LOCUS       NZ_CP011285            13141 bp    DNA     circular CON 28-JAN-2024
DEFINITION  Enterococcus faecium strain E39 plasmid p5, complete sequence.
ACCESSION   NZ_CP011285
VERSION     NZ_CP011285.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03486724
            Assembly: GCF_001635875.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13141)
  AUTHORS   Huang,W., Wang,G., Zhuge,J., Ojaimi,C., Dimitrova,N. and Fallon,J.T.
  TITLE     Enterococcus faecium E39, a daptomycin-resistant strain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13141)
  AUTHORS   Huang,W., Wang,G., Zhuge,J., Ojaimi,C., Dimitrova,N. and Fallon,J.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-2015) Pathology, New York Medical College, 15 Dana
            Road, Valhalla, NY 10595, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. 3.5.0; HGAP Assembly v. 3
            Genome Coverage                   :: 460
            Sequencing Technology             :: Illumina; Pacific Biosciences
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/28/2024 08:32:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,068
            CDSs (total)                      :: 2,976
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 43 of 118
            Pseudo Genes (incomplete)         :: 64 of 118
            Pseudo Genes (internal stop)      :: 32 of 118
            Pseudo Genes (multiple problems)  :: 17 of 118
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP011285.1.
            Bacteria available from Guiqing Wang (WangG@WCMC.com).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="E39"
                     /isolation_source="blood of a hospitalized patient"
                     /db_xref="taxon:1352"
                     /plasmid="p5"
                     /geo_loc_name="USA: New York, NY"
                     /collection_date="2009-05-28"
     gene            1..933
                     /locus_tag="XM37_RS14840"
                     /old_locus_tag="XM37_15150"
     CDS             1..933
                     /locus_tag="XM37_RS14840"
                     /old_locus_tag="XM37_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002313300.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            950..1420
                     /locus_tag="XM37_RS14845"
                     /old_locus_tag="XM37_15155"
     CDS             950..1420
                     /locus_tag="XM37_RS14845"
                     /old_locus_tag="XM37_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002302544.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTLV
                     VSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            1504..1821
                     /locus_tag="XM37_RS14850"
                     /old_locus_tag="XM37_15160"
     CDS             1504..1821
                     /locus_tag="XM37_RS14850"
                     /old_locus_tag="XM37_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_002302541.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKIMVKNYGKKSYKNTNTIISFCNFFKFSFHLNSVRILL
                     F"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2111..3292
                     /locus_tag="XM37_RS14855"
                     /old_locus_tag="XM37_15165"
     CDS             2111..3292
                     /locus_tag="XM37_RS14855"
                     /old_locus_tag="XM37_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /GO_process="GO:0051301 - cell division [Evidence IEA];
