LOCUS       NZ_CP011337            17818 bp    DNA     circular CON 01-MAY-2024
DEFINITION  Escherichia coli O104:H4 str. C227-11 isolate 368 shch plasmid
            unnamed, complete sequence.
ACCESSION   NZ_CP011337
VERSION     NZ_CP011337.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03856947
            Sequence Read Archive: SRR1980037, SRR1980038
            Assembly: GCF_000986765.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O104:H4 str. C227-11
  ORGANISM  Escherichia coli O104:H4 str. C227-11
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17818)
  AUTHORS   Beaulaurier,J.A., Zhang,X.-S., Zhu,S., Sebra,R., Rosenbluh,C.,
            Deikus,G., Shen,N., Munera,D., Waldor,M.K., Chess,A., Blaser,M.J.,
            Schadt,E. and Fang,G.
  TITLE     Single molecule-level detection and long read-based phasing of
            epigenetic variations in bacterial methylomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17818)
  AUTHORS   Beaulaurier,J.A. and Fang,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2015) Genetics and Genomic Sciences, Icahn School
            of Medicine at Mount Sinai, 1425 Madison Ave, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000986765.1-RS_2024_05_01
            Annotation Date                   :: 05/01/2024 02:09:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,400
            CDSs (total)                      :: 5,274
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 136 of 316
            Pseudo Genes (incomplete)         :: 195 of 316
            Pseudo Genes (internal stop)      :: 60 of 316
            Pseudo Genes (multiple problems)  :: 68 of 316
            Pseudo Genes (short protein)      :: 2 of 316
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP011337.1.
            Source DNA is available from Dr. Matthew K. Waldor at Havard
            Medical School (mwaldor@research.bwh.harvard.edu).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..17818
                     /organism="Escherichia coli O104:H4 str. C227-11"
                     /mol_type="genomic DNA"
                     /strain="C227-11"
                     /isolate="368 shch"
                     /isolation_source="stool sample from a 64-year-old woman
                     from Hamburg who presented with bloody diarrhea and did not
                     develop hemolytic uremic syndrome (HUS)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1048254"
                     /plasmid="unnamed"
                     /geo_loc_name="Denmark"
                     /collection_date="2011-05-18"
                     /note="also called BCG-1 (Russian)"
     gene            67..1220
                     /locus_tag="AAF13_RS27460"
                     /old_locus_tag="AAF13_27455"
                     /pseudo=""
     CDS             67..1220
                     /locus_tag="AAF13_RS27460"
                     /old_locus_tag="AAF13_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /name="site-specific integrase"
     gene            1371..2195
                     /gene="traE"
                     /locus_tag="AAF13_RS27465"
                     /old_locus_tag="AAF13_27460"
     CDS             1371..2195
                     /gene="traE"
                     /locus_tag="AAF13_RS27465"
                     /old_locus_tag="AAF13_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="WP_001238932.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGVR
                     EVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPLLA
                     TLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEWPDT
                     ADNFRMLINDSSNTEVIFWHSFPFAGNTKREYCGVILRQEKEINGSVIYPALSLSPTLL
                     NDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAENLITGQILPVSLFNTSLLPW"
                     /name="traE"
     gene            2281..3483
                     /locus_tag="AAF13_RS27470"
                     /old_locus_tag="AAF13_27465"
     CDS             2281..3483
                     /locus_tag="AAF13_RS27470"
                     /old_locus_tag="AAF13_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="WP_000976357.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASSH
                     YGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPNDVSNKADDVKDDWDLFDSAVDNQ
                     HGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGKVND
                     ADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGVTPKY
                     QRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQNIIPRS
                     IDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDVDLTPASGFTSDSKRQFASVGAEF
                     NAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGAMAQLQFT
                     F"
                     /name="conjugal transfer protein TraF"
     gene            3543..4127
                     /locus_tag="AAF13_RS27475"
                     /old_locus_tag="AAF13_27470"
     CDS             3543..4127
