LOCUS       NZ_CP010879             5167 bp    DNA     circular CON 01-JUN-2024
DEFINITION  Escherichia coli strain MNCRE44 plasmid pMNCRE44_3, complete
            sequence.
ACCESSION   NZ_CP010879
VERSION     NZ_CP010879.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03287565
            Assembly: GCF_000931565.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Hargreaves,M.L., Shaw,K.M., Dobbins,G., Snippes Vagnone,P.M.,
            Harper,J.E., Boxrud,D., Lynfield,R., Aziz,M., Price,L.B.,
            Silverstein,K.A., Danzeisen,J.L., Youmans,B., Case,K., Sreevatsan,S.
            and Johnson,T.J.
  TITLE     Clonal Dissemination of Enterobacter cloacae Harboring blaKPC-3 in
            the Upper Midwestern United States
  JOURNAL   Antimicrob Agents Chemother 59 (12), 7723-7734 (2015)
   PUBMED   26438492
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Johnson,T.J., Hargreaves,M., Shaw,K., Snippes,P., Lynfield,R.,
            Aziz,M. and Price,L.B.
  TITLE     Complete Genome Sequence of a Carbapenem-Resistant Extraintestinal
            Pathogenic Escherichia coli Strain Belonging to the Sequence Type
            131 H30R Subclade
  JOURNAL   Genome Announc 3 (2), e00272-15 (2015)
   PUBMED   25858844
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5167)
  AUTHORS   Johnson,T.J., Danzeisen,J.L., Hargreaves,M., Shaw,K. and Snippes,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2015) Veterinary and Biomedical Sciences,
            University of Minnesota, 1971 Commonwealth Avenue, 205 Veterinary
            Science, Saint Paul, MN 55108, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: CLC Genomics Workbench v. 7; HGAP v. 3
            Genome Coverage                   :: 125x; 100x
            Sequencing Technology             :: Illumina MiSeq 2x300; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000931565.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 04:01:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,301
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 99 of 250
            Pseudo Genes (incomplete)         :: 183 of 250
            Pseudo Genes (internal stop)      :: 39 of 250
            Pseudo Genes (multiple problems)  :: 60 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP010879.1.
            Source bacteria is available from Timothy Johnson
            (joh04207@umn.edu)
            Annotation modified by submitter.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MNCRE44"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMNCRE44_3"
                     /geo_loc_name="USA: Minnesota"
                     /lat_lon="46 N 94 W"
                     /collection_date="2012-04"
                     /collected_by="Health care provider"
                     /subtype="ST131 H30R"
     gene            82..996
                     /locus_tag="SY51_RS25330"
                     /old_locus_tag="SY51_25380"
     CDS             82..996
                     /locus_tag="SY51_RS25330"
                     /old_locus_tag="SY51_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271348.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(1200..1598)
                     /locus_tag="SY51_RS25335"
                     /old_locus_tag="SY51_25385"
     CDS             complement(1200..1598)
                     /locus_tag="SY51_RS25335"
                     /old_locus_tag="SY51_25385"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            1775..2701
                     /locus_tag="SY51_RS25340"
                     /old_locus_tag="SY51_25390"
     CDS             1775..2701
                     /locus_tag="SY51_RS25340"