                     GO:0009252 - peptidoglycan biosynthetic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="WP_002302539.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKIT
                     KELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVLTG
                     AKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIATLPI
                     GILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISDTGRD
                     FLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNIHVPVR
                     ESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVAMIFFHV
                     FENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQK"
                     /name="FtsW/RodA/SpoVE family cell cycle protein"
     gene            complement(3521..4669)
                     /gene="tnpB"
                     /locus_tag="XM37_RS14860"
                     /old_locus_tag="XM37_15170"
     CDS             complement(3521..4669)
                     /gene="tnpB"
                     /locus_tag="XM37_RS14860"
                     /old_locus_tag="XM37_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(4686..5090)
                     /gene="tnpA"
                     /locus_tag="XM37_RS14865"
                     /old_locus_tag="XM37_15175"
     CDS             complement(4686..5090)
                     /gene="tnpA"
                     /locus_tag="XM37_RS14865"
                     /old_locus_tag="XM37_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            complement(5627..6088)
                     /locus_tag="XM37_RS14870"
                     /old_locus_tag="XM37_15180"
     CDS             complement(5627..6088)
                     /locus_tag="XM37_RS14870"
                     /old_locus_tag="XM37_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            6221..6595
                     /locus_tag="XM37_RS14875"
                     /old_locus_tag="XM37_15185"
     CDS             6221..6595
                     /locus_tag="XM37_RS14875"
                     /old_locus_tag="XM37_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            6649..8775
                     /locus_tag="XM37_RS14880"
                     /old_locus_tag="XM37_15190"
     CDS             6649..8775
                     /locus_tag="XM37_RS14880"
                     /old_locus_tag="XM37_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323538.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            8772..9392
                     /locus_tag="XM37_RS14885"
                     /old_locus_tag="XM37_15195"
     CDS             8772..9392
                     /locus_tag="XM37_RS14885"
                     /old_locus_tag="XM37_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            9506..9643
                     /locus_tag="XM37_RS16620"
     CDS             9506..9643
                     /locus_tag="XM37_RS16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            10085..10465
                     /gene="mobC"
                     /locus_tag="XM37_RS14890"
                     /old_locus_tag="XM37_15200"
     CDS             10085..10465
                     /gene="mobC"
                     /locus_tag="XM37_RS14890"
                     /old_locus_tag="XM37_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            10447..11361
                     /locus_tag="XM37_RS14895"
                     /old_locus_tag="XM37_15205"
     CDS             10447..11361
                     /locus_tag="XM37_RS14895"
