LOCUS       NZ_CP010832             8405 bp    DNA     circular CON 03-MAR-2024
DEFINITION  Shigella sonnei strain FORC_011 plasmid pFORC11.3, complete
            sequence.
ACCESSION   NZ_CP010832
VERSION     NZ_CP010832.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03284775
            Assembly: GCF_001518855.1
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Lee,J.-H., Lee,D.-H., Kim,S., Ku,H.-J., Chung,H.Y., Kim,H., Ryu,S.
            and Choi,S.H.
  CONSRTM   Food-borne Pathogen Omics Research Center
  TITLE     Complete Genome Sequence of Shigella sonnei FORC_011
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8405)
  AUTHORS   Lee,J.-H., Lee,D.-H., Kim,S., Ku,H.-J., Chung,H.Y., Kim,H., Ryu,S.
            and Choi,S.H.
  CONSRTM   Food-borne Pathogen Omics Research Center
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2015) Food Science and Biotechnology, Kyung Hee
            University, 1732, Deogyeong-daero, Giheung-gu, Yongin, Gyeonggi
            446-701, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: CLCbio CLC Genomics Workbench v. 7.5; PacBio SMRT
            Analysis v. 2.3.0
            Genome Coverage                   :: 316.02x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/03/2024 09:10:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,249
            CDSs (total)                      :: 5,120
            Genes (coding)                    :: 4,408
            CDSs (with protein)               :: 4,408
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 712
            CDSs (without protein)            :: 712
            Pseudo Genes (ambiguous residues) :: 0 of 712
            Pseudo Genes (frameshifted)       :: 206 of 712
            Pseudo Genes (incomplete)         :: 508 of 712
            Pseudo Genes (internal stop)      :: 122 of 712
            Pseudo Genes (multiple problems)  :: 117 of 712
            Pseudo Genes (short protein)      :: 3 of 712
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP010832.1.
            Source strain is available from the Food-borne Pathogen Omics
            Research Center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8405
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="FORC_011"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /plasmid="pFORC11.3"
                     /geo_loc_name="South Korea"
                     /lat_lon="37.53 N 126.96 E"
                     /collection_date="1999-03-20"
                     /collected_by="National Culture Collection for Pathogens,
                     South Korea"
     gene            complement(join(7855..8405,1..25))
                     /locus_tag="FORC11_RS33755"
                     /old_locus_tag="FORC11_p0373"
     CDS             complement(join(7855..8405,1..25))
                     /locus_tag="FORC11_RS33755"
                     /old_locus_tag="FORC11_p0373"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="WP_094190806.1"
                     /translation="MTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLA
                     AFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLAS
                     GGIGMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAW
                     IAGAALYLLCLPALRRGLWRNSSNSRCT"
                     /name="MFS transporter"
     gene            complement(278..652)
                     /locus_tag="FORC11_RS33760"
                     /old_locus_tag="FORC11_p0365"
     CDS             complement(278..652)
                     /locus_tag="FORC11_RS33760"
