LOCUS       NZ_CP011338            14400 bp    DNA     circular CON 01-MAY-2024
DEFINITION  Escherichia coli O104:H4 str. C227-11 isolate 368 shch plasmid
            unnamed, complete sequence.
ACCESSION   NZ_CP011338
VERSION     NZ_CP011338.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03856947
            Sequence Read Archive: SRR1980037, SRR1980038
            Assembly: GCF_000986765.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O104:H4 str. C227-11
  ORGANISM  Escherichia coli O104:H4 str. C227-11
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14400)
  AUTHORS   Beaulaurier,J.A., Zhang,X.-S., Zhu,S., Sebra,R., Rosenbluh,C.,
            Deikus,G., Shen,N., Munera,D., Waldor,M.K., Chess,A., Blaser,M.J.,
            Schadt,E. and Fang,G.
  TITLE     Single molecule-level detection and long read-based phasing of
            epigenetic variations in bacterial methylomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14400)
  AUTHORS   Beaulaurier,J.A. and Fang,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2015) Genetics and Genomic Sciences, Icahn School
            of Medicine at Mount Sinai, 1425 Madison Ave, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000986765.1-RS_2024_05_01
            Annotation Date                   :: 05/01/2024 02:09:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,400
            CDSs (total)                      :: 5,274
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 136 of 316
            Pseudo Genes (incomplete)         :: 195 of 316
            Pseudo Genes (internal stop)      :: 60 of 316
            Pseudo Genes (multiple problems)  :: 68 of 316
            Pseudo Genes (short protein)      :: 2 of 316
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP011338.1.
            Source DNA is available from Dr. Matthew K. Waldor at Havard
            Medical School (mwaldor@research.bwh.harvard.edu).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..14400
                     /organism="Escherichia coli O104:H4 str. C227-11"
                     /mol_type="genomic DNA"
                     /strain="C227-11"
                     /isolate="368 shch"
                     /isolation_source="stool sample from a 64-year-old woman
                     from Hamburg who presented with bloody diarrhea and did not
                     develop hemolytic uremic syndrome (HUS)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1048254"
                     /plasmid="unnamed"
                     /geo_loc_name="Denmark"
                     /collection_date="2011-05-18"
                     /note="also called BCG-1 (Russian)"
     gene            141..263
                     /locus_tag="AAF13_RS33065"
     CDS             141..263
                     /locus_tag="AAF13_RS33065"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_275042171.1"
                     /translation="MSGIDLTVEPVGVGIAAGVDGDTLFSPEWVSWFVLAICCR"
                     /name="hypothetical protein"
     gene            complement(1222..1815)
                     /locus_tag="AAF13_RS33185"
     CDS             complement(1222..1815)
                     /locus_tag="AAF13_RS33185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001387475.1"
                     /translation="MKNNNAPLYLWLLWCTLCLYITTPWQYLRYPYTDNLSLPYMLYFC
                     VVVVICIFPLLFRCVLLIVRSASKYDFTFFVKKKKGSKKSTIHLSPYADTRCPKSMRKH
                     WDVLLSLISKAINNDEIIVMHSHLFTPARINKLLKRLESYDLSFSVITYPRKTSLYEKL
                     SIPFIYLLFQWSFPHLHSDGMTVVIRPLQTHHTN"
                     /name="hypothetical protein"
     gene            complement(1939..2193)
