LOCUS       NZ_CP011336            18052 bp    DNA     circular CON 01-MAY-2024
DEFINITION  Escherichia coli O104:H4 str. C227-11 isolate 368 shch plasmid
            unnamed, complete sequence.
ACCESSION   NZ_CP011336
VERSION     NZ_CP011336.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03856947
            Sequence Read Archive: SRR1980037, SRR1980038
            Assembly: GCF_000986765.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O104:H4 str. C227-11
  ORGANISM  Escherichia coli O104:H4 str. C227-11
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18052)
  AUTHORS   Beaulaurier,J.A., Zhang,X.-S., Zhu,S., Sebra,R., Rosenbluh,C.,
            Deikus,G., Shen,N., Munera,D., Waldor,M.K., Chess,A., Blaser,M.J.,
            Schadt,E. and Fang,G.
  TITLE     Single molecule-level detection and long read-based phasing of
            epigenetic variations in bacterial methylomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18052)
  AUTHORS   Beaulaurier,J.A. and Fang,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2015) Genetics and Genomic Sciences, Icahn School
            of Medicine at Mount Sinai, 1425 Madison Ave, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000986765.1-RS_2024_05_01
            Annotation Date                   :: 05/01/2024 02:09:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,400
            CDSs (total)                      :: 5,274
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 136 of 316
            Pseudo Genes (incomplete)         :: 195 of 316
            Pseudo Genes (internal stop)      :: 60 of 316
            Pseudo Genes (multiple problems)  :: 68 of 316
            Pseudo Genes (short protein)      :: 2 of 316
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP011336.1.
            Source DNA is available from Dr. Matthew K. Waldor at Havard
            Medical School (mwaldor@research.bwh.harvard.edu).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..18052
                     /organism="Escherichia coli O104:H4 str. C227-11"
                     /mol_type="genomic DNA"
                     /strain="C227-11"
                     /isolate="368 shch"
                     /isolation_source="stool sample from a 64-year-old woman
                     from Hamburg who presented with bloody diarrhea and did not
                     develop hemolytic uremic syndrome (HUS)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1048254"
                     /plasmid="unnamed"
                     /geo_loc_name="Denmark"
                     /collection_date="2011-05-18"
                     /note="also called BCG-1 (Russian)"
     gene            <1098..1322
                     /locus_tag="AAF13_RS33175"
                     /pseudo=""
     CDS             <1098..1322
                     /locus_tag="AAF13_RS33175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(1319..2755)
                     /locus_tag="AAF13_RS27380"
                     /old_locus_tag="AAF13_27375"
     CDS             complement(1319..2755)
                     /locus_tag="AAF13_RS27380"
                     /old_locus_tag="AAF13_27375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="WP_000527428.1"
                     /translation="MGNLMKKYDRGWASLEIGAALLIVMIIVAWGAGIWQDYLKTKGWQ
                     AEARLVSNWASAARSYIGKNYTTLQASSTTSTPAVITTTMLKNTGFLSSGFIETNSEGQ
                     RLQAYVVRNAQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATGALR
                     SWSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGS
                     NNLNNIGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITRN
                     SKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERTAV
                     AGASCSPNGLVGRDNTGAILSCQSGTWKTSGSLNGSYTNLGSHRGSFSGRNSGGSTLFI
                     YASGGNGGSAGGACANTSRLQGYVGGTLISVNASNNPAYGKTAFISFAVPAGTSYQITS
                     YPTENTSCGAGVFSVFGYQT"
                     /name="shufflon system plasmid conjugative transfer pilus
                     tip adhesin PilV"
     gene            complement(2743..3399)
                     /locus_tag="AAF13_RS27385"
                     /old_locus_tag="AAF13_27380"
     CDS             complement(2743..3399)
                     /locus_tag="AAF13_RS27385"
                     /old_locus_tag="AAF13_27380"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="WP_001193549.1"
                     /translation="MQTIVGISLSPVYILPLMFIFSIIGRTLLFRVRYFLSDTEHLWYK
                     THTAVLSGIWLYSIAVALIILSPSQLLYRIHAVLILSFMLQMAVTDALTGLLPGTFTRR
                     FLIAGILSQITTDIWWLRATEIVSAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITAWSG
                     LYNAIWSVLLGAGGFVLWHSTWCIKGHKEGPLGPWLCLGHVLLLLDNLYQPLWVI"
                     /name="A24 family peptidase"
     gene            complement(3384..3944)
                     /locus_tag="AAF13_RS27390"