                     /old_locus_tag="FORC11_p0365"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_237158205.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALMQFACAPVLGRAVGSFRAAPVLLVSLAGAAVDYAIMATAPFLWVLYIGRI
                     VAGITGGDWGGSRRLYCRYH"
                     /name="hypothetical protein"
     gene            758..1408
                     /gene="tetR(A)"
                     /locus_tag="FORC11_RS26980"
                     /old_locus_tag="FORC11_p0366"
     CDS             758..1408
                     /gene="tetR(A)"
                     /locus_tag="FORC11_RS26980"
                     /old_locus_tag="FORC11_p0366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            complement(1440..1683)
                     /locus_tag="FORC11_RS26985"
                     /pseudo=""
     CDS             complement(1440..1683)
                     /locus_tag="FORC11_RS26985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /name="relaxase"
     gene            complement(1989..2825)
                     /locus_tag="FORC11_RS26995"
                     /old_locus_tag="FORC11_p0368"
     CDS             complement(1989..2825)
                     /locus_tag="FORC11_RS26995"
                     /old_locus_tag="FORC11_p0368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(2825..3628)
                     /gene="aph(3'')-Ib"
                     /locus_tag="FORC11_RS27000"
                     /old_locus_tag="FORC11_p0369"
     ARG             complement(2829..3628)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(3689..4504)
                     /gene="sul2"
                     /locus_tag="FORC11_RS27005"
                     /old_locus_tag="FORC11_p0370"
     ARG             complement(3693..4504)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(4964..5122)
                     /locus_tag="FORC11_RS33965"
     CDS             complement(4964..5122)
                     /locus_tag="FORC11_RS33965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            5693..5842
                     /locus_tag="FORC11_RS33765"
                     /old_locus_tag="FORC11_p0371"
     CDS             5693..5842
                     /locus_tag="FORC11_RS33765"
                     /old_locus_tag="FORC11_p0371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(6609..7049)
                     /locus_tag="FORC11_RS27010"
                     /old_locus_tag="FORC11_p0372"
     CDS             complement(6609..7049)
                     /locus_tag="FORC11_RS27010"
                     /old_locus_tag="FORC11_p0372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            7267..7656
                     /locus_tag="FORC11_RS30595"
     CDS             7267..7656
                     /locus_tag="FORC11_RS30595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            7700..>7888
                     /locus_tag="FORC11_RS30600"
                     /pseudo=""
     CDS             7700..>7888
                     /locus_tag="FORC11_RS30600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     ARG             complement(1993..2825)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(7856..8403)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="42.92"
     Typing          5485..5658
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(4956..5561)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ccatcagggc ggcgacgacg gtcatgcccc gggcccaccg gaacgaagcg agcgggttga
       61 gagcctcccg gcgtaaacgg cccggcgttc gcctttgtgc gactccggca aaaggaaaca