                     /locus_tag="AAF13_RS27555"
                     /old_locus_tag="AAF13_27550"
     CDS             complement(1939..2193)
                     /locus_tag="AAF13_RS27555"
                     /old_locus_tag="AAF13_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="WP_001136187.1"
                     /translation="MPQQHPGRLQILVVDAHCKRRLFSTKTPTDPDELARRFCTPDNCL
                     VVVLRDNRFLFRLERAPGSHCRWHKGSSSRHQHLQDWLS"
                     /name="PilI type IV pilus biogenesis protein"
     gene            complement(2365..3049)
                     /locus_tag="AAF13_RS27560"
                     /old_locus_tag="AAF13_27555"
                     /pseudo=""
     CDS             complement(2365..3049)
                     /locus_tag="AAF13_RS27560"
                     /old_locus_tag="AAF13_27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /name="conjugal transfer protein TraC"
     gene            complement(3303..3836)
                     /locus_tag="AAF13_RS27565"
                     /old_locus_tag="AAF13_27560"
     CDS             complement(3303..3836)
                     /locus_tag="AAF13_RS27565"
                     /old_locus_tag="AAF13_27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="WP_001372168.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITRK
                     IRRADSRCCYRERIFAIFPGYFFILANFDIQPVSALRRHSAFIDFVKFGGEIKPVNKDI
                     VDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLELVSN
                     AEHHGFMERLVNIP"
                     /name="transcription termination/antitermination NusG
                     family protein"
     gene            complement(4278..4565)
                     /locus_tag="AAF13_RS27570"
                     /old_locus_tag="AAF13_27565"
     CDS             complement(4278..4565)
                     /locus_tag="AAF13_RS27570"
                     /old_locus_tag="AAF13_27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="WP_000074855.1"
                     /translation="MSPQMIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQN
                     CRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
                     /name="conjugal transfer protein TraA"
     gene            complement(<4603..4761)
                     /locus_tag="AAF13_RS32930"
                     /pseudo=""
     CDS             complement(<4603..4761)
                     /locus_tag="AAF13_RS32930"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /name="ash family protein"
     gene            5719..6750
                     /gene="repA"
                     /locus_tag="AAF13_RS27585"
                     /old_locus_tag="AAF13_27580"
     CDS             5719..6750
                     /gene="repA"
                     /locus_tag="AAF13_RS27585"
                     /old_locus_tag="AAF13_27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="WP_000907869.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERKR
                     TKRRRGEHSTKPKCENPSWYRPERYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSLHP
                     FYVQKRTYAGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVSRLAREISPKDSKG
                     KVIPELEVTVSRLSRLLAEQVRFGVLGVSEETLWDRETRQRLPRYVWITPAGWQMLGVD
                     MVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHRREKAA
                     ASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLELTLAAP
                     PPH"
                     /name="repA"
     gene            complement(7820..8329)
                     /locus_tag="AAF13_RS27590"
                     /old_locus_tag="AAF13_27585"