                     /old_locus_tag="XM37_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002305249.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            11321..11599
                     /locus_tag="XM37_RS16925"
     CDS             11321..11599
                     /locus_tag="XM37_RS16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            11601..12290
                     /locus_tag="XM37_RS14900"
                     /old_locus_tag="XM37_15210"
     CDS             11601..12290
                     /locus_tag="XM37_RS14900"
                     /old_locus_tag="XM37_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347611.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKVEEA
                     CSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     BGC             1221..11595
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..933
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          10448..11358
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaatg aaatcgtaaa gtatgaacca gaattaaata ccattccttt aagaaaattt
       61 accccaatag aaatgaactt gtttttttct attgtctcta gaatgcgcga taaaggtaat
      121 caaaaaatac gcttttattt cagccaactt aaagatttaa gtgcgtataa gcagactgca
      181 ggagaagtct ttataaaaga tttggcaaga acatacgatc atttgatgga tttacgtttc
      241 ggaagaaaaa gcaatggtgg attaacaata gaacgattta caatgtttac tgattttgaa
      301 attcatggag aagtcgataa cccttatgta gatataaaag tccacgaaaa agctttgcct
      361 ttacttaata atctagaaag ctgggtacgt ttctctttaa cagaatttcg agatttaaat
      421 agcagttatg ctaaaacaat gtttagattg ctaaaacaat accgaactca aggatgggct
      481 gaattttcta aggaagattt tttagagcta ttagatattc ctaaaagcta tagacaaaca
      541 aatataaatc agtttgtact caaaccaata aaagaagaac taactccact atttaaagga
      601 ttaactattc gtaaaaaata tggtaaaggt cgaggtaaac ctgttattgg ttatcgtttt
      661 acttggaaag ctgaaatcaa ccatgctgac gatttttcaa aagggaagca agaagattta
      721 cgtataaaat tatttaatat tgaacacaat ggagaattaa cacaaaaaga aaaatggcgt
      781 gctaaagatc gaatactaaa tcttccgtta ggtactcatg aagctgattt taaaaaacaa
      841 caacaaaccg aaaaagaaga agcggaaaaa caatcaatct caaatgagct taaacaagat
      901 ttattagaaa acttacaaaa cttattcgat tgaagcataa ggagtattta tgatagataa
      961 aacgatcaaa gaactcgcgg aagaatttca tgtttcaaaa caagcaatta ggaaaaggct
     1021 atcagctgat tttagagaaa actatgtgca aactgttact aggaacggag tgaaaacctt
     1081 agttgtcagc tacaaaggat atttgctttt aaaacaacat tttttaggta gtaacaccac
     1141 tggtaaccaa gtagaaaccg ttgctagtaa cgatgaaaac tcgattattg tttttttaca
     1201 tgaacagctc caagaaaaag atactcaaat aaaacaaatg caaaagttac tcgaccagca
     1261 acaacaactc accttgcagg caaatcaaca gatcgaaaaa ctgcaagaac agttacaatt
     1321 agcttatacg gaagaacctg aagaagagga attaacttct gtcaataaag aaaacaacaa
     1381 tgcaataaca acagaaaaaa agtggtggaa attttggtaa caattaccaa ctttccataa
     1441 agaagaaatc aatttctata gactctatct ttctaagaat aaaaaatatg tttaggtgat
     1501 caaatgacta taagtaaagt aatcaaacaa aaaagaatag aaaaacaact aactcaagaa
     1561 gatttagctg aaatgctttt agtatctaaa aaaaccattt ctaattggga aaatggtcga
     1621 acaataccag atacagaaaa tttaattcag tttgctaagt tatttgattt atcactagac
     1681 aatattttgt taggagaaga aaaaattatg gtaaaaaatt atggtaaaaa atcatacaaa
     1741 aacactaata ctattatttc attttgtaat ttttttaagt ttagttttca ccttaattca
     1801 gtccggattc ttttatttta gccattcaat tgccacttgg ttattaatat tttttctaag
     1861 tttactatta ataattaata cattaataat taatacttta attattatct ttaataaaca
     1921 tagaaactta tttcttcaca gcatatttcc aattgtttta ttaatttttc tatactttat
     1981 ataaaaagat agaactattt agtaagataa aaaagtagct aattgtaatt ttcgtctaaa