      121 gcccgtcagg aaattgaggc gttcaaggct gcccgcggcg aagaacggag cgtgggggga
      181 gaaaccgccc atcagcccac cgagcacagg tcccgcgacc atcccgaacc cgaaacaggc
      241 gctcatgaag ccgaagtgcc gcgcgcgctc atcgccatca gtgatatcgg caatataagc
      301 gccggctacc gccccagtcg cccccggtga tgccggccac gatccgcccg atatagagaa
      361 cccaaaggaa aggcgccgtc gccatgatgg cgtagtcgac agcagcgccg gccagcgaga
      421 cgagcaagac cggcgccgcc cgaaacgatc cgacagcgcg ccccagcaca ggtgcgcagg
      481 caaattgcat caacgcatac agcgccagca gaatgccata gtgggcggtg acgtcgttcg
      541 agtgaaccag atcgcgcagg aggcccggca gcaccggcat aatcaggccg atgccgacag
      601 cgtcgagcgc gacagtgctc agaattacga tcaggggtct gttgggtttc acgtctggcc
      661 tccggaccag cctccgctgg tccgattgaa cgcgcggatt ctttatcact gataagttgg
      721 tggacatatt atgtttatca gtgataaagt gtcaagcatg acaaagttgc agccgaatac
      781 agtgatccgt gccgccctgg acctgttgaa cgaggtcggc gtagacggtc tgacgacacg
      841 caaactggcg gaacggttgg gggttcagca gccggcgctt tactggcact tcaggaacaa
      901 gcgggcgctg ctcgacgcac tggccgaagc catgctggcg gagaatcata cgcattcggt
      961 gccgagagcc gacgacgact ggcgctcatt tctgatcggg aatgcccgca gcttcaggca
     1021 ggcgctgctc gcctaccgcg atggcgcgcg catccatgcc ggcacgcgac cgggcgcacc
     1081 gcagatggaa acggccgacg cgcagcttcg cttcctctgc gaggcgggtt tttcggccgg
     1141 ggacgccgtc aatgcgctga tgacaatcag ctacttcact gttggggccg tgcttgagga
     1201 gcaggccggc gacagcgatg ccggcgagcg cggcggcacc gttgaacagg ctccgctctc
     1261 gccgctgttg cgggccgcga tagacgcctt cgacgaagcc ggtccggacg cagcgttcga
     1321 gcagggactc gcggtgattg tcgatggatt ggcgaaaagg aggctcgttg tcaggaacgt
     1381 tgaaggaccg agaaagggtg acgattgatc aggaccgctg ccggagcgca acccactcac
     1441 tacagcagag ccatgtagac aacatcccct cccccctttc caccgcgtca gacgcccgta
     1501 gcagcccgct acgggctttt tcatgccctg ccctagcgtc caagcctcac ggccgcgctc
     1561 ggcctctctg gcggccttct ggcgctcctg ctgcggcgtc cgctcgtggg ccgtggcgcg
     1621 ggtccgcgcg ccggcctcgt gcgcctggcg ctcgcgggcg aggtccaggg cggccgtctt
     1681 cacgttctgc cttgcgcaga tgagatagat ctagcgtgga ctcaaggctc tcgcgaatgg
     1741 ctcgcgttgg aaactttcat tgacacttga ggggcaccgc agggaaattc tcgtccttgc
     1801 gagaaccggc tatgtcgtgc tgcgcatcga gcctgcgccc ttggcttgtc tcgcccctct
     1861 ccgcgtcgct acggggcttc caggggtcgt ttgcgggaag gggcggaatc ctacgctaag
     1921 gctttggcca gcgatattct ccggtgagat tgatgtgttc ccaggggata ggagaagtcg
     1981 cttgatatct agtatgacgt ctgtcgcacc tgcttgatcg cggccgcgat agctagatcg
     2041 cgttgctcct cttctccatc cgcgttccaa gctgcggaaa ggcacccata agcgtacgcc
     2101 tggtcgagca ggcgacgcgg atcgacgtcc agcgcacgag agaatgcgtc cgccatctgt
     2161 gcaatgcgtc taggatcgag acaaaggtcg tctctgtcag ccggatcgta gaacatattg
     2221 gcggcgccaa agcccacttc accgaccaga ccgacgggat ctatcaccag ccagccgcga
     2281 ctggagaaca tgatgttttc atgatgcaga tcgccatgta gcccacgcag ttccgaggca
     2341 ttgctcatca tttgatcggc tataatcgcc gcgtggacgt agtcagtttg acaacctgcg
     2401 ttttgatcat cgcgcgcccg ctgaaacaaa gctgcaaagc gatcccggat cgggagaagg
     2461 gcagaaggca ggggttcctc agatgcggca tacagcttcg ccattagttc cgctgcaatt
     2521 tcggtcgcct ggtagtcgcc gtgctcggca acgatgtgag agagcattcg ctccccggca
     2581 tattcgagca acatcagatt gttctcacga ccgagcaacc ggactgctcc cctcccattg
     2641 cgccatacca gatagtcggc cccgcgcagt tcatcagcaa tgtcttctat aggtttcaat
     2701 cccttgacga ttgcaggagt cccgtctggc aatgaaactt tccaaacgag gctggaaaag
     2761 gtgtccgcaa tgagaacagg ttgcgaaacg tgccaatgag caggaaaaac aggcggcatg
     2821 aacatcaacc ccaagtcaga gggtccaatc gcagatagaa ggcaaggcgt tcgcggtcgg
