LOCUS       NZ_CP010375             5007 bp    DNA     circular CON 03-APR-2024
DEFINITION  Enterobacter hormaechei subsp. steigerwaltii strain 34977 plasmid
            p34977-5.006kb, complete sequence.
ACCESSION   NZ_CP010375
VERSION     NZ_CP010375.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03067414
            Assembly: GCF_000814125.3
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei subsp. steigerwaltii
  ORGANISM  Enterobacter hormaechei subsp. steigerwaltii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Chavda,K.D., Chen,L., Fouts,D.E., Sutton,G., Brinkac,L.,
            Jenkins,S.G., Bonomo,R.A., Adams,M.D. and Kreiswirth,B.N.
  TITLE     Comprehensive Genome Analysis of Carbapenemase-Producing
            Enterobacter spp.: New Insights into Phylogeny, Population
            Structure, and Resistance Mechanisms
  JOURNAL   mBio 7 (6), e02093-16 (2016)
   PUBMED   27965456
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   McCorrison,J., Sanka,R., Adams,M., Brinkac,L., Sutton,G.,
            Kreiswirth,B. and Chen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2014) JCVI, 9704 Medical Center Drive, Rockville,
            MD 20850, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Assembly Name :: HGAP assembly of Enterobacter cloacae 34977
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.37x
            Sequencing Technology             :: Pacbio RS
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000814125.3-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 01:46:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,043
            CDSs (total)                      :: 4,929
            Genes (coding)                    :: 4,853
            CDSs (with protein)               :: 4,853
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 27 of 76
            Pseudo Genes (incomplete)         :: 53 of 76
            Pseudo Genes (internal stop)      :: 12 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            Pseudo Genes (short protein)      :: 1 of 76
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP010375.2.
            On Aug 20, 2015 this sequence version replaced NZ_CP010375.1.
            The organism was changed from 'Enterobacter cloacae' to
            'Enterobacter hormaechei subsp. steigerwaltii' in June 2016.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Enterobacter hormaechei subsp. steigerwaltii"
                     /mol_type="genomic DNA"
                     /strain="34977"
                     /isolation_source="Bodily fluid"
                     /host="Homo sapiens"
                     /sub_species="steigerwaltii"
                     /db_xref="taxon:299766"
                     /plasmid="p34977-5.006kb"
                     /geo_loc_name="USA"
                     /collection_date="2009"
                     /collected_by="Barry Kreiswirth"
     gene            complement(join(4965..5007,1..545))
                     /locus_tag="LI62_RS00245"
                     /old_locus_tag="LI62_24990"
     CDS             complement(join(4965..5007,1..545))
                     /locus_tag="LI62_RS00245"
                     /old_locus_tag="LI62_24990"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045336834.1"
                     /translation="MIRKIFFASAIVIAISGCTAMDKLAGMGVVSQQTSTFDNSTVIEV
