LOCUS       NZ_CP010357            13330 bp    DNA     circular CON 25-DEC-2023
DEFINITION  Acinetobacter johnsonii XBB1 plasmid pXBB1-6, complete sequence.
ACCESSION   NZ_CP010357
VERSION     NZ_CP010357.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03268929
            Assembly: GCF_001484935.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter johnsonii XBB1
  ORGANISM  Acinetobacter johnsonii XBB1
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 13330)
  AUTHORS   Zong,Z.
  TITLE     The complete genome of Acinetobacter johnsonnii strain XBB1 carrying
            blaNDM-1 and blaOXA-58 from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13330)
  AUTHORS   Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2014) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.6; Sspace v. 2.0
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina; 454
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/25/2023 04:54:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,995
            CDSs (total)                      :: 3,887
            Genes (coding)                    :: 3,684
            CDSs (with protein)               :: 3,684
            Genes (RNA)                       :: 108
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 203
            CDSs (without protein)            :: 203
            Pseudo Genes (ambiguous residues) :: 0 of 203
            Pseudo Genes (frameshifted)       :: 103 of 203
            Pseudo Genes (incomplete)         :: 102 of 203
            Pseudo Genes (internal stop)      :: 46 of 203
            Pseudo Genes (multiple problems)  :: 41 of 203
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP010357.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13330
                     /organism="Acinetobacter johnsonii XBB1"
                     /mol_type="genomic DNA"
                     /strain="XBB1"
                     /db_xref="taxon:1242245"
                     /plasmid="pXBB1-6"
     gene            72..1162
                     /locus_tag="EHS42_RS19585"
                     /old_locus_tag="RZ95_RS20570"
     CDS             join(72..612,612..1162)
                     /locus_tag="EHS42_RS19585"
                     /old_locus_tag="RZ95_RS20570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612069.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISAba1 family transposase"
                     /protein_id="WP_085947913.1"
                     /translation="MTHLNELYLILNKSLKWNKSHLKCFALIMLVIILKQTCNLSSASK
                     ALPIKCLPQSFYRRMQRFFAGQYFDYRQISQLIFNMFSFDQVQLTLDRTNWKWGKRNIN
                     ILMLAIVYRGIAIPILWTLLNKRGNSDTKERIALIQRFIAIFGKDRIVNVFADREFIGE
                     QWFTWLIEQDINFCIRVKKNFIVTNHLGKNHKISDLFRHLKVGQIECRKRRILVGRVKL
                     YISALQLENGELLLVVSPQFNANAIQDYALRWEIETLFSCLKGRGFNLENTRLTDPRRV
                     KKLIAVLAISFCWCYLTGEWQHDQKKAIKIKKHGRLSMSLFRYGLDYVQMAIQRLIGFG
                     KKEEFKEILAILRRQNPDRIRVL"
                     /name="IS4-like element ISAba1 family transposase"
     gene            1191..3254
                     /locus_tag="EHS42_RS19590"
                     /old_locus_tag="RZ95_19425"
                     /old_locus_tag="RZ95_RS19380"
     CDS             1191..3254
                     /locus_tag="EHS42_RS19590"
                     /old_locus_tag="RZ95_19425"