                     /locus_tag="AAF13_RS27475"
                     /old_locus_tag="AAF13_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="WP_000977522.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEINA
                     DNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNAVR
                     TIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLARDRL
                     GKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
                     /name="histidine phosphatase family protein"
     gene            4522..4980
                     /gene="traH"
                     /locus_tag="AAF13_RS27480"
                     /old_locus_tag="AAF13_27475"
     CDS             4522..4980
                     /gene="traH"
                     /locus_tag="AAF13_RS27480"
                     /old_locus_tag="AAF13_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="WP_001079808.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQY
                     TLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVRLP
                     LPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
                     /name="traH"
     gene            4977..5795
                     /gene="traI"
                     /locus_tag="AAF13_RS27485"
                     /old_locus_tag="AAF13_27480"
     CDS             4977..5795
                     /gene="traI"
                     /locus_tag="AAF13_RS27485"
                     /old_locus_tag="AAF13_27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="WP_000646098.1"
                     /translation="MKALTSLLLPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSEK
                     PDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEGWL
                     PPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGTEGS
                     VVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQGMITR
                     PDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLIGTEGGSR"
                     /name="traI"
     gene            5792..6940
                     /gene="traJ"
                     /locus_tag="AAF13_RS27490"
                     /old_locus_tag="AAF13_27485"
     CDS             5792..6940
                     /gene="traJ"
                     /locus_tag="AAF13_RS27490"
                     /old_locus_tag="AAF13_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="WP_001024972.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVSV
                     SRFGRRVRCSSATLPTTLMSSLIDELFGREVIPRVLAGNPVDRTIQINGDASGRYGLKR
                     GERIRLRSHLIQGTSGAEEKAISITMRVIPTEIPDILSMNIEPDLLEAMVCKSGLGFVC
                     GETGSGKSTLCSALYRYIMDNFPDAKIVTYEDPVEYILGNENDLLPPHQAEIGRDVVSF
                     AAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLARLLGLF
                     PPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMPHAEWGH
                     HIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
                     /name="traJ"
     gene            6937..7227
                     /locus_tag="AAF13_RS30090"
     CDS             6937..7227
                     /locus_tag="AAF13_RS30090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001314267.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSETTFWICTGI
                     ILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
                     /name="hypothetical protein"
     gene            7242..7793
                     /locus_tag="AAF13_RS27495"
                     /old_locus_tag="AAF13_27490"
     CDS             7242..7793
                     /locus_tag="AAF13_RS27495"
                     /old_locus_tag="AAF13_27490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="WP_000014586.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGSA
                     SALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASREAM
                     KYINLLDIPLRTVDVFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNMPDV
                     AAQYLEHWQDRWNKGTDWRP"
                     /name="phospholipase D family protein"
     gene            7883..11648
                     /locus_tag="AAF13_RS27665"
                     /pseudo=""
     CDS             7883..11648
                     /locus_tag="AAF13_RS27665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /name="LPD7 domain-containing protein"
     gene            11666..12013
                     /locus_tag="AAF13_RS27510"
                     /old_locus_tag="AAF13_27505"
     CDS             11666..12013
                     /locus_tag="AAF13_RS27510"
                     /old_locus_tag="AAF13_27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="WP_001055900.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARAA
                     ADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEKLP
                     GNIDPWKDYNL"
                     /name="conjugal transfer protein"
     gene            12010..12702
                     /locus_tag="AAF13_RS27515"