     CDS             complement(7820..8329)
                     /locus_tag="AAF13_RS27590"
                     /old_locus_tag="AAF13_27585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000045395.1"
                     /translation="MSKSGAEKIQFILNNHEQAYRRLKPVYTFLELASWISVVLLIIYL
                     SGKNHVLTAAFLCFAWTAFWVLFLLHYFPKVFFILDRQSLLNLMEATKDSPDVKQELLN
                     RLFSGKRMTGRDEMDICRLLRKNEEEKIRQSELNIIRNFIDEQQLTNSDIPKKSTVSNE
                     DKKGIR"
                     /name="hypothetical protein"
     gene            complement(8385..8987)
                     /locus_tag="AAF13_RS27595"
                     /old_locus_tag="AAF13_27590"
     CDS             complement(8385..8987)
                     /locus_tag="AAF13_RS27595"
                     /old_locus_tag="AAF13_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_000517687.1"
                     /translation="MGKNQEERKTPIIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAESA
                     PAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEGGG
                     FIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGLDGH
                     PKGQVSDSEAQSAGRRLATLEQKLLRMQAQQENISGQ"
                     /name="ProQ/FINO family protein"
     gene            9340..10686
                     /gene="yagA"
                     /locus_tag="AAF13_RS27600"
                     /old_locus_tag="AAF13_27595"
     CDS             9340..10686
                     /gene="yagA"
                     /locus_tag="AAF13_RS27600"
                     /old_locus_tag="AAF13_27595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YagA"
                     /protein_id="WP_001132021.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMALHNLAN
                     DILREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLSGN
                     EEETSSGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGESSSVRKKRPRLARLL
                     LSLIDDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRSLSEIIHFRPGMSER
                     SQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVRGISIN
                     GEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTLTALLNV
                     ASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVVIETMGYE
                     DSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFEKHMYGVFMHLRY"
                     /name="yagA"
     gene            complement(11174..>13957)
                     /locus_tag="AAF13_RS27605"
                     /old_locus_tag="AAF13_27600"
                     /pseudo=""
     CDS             complement(11174..>13957)
                     /locus_tag="AAF13_RS27605"
                     /old_locus_tag="AAF13_27600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017038493.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /name="Tn3-like element Tn3 family transposase"
     gene            <13971..14315
                     /locus_tag="AAF13_RS27610"
                     /old_locus_tag="AAF13_27605"
                     /pseudo=""
     CDS             <13971..14315
                     /locus_tag="AAF13_RS27610"
                     /old_locus_tag="AAF13_27605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016943399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /name="WbuC family cupin fold metalloprotein"
     Typing          5720..6750
                     /name="IncI-gamma/K1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atatgaccat ccagtgcgtt gatgccatgc tttcgcattt tgtttcttct gcccacttaa
       61 taatgttttc ccttaatgta gtgcctgccg gcgcacgcca ctctttaccc tgagataccg
