LOCUS       NZ_CP011137             6989 bp    DNA     circular CON 01-JUN-2024
DEFINITION  Escherichia coli VR50 plasmid pVR50C, complete sequence.
ACCESSION   NZ_CP011137
VERSION     NZ_CP011137.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03371467
            Assembly: GCF_000968515.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli VR50
  ORGANISM  Escherichia coli VR50
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Beatson,S.A., Ben Zakour,N.L., Totsika,M., Forde,B.M., Watts,R.E.,
            Mabbett,A.N., Szubert,J.M., Sarkar,S., Phan,M.D., Peters,K.M.,
            Petty,N.K., Alikhan,N.F., Sullivan,M.J., Gawthorne,J.A.,
            Stanton-Cook,M., Nhu,N.T., Chong,T.M., Yin,W.F., Chan,K.G.,
            Hancock,V., Ussery,D.W., Ulett,G.C. and Schembri,M.A.
  TITLE     Molecular analysis of asymptomatic bacteriuria Escherichia coli
            strain VR50 reveals adaptation to the urinary tract by gene
            acquisition
  JOURNAL   Infect Immun 83 (5), 1749-1764 (2015)
   PUBMED   25667270
REFERENCE   2  (bases 1 to 6989)
  AUTHORS   Forde,B.M., Ben Zakour,N.L. and Beatson,S.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2015) School of Chemistry and Molecular
            Biosciences, University of Queensland, 76 Cooper road, Brisbane, QLD
            4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRTanalysis v. 2.0.0
            Genome Coverage                   :: 65x
            Sequencing Technology :: Pacific Biosciences SMRT sequencing
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000968515.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 04:12:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,102
            CDSs (total)                      :: 4,969
            Genes (coding)                    :: 4,733
            CDSs (with protein)               :: 4,733
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 18
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 82 of 236
            Pseudo Genes (incomplete)         :: 158 of 236
            Pseudo Genes (internal stop)      :: 43 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP011137.1.
            Source DNA is available from Prof Mark Schembri
            (m.schembri@uq.edu.au).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Escherichia coli VR50"
                     /mol_type="genomic DNA"
                     /strain="VR50"
                     /serotype="OR:K1:H-"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:941323"
                     /plasmid="pVR50C"
     gene            complement(join(6836..6989,1..269))
                     /locus_tag="ECVR50_RS26225"
     CDS             complement(join(6836..6989,1..269))
                     /locus_tag="ECVR50_RS26225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001255518.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            391..669
                     /locus_tag="ECVR50_RS26050"
                     /old_locus_tag="ECVR50_C001"
     CDS             391..669
                     /locus_tag="ECVR50_RS26050"
                     /old_locus_tag="ECVR50_C001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(661..951)
                     /locus_tag="ECVR50_RS26055"
                     /old_locus_tag="ECVR50_C002"
     CDS             complement(661..951)
                     /locus_tag="ECVR50_RS26055"
                     /old_locus_tag="ECVR50_C002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            1192..2838
                     /gene="cka"
                     /locus_tag="ECVR50_RS30230"
                     /old_locus_tag="ECVR50_C003"
     CDS             1192..2838
                     /gene="cka"
                     /locus_tag="ECVR50_RS30230"