                     /old_locus_tag="AAF13_27510"
     CDS             12010..12702
                     /locus_tag="AAF13_RS27515"
                     /old_locus_tag="AAF13_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="WP_000138552.1"
                     /translation="MTENTEPSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKALL
                     CTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTSQPAWSQNDAMAFGSQAL
                     ATAFNLDFVHYRSQISSLSPRFSDEGFVGYVNALQASNILETIKKEKMNLTATTGAGVL
                     VRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEIRQMI
                     SRNAGPNS"
                     /name="DotI/IcmL family type IV secretion protein"
     gene            12713..13696
                     /gene="traN"
                     /locus_tag="AAF13_RS27520"
                     /old_locus_tag="AAF13_27515"
     CDS             12713..13696
                     /gene="traN"
                     /locus_tag="AAF13_RS27520"
                     /old_locus_tag="AAF13_27515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="WP_001191879.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNAS
                     QNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAPIT
                     SVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLFDVQ
                     YNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLRIPRQ
                     GPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGDDLLVR
                     SRAMLRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
                     /name="traN"
     gene            13699..14988
                     /gene="traO"
                     /locus_tag="AAF13_RS27525"
                     /old_locus_tag="AAF13_27520"
     CDS             13699..14988
                     /gene="traO"
                     /locus_tag="AAF13_RS27525"
                     /old_locus_tag="AAF13_27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="WP_001271994.1"
                     /translation="MSAEQDAGKSGKKLAALLGLGSIILFGGGYIAFSKLSGSNSDMQS
                     AVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTPEK
                     QQQPAAKPENYAHRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATTMWN
                     KSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSSKVIA
                     TIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSVASNVN
                     TRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGVIAGGMA
                     ERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPSVNRTSQA
                     EQRSQARMQTAMEQRKAVMQRRYDEQPETP"
                     /name="traO"
     gene            14988..15692
                     /gene="traP"
                     /locus_tag="AAF13_RS27530"
                     /old_locus_tag="AAF13_27525"
     CDS             14988..15692
                     /gene="traP"
                     /locus_tag="AAF13_RS27530"
                     /old_locus_tag="AAF13_27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="WP_000801920.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLLA
                     AIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLARDV
                     KTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSRLNGIMTQKSV
                     TESSSTPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQRIDPT
                     TRTIITSAGTLR"
                     /name="traP"
     gene            15692..16219
                     /gene="traQ"
                     /locus_tag="AAF13_RS27535"
                     /old_locus_tag="AAF13_27530"
     CDS             15692..16219
                     /gene="traQ"
                     /locus_tag="AAF13_RS27535"
                     /old_locus_tag="AAF13_27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="WP_001055569.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARARS
                     AGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAAGA
                     ANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFIDRV
                     LNALLASIGIVF"
                     /name="traQ"
     gene            16270..16674
                     /gene="traR"
                     /locus_tag="AAF13_RS27540"
                     /old_locus_tag="AAF13_27535"
     CDS             16270..16674
                     /gene="traR"
                     /locus_tag="AAF13_RS27540"
                     /old_locus_tag="AAF13_27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="WP_000086965.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKYLLLIPMMLLPKAV
                     LADGDLADMVRNVGEGAKTAQSSSITIAQFIGVILFLGGLIGLKKVGKQGGMGLAGCIV
                     SIVIGAVLVAGPEMMSRSQKQLGISSISIG"
                     /name="traR"
     gene            16738..16926
                     /gene="traS"
                     /locus_tag="AAF13_RS27545"
                     /old_locus_tag="AAF13_27540"
     CDS             16738..16926
                     /gene="traS"