                     /old_locus_tag="RZ95_RS19380"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_212839219.1"
                     /translation="MKSRQEPNYVAKLHAQAKKIMDAESLQDLTKKQFCKFIDDSISRL
                     AAHPLSGIKTRADIVQEIKKHKDVKSVKQGRDYLTVELTGGKKHRLKGFLYHDNFSREL
                     LSEHIKAERERRNSPSELAAAINQTGKLREEYRTKRETYHRRHHSFTPSTANDNSPRIA
                     STLDFDSDKESITPSNTNAHNELYGANTSITSTAMQYRYIEHRNRHNQHQFVFNINRGV
                     TGAHQQPSQKPQDRSKELTEQYPSPSPTSNSKPKRTNPERHPDLNHGKIEYSESNLPPI
                     RNYSFWSADSFNEFISQLSDQFKSKNDQSTTRSDSTELTNNFKSKQQHELIAEENNADR
                     NRPFFERAKRLIDSTKQLVSRAKQRIESTSEFIKKHLEQIQRSRAELTKQNQQLEYRER
                     NPAGLDPGYEIRTFKTRTGQLFGAITASISKQCADAITDAINESIKSTGFKQRCQRFSE
                     DRIETHSFEYQHKADRSQERTLENLASEGTRQLLQRSKDAEQANRHARGDSGNISQINQ
                     QLRGLEKTVAKIRLKPKPTTDFYLNLLRNDNYYPDYVLYHKELCDEQQQAFNNQDTLLL
                     IDYTKKKAENLETYMSRASRNLNKSDYEKIEQIIKNDQRMLKYLQCEKILERQDTRFEQ
                     HRAAYQSCLKRFENIKLDIEQIKNSSSQHNVSQNTYEPEYKTKPKNDLDFNF"
                     /name="mobilization protein"
     gene            4193..5128
                     /gene="repM"
                     /locus_tag="EHS42_RS19595"
                     /old_locus_tag="RZ95_19435"
                     /old_locus_tag="RZ95_RS19385"
     CDS             4193..5128
                     /gene="repM"
                     /locus_tag="EHS42_RS19595"
                     /old_locus_tag="RZ95_19435"
                     /old_locus_tag="RZ95_RS19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_058952649.1"
                     /translation="MKKEIVKDNTLVQASYTLDIVEQRLILLAIAEARETGHGITENSL
                     LAIHASSYINTFNVEKHTAYTVLRDASKSLFKRYVTYHDINPKTGKNRSFHCRWVDKIG
                     YEKKSGIIYLRFTQEIVPLITRLEENFTKYDLQQVSKLTSAYAIRLYELLIQWRSTGKT
                     PVFNLSIFRQQLGVEEHQYKTMSNFKTYVLEFALEQVNKSTDISAKYQQHKNGRSISGF
                     SFSFKHKKVTNPDSENKRDPNTLDAFSKMTDAQRHLFSNKLSKLPEMSKYSQGTESYQQ
                     FAIRIAEMLQDPTRFEELHPYLQKVGFKAA"
                     /name="repM"
     gene            5207..5737
                     /locus_tag="EHS42_RS19600"
                     /old_locus_tag="RZ95_19440"
                     /old_locus_tag="RZ95_RS19390"
     CDS             5207..5737
                     /locus_tag="EHS42_RS19600"
                     /old_locus_tag="RZ95_19440"
                     /old_locus_tag="RZ95_RS19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_058952650.1"
                     /translation="MKSLSVLELSKLYSINRQTIYNQINKGILSKNSDNKIDLAEAIRV
                     FGEPIRKQDVKEPVKVDSPNSAEVLLLKQQIDMLKNQLEDAKDRESFYQNQIETMQRLL
                     EAPKPNMTTFTDQEPVQASMVKTDPEPDTAEAKHDGLTTPEKTENKRIPVPEHIEQEPE
                     KRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(5946..6797)
                     /locus_tag="EHS42_RS19605"
                     /old_locus_tag="RZ95_19445"
                     /old_locus_tag="RZ95_RS19395"
     CDS             complement(5946..6797)
                     /locus_tag="EHS42_RS19605"
                     /old_locus_tag="RZ95_19445"
                     /old_locus_tag="RZ95_RS19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="WP_002115246.1"
                     /translation="MSKIIACIDGSLATNTVCDYAAWFSDKLNSPLQLLHVIDKPKAKA
                     PQDLSGAIGLGSRETLLNELVELEERKGKIELEHGQILLREAKNYLLEKFSIDAQSFQR