      121 gtttgacagg tgtcccggtc atgagtggga tagacttgac tgtagagccg gtcggagtcg
      181 ggattgctgc gggcgtagac ggagatacgc tgttttcccc tgaatgggtt tcgtggtttg
      241 ttctggctat ttgctgtcgt tgaagactcc ggggaagtgg atgtggttac catggctgct
      301 ggcgagatag tggttgccgt gtcctgtgcc ggtgcagcag ccgatgttgt gacggtcaac
      361 gttttattat cccagtccag atggccccag aggtgttgtt cttccagcaa ccggttcaga
      421 gaacgggtcc actgatcatt ggcagaccat gaaaccagtt tggtattcag tttgggcgtg
      481 gctgcattct cttgtctgaa tttccatcct tccggtagta atgtttttat ccactgactg
      541 acggtccggt tacgggccgt cggcgatagt gttacagggg ctaccaggct tgcctgtaat
      601 gattaaccgg agaaattctg acttattgtt ggtgaaggta acggtcggat tcgcttactg
      661 gctgtgctgt ttttactgtc tctggcacgc tgactctcgg ggcggagtta ttttaggtgg
      721 agaaagtcgt tgttgcgtaa gtgccaggac gccagcactc tcactgattt gtccgtcaac
      781 aaaaaccaga gcaggcgtat ctgatggtgc cacgtttatt gccggccgga aaattccggc
      841 acagccggac aataatgacg gcagtataag tgtcagatag tttttttttc ataacggaga
      901 ctccgtgttc gtatatacgt gtttgttagt taatgcgggc tttaagtaag ggttcattat
      961 tttatgtgca attggatggg taatgtatac cggtattgga gagaattact cctgaatgtc
     1021 ccgttttagt cgcattcaga agtctgcttg ggtctttaat attgttgatt agtattttca
     1081 ttattctatc ttttgaattt taatgttgca tgttatttta aaggtgaggt tgtggatagc
     1141 caattaagcc agtgttttgg gattttttgt gaaaataaaa atcctgctta tgagcattct
     1201 ttttatatct aaatgtattt tttaattggt gtggtgggtc tggagaggac gaatgacgac
     1261 tgtcattccg tccgaatgca aatgcgggaa agaccactga aacaagagat atataaaagg
     1321 aattgataat ttttcgtaca gacttgtttt ccgtgggtat gtaataactg aaaaactcaa
     1381 atcataagat tccagtctct ttaaaagttt atttattctt gctggagtaa agagatgtga
     1441 atgcatgaca atgatttcat cattattaat tgcttttgat atcagagaaa gcaatacatc
     1501 ccagtgtttc ctcattgact tcgggcaacg tgtgtcagca taggggctaa gatgtatagt
     1561 tgatttcttg cttccctttt tcttcttcac aaagaatgtg aagtcgtatt tagatgcaga
     1621 ccttacaata agaagtacac atctgaaaag tagtgggaaa atgcaaatca caaccaccac
     1681 acagaaatat aacatataag gcaatgataa gttgtcagtg tatggataac gtaagtattg
     1741 ccatggggta gtgatgtaca gacaaagcgt acaccaaagc aaccagagat ataatggtgc
     1801 gttattattt ttcatgttgg ctgaggcagg cgactaaaat aacagactaa ggaggattaa
     1861 ctgtttaatt ctttggaaca aaaaatgcag ttcctgatca tcacacacca ttgaataaac
     1921 attttgcttc ggttgcgttc atgatagcca gtcctgtaga tgctgatggc gggagctgct
     1981 gcccttgtgc cagcggcagt ggctgccggg agcacgctcc agtcggaaca gaaatcggtt
     2041 gtcgcgtagt accaccacga gacagttatc cggcgtgcag aaacgtcggg caagctcatc
     2101 ggggtctgtc ggtgttttcg ttgaaaacag cctgcgtttg cagtgtgcgt cgacgaccag
     2161 tatttgcagt cgtccgggat gttgctgtgg catcaaagac tccttccgga acgggaaaag
     2221 aagcagctgt accgtcataa ctgcacgtac cggttgcgaa aaaacggcac aagcgagtag
     2281 tgacagaaag aatgtcggaa acggcaggaa gggcggtaca ggctgcgaag actggaaacg
     2341 gggtatgccg gggattacac atcctcagtg taatgtgata gtcgtctgct cctgtgttgc
     2401 accccagaca tcttctggca agctgggcag cccgataaat agggtgtgaa agagggtttc
     2461 tctgcgttcg atcccgaatt gatgttcgta tcctctcacc acctgaaatc cttcgtcgga
     2521 gaatattaac tctgccgttt ccagaagctg catagttttg cggtgtagtg gagtgatcag
     2581 ctgcaacgta actggtgccc tcatatcgcc tgtcggtaga aaacactctt cggtgcgact
     2641 tagttgtagc agatggcgtt tcccggaaca ggcccgttca atggattcat tgaacaccat
     2701 ctcaggccat gggcagacat aagtgcgcca gcctgctttc tgtaatttac ccagcgcagc
     2761 ccgaaaacga aacaacggac attccccgac agtggcagag ctgagaaaga gcgtattcag
     2821 cgttccgatg atgggaatat tgcgatcttt ttcggtcaat gctttttaac gccatgattt
     2881 cgccagggag actactgaac tcaggatgtc tgctggcacc agaaagccat tcaagcaggt