                     /locus_tag="AAF13_RS27545"
                     /old_locus_tag="AAF13_27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="WP_001277253.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANIIL
                     EAVKCYTSTEPAHATQH"
                     /name="traS"
     gene            16910..17710
                     /gene="traT"
                     /locus_tag="AAF13_RS27550"
                     /old_locus_tag="AAF13_27545"
     CDS             16910..17710
                     /gene="traT"
                     /locus_tag="AAF13_RS27550"
                     /old_locus_tag="AAF13_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="WP_001164783.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEALLPEPELTTPGRCICCRQNVR
                     HRFRLDDSWPLRQLTDTISNTRVRLNKATEHLVKLIRRGEPVATGKKEKYNTAVKAAER
                     ALEHARLSARRLSLRHVQKAEITSTEPLSEKEQELFHEDGPPYSLCAFCHAWHSLNGYA
                     AAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVEDKFRS
                     FRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
                     /name="traT"
     Typing          4978..5792
                     /name="MPF_I"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.632"
                     /coverage="100.0"
                     /evalue="9.99e-178"
     Typing          11667..12010
                     /name="MPF_I"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="91.379"
                     /coverage="100.0"
                     /evalue="1.6599999999999991e-54"
     Typing          12011..12657
                     /name="MPF_I"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.074"
                     /coverage="91.0"
                     /evalue="5.6e-143"
     Typing          12714..13693
                     /name="MPF_I"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          14989..15689
                     /name="MPF_I"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.573"
                     /coverage="100.0"
                     /evalue="4.47e-153"
     Typing          15693..16216
                     /name="MPF_I"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="3.39e-100"
     Typing          16271..16647
                     /name="MPF_I"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="96.032"
                     /coverage="94.0"
                     /evalue="1.29e-44"
ORIGIN
        1 gtaccggatt ggcacgcttg cagagaatga ttgttggcat tattttttgc ctcttttgag
       61 gttagcatgc cgtctccacg catccgtaaa atgtccctgt cacgcgcact ggataagtac
      121 ctgaaaacag tttctgttca caagaaaggg catcaacagg agttttaccg gagcaatgtt
      181 atcaagcgat atcccattgc cttcggaata tggacgaaat aacaaccgtt gatattgcta
      241 catacagaga cgttcgttta gcagaaatca acccccgaac gggtaaaccc attacaggta
      301 atactgtacg tcttgaactc gcccttctgt catctctgtt caatattgct cgtgttgaat
      361 ggggaacctg tcgtactaac ccggttgaac tggttcgcaa gccgaaagta tcctcgggac
      421 gagatcgccg gctaacgtct tcagaagaac gtcgcctttc tcgctatttc cgcgaaaaaa
      481 atctgatgtt gtatgtcatt ttccatcttg cccttgaaac agccatgcgg cagggcgaaa
      541 tactggcctt acgttgggaa cacattgatt tgcgccacgg tgtggctcat ttacctgaaa
      601 ccaaaaacgg tcactcacgg gatgttcctc tgtccagacg tgcccgtaac tttcttcaga
      661 tgatgcccgt taatctccac ggcaatgttt ttgattacac cgcatccggc tttaaaaatg
      721 cctggagaat agccacacaa cgacttcgca tcgaggacct gcattttcac gatctacggc
      781 atgaagcaat aagccgcttc ttcgaactgg gtagcctgaa tgtaatggag attgctgcaa
      841 tatcaggaca tcgttccatg aatatgctga aacggtatac tcatcttcgt gcatggctac
      901 tggtcagtaa gcttgatgcc cgccggcggc agacacaaaa agtggcagca tggtttgtgc
      961 cgtatcctgc ccatatcacg accatcgatg aagaaaatgg gcagaaagcg catcgtattg
     1021 agatcggtga ttttgataac cttcacgtca ctgccacaac gaaagaggaa gcagttcacc
     1081 gcgccagtga ggttttgttg cgtacactgg ccattgcagc acagaaaggc gaacgtgtcc
     1141 catctcccgg agcgttacct gttaacgacc ctgactacat tatgatttgc cctctgaacc
     1201 cgggcagcac cccgctgtaa acatcattca agacacatac agaaaggcga gttgcaacaa
     1261 ctcgccaaac acattcgatc tgcatgatcg aaattattcg tttcaccaga aaaaacaccg
     1321 aagatacaat aaccacatga gctgaaaaga ctttctcagt cggaggaaca atgagactga
     1381 atacgaccgg tattgcggcc cgaatgatgc tctcgctgga caaaaaagca atcggcgaaa
     1441 aactcatcaa cggtcgtcag ataaacccga cacctctgca ggtccgttac ccacaagggg
     1501 taagagaagt tctggggata atgagcgaac aattagccat atccaccgct gatttaaccc
     1561 gaatcctgct tgaggatgct cttcataata tgtttatgcc tgcggataac accacaggaa
     1621 acataataag cagaattgaa tacatcatgc tgtcgcatga tataaatgcg ccattactgg
     1681 caacattgct gtcgccatgg aatatccgct ccacggttat acaggacccg gccagacttg
     1741 cagactatct ttcagctgac gctctcgaac atcttgctga atgttttaat ctcaatccgg
     1801 actggctgaa tggccatgaa aactatccga tagcgttgtc cggtgaatgg ccagatactg
     1861 ctgataattt cagaatgctt attaatgata gcagcaacac tgaagttatt ttctggcata
     1921 gtttcccctt cgcaggaaac acgaaaagag aatattgcgg ggtaatattg agacaggaaa
     1981 aagaaataaa tggctctgta atatatccgg cactatcatt atcaccaaca cttctgaacg