     2041 actcaaaact agtaaaaata actttattgt gttattttta tttataaatt atgaaaaagg
     2101 acctttcgaa atgaaaaata atagaatcta caaaaatcag ttgaattatg ggcttttatt
     2161 gccagttttt ctgctatgta tcattggatt actttcttta tatgttgctt tatatcacga
     2221 ctcttacact aattcaaaaa tcacaaaaga attaattaaa caagtattgt ggtatagtat
     2281 tgggttcact tcagcaataa ctatttattt ttttaatcct aaaattttgt ggaaattaac
     2341 tcctatattt tacggattat gcatcataat gttattactt ctcttgaaat tctataatcc
     2401 acaattagca gtcttaactg gagcaaaaaa ttggttttat attgctggtt tttcgtttca
     2461 acctactgag ttagtcaaaa taggatacac ccttatgcta gctaaaataa ccgtcaatta
     2521 tcagcaaaaa tctattacta gtaatacgaa agatgattgg atttatttac ttaaaacttt
     2581 tattgctact cttccaatag gaatattatt attttttcag aacgatctag gaacaatgtt
     2641 agtcttcatt gttatttata tttttatttt gtttacgtct aacataaata taaaaataat
     2701 tgccccaata atcgccattg gtgttgtttt atccttaata tttttatatt tggttatatc
     2761 agatacagga agagatttct tattaaaaat aggatttcac gactatcagt ttaaaagaat
     2821 agactcatgg ttgaatccat ttttagatcc aaatggatct agctatcagt tagcaaatag
     2881 tttaattgct attggctctg gcggactttt aggtactggt tttaatgtat caaacattca
     2941 cgttcctgtt agagaatccg atatgatatt ttctgtaatt ggagaaaatt ttggctttat
     3001 aggaagttgt cttgtaatat tcctatactt ttatttaatt tatcaaatgt ataaaacatg
     3061 ttttaaaagc aataacttat ttttcaccta tatcggtata ggcattgttg caatgatttt
     3121 ttttcatgtt tttgaaaata taggtgcaag tatcggatta ctacctctaa ctggtattcc
     3181 actcccctat attagtcaag gtggttctgc cattcttagt aattttttgg ggttgggatt
     3241 tatgttgtgt tgtagtagat atactaacga aaaaaaagga acacaaaaat gaattcaaaa
     3301 taacacttta tgaatagaat gtctatttca ataaaaatta tgatacctta aagttaatca
     3361 gtgaactact caccacttag ctaaacctga cggttcttaa cgcttgaagt gggagcttct
     3421 tgggaataga gcatacttga tagcgtattt ctttactaac agcggtgtct cttatttacc
     3481 aagctatccc cgtagttcct acggttcttg atgattggat ctaagccaat cccatagaat
     3541 gtagtcttag accttcggtc agaatattga tactagcatt gatatcacga tcatgatgag
     3601 tatgacaaac aggacaagtc cattctcgaa tttcaagaga tttcttgcca tctttatgtc
     3661 cgcactctga acaaatctga ctagacggaa accatttatc tactttgatg atttttcgtc
     3721 cataccagtc agccttgtat tgtaatttag tcacaaactt tgaccaagaa acatcagaaa
     3781 tacttttagc taatttatga tttcgcaaca taccttttgt gtttaagtct tcaatacaga
     3841 taatatcgtg atttttgatc atttctgtac tcaacttatt cagaaaatca gtacgttgat
     3901 tcattacttt ttcatgcaat ctagctactt tgcgtttttg tttttgataa tttttagctt
     3961 caaatagatt ggtaccttta tttttagcca ataaagcacg cctagacaat ttgcgttgtt
     4021 cacgctttag tttcttttcc attttggacg tgaatttatt attatcaatt ttttgtccat
     4081 cagaaagaat cgcaaagtcc ataataccta agtcaatacc aattgtggaa ttagttttag
     4141 gtaattcgat aatttcttct ttacacaaca aagaaatata gtatttaccg ctagaatgac
     4201 gtgatattgt agcggatttg ataatccctt ttggttgtct atgaagctta attcttacta
     4261 atgacttcaa tttaggaact ttgatgaatt tactatcaat caaagcaact gtaccatttt
     4321 gattattcgt tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa
     4381 cggatttatc ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta
     4441 aagcaaggct atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg
     4501 gaaaagtcat ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat
     4561 caagcattag attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt
     4621 gttcctctgt tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct
     4681 actttctatt tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt
     4741 gcgccaccag ttgtcagaag acaaaaactt ttagaccaga acatttcttt ccaaagaaat