     2881 gggcttcgat ccccaataca ttgaatagga cagcgaaggc gcgctctgct tcatctggcg
     2941 ctgcccagtt ctcttcggcg ttagcaatca tgagtgccaa atcggcatag cgatctgctg
     3001 ttccgagccg cccaaggtcg atcagacccg tgcattgaag agttttaggg tccaccatga
     3061 agttcggcat gcagggatca ccatggcaaa caaccatatc ggtgcgctct tggtcgagcc
     3121 gcaccggtag ctctcgttcg acacgagcca aaagatcgag ctgcggcgta ctcttgtcct
     3181 cgtccggtaa gaagtcggga ttgacggcat tgcgggacac cacatcaacg gcgcgtccga
     3241 acattcgcga cagcctgcgc tcaaacggac attgatcaac cgataggctg tgaacagcgc
     3301 caagttgctg ccccattgac ggccacgctt tgagcaaatc cgctccagac agatcagccg
     3361 ccggtactcc cggaattgcc gttatcacca agcatgcacc ctcctgttcc tcctgccagt
     3421 tgatgacctc ggggcaagcc acacctcgac ctttgagcca aatgaggcgg tcacgctctc
     3481 cagcgagctc accgcggcgg gaagcaggtg cgattttcgc gaaggcatgc ccgtcaccac
     3541 gtcgaaaaac aaaatcacca gattctccgc ctctgacagg caaccagtca gaatgcgatt
     3601 caccaaaaaa aatattagtt cgattcaatg gaggttcctt cagttttctg atgaagcgcg
     3661 aatatagaga aatatcccga atgtgcagtt aacgaattct tgcggtttct ttcagcgccg
     3721 ccaataccgc cagcccgtcg cgcaaggggc gcggctcgtg tgtgcggatg aagtcagctc
     3781 cacctgcggc ggcggcaagc tctgcagcga gtgtcgcggc cccgacatcc cccggaccac
     3841 ggcctgtgag cgcgcgcaga aaggatttgc gcgaaacaga cagaagcacc ggcaaatcga
     3901 agcgcagccg caattcatcg aaccgcgcca gcaccgagag cgaggtttcg ggagcagccc
     3961 ccagaaaaaa ccccatgccg ggatcaagga caaggcggtt gcgtttgata ccggcacccg
     4021 tcagcgccgc gatgcgcgcg tcaaagaacg ccgcaatgtg atccatgatg tcgccagcgg
     4081 gtgcctcgcg ccgatctgcc tgcccgtctt gcaccgaatg cataacgacg agtttggcag
     4141 atgatttcgc caattgcgga tagaacgcag cgtctggaaa accgcgaata tcattgagat
     4201 aggccacacc acgcgacaag gcataggctt gcgtcgcggg ttgataactg tcgagcgaga
     4261 cgggaatgcc atctgccttg agcgcgtcca gcaccggcgc gatacgcgcg atttctgtgt
     4321 cggacgaaac aggcgcggcg tcgggattgc tggatgccgg accgaggtcg atcacatctg
     4381 ccccctcggc catcagctta cgcgcctgcg caatggctgc gtctggcgcc agataccggc
     4441 ctccatcgga gaaactgtcc gaggttatgt tgacgatgcc gaaaatgatg agcgatttat
     4501 tcatgggggc ttctataata ataataatcg agcatgagtc tcatacggat gctcgggtcg
     4561 aaagggaatc cccaggcgag taacctgttt gcggtgatcc gtttcatgat gatgtatctg
     4621 tagcattttg gaaataaata tatcatctct caataagtag atatatttat cttttttgct
     4681 tctttggtgc tgcaagtcac caatacgagg gaaacctgtt acggagcggt ttaaccgtaa
     4741 tcaacagtga ctggtgtcat gttcggaaga ttaattgata gtaatctaca aaggatcttc
     4801 ttgagatcct tttcaatgaa tcgtaatctc ttgctctgta aacgaaaaaa ccgcctgggg
     4861 aggcggtttg ttcgaaggtt aagtcagttg gggaactgct taacccggta acagggttca
     4921 cgagaccgcc attaccaaaa ctgttctttc agtgtagccg cagtcaggcc tctacttcaa
     4981 gacctcttaa tctatcttct cgtgcactcc tgttactaat ggctgctgcc agtggcgttt
     5041 tgtcgtgtct ttccgggttg gactcaagtt gatagttacc ggacaaggcg cagcggtcgg
     5101 actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgccttc acggaactga
     5161 gtgtcaggcg tggaatgaga aaccgcggcc ataacagcgg aatgacaccg gtaaaccgaa
     5221 aggcaggaac aggagagcgc acgagggagc taccaggggg aaacgcctgg gatctttata
     5281 gtcctgtcgg gtttcgccac cactgatttg agcgtcagat tttgcgatgc ttgtcagggg
     5341 ggcggagcct atggaaaaac ggcttcgccg tggccttatc tcttctttgc taagtgtctt
     5401 cctggtatct tccaggaaat ttccgtcccc gtccgtaagc cggtaccgct cgccgcagcc
     5461 gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga atatctcctg tatcgcatat
     5521 tctgctgacg cgccggtgct gccttttttc tcctgccaca tgaagcactt cactgatttt
     5581 cgcaatcgtg gcaacatagt aagccagtat acactccgct agcgctacat gactggttca