                     /old_locus_tag="AAF13_27385"
     CDS             complement(3384..3944)
                     /locus_tag="AAF13_RS27390"
                     /old_locus_tag="AAF13_27385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_000014005.1"
                     /translation="MSGGKVPLRLISLILLCSLIYPARASYIAPKQIWTNKWDNCFAAA
                     GARYQIEPLLLKAISAGESSLKPGAININKDRKTGKASSTDYGLMQINSTHIPKLIKMG
                     VIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYANKV
                     WRIYRDMKGICLPGQGGRQCRQS"
                     /name="lytic transglycosylase domain-containing protein"
     gene            complement(3954..4568)
                     /locus_tag="AAF13_RS27395"
                     /old_locus_tag="AAF13_27390"
     CDS             complement(3954..4568)
                     /locus_tag="AAF13_RS27395"
                     /old_locus_tag="AAF13_27390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="WP_000908227.1"
                     /translation="MLIQNIDVNALKKNEKETSHDKGWGIMEQGAIALVVIIVLALVFG
                     GLYMLRSRTGVANESSNIQTIITSTQGLLKGSDGYTFTSGTKMTGALIQMGGVPKTMTV
                     RGTPSSGTATLYNSWGGAVTVAPASTSGFNNGFTVTYEKVPQDACIQIATQISRTGLTN
                     GITLNSTTHNDGKVTTEEASAQCTADNGSTGTNKLIFTING"
                     /name="type 4 pilus major pilin"
     gene            complement(4585..5671)
                     /locus_tag="AAF13_RS27400"
                     /old_locus_tag="AAF13_27395"
                     /pseudo=""
     CDS             complement(4585..5671)
                     /locus_tag="AAF13_RS27400"
                     /old_locus_tag="AAF13_27395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722224.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /name="type II secretion system F family protein"
     gene            complement(5684..7237)
                     /locus_tag="AAF13_RS27405"
                     /old_locus_tag="AAF13_27400"
     CDS             complement(5684..7237)
                     /locus_tag="AAF13_RS27405"
                     /old_locus_tag="AAF13_27400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="WP_000362206.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLNLYP
                     DIIPKVVSLSELQEAGQDDENKGPKERIIHLKDLADVSESEKKVLSYFETARKLGASDI
                     HFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQDARL
                     SPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLNVMLR
                     RPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTPIICDK
                     SDEEAVKLAWSRAISSAMRLDPDAIMEGEMRDLVSMMSTTYAAQTGHIVLTTLHTNSAL
                     GIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWEKRVPELSEDDRAYLER
                     HCNKDSLCSTDNIWFRNPHGCSDCNHDVIINGRKRGEIGKGLTGRTVIAEVIEPDNRLF
                     QILKTRGKVAARKYWLENMHGISRVEHLLRRINEGLVDPLEADRIIPLDEDERLSIDDA
                     "
                     /name="ATPase, T2SS/T4P/T4SS family"
     gene            complement(7248..7700)
                     /gene="pilP"
                     /locus_tag="AAF13_RS27410"
                     /old_locus_tag="AAF13_27405"
     CDS             complement(7248..7700)
                     /gene="pilP"
                     /locus_tag="AAF13_RS27410"
                     /old_locus_tag="AAF13_27405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /GO_process="GO:0009297 - pilus assembly [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="WP_001247337.1"
                     /translation="MRPGKSLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQAK
                     VQGAQLQKQLEESDVNLSSETVSGFSGMTASLPSVSEQPTSKNRKELPVIMEINGKDKR
                     LHAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
                     /name="pilP"
     gene            complement(7687..8982)
                     /gene="pilO2"
                     /locus_tag="AAF13_RS27415"
                     /old_locus_tag="AAF13_27410"
     CDS             complement(7687..8982)
                     /gene="pilO2"
                     /locus_tag="AAF13_RS27415"
                     /old_locus_tag="AAF13_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="WP_000752782.1"
                     /translation="MADEDINPVVLLADPKVNHRVWAACLKWTPVVKKQRVPSHHKHKY
                     HVKPRRLTSLKVTVGSRNSRGKISRLTGTGILTRPERNHYFSLALAFCSWVRNGYGVFR
                     YSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLAMNEEPPEKWQVVSSLEHPDNW
                     ESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAAGAGTVFWMTQPEPDVGPTAEEIA
                     ARARLQFKKPEPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTCTPETF
                     TLIYERQPGGTIEGFLARSKEIFNVIPDFNLKDGARLASVTRLLPSLPRRDEAVPAPSE
                     QLMRVFTWFQKKQLTPAINEIAIPEPLPGNNGEPAPVQKWKEYQFSLSTPVNPDALFPL
                     FQDTGVRLSNIHFELNGGTFSYSSEGHIYASR"