     4801 tgtttgactc ttggaaagtc acgttttatc aatcttgagc tggcactttt ataggcattg
     4861 atgaattttg tcaattctgt tttaggttga gctttgaaca taatatgaac gtggtcttta
     4921 tcatgattcc actccactaa agtaatgtga tacgattctg aaattctttc aaaagtagtt
     4981 ttggcataat cagatatttc atcatcaatc acttgtctac gatatttcgt tactaaaaca
     5041 aggtggtaat aaagaagaaa tactgaatga ttatttgtat ctaattccat gttatatcaa
     5101 ctcatttctt gtatagactg attgtaacat ggaacaaaat aaaaaggcaa tacgccttag
     5161 ttgtcggtct tcgagttacc aacggtaacc ctcataccga acggcattca tcacccacct
     5221 atagaggatg ggcgacttct gcctaattct gttaaattca aattattaca acagtcctaa
     5281 cgaagaattg tttgtaaaac aaacggataa ataataaaaa agactgctct ttcgagcagc
     5341 cagttaaaaa ataatgagtg tttattcttg aaaaagcaac gcgcttatga agcgctacca
     5401 aaaatttatc atattttatg aaaaataaca ccccagtaaa aaatgactgg taaaaattca
     5461 caaagaaacg gaaagaagca actgcagaaa atcaagcctt aaaaagagct tttgacctag
     5521 accaatgaaa aatcacaaag tgagcccctg tatcccttgg tagagaggct acttggcata
     5581 gtcgacatta tgttaactat gcaattaaat aactaactac ttcatattag cttaataaaa
     5641 taatcaaaat aactaggggg aaaaacattg tataaaaaaa tttttcattt tttaaataag
     5701 aatagtaaat ataaaaataa tttaagatat ctattactat taatccagat agagcaaacc
     5761 aagtaaatat tccatgtgta aagctaaaaa aattcatttg aatcaattca aataaaccta
     5821 atatagtaac aaatatatta aaaataatag ctgtcaatac cagtaaatta ttttttttat
     5881 tttttgaatt atcttcattt aaaatagaat ttatgttagt actataaaat tcacaaattt
     5941 tttttagtgt ctctatatct ggtgttgttt tgttattttc ccactttgaa atagtttgac
     6001 gtgtaacgtt aaattttctt gccacatatt cttgagtata cccttttttc actcttaatt
     6061 ctcttagctt agatcctaaa tcctccattt tttctcctat gtaaacgatt atttacataa
     6121 aattttatca taaaaatact aacgcgccaa aaaagttctt tattttataa aacacactaa
     6181 aataaaataa ttctagtaaa aaagatagga gaaataagtt atgaaaaaat ttttatgtct
     6241 ttctgcttta atgggtgtct tattaactag tggaggaata gctagtgcca cagaagcttt
     6301 aaatctagat gtacccgaag accataccgc catttacgga ggaggaatgg aagctacaga
     6361 agaaggattt tcttctagta aattgagata tgctgctggc ggcggggatt tcaactgtgg
     6421 ggttaatgga tttaaagttt atgcaaatta ctaccatgct agagctaaac atagtgcaac
     6481 agctaaaaat ggacgaggcg gtcaagtacg atcagttcag aaagctggag taagagctta
     6541 tgcaacatgc aatgccacac ttactggaaa tactggttgg tggaatgttt attaaaaaag
     6601 agagtttaaa ctctcttttt tatttacatt tgaaaggaaa ttcaaaatat gttaaaaaaa
     6661 ataaacgctg ttttatcact ttttgtctgc attacaatac tatttttttc tactaatgtt
     6721 ataaatggta taaagacttc tgaagcgcta tataaaaata ccgctgaaat tcatatgcaa
     6781 tataaagatg atatggactt taacagttat ataaacgctt tgtatgaaat atctacacaa
     6841 gaaaatatta atatttctca atatagtttt actgctgtta accaactaac tattcttgct
     6901 acaaacccta acgctaacaa acactggaaa ttttctaaaa aaaatatatt agcaagaaac
     6961 gattctaaag tccattatac taataacaaa aattctaagt accaattaaa acttccaaat
     7021 aattttttaa atataaatat ttatcctttt tctcatgtgg aaaatattgg attaagtgaa
     7081 atattatatg tacaaggaaa ctcaaacaaa ttaattcctg tactgaaaaa atatggaaca
     7141 cctactattt ccaatatcaa tcaatcagat acttttcaaa ttaatactaa tattgttctt
     7201 gttatctgct atcttagtat atttattgta attactacaa ttatatttgc tttttcaaaa
     7261 atgaaagaaa tcactctaaa aaaaatgtta ggctataact cttttgatac tataaaagat
     7321 tctattaaag agttaaacct cttattttgt tctttgatta taactttcat tttatctatt
     7381 tttttgagca tatattacag taacgaaaat tttttcctat catttcttct atgtagtatt
     7441 tttcatttta taattagttt tttatttatc atagttactc taagtatcta tcatttatct
     7501 agtataaaaa attatctaaa aaattcacga ccacttaagt taaatctttg tatattaaat
