtggcttt agagccggaa attgtttcgt catgctggcg cttcgcttcc cgccaggttt
     4441 ttcctacgat cctgtcatgg gtttcgacta tggcatcagc aatcgccgct tcccactcca
     4501 caacacagac ggcaaggatc gcccagcggc ggtccgaagt gatgtcacgc aaaccgtcgg
     4561 tgaagtagcg ttcaccctgc cgacgcagcc gggcaatgcg atgggcaggt atgctggcca
     4621 aagcactatg attgatattc agggtacgca gaaattcgag cctgtcgagc aaacggttag
     4681 cagcagccga gttgttacca acctcgaagt tgcgaagcca gatgaaacga ctgatattgc
     4741 cggcgagcat ttcactcaga agtttgtcca ggtgatcgcg aacatccgct gttaaatttt
     4801 ccgcaatccg cgtttcaatc cgccgctcag cggcgaccag agcatccgcg cacaagcgct
     4861 cgattgtcga tactgcgggc agaatggtgg aagtttcccg acaccgcaca ataaaacgat
     4921 gagcaagatc ctcgtttgat ctggcatctt cggcctggcc gaaagtccac tcccgcagat
     4981 cacgggcacc acggcccgtg aaggtcttgt agccgtaaat ttcgcgcagc gtgtccatgt
     5041 gctgctgacg ggtttggcgc cgtgtggcat aagtgagaag cgcatcagcc ggaactccaa
     5101 gctgagcacc gacgaaggaa aggacttcac gcgggatcat ctcaccagga gccagtgcac
     5161 ggcccggata tcgtaaggca caaagttgca gggcaaagcc aatcctgttt tccggtctgc
     5221 ggcgctgcct aatgttttcc aggtcatcat cgcccagcgt gtagaacttc agtagcgaca
     5281 gttcgtccgt gggcagatcg aacagcgctg ctcgctgccg ttcggtgaaa atatggcgtc
     5341 gtgacataca aattcgtccc ttttgaagta tagtctgttt tggacaacag ccagcccata
     5401 taaatcaggg cgttccgata caaaaatcca ggagggttca attgggacat cgtgccgcca
     5461 tttactgccg ggtttcaaca gcggatcagt cttgtgaacg ccaggaattt gatctgcgag
     5521 ccttcgccgg ccgtgccggc tacgacgtgg tgggaatatt taaggaaaca ggttcaggaa
     5581 ctaaactcga ccgggccgag cgaaagaaag tcctggcgct tgcccagtcc agacaaattg
     5641 atgcaatcct ggtcactgag ctttcccggt gggggcgctc gacgctcgat ctgctcaata
     5701 cgctacgtga actggagaac tggaaggttt ccgtgatagc catgaatgga atggcgttcg
     5761 atctttcgtc gccgtatgga cgaatgctgg cgacgtttct ttccggcatt gcggagtttg
     5821 agcgggatct catcagcgag cgggtcaagt caggccttgc tgttgcgaag gcacgtggta
     5881 agaggcttgg tcgtcaggcc ggagtgcgac caaaatcaga ccgacttttg cctaaggtgg
     5941 ttgcgatgag ggccgaggga cgcagctatc gctggatcgc acgcgagctc ggtatcagca
     6001 agaataccgt cgctgacatc gtgcaacgac acagagctaa cgcttagggt gtcatttcgc
     6061 cctcagccgg aaccgacccc agcaatgtcg tcctacctcc cgtgctgtac tgccgaggta
     6121 caagccctgt tgccgccgca aagtcacggc tgctggcgta ctgcttcccg tcgccaatct
     6181 cagttgaaat agtactcgct gtcagtgttc cgacgcaggg aatgctcagc aagcgctgtc
     6241 caacctcatc ttcgtccaac tttcgtttca actgggattc caaatcttta atctgctcaa
     6301 caagatagtg ataatgctgt tgtaatttca gcaataactg gctgaggtaa agaggcaaac
     6361 tattatcctc aagaatggta ctcagtcggc taataacggc agctcctcgg ggaacgctaa
