LOCUS       NZ_CP011335             7893 bp    DNA     circular CON 01-MAY-2024
DEFINITION  Escherichia coli O104:H4 str. C227-11 isolate 368 shch plasmid
            unnamed, complete sequence.
ACCESSION   NZ_CP011335
VERSION     NZ_CP011335.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03856947
            Sequence Read Archive: SRR1980037, SRR1980038
            Assembly: GCF_000986765.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O104:H4 str. C227-11
  ORGANISM  Escherichia coli O104:H4 str. C227-11
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7893)
  AUTHORS   Beaulaurier,J.A., Zhang,X.-S., Zhu,S., Sebra,R., Rosenbluh,C.,
            Deikus,G., Shen,N., Munera,D., Waldor,M.K., Chess,A., Blaser,M.J.,
            Schadt,E. and Fang,G.
  TITLE     Single molecule-level detection and long read-based phasing of
            epigenetic variations in bacterial methylomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7893)
  AUTHORS   Beaulaurier,J.A. and Fang,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2015) Genetics and Genomic Sciences, Icahn School
            of Medicine at Mount Sinai, 1425 Madison Ave, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000986765.1-RS_2024_05_01
            Annotation Date                   :: 05/01/2024 02:09:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,400
            CDSs (total)                      :: 5,274
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 316
            CDSs (without protein)            :: 316
            Pseudo Genes (ambiguous residues) :: 0 of 316
            Pseudo Genes (frameshifted)       :: 136 of 316
            Pseudo Genes (incomplete)         :: 195 of 316
            Pseudo Genes (internal stop)      :: 60 of 316
            Pseudo Genes (multiple problems)  :: 68 of 316
            Pseudo Genes (short protein)      :: 2 of 316
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP011335.1.
            Source DNA is available from Dr. Matthew K. Waldor at Havard
            Medical School (mwaldor@research.bwh.harvard.edu).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7893
                     /organism="Escherichia coli O104:H4 str. C227-11"
                     /mol_type="genomic DNA"
                     /strain="C227-11"
                     /isolate="368 shch"
                     /isolation_source="stool sample from a 64-year-old woman
                     from Hamburg who presented with bloody diarrhea and did not
                     develop hemolytic uremic syndrome (HUS)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1048254"
                     /plasmid="unnamed"
                     /geo_loc_name="Denmark"
                     /collection_date="2011-05-18"
                     /note="also called BCG-1 (Russian)"
     gene            complement(49..483)
                     /locus_tag="AAF13_RS27310"
                     /old_locus_tag="AAF13_27315"
     CDS             complement(49..483)
                     /locus_tag="AAF13_RS27310"
                     /old_locus_tag="AAF13_27315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="WP_000274412.1"
                     /translation="MYGTCETLCRALAAKYPGDMPLMLVIWSPEEIQALADGMDISLSD
                     HEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVSENRQVTVPAELLASLIQTAEQAL
                     WKREWAARDHGLAVPECVTRRQAVINQARTLLKNNRHEND"
                     /name="DUF1380 family protein"
     gene            complement(497..718)
                     /locus_tag="AAF13_RS27315"
                     /old_locus_tag="AAF13_27320"
     CDS             complement(497..718)
                     /locus_tag="AAF13_RS27315"
                     /old_locus_tag="AAF13_27320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001104879.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPERL
                     VRQTLFRANRLLMEAYTEILELDACFKD"
                     /name="hypothetical protein"
     gene            complement(719..1402)
                     /locus_tag="AAF13_RS27320"
                     /old_locus_tag="AAF13_27325"
     CDS             complement(719..1402)