                     /old_locus_tag="ECVR50_C003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_162484469.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQSVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGV"
                     /name="cka"
     gene            complement(2835..3125)
                     /gene="cki"
                     /locus_tag="ECVR50_RS28475"
                     /old_locus_tag="ECVR50_C004"
     CDS             complement(2835..3125)
                     /gene="cki"
                     /locus_tag="ECVR50_RS28475"
                     /old_locus_tag="ECVR50_C004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            3155..3286
                     /locus_tag="ECVR50_RS28480"
                     /old_locus_tag="ECVR50_C005"
     CDS             3155..3286
                     /locus_tag="ECVR50_RS28480"
                     /old_locus_tag="ECVR50_C005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3481..3624)
                     /locus_tag="ECVR50_RS30985"
     CDS             complement(3481..3624)
                     /locus_tag="ECVR50_RS30985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(4292..4483)
                     /locus_tag="ECVR50_RS26065"
                     /old_locus_tag="ECVR50_C007"
     CDS             complement(4292..4483)
                     /locus_tag="ECVR50_RS26065"
                     /old_locus_tag="ECVR50_C007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4551..4874
                     /locus_tag="ECVR50_RS26070"
                     /old_locus_tag="ECVR50_C008"
     CDS             4551..4874
                     /locus_tag="ECVR50_RS26070"
                     /old_locus_tag="ECVR50_C008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5083..>5517
                     /locus_tag="ECVR50_RS30765"
                     /pseudo=""
     CDS             5083..>5517
                     /locus_tag="ECVR50_RS30765"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5552..6112
                     /locus_tag="ECVR50_RS30055"
     CDS             5552..6112
                     /locus_tag="ECVR50_RS30055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            6119..6352
                     /locus_tag="ECVR50_RS30060"
     CDS             6119..6352
                     /locus_tag="ECVR50_RS30060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_032242414.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQNMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          4120..4207
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(3553..4109)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.454"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4865..6459
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.436"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aattctttta cacgttgctg tgccgtttct gctgatactc cggctgcttt gtctttcatc
       61 cccatatccc ggaactgctg aaagaacggg agcatcatac gtatggtgtc tgctttctgg
      121 gtgtagaagt taggatcaaa tccatcatct gaagcataaa cataatgccg ggtatgtcgt
      181 tccaggctgg tctttggtgt gcatcctgta agagctattg ttgctgttat cagaggtatc
      241 agaagcagcc tggcagcggc agatctcata aacatctcct tgcgttttta atcttgatac
      301 tcagggagtt atatctccga agagtatctc ttatcatacg tcggtttact cccaatgtga
      361 tttgatggtc aatgagtcag gaataatccc gtgaaaaaat gtgtgccagt cctgcttttt
      421 agtgttcttt ctgcttcggt tgccagtcat gcggacagca atgtttttca acaaaaggcc
      481 gagaagacaa aaaacggcat tgttgcggat gttaatgcca gacagaaaat gttcagtaac
      541 agactgaaga tacaacttca gatcgatgag ttgagagcag agctggctgg caccagagat
      601 gcccaaaaaa gagaggattt gcgtaaaaaa ctggagtctc tggagcggca aaagaaatcc
      661 ttacgctgat gggggcgtat tttccccggt ttcttccata acctttttac cgttcagata
      721 tttacgggcg aggcgaatgc gtcttgcctg gtataccgcc gagtaagcct gggtgggatt
      781 cacaccatta ctgatttcac tgaccacatg acgctgatca gccagtgtca gcgtgtaaac
      841 ctgtttctgg atgaggtcac gggctttgtc atgctcatat ccggcagcaa caagggtgtc