                     HGSLLETIMGMEEDIRVLVMGKHGTETEHNSSKVGTHIENVVRALHKPVLITSSPFSPP
                     QSFLIAFDGSPTARICVDKIANSPLLKTLTAHVVYVGKPNSDMTSQVTWAKELLANNGF
                     DVIDSVLEGDVEKSILDYQEKNVIDMIVIGAYGHSKIRQFFIGSTTTKLLSSSTKPVLL
                     LR"
                     /name="universal stress protein"
     gene            complement(6810..8297)
                     /locus_tag="EHS42_RS19610"
                     /old_locus_tag="RZ95_19450"
                     /old_locus_tag="RZ95_RS19400"
     CDS             complement(6810..8297)
                     /locus_tag="EHS42_RS19610"
                     /old_locus_tag="RZ95_19450"
                     /old_locus_tag="RZ95_RS19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953322.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="WP_005146104.1"
                     /translation="MLSTIREQWFSNIRGDVLAGIVVALALIPEAIAFSIIAGVDPKVG
                     LYASFCIAVVISFVGGRPGMISAATGAMALLMVTLVKEHGLQYLLAATILTGFIQILAG
                     YLKLGALMRFVSKSVVIGFVNALAILIFMAQLPELTNVTWHVYAMTIGGLAIIYLFPYI
                     PVIGKLLPSPLICIVLLTLFALFIGLDVRTVGDMGQLPDTLPIFLLPDIPLNLETLAII
                     LPYSLGLAAVGLLESMMTATIVDDLTDTTSDKNRECKGQGVANVASGFLGGMAGCAMIG
                     QSIINVKSGGRTRLSTFSAGVFLLIMVVFISDWLKVIPMAALVAVMIMVSIGTFNWDSL
                     RNIRQYPFSSNIVMIATVVVVVATHNLAYGVLVGVLLSALFFANKIERYMAIQSEFNEP
                     ESTRTYTVTGQVFFSSADKFTSAFDFKEALSKVVIDVSQAHFWDVSAVAAVDKVVIKFR
                     REGTEVELIGMNEASKTLVDKFGIHDKPDALDRLNSH"
                     /name="SulP family inorganic anion transporter"
     gene            complement(9042..>9179)
                     /locus_tag="EHS42_RS19615"
                     /old_locus_tag="RZ95_RS20580"
                     /pseudo=""
     CDS             complement(9042..>9179)
                     /locus_tag="EHS42_RS19615"
                     /old_locus_tag="RZ95_RS20580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            9483..10526
                     /locus_tag="EHS42_RS19625"
                     /old_locus_tag="RZ95_19455"
                     /old_locus_tag="RZ95_RS19405"
     CDS             9483..10526
                     /locus_tag="EHS42_RS19625"
                     /old_locus_tag="RZ95_19455"
                     /old_locus_tag="RZ95_RS19405"
                     /inference="COORDINATES: protein motif:HMM:NF019371.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_005175450.1"
                     /translation="MQISDSAAFFNSISHSRINSYKNYFGSHLSSEELIACYQWNETVS
                     VTFFKVINLIEIILRNRFHLTLSNHFYSIPKEQVIAKRVPARSSFRYQNVPTLGTADSC
                     NWYETRLLEKKSLQKVLDKTHHKSGSPLRVPIFPDDLVAGLTLGFWCSLIPKNLSMPWD
                     QQLNNIFPNHRLTKSFIANSRSSPTTASNPWLNVDNVQQVSNRLELLNEFRNRIAHHEP
                     LWKLPDLYDEIPTSTEKKGTVIATKTTNKIETFQRLNLLHHRFIELLKWIDINLAQDYQ
                     SSKNYYYLKWLISNNGFNSYINRYQNFDHCLHSSILKRNLNSLLKQQESFLIYRNHGNI
                     YALLPIT"
                     /name="Abi family protein"
     gene            10595..10996
                     /locus_tag="EHS42_RS19630"
                     /old_locus_tag="RZ95_19460"
                     /old_locus_tag="RZ95_RS19410"
     CDS             10595..10996
                     /locus_tag="EHS42_RS19630"
                     /old_locus_tag="RZ95_19460"