     2041 atgaaaaaag aaaatggtta actgaatata caaccaggca gaatacaaca atgtctttgc
     2101 gacgcgtcac gttgagacct ggccttgccg agaaccttat tacagggcaa atactacctg
     2161 ttagcttatt taatacgtct ttattaccct ggtaaatacc agatagtacc aaagtaaaaa
     2221 attacggcac atccgtgtca tagcgttgtt ttgtctaaat tctgataaac aggaaattaa
     2281 atgatgaaga aaaatcatat tacccgcacc atcattgcat ctgctgtatt attttccttt
     2341 aacgctgcag cagcgacgtc atattttgaa gcgcgtaacg atgcaatggg tggcaccggg
     2401 gtagcatctt cccattatgg tgtggcacct ctggcaaacc cggctttact gacaaagcac
     2461 aacagcaatg atgatttcag ccttcttctg ccgtctgttg gggcacaagt tgctgatcct
     2521 aatgatgtat caaataaagc tgatgatgtt aaagatgact gggatttgtt tgacagtgct
     2581 gtagataacc agcatggcgt acaacaggca gcggcaaatc ttaaacatcg tctgcaggaa
     2641 ttccgcaata tcaatgcaga tgctcaggta ggtgtatcag ctgtagcagc catggcaaat
     2701 gacactctgc cgtttgccct tatggtcaag tcttacggaa ctgtcagcgt aaacggaaaa
     2761 gtaaacgatg cagacctgga ttatctggac aaagttgcca atggaacaat taccgacgtc
     2821 gataagaatg ccctgacctc acgcgcattc gggcgtgcag ctgtaattac cgacgtcgga
     2881 atttcatttg ccaaagaact ggaaacggcc ggacaaaaat ggtctcttgg cgtcacacca
     2941 aaatatcagc gtgtggacct gtttaactac aacgtaacgg ttcgtgatta cgacaaagac
     3001 gattttgacg gtgacaagta ccacaacacg aaaaacggat tcaacgccga tattggtgct
     3061 tatacagacc tgaatgacaa ctggactgtc ggtcttgttg cccagaacat cattccacgc
     3121 agtattgata ctaaagtcgt caatggcttt aaagaaacct tcaaagttcg tccacaggca
     3181 accgccggtg tatcctggca taatgacctg tttaccactg ctcttgacgt tgatctgact
     3241 cctgccagtg gcttcacttc tgacagcaaa cgtcagtttg caagtgttgg tgctgaattt
     3301 aacgcatgga aatgggcgca attgcgtgcc ggttatcgtc agaacatggc atcaaactcc
     3361 ggaagcgcct tcaccgctgg cgttggtatc tctccatttg acgtcgtaca tatcgatgtt
     3421 tccggcctgg taggaactga ccacgattac ggtgcaatgg cacaacttca gttcactttc
     3481 tgattactgt ccccgccccc ggcactaaac ccgggggtat ttttatattc agtttaaata
     3541 acatgatgaa accacgctct tcttattcaa aaacagcatt cattctttta ttttctgttt
     3601 tcctggtagc ggcagtgaca aaagcaaaaa gctcgcttcc cgatatcacc ctagaacagg
     3661 caaaagaaat taatgctgat aacaccgtta ttttcctttt ccgccatgga gaacgttgcg
     3721 accgttcaga tatgccatgc tattcggata aaagcggaat cactattacc ggtacagaga
     3781 aagcacagca ggaaggtatt aagttcgcaa caatcttcag tgaatatgat atttactcca
     3841 gcaatgcggt aagaaccata caaactgcaa aattcttctc cggtaaagaa cccgtcgtta
     3901 tggacagttt atcagactgc aataatgatc tttataagac gcttgaatca atcgcaagag
     3961 aatctcataa aaggaacata gtaataatga cacataatca ttgtttatca tttctggcca
     4021 gagataggct cggcaagaaa ttcaaaccgg cgtaccttga tgccctgatc atgcattacg
     4081 atggtacccg gctcatactt gacggaaaat acaacaaaga ggcatagagg atatttgaat
     4141 ttacataaat tctgatatac tcagtaagat acaagagtgt acgggcacat gtgcctgtct
     4201 gaattgcagg ttatccggac gagggccggg aactgtttca gggacaagcg ccggtacgtt
     4261 cgattcataa atcgtcgagt catccatcat ggatcgattc ggcgcggagc gttaaagatt
     4321 acgctcatat tcgttttatg tctgacgaca catcaaccag ccagtatcag gaataatcac
     4381 cttcagtaaa ctatgaggct atttacctgg ccacactggc atttatttcc ggtaacgcaa
     4441 gtatagccgg aaacaaacat catcctgtct gtaccggagc accggtatat cccattctga
     4501 tttaactgct caggaattat tatgaaccgt attatacctg cggggtgtct tgccgccctt
     4561 tttctggctg gctgcaactc cccttctcag catactcctg ttccgcgtaa ctacggtgct
     4621 gcctctgatg tggcggttac ccggcacact cagtacacat tatgggacaa aggtgtgttt
     4681 tccggccaac ttccggtttc aacggggtct catcttctga cagctaacag cgatcgcctt
     4741 tcctttgact gggaaggtga tgcaattgaa ttactgaacg aactggccag agtcaggggg
     4801 atgcagttta actacaatgg tgtccgcctg ccgttaccag tcaaccttca cgtccgggat
     4861 atgacgtttt ccaatacgtt gagacttatt gaagcccaga cagcctggag agcaacaatc
     4921 catcaatatc cgggcttgtt acaggtcagc ttcatgcagc cggagaaccg gaaaaaatga
     4981 aagcactcac ctctctcctt ttgccagtaa tactgcttac tggttgttct tcttccccta
     5041 ccatctcgcc aggtggtgtc aatacgccac cacctgacat taatgcatgg cttaaaccac
     5101 gaagtgaaaa gccggatagt ctgtcagaaa cacgctggaa aatgctgacc gacgcaggta
     5161 aaacgcttgg atttcgtggt ggcaaggctc agcgctcatg ggagctgatt caggcactca
     5221 acgcccggga aagcacactt aatgccttat acgatttccg gccactgatt agcccggaag
     5281 gctggctacc accggttatt gatgaagcac aggacgtagc tcatatcacg ccagatcaaa
     5341 tccggacctc ttcccgggtc tggaccatca ttcgccctga acgctttgtc agcaatccac
     5401 cgggctggcg tgactggctt ctccgggggc tttcaacaac agccactccg ggtacagaag
     5461 gcagcgtcgt tccagaagac agcgtgcaac gaaaagtgtg ggaaaccgca ttacggcagg
     5521 gatggcagga aggaagacaa aacgccgacc tgacgcttga agccaaccag aaaaccctca
     5581 cccgtgatta ccggggcatg atgctgtact cacttctctg gcgtcagggc atgatcaccc
     5641 gtccggatgt ttctgatcaa atgcaaaccg ttacaggtga cgggaaaaaa ctggtcactg
     5701 gagatcgtgt tcgtcgcctt aaaaatcatg cggaattcaa tcttcagaaa tctcactggc
     5761 gtccgcttat aggcactgaa ggaggctccc gatgaatatt cctgatgagt ttggtctttt
     5821 tccgtttcag cgtttcactg cagatgaact acggcatttc tttgtctggt gtgccgcaca
     5881 taaagtcagt gatgtggatc tgaccggtgg gtcacctgta tctgtcagtc gctttggccg
     5941 acgggtgcgt tgttccagtg caacgttacc aaccacactc atgtcctccc tgattgatga