                     /old_locus_tag="SY51_25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2704..3237
                     /locus_tag="SY51_RS25345"
                     /old_locus_tag="SY51_25395"
     CDS             2704..3237
                     /locus_tag="SY51_RS25345"
                     /old_locus_tag="SY51_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(3303..3512)
                     /locus_tag="SY51_RS25350"
                     /old_locus_tag="SY51_25400"
     CDS             complement(3303..3512)
                     /locus_tag="SY51_RS25350"
                     /old_locus_tag="SY51_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     Typing          83..857
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          1776..2698
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaatcatgtc cagatccggc aagattcaac atgaaataca gagggcgtgt aggtcgcata
       61 acccggagag aatcgtaaca catgagcgcc gcgcttcaat acttcgacga aaatttaccc
      121 catcgtccct atcacacgga tgatctcgct tttggtcttc gcatctccgg caaagggcgt
      181 gcgcttcttg cgcggtacat acagcagaac cagcctcatg cgcagttctg gctggttttt
      241 gacgttgacc gcgagggggc tgcgattgac tggagcgacc ggaacgctcc cgcgccgaac
      301 atcaccgtta aaaatcctgt gaacggacac gctcacctgc tctacgcact caacatcgcc
      361 gtgagaaccg cgcctgatgc ctcggttaag gcgctgaaat atgccgcagc ggttgaacgt
      421 tcgctgtgtg agaaactttg tgcggatgtg aactacagcg gcctgatttg caaaaatccg
      481 ttccacctgg aatggctggt gatggagtgg cgcgaggaag cctataccct cgatgaactg
      541 gctgattatc tcgatttgag cgcctcagag cgtcgtagca tcgataaaca ttacgggatg
      601 gggcgaaact gccacctgtt cgaaatgacg cgcaaatggg cttacagggc gattcgtcag
      661 ggctggccag cgttctcaca gtggcttgaa gcggtgatcc agcgtgtcga aatgtacaat
      721 gcatcgcttc ccgttccgct ttcactggct gaatgtcggg ctattggcaa gagcattgcg
      781 aaatacacgc acaggaactt cacgccggaa actttcgcac agtatgtggc tgatacgcac
      841 acgccagaaa ttcaggctgc acgcggtcgc aagggcggtt ctaagtctaa gcgcagcaca
      901 gtagctacat ctgcacgcac gctgaaaccg tgggaggcat tagggatcag tcgcgcctgg
      961 tattaccaac tgaaaaaacg aggtctcgta gagtagacca aataagccta tatcagataa
     1021 cagcgccttt ctggcgtctt tttgagcagc aggttttttg ccgtttttcc ccgttcagcc
     1081 ctgcaatggc gcggctttcc gcgtgattgt ggtggtagtg ctcgccgcag tctcatgacc
     1141 gagcgtagcg agcgaatgag cgaggaagca ccagggaatc ggaacacgga tggaggcact
     1201 tacgcgtctg ggtttgggga tgtctccaga tcgtacttaa gttccttaag tgcttgtaat
     1261 atcctgtctt ttttatagca tagctcgcct gtttttgtgt ctaccagtcc tgcttcgatc
     1321 agtagcccag ccgcgagaaa gagccggtga ttgcgttccc gtcgttgttt ttttccctcg
     1381 attcgttttt ggaccgcctg ttttcgttcg ctctcctgtg cctgtaagtc gtttttttcc
     1441 gctgttgcca gtacgtggag aagtttgcgt tcctgttctg tgagtccggc ttcgttgtcg
     1501 atcaggtcaa taatctgtct ctgtcgtgcg gtctgttctc cgagcttcct gatgaatccg
     1561 atccgtttct tcagccattc gatgttgctg atgcgcatgt tcgtgatgct ccctggtgtt
     1621 tttttgcggt gtgttgtggt cgtgcagtat atcgcttgcg gtcggcgggt gcagggggta
     1681 tcttccgtgc taattcgtaa aattagaagg aagcgcactt atatgtttct ctccttcgtc
     1741 gagaaacagt gcgctcctgc agagggctga aaccgtggca acctatcacc tcagcgttaa
     1801 atttggtggc aaggggcagg ctgcaaacca cgccgattac atcgagcgca aagagaaata
     1861 ccgcgaccgt caggatttgg aatattccgc acacggaaac atgccggaat gggccaggga
     1921 taatccttcc catttctggc aggcggctga ccagtttgaa cgtgcgaacg gttcgaccta
     1981 tagggaactt gaaattgcat taccccgtga actgacaccg gaacagcgcc ttgaactggt
     2041 gcaggatttt gtacgtcagg aagctggcga acggcacgcc tggagttttg ccattcacaa
     2101 tccgaaagcg agcattgacg gcggcgaaca gcctcatgcg catatcatga tgagtcagcg
     2161 tgtgaatgat ggcattgaac gtacaccgga acagtatttc cggcgctata acgccaggta
     2221 tcccgagcgg ggaggtgcga aaaaggattc cggaagcctg actctcactc agcaaaaaga