                     /name="pilO2"
     gene            complement(8975..10657)
                     /locus_tag="AAF13_RS27420"
                     /old_locus_tag="AAF13_27415"
     CDS             complement(8975..10657)
                     /locus_tag="AAF13_RS27420"
                     /old_locus_tag="AAF13_27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0009297 - pilus assembly [Evidence IEA];
                     GO:0009306 - protein secretion [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="WP_000748144.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSAH
                     AREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQELG
                     QRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTSTQP
                     LTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSSSSSV
                     SSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGRYWLSA
                     SSSTLTVTDTPAVQEAVTRYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDWNLVYKS
                     LHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDVSVVTSQE
                     STVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLLPLIQKTGN
                     LQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLVVTGFDQNNT
                     TTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
                     /name="PilN family type IVB pilus formation outer membrane
                     protein"
     gene            complement(10671..11108)
                     /gene="pilM"
                     /locus_tag="AAF13_RS27425"
                     /old_locus_tag="AAF13_27420"
     CDS             complement(10671..11108)
                     /gene="pilM"
                     /locus_tag="AAF13_RS27425"
                     /old_locus_tag="AAF13_27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="WP_000539806.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQILM
                     IANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETLRL
                     KSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
                     /name="pilM"
     gene            complement(11108..12175)
                     /gene="pilL"
                     /locus_tag="AAF13_RS27430"
                     /old_locus_tag="AAF13_27425"
     CDS             complement(11108..12175)
                     /gene="pilL"
                     /locus_tag="AAF13_RS27430"
                     /old_locus_tag="AAF13_27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="WP_001360287.1"
                     /translation="MKKNYLTLILPSLLSGCAGISGGNNVAPSDTPALVFVDGQISESA
                     GVLALTQQRLSPPKITPPPRVSVPETVKTAQPVSESRPLPSPTISQNFSRLIITGKPGS
                     TPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLNRLL
                     EEQHLWGHLDWDNKTLTVTTSAAAPAQDTATTISPAAMVTTSTSPESSTTANSQNKPRN
                     PFRGNSVSPSTPAAIPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLRENIIKWA
                     EETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKPLYAQAS
                     RMQCIITVSDTRDGR"
                     /name="pilL"
     gene            complement(12504..13097)
                     /locus_tag="AAF13_RS33180"
     CDS             complement(12504..13097)
                     /locus_tag="AAF13_RS33180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001387475.1"
                     /translation="MKNNNAPLYLWLLWCTLCLYITTPWQYLRYPYTDNLSLPYMLYFC
                     VVVVICIFPLLFRCVLLIVRSASKYDFTFFVKKKKGSKKSTIHLSPYADTRCPKSMRKH
                     WDVLLSLISKAINNDEIIVMHSHLFTPARINKLLKRLESYDLSFSVITYPRKTSLYEKL
                     SIPFIYLLFQWSFPHLHSDGMTVVIRPLQTHHTN"
                     /name="hypothetical protein"
     gene            complement(13222..13477)
                     /locus_tag="AAF13_RS31610"
                     /pseudo=""
     CDS             complement(13222..13477)
                     /locus_tag="AAF13_RS31610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /name="PilI type IV pilus biogenesis protein"
     gene            complement(13649..14331)
                     /locus_tag="AAF13_RS27435"
                     /old_locus_tag="AAF13_27430"
                     /pseudo=""
     CDS             complement(13649..14331)
                     /locus_tag="AAF13_RS27435"
                     /old_locus_tag="AAF13_27430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /name="conjugal transfer protein TraC"
     gene            complement(14585..15118)
                     /locus_tag="AAF13_RS27445"
                     /old_locus_tag="AAF13_27440"
     CDS             complement(14585..15118)
                     /locus_tag="AAF13_RS27445"
                     /old_locus_tag="AAF13_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="WP_001372168.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITRK
                     IRRADSRCCYRERIFAIFPGYFFILANFDIQPVSALRRHSAFIDFVKFGGEIKPVNKDI
                     VDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLELVSN
                     AEHHGFMERLVNIP"
                     /name="transcription termination/antitermination NusG
                     family protein"
     gene            complement(15561..15849)
                     /locus_tag="AAF13_RS32925"
                     /pseudo=""
     CDS             complement(15561..15849)
                     /locus_tag="AAF13_RS32925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /name="conjugal transfer protein TraA"
     gene            complement(<15877..16046)
                     /locus_tag="AAF13_RS32445"
                     /pseudo=""
     CDS             complement(<15877..16046)
                     /locus_tag="AAF13_RS32445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736486.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /name="ash family protein"
     gene            17004..18045
                     /gene="repA"
                     /locus_tag="AAF13_RS27455"
                     /old_locus_tag="AAF13_27450"
                     /pseudo=""
     CDS             17004..18045
                     /gene="repA"
                     /locus_tag="AAF13_RS27455"
                     /old_locus_tag="AAF13_27450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /name="repA"
     Typing          17005..18045
                     /name="IncI-gamma/K1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.469"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agctatttca agcaagtaat cttcataaag taggacgaat actgtgtgta gtttttggct
       61 tgccaagtca tctcgccgtt agagtcaggc atgtcaatag gaacgacttc tgtttctgcc
      121 atttcacgcc cgtcaatacg ataggtgtta atacacagtt taaacctccc taagacctgt
      181 gtgtttttgg gatataacgt aaatgttttt atattcgtcc atatcgacgc tgatgatgat
      241 ttccacgtcc cggattggca cgtaggccag tgccaatccg gtacttggcg taaaagcaat
      301 tctggtagta cggtgataac tggtcgtata gcaaatggtc agcaaatacc gctgccaaca
      361 gggttttctg ccagtcagtg ctcatggagt gtgagcaatg cagaaaaccc tcaaggttgg
      421 aagccgaatt actttgcagg atcagttgca acttatgatg caaatcgaat tgttaaatgt
      481 ggtttttatg atgaatacaa tttccataaa gggacattta gagctgattt aaccggaaaa
      541 tgtagttatg tcgtcgcgtg tcagaactga gattaaattg ctggcgctgc tggcctacgt
      601 gccaatccgg tacctggggc gctcctaaaa ttcaattcac aacgcagacc tacaacatag
      661 ctaaaaacac tcgtaattta aggctcgggg tccatgcata ttgctcatgg acttacctga
      721 atggctcacc ctttggtggt tttcaacagg tatattccga ccaaaacaac gtttggtatg
      781 tgagtaatta tgcttgggga aattatgagt ctggtgggac catatcagtc acatgcctca
      841 atcttcctgg tgctggagct taattaaagg ggcaacagta ggcatcgaca tgttcctcat
      901 caacaccatc accaataccg tacatgcgtg ttccagtcat aatgtatcca gtcggacatt
      961 gaatcggctt gtaatagatc gaaccaccac tcttaccgcc gataaagtga ttcattgcta
     1021 cggatgattt gtaccaatta catgctgagt aattaatttt attaccccct gaccaccgac
     1081 cggattggca ctggcctacg tgccaatccg gtacgtgggg gacaataggt ggaaaactca
     1141 aagttactca gctttccacc acaggttatc tggggcaatt cgacttctgt gccattgcca
     1201 gaatgggcaa cgcagaggat gcccactact gccaggtagt tgagagccca gcaggttcac
     1261 gcaaatggta caaatacgag cataagacag gttgtattgc gtcgtgtgta acgctcaatt
     1321 aagtttgata tccaaatact gaaaataccc cggcaccaca tgatgtattt tctgttggat
     1381 aggatgttat ctgataggaa gtacctgcag gtacagcaaa gctgataaag gctgtttttc
     1441 catatgcagg gttattactg gcattcacgc taattagcgt cccaccaaca tatccctgca
     1501 gtcgggatgt atttgcacat gcacctccgg cagatcctcc attacctcca gatgcataaa
     1561 taaacaatgt actgccccct gaattccgcc ctgagaatga acctctatgt gaacctaagt
     1621 ttgtgtaaga accattgagc gaaccagaag tcttccacgt accggattgg cacgaaagta
     1681 ttgcccctgt attatcgcgg cctacaaggc cgttaggcga acatgatgcg ccagcaacgg
     1741 cagttctttc cagttgtaag tattcaccag tataaaggcg accatcagcc cgaacagtac
     1801 cgcctttcac ctgaccgccg gtatagatgc ctttgttgtt tacacttcga acccatgatc
     1861 cgtcggacat ataaaatccg ccaccgtgag tttcattgag ccagcctttg ctgttacgag
     1921 taattaacca accattatta cttctgatat caccgccagc ggtgacattt acgtcaaagt
     1981 ttccgctatt gccttttacc tgaccgctga aagtgccatt tacaccattc acattgccgc
     2041 tgaaattacc agtctgggca tttactgccc caatgttatt caggttattt gatcccatat
     2101 caatggccgt gtgcattttg tttaagtcag ggcgaccatt gacctggaat ctgtaaagac
     2161 gatcagtgtc ctctgctgca ccactaagtt catctgtcga taacagtacg gcaatatgcc
     2221 cgttaccgct tttggcacca taattactta aagctactga ccaggaacgt aatgcacctg
     2281 tggctgtttt gccgtcctgg atatatccac caagaccagt ggtaatatcc ttagccatct
     2341 ggataagtgc tttcactgga taaggggtgc caccactgga tacaaccatt gcctgtagta
     2401 attccgggtt ttgggcgttt cgaactacat atgcctgtaa ccgctgcccc tcgctgtttg
     2461 tctcaataaa gccgctggac aaaaagccgg tatttttcag catggttgtc gtgataacgg
     2521 caggggttga tgtggtacta cttgcctgga gagttgtgta gtttttccct atataagagc
     2581 gggctgcact ggcccagttg ctgacaagac gagcttcagc ctgccaacct ttggttttta