                     /old_locus_tag="RZ95_RS19410"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_034597323.1"
                     /translation="MDHQIFQKAMQSGGRLTVKSALNPILWLCGIISIPTIAANVFYTT
                     LPIWISIIGCAPILMAMFGFMVLLFIDRDKLQSEDYQIKKISLEMSEQKGDSNPRLVGP
                     SEILTTNPELPTTLEVLEHSDEKSSKVAK"
                     /name="hypothetical protein"
     gene            10993..11283
                     /locus_tag="EHS42_RS19635"
                     /old_locus_tag="RZ95_19465"
                     /old_locus_tag="RZ95_RS19415"
     CDS             10993..11283
                     /locus_tag="EHS42_RS19635"
                     /old_locus_tag="RZ95_19465"
                     /old_locus_tag="RZ95_RS19415"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005175448.1"
                     /translation="MTKSYLLVFSDKTGTKDEVKLALNKMTKVKTWRTDMPNCFYVISD
                     NSAQELYEEFTSIRGTSGRFLFAETASNRQGLLPSDTWYLLKNKKLKSKET"
                     /name="hypothetical protein"
     gene            complement(11404..11628)
                     /locus_tag="EHS42_RS19640"
                     /old_locus_tag="RZ95_19470"
                     /old_locus_tag="RZ95_RS19420"
     CDS             complement(11404..11628)
                     /locus_tag="EHS42_RS19640"
                     /old_locus_tag="RZ95_19470"
                     /old_locus_tag="RZ95_RS19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058870605.1"
                     /translation="MPYEEFQRLIGKSGLSIKEFAALLDMNANSITNYKKNGKVPTTIA
                     VIAIVISDMKDDGLDFYPIFEKVRAYRDQ"
                     /name="hypothetical protein"
     gene            11777..12022
                     /locus_tag="EHS42_RS19645"
                     /old_locus_tag="RZ95_19475"
                     /old_locus_tag="RZ95_RS19425"
     CDS             11777..12022
                     /locus_tag="EHS42_RS19645"
                     /old_locus_tag="RZ95_19475"
                     /old_locus_tag="RZ95_RS19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005401978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058952651.1"
                     /translation="MNHFVQPKLLISSQAKKIGLALINDLELCITARLAFYLDVNPSYD
                     GHEATFIWIEYFLQDHPQVEFSEIKEAFLKLIPECC"
                     /name="hypothetical protein"
     gene            12377..12868
                     /gene="mobC"
                     /locus_tag="EHS42_RS19650"
                     /old_locus_tag="RZ95_19480"
                     /old_locus_tag="RZ95_RS19430"
     CDS             12377..12868
                     /gene="mobC"
                     /locus_tag="EHS42_RS19650"
                     /old_locus_tag="RZ95_19480"
                     /old_locus_tag="RZ95_RS19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_058952652.1"
                     /translation="MLRTKTKSFRLTELQLKQLEIHLKKENSEFSDFLHSLIQREIMQD
                     TVTVQQIQDEIKPLRTKVKTVVEVVKRYQKTDPDLLLELAKIGNNINQIARALNIIKNA
                     NPQEQRKLDILKIFLVLKAIQTELEQVFPALPKISRQSPERLKKQLSSLQLAEQDESAY
                     "
                     /name="mobC"
     gene            12858..>13328
                     /locus_tag="EHS42_RS19655"
                     /old_locus_tag="RZ95_19485"
                     /old_locus_tag="RZ95_RS19365"
                     /pseudo=""
     CDS             12858..>13328
                     /locus_tag="EHS42_RS19655"