     6001 actgttcggt cgggaagtca ttccccgcgt tctggcaggc aatccggttg acaggacgat
     6061 acagattaac ggggatgcat ccggtcgcta tggtcttaaa cgcggagaac gcatccggtt
     6121 acgctctcac ctcattcagg gcacatctgg tgcggaggag aaagcaatat caataaccat
     6181 gcgtgtcatc ccgacagaaa taccggacat cctctccatg aacattgaac cagacctgct
     6241 tgaagcaatg gtctgcaaaa gcggtctggg atttgtatgt ggtgaaaccg ggtcaggaaa
     6301 atcaacgctg tgttcagcac tgtaccgtta cattatggac aatttccccg acgccaaaat
     6361 cgtgacatat gaagacccgg tggaatatat cctgggaaat gaaaacgatc tgctcccacc
     6421 gcatcaggca gaaatcgggc gtgatgtggt cagttttgct gccggactgc gttctgctgt
     6481 acgacgtaat cccgaaatta tcggtgttgg tgagatcagg gataacgaaa ccgccgacgc
     6541 agcggtacag gcaggcaaca ccggacatta ctgcctgagc accatgcata cgaaatctcc
     6601 tggtgaaacg ctggcacgtc ttcttgggct gtttccccct gtcatcaggg attcgatggc
     6661 ctgggctgtc ctgtcactgt tgcagttcat tctggttcag gttctggtca gaacaaatga
     6721 cggtggccgt aaagccgtcc gggaatatat cgtcattaac gatgagcttc gcgacaatct
     6781 ttccggaatg ccacatgcag agtgggggca tcacattgat gccataatcc ggcaggaaaa
     6841 acgtcgtatc cgggatcaga ttctggaaat gtatatccgg aatgaagttg accgaagaga
     6901 agcgattctt tttatcccgc caggggagtt acggtcatga aaagagaagg tttctggcga
     6961 tatgcaacac ctccagtcaa catccagggc gtacctctgc cgttcaccat gatttatctg
     7021 tttatatgcc cttttccctc cgaaacaacc ttctggattt gcaccggcat cattttattt
     7081 ttcgtcattc tcgaccgttc aggctggact gtcaggacta tctgtacacg tattttcagc
     7141 attctgagag gggccatcgc ctccggtcgc ccatggtggt atcgccacca tacggaatca
     7201 ccacgagact ggactggcct cgattaaatt ctggagtctt tatgtcaggt aaaaaaacta
     7261 tcgttactct gctccgggta tcactactgg catgcccgtt actgttcacg acaccatcgt
     7321 ttgcaatgat cgacacgcca tccgtgaagg tcggcttttc tcctgaagga agtgcatccg
     7381 ctctggtgct tgacacaatc aacagtgcgg aaagctccat caggatgatg gcatattctt
     7441 tcaccgaccc ggatgtaatg catgcgctgg caaaagcgaa aaaacgcgga gttgacgtcc
     7501 gtattgttgt tgatgacaag gggaatacga accgggcaag tcgggaagcg atgaaatata
     7561 tcaacctgct ggacattcct ctgcggactg tagatgtctt ccccatccat cacgacaaag
     7621 tcatcattgt tgacggaaac acggttgaaa ccggctccta taacttctct cgtgctgctg
     7681 cacgcaagaa ttcagagaat gtcgttgtgc ttaagaatat gccggatgta gccgcacaat
     7741 atcttgaaca ctggcaggat cgctggaata agggtacaga ctggagaccc tgacaaacat
     7801 ttctcccccg gttttctatc cccccagcca cccccggttt ccggcggtgg tttttttccg
     7861 tcttttttca ggagaagcac tcatgccgtc tgtatcccga taccgcacct ggcttgccgt
     7921 gcctgcagat gaaatcgaag atctcaaaaa agcacatccc ccgatgaacg ggcacacgcc
     7981 cgttctctgg gacaaggacc acaaactctg gtttgcccgc ccgggggcgg acctgtccat
     8041 gctggaccgc tggctgcccc ggccgcagga agtctccatg aacggcagtg acccggtcac
     8101 cgagtttgcc caggtactgg aaaatgccgg gctggttctg aaagaactgc cggtcatgga
     8161 cgggaaaatc caccgtgtcc cgaccgccgg tgacaaaaaa ggacaaaaaa gtggcgcata
     8221 tcgcggcttc ctggacggca gacctgccgg ctggtacagg gattaccgca gcgcggatga
     8281 ttcgccggtc acctggactt tctccggcgg agagcagaca gatccccgcg caagacttca
     8341 cctgaaagcg cattcgatgc aacgcaggga agacgcggag cgggaactga aagcacagta
     8401 caaccgacag gctgcctatg cccgccgcta tgtcagcaaa tggccgcagg ccacggcaca
     8461 tgaatacctg acccggaaag gcattcaggc tgcgccgggg gtgcgaataa acgacaaaaa
     8521 tgagcttgtt attcctttca gcaaccgtaa cggcgcaata cgctcctacc agcgcattcc
     8581 ggtcaccggc gggaaggatg cccgcatcct gaaagattcc gagaaaaccg gtaactggtt
     8641 tgcgctgggc acgccgcgca acggccagcc ggtactgttt gccgagggct acgccactgc
     8701 cgcctccctg cacgaagcca caggccttcc ggtactgatg acagtggatg cgggcaacat
     8761 gattattgtg gccgaagagg cccggcaaaa atggaagcaa agccccttta tcttctgtgc
     8821 cgacaacgat catgcacgcc aggttaacaa aggtatcgtc agtgcaacaa aagccgcaga
     8881 actgacagga ggatcggtca tctttcctgc gttcaccgat gccgaaaaag cgcaggggct
     8941 gaccgatttc aatgacctgg atgccagccg gggacgggcc gcttttctgc atgtcatcaa
     9001 cgcccagtta gaacatatcg gggtttccac ccccaacagt aacaccccgg aaatccggga
     9061 ggcactggtg atcggcaacc tcgtttttac accggtccac actgaggaaa aaaccatgac
     9121 tccgacagaa tatcctgaaa catcacccga tacagggcac agccatgacc agggaccttc
     9181 tctgccgtcc gccacgcagc aggtacaacc tgctgcttcc agcggtaaca ttgctgatga
     9241 aacacagcca tttaccagtc acgccactga aaataacggc aaagatgagc ggcaccctga
     9301 taacgttcag gcgcaaacgg aaagctctgt agttcaggac gcccctgcag aatcaccggc
     9361 atccccggaa agtacaacgg cgtctgcccc tgatgaactc gctcatccgg cggaacctcc
     9421 ggaaaaagtt gtctctgtgg ccacagataa agactggcgt gaatttgaag ccgaactgtc
     9481 acaaccggaa aaaaaacgag ttacaacagg agtccggcac ttcaccagaa ataccggccc
     9541 ctgctgcccc cgccgcccct gaagacagcc cctcatctga aaccatgaat gaatcaaccg
     9601 aaccatcacc ttcccctgat gcagtcaaag aggaaatgac cgtgatgacg gagcctgatg
     9661 acaaacaaag caccggaata tctccggata ctccccggga agaggcggtc agccatgtaa
     9721 ctgaagacat tccccttcag ccagaaccgt tccttcctga gtcagatggc cctgaagaat
     9781 acgaagacta cagcgcatat caggagctga tgaacaatga aaatcctgag catctacagg
     9841 aaaacagtga tatgccgtcg caaccggaca caggccacgt tcaggcagat gagtcagaac