     2641 aatagtcctg ccatatgcct gctccccagg caactatgat cataacaatc aataaagccg
     2701 cgccaatttc cagcgatgcc cagcctcggt catatttttt catcagatta cccatagtgg
     2761 ttgataaaga ttatccagta gcaaaaggac atggccaaga cacagccatg gacccagtgg
     2821 gccttcttta tgtcctttta tgcaccatgt tgaatgccat aagacaaatc ctcctgcgcc
     2881 cagcaacaca ctccatatgg cgttatatag ccctgaccat gcggtaatac ctgcaataag
     2941 ccacaggtct cctgtgccaa tattaagacg atggcggtta accagtttat gcagacagaa
     3001 taagacaatt gcggcagaga caatttctgt tgcacggagc caccatatat cagttgtgat
     3061 ttgtgataat attccagcga ttaaaaaacg cctggtgaat gtaccaggta agaggcctgt
     3121 tagtgcatcc gttactgcca tttgcagcat gaacgaaaga ataagaacag catgaatacg
     3181 gtagagaagt tgggatggcg ataaaataat taatgccaca gctatactat atagccatat
     3241 tcctgacaga actgcagtgt gtgtcttata ccagagatgt tctgtatcag ataaaaaata
     3301 acgaactctg aataacagag ttcgtccaat aatggaaaat atgaacatca atgggaggat
     3361 ataaacaggg gagagactta tgcctacgat tgtctgcatt gtctgcctcc ctgcccggga
     3421 aggcatatcc ctttcatgtc acgataaatt ctccatactt tattagcata ttgctctctt
     3481 gtttcatgtc tgtccttacg gaatccggca ttgtatgatc caaggcaatt ccagctgacg
     3541 ccacatatct gaaaatgtct tgccagtatc cagcttccta tatggatatt aagacatggt
     3601 ttggtaatta aatcctcaga ctttttgatt acgcccatct ttataagctt tggaatatgg
     3661 gttgagttta tttgcatcaa cccataatcc gtactactgg ctttgcctgt ttttctgtcc
     3721 ttatttatgt taattgcgcc cggcttcaat gaagattccc cggcagaaat ggccttcaac
     3781 aataatggtt caatttgata gcgtgcgccg gcggcggcaa agcaattgtc ccacttgtta
     3841 gtccagattt gtttgggggc gatgtatgaa gctcgggcgg gataaataag agaacaaagc
     3901 aggattaatg aaataagtct gaggggcact ttacccccgg acattaaaac tccttagcca
     3961 ttgatggtga agattagttt gtttgtaccg gtgctgccat tatcagctgt gcattgcgca
     4021 ctggcttctt cagtagtgac ttttccatca ttgtgagtcg tactgttcag ggtaataccg
     4081 ttagttaaac cagtacgact gatttgtgtg gcaatctgga tacaggcatc ctgtgggact
     4141 ttttcatagg tgacggtaaa accattgttg aaacctgatg ttgaagccgg agcaacagtt
     4201 acggcaccgc cccatgagtt atagagcgtt gctgtacctg atgatggagt accacgcact
     4261 gtcatagtct tgggtacgcc acccatctga atgagagcac ccgtcatttt ggttccactg
     4321 gtaaacgtat atccatcgct accttttagc agcccctgag tacttgtgat gattgtctgt
     4381 atgttcgaac tttcatttgc cacaccggtt cgtgaacgca acatatacaa tccaccaaaa
     4441 acaagggcaa gtacgataat tacaacaagt gctattgcac cctgttccat aataccccag
     4501 cctttgtcat gagacgtttc tttctcattt ttcttcagag cgttaacatc aatattttgg
     4561 attaacataa atacctcgtt ttatttaaag attacccata ctgttgtcgc caatagactg
     4621 aatgtctaca acggccatga ccagtaacag gagtgacatg acaagaaata tcagcataat
     4681 taaacgcaca acagctgctc gtttttttac tcgctccagc gtttgtacaa gccaccgttg
     4741 gccataattg ccgattagct ccgttgcgcc atctccctgt aataaagaca ggaagttggc
     4801 tgcttccctg gatgggaact gataaccgca ctgacgcaaa gccagcccaa ggtgatcccc
     4861 ctggcgtacg cggtaaataa tgctgtcaag gcgtgtgctt aaccatggtg atgcaaagtc
     4921 ctgaagaacg ttcagtgagt tgagcgttgt cattttggct ttcagaagtg cagccatatt
     4981 caggaggaat gctgccccct gtatatcctt ataaatactc catggcatta tattgtcagc
     5041 caaagagcgg cttttccagt ttttcagaga ccatgatatt acacctgtga ccactgcaaa
     5101 aagaacggca catatagcgc cgtaattatc gacaaacaaa gataatccat agagaaaacc
     5161 aagagcacca ctccatgaat caggggagct tattttgctc agtacaggta ttagctctgt
     5221 attaagaaca tataatgtcc ccgtcatcat gattaacaac cccaccgggt aaatagccat
     5281 ttgccatatg gcctgatgga gttgcttttt ggcatctgta agtgtggtgg caaattgtaa
     5341 tgcatccaca atggaaccac ttcttattcc ggcactgatt actgcagcct cttcctgggg
     5401 aacccataaa ccaagagtat attccagaga gttttcgcca ctgttttcac gaagagactc
     5461 aatacagtct gtggccagtt cagcaaaggg atgccatttt cgtccaaagt ctgtccaggc
     5521 atcccgcatc tgttccagtg cggtctttaa tggctgttta ttttccagca gaaaacgcag
     5581 agcttcgtaa aactgcaccc tgtagggggc gctgaacgtt ttttctgaca atgaaacgtc
     5641 tcagccgttg actgaaattc atttcacgca tgttgttctc ctgttaggca tcatcgatgc
     5701 tgaggcgttc gtcttcgtcc agagggatga ttctgtcggc ttcaagaggg tccaccaggc
     5761 cttcattgat acggcgaaga agatgctcta cacgactgat gccatgcatg ttttcaagcc
     5821 agtatttacg tgctgcaact ttgccgcgtg ttttcagaat ctgaaataag cgattatcag
     5881 gttctatcac ttcagcaatt acggtcctgc cggtgaggcc tttacctatt tcaccgcgct
     5941 ttctgccgtt aataattacg tcgtgattac agtcggagca tccgtgagga tttcggaacc
     6001 agatattatc tgtactgcag agtgaatctt tattgcagtg ccgctcaagg taagcgcggt
     6061 cgtcttcgct aagttcaggt actcgctttt cccatggaat acggcaggag ggacacagag
     6121 ttggtacaag acgctggctg atcatcccaa tcagtaattg cgcatcgcaa ataagatcgg
     6181 cattcatacc catcgtaatc atccgctccg gtatacccag agctgagttt gtatgtaatg
     6241 tcgtcagaac gatgtgaccg gtctgagccg cgtaggttgt tgacatcatc gagacaagat
     6301 cgcgcatttc cccttccatg atggcatcgg ggtcaagtcg cattgccgat gaaatagcac
     6361 gactccatgc cagtttgacg gcctcttcgt cggatttgtc acagataatc ggggtttgtg
     6421 ttgcccccag aatctgtcct tccagcggat cttcgatcgt cagcaggtgt cgcccctgat