     6421 tgccaaattc cagcagaaaa gcatgcattt gattggttgt ttttacctta tcctgaacca
     6481 gggattcacg gacacgatgc agagcccgca ttgcctgctg agattccgtt ctgggctgca
     6541 caaaacgcat agacggacgc gatgcagctt cacaaatagc ttcggcgtcg acaaagtcgt
     6601 ttttattgct tttaacgaac gggcggacaa attgtggtga tatcagcttt ggggaatgcc
     6661 ccaactcttc caacttgcgt gccataaagt gagaaccgcc acaggcttcc attgcgatgg
     6721 ttgtagcggg gcatgtcgcc aaaaattcga tcaactttgg ccgggtaaat tttttacggt
     6781 aaacagcctt cccgcgacga tcctggcaat gaatatggaa agagttttta cccagatcga
     6841 taccaatgag cgcaatgttt tccatgatag ttctccgaat gaaagcctgt cctcagcata
     6901 gtaccgggaa ggagggagtg accatctcat taaatatcct acgttaagcc tgttttttaa
     6961 gcaggtatga caggcagcta tgagcgagga gcagacatcc ttgacctgtg aatcatgaat
     7021 attattatct ctcaggctta ttaaacagac ttagggagga ctggtgttgg gctggaaatt
     7081 actaatcggc tttctttttt tcaccatcat tgggtttgca ctatttggtt tcattttttc
     7141 actccagcaa ctgcagtaaa atgatcgtcc cccctgttgc agggccatcc atgaaacaca
     7201 aaaacccgct cagggcgggt tttttatcac gttcacttat ctgctcaggc cagtgccgtc
     7261 atcgctgtcg taagcgtctg tgtcgttatc ggcaattaaa gcccgacaac atttccggca
     7321 gccggacctt tggcaccatt ctcaatcgag aactcaacct tctggccttc gtcgagcgtt
     7381 ttgtaatcgt tgctctgaat ggcagagaaa tgaacaaaaa catctttgct gccgtcagct
     7441 ggcgtaatga agccgaaacc tttgtcagag ttaaaccatt ttactaaacc agtcatttta
     7501 ttcgacatag atacttcctt taattttctt gagccacacg ttgtggcgaa catggcctgc
     7561 ttgcagatga ggacttattg ggcacttagg aggaggctca tgcagaagag tatctaagga
     7621 taacgcttga aatgaggact gctttactaa aactgctttc ataaggtctg tatgacaaac
     7681 cgatagcgca tagtggactc atatgagttg ataagcaaga ttatttttgt cagtaatctg
     7741 ttgggaaaac ttacaattca aagaggtcag taatcttaaa agccctgacc attgcagcca
     7801 gggcttttaa ggtactacag acttcagatt taacattaat ggacttaata aagtgatact
     7861 gctatttata ctgctttcaa ggtaatgact ccaacttatt gatagtgttt tatgttcaga
     7921 taatgcccga tgacttggtc atgcagctcc accgattttg agaacgacag tgacttccgt
     7981 cccagccttg ccagatgttg tctcagattc aggttatgtc gctcaatgcg ctgagtgtaa
     8041 cgcttgctga taacgtgcag ctttcccttc aggcgggatt catacagcgg ccagccatcc
     8101 gtcatccata ccacgacctc aaaggccgac agcaggctca gaagacgctc cagtgtggcc
     8161 agagtgcgtt caccgaagac gtgcgccaca accgtcctcc gtatcctgtc atacgcgtaa
     8221 aacagccagc gctgacgtga tttagcaccg acgtagcccc actgttcgtc catttcagcg
     8281 cagacaatca catcactgcc cggttgtatg cgcgaggtta ccgactgcgg cctgagtttt
     8341 ttaagtgacg taaaaccgtg ttgaggccaa cgcccataat gcgtgcactg gcgcgacatc