      901 gagaagtcgt ttgtctgttt tgttcagttt atggcggggt gatttgctca catcgttctc
      961 cgttttttca tgatccaaca gttttatatc tcatcctgat ttgctccggt gttctgtttc
     1021 gacgttctca atgcctgcct ccccatagtg tgatccttat tgcaaaaatg aaaatccatg
     1081 ctcttgacat ggacaatgct gagtagtagg tttttactgt acataaaacc agtggttata
     1141 tgtacagtat attgttttta atttattgtt ttaattatca aagaggaatt tatggctaaa
     1201 gaactaagtg gatatggacc aactgctggt gagtctatgg ggggaacagg ggcgaatctg
     1261 aatcagcaag gtggaaataa taatagtaat agtggggttc actggggtgg tggctctggg
     1321 catggtaata atggtggtca gggaaattca aattcatctg gttctacatc aactgtgatg
     1381 aaaaccggtg agtcatatct gactccgtgg ggtgatgttg tcattaataa tgatggcctt
     1441 ccggtgatga atggtattgt tatgacagaa gagaattcaa ctctggttga taatccattt
     1501 ggaggggtct ccagagttct taattcttta attagtgata tgccatcact ttttgcagaa
     1561 agctcaggaa ataataacaa taacactgct tcagttaata ctgcaccaac gaatgctcag
     1621 gtcagtgata tggataagag ttcgaaggtg gtgggtaatg taattaatga aaaacaaaag
     1681 cagaagaata aaattgctac acagattagt gaaaaacaga agaaaatcga ggagatgaaa
     1741 aaagtattca agcatcattc ttatcatggt atcactgatc ttgagaggga tgttgatgag
     1801 ttgcaaaaaa aatcaaatca gcttgatgca gatattagca aacttaattc ttataaaaat
     1861 acattgcaaa gtaaaattgg tgatgtgaat aaacagaagg aagctgaaga aaaggctcgt
     1921 gaaaatgcag aggtcgctga acatgaaact cttaatgaag agaaacaggc ggtggcggaa
     1981 gcagagaagc gtttagctga ggcgaaagct gaactggcga aggctgaaag tgatgtacag
     2041 agtaagcaag ctactgtttc cagagttgca ggagagcttg aaaacgctca gaagagtgtt
     2101 gacgtgaagg tcaccggatt tccgggatgg cgtgacgttc agaaaaaact acagcgtcag
     2161 cttgaagcta agcaggctga gtactccgca gttgaaaatg aacttaagaa tgctgttagt
     2221 ttcagggatg gtaaggccgc tgaggttaaa gaggcagagc agaaattaaa agaagctcag
     2281 gatgctcttg aaaagagcca gataaaagat gctgttgata ctatggttgg attttatcaa
     2341 tatataaccg aacaatatgg agaaaaatac gcaaaaattg ctcaggattt agcggaaaag
     2401 tctaaaggaa agaaaattca aagtgttgat gaagctctgg cagcatttga aaagtataag
     2461 aatgtactgg ataagaaatt cagtaaggtt gatcgggatg ctatttttaa tgcgttagag
     2521 tctgttaatt atgatgagtt atctaagaat ttaacgaaga tatcaaagtc gcttaaaatc
     2581 accagtcgag tatcttttct atatgatgtt ggttctgatt ttaaaaatgc aatagaaact
     2641 ggtaactggc gacctttgtt tgtcacgctg gagaaaagtg cggtcgatgt aggtgtggca
     2701 aaaattgtgg cattaatgtt tagttttatt gttggtgtgc ctcttggttt ctgggggatt
     2761 gcaattgtca caggtattgt ttcttcttac ataggggatg atgagttgag caaacttaat
     2821 gagttgttag gggtttaatt tctctttaga gtgtaatata tgaaacttgg caatcctact
     2881 gggattgcca ttaataaata aaacaatgca gataacttgc ataagtacca gggttctttt
     2941 tttaactttg tattttcttt gatatatcta gatatggtta atttagagta tggatatagc
     3001 aatacatgta ttgatgatat aaatataaat aacaaaggag tgttatcatt gtcgttaaat
     3061 attaaaataa gaatccatgg tataagtgat tcaggtaaat tatgtaggta gtattttaaa
     3121 tgcataagta tttttacttt tgttggaaag aaatatgaaa ataataattt ttctaattac
     3181 aatattattc ttgtcaggat gtcaagcaaa ctatattcgt gatgtacaag gaggaaccgt
     3241 tgcaccatct tcgtcttctg aactgacggg gatcgcggtt cagtagaaaa gatcaaagga
     3301 tcttcttgag atcctttttt tctgcgcgta atctgctgcc tgtaaacgaa aaaaccgcct
     3361 ggggaggcgg tttagtcgaa ggttaagtca gttggggact gcttaaccgg gtaactggct
     3421 ttagtggagc acagatacca aatgctgtcc ttccagtgta gccgctataa ggcttcaact
     3481 tcaagagttc tgcgtatcta aatccactat ccagttacca atggctgctg ccagtggcgt
     3541 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3601 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3661 gagataccaa cagcgtgagc tatgagaaag cgccacgctt cccaaaggga gaaaggcgga
     3721 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc caccaggggg
     3781 aaacgcctgg tatctttaag tcctgtcggg tttcgccacc tctgacttga gcgtcggttt
     3841 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagctgcgt ggccttttcc
     3901 gggttcttgt tttttgctca catgtttctt ttggtgttat cccctaattc tgtggataac