                     /locus_tag="AAF13_RS27320"
                     /old_locus_tag="AAF13_27325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="WP_000086153.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAGD
                     KTDEWLQPACNEMYRVLKKDALMVSFYGWHRIDRFMAAWKRAGFSVAGHLVFTKNYTSK
                     SAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESFTHP
                     NAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAANDDWF
                     MPEAA"
                     /name="DNA methylase"
     gene            complement(1479..1790)
                     /locus_tag="AAF13_RS30985"
                     /old_locus_tag="AAF13_27330"
     CDS             complement(1479..1790)
                     /locus_tag="AAF13_RS30985"
                     /old_locus_tag="AAF13_27330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001310011.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRVR
                     WARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGIALPSERRRLRYSTVGLTRYRTR"
                     /name="hypothetical protein"
     gene            complement(1787..2689)
                     /locus_tag="AAF13_RS27330"
                     /old_locus_tag="AAF13_27335"
     CDS             complement(1787..2689)
                     /locus_tag="AAF13_RS27330"
                     /old_locus_tag="AAF13_27335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="WP_023383827.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLLQ
                     TTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSGTG
                     QRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDLLTV
                     LPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDFDTPW
                     CQPESDVVAELSRLFGCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSPTDDDE
                     LPEVTGPAWIVDNVAHYGG"
                     /name="DUF1281 domain-containing protein"
     gene            3108..3356
                     /locus_tag="AAF13_RS27335"
                     /old_locus_tag="AAF13_27340"
     CDS             3108..3356
                     /locus_tag="AAF13_RS27335"
                     /old_locus_tag="AAF13_27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="WP_000618110.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRIDQ
                     GSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
                     /name="DinI-like family protein"
     gene            3353..3790
                     /gene="umuD"
                     /locus_tag="AAF13_RS27340"
                     /old_locus_tag="AAF13_27345"
     CDS             3353..3790
                     /gene="umuD"
                     /locus_tag="AAF13_RS27340"
                     /old_locus_tag="AAF13_27345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="WP_000109071.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDLN
                     SYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKRLL
                     LRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
                     /name="umuD"
     gene            3790..5061
                     /locus_tag="AAF13_RS27345"
                     /old_locus_tag="AAF13_27350"
     CDS             3790..5061
                     /locus_tag="AAF13_RS27345"
                     /old_locus_tag="AAF13_27350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="WP_000457515.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEAK
                     ALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDEMF
                     IDLRGINHCISPEIFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFSGVV
                     ALTAENRNRTLKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKNFSVI
                     LERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAAEKLRG
                     ERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHVWREGYR
                     YMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFAGRGTAPE
                     WQMKREMLSPAYTTRWTDLPVAQL"
                     /name="Y-family DNA polymerase"
     gene            5265..5891