     9901 ttcaggccac aacagccact atagaaccgg ctgtccggga tgaacccact ccgcagcaac
     9961 ctgttcagag cacatcacct tctgataata cttcatcgtt tctcgacaaa gcgcgtggcc
    10021 tttttacgcg taaaaaaacg gattcacagg cgcatgagaa cagtgatccc acgccctcag
    10081 cggaaaccac gacgactcca ccgatacctg acagtattgt ctacgccccg gaacgttctg
    10141 atgcaccgat atcgctcaac atggacgaaa ttcttaaaga gcttgactgg gaaaaacgag
    10201 cagacagaac cgtcctgtac aaactggatg gcaaacctgc cttcattgac cgggtaaacc
    10261 ggctggaaat ggtcaacggt gccagcaatg acgatcgcag cgttctggcc gcacttgctg
    10321 tggccaccaa attttatggg ggcgtcatag agctgacggg cagtgatgcc tttaagcaaa
    10381 aagcaatgca gttgattatc gagcacaaca tcgatgtccg gatgaaattg ccagatcaac
    10441 gggctgaact agaaaaatta cgcaaggaaa tggctgtcag taaggatgct gttgtcaccc
    10501 atcagccaac accggaactt aaccggaata cccctgaaca accagctgtc cctgaccccg
    10561 ttcaggaaaa agaagccaca caatccccgg cgtcccctgc tgccactgaa tcatcaaccg
    10621 tcagcaccgc tcctgcatca tcaccgactg aaccaggcaa aacggcagat gcagcaccag
    10681 gtgaagaacc accaggaaaa cttcgcccgg gagagagcgt gactgccgta ctgcacaatt
    10741 ttggtcgtgc tgaatatgca cccggcaaag gagaaagttt ctttgttgag cttaaaaacc
    10801 gcagtggcag caaactctac tggggggaac aactggaaag tctggtcaaa aaccatcaga
    10861 aaggtgacgt ggtgacactg acacttcaga accgcgaaca attcatcctc ccgggagagc
    10921 aaaaagcacg tttccggaat aagtggtcca tggagtcagt taccaacggg atctcggtat
    10981 cacatgataa tccggataaa ggtcagcgta ttcaggcgat tccggtcgaa acctttatga
    11041 aagtggctgc gcaaatcagc caggggtggc ccgaagagat gaaagcgtta cgtatgccag
    11101 aaaacgtcgg ctcacatctg tttatcggtg aagaccgaca tccggtctcc gcgccacaga
    11161 acgcaaatca ggtaacagaa ataaccagcg cagcccctga caagctgaca cctgttctgg
    11221 gatcagtgga taaggatacg cgtgaactca acctgctact tgtccagagt gccgatgaac
    11281 atctgcaggg tgtggtccgg ctgaacggca cattgtatcc ggcactggcc accccatcgg
    11341 ccgacaacag tcagctggtt atcaatgcgc tgaccgacaa aggtttacgc tttgcaggtt
    11401 atggtgaagc ggtaaaccac gatgctgaca gcacgaatcg ccccgcaccg gagcttatgc
    11461 agtttcatct gaaaacccgg gaagaaccgc tttttgctgc tgtctacacg ccggaaaaac
    11521 aacctgatgc gttgtaccgg aacctgggct tcgagcagtc atggcagcag tggtccaaca
    11581 gccaaaagcc ggaggacagg caggaaaaaa ctctgcatca ggatctctcc cacagccccg
    11641 gccgatgagc cggtaaggaa gccatatgaa catgcataag tctctggttg ccgtgctcgg
    11701 ccttatcctg atttcatcca cggcaagtgc tgcaacctgt cgtgcgggta gcgcggcaca
    11761 ggccggtagt aatacaggat atgagcgagc gcgtgcagct gcggatgcat ggtcacaacg
    11821 tgaaaacgat gtttcctcat ccctgcagtc ctgtctgtca cgaatcaaaa aaatcagcat
    11881 aaacctcccc cagttcccca gccttgacga cattctgtcg cagctggaaa accaggtctg
    11941 cgacgcggtc gtggacaagg tgaatgaaaa gctgccgggg aatattgatc cctggaagga
    12001 ctacaaccta tgaccgaaaa taccgagcca tcctcgccat cacatccggc acctgacccg
    12061 gaaatactga agagcacaat cagggctatg aagcagtcag aacagcgggc aaataatgtc
    12121 cctgcgctga taaaagcgct tttatgtacg ggcacatgtc ttcttatcag tattactggt
    12181 aacgccattc agtactggca ctcaacgaat gtggagcgcg aatatttcgc tacagacaat
    12241 ggtcgtctgg tcaggctggc accaacaagt cagccagcat ggagtcagaa tgatgcaatg
    12301 gcctttggca gccaggcact ggcgaccgca ttcaacctgg attttgtgca ctatcgttca
    12361 cagatctcct ccctgtctcc gcgtttttct gacgaaggtt ttgtcggata tgtgaatgca
    12421 ttacaggcat ctaacattct ggagacgatc aagaaagaga agatgaatct gaccgccacg
    12481 acaggtgctg gcgttctggt acgacaggga caaatgtccg acggcgtatg gttctggaca
    12541 ttccagtatc ctgtccgtat gcgtctggtc ggccagacaa ccagcaagcc agaacagtct
    12601 tttgttttcg aaatcaccat ccagcgtgtt gaccccagac tgaaaccatc cggtatggaa
    12661 atccggcaga tgatctcccg taacgccgga ccgaactcct gagataagta atatgcaatc
    12721 acgttattta ctgagcacac tgctcctggt gtgcagcgcc gccacgtctg ccgataacgc
    12781 aggatggcag aacgcacgca caccacaaac gacgaattct gccagcgatc acgttcagaa
    12841 cgcctcccaa aacaccggag gtgttccggc aacgctggta aaaggtgagc tgcctgcccc
    12901 cgggcaggcc agcccgctgg tacaggatgc ggcaaggctt gattctgagc tctccgccga
    12961 tgagattcgc tcactacgca gtctcatggc ggataacgag cgggccatca atgcgcccat
    13021 caccagtgtt gtaccccgta tcagctctct gacagttaat ctgtcccccg gtgccagcct
    13081 tcctcttgtt cgcacagcca tgaacaacct gagcgtggtg acatttaccg atatcaacgg
    13141 ttcaccatgg ccacaatccg atcctcctta taacgcggca ccaaaactct ttgatgtgca
    13201 gtacaacgag aacatggtga caatcacacc attgcggcca tgggcatccg gaaatatttc
    13261 tgtctacctg aaaggtctgt cggtaccggt catcctgaac gtaaccagtg gcgaaacaga
    13321 tacgccgtca tccagtcagg aaatggacag tcgtctggat ctgcgaatac cacggcaggg
    13381 accaaccagc ccggttgtct ccatcccgac ggataagatc gccctgcatg atgcaacatt
    13441 acaggcattt ctggatggta ttcctccccg ggacccttca gttaagaggc tgaaattcac
    13501 aggtaatgtc ccggatacaa cgatctggca acacggcgat gaccttctgg ttcgttcccg
    13561 ggcgatgttg cgcgatgaat ttgaacagac cttgtcctct gccgacggaa ctcatctctg
    13621 gaagcttccc gtcaccccac tactgacttt ctctgtgaat ggccagtccg ttcacgtgac
    13681 accagaactg gagtaatcat gagtgctgaa caggatgccg gtaaatccgg aaagaaactg
    13741 gctgccctgc ttggactggg cagcatcatc ttatttggcg gcggctatat cgcgttttcc
    13801 aaactatcag ggagtaacag cgatatgcag tcagccgtga acattaacag tgcagcaagc