     6481 tatcgtcaag atatacacgg caggcactgc gtaacgttgt tgatttacct gaacctgttg
     6541 gaccagacag aactattttc ccttccgggc ggcgcagcat gacgttcagc aaccggattt
     6601 gctccggtat aaagcccagt tgcttaaacg tgggaacttt gtctccgtca tcgggtatca
     6661 ggcgcataac tgcaatcaga ccatctcccg taggtctgtg actgtatcgt gcaccaaata
     6721 ttcctatttt tcgcattaat tgtggggaca ggcgcgcatc ctgttccctt tgagggaaga
     6781 aactggtttc agtcacatca gccattgaca gaatggctgt tgcgcaaagt gaataaccca
     6841 acgcaggttg atcttcatct acagtttgca gttcaccgaa tattctcatg cgaactttga
     6901 aaatagactc tgaaattaaa aaatgaatat ctgaagcacc aagttttctg gccgtttcaa
     6961 aataggagag aactttcttc tcactttcac tgacatctgc aagatctttc agatgaataa
     7021 tccgttcttt cggaccttta ttttcatcat cctgaccggc ttcctgtaac tcagacagcg
     7081 agactacttt tgggatgatg tcagggtata agtttagcag ttcctggaga ttactctgta
     7141 cgtcaggtct tgttcgctga tttgtttcga tgagaatttc atgggtgtct ccccgggata
     7201 taaaaaggag agcatccttc aaattatatt tgtccatatc acatcactca gaaagtaagg
     7261 gtggtgccat cacttaacgt gacgccagac aatgagatcg acttcaccgt gacggatgta
     7321 cccggaagct ggctaccagt ggtgacgctt gtctgacgtc catcagccat gcgaagaacg
     7381 gcatgaagac gtttatcttt gccgtttatt tccataataa cgggcaattc ctttctgttt
     7441 ttagatgtcg gctgctctga aactgatggc aaagaggcag tcataccaga gaaacctgaa
     7501 acagtttcag aggacagatt aacatctgat tcttcgagtt gtttctgtag ctgtgcgccc
     7561 tgaacttttg cctgtaaaag aatattccgg ttttgttgtg cttcaagttc tcctattgtg
     7621 acaagaggtt gtgttgttgc aaatgaaaaa ccggagaaga aaaatatgga tggaataatg
     7681 agtgatttac ctggacgcat aaatatgacc ttctgaggaa taactgaaag taccgccgtt
     7741 taattcaaaa tggatattgc tcagacgaac gccggtatcc tgaaataaag ggaacaacgc
     7801 atcaggattt acaggcgttg ataaactgaa ctgatattct ttccatttct gaacaggggc
     7861 tggttcacca ttgttacctg gtaatggttc cggtatggct atttcattaa tggccggcgt
     7921 taactgtttt ttctgaaacc atgtgaacac ccgcatcagt tgttctgagg gggcagggac
     7981 tgcctcatcc ctgcggggca gggaaggcag tagccgggtt acagaagcca ggcgtgcacc
     8041 atctttaagg ttaaagtcag gaataacatt aaagatttct ttgcttcttg ccagaaagcc
     8101 ttcaatagtt cctcctggct gtcgttcgta aatcagtgtg aatgtctcgg gagtacatgt
     8161 tccaccggta agtttccagc cttctagcgc gaccggtgat ggttttcgaa ggtccgcgca
     8221 ggctttcaga aaatcactga ttacaggttg tgacgcccat ggatgcggga gctccggtgg
     8281 tggttcgggc tttttaaact gtaatcttgc ccgtgcagct atctcctcgg cggtcggtcc
     8341 gacatcaggc tctggctgag tcatccagaa tactgtgcct gcgccggctg ctactaaaaa
     8401 cagtacggca ggcagtgtga atttgctgcg attgccgaca gtaagtttgc aacggcggag
     8461 gtctgctgat gataaccggg taataatact ttcccagtta tcaggatgtt ccagagacga
     8521 tacaacctgc catttttcgg ggggctcttc gttcatcgcc agaaaaaggc tgaccttttg
     8581 ggcaacatcg gcatcatttc ctgataagtc agccataacg gcaggctggc cattaatgga
     8641 agcaaggaat aagagctcct tatcgctgta ccggaagaca ccgtatccat ttctgaccca
     8701 tgagcagaaa gccagtgcca gggaaaaata atgattgcgt tctggcctgg tcagtattcc
     8761 tgtgccggta aggcggctga tctttccacg ggaatttctg ctgcctacag tcactttcag
     8821 ggaggtaagc cgtctgggtt ttacatgata cttgtgttta tgatgtgagg ggacgcgctg
     8881 tttttttacg acaggagtcc atttaagaca ggcagcccac acgcgatgat ttactttagg
     8941 atctgcgaga agcactaccg ggttgatatc ttcatcagcc attattgcct cctgacggga
     9001 aagtcggggt aatgatgatc acaagcgtgc tgcgttcatt tttcccggtt tgtgaaccac
     9061 caaataatgg atttgctggc gtaaatgtac cggctttact tgtcgtcgta ttgttctgat
     9121 cgaaaccagt aacgacaagt gattgccctt ctttcagatt gaccttctgg ctcagtgaac
     9181 gcagtttggt atatggcatt tcgatgtaac tgtttccgtc ttttgacgta aagctacgga
     9241 tagttggggg atcagacaga ttaaagttca tctgcaactg gagattgcct gttttctgaa
     9301 ttaaaggcag cagggtcata ttgaatccag tggtgatcat gcccggtgtt aatgttgttg
     9361 ttgctcctac atccgtagtc gtagttgttg ctgactgggc gacgtaaact gtctgatctg
     9421 ccatctggat aggtaccggc gtcaggtttg tgacagtgct ttcttgtgaa gttacaacgc
     9481 tgacatcgcc ttgttcactc agtgctttaa tcagaaggct ggaaccgctg aatttggcgg
     9541 cattccctgt tgccgtatcc agaattgata cgcctgcaga tgtagcgcct gtaaaatctc
     9601 cgcttgcatt gttcaacgtt gctccggcgg aatgtagcga tttataaaca aggttccagt
     9661 ccagaccgaa ctgttcgttt ctggtattgc tgacgctcag aacctgtacg ttcagggcta
     9721 cctggcggtt cataatactg ttttgttcgt ctacatatcg tgtgacggct tcctggacag
     9781 ctggagtatc agtgactgtc agcgttgagc tcgatgcaga taaccagtaa cggccttttt
     9841 ctggtgtcag cattgcttca atagttttcc ggatatcttc atacagatcg tattcctgac
     9901 caacggttgt gctctgagag gacgttgcat caccggatgc tgagttatcc tggcctcctg
     9961 ttgcccccat tgtgcttgtt gagccagagc tgacactgga actgctgctg gttttggtgt
    10021 tcagcatatg aagtggatac gttctggttt cagtcagata gaatacaatc cgaccattat
    10081 ccatgcgcca gtacagacca ctccggctgg ccatcagatc cagaagacca ttgatatctc
    10141 cctgccacat gaggttattc agcgttaatg gctgagtgga ggtagtcatt gttgtgctgc
    10201 ccaggctgct taacggtaga cgtccatttt catctggtgc tggtagtgtt cctgtcatct
    10261 ggcgggtagc gcctccttca agagttgaat tggctgcgtc aggcgtgatg atcacaggga