                     SPNSLYDPNSTWGTQLQLGAIWSSAYPEEVGLILSYNSNVSSGNAAYLGLSGIDINIDG
                     KISSYKAQQQTNLSSSAYNSVSKTIYTSSKNTVVIPYKLLESMVSAKNCRIRIHTSQGN
                     QDIDFSAQSIPGGKATAIVSIKEFMVKVESFK"
                     /name="hypothetical protein"
     gene            complement(839..985)
                     /locus_tag="LI62_RS26890"
                     /old_locus_tag="LI62_00260"
     CDS             complement(839..985)
                     /locus_tag="LI62_RS26890"
                     /old_locus_tag="LI62_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040117003.1"
                     /translation="MHEPPVQSDCCALSGNYQLESNPERHDLSPLAAAIGKRMYKRLLT
                     QSS"
                     /name="hypothetical protein"
     gene            1627..1950
                     /locus_tag="LI62_RS00255"
                     /old_locus_tag="LI62_00265"
     CDS             1627..1950
                     /locus_tag="LI62_RS00255"
                     /old_locus_tag="LI62_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_172751439.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPTLLRQLAGMGNNLNQIARQVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2159..>2593
                     /locus_tag="LI62_RS26690"
                     /pseudo=""
     CDS             2159..>2593
                     /locus_tag="LI62_RS26690"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2628..3113
                     /locus_tag="LI62_RS26035"
     CDS             2628..3113
                     /locus_tag="LI62_RS26035"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_137984564.1"
                     /translation="MNSLLTLAKNLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELNESE
                     KRISAAILDHDQKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLTASGAGILWWQGQLIA
                     DNYTTIREQTRTQAMLSERNNGVQLTTCGEKRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            3117..3332
                     /locus_tag="LI62_RS26040"
     CDS             3117..3332
                     /locus_tag="LI62_RS26040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_137984565.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4242..4511
                     /locus_tag="LI62_RS00265"
                     /old_locus_tag="LI62_00275"
     CDS             4242..4511
                     /locus_tag="LI62_RS00265"
                     /old_locus_tag="LI62_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040078821.1"
                     /translation="MNQTMSQKLARDSLGNAQTFEGGVYVTKNGVAELFIQTAAERDAE
                     VREMREERNINALFKLATLAKKEIAEGKGMTPEEVLGRLRAARK"
                     /name="hypothetical protein"
     gene            4526..4864
                     /locus_tag="LI62_RS00270"
                     /old_locus_tag="LI62_00280"
     CDS             4526..4864
                     /locus_tag="LI62_RS00270"
                     /old_locus_tag="LI62_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040117006.1"
                     /translation="MSQQYTVSIAPMAYQCLADIESYKSGYIGSLKAGDLVDNLLQESV
                     ACIIQDPERYHYNFHLQDRGMLLRERLDVESEYRVLYDFDGTNIEILLFVSMKQDFEKQ
                     LYRYNLLN"
                     /name="hypothetical protein"
     Typing          1340..1531
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.333"
                     /coverage="89.0"