                     /old_locus_tag="RZ95_19485"
                     /old_locus_tag="RZ95_RS19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155982.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          4194..5128
                     /name="rep_cluster_1241"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctctgtacac gacaaaaata gataactcat tgaaataatg tcataataat tgttttctaa
       61 cgacgaatac tatgacacat ctcaatgagt tatatcttat cttaaacaaa tctctaaaat
      121 ggaacaagtc acatttaaag tgctttgcgc tcatcatgct tgtgattatt ttaaagcaaa
      181 catgtaatct ttcttctgca tctaaagcct tgcccatcaa gtgcttacca caatcatttt
      241 atcgacgtat gcagcgcttc tttgcaggtc agtattttga ttatcgtcaa atttctcagt
      301 tgattttcaa tatgttttca ttcgaccaag tgcaactgac tttagataga accaattgga
      361 aatggggaaa acgaaatatt aatatcctga tgctcgcaat cgtttatcgt ggaatagcga
      421 tacctatcct ttggacattg cttaataaac gtggaaattc agatacgaaa gagcgcattg
      481 ctttgattca acgctttata gccatttttg gtaaagaccg tattgtgaat gtgttcgcag
      541 acagagagtt tatcggtgag cagtggttta catggttaat tgaacaagac atcaacttct
      601 gcattcgtgt taaaaaaact tcattgtcac caatcattta ggaaagaatc ataaaattag
      661 tgatttattt cgccatctta aagttggtca aattgaatgt cgtaaacgac ggattttggt
      721 tggtcgggtg aaactatata taagtgcact acagttagaa aatggagagc ttttactcgt
      781 cgtttctcct cagtttaatg ccaatgctat tcaggattat gcattacgct gggaaattga
      841 aaccttattc agttgtctca aaggacgcgg gtttaatctt gaaaatacgc gcttgacaga
      901 ccctagacga gtgaaaaaat tgattgcggt gttagctata agcttctgtt ggtgttactt
      961 aacgggtgaa tggcaacatg atcaaaaaaa agcgataaaa ataaagaagc atggacgact
     1021 ctcaatgagt ttatttcgct atggtttaga ctatgttcaa atggcgattc agcgtttaat
     1081 tggttttggg aaaaaagaag agtttaagga aattttggca attttaagaa ggcagaatcc
     1141 tgataggata agggttctgt gaaatttgtc gtgtacagag tttgctttta atgaagtcca
     1201 gacaagagcc gaattatgtt gcaaaactcc atgctcaagc aaaaaagatt atggatgctg
     1261 aatctcttca agatcttaca aaaaaacagt tttgcaaatt tatagacgac tctatctcac
     1321 gccttgcggc tcacccactc agcggcatta agacgcgtgc tgacatcgtt caagaaatta
     1381 aaaaacataa agacgtaaag tccgtcaaac aaggccgaga ttatctcacc gtagagctga
     1441 caggcggcaa aaaacacaga ctcaaaggat ttttatatca tgacaatttc agcagagaac
     1501 ttttatcaga gcatatcaaa gcagaacgag agcgtagaaa ctctccaagt gaacttgcag
     1561 cagctatcaa tcaaactgga aagcttcgtg aagaatatcg cacaaagcga gaaacctatc
     1621 acagaaggca ccattctttt acaccaagta cagcaaacga caatagccct agaatcgcat
     1681 ctacgctcga ttttgatagc gataaagaat ctatcacccc aagcaataca aacgctcaca
     1741 acgagctata cggagcaaat acaagtatta caagcacagc tatgcaatat cgatatatcg
     1801 agcatcgaaa ccgtcacaac caacatcaat tcgttttcaa catcaatcga ggcgttacag
     1861 gagcacatca acagccaagt caaaagcctc aagatcgatc aaaagagctt acagaacagt
     1921 atccaagccc aagtccgaca agcaatagca aaccaaagcg aacaaatcca gagcgacatc
     1981 cagaccttaa tcacggaaaa attgagtact cagagagcaa cctacctcct atacggaatt
     2041 atagtttttg gagtgctgat tcttttaatg agtttatatc tcagttatca gaccagttca
     2101 aatctaaaaa cgatcaaagc acaacgcgaa gtgattcaac agaactcaca aataatttca
     2161 aatcaaaaca gcaacatgaa ttaattgcgg aagaaaacaa tgcagaccga aatcgacctt
     2221 tttttgaaag agcaaaacga cttattgatt caacaaaaca acttgttagc agagcaaaac
     2281 aacgtattga atcaacaagt gagttcatta aaaagcatct tgagcaaata cagcgaagtc
     2341 gagcagagct tacaaaacaa aatcagcaac ttgaataccg agagagaaac ccagcaggcc
     2401 ttgatcctgg ctatgaaatc agaactttca aaactcgaac tgggcagctt ttcggagcaa