    10321 tgccgcatac ggcagtaata cgttgcccca gttcctgcag agtgatctct ccttttcttg
    10381 cctgcgtgat gtagcaggct ggagctgttt gttttttctc tcttgatacc tgagcgaccg
    10441 gaacagggtt tatccattga ttatcgagcc aggtaagagc ctgcgattta cgggccgaaa
    10501 gggctgatac cttttcccgt gcatgtgctg agtcttcctg tgcttttttc tgcattttgt
    10561 tgatttcgct gaaagtacat ccggagatgg aaagagcgac ggcgatcatg caggggagca
    10621 ccgccagctt cattgaacgc tggtgtgatt ttttcatgaa tatatccaga ttagttaacg
    10681 tagacgacag aaccagcagt aatgcctgcc gggggtgtca gaccggttgc agttcctgat
    10741 agccaggtga gttgcccgtt ttggaagatc cctattagtg cagaaccacg actttttaaa
    10801 cgcaaagttt caacaagtcc tggttgttca ggcatccata cccacagacg cccctgttgt
    10861 aactgatgtt taattcggga gtccggagtg acagggagag ccagtttatc tgaggaaatt
    10921 actccgtcct gctggcctga tacgtatctg atatcattga ttctgttagc aatcattagt
    10981 atctgacttg cccatacaga cgcacttgca tattgctggg cgtttgtggt ttcagacatc
    11041 ttctgattct ggtagctacc agtgataatc agaatggtca ggccgacggc catgaccagc
    11101 catcccatta gcgaccatcc cttgtatcag aaactgtaat gatgcactgc atgcgagaag
    11161 cctgtgcgta taaaggtttt tgcgcagtcc gatataactc aaatacctgc tccatggctg
    11221 attcaaatga tccccggaac atcaaaggcg catcaagacg gtaatcggtt acagaaagtg
    11281 gccatatgac catccagtgc gttgatgcca tgctttcgca ttttgtttct tctgcccact
    11341 taataatgtt ttcccttaat gtagtgcctg ccggcgcacg ccactcttta ccctgagata
    11401 ccggtttgac aggtgtcccg gtcatgagtg ggatagactt gactgtagag ccggtcggag
    11461 tcgggattgc tgcgggcgta gacggagata cgctgtttcc cctgaatggg tttcgtggtt
    11521 tgttctggct atttgctgtc gttgaagact ccggggaagt ggatgtggtt accatggctg
    11581 ctggcgagat agtggttgcc gtgtcctgtg ccggtgcagc agccgatgtt gtgacggtca
    11641 acgttttatt atcccagtcc agatggcccc agaggtgttg ttcttccagc aaccggttca
    11701 gagaacgggt ccactgatca ttggcagacc atgaaaccag tttggtattc agtttgggcg
    11761 tggctgcatt ctcttgtctg aatttccatc cttccggtag taatgttttt atccactgac
    11821 tgacggtccg gttacgggcc gtcggcgata gtgttacagg ggtgctacca ggcttgcctg
    11881 taatgattaa ccgggagaaa ttctgactta ttgttggtga aggtaacggt cgggattcgc
    11941 ttactggctg tgctgttttt actgtctctg gcacgctgac tctcgggggc ggagttattt
    12001 taggtggaga aagtcgttgt tgcgtaagtg ccaggacgcc agcactctca ctgatttgtc
    12061 cgtcaacaaa aaccagagca ggcgtatctg atggtgccac gttattgccg ccggaaattc
    12121 cggcacagcc ggacaataat gacggcagta taagtgtcag atagtttttt ttcataacgg
    12181 agactccgtg ttcgtatata cgtgtttgtt agttaatgcg ggctttaagt aagggttcat
    12241 tattttatgt gcaattggat gggtaatgta taccggtatt gagagaatta ctcctgaatg
    12301 tcccgtttta gtcgcattca gaagtctgct tgggtcttta atattgttga ttagtatttt
    12361 cattattcta tcttttgaat tttaatgttg catgttattt taaaggtgag gttgtggata
    12421 gccaattaag ccagtgtttt gggatttttt gtgaaaataa aaatcctgct tatgagcatt
    12481 ctttttatat ctaaatgtat tttttaattg gtgtggtggg tctggagagg acgaatgacg
    12541 actgtcattc cgtccgaatg caaatgcggg aaagaccact gaaacaagag atatataaaa
    12601 ggaattgata atttttcgta cagacttgtt ttccgtgggt atgtaataac tgaaaaactc
    12661 aaatcataag attccagtct ctttaaaagt ttatttattc ttgctggagt aaagagatgt
    12721 gaatgcatga caatgatttc atcattatta attgcttttg atatcagaga aagcaataca
    12781 tcccagtgtt tcctcattga cttcgggcaa cgtgtgtcag cataggggct aagatgtata
    12841 gttgatttct tgcttccctt tttcttcttc acaaagaatg tgaagtcgta tttagatgca
    12901 gaccttacaa taagaagtac acatctgaaa agtagtggga aaatgcaaat cacaaccacc
    12961 acacagaaat ataacatata aggcaatgat aagttgtcag tgtatggata acgtaagtat
    13021 tgccatgggg tagtgatgta cagacaaagc gtacaccaaa gcaaccagag atataatggt
    13081 gcgttattat ttttcatgtt ggctgaggca ggcgactaaa ataacagact aaggaggatt
    13141 aactgtttaa ttctttggaa caaaaaaatg cagttcctga tcatcacaca ccattgaata
    13201 aacattttgc ttcggttgcg ttcatgatag ccagtcctgt agatgctgat ggcgggagct
    13261 gctgcccttg tgccagcggc agtggctgcc gggagcacgc tccagtcgga acagaaatcg
    13321 gttgtcgcgt agtaccacca cgagacagtt atccggcgtg cagaaacgtc gggcaagctc
    13381 atcggggtct gtcggtgttt ttcgttgaaa acagcctgcg tttgcagtgt gcgtcgacga
    13441 ccagtatttg cagtcgtccg ggatgttgct gtggcatcaa agactccttc cggaacggga
    13501 aaagaagcag ctgtaccgtc ataactgcac gtaccggttg cgaaaaaacg gcacaagcga
    13561 gtagtgacag aaagaatgtc ggaaacggca ggaagggcgg tacaggctgc gaagactgga
    13621 aacggggtat gccggggatt acacatcctc agtgtaatgt gatagtcgtc tgctcctgtg
    13681 ttgcacccca gacatcttct ggcaagctgg gcagcccgat aaatagggtg tgaaagaggg
    13741 tttctctgcg ttcgatcccg aattgatgtt cgtatcctct caccacctga aatccttcgt
    13801 cggagaatat taactctgcc gtttccagaa gctgcatagt tttgcggtgt agtggagtga
    13861 tcagctgcaa cgtaactggt gccctcatat cgcctgtcgg tagaaaacac tcttcggtgc
    13921 gacttagttg tagcagatgg cgtttcccgg aacaggcccg ttcaatggat tcattgaaca
    13981 ccatctcagg ccatgggcag acataagtgc gccagcctgc tttctgtaat ttacccagcg
    14041 cagcccgaaa acgaaacaac ggacattccc cgacagtggc agagctgaga aagagcgtat
    14101 tcagcgttcc gatgatggga atattgcgat ctttttcggt caatgctttt aacgccatga
    14161 tttcgccagg gagactactg aactcaggat gtctgctggc accagaaagc cattcaagca