     3961 cgcattaccg tctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcggagc
     4021 gagtcagtga gcgaggaagc ggaaaagcgc ctggacgagc attttctcct tacgcatctg
     4081 tgcggcattt cacacccggc atggtgcact tttcatacaa tcctcactga tgccaaatga
     4141 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     4201 cgctaaaacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gctcacaggc
     4261 cagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tccccgaagc
     4321 gtgcgaggca gctgcggtaa agctcgtcag cgtggtcgtg aagtgcttcg cagatgtcag
     4381 cctggtcgtc ggcgtccagt tcattcagtt tttccagcag ggtcagcgtc tggcttctga
     4441 tgaagcgggc catgttaagg gcggtttttt cctgtttagt cacagttctg tttctccatg
     4501 tgtggggagt ctgtcggagg tttttaatga taccggtgaa acgcgaaagg atgctcacga
     4561 tacgggtcac ggatgaggaa catgcgcggc tgctggcacg ttgtgaggga aaacaacttg
     4621 cgtcatggat gcgtaaggtc tgcctggggg caccgccatc gaaaacgtca ggtcttccga
     4681 cactggcacc gccgctgttg cgccagttcg cttcggtcgg caataacctg aatcagatag
     4741 cccggaagat aaacagcgga cagtggtcgg ggcatgaccg ggttcatgtg gtggctgcac
     4801 tgatggccat cggacgtgaa ctgagtgagc tgagggatga ggtccgtaaa cagggagaac
     4861 gggatgatag ttaagtttca tgccagggga aaaggcggtg gcagtggtcc ggttgattac
     4921 ctgctgggcc gggagcgtaa ccgggaaggg gcgagagttc tgcggggtgc tcccgaagag
     4981 gtgcgggaac tcatcgatgc cacgccgttt gcgaaaaaat acacctcggg ggttctgtct
     5041 tttgcagagc agaccctgcc accgggagag cgtgaaaggg tgatggagag ctttgagcgg
     5101 gtactgatgc ccggtcttga aaaaaatcag tacagcatcc tgtgggtgga gcaccaggac
     5161 aaggggcgtc ttgagctgaa ttttgtcatc ccgaatatgg agctggcgag cggaaaacgg
     5221 ctccagccct attacgaccg agcggaccgt ccgcgaatca atgcctggca gacactggtg
     5281 aatcaccatt acgggctgca cgacccgaac gcaccggaga acaggcggac acttgtcacc
     5341 ccgaataatc tgccgaaagc gaaacaggag gccgcagaag cgattacgag gggactggag
     5401 gctctttacc atgccggagc gctaaaaacg cgtcaggatg tcactgaggc gcttacagcg
     5461 gcaggttttg aggttgtgag gacaaccagg agcagtatca gtatcgccga cccggagggt
     5521 gggcgtaatc tcaggctgaa aggggcgctg tatgagcaat cttttacaaa tggggacaga
     5581 cttcgagaag aaactgaaag agcgtgccgt ctctacagag aacatgctga acagcgagtt
     5641 caggaagctc gaagcgtctg tagacaggga actgaactta aacgggcaga aaatcagaga
     5701 cgctatcagc gcacacacag cagcggtgaa agaacaactg gagacgctga acagtgccgt
     5761 gaacgcgcag ttcagcgcca cagagaacga actggcccgg cggcaggaag agctgctctg
     5821 gaagctggtc aaagggcgga tactgtaccc gtcgctgacg gcgctttgcg tcacgggcgg
     5881 gatttttctg gcgtcatggg ggctgattca gtggcaggaa agccggattg cggcgaacat
     5941 cctggctatc agggaccagg aagagacgct ggcgaaactc cgggaaaaaa catggggtgt
     6001 gacgtatcac gagggcagga acgggaaatt tctggtgttg ccgtccgggg tgaaggggga
     6061 aaacaactgg acggtggaga agaagaacgc agtaagactg gtcagggagt aagtgagtat
     6121 gacagagctg gaaatgcact tactgaacgc cttagagcaa ttgcagcagg actatacgca
     6181 gcggctgaaa gactgggaga gcgccttcac ggatttgcag aatatgttcg agctgacacg
     6241 acaggagaac gggcaactga gggaacagtg cgacgacttg agtcagcagg tgcagagatt
     6301 agccgggcag gtggaccact tgagtcggtt atttatcacg aacagcagat aagggatgaa
     6361 caggaaaggc aggcacgcct ggaacgggag cgtgcgcggt cctggcatga agagcaggaa
     6421 cgggaagcac gggtatggcg tggcccgtca atagggatgt gacggtgagt tgttccggga
     6481 ggttgccaga tggtaccgga acgcgttaaa aacgcatccg ggcgcgattc tggcgtgtcg
     6541 tttttgttgc tgtgttttgc ctctttgcca gatcgcgctc attcctcagc ctcatcccgc
     6601 gatgtctgcg tatgtcatca actgaaaaga tggaaaaaaa acagtgtcat gaggtttacg
     6661 caggcaaaac cagcgttatt cacatggctg aatagctatg catacggtta aaatttatca
     6721 ggtgcgatcg cggcagcttt tcgggtggtt tgttgccatt tttacctgtc agcagccgtg
     6781 atcgcgctga acgcatttca gcggtgcgta caattaaggg attatggtaa atcccttact
     6841 gtctgccctc gtatccatcg agataaaccg cagaaatggt gtctgccatt agtttcattt
     6901 ttttcggcga aggcatatcg ctgtttgtgt attttctgcc agcaaaggct gttgtgctcc
     6961 ggagtgagtg aaaaaatttt tctgagtgg
//