    13861 ggcggaacgc gcagcgttac cgaaacgcct cactatcgtg aactgttaag agcggataat
    13921 gaacgagggg ccgcagctgc agcccgcaat aatcagacgt tcattgccag tcttccacag
    13981 gggcttgata ttccggacac tccggaaaag caacaacagc ctgcagcaaa accggaaaac
    14041 tatgctcatc gccaggcttc cggcacacca caggaggatc gagcagccag tgaaaaacgt
    14101 atggagcggc tgcaaaagct gattgtaaga ataaaagatc agcatcctgc cggtagtaca
    14161 ccaaccatag caacgactat gtggaataaa tctccggcag aaacaacagg gcagaatggt
    14221 acgcagcaat tcgcattaca gaatgccagc ctgtccacgc ctgtagcaga aaaagggata
    14281 caactgatac ctgctctgac ccgtataccg gcatatattg atactgcagt ggattctgat
    14341 aacccgtcat ccaaggttat cgcaaccatt cccgctggcc cctgggcagg ggcaacgctg
    14401 ttcagtccgg gagtcaaact ggtgggtaac ggcgtggaga tccatttcga ccggatgagc
    14461 tggaacggca tggaccttaa ggtcaatgca tatgctcaac gggaagacaa cctgatgtca
    14521 tccgtggcca gcaatgtgaa tacacgctgg tttaaacata tcattctgcc gtcagtgtta
    14581 ggaggtgtgg gcagtattgg cacactttat aaagatgcca acacgcaggt tattcagggt
    14641 aactatggca cggttaccgg acgcgtggga atgcccagtg gtgaagctgt tgcgggcgtt
    14701 attgccggtg gtatggcgga aagaggaagt cagatattaa cccgccaggc agaaagtgag
    14761 ccctataaac aggtagaagt gtatcagcat gaagtcgtat caattctgtt tgttgacccg
    14821 gtaatgacaa acgacgccag gtcatcttcc ctttccagtg gcatttcccc ttcagttaat
    14881 cgtacatcac aggcggaaca acgctcgcag gcacgaatgc agacagccat ggagcaaaga
    14941 aaagctgtga tgcaacgtcg gtatgatgaa caaccggaga caccataatg aaacctgaaa
    15001 cagaaataga tgaaaccggc agcttcggtg aacccgaaga gaaacccgcc cccttctgga
    15061 aacgttccgt ctgggggata tctgtagcaa catgggggct atgtgcagtc gtgttgctcg
    15121 cagccatatg gtatctgttc ctgagagcgc catcggaaac aggcatgcct ccgtttaatg
    15181 atgcggatgc tggcgtacag acctggcaaa ccacacaaga atcatcacct tctgttcagt
    15241 ccactgagac tatgactgtc cggacaggag aagatatgag ccagcttgcc cgggatgtaa
    15301 aaacagagct ggataaccgc gatgaaaaaa ttcaggcaac actgaatatg cttcacgaca
    15361 gcattaacaa gctgggcgaa gctataaaaa aggatgaaga atacgcacag gaaacgcgcc
    15421 gtcagctgga tgatatccgg agccgcctga acggaataat gacacagaaa agcgtcactg
    15481 aatcctcttc cacaccgcat ccagcaaaga aaaaaacgtc gtctgttctt aacggaatga
    15541 aaatcatgtc gatggaaact ggtatggcct ggattcgctg gcagggtagt acctgggctg
    15601 tcagagaagg tcagacgctg ggcaatgtgg tgatccagcg gattgatccg actacccgga
    15661 ccatcatcac cagtgcaggg acgttacgct gatgaacatg gatgccctga ccgccattga
    15721 gaactttgcc agcagtattt tcagtgccgg aatggatttc ctgtttacct ggggagaatt
    15781 catcggggtg atcagcatga ttaccctgtt tgcccgcgcc cgttcagccg gtccggtaaa
    15841 aatgtctccc ggaaaattta ttgcggggat gttaaccagc tgcatgctcg tatcactccc
    15901 ggcaatgatt aacgccggag gcgtacaaat gggattcagg gctgactcat ttggcccgat
    15961 tgcatatgta caacctcaga cgtttggcgc tgctgctggc gcagcaaatg cagttctgtc
    16021 gctggccaag ctggccggtg tgggatttgt catgaacggg atatccatct ggcgcaaggc
    16081 cggactcgat ggacataccg cactgagtgc cagtgaatcc gtcagtaagg gaaacgtcaa
    16141 atttattgcc ggcgtcctgc tggtctttat cgatcgtgtc ctcaatgccc tgctggcaag
    16201 cattggaatc gtcttctgat ttttttcacc ttcatataaa ccactgatat atccatttaa
    16261 ggagaaacta tgtcacgtat tcgtgtcgtg gcggcccgtc tgtacgccgc atttatccat
    16321 gcacaactgt ttgtaatgtc atgccggagc aaactgacta agtacctgct gctcattccc
    16381 atgatgttgc tgcccaaagc cgttcttgcc gatggagatc ttgccgatat ggttcgcaat
    16441 gttggtgaag gtgctaaaac agcacagtca tcatcaatca ctattgcgca gttcatcggc
    16501 gtaattctgt ttctcggggg cctgattggc ctgaaaaaag ttgggaaaca aggggggatg
    16561 ggactggctg gttgcattgt atctatcgtc attggggcgg tacttgtcgc cggacctgaa
    16621 atgatgagtc gcagccagaa acagttgggg atttcctcaa tttccattgg ctaatcttgt
    16681 ccagggcact ggcatttatc cggtgccctt cctattcggc aatacaagga ggcacatatg
    16741 tctgcctgga gcccggagca cataacgtta cgacgaacag aaaacggcaa tgtgattgcc
    16801 cacgacagtc gggacgatca ggaattcatt tttgcagaat gctgttccga tgaaatcgca
    16861 aacatcattc tggaagccgt gaaatgttac accagtacgg agcctgctca tgcaacacag
    16921 cattaaagac ttgtggttgt atccctttcc agaaattgat gtggttcata ctcaggaggc
    16981 actgttaccg gaaccggaat taacaacgcc cggtcgatgt atctgctgcc ggcaaaacgt
    17041 caggcaccgt ttcagactgg atgactcatg gccactcaga cagcttaccg acacaatcag
    17101 taacacacga gttcgtctta ataaggccac agaacatctg gttaagttaa tcaggcgtgg
    17161 tgaaccagtc gcaaccggca aaaaggaaaa gtacaacacg gcagtaaaag ccgctgaacg
    17221 ggcgcttgag catgcccgtc tgtctgccag acgtctttca ctccgccatg tacagaaagc
    17281 tgaaatcacc agtacagagc cgttgtcaga gaaagagcag gagctgtttc atgaagacgg
    17341 cccgccttac agcttatgtg ctttctgcca cgcctggcat tcactgaatg gttatgccgc
    17401 agcacagggc gttatggtgt ggctacccga ccttcatcca tccacggtcg tggcccttaa
    17461 ccgtcgttca ttacaggaag ttttcagtaa tgataagttc cgggtccggc gaggacggga
    17521 agcgctctct gcactgatgc aaaacagact ggcggttgag gataaattcc gaagtttccg
    17581 cccggccgat ttcgctgacg tttttcgtcg ctacccccct tccggtcgtt cgcctctgcg
    17641 tgaaaaaatg aacggtattg ccctgattct gacaccagat tcattcatca aaaaagaata
    17701 cgttgattga tttttcttca tgccactcca gcgcggggtg gcattaaaaa atcaaactgc
    17761 acaaacctcc ctcctgtaag tccggagttc cctcacctat gaacataaac cgtttaat
//