     2941 aatgccgtaa tccggtttca ctggttatat tgtggttttc ctgtgccgtt ttcagggcat
     3001 tcagtgcaca ccagagcaac tggcgcacgg gatgttctgt cgtggtcatg cgaatgtcct
     3061 actgtgagat gaaaagaaga ccggtatccg ttctctccgc cacacgtgat ggcacgggcc
     3121 ggcctttgat aaaacgaatc aatactctgg ggtgaagcct tttatttctt tcgccattaa
     3181 gaaatacacg gttccatcaa aagacagggg gagtccatgg tggggatggc ttcgtgcagg
     3241 cttcactccg gaatattctc ttgaattgaa aaaagcccgg cccgaaggcc gggaaatact
     3301 ggctatggga tgtttacaag gcgctccata aagccgtgat gttcggcatt tgataccagt
     3361 tccaggagca gggatatcct cgactcaggc tggagagtat tttccattct gaggagatac
     3421 tgatattgtg cttttgtcag ccatatgctg gaagccgcat tcagctcttc cctggctccc
     3481 ggatttagca cagggtccgg gtaaatcttc attaatccat ctacaatatc tttattgacg
     3541 ggttttattt caccgccaaa cttcacgaaa tcaatgaagg cgctatggcg acgtaatgcc
     3601 gatactggtt gaatgtcaaa attagccaga ataaaaaagt accctggaaa aatagcgaat
     3661 attctttcac ggtaacagca cctggagtct gcccgtctta tctttcttgt tattaatgga
     3721 gtccatggta cgacgttttg ctcatttagc catgagaata aagattctct gtttttgccg
     3781 gctgtattat attgcgcaag ataccagttc cggttattca ggtgttctat attcacgaag
     3841 ttcaacgctc cgcgcaaaac cgatccatga tggacgattt tgcgatgtca ggcgaatatg
     3901 ctagatcata tgtctgaagc agtgtccagc ccatatctgg agaaactgca gttctgacag
     3961 gcatataacc agcagactct agtacgttct gtatcggtga tttggcgaac tcacagataa
     4021 agaacggcta agcactaata tcagcaatac agtggcacag tattgtgata cgcgaaataa
     4081 aacatcagca cgtcagttca gagtaaggtc gccatatatc gggcatgagc cagatacaaa
     4141 taattgggac accgtctgaa gtgatttgct ttattaaagt aacttgtgct tggtgtttat
     4201 gtttatcaat ttgggagcgc ctgtaaagtg caaaatgcaa tttccatgaa agtgtcccct
     4261 ttaatgggga cacctgttca cattttttcg ttttctttag ctgtattgct aatcatgaaa
     4321 acgaacgttc gcatcagcac agcagagcga agtgcatcat atccttccag atttaagttc
     4381 ttcaggcttc gcttcaggta ggttcgtacc gtttcgggag aaagccgaca gttctgagca
     4441 atggcgtcgt aaggaacacc cattgcgtaa aaaacgcaga ctttaagctg gtttgttgat
     4501 agctcgggaa acatgccttc caacagagtg atggccggga gtttctgtat catttgaggt
     4561 gacatagcaa aggtctccga tgacaactaa aaacgctatc accgagttcc tacgctcatt
     4621 ggtgatagcc cagacggggg taggaatacc ggcgtcatcg gaaaccggcc aggctaatgc
     4681 ctgccccgcc tgagctacca tagatgacga caagaaatac aagttcctgt ctttatggta
     4741 tggcaaaaaa cgacgccaaa tgaagaaatt aacttctgcg ccgacgatga ctacgcgggt
     4801 tcctacgccc gatcacagtt ttgcctgtga ccagtaaagg gtacttagta taacgtaccg
     4861 agtcaactaa ccgcttaaaa aacatacaaa aaagcgtcaa aacttaacgc tccgcgccga
     4921 agcgatccag gcatgatgct tctgctttgt ggcgttcgaa catgctggtc catatgcacg
     4981 aaacggtgtc tgaccctcgt ccagacaatc cgcagttctg acgaacatat gaacaaatat
     5041 gctcttgtag aggctgttgt tacacaattg gcgggttgtg taacaaatgg ttttattatc
     5101 taagaatcat tctatttacg atcgaatgat ctacaaaatg attttttaaa ggttttttag
     5161 atcgtttaat tgtcttaaaa cagaatatta ttctgttagg ttgatcggtt gtaagataaa
     5221 tgatcgtgag tagaacaaaa gtcgatcttt taatccaaaa gaaggatcta aagcagaaaa
     5281 atctgctttt aaaaatagat tttatttttt tgacgcaggt caagattaac tcattagagg
     5341 tatcggtgag gaggcactgg aagagaagag atcgttgtaa tgcttttcaa attaacgtaa
     5401 agcgggtata tttcggctgt tattagctgt gcagagggtg gcactctgtg gagcaaagcg
     5461 gcgaaagccg gacggtagaa tgcaccataa ggcattcagg agagatggca tgtacgggca
     5521 gtaagtcaga agactgaaga tgctccggaa gccataaaag gaaaaccccc actatctttc
     5581 ttacgaactt ggcggaacga cgaaagatag tgggggcctc acagaatacg ggtaaagtat
     5641 aatgaaaccg taccagagat tcaaccctgt gcagtgtata aatacacggc acaatcgctc
     5701 cgccataagc gacagcttgt ggcaggtctg aagaatactc catataacgc agtacactgg
     5761 agtcagttag cacccgaaga gcagatccgt ttctgggaag actatgaagc gggaagggcg
