LOCUS       NZ_CP011141             2089 bp    DNA     circular CON 01-JUN-2024
DEFINITION  Escherichia coli VR50 plasmid pVR50G, complete sequence.
ACCESSION   NZ_CP011141
VERSION     NZ_CP011141.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03371467
            Assembly: GCF_000968515.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli VR50
  ORGANISM  Escherichia coli VR50
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Beatson,S.A., Ben Zakour,N.L., Totsika,M., Forde,B.M., Watts,R.E.,
            Mabbett,A.N., Szubert,J.M., Sarkar,S., Phan,M.D., Peters,K.M.,
            Petty,N.K., Alikhan,N.F., Sullivan,M.J., Gawthorne,J.A.,
            Stanton-Cook,M., Nhu,N.T., Chong,T.M., Yin,W.F., Chan,K.G.,
            Hancock,V., Ussery,D.W., Ulett,G.C. and Schembri,M.A.
  TITLE     Molecular analysis of asymptomatic bacteriuria Escherichia coli
            strain VR50 reveals adaptation to the urinary tract by gene
            acquisition
  JOURNAL   Infect Immun 83 (5), 1749-1764 (2015)
   PUBMED   25667270
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Forde,B.M., Ben Zakour,N.L. and Beatson,S.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2015) School of Chemistry and Molecular
            Biosciences, University of Queensland, 76 Cooper road, Brisbane, QLD
            4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRTanalysis v. 2.0.0
            Genome Coverage                   :: 65x
            Sequencing Technology :: Pacific Biosciences SMRT sequencing
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000968515.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 04:12:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,102
            CDSs (total)                      :: 4,969
            Genes (coding)                    :: 4,733
            CDSs (with protein)               :: 4,733
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 18
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 82 of 236
            Pseudo Genes (incomplete)         :: 158 of 236
            Pseudo Genes (internal stop)      :: 43 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP011141.1.
            Source DNA is available from Prof Mark Schembri
            (m.schembri@uq.edu.au).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Escherichia coli VR50"
                     /mol_type="genomic DNA"
                     /strain="VR50"
                     /serotype="OR:K1:H-"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:941323"
                     /plasmid="pVR50G"
     gene            703..1668
                     /locus_tag="ECVR50_RS26155"
                     /old_locus_tag="ECVR50_G002"
     CDS             703..1668
                     /locus_tag="ECVR50_RS26155"
                     /old_locus_tag="ECVR50_G002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1672..1938
                     /locus_tag="ECVR50_RS26160"
                     /old_locus_tag="ECVR50_G003"
     CDS             1672..1938
                     /locus_tag="ECVR50_RS26160"
                     /old_locus_tag="ECVR50_G003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          1097..1328
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 aacactctgc gagtggtaca ttttccccgg attatcgtcc tgagcctgcc gctggctctc
       61 tttctaccgc ctcgctttgc tcgttgctca acgcctcaca gacacggatt aaaatccgca
      121 ttcgttcacc gttttttaaa gtccgttaaa agcatgatgc catctccgag agttaatctc
      181 gtcaaatgct aaatcgtggg ggtccccttt ggggttccga tttagtgatt gacgacacca
      241 ccgattaaaa aacttatgcg gggtggatgg tttcacgaag tgaggccatc cacctgtaag
      301 acagggtttt gtttttattc cctgttttgg tgatcgggtg tgtggaaaag gttggggtaa
      361 gccgttcggg ggtgcttgtt ttggggggtt aaaattgtgg ttattttttg cgcaattctc
      421 gcgcgtgatc cttgtattta tacttaaggg ataaatggcg gatatgaaat agtggtttag
      481 cccagtaatg acgaggcttt gagtgggttt tgacaggtca aagaaaatgg agcagaattg
      541 aggcgttttt aatcggcgtt ggggagtgcg tcaacactcc ccaacatttc gaatgtgtca
      601 cctcagcggc aaactctggt gacatgtact ggctcgcaat gcacaggtac gtgatgaata
      661 taccacatca aatcacagcc tgcccagatc ggagcaggct taatgtcaga agataaattc
      721 ctttcggact acagcccccg tgatgcagtt tgggataccc agcgcacgct taccgattct
      781 gtcgggggta tctaccagac tgctgctgaa ttcgagcgct atgcactccg tatggcctcc
      841 tgtagcggtt tgttacgttt tggttggtct accatcatgg aaaccggaga aacgcgccta
      901 cggcttcgta gtgcgcaatt ttgccgtgtc cgtcattgcc ctgtctgcca gtggagaaga
      961 accctcatgt ggcaagcccg tttttatcag gctctaccga aaatcgttgt ggattacccg
     1021 tcttcccgat ggttgtttct gacgttaact gtcaggaact gcgagatagg tgaacttgga
     1081 acagtcctta cagcaatgaa tgcggcgttt aagcgaatgg aaaagcgaaa ggagctatca
     1141 cctgttcagg ggtggatcag ggctacggag gtgacgcgag gtaaggatgg cagcgcacat
     1201 ccgcattttc actgtctgct gatggtgcaa ccttcttggt ttaaagggaa gaactacgtt
     1261 aagcacgaac gttgggtaga actctggcgc gattgcttgc gggtgaacta tgagccgaat
     1321 atcgatattc gggcagtaaa aactaagaca ggtgaggttg tggccaacgt tgccgagcaa
     1381 ctgcaaagcg cggttgctga aacgctgaaa tactccgtta aaccggaaga tatggcaaac
     1441 gatcctgagt ggtttcttga gctgacgcgg cagcttcaca agcgccgttt tatctcgacc
     1501 ggtggggcgc taaaaaacgt cctccagttg gatcgagaaa ccaatgagga tcttgtcatt
     1561 gccgacgatg taggggatgg cactgatgac gggaagcgga cggcgtttgt ctgggattca
     1621 ggtaaacggc gttacaaacg cgcccctgag aaggataaat cggattaacg tatgaatatt
     1681 aatattgaat acctgaatgg aaataagact attggtttat tttttttaag aagtgaagcg
     1741 gtgattcctg acaggtttaa aaaccttatt ttgcttattg atggattaag ttttggcaca
     1801 tttggttttc atccgcacga aggttttgag gatgaattaa ttttatatat tcagaaaaca
     1861 aacgagaggg taaaaactct ttttgtgaaa atagatttaa acggtatcaa gtttggattt
     1921 ttaagaacgc attcttagtt ctggaaaaga gccagcggca ggctgaggtg ataggtacga
     1981 gattgcatgc aatctctagt gctctgtcta tcctgcatta tcctcagcat tatcctcagc
     2041 cttgccaact cgacaccaat gcaggataga caatccgatg tcaaatgtt
//