     2461 ttacagcaag catttcaaaa caatgtgctg atgcaattac agacgccatt aacgagagca
     2521 ttaaatcaac tggatttaaa cagcgttgtc agcgatttag tgaagaccga attgaaaccc
     2581 attcttttga atatcaacac aaagcagacc gatctcaaga acgcactctt gaaaatctcg
     2641 caagtgaagg tacccgacaa cttttacaaa gatctaaaga tgcagagcaa gcaaatcgac
     2701 atgctcgtgg agacagtgga aatattagtc aaattaatca acaacttcgg ggactagaaa
     2761 aaacagtagc caaaattcga cttaagccaa agccaacgac agacttttat ctcaatttat
     2821 tgcgcaatga caattattac cccgactatg ttctttatca caaagaacta tgtgatgaac
     2881 agcaacaagc cttcaataac caggacacat tactattaat tgattacaca aagaaaaagg
     2941 cagaaaacct agaaacctac atgagccgag caagtcgaaa cttgaataaa tctgactatg
     3001 aaaaaattga acagatcatc aaaaatgatc aacgcatgct gaaatatctg caatgcgaga
     3061 agattttaga acggcaagat acccgttttg agcagcacag agctgcatac caaagctgtt
     3121 taaagcgctt tgagaatatc aaacttgata ttgagcaaat taaaaactcg tcctctcaac
     3181 ataatgtttc tcagaataca tatgaacctg aatacaaaac aaagccaaaa aatgacttag
     3241 attttaattt ttagttaatc taaacaatga tttaccgagc gagtgtctac gagcgactga
     3301 gcgatataaa aatttcgccc tgctctttct ctgattacaa gcttccttgt gctgctaaag
     3361 gagattaacc gagcaaagcg acgactctat atttgagtct agatcgagcg tagcgagtaa
     3421 aaaagcttta tgagcgaagc gaattccgag ttgcttttga tcttgctttt gacttttttt
     3481 aaatacacgt aaacataaac caaaaaattg ccccgtcgaa tcgagcgaaa gcgagattca
     3541 atagagtttg agcgtagcga aaaccaaggg caattttttc tatgtagtgt ttttaattat
     3601 ttttaatgtt tttaatgttt ttaagtattc cataagcctt gattctagag acttctagat
     3661 cagatataaa taggtttata gacctatatg aataggttta tagacctata tgaataggtt
     3721 tgtagaccta tatgaatagg tttatagatc atatgaatag gtttatagat tttctgatac
     3781 tttgcaatta tgtataccaa ttatatgagt taatcctcct gtatctcctg tatctcctgt
     3841 atctcctgta tctcctgtat ctcctgtatc tcctgtatct cctgtattta ctgtatttac
     3901 tgtatttact gtatttactg tatttactgt atttactgta tttactgtat ctcctgtatc
     3961 tcctgtatct cctgtatctc ctgtatttac tgtatttact gtatttactg tatttactgt
     4021 atttactgta tttactgtat ttactgtatt tactgtatga ataggtttat agaccccaat
     4081 aaataatttt aaatcaataa tatagattgc ttttaaccaa agtaattgtg tgtgttgaat
     4141 agactattta aatagtttat tctatgagct agttttaaat taattattag aaatgaaaaa
     4201 agaaatcgta aaagataata ctcttgtcca agctagctac acattagaca tagtcgaaca
     4261 gcgcctcatt cttttagcaa tcgctgaagc aagagaaaca ggacacggga tcacagaaaa
     4321 tagtttgtta gcgattcacg ctagtagcta tatcaatact tttaacgtag aaaagcatac
     4381 tgcatataca gtgctgagag atgcgtctaa aagcttattt aaacgttatg ttacgtatca
     4441 tgatatcaat cctaaaacag gaaaaaaccg aagctttcat tgccgctggg tggataaaat
     4501 tggatatgaa aaaaaatcag gaatcattta tttacgtttc actcaagaaa ttgtcccatt
     4561 aattacacga cttgaagaga atttcaccaa atatgattta cagcaagtat caaaactaac
     4621 tagcgcttat gctattcgac tttacgaatt actcattcaa tggcgatcca ctggtaaaac
     4681 ccctgttttt aatttaagta tttttcgcca gcagcttggt gttgaagaac atcaatataa
     4741 aactatgagt aactttaaga cctatgtttt agagtttgca ttagaacaag taaacaaatc
     4801 tacggacatc tcagctaaat atcaacagca taaaaatggt cgctctattt cgggtttttc
     4861 attcagtttt aagcataaaa aagtaactaa tcctgatagt gaaaataaga gagatccaaa
     4921 tacattagat gctttctcaa aaatgacaga tgctcaacgt cacttatttt ctaataaact
     4981 ttcgaaatta cctgaaatga gtaaatactc tcagggaacc gaaagctatc agcagtttgc
     5041 catacgcatt gctgaaatgc ttcaagatcc tactagattt gaagaattac atccttatct