     5821 accactttcc tggttgaacc ggaaaggaag cgcacgaagc gtcgtcgcgg tgagcactcc
     5881 accaaaccca aatgcgaaaa tccgtcctgg tatcgtcctg agcgctataa ggcgctgagc
     5941 gggcagctcg ggcacgccta caaccgtctg gtgaaaaagg acccggtgac cggcgagcag
     6001 agcctgcgca tgcacatgtc tctgcatcct ttttacgtgc agaaacgaac gtatgccggt
     6061 cgcaaatatg ctttccgtcc ggaaaaacaa cgcctcctcg atgccatctg gccggttctg
     6121 gtcagcttca gtgatgcggg cacacatacc gtaggcatga gtgtttcccg tctggccaga
     6181 gaaatcagcc cgaaagacag caaggggaag gttattccgg aactggaagt gacggtctcc
     6241 cgcctttccc gtttgctggc cgaacaggta cgttttggtg tgctgggtgt ttcagaggaa
     6301 accctgtggg accgtgaaac ccgccagcgt ctgccacgtt acgtctggat aacaccggca
     6361 ggctggcaga tgctgggcgt cgacatggta aaacttcacg aacagcagca gaaacgactg
     6421 cgtgaaagtg aaatccgcca gcagctcatt cgggaaggtg ttctgcgtga ggatgaagat
     6481 atctccgtac atgcggccag aaaacgctgg tatctgcagc gcagccagga tgcactgaaa
     6541 caccgtcgtg aaaaagcggc agccagtaag cgcgccagac gcctgaagaa actgcctgcc
     6601 gaccagcaga ttcatgagat ggcagagtat ctcaggaagc gtctgccgcc ggatgaagcc
     6661 tatttttgtt ccgatgacca tctgaagcga ctggccatca gggagttgcg tcagcttgaa
     6721 ctgacgctgg ctgcgccacc gccgcactag acagcaccat tccttcagca ctgaatcatc
     6781 gccagcccct ccggggcttt cggcgctggt tccactcagc ccaaaatccg cagtaatcac
     6841 cttaaatccc ctcagagggg catatctgcc cataaaacca cgcatcagtc atcagaacgt
     6901 ggccacgttg tttcagttat ccacataaat ccgcaaacaa agaactttaa gaagccgcaa
     6961 acctaaaaca gcaaacctgc aatatagtct taaccccatt atttaatccc ctgcgttgct
     7021 tcgccgcagg gaaagtcttt atctctgaaa ccactgtgaa taaatacaaa gaggccttcg
     7081 cttgcagcgg ccaaggccgc gccgctcaga atctaaaagc acctcccacg ctgacgcgcg
     7141 ggccccggaa cctcaccgtt ctgagaccac agcaaaaaaa catcaggaat aaaaacacca
     7201 cataaatgca gcaccgtgcc cacccctcat aactgaaaag cgaggccgcc cccgcccaaa
     7261 gggcgggaac aacatcgctt ttaattatga atgttgtaac tacattatca tcgctgccag
     7321 tcttctggct ggaagtcctc catacacgct cgtaagcggc cctcacggcc cgctaacgcg
     7381 gagatacgcc ccgactgcgg gtaaaccctt gtcgggacca ctccgaccgc gcacagaagc
     7441 tctgtcatga ctgaaaacgg gtatggtcta gcagggctgg ggatgggtaa ggtgaaatct
     7501 atcaatccgt accggcttac gccgggcttc ggcggtttta ctccggtatc acatgtaacc
     7561 acagcgtgcc gccttccatg ccgctgacgc ggcatatcct ggtgacgata tctgaatcgt
     7621 tatatactgt gtataatata tggtgcgtta atttttaaac ccttatttaa tttcctcgtc
     7681 agttagatat tgtccgtttc ccaaacgaag cccttcagag acgtcgtgta aacacggtcc
     7741 ttttatctta caggttaacc cctatcaatc ttaggatgaa accacgccat cctctgcttg
     7801 cctggttatt aatttcatat catcttatgc cttttttatc ttcatttgag accgtacttt
     7861 tcttcggaat atcactattc gttaactgct gttcatcaat aaaattcctg atgatattca
     7921 gttcactctg acggattttt tcttcttcat tttttctcag aagacggcag atatccattt
     7981 catcccgacc ggtcattctt tttccggaaa aaaggcggtt aagcagctcc tgctttacat
     8041 ctggtgagtc tttggtggcc tccatgaggt tgagcagact ctgacgatcg agaataaaga
     8101 aaacttttgg gaaataatgt aaaaggaaaa gaacccagaa tgctgtccat gcaaagcaga
     8161 gaaaagctgc tgtaagcacg tgattcttgc ctgataggta tataatcagc agaacaaccg
     8221 aaatccaaga tgccagttct aaaaaagtat atacaggctt caggcgtctg tatgcctgtt
     8281 catgattatt caggatgaat tgaatttttt ctgcacctga tttactcata ataattctct
     8341 tcccattttt ctgatacgta atgtccagct gttatcagga gtgtttattg cccagaaata
     8401 ttctcctgct gtgcctgcat cctgagcaac ttttgttcta atgtcgccag tctgcgaccg
     8461 gcactctgtg cttcagagtc tgagacctgc cctttggggt gaccatccag gccataacgt
     8521 gggacgccgg cgacagctgt ccgctgcaga tatacgcggc gggatgtgat gcaggacact
     8581 gcacagagcc attcgtccat ggtcagttct gtctccgggt gttctgccag aaatgccgaa
     8641 atatcattgt ttatgccaat tttcagggga ataaatccac caccttcagc acgaagatga
