LOCUS       NZ_CP010381             4921 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei subsp. steigerwaltii strain 34998 plasmid
            p34998-4.921kb, complete sequence.
ACCESSION   NZ_CP010381
VERSION     NZ_CP010381.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03067417
            Assembly: GCF_000807425.2
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei subsp. steigerwaltii
  ORGANISM  Enterobacter hormaechei subsp. steigerwaltii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Chavda,K.D., Chen,L., Fouts,D.E., Sutton,G., Brinkac,L.,
            Jenkins,S.G., Bonomo,R.A., Adams,M.D. and Kreiswirth,B.N.
  TITLE     Comprehensive Genome Analysis of Carbapenemase-Producing
            Enterobacter spp.: New Insights into Phylogeny, Population
            Structure, and Resistance Mechanisms
  JOURNAL   mBio 7 (6), e02093-16 (2016)
   PUBMED   27965456
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   McCorrison,J., Sanka,R., Adams,M., Brinkac,L., Sutton,G.,
            Kreiswirth,B. and Chen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2015) JCVI, 9704 Medical Center Drive, Rockville,
            MD 20850, USA
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 4921)
  AUTHORS   McCorrison,J., Sanka,R., Adams,M., Brinkac,L., Sutton,G.,
            Kreiswirth,B. and Chen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2014) JCVI, 9704 Medical Center Drive, Rockville,
            MD 20850, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Assembly Name :: HGAP assembly of Enterobacter cloacae 34998
            Expected Final Version            :: Yes
            Genome Coverage                   :: 8.58x
            Sequencing Technology             :: Pacbio RS
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000807425.2-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 01:51:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,201
            CDSs (total)                      :: 5,086
            Genes (coding)                    :: 4,983
            CDSs (with protein)               :: 4,983
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 30 of 103
            Pseudo Genes (incomplete)         :: 77 of 103
            Pseudo Genes (internal stop)      :: 14 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            Pseudo Genes (short protein)      :: 1 of 103
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP010381.1.
            The organism was changed from 'Enterobacter cloacae' to
            'Enterobacter hormaechei subsp. steigerwaltii' in June 2016.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Enterobacter hormaechei subsp. steigerwaltii"
                     /mol_type="genomic DNA"
                     /strain="34998"
                     /isolation_source="Bodily fluid"
                     /host="Homo sapiens"
                     /sub_species="steigerwaltii"
                     /db_xref="taxon:299766"
                     /plasmid="p34998-4.921kb"
                     /geo_loc_name="USA"
                     /collection_date="2011"
                     /collected_by="Barry Kreiswirth"
     gene            join(4608..4921,1..178)
                     /locus_tag="LI65_RS27600"
     CDS             join(4608..4921,1..178)
                     /locus_tag="LI65_RS27600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_047720453.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            185..400
                     /locus_tag="LI65_RS27605"
     CDS             185..400
                     /locus_tag="LI65_RS27605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021243037.1"
                     /translation="MTELEKQLLSVLEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1314..2600
                     /locus_tag="LI65_RS00010"
