LOCUS       NZ_CP011142             1783 bp    DNA     circular CON 01-JUN-2024
DEFINITION  Escherichia coli VR50 plasmid pVR50H, complete sequence.
ACCESSION   NZ_CP011142
VERSION     NZ_CP011142.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03371467
            Assembly: GCF_000968515.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli VR50
  ORGANISM  Escherichia coli VR50
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Beatson,S.A., Ben Zakour,N.L., Totsika,M., Forde,B.M., Watts,R.E.,
            Mabbett,A.N., Szubert,J.M., Sarkar,S., Phan,M.D., Peters,K.M.,
            Petty,N.K., Alikhan,N.F., Sullivan,M.J., Gawthorne,J.A.,
            Stanton-Cook,M., Nhu,N.T., Chong,T.M., Yin,W.F., Chan,K.G.,
            Hancock,V., Ussery,D.W., Ulett,G.C. and Schembri,M.A.
  TITLE     Molecular analysis of asymptomatic bacteriuria Escherichia coli
            strain VR50 reveals adaptation to the urinary tract by gene
            acquisition
  JOURNAL   Infect Immun 83 (5), 1749-1764 (2015)
   PUBMED   25667270
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Forde,B.M., Ben Zakour,N.L. and Beatson,S.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2015) School of Chemistry and Molecular
            Biosciences, University of Queensland, 76 Cooper road, Brisbane, QLD
            4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRTanalysis v. 2.0.0
            Genome Coverage                   :: 65x
            Sequencing Technology :: Pacific Biosciences SMRT sequencing
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000968515.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 04:12:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,102
            CDSs (total)                      :: 4,969
            Genes (coding)                    :: 4,733
            CDSs (with protein)               :: 4,733
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 18
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 82 of 236
            Pseudo Genes (incomplete)         :: 158 of 236
            Pseudo Genes (internal stop)      :: 43 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP011142.1.
            Source DNA is available from Prof Mark Schembri
            (m.schembri@uq.edu.au).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Escherichia coli VR50"
                     /mol_type="genomic DNA"
                     /strain="VR50"
                     /serotype="OR:K1:H-"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:941323"
                     /plasmid="pVR50H"
     gene            85..1074
                     /locus_tag="ECVR50_RS26165"
                     /old_locus_tag="ECVR50_H001"
     CDS             85..1074
                     /locus_tag="ECVR50_RS26165"
                     /old_locus_tag="ECVR50_H001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001529700.1"
                     /translation="MKNPELQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MALCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGEILNRMNAAFQRLKDRKEFRPVQGWIRTTEVTRSS
                     DGSAHPHFHTLMMVPPSMLSGDGYVKHARWVELWRECLRVSYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESE"
                     /name="protein rep"
     Typing          complement(801..992)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.409"
                     /coverage="100.0"
                     /evalue="6.18e-88"
ORIGIN
        1 ttcgaattgg tgatacggct aacacaggtg atatggcaat gataacacgt cagatgagcc
       61 atcgcccaca atcgggtggt gtgcatgaaa aatcctgaac tccagaacct cacggactat
      121 tccccctcag atgcgccctg ggacgctcac agaagcgttt ctgacgacgt gggagggatt
      181 tacctgcttg cggccgaata tgagcgctac ggggcgcgta tggccctctg tggcggttta
      241 ttgcgtttcg ggtggtctac cctcaaggaa accggagaaa cgcgtctgag gctgcgtgag
      301 gcgcatttct gccgtgtccg gcactgtcct gtctgccagt ggaggcgctc cctgatgtgg
      361 caagcccgtt tctaccagtc cctgccgcgt atcgtggcgg attaccctga tgcccgctgg
      421 atgttcctga ccctgaccgt gcgcaactgt gcgatcgggg agctgggcga gatactgaac
      481 cggatgaatg cagctttcca gcgactgaag gatcgcaagg agttccggcc tgtgcagggc
      541 tggatacgta ccacggaagt cacccgcagc agtgacggca gcgcacatcc ccacttccac
      601 accctgatga tggtgccgcc ctcaatgcta tctggtgacg gttacgtaaa acatgctcgc
      661 tgggttgaac tctggcggga gtgcctgcgg gtcagttacg acccgaatgt tgatgtccgg
      721 gcagtaaaac cccggaagcc gaaggacggc gaatcgctgg cctgcgcgac cgccgaactg
      781 gtgcgcggtg cggtggccga aaccctgaag tattccacga agccggctga catggtggcc
      841 gatcctgaat ggttcctcga actgacccgc cagacgcaca agcgccgctt tgtggcgact
      901 ggcggcgcac tcaaagacgt actgaagctc gatcaggaaa ccgacgcaga catggtgatc
      961 ggggatgata tttccgaggg cgacgatgac ggttcccgca tcgcgttcga gtggaaaact
     1021 gaggtcagaa agtaccgtcg ctcaccgacg aaagataaaa ccgaatcaga gtaacggggt
     1081 gtcggggctg cgccctgacc atgaggactt tgggcatccg tcaccttggt ggcggtgtac
     1141 caaagtcaca tcttgtccga ttggttttac tccgatgcag gatagggcta acgccgcaaa
     1201 gtgctagcac tcgattgcct agttcggaat ccgaacttag ggttttgcga tccgtaatac
     1261 ggattttttt tgacgggaca agatgtgttt ttggagtacc gccatcacga tgcgggtacc
     1321 aaaaacgatc cgtcagggct gcgccccgac accctgccaa cacctcacgc cgccagattg
     1381 aaatctgcca gcgttcgccg ttcctgaaat cgtcaaacgc taaatcgtgg ggtcccgctg
     1441 gcttgccagt gggttccgat ttagtgattg acgaccgcgc ccgtaaaaca ctgccgacag
     1501 gtggttgtga tcacgcagtg agcgcaacca cctggtaagc ggttttgggt ttttttgggg
     1561 gtgatttaac gcggttttcg ggtggcaaat ctcgcgcgcg atccttgtat ttatacttaa
     1621 gggataactc tgggattcaa attggcctcc agcccttgtc ccgcaaggct tgagggcaca
     1681 tttgctccga aatgggttta gaagtattct gtcggaagtg gtcgcgttaa aagcgacccc
     1741 cgcaacgacg ggcatcgttc gggggcgtga ccacctgtag aga
//