     8701 ggaaacaggg cctttatttt ttccacgcac tcgtgagtat actcacgggt ccagtgggag
     8761 ggacgtggaa aacggtgtct tttctttttc ttcttcctgg ctggcgcttc ggggatatgt
     8821 gtttcgacgg cagatgaatt tattccggcg ggggctgatt ctgccgtctg ttcagtaaat
     8881 acaggtgcga taatatctgt tttttcggat aaggacggtg gcgaaaaagt acgacgtttt
     8941 ttcaccacaa tgatgggagt tttcctttct tcctgatttt ttcccataaa tacctcggct
     9001 ggctggtttt actctttaat aatatcacaa ttcataaatt agtaaatttc ccatcaggtg
     9061 atttttcatg gctaataagt ccgttctctg agtatttata ctcgaggtag taacagttat
     9121 ggcgtgacag tcgaattgat attctactga accctgacag aaacatggtt gatatattct
     9181 ctcactttaa tgttgtcatg atataaataa aaacaattca tttgaaagga tatcagaaga
     9241 agatatcggg gcgcacagtt catctctgcc tttgtttcat ggggtgttct ttgtatattt
     9301 acttactgtt taagcatgta cataaggagt gctttcatta tgccaatgat acttgtaaag
     9361 aagaatcccc ggggcaaagt gattcgggag cttagcagtg aggaagagac tgcagtaaag
     9421 acggtctgtg gactgaaaaa gccagcaacc atggcactac ataacctggc caatgatatt
     9481 ctccgggaaa tgcgtgagta tgatgcatgg ttacagtgtg actgtattcc gggagattcg
     9541 ccggccatga attttgctgc tcttaaaaac aatacgggta ctctttatct gtccagtttt
     9601 aatcatgagc atgcacctga atgccccatg tatcgtcagt tatccggaaa tgaagaggaa
     9661 acatcttccg gagcaagccg gcatcccgtt agcacccgta taaattacag aaacttttta
     9721 ccacctgatg acagtaattc tgttatcaga ctgcaggccc gctctgctta ccataatggt
     9781 gagagctcct ccgttcgcaa gaaacgtccg cgccttgccc gtttactcct gtctcttatt
     9841 gacgatgctg ggctgaataa actggattcc ctggctaatc cccgtatccg gactaattat
     9901 cgtgagtgtc ttgatgccat acggcaggtt acactgcagc aggagtatat tcggggaaga
     9961 tccttatctg aaattatcca tttccggcct ggaatgtctg aaaggtctca ggagcggtta
    10021 atggaaacgc tggagaattc agaacgacac tggcctgcca gaagaaaaca tatgttcttt
    10081 cagattttta tggcgcagca catctgtcgt gatgctgtgg aaattcactg ggcgaacggt
    10141 aatattcagg tcatcagacc tgtgcggggg atcagtatta atggtgaagc gcagggcgga
    10201 atacgtcccc cttactgggt tattcttgct ttttgccgga gtgccgatgg cagaatcatc
    10261 tgcagtgaag ggtatgctca tgctctttat caactgacat gtccggtgcc tgtggacagc
    10321 aaactggaac gaaacacgct cactgctctt ctgaatgtgg ccagctggct taaaagaaag
    10381 ccaggtacgc cggaattaag tctggaaagg cccctgtttg atacagaagt ttatgttaat
    10441 ggtgaaaaga aatatgtcct gccggatttc attgtcacag caagggctcc tgacggaaag
    10501 acggccagag tggtcatcga aacgatggga tatgaagaca gtgattactg cgcgagaaaa
    10561 tccaggcagc ataccggcat gaagcagatt ggtgttctgc ataccgatcc accgaaatgg
    10621 ctggataacg atcatccccc ttttgagaaa catatgtacg gtgtttttat gcatctcagg
    10681 tactgagata ttttgtggct cagttctgta acttttcccg taacattgtc tgttgttacg
    10741 ggaaagtccg gtttttgtat tgcaccagag aatactcaga ctgtgatgct gccacagcgt
    10801 cagcaggctt tctgaacggt gtaacattac ttcatgttaa tgataatcac tatcattaaa
    10861 tcttgacatg ccattttctc cttaataaat taatactgta tatgtatcca tatgcgtaag
    10921 cagttaattc atttgttttc ctcagaggat gaaggagata ccgaatgtct gaccctgtac
    10981 ggggttcagt gagttagctg gcctggatgt aaccactcgt tcaactcaat tctcttagcg
    11041 tgggaaaatt tcccttttcc aagcggaccc ctattaatat ttgttttgtt ttaggttcta
    11101 ttcaacatag gtctaaaata cattgaagat aacaagataa ggggtctgac gctcagtgga
    11161 acgaaaactc acgttaagca acgttttctg cctctgacgc ctcttttaat ggtctcagat
    11221 gtcctttggt caccagttct gccagcgtga aggaataatg gccgagcata ttgatatgtc
    11281 cgtggcaaag cggggagagg cgtgcgatat cttcatcatt cagtgtttca ccctgcgccc
    11341 ggagatgatc cagggctgcc tgcatataaa tagtgttcca taacacgacg gcgttagtga
    11401 ccagccccag tgtgcccagt tgatcttcct gaccgtcggt atatcgtttt cttatctcac
    11461 ctttttgacc gtgacagatg gctctggcaa cggcatggcg actttctccc cgattaagct