                     /locus_tag="AAF13_RS27350"
                     /old_locus_tag="AAF13_27355"
     CDS             5265..5891
                     /locus_tag="AAF13_RS27350"
                     /old_locus_tag="AAF13_27355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="WP_000587689.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVLT
                     WYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVFIS
                     PLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANEAAEVLKDYPE
                     MHLANSRVCDRKAHRDAWAESMTIFETQNDKAQQEIEALVKEVIL"
                     /name="ParA family plasmid-partitioning AAA ATPase"
     gene            5888..6190
                     /locus_tag="AAF13_RS32435"
                     /old_locus_tag="AAF13_27360"
     CDS             5888..6190
                     /locus_tag="AAF13_RS32435"
                     /old_locus_tag="AAF13_27360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001245884.1"
                     /translation="MRNRANTSPRDEAAFINGGTAGLDNTKKSSDKSSVVSKAKPVSIS
                     FFEENLKDIDALITAEMVSGKGRVNRSDVVRAAVEALKTLSKTEVSRLIAEVKQR"
                     /name="hypothetical protein"
     gene            complement(6459..7320)
                     /locus_tag="AAF13_RS27360"
                     /old_locus_tag="AAF13_27365"
                     /pseudo=""
     CDS             complement(6459..7320)
                     /locus_tag="AAF13_RS27360"
                     /old_locus_tag="AAF13_27365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            complement(7503..>7893)
                     /locus_tag="AAF13_RS27795"
                     /old_locus_tag="AAF13_27310"
                     /pseudo=""
     CDS             complement(7503..>7893)
                     /locus_tag="AAF13_RS27795"
                     /old_locus_tag="AAF13_27310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     ARG             complement(6460..6941)
                     /name="TEM-81"
                     /gene="TEM-81"
                     /gene_name="TEM beta-lactamase"
                     /tool="rgi"
                     /drug_class="monobactam; cephalosporin; penam; penem"
                     /identity="100.0"
                     /coverage="55.94"
     ARG             complement(7109..7320)
                     /name="TEM-149"
                     /gene="TEM-149"
                     /gene_name="TEM beta-lactamase"
                     /tool="rgi"
                     /drug_class="monobactam; cephalosporin; penam; penem"
                     /identity="100.0"
                     /coverage="24.48"
ORIGIN
        1 catgattaat cgttctccct gaatcgccgc ccggaggcgg cgataacatc agtcgttttc
       61 gtgtctgttg tttttcagca gggtgcgggc ctgattaatc accgcctgac ggcgggtgac
      121 gcattccggg acggcgaggc catgatcccg ggcggcccat tcacgtttcc acaatgcctg
      181 ttcagcggtc tgaatcaggg acgccagcag ttcagcaggg acggtcacct ggcggttttc
      241 gctcacgtta ttgatgatct ccatcgccac gccggaagaa ataccggatt cagtccgctg
      301 gtcttccggg atgtcctcca ggcgcgccag gacggttctg atttcatgat cggacaggga
      361 aatatccatt ccgtcagcga gggcctgaat ctcttccggg gaccagataa ccagcatcag
      421 cggcatgtct cccggatact ttgctgccag cgcccggcag agcgtttcgc atgttccgta
      481 cattctcatc tccttctcag tctttaaagc acgcatccag ttccagaatt tcggtatatg
      541 cctccatcag gaggcggttt gcgcgaaaca acgtctgacg caccagacgt tccggcaggg
      601 tgtcggcatt aaagctgtac cgctgctcca tctcattcat ggttgtacgc aggtcgcaca
      661 tggcattggc cacctccgcc acttcggcgc gcagtttttc gtgtcctgca tagttcattt
      721 acgcagcctc cggcataaac cagtcatcat tcgcggcccc ctgctgcatg gcccgttgca
      781 cggcggcaag gcgttgctgc ccggcacggt gatactgctc aagcagctcg ataccgatat
      841 accggcgtcc ggactggagg gcggcgacgc aggttgagcc gctgcctgca aacgggtcca
      901 gcacaattgc gttcgggtgt gtgaagctct caatcagcgg ttgcaggctg gtaacgggct
      961 tttccgtcgg gtgatgacga ttgcctgaat atttccagcc cagcacatcc ggcagagggt
     1021 tacggggccg ggccggactg ccctttgcca ggatgtaagc gcattcgtgg cggtacgcga
     1081 catacgcaga tttggatgtg tagtttttgg tgaacacgag gtgccctgcc acgctgaatc
     1141 cggcgcgttt ccaggccgcc ataaagcggt caatacggtg ccagccgtag aagctgacca