                     /evalue="1.56e-74"
     Typing          complement(877..1357)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.866"
                     /coverage="92.0"
                     /evalue="2.75e-165"
     Typing          1941..3436
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.581"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acgatcgctg tcgctttacc tccagggatg ctttgcgccg agaagtcgat gtcttgattt
       61 ccttggcttg tgtgtatgcg aatgcggcag tttttagctg ataccatgct ctctagcaat
      121 ttatagggga taacaacagt gttcttgcta gaggtataga tcgttttaga tacggagtta
      181 tatgcgctgc tgcttagatt agtttgctgc tgtgctttat agctggatat tttgccatca
      241 atatttatat caatccccga caaacctaag tatgcggcat ttccgctaga tacattcgag
      301 ttgtaggaaa gaattagccc aacctcttct ggataggcac tagaccatat cgcgcccaac
      361 tgaagttgtg ttccccaagt gctgttagga tcgtatagcg aatttggaga tacctctatg
      421 acggtcgagt tatcaaaggt tgatgtttgc tgagaaacaa cacccattcc tgccaattta
      481 tccattgctg tacagccaga gatggctatt acgattgcag aagcaaaaaa aatctttctt
      541 atcattttgt tgaccttttt ggtctgtttg ccagagagtg aaatagcatt gtatttctga
      601 aactatcttg aagtcttata gctttttaaa gagtgttttg aacagagagt tgaaggatct
      661 tcttgagatc ctcgggtggg agtcgtaatc tcttgaccca gaaacgaaaa aaccacctgg
      721 ggaggtggtt tttcgatggt tcagtaagtt ggggaacttc tgaaccgtgg taacagggtg
      781 tacaagacca ctgccaccaa attcgtcctt ccagtttagc cgtagttggg ctgatacttc
      841 aagaactctg cgttagtaat ctcttgtaca tcctcttacc aatggctgct gccagtggcg
      901 ataagtcgtg tctttccggg ttggactcaa gttgatagtt accggataag gcgcagcagt
      961 cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc tacccggaac
     1021 tgagtgtcag gcgtggaatg agacaaacgc ggccataaca gcggaatgac accggtaaac
     1081 cgaaaggcag gaacaggaga gcgcgcgagg gagctaccag ggggaaacgc ctgggatctt
     1141 tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agattttatg atactcgtca
     1201 ggggggcgga gcctatggaa aaacggcaac gccgcatcct ttttcttgag taccattaga
     1261 ttgcgttcaa tctaatgctc tggcctgtgt gagccgataa cgcccgccgc agtctcacaa
     1321 caggagcgta gcgaccgagt gagcgaggag gcgtcatttc acctttaccc gcatgttgca
     1381 cctacgcccg gcagccgcat ttttcccttg ctacatgaag cactttcata aatcaccact
     1441 ccgccgtaac ataataagcc agtatacact ccgctagcgc tacgtgactg gttcagggct
     1501 gcgccccgaa acccgctaaa accactgccg cgcctgcggc ttgcccaaca ccgcgccgct
     1561 cgggaaaaga tttcccgttc gtcccggcgt tatcaggagg ccggattggc aggcaaacgc
     1621 agcaaaatgc tcacgatgtg ggttactgag gatgagcacc gccgacttct ggagcgttgc
     1681 gacggtaaac agctcgcggc ctggatgcgt cagacgtgtt tggacgagaa gcctgcccgt
     1741 gccggcaaac ttccgtcgat ctcgccgacg ttgcttcgtc agctcgccgg catgggtaac
     1801 aacctcaacc agattgcccg gcaggttaat gccggtggcg gtaccgggca cgaccgcgtg
     1861 cagattgtcg ccgcgctgat ggccatcgat gccggactcg agcggctgag gcacgccgtt
     1921 ctggaaaagg gggcagacga tgatcgttaa atttcatccc agaggccgcg gcggcggcgc
     1981 cggtccggtg gattatctgc tgggaaaaga ccgccagcgc gacggtgcca gcgttctgca
     2041 gggtaagccg gaggaagtcc gggagcttat tgacgcctca ccctacgcca aaaagtacac
     2101 ctccggcgtc ctgtcctttg ccgaaaagga tttaccgccc ggccagcgcg aaaagctgat
     2161 ggcgggcttt gagcaagttc tgatgcccgg actcgataaa gaccagtaca gcgtgctgtg
     2221 ggttgagcac cgggataagg ggcgactgga gctgaatttt ctgatcccga acacggagct
     2281 gctgaccggc aggcgtctcc agccgtatta cgaccgcgcc gaccggccgc gcatcgacgc