     7561 atctgtctat tcttaagcat gatccttctg ttaatagctt ctacaaaagt aataaatacc
     7621 cataatgaag ccgaaaataa ttctcttaaa tattgggaaa gaactactaa tttatacaaa
     7681 acaaatatta ctaatcaatt aaatagaaac aacaccgtag aagaaaataa ctacttgaaa
     7741 aaagctagta aatttgttta taaaatacag aagaattata aaacttttat tatcgcgcca
     7801 tacaattacg caactatcca agagaataat aaagaatttt ttattggaca aaaagtttat
     7861 ccaaattttg aagactatgt tagtcaacct gctggtgcag gaatatgtgt tgatttaaac
     7921 tatctaaaat attacaatcc tatttctttt gaagaatcta ctgacttaaa tttaataaac
     7981 tctgatccac taactactta cattctagta cctaaaaaat ataaagaata tgcaaaaatg
     8041 attgaaaaaa attatttaga agacatacag tttagacttg aggactctcc tcctcaagct
     8101 ccttataaaa atcctaattt gaaaaattta aaaataaaac taatatttgt taataataac
     8161 caaaagtatt ttagttttaa taccttatat ggaaaggcaa aagataacta tacaataact
     8221 gatcccatag ttagagttat taatccagaa ataacagaat cattattttg gggaaatata
     8281 ttgactagtg atggtggatt attttttgat cagaaacata cttcaaacca aaactttttt
     8341 aatcaaatta atccgtatat aaaagaattt aacctagaaa atatcataaa tttttcttat
     8401 tctgtttttc aagaaactgg tgaattatta tctcaaaaaa aagtaaataa attaaatgca
     8461 tatctacaat atcttttaat attaggatta tttataataa ttaattttca aatgattttt
     8521 gttagtttta ctataaattc aaaaaaaata gctttaaaaa aaatatttgg atattcacca
     8581 ataagtcaag ttttagattt atgtttactc ccttttacaa tagagttatt tgctactatg
     8641 tgttttcaat tgatattttc aaaaaacact tacttgtatt ttataatttt tatgatcttt
     8701 ttaataaatc taattacttc tattattatt tataaaaaaa atagttttaa atattttaag
     8761 gagaatattt tatgatatta tcagttgaga atttcaccct gtcaattaat aaaaaaaaga
     8821 tcaaacaaaa ttggaacttt tcaatatcag aaaaagattt tattatagta acaggaaaaa
     8881 gtggtagtgg aaaaactact ctgttaaata atttaagcct aatagataaa caatataccg
     8941 gtaacttatt ctattttgaa aagaaagttt ctaaacatga tatacaacgt ctacgaagaa
     9001 atcatattag ctatttattt caaaactatg ctcttcttga aaatcaaaca gtacaataca
     9061 atttcgaatt agcaaaaaag tttaacagta atatttccaa tgatcatatt tataatttat
     9121 taacagattt ttctttgcca aaagatatct taaaagaaaa aatctttctc ttaagcggtg
     9181 gagaacaaca aagaatagca ttaattagaa atatgttaaa accatttgat atattatttg
     9241 cagatgaacc tacaggaaat ttagataaag aaaactcaac ttttataatg aactttctga
     9301 aaaatttaac agaaaaagaa aaaaaagccg ttgtactagt aacacatgac ttaaatgttc
     9361 taaactatgg aactaatatt atagaattat aatttttacc cgtgatgcta gctgatttaa
     9421 aactagcacc acgggtattt attttatatt ccatctctcc tatcccaaaa tttaaataat
     9481 cacttaataa aaaggaggca cccaagtgat tttattgaaa aaagagtatg aaaaatttta
     9541 tgatagactt atttattctt tgcaattgcc aaaaaaatta aaccgtcaat tacaaaagac
     9601 ttatgaaact aaatcaactg aagaatggga gagttattgc tagatttttg gtagaagtaa
     9661 caaaaagtgg acatattatc ataatgactc gccgaaagtc tgataaaaaa ttggctaaaa
     9721 atattgctga atgtttaata aaactatcag ttttagctaa atagttagta gtttacacaa
     9781 aaaagcattt atgttttttg ttgcaaagca acaatttcat cttcggtgaa ataagcgaaa
     9841 ctttacaaaa agtggctagc atttgctagc tttttttgta ttgtggagcg tggcttttga
     9901 gggggattca agggggagct tgcgcccctt tgacaagcgg ttatttgcac taaatacgta
     9961 ggtatttggc tagcaaatat agagcttgcc aaaccctttt agtgagcttt actcattgta
    10021 cctttttagg tacaatggta ttgcgaacga tacgtccgta ggacgtactg aaagggtggg
    10081 acttatgagc gaacaaaatc aaaatttggc tagcaattct tctaaaaaat atacctatcg
    10141 atccgagcca aaacaaatca gttttcgagt gagcgaatcc gaatttgcaa agctgaagca
    10201 atcggctgaa gctttgcaaa tgagtgtgcc ggctttcgtg aaagccaagg cacaaaacgc
    10261 gcgtctggtg acaccgaaag tggcgccaga cattgcccag gcaatcgcgc gcgatttagc
    10321 caaagctgga gggaatatca atcaaattgc taagtggtgc aatacacacc aacatgacgt