     1201 tcagcgcgtc ttttttgagt acgcggtaca tttcattaca ggccggttgc agccattcat
     1261 cggttttatc gccagcgatg gtgcgcccgg aacggtcacg aaaaccgacg aggtacggcg
     1321 ggtcagtcag tatgaaatca atggcgttat caggaatacg ggccataaca tcgatgcagt
     1381 taccgaggac aaaacgggac ataacaacgc tccttctctt acagaggaca ccgggagggc
     1441 acccctgtgg tgcccgttcc ccgctgcctg tgggctgttc accgcgtccg gtaacgggtc
     1501 aggccgaccg tggaataccg cagccgtcgt ctttctgacg gcaaggctat gccgcgaaag
     1561 cggcttgaag ggcgttcatc agaaaagggc gacaggctgg cgtacactgt caccgacgcc
     1621 agccgggagc ggtaaaagcg cccgtcccgt gcccaccgga cgcgggacac tgaccacagg
     1681 cagcgtttat gccgcgccgc gtcagccggc gcagtaaaaa gaggagcacg gcgacatcct
     1741 ttaccggaaa agaaacgggc ggttgcccgc ccgtctccgt catacttcat ccgccataat
     1801 gcgccacatt gtcgactatc cacgcaggcc cggtgacttc cggcagctca tcgtcatctg
     1861 tcggggaaga ccattccagt tccccccaca gcacatcaac aagctctccg cgctcataca
     1921 actgccagcc acagaaatca cagccctgtt cggcgtacca gtgctccagc gtgcagccga
     1981 aaaggcggct taattctgca acaacgtcgc tttccggctg acaccacggc gtgtcgaaat
     2041 ctacctgaat gaagttttcc ccctgactgc tgatattcag gtcataccca caaggccact
     2101 tcacgccata ccgttctgta taccagtgat atgcagaagg aacaccgttc agaacaccgc
     2161 cgttaaagcc attcacttca atatccaggc gggtcggcaa aaccgtcagc aggtcaaacg
     2221 gcatggtttt ttccggcagt acgttgtcac acagacggtt ccaccacacc gatacatcct
     2281 cgttactcca gaagccacac cagtcacctc tttttgcggt gaaaagtgcg gtgatggtat
     2341 ccctgacatc gtccggtaat ccttcccagc gacgctgtcc agtaccgctc tgcagccaga
     2401 gttcatgcag catcaggcag ttttgttcat ccagtagcac accgttctgc aggtgtgtca
     2461 gccagcgtgt gaacgcgata ttctccggcg atacaacacc acgtccggca gccgtcagtc
     2521 cggggcacgg ttcaaactgc acatcttcag tggtctgcag aagtccggca cttccggcca
     2581 gaaacagctg aataccttca tttgtggcgc ggcgataaaa cggtgtgact gcgccgctgg
     2641 ccagtcgttt aatctcagcg atctgtgccg gttcaccaga aaaatacata cgattcgagc
     2701 accagtttgg catgggaatt ctcctctcac atgcggcctc catcaggccg tgccgcacct
     2761 gtaaccgggc gaccgcatac gaatgcaggt gcggccccga agccgcagcg cgtggaggag
     2821 gaacgacgaa acgtgaccgg gtgaggaggc cggcgacagc gacacacttg catcggatgc
     2881 agcccggtta acgtgccggc acggcctggg taaccaggta ttttattcac agaaccgtgc
     2941 gcaaaatgct gtggataagc tggacacagc agcccacagc aggcatacag ccacacactc
     3001 agagaattga gcgtttttta gtcacaacaa tgacatttga ctgtgtgcgg attcagttat
     3061 tagaatactg tataaacata cagcataaag aaaagggaga tgagaacatg atccgcattg
     3121 aaattctttt cgaccgccag agcacaaaaa acctcaaatc aggcacactt caggcgctac
     3181 agaatgaaat cgaacaacgt ctgaaaccac actacccgga aatctggctg cgtatagacc
     3241 agggcagcgc cccgtctgta tctgtcacag gagcccgcaa cgacaaggat aaagaacgta
     3301 tcctgtctct gctggaagaa atctggcagg acgacagctg gctgcctgca gcatgagtac
     3361 cgtttatcac cgtccggctg acccttcagg cgatgacagt tacgtgcgac cgttgtttgc
     3421 cgatcgttgc caggccggtt ttccttcgcc tgccactgat tatgctgagc aggaactgga
     3481 tctgaacagc tattgcatca gcagacctgc agccaccttc tttctgcgcg ccagcggtga
     3541 atcgatgaac caggctggcg tgcagaatgg tgatctgctg gtagtggaca gggccgaaaa
     3601 accacaacac ggggacatcg ttatcgctga gatcgacggt gagttcaccg tcaaacgact
     3661 gctgttgcgc ccacgcccgg cactggagcc ggtttcagac agcccggagt tccgcacact
     3721 gtatccggaa aacatctgta tttttggtgt tgtcactcac gtgatacaca ggacgcggga
     3781 gttacgctga tgtttgcact ggctgatatc aacagtttct acgcctcatg tgaaaaagtt
     3841 ttccgcccgg accttcgcaa cgaaccggtc atcgtactca gcaataacga tggctgtgtg
     3901 atcgcgcgca gcccggaggc aaaagccctt ggcatcagaa tggggcagcc ctggtttcag
     3961 gtgagacaaa tgcgcctgga gaagaaaata catgtatttt ccagcaatta tgcgctgtac
     4021 cacagcatga gccaacgggt tatggctgtt ctggagtcgc tttctcccgc agttgagccc
     4081 tactcaattg atgaaatgtt cattgatttg cgggggataa atcattgcat ctctccggag
     4141 atttttggtc atcagctcag ggaacaggta aagagctgga caggactcac catgggggtg
     4201 ggcattgcgc ctacaaaaac gctggctaaa agtgcccagt gggcaacaaa gcaatggcca
     4261 cagttttccg gtgtggtcgc gctgacggca gaaaaccgta atcggacctt gaagctactg
     4321 gggctgcagc cagttggtga ggtctgggga gtaggacgca gactgacgga aaagctgaat
     4381 gcgctgggta ttaacacagc actgcagctg gcgcaggcta acacggcatt catccggaaa