     2341 ctggcagact atcgtgaacg gcaggctggg actgcacgac ccgaacgcgc cggagaaccg
     2401 gcgggcgctg gttacgccgt ccgcgctgcc ggaagcgaag ctggaggcag cccaggcgat
     2461 tacgcgcggc ctgcttactc ttgccgcgtc cggagagctg aaaacgcgcc aggacgttac
     2521 tgaggcgctg aaaagcgcgg gttttgatgt ggtccgcacc acgaaaagca gcatcagcat
     2581 cgctgacccg gacggggggc gaaacatccg actgaaggga gccatctatg aacagtcttt
     2641 taacgctggc gaagaactta gagcagaaat cgaaagcgca gcagcagact accggcgaga
     2701 tgctgaaagc cgcattcagc gagcacgaga agtctgtcag agcggaactg aacgaaagcg
     2761 agaagagaat cagcgccgcc atcctcgacc acgaccagaa actgtcctcg gccatgagcc
     2821 agcgcacgaa aggcatggtg cgcatggtca gccagacgtg gctgaccatc gtcctggtgt
     2881 ccgcgctgct gacagcctca ggcgcgggca ttctgtggtg gcagggacag ctgatagccg
     2941 acaactacac gaccatccgg gagcagacgc gcacgcaggc catgctgtca gagaggaaca
     3001 acggcgtgca gctcacgacc tgcggggaga agagacgccg ctgcgtgagg gtgaacccgg
     3061 aagcgggacg attcggagag gattcgagct ggatgatact ggcggggaaa tagcacatga
     3121 cagagctgga aaaacagttg ctgagcgcat tagagcagct acagcaggac tactcgaaaa
     3181 ggctggacga gtgggagaac gccttcgcgg aatggcggac gatgtctggt cttatgcaac
     3241 gggagaacgc ggcgctgagc gagcgcgtca cgggcttgag caggcaggtg cagagtttga
     3301 gcgagcagct gcgccggttg tcgaaaggct gaacgggata gagacgcacc gggagcaggt
     3361 gcgggcggcg cagcatcaga aagaactgga gctggagcga tcgcagcggc agcggacgta
     3421 tgacgggcca tcgctgtaac aaaccggata aagcagagct ttgatccggc gcttgcaccg
     3481 aaagccttag ctgctcactt tgattttccg gaggcgtcat taccggattt tgggcgtgag
     3541 ggtgcattac ctctgcctcc tccagactta tttcctgtcg tggatggcca gttaccaccg
     3601 ttgtcagggt tgcttgacga tgggcctttt gaagtgtttt tagacatgtg aatctccttt
     3661 tctctttgac ggatacagga cagacagcct gcattcagta aactatctgt tgcgccggat
     3721 ggcttgtcca gcgcggtaaa ctgcacgccc gttcaccgtt accggaaatg accgtttcct
     3781 gcgaaaacgc ggctttagtg ccagcatccg cccggaactg ggtgccattc actcccgtgc
     3841 atagctatgc acccgcgcca gaatcatcag gggcgttttt tcacgatatg gccggggaaa
     3901 ggtgccggaa acggcccgct cgcgtcagga tcgcgctgag ggcccctgcg ggggtgcggc
     3961 caagagatgt tatggtaaat gcttctgaat tagtctaatt ttcccttcct gcaccttgtt
     4021 gtgtaaaaat atgtactcaa tttatccacc aactgataaa ctctattcaa agcacggaca
     4081 ccggccacca tgtgccatag cgagaatttg tactggccta atcaatcaac actgaaacct
     4141 gaaacctgaa aactgaaacc acaaaccgga aactatgacc cttgaatcgt gcagcgccgt
     4201 tctcgccgga agcgccccta tatgatgaga aaaaaatgcc tatgaaccag accatgtccc
     4261 agaaactcgc aagagacagt ctcggaaatg cacaaacctt tgaaggaggt gtttacgtca
     4321 ctaaaaacgg agtagctgag ttattcatcc aaaccgcagc agaacgtgat gctgaagttc
     4381 gtgagatgag agaagaacgc aatatcaacg ccctttttaa gctggcaacg ttggctaaaa
     4441 aggaaatcgc agaaggcaaa ggcatgaccc cagaagaggt cttggggagg ttgcgtgccg
     4501 ctagaaaata acagagaggt gtttagtgtc ccagcaatac acggtttcaa tcgcgccgat
     4561 ggcatatcaa tgtttagctg atattgagtc ttacaagagt ggatacattg ggtcactcaa
     4621 ggccggtgat ctggttgata acttacttca ggaatccgtt gcctgcatta tccaagaccc
     4681 tgaacgttat cactacaatt tccacttaca ggatcgaggt atgcttttac gtgagcggct
     4741 cgatgttgag agtgaataca gggtgcttta cgattttgac ggaacgaaca tcgaaatact
     4801 gttgtttgtg agcatgaagc aggattttga aaagcagctg tatcgctata atttgctgaa
     4861 ttaacactaa acacccggca tcgtcgggtt ttttcgtgca cacaaaaatt tccacctatg
     4921 cctattaaca ctaagttagt agatataggt gggcttacgt gtccttattt aaaactttcg
     4981 actttgacca tgaactcttt aatggaa
//