    10381 tgcttctggt gacgctcaac gtttatccga aaacctaaaa atcatgcaaa aggagctaca
    10441 aaagatatgg caacaattaa ggtaagccga accacttctt gtagtcgtgc aattaattac
    10501 gcagaaccac gagcaaccat taaaacagga ataaactgtg acgtcgatta tgccaaaagt
    10561 gaaatgaaac aaattcgcat gctgtatgga aaagacgacc gtgtacaggc acacttattg
    10621 attcaatctt ttcgaccagg agaaattacc gctgaaaaag ctaatcaact aggggcagag
    10681 tatgcaaaaa aaatagcccc cgatcaccaa atagctattt acacacatac agacaaagac
    10741 catattcata atcacattgt cattaactcc gttaatctgg agactggaaa aaaatttcaa
    10801 gctcatggtc aagcatttct aaacaaatgt tatgacctaa atgacgagat ttgtcttgca
    10861 cataacttac caatcactga gcgaggaaag aaaccagaaa aacgaaccat gtctgaaata
    10921 aaattaaaag aaaaaaatga acctgtttgg aaagacgaaa ttcggattgc gatcgatcaa
    10981 acaatgaaaa acaaaaaaac cagaacctat gaccaatttt gtaattccct caaaacattg
    11041 gggattcatt gcttcaatcg cgggaaaaat ttcacttacg aattgatcca caaaaagaaa
    11101 aaagtacgat ccaacagact aggaaaggac tacgaaaagg agacgatttt acatgagctg
    11161 gacagacgag aacgaaccta taaccaacag cgaaccaacg agctacgaag agctcgagct
    11221 agcattcgcg aaactgcaga agaagtacga agagaacaac gaactcttac gcctgaacaa
    11281 ccggaaatta gaccggctcg aagaaaccat gaacaacacg ttgaaagagg aacagaccac
    11341 caacaagaat tatcgctcta aaacgttaaa agacatagaa aacgaaataa agaaacaacg
    11401 tgcttttctc gttccttata cgcaagaaag gatacaaact acgattgatt tacaagttaa
    11461 aaaactccat caagagctat tagaccagcg aaataaggaa cagaaaaagg tgcttattca
    11521 acgatcattg gattttcttg cccccttatg ttgcttagtc ctgacactgt tcgcattttg
    11581 gttagtatgg ggaggctaaa atggcatttt atttacccta tctactcatt tttgttagta
    11641 tttcaggaag tatttggctc atctataaaa tttttcaaac aagatattca ttaaaaggct
    11701 ctaaaattcg atttaagcga tttttctttc ttggctgtat attttccttg ataatcgttt
    11761 caagcggctt attcggcgtt ttagagggaa ataagcgcgt ttctagaagt attttattag
    11821 gaaatgtcac tcaaaagtat gaatcagcgc gtgataaaaa gaaaaaagag caagcattag
    11881 cacaaaaaat ggaagagttt accacttgtt atgaggaaat gaacgatatt tttgtgaatc
    11941 aagaaaaacg tttgacggat aaaaacatgg aaaaactcac tcgtctttat caaaacttgc
    12001 cagaaaaaca gcaaaaagag gttcaagaca actatgagcg aaccaaaaaa gacgtgcaat
    12061 atgtgaaaga tacgaaagtg gaagaagctt gtagtgattt atttggagat acaattcctt
    12121 tttcaactag cgaacaagaa cgaaaagaac gacaacaatc agtcacatat gaacgctatg
    12181 aaatcttgct tcaacaagca attaacatac aaaatctgac taaaaaagaa acagctttaa
    12241 attatttaaa aagcgtcaag gaatggttag atcagcaaca gcaaaattga atttgattga
    12301 aattcatgtt aaactaaaca tgcaaatgtt caaacattag atacacgaaa gccccagaga
    12361 ggtcggattc tctggggctt tttttaggtt gatgtcgcct ttactgattt tacttcttgt
    12421 ttcggttatt caaccaataa ctgaatacag agatacaaac tcctacaaga agggggcaaa
    12481 taaacagttc aaacatcaga tacacctcct tccagtcgca agactgtcgg ataggcgaca
    12541 agttattata ccctacaaaa ttaaatctgt gtggcaattt taagtatctc ttatttccgg
    12601 gttgttaggg cttgccctga ccgcctgtag ggcgctttaa attccagata aagtttttta
    12661 tactaagact gtcttaaaac ggcttagaca agctcttaga gcctcaaccc tcctttttga
    12721 acaatggttt tagcaaagga gctggaggcg actgcgcagg tataatgtga gagcgtagcg
    12781 atcacacctt tttggcgacc gtagggagcg aggttttgaa ttagcagttt agaaaagttc
    12841 gcgaacctgt ttaaagaaaa aataattgtt tttttaggtg ggaaattttg tctccctctt
    12901 ttataatctt tttttaaacc tctttttaaa cctctttaac gtcggctgtg tccttactcc
    12961 cccaaggtat acaaaatttt ataggcatga aattatctgt ttataggcat gaaattatct
    13021 gtttataggc atgaaattat ctgtttatag gcatgaaatt atctgtttta ggtattgttt
    13081 ttagacataa tatgtcctaa aatgatgtca ataaaatcat ttagactgga gtaacaacta
    13141 a
//