     4441 aacttcagcg tcattcttga gcgtacggta cgcgaactca acggcgagtc ctgcatatcc
     4501 ctggaagaag caccaccggc aaaacagcag attgtctgta gtcgcagttt tggtgaacga
     4561 atcacagaca aagatgccat gcaccaggct gttgttcagt atgcagagcg ggccgcagag
     4621 aaactacgtg gggagcgtca gtattgccgg caggtgacga catttgtacg gacatccccc
     4681 tttgcagtaa aagaaccctg ttacagcaat gccgctgtgg aaaagcttcc attgcccaca
     4741 caggacagcc gggacattat tgccgccgca tgcagagcct taaaccatgt ctggcgtgaa
     4801 gggtaccgct atatgaaggc aggagtcatg ctggctgatt tcacaccatc gggtatagcg
     4861 cagccgggat tatttgatga aatccagccc cgtaaaaaca gtgaaaagtt aatgaaaaca
     4921 ctcgatgaac tgaaccagtc gggaaaaggg aaagtgtggt ttgcggggcg aggaaccgcc
     4981 cctgaatggc aaatgaaacg ggaaatgctt tctcctgcgt ataccaccag atggacagat
     5041 ttgccagtag cccagttatg aatatgtaat ccacccataa tattgttatc ttgttatctt
     5101 gttatcttgt tatcttgtta tcttgttgtc ttgttatctt aattaatcta taaatcaaat
     5161 acttaacaac ttactaagtt gttaacataa caaaaataat tgcatggttc gcgttcagat
     5221 ggcaatatta agttgttaac ttaacagctt aatatgaggc tataatgata atagtattag
     5281 gtagtcaaaa gggtggggta ggtaaaagta cactggcggt atctatagct gcgtatctga
     5341 tgtcactagg caatagagtg attattgtcg acgcagatga ccagaaatca gtattaacct
     5401 ggtacaacaa tcgccctgaa gatctccctc atattccggt tacaggcgcg acaggtaaca
     5461 tcaaagccat gttaaaggaa cacgaaaaat cctatgattt tgttattgca gactgtgctg
     5521 gccgtgatag cgcagaaatg cgtagtggat tgatggccgc agatgtattc atttctcctt
     5581 tgcgcccatc tcagatggac ctggatgttg tgcctcatac atgctccgtt tttacagcag
     5641 caaaagattt taatgaggat gttcggggat atctcgtttt aaacatgacg cccacaaata
     5701 tgtttgttaa tgaagccaat gaggcagctg aagtacttaa ggattaccct gaaatgcatc
     5761 tggcaaactc ccgggtatgt gatcgtaaag cacatcgaga tgcttgggcc gaatcaatga
     5821 caatttttga aacgcaaaat gataaagccc agcaagaaat cgaagcgtta gtgaaagagg
     5881 ttattctgtg agaaatagag caaacacttc acctcgtgac gaggcagcat ttattaacgg
     5941 tggcacggct ggccttgata ataccaaaaa gtcatcagat aaatcatcgg ttgtaagcaa
     6001 agcaaaacca gtgagtattt ctttttttga agaaaaccta aaagacattg atgcccttat
     6061 aacggctgag atggtttcag gtaaaggcag agtgaatcgt tctgatgttg tcagggcggc
     6121 ggtagaagca cttaaaacac tatcaaaaac agaggtatca cgattgatag cagaagtaaa
     6181 acaaagataa caccttgaga taacaagata acaagataac aagataacac cttgttatct
     6241 tgttatcttg ttatcttgtc atcttgttat cttgtcatct tgttatcttg tcatcttgtt
     6301 atcttgttat cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6361 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6421 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6481 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6541 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6601 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6661 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6721 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
     6781 atcgtggtat cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6841 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6901 atcgttgtca gaagtaagtt ggcagcagtg ttatcactca tggttatggc agcactgcat
     6961 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7021 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
     7081 gataataccg caccacatag cagaacttta aaaagtgctc atcattggaa aacgttcttc
     7141 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7201 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7261 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cgaaaatgtt gaatactcat
     7321 actcttcctt tttcaatatt attgaagcat ttaccagggt tattgtctca tgagcggata
     7381 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7441 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     7501 tatcacgagg ccctttcgtc ttcaagaatt ttataaaccg tggagcgggc aatactgagc
     7561 tgatgagcaa tttccgttgc accagtgccc ttctgatgaa gcgtcagcac gacgttcctg
     7621 tccacggtac gcctgcggcc aaatttgatt cctttcagct ttgcttcctg tcggccctca
     7681 ttcgtgcgtt ctaggatcct ccggcgttca gcctgtgcca cagccgacag gatggtgacc
     7741 accatttgcc ccatatcacc gtcggtactg atcccgtcat caatgaaccg ggactgccac
     7801 gccctgagcg tcaaattcct ttatcagttg gatcatatcg gcagtgtcgc ggccaagacg
     7861 gtcgagcttc ttaaccagaa tgacatcacc ttc
//