     5641 gggctgcgcc ccgaaacccg ctaacaacct ctcagctact ggatatcacc ccatgccatc
     5701 tgaatatgat gaacaactgg attcgactga tacgcagctt cccggcgaat atgacctttt
     5761 tgatttaatg gcgcaggtta ctgaagagaa cctgcatgcc cctgtggatt ttgggtcgcc
     5821 tgtcggccgg gaagcgttat agctttttcg cttgcccgcg tacgtggtaa tactccggta
     5881 ttacctttta tttctttatt ttcatgtaat tacagtggat ttttaatacc cgggtattac
     5941 tcgtgaaaac gaaatattcc ttttttttct tttaattcat gcagttgcga tgaaaatcgc
     6001 ggctgcgtta ggtgtatgac cattttgagg ttgaaaaata atcaaccatg gcgggtgatt
     6061 tacgcaggtt aaggggagat cgtgacaaaa aaagcgttcc tcactgtatc gttcatgcct
     6121 gcatgaacgg gttattcttc ctgactacag aattacatcg ctccttcagt tcatacatca
     6181 cttttacatc acttatacat cacttataca tcacttatac atcactttta catcattcct
     6241 tcagctcata cgtcactttc atcctctgtg ccatttgtac atcaccccct tcctgaacgt
     6301 gctgacagcg ggttttccgg ctgcgccggc aacgcttcac ggccacgcaa taaattgcgt
     6361 ccccgttcac cgttttcgga tgggaagaac gccggaaaca ggacttaccc cggctgaatg
     6421 gtgtgaattt ccgcgctatg cacttgtgcg catactcatg catgccgtaa aaacagagcc
     6481 tgcgcgtttc tggcgggttt tcgggtggtt tgttgcctgt tttaccggtt tcccgtcaga
     6541 aacgccctga gggcctctca ggcggtgctg ataacagatg ttatggtaaa tcagagtcac
     6601 ctgcactgtt actctgaacc ggagcgaaac agccgcgacc agaatccccg ctttttttcc
     6661 tgtgccagcg cgtcagccag acggctaatt tcggcctgta gttgttccct ctcctcattt
     6721 cgcaggcggt tttctgcttc ccgcgccatc ttgtcttcaa gcattagggt aacagtctgc
     6781 tgcagagcgt ccagtttctg ctgaatgacg tctagctgag cattttccgc ctggctaacg
     6841 acagatttgg agtgtccctc cttgtgcctt tttggtgtct cagaagtgcg cagctcgcca
     6901 taggcccgaa tcagttcact ggtctcgaac tcccggcgat cattttttcc tgtgcgatag
     6961 ctgacaaggc ctttagccat gtcacgatat aattgcgagc gcgactttcc tgttaatttc
     7021 agtgtctctt gaactgtgtg ccatgccata aaagtgacag tgtctcctgt gtgtaccgta
     7081 ttgtaacagc agaacatatt actgttttaa cacaaaaggt ctgaggctgc ccgtgaggcg
     7141 tgaaaaatac acaaaaaacc tgttgacggt gtggttttga cgggcgtagt atttcgaata
     7201 ctgtatataa acacattgtt ataaatacag tttttgctgt ttttaaaagt caaagaggat
     7261 aattttatga gtacagagtt aaatagcggt actgtcggtg gttttggtca catgggacca
     7321 aatgacccct acaatgatgg tcatagcggc tctggaagtt cgggaggtag ttttaacggt
     7381 ggtggtaatg gtggccatga taatagtagt ggttcatctt atactgcatc ctttggtctc
     7441 atcactggcc gacacccgat aactgtccca ggtacgaaga atattaattc tgtcgatctt
     7501 tctgctgtca ttaataacat tatacatcat gaaggtgcac gcaataacat gtacctagat
     7561 actgcgaaca atgtaagagt gggagtgacg ggcactggct ggcaatgtct agcaacggca
     7621 ggcatttcgg ctgagggtaa aagaactttc cgctaagcga tagactgtat gtaaacacag
     7681 tattgcaagg acgcggaaca tgcctcatgt ggcggccagg acggccagcc gggatcggga
     7741 tactggtcgt taccagagcc accgacccga gcaaaccctt ctctatcaga tcgttgacga
     7801 gtattacccg gcattcgctg cgcttatggc agagcaggga aaggaattgc cgggctatgt
     7861 gcaacgggaa tttgaagaat ttctccaaag cccgcgacgc agcgccggca ggcagagcaa
     7921 gtagagggca gcgcctgcaa tccatgccca cccgttccac gttgttatag aagccgcata
     7981 gatcgccgtg aagaggaggg gtccgacgat cgaggtcagg ctggtgagcg ccgccagtga
     8041 gccttgcagc tgcccctgac gttcctcatc cacctgcctg gacaacattg cttgcagcgc
     8101 cggcattccg atgccacccg aagcaagcag gaccatgatc gggaacgcca tccatccccg
     8161 tgtcgcgaag gcaagcagga tgtagcctgt gccgtcggca atcattccga gcatgagtgc
     8221 ccgcctttcg ccgagccggg cggctacagg gccggtgatc attgcctggg cgagtgaatg
     8281 cagaatgcca aatgcggcaa gcgaaatgcc gatcgtggtc gcgtcccagt gaaagcgatc
     8341 ctcgccgaaa atgacccaaa gcgcggccgg cacctgtccg acaagttgca tgatgaagaa
     8401 gaccg
//