                     /old_locus_tag="LI65_00010"
     CDS             1314..2600
                     /locus_tag="LI65_RS00010"
                     /old_locus_tag="LI65_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437803.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_306170312.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDPLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTREGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            3607..3930
                     /locus_tag="LI65_RS00025"
                     /old_locus_tag="LI65_00030"
     CDS             3607..3930
                     /locus_tag="LI65_RS00025"
                     /old_locus_tag="LI65_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_021243036.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          3322..3494
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.851"
                     /coverage="100.0"
                     /evalue="1.98e-82"
     Typing          complement(2851..3397)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.727"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          2..498
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.391"
                     /coverage="100.0"
                     /evalue="4.95e-81"
     Typing          3921..4921
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.701"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caattacacg catcttcgtc agcaggaaga taccctggcg aaaatgaccg cccggacgtg
       61 gggagtgcgc tatcaggaga gcagcgacgg ccggaggttt cttatcctgc cgccggggat
      121 gcaagccgaa gccatcccct acgacgggac gacatggatc cgcctgaaac aggagtagcg
      181 gaaaatgaca gagctggaaa agcagttgct gagcgtatta gagcagctac agcaggacta
      241 ctcgcaaagg ctggacgaat gggagagcgc cttcgcggaa tggcggagca tgtgtggtct
      301 tatgcaacgg gagaacgcgg tgctgaacga gcgcgtgtcg gacttgagca cgcaggtgct
      361 gagtttaagc gagcagctgc gccgcttgtc cgggaactga atgccatcga ggagaaggct
      421 gcactggagt atcagcgtca gaacgagctg gagctgacgc tccgacagaa gcagaagagt
      481 ttcgatggtc cgaagatgag gtgacagccc gcacgaagaa gggctcatcg cgttcctgct
      541 aaataggctg actaccggaa tggtagtgac ttatcccgga ggaagtgatg aaaattctca
      601 acgttattga agccctgctg aactgttttt acctggtgat ggagctgtgt gagttgccgg
      661 agaggcattt gccggttttt aaggcgttcg cgctgatttt agtgtgactt tacgctgcat
      721 gcatctggct gcatgactat gcatcagtgt aatatccagc ggctgcgttt ttggtgtttt
      781 ttcgtcatgt ctgtcgccac tttctcgcgt ctggcaccgg aaacgcagcc aaccccgttt
      841 ctgcggtgcg ggcaaggggt gttatgttaa aagccgaggt ctggcggata cggaaaaaat
      901 tatgcccgcg gcaatccggc cgatacttaa attatccttc acaaaggatg tgctgcgatc
      961 caggcagttg agtgttttcc ggctcccgga tgggagccat ttttttgcct tctgatgcct
     1021 gttaatgcat gcggagctgc gctggttctt ccaagattga tagacgccag cttctctgct
     1081 ggagccggcg gatttgccgt aaatatgaac attttcttat tgttcagccc gctaaagatg
     1141 gcgggttttt tacgcgtgaa atccggagcc ggacatcatg ccggcatggc tgaatagcgg
     1201 gctttttccc gccctgtcgc agtaatatat aacgctacat aaggagtagc cgataaccat
     1261 ggcagtactt acattatccg ggcaatctga ttcatatgtt ttcacttaaa aatatgttct
     1321 ccctgaacct ccagcgatca gccatttcgt atgacgcaat ttgtcagaat aagcctgtca
     1381 ttgagttcag tccggagggg gtgataaata aagccagccc gctttttctc tccactatgg
     1441 ggtacagggc tgatgaaata atcggtcacc accatcgtat attctgcccg ccctcgctgg
     1501 taagttcacc ggaatacagc cagttctggc agcgccttgc caggggagaa agctttagtg
     1561 gcaagtacct gcggctggca aagggaaatc gttccgtatg gcttgaagcc agttatatcc
     1621 ccgtttctga caggcgcggc cgtgttatca gagtcattaa aattgctgcc gatatttctg
     1681 agcgcgtgca ttctgctctt gagcaggaag cagttgtaaa tgccataaac cgttctatgg
     1741 ccatcatcac cttcaatcct gaagggatcg tgcttgaagc aaatgaaaat ttcgtcaatg
     1801 ccactggcta taagcgggat gaaatcatag gtaagcatca tcgtctgttc tgtgccgaga
     1861 cgctttacaa aagtgatgaa taccgacatt tctgggagag tctgaatcaa ggtgagtttt
