LOCUS       NZ_CP010877             1506 bp    DNA     circular CON 01-JUN-2024
DEFINITION  Escherichia coli strain MNCRE44 plasmid pMNCRE44_1, complete
            sequence.
ACCESSION   NZ_CP010877
VERSION     NZ_CP010877.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03287565
            Assembly: GCF_000931565.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Hargreaves,M.L., Shaw,K.M., Dobbins,G., Snippes Vagnone,P.M.,
            Harper,J.E., Boxrud,D., Lynfield,R., Aziz,M., Price,L.B.,
            Silverstein,K.A., Danzeisen,J.L., Youmans,B., Case,K., Sreevatsan,S.
            and Johnson,T.J.
  TITLE     Clonal Dissemination of Enterobacter cloacae Harboring blaKPC-3 in
            the Upper Midwestern United States
  JOURNAL   Antimicrob Agents Chemother 59 (12), 7723-7734 (2015)
   PUBMED   26438492
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Johnson,T.J., Hargreaves,M., Shaw,K., Snippes,P., Lynfield,R.,
            Aziz,M. and Price,L.B.
  TITLE     Complete Genome Sequence of a Carbapenem-Resistant Extraintestinal
            Pathogenic Escherichia coli Strain Belonging to the Sequence Type
            131 H30R Subclade
  JOURNAL   Genome Announc 3 (2), e00272-15 (2015)
   PUBMED   25858844
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1506)
  AUTHORS   Johnson,T.J., Danzeisen,J.L., Hargreaves,M., Shaw,K. and Snippes,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2015) Veterinary and Biomedical Sciences,
            University of Minnesota, 1971 Commonwealth Avenue, 205 Veterinary
            Science, Saint Paul, MN 55108, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: CLC Genomics Workbench v. 7; HGAP v. 3
            Genome Coverage                   :: 125x; 100x
            Sequencing Technology             :: Illumina MiSeq 2x300; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000931565.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 04:01:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,301
            CDSs (total)                      :: 5,188
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 99 of 250
            Pseudo Genes (incomplete)         :: 183 of 250
            Pseudo Genes (internal stop)      :: 39 of 250
            Pseudo Genes (multiple problems)  :: 60 of 250
            Pseudo Genes (short protein)      :: 1 of 250
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP010877.1.
            Source bacteria is available from Timothy Johnson
            (joh04207@umn.edu)
            Annotation modified by submitter.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MNCRE44"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMNCRE44_1"
                     /geo_loc_name="USA: Minnesota"
                     /lat_lon="46 N 94 W"
                     /collection_date="2012-04"
                     /collected_by="Health care provider"
                     /subtype="ST131 H30R"
     gene            complement(join(1208..1506,1..361))
                     /locus_tag="SY51_RS31655"
     CDS             complement(join(1208..1506,1..361))
                     /locus_tag="SY51_RS31655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021534844.1"
                     /translation="MCKCNTVTRDDIPFAIWSGLHPDTPHQCVRAQCGFRFSSASSPTR
                     SLTFGRWGCGERCHLSHVIGDSAVVLSGMSRQIALQTLSEALRRTKRVLNARQGMEGAK
                     NAYRGFQEVRRADTPNQGLSGRQEPRQPPAKPICTALAAIQLVGGRRPSMLQRWGSGVL
                     PRRFPVALKALPEPWQLRRFGDYRAKVANDVNDVILRRLPYPASHRTDMRQKSNAG"
                     /name="hypothetical protein"
     gene            710..1150
                     /locus_tag="SY51_RS25310"
                     /old_locus_tag="SY51_25360"
     CDS             710..1150
                     /locus_tag="SY51_RS25310"
                     /old_locus_tag="SY51_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000031238.1"
                     /translation="MSKDVERITQINDQGEIIGGFVAVIRPKQKSAFQRHFTMNQDALR
                     ILAKELTGEQFKVLMLMLADLDYENFIQIAQADIAETLGMQKTNVSRAVRALLDVGVIF
                     EGPKVGRSKTYRLNEQFGWKGTVTNHKKALKNGFSVIQGGRI"
                     /name="hypothetical protein"
ORIGIN
        1 ggttaggggt atcggcgcgc ctgacctcct gaaaccctct gtaagcgttt ttcgcgcctt
       61 ccatgccctg acgggcattt aaaacccgtt tcgtgcgcct gagagcttct gagagcgttt
      121 gaagagctat ctggcgactc atccccgaaa gaacaacagc ggaatcaccg ataacatgcg
      181 agaggtggca ccgctcgccg cagccccagc gaccgaacgt tagtgagcga gtgggcgagg
      241 aagcggaaga gaaccggaaa ccacattgag cacgtacgca ctgatgcggg gtgtcggggt
      301 gaagccctga ccagatggca aacggaatat cgtcgcgggt gacggtatta catttgcaca
      361 tcctgtcccg atttctgcgg agtttaacgg tgaacggacg gcaaaaaacg tgcaggagct
      421 gttgaacgtg aaaacctcga aatctggcaa aaatcacggc tgacgaggct tgcagaggat
      481 taagccagta tacactccgc tatcgttacg tgactgggtc agggcttcgc cccgacaccc
      541 caaagagtag ctaggataaa tatctaatgg aatcaatgcg ttagacgcct aatataaaga
      601 tacagaaagg agaataaaaa cattaacaaa atctaagggt tacagttgac atagttatca
      661 ataatgataa cactgttatc attattgata actcaaatag ggttcaaaga tgagtaagga
      721 tgtcgagaga atcactcaga taaatgacca gggtgagatt atcggcggct tcgttgccgt
      781 tatccgccct aaacaaaaat cagcatttca gaggcatttc accatgaatc aagacgcatt
      841 acggatcttg gctaaggaat taaccgggga gcaattcaag gtcttaatgc tgatgcttgc
      901 agatctggat tacgaaaact tcattcagat cgcccaagca gatattgctg aaactttagg
      961 gatgcagaaa acgaatgtta gtcgcgccgt tcgcgcgcta ctggatgttg gggtgatctt
     1021 tgagggacct aaagttggca gatcaaaaac ataccggctg aatgaacaat ttggttggaa
     1081 aggcaccgta accaaccaca aaaaggcact caaaaacggc tttagtgtca ttcagggcgg
     1141 ccggatttaa agagccagcg gcaggcggac aatcagggct acgtgttaac gttttgacca
     1201 tgattgtcta tcctgcattg ctcttttgcc gcatgtcagt tcgatgcgat gcaggatagg
     1261 gcaaacgccg caaaatgacg tcattgacgt cattcgcaac ctttgcccga taatctccaa
     1321 aacgtctgag ctgccacggt tccggcagcg ccttgagcgc aaccggaaac cgtctgggga
     1381 gaaccccaga cccccatcgc tgtaacatcg acggtctacg accaccaacc agctgaatag
     1441 ctgccagtgc cgtgcaaatg ggctttgcag ggggctgacg cggttcctgg cgacctgaaa
     1501 gcccct
//