    11521 gggtcagaat gcgccggcgg taatcttcat catcaatata attaagcaga tacagcgttt
    11581 tgttgatgcg ccccacttca atgattgcct gagtcagtcc ggaaggacgt tcacttttca
    11641 gcaatgaacg gaccagcact gaaacctgta ctttgcccag cttcagggag ccagcggtcc
    11701 ggatcatttc gtcccactga aggactattt ttcggggatc tgattgccct ctggcaatat
    11761 cattcagcac gccatagtcg gcatcatggt ccattcgcca gaaaaccgaa gcaccggcat
    11821 cagccaggcg tggagaaaac tggtatccca gcagccagaa aaggccaaag acaagttcgc
    11881 tggcacctgc tgtatcggtc ataatttcgg ttggattcag cccggtctcc tgttccagaa
    11941 gaccttccag cacaaagata gagtccctca gcgtccccgg tataacgatg ccatgaaagc
    12001 cggaatactg atcggacaca aagttgtacc aggtgatccc tctgttatta ccaaagtatt
    12061 tgcggttcgg tccggcattg attgttctga ctggcgtaac aaagcgcatt ccatctgcag
    12121 atgccacttc tcctccaccc catatctgtg ccagtggcag cgttgcctga aaatcaacca
    12181 gtctggcatt agcgctggtg atagtttcag cccgcagata gttcgctttt gtccagttca
    12241 gccggtgtcg ggtcagtgca ggaacatttg atctgatcag tggttccaga ccgatattgc
    12301 aggcttcagc catcagcacg gcgctgatgc tgacgggcag atcatcaact ctggcactgg
    12361 cttcactagc atggaaaaac tcatcagcaa atccggtatg ggcgttaatt tcgagcagca
    12421 actccgttaa atccaccgga gggagtagat cactgatcat tttgctcagt cgtttcagac
    12481 tgtccggctc atcaagactg gcgaggggag aaattgtcaa ccggggcttc gggccagaaa
    12541 catcgagttc gacagcctca ttttcgcaaa gacgtgcagc aacctgtctg taacgactat
    12601 caagctgatg acccagagat tttattgctt cctgcgggtc tgtcgggtgc cccaaagaac
    12661 gataaacctt aatccggttt gcctgccagt cagcaccctg tagtaatctt gcacgaggat
    12721 ctccccaccg gttactgccg gtaacgtaga catccctccg cctcagacta tcctgcagtt
    12781 tactgagaaa gcagagcgtg tatcccctgc gggtgatatg tttttccttg ttaatcacca
    12841 gccgtttcca tgaccgactg ataatttccg ttggtgcgtc gtcaaaaaac tgccgccgtg
    12901 agctgaactc ccggctgagg tagtcacagg cattcagagt ggtaaccccg gcaggtgcgg
    12961 atgaaaattt aacggtattc agcagatggg gcaggaaacg acgaacgcgc ccgtactgct
    13021 ccaccatttc ttcatgaaaa ttatcgtctg agggccgggc aatttcacgg acaagcgtga
    13081 tgatttcagc cagcttttgc cttgggatgt agctgaacac ctcagcacga atcgattcgt
    13141 ccggtgtttc ttctttcagc aggtacgaac atgcgctggc gagcgccaat gcagatttat
    13201 ccagatcctt cagcgagcgg agccgttttt tctgcccaat ctttctggcg tcacggatga
    13261 taacggccag catggcgtcc agaacgtcca atgcatcatc cagcgccagc gtttcccatg
    13321 caaggacaaa ggcaaccaga accgccatcc ttttctgcgg tgacatcctg gcaatattga
    13381 acaccgaagt cataccagca taacgtgcga gatttttcag gcgcacagcc gggagtgtac
    13441 tcaggttttc agcatgcagg ccaaaatcgt tcagagtttt ccagcgttca attgcttcat
    13501 taaacgccgg accactgatg gtcacagggc cctttttcag tgattccagt aaagacaggc
    13561 ggctgcaatc agttggcccc agcagcatct ccagctgtga acgctgttcg gctgacggta
    13621 tcagtgccag tttgttccac aggcgcaacg tcgccttttc ccttacctct gaaatcaacc
    13681 gggtcagcgt agtggctccg gggagaataa tacgatgttg cataagccac cctgtcgcca
    13741 gatcgaaaag caggccagga cgttcgttgc ttatccagct ccgggtatat aaaagacggg
    13801 taaggcgaaa tgtccagggc caggcaaatt cacgatactg atagtgctga cgtatcagcg
    13861 ctgcatgctc acggcgggta ttttccctct gaccgtattc tgcaagaacg gtgatatcac
    13921 gaatcccgag ctgtctggcg gtaaaatgcc ggacgccgga aggaatatga cctgggacct
    13981 acgtgcgccc gcaccgacac cctcacacct tcgagctact gttgccatta aggggtcgtt
    14041 tcgtggtgct gaattttgac gatcggggta ccgtcaccca tcgggcgata ttgggggaaa
    14101 cctgtacggt gctggagatg gccgcaggaa cctggcatgc cgtgctgtcg ctggataccg
    14161 gtggcataat ttttgaagta aaacacggtg gctatcaacc cgtggctgcc gatgactatg
    14221 cgcactgggc tccagcggaa ggagaaccag gaaccacgga gcttatggcc tggtatgcgc
    14281 aagcgcaggt gggcgacagc acttttgccg tctaaggcga taaacaaaaa cggaatgagt
    14341 ttccccattc cgtttccgct attacaaacc gtcggtgacg attttagccg ccgacgctaa
//