     1921 tttctggtct gtttccacgc cttaaccgtc agggagaccc tctgtggttc cgtgcaactt
     1981 ataaccctgt ctttaacagt gatgggcagc tttataaaat tgtgaaattc gctacagatg
     2041 ttacggctga tgtcctgcga aaccagagag agcaagaggc tgctgtgcat gcatgggata
     2101 tggccgtgca gacccgagaa agtgcgcaga acggtgccaa tgttattgaa aatagcatcc
     2161 ttatgattga cagaattgcg caggggatgg gggctgtctc caccgatatc tcacggctca
     2221 acaatcagtc tgaaagtatt gacgatatgg tggaaaccat ccggaagttt gccatgcaga
     2281 caagacttat tgcactgaat gccgcgattg aagcagcaag ggctggcgca tccggaagaa
     2341 gttttgcggt tgttgccgca gaagtgcgta atcttgcggc gagcgtcagt agcgcgactg
     2401 aagaaattga gcaagtcgtg gccagcaata gtcaactggc caaggatgtt ctctgtggca
     2461 ttgaaaacag cctgatgaac actcgggaag gggttacact catgcgcgaa gcgggtgaag
     2521 ttatgaccag cattcagagg aatgctgcag gggttgagac tgcggttaag gatgtcgcca
     2581 tttcagttaa agccggatag gccatggtgc atatccccat atctgtcgaa catcacagat
     2641 gatcttcttg agatcctttc cgttgcgtcg taatctcttg ctctgaaaac gaaaaaacca
     2701 cccggcaagg tggctttttc gaaggttcgc aagagttgat gctcttttga accgcggtaa
     2761 ctggcttgga ggagcacagt caccaaaact gtcctttcag tgtagcctta tcgcacccga
     2821 aacttcaaga ctctcctcct ctaaacctgt taccagtggc tgctgccagt ggcgctttgt
     2881 cgtgtttttc cggattggac tcaagttgat agttaccgga taaggcgcag cggtcggact
     2941 gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgccttcccg gaactgagtg
     3001 tcaggcgtgg aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag
     3061 gcaggaacag gagagcgcac gagggagcca ccagggggaa acgcctggta tctttatagt
     3121 cctgtcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg
     3181 cggagcctat ggaaaaacgg cttcgccgtg gccttatcgc ttccctgtta agcttctttc
     3241 tggcatctcc caggaaattt ccgccccgcc tgtaagccgg taccgctcgc cgcagccgaa
     3301 cgaccgagcg cagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct
     3361 gctgacgctc cgctgctgca tttttctcct gccacatgaa gcacttcact gatattcgca
     3421 tccgtgccaa tatagtaagc cagtatacac tccgctagcg ctacgtgact ggttcagggc
     3481 tgcgccccga aacccgccaa aagcctgacg cgcctgcggc ttgtccaacc ccgcgccgac
     3541 cgggaaaggc tttcccgccc gtcccggcgt tatcaggagg ccggtttgtc agagaaacgg
     3601 aacaagatgc tcaccatgtg ggtgacggag gacgagcacc ggcggctgct tgagcgctgt
     3661 gacggcaggc agctggcggc gtggatgcgg cagacctgcc tggacgaaaa acctgcgcgc
     3721 accggaaagc tcccctcaat ctcgccggcg ctgctgcgcc agcttgccgg catggggaat
     3781 aacctgaacc agatagcccg ccgtgttaac gccggcggcg gcaccggcca tgacagggtg
     3841 cagattgtgg cggcgctgat ggccatcgat gccgggcttg agcggctgcg gcacgccgtt
     3901 cttgagaaag gaacggacga tgatcgttaa atttcatcca cgtggccgcg gcggtggcgc
     3961 cgggccggtg gactatctgc tgggtaaaga ccggcagcgt gaaggcgcca gcgtcctgca
     4021 ggggaagccg gaagaagtcc gggagctgat tgacgcgtcg ccttacgtga agaaatacac
     4081 ctccggcgtc ctgtcgtttg cggaggccga tctgcctccc ggccagcggg aaaaactgat
     4141 ggccagcttc gagcgggttc tgatgcccgg acttgataaa gatcagtaca gcattctgtg
     4201 ggtggagcac gaggacaagg ggcgtctgga gctgaacttt ctgatcccca ataccgagct
     4261 gctgaccggc aggcggctcc agccgtacta cgaccgcgcc gaccggccgc gtatcgatgc
     4321 ctggcagacc atcgtgaacg gcagactggg gctgcacgat ccgaacgcgc cggaaaaccg
     4381 ccgggcgctg gtcacgccgg ccggactgcc gaaaacgaag caggaagccg cagaagccat
     4441 cacccgtgga ctgctgagct tagcctcgtc cggggagctg aaaagccgtc aggacgtcac
     4501 tgaggctctt gaaggtgcag gttttgaggt ggtgcgtaca acgaaaagca gtatcagcat
     4561 tgccgaccct gacggggggc gaaacatccg actgaaggga gccatctatg agcagtcttt
     4621 taacgctggc gaaggactta gagcagcaat cgaaagcgca gcagaagagt accggcgaga
     4681 tgctgaaagc cgcattcagc gagcacgagc agtctgtcag agcggaactg aacgcaagcg
     4741 cgaggagaat cagcgacgcc atcagcgccc acgagcagag tatgagcgag gcgatggaga
     4801 aaaaccggcg gagcgtgttg ctgacggccg ggaggacatg gctcaccatc ctgatggtgt
     4861 cagcgctgct gatcgccacg agcgggagca ttctgtggtg gcaggggcag cagataaccg
     4921 a
//