    14221 ggtaatgccg taatccggtt cactggttat attgtggttt tcctgtgccg ttttcagggc
    14281 attcagtgca caccagagca actggcgcac gggatgttct gtcgtggtca tgcgaatgtc
    14341 ctactgtgag atgaaaagaa gaccggtatc cgttctctcc gccacacgtg atggcacggg
    14401 ccggcctttg ataaaacgaa tcaatactct ggggtgaagc cttttatttc tttcgccatt
    14461 aagaaataca cggttccatc aaaagacagg gggagtccat ggtggggatg gcttcgtgca
    14521 ggcttcactc cggaatattc tcttgaattg aaaaaagccc ggcccgaagg ccgggaaata
    14581 ctggctatgg gatgtttaca aggcgctcca taaagccgtg atgttcggca tttgatacca
    14641 gttccaggag cagggatatc ctcgactcag gctggagagt attttccatt ctgaggagat
    14701 actgatattg tgcttttgtc agccatatgc tggaagccgc attcagctct tccctggctc
    14761 ccggatttag cacagggtcc gggtaaatct tcattaatcc atctacaata tctttattga
    14821 cgggttttat ttcaccgcca aacttcacga aatcaatgaa ggcgctatgg cgacgtaatg
    14881 ccgatactgg ttgaatgtca aaattagcca gaataaaaaa gtaccctgga aaaatagcga
    14941 atattctttc acggtaacag cacctggagt ctgcccgtct tatctttctt gttattaatg
    15001 gagtccatgg tacgacgttt tgctcattta gccatgagaa taaagattct ctgtttttgc
    15061 cggctgtatt atattgcgca agataccagt tccggttatt caggtgttct atattcacga
    15121 agttcaacgc tccgcgcaaa accgatccat gatggacgat tttgcgatgt caggcgaata
    15181 tgctagatca tatgtctgaa gcagtgtcca gcccatatct ggagaaactg cagttctgac
    15241 aggcatataa ccagcagact ctagtacgtt ctgtatcggt gatttgggcg aactcacaga
    15301 taaagaacgg ctaagcacta atatcagcaa tacagtggca cagtattgtg atacgcgaaa
    15361 taaaacatca gcacgtcagt tcagagtaag gtcgccatat atcgggcatg agccagatac
    15421 aaataattgg gacaccgtct gaagtgattt gctttattaa agtaacttgt gcttggtgtt
    15481 tatgtttatc aatttgggag cgcctgtaaa gtgcaaaatg caatttccat gaaagtgtcc
    15541 cctttaatgg ggacacctgt tcacattttt tcgttttctt tagctgtatt gctaatcatg
    15601 aaaacgaacg ttcgcatcag cacagcagag cgaagtgcat catatccttc cagatttaag
    15661 ttcttcaggc ttcgcttcag gtaggttcgt accgtttcgg gagaaagccg acagttctga
    15721 gcaatggcgt cgtaaggaac acccattgcg taaaaaacgc agactttaag ctggtttgtt
    15781 tgatagctcg ggaaacatgc cttccaacag agtgatggcc gggagtttct gtatcatttg
    15841 aggtgacata gcaaaggtct ccgatgacaa actaaaaacg ctatcaccga gttcctacgc
    15901 tcattggtga tagcccagac gggggtagga ataccggcgt catcggaaac cggccaggct
    15961 aatgcctgcc ccgcctgagc taccatagat gacgacaaga aatacaagtt cctgtcttta
    16021 tggtatggca aaaaacgacg ccaaatgaag aaattaactt ctgcgccgac gatgactacg
    16081 cgggttccta cgcccgatca cagttttgcc tgtgaccagt aaagggtact tagtataacg
    16141 taccgagtca actaaccgct taaaaaacat acaaaaaagc gtcaaaactt aacgctccgc
    16201 gccgaagcga tccaggcatg atgcttctgc tttgtggcgt tcgaacatgc tggtccatat
    16261 gcacgaaacg gtgtctgacc ctcgtccaga caatccgcag ttctgacgaa catatgaaca
    16321 aatatgctct tgtagaggct gttgttacac aattggcggg ttgtgtaaca aatggtttta
    16381 ttatctaaga atcattctat ttacgatcga atgatctaca aaatgatttt ttaaaggttt
    16441 tttagatcgt ttaattgtct taaaacagaa tattattctg ttaggttgat cggttgtaag
    16501 ataaatgatc gtgagtagaa caaaagtcga tcttttaatc caaaagaagg atctaaagca
    16561 gaaaaatctg cttttaaaaa tagattttat ttttttgacg caggtcaaga ttaactcatt
    16621 agaggtatcg gtgaggaggc actggaagag aagagatcgt tgtaatgctt ttcaaattaa
    16681 cgtaaagcgg gtatatttcg gctgttatta gctgtgcaga gggtggcact ctgtggagca
    16741 aagcggcgaa agccggacgg tagaatgcac cataaggcat tcaggagaga tggcatgtac
    16801 gggcagtaag tcagaagact gaagatgctc cggaagccat aaaaggaaaa cccccactat
    16861 ctttcttacg aacttggcgg aacgacgaaa gatagtgggg gcctcacaga atacgggtaa
    16921 agtataatga aaccgtacca gagattcaac cctgtgcagt gtataaatac acggcacaat
    16981 cgctccgcca taagcgacag cttgtggcag gtctgaagaa tactccatat aacgcagtac
    17041 actggagtca gttagcaccc gaagagcaga tccgtttctg ggaagactat gaagcgggaa
    17101 gggcgaccac tttcctggtt gaaccggaaa ggaagcgcac gaagcgtcgt cgcggtgagc
    17161 actccaccaa acccaaatgc gaaaatccgt cctggtatcg tcctgagcgc tataaggcgc
    17221 tgagcgggca gctcgggcac gcctacaacc gttctggtga aaaaggaccc ggtgaccggc
    17281 gagcagagcc tgcgcatgca catgtctctg catccttttt acgtgcagaa acgaacgtat
    17341 gccggtcgca atatgctttc cgtccggaaa aacaacgcgc tcctcgatgc catctggccg
    17401 gttctggtca gcttcagtga tgcgggcaca cataccgtag gcatgagtgt ttcccgtctg
    17461 gccagagaaa tcagcccgaa agacagcaag gggaaggtta ttccggaact ggaagtgacg
    17521 gtctcccgcc tttcccgttt gctggcgaac aggtacgttt tggtgtgctg ggtgtttcag
    17581 aggaaaccct gtgggaccgt gaaacccgcc agcgtcttgc cacgtttacg tctggataac
    17641 accggcaggc tggcagatgc tgggcgtcga catggtaaaa cttcacgaac agcagcagaa
    17701 acgactgccg tgaaagtgaa atccgccagc cagctcattc gggaaggtgt tctgccgtga
    17761 ggatgaagat atctccgtac atgcggccag aaaacgctgg tatcctgcca gcgcagccaa
    17821 ggatgcactg aaacaccgtc gtgaaaaagc ggcagccagt aagcgcgcgc cagacgcctg
    17881 aagaaactgc ctgccgacca gcagattcat gagatggcag agtatctcag gaagcgtctg
    17941 ccgccggatg aagcctattt tttgttccga tgaccatctg aagcgactgg ccatcaggga
    18001 gttggtcagc ttgaactgac gctggctgcg ccaccgccgc actagacagc ac
//