     2281 gcagttgcgg gaattgcgaa aacgctggga ggtgaagcac aacgaacata tgagaaagca
     2341 tggctttgaa aggggcttca ttgactgccg gacgctgaaa gaacagggca ttgaacgacg
     2401 tcccgaagtg catctgggtt ttgaagctgc cggacggctt gatgatgccc agcgtcatga
     2461 catcatgcaa aaacggtctg aaccggacta ttcccgccat cttgaacagg cgcgaacggt
     2521 cgggcatgac atcagggaat ggctggacac tccggttaag caacatgaac tggtcagaag
     2581 gctgcaacat gatgttgcgc gggagcatgg ctttgacatg agagacagag tgagcagaca
     2641 gaaccgaact gcacctgaac gggttaaatc accgtcacgt ggattttcta tgggattctg
     2701 aacatggcca gcacagaaga aaaattactt acgctcctgt tatcggtgga gcatctgcaa
     2761 cataccgcaa tggagcaaca gaaagctgtt gctgattctc tggctgtggc aagggaaact
     2821 cttcgcggat atcgggatat ttctgacggt gtggcaaaac aggtcaggga tgaagtcaga
     2881 aatgaggtgc agaagctgga cgtggcagga ctgataggtg agcgcatcag ccaggatttt
     2941 cagcggctgg aagagtcagt cgcaaacatg aaggcaaacg cggatgccct gaacagggat
     3001 attgcgacaa ttcacagcaa tatcgtgcag gaatacaaca cccttaaagg gtatagctgg
     3061 aaatggctgg gtggggtgtc agtggctttt ctggttatgt tctgtgtgct cggatgggga
     3121 atgaaaacaa tactcgacag gaactacaac gccacagcgg cggtgtatca gaaagttgtg
     3181 acactggaag ataaactggt ggagaaacct gtcacgggga agagccggaa aaactgacgc
     3241 gcttcgcttg tccaacgcct cacgacccgc gaaagaattt cgcgaaccgt tcaccgtttt
     3301 tttcaatatg attctgtctg ctttggtcga taaagcagac gatagtattc cagccttcgt
     3361 atatataacg gtacgtgctc atcagtaaga gctggcggta ttgtgttatc agggggaagt
     3421 tttttcagat actcacgaaa tgcctggctt gatgccataa aatctacagt agcatcgata
     3481 atatgtgggg tgttttcacc taattttgcc atgatgataa tccttatatt atcacggttt
     3541 gtgaatgata gggtgtcact attatttatg cgttagtgaa ccagaaaaaa tcccccgttg
     3601 gcataggggg atatgtaagg agaaataact tcgcaggtgt tcttgtttgt ataacgtgaa
     3661 aagactgcac gcaacttaat aagtctgccg tacttacttt cagttttcaa cataaggttt
     3721 ataacaatgt atggaatgga agtgagcttt aaggtttcag ctctaaaaaa taaaatattt
     3781 attagttggc tggttgttgt tcttgttctg tatgtatata aacactatcg acatattatg
     3841 accgggaaca ttccttaccc cctctgcgct cgcagaggtt ttttttgtca ttaattaata
     3901 atgaactgaa atgcagttcc gatacctcca tttgcacatt atccctgaca tatggtaccg
     3961 gaaccgcatt aattttatgt tataagagtt tatttttaat gtccagatgg tttaatagta
     4021 tttatatgta attagctgta tggatagtac tggttttatt gtataacttg tcgttttttt
     4081 ttattgtttt tgtgtggtgt gctaatcttt aagttataaa aaaacccctt ggaatacagg
     4141 ggcaaaagta aggaaaacaa atggatgctc agtttgtgtg acactttaaa ctaatggaac
     4201 tatagagtgg aggttgttac agtcgctttt ataaaacagc aaccagtttt tttcaatagt
     4261 ctgatgatgg ttgtgacagt tttgtgatat tgttcaaaat gtttcttgtt gaatgtttgt
     4321 tttttggtct attggtgtct ggttttattt tttctgctat tacatatcaa gaaatgtgat
     4381 tggtgtagca agagtggaat gcgatgtgga taaactaatt ggttgtcgaa tctaatttac
     4441 ttagaaaact acacttgttt ttgtgtatga atttagtgaa tttgtctgtc tatgtgtttt
     4501 cgtgcattta taaatccttc gtcaggatct taaatttagt gttgaatatg tagggcctgt
     4561 acggtaatta ttatttagcg tagctacttc atcgttgttc gaacacaacc aacaacctgt
     4621 aattgttgtt tctcttggtt ccccactttt gtggggtttt ttatctcttc cgtgcatcgt
     4681 aaagcgcgga ctccttctgg aaaggttgtt ttagggcatc actgaggcat tttttgcgtt
     4741 tgtgaatatt gagcatccga taactaaaat atttcccata gactctgatt cttgccaccg
     4801 accccaggtc ggtttttttt tgtcctggcg agatgtgaag tttggaaaca ggaaaccccg
     4861 tcagagcgcc acgcaccagg ggagaaaact tgtcgttttg agccgttcgg tgttcagaac
     4921 gcacgaaacc gatcgcacat cgctttcgtg catagttatg caacgccctg aaaacgattc
     4981 tgacgcgttt ttctggtttg gcctggtgtt ttcttgtctt tttgcgtttt ttgcgccaga
     5041 aagcgactga gggcgtttta aggggggcgt acaacgggag ttatggtaaa tggatcgggt
     5101 tttcgggaag gttcgacagg atttgccgtt gggtgtagtg taagcgactg aaaaacaaac
     5161 gcccctg
//