     8401 cgacgccatt catggccata tcaatgattt tctggtgcgt accgggctga gaggcggtgt
     8461 aagtgaactg tagttgccat gttttacggc aatgagagca gagatagcgc tgatgtccgg
     8521 cagtgctttt gccgttacgc accacgcctt cagtagcgga gcaggaagga catctgatgg
     8581 aaatggaagc cacgcaagca ccttaaaatc accatcatac actaaatcag taagttggca
     8641 gcattaccgt caatgtatac tgatcgcttc aaattaatcg ctcatgcttt taatgaaatc
     8701 atctaagtac caagccttag agaaatcact aagatcttcc atctgttttc ccattacacc
     8761 cagaagccca ccctcccaaa cactataagc tctctctcca tttaacatat acacattttc
     8821 cggaattgtt cttaatgtat acacggggtt ttgaatacca tattcctgcc caagagtttt
     8881 aaaatacccc ccttttaagt aaaacttatc tttccctctg taactaagat aaaccttact
     8941 aaaatcatca tctttcactt tttcagcaaa ttgcagcatc accctattaa catccagtga
     9001 actattttct gcagaaacag aacgaaggtc ataattaatc tcggaagaga ttacaaacca
     9061 cttataataa acccaaacgc taataccatc attccttgga tcttctttca gaacctcatt
     9121 catgtgcctt tgaagaaaaa tatagttggc agataaaaca ccaccacata ggagtactat
     9181 taatatgatt gctttatata cttttttcat ttctataatc catctcaact gctctaaagt
     9241 ttaccctgag atttttaagc taaggtgcca taagttagca aggaattaag cattgattta
     9301 ccataatgcg gcattacgcg caccgctaaa acggcctcag ggcgtttctg acgggaaacc
     9361 ggtaaaacag gcaacaaacc acccgaaaac ccgccagaaa cgcgcaggct ctgtttttac
     9421 ggcatgcatg agtatgcgcg caagtgcata gcgcggaaat tgacaccatt cagccagggg
     9481 aagtcctgtt tccggcgttc ttcctatccg aaaacgttga acggggacgc aatttattgc
     9541 gtggccgtga agcgttgccg gcgcagccgg aaaaccggtt ttcagcacgt tcaggaatcg
     9601 ggttcgtcga cggccggcaa tcacgttatg gcaagtcgcc ggacgactca ccacacgatt
     9661 caccggcgat tcgactcgtg acttgactct agagtcacca cggtcaaaaa aacgccaaag
     9721 cgacgattcg actctagagt caagtcgtca taagttactg atagtgtgga gtgagtcgtc
     9781 gatgactcta tgacgagtcg tcggcaagtc gtacacgaat caccgacgac ttggattgac
     9841 cttacagaag gtaatactgc gtaatttaat aacaaccaac taactgatat taatgagtta
     9901 tctgcaaaaa tttaattttg cgtggggtgt ggatattttt agtgtgagga gcgttcagag
     9961 atctggcgat ttttgggaag gttcaggaac gccccgggat ggccggagag gaaaactcaa
    10021 gcatggcggg atcaggattt tttccagaat ttccaccacg gggaatgggg tggtgaagaa
    10081 ggttcgtgct tgtgctccag aagctgcata gcctgacgaa gggaatcaat atgctcatct
    10141 cgagccttaa cagccccttt aagaccatcg ttttcagctt caagtaaagc gattctatgt
    10201 tgtaaatcat gcaaaccaac gctgttaata cctgtagatt ctgcgtcact ttttacatca
    10261 tattttacag catcgattac accatcaaga gaaccaaaca ctctaataag ctctgatgta
    10321 tcaataactt taataccctt cccgtgcttt acaacagagg tgtaacttaa cttaccactg
    10381 ttaatcatct tgtaaatagt agg
//