     5101 tcaaaaagtt gggtttaaag ctgcctaaat tatagacttg atcatttgtc tattagacag
     5161 tctaatagac atcaactttt gacaagatta ggcatagttt tacaccatga aaagtctttc
     5221 agttcttgaa ttatctaagc tttacagcat aaatagacaa acaatttaca accaaataaa
     5281 taaaggaatt ttaagtaaaa attctgataa taagattgat ttagctgaag ctattcgagt
     5341 atttggtgaa cctattagaa aacaggatgt aaaagaacct gtaaaagtgg acagtccaaa
     5401 ttcggcagaa gttttactgc ttaaacaaca aatagacatg ctaaaaaatc agcttgagga
     5461 tgccaaagat cgggaatctt tctatcaaaa ccaaattgaa acgatgcaac gcttattaga
     5521 agccccaaaa cctaatatga ctacgtttac cgatcaagaa cccgtgcaag caagcatggt
     5581 caaaacagat cccgaaccgg acacagcaga agctaaacat gacggattga ctactccaga
     5641 gaagactgaa aataagcgga ttccagtacc ggagcacatt gaacaggaac cggaaaaaag
     5701 aggcttttgg agccgttttt ttagacccta tgattaacca cgattgttta actgatatga
     5761 acaaagttgt gaaacattga tcaaataata ggcgtggaac gtgaaaaatc ataaaaaaag
     5821 cccaccagag ggtatgtggg ctaaaatcat gtaactacag cattgatttc tatagataat
     5881 tataacgcat ttcgtataag gtgtattatg ttaattttag aaattctaaa aatatcttgt
     5941 aaaaattatc ttagtaataa tactggtttt gtagaacttg ataacaattt ggtcgtcgta
     6001 ctaccaataa aaaactggcg tatctttgaa tgaccatatg caccaatcac aatcatatca
     6061 ataacgtttt tttcttgata atccaatata gatttctcaa catcaccttc aagcacacta
     6121 tcaatgacat caaaaccatt atttgcaagt agctcttttg cccaagtgac ttgtgatgtc
     6181 atatcactat ttggcttacc aacatatacc acatgagctg tcagggtttt taataaaggg
     6241 ctatttgcaa ttttgtctac acaaatacgt gcagtaggac ttccatcaaa agcaattaaa
     6301 aatgactgag gtggactaaa cggtgaggat gtgattaaca cgggtttatg aagagcacga
     6361 acaacatttt caatatgggt tccaacttta ctggaattgt gctcggtttc agttccatgc
     6421 ttacccataa caagaactcg aatatcctct tccattccca tgattgtttc taaaagactg
     6481 ccatggcgtt gaaagctttg agcatcaata gagaacttct ccaatagata gttttttgct
     6541 tcccgaagaa gaatttggcc atgctctagc tcaattttcc ccttacgttc ttccagttca
     6601 accaactcgt ttagcaaggt ttctcgactt cctagaccaa ttgcaccact taaatcttgt
     6661 ggtgctttcg cttttggctt atcaatgaca tgcaataact gaagaggtga attcaattta
     6721 tcgctaaacc aagctgcata atcacaaacg gtatttgttg ctaatgatcc atcaatacat
     6781 gcaataattt ttgacataat tcatccctct tagtggctat ttaagcgatc taaagcatct
     6841 ggcttatcat ggataccaaa cttatcgact aacgttttac tggcttcatt cattccaatc
     6901 agttcgactt ctgttccttc acgtctaaac ttaatgacca ctttatctac ggcagcgacc
     6961 gcagacacat cccaaaagtg agcctgacta acatcaatca caacttttga aagtgcttct
     7021 ttgaaatcaa atgcagacgt aaatttatct gccgagctga agaacacttg acccgtcacc
     7081 gtataagtac gggtactttc aggttcatta aattcggact gaattgccat gtagcgttca
     7141 attttgttag caaaaaataa tgcagaaaga agaacaccga ctaatacgcc ataagccagg
     7201 ttatgtgtgg caacaaccac aaccactgtc gctatcatta cgatattgct agagaaggga
     7261 tattggcgaa tattgcgcag tgaatcccag ttaaatgttc cgattgaaac catgatcatt
     7321 acggcaacca gtgcagccat tggaatgact ttgagccagt cactgataaa caccaccata
     7381 attaacagga agacccccgc actaaaggta gataaacggg tacgaccacc agatttcaca
     7441 ttaataattg attgaccgat catggcacag cctgccattc cgcctaagaa gcctgaagcg
     7501 acattggcaa cgccttgtcc tttacactca cggtttttat cactggtggt atctgttaaa
     7561 tcgtcgacaa tcgttgctgt catcatagat tcaagcaaac cgacggctgc taatcccagt
     7621 gaataaggca aaataatagc taaagtttca agatttaaag gaatatcagg aagcaagaaa
     7681 attggaagag tatctggtaa ttgccccata tcacctacgg ttcttacatc taaaccaatg
     7741 aacaaggcaa agagcgtgag gagtacaata cagatgaggg gagagggtaa aagcttgcct
     7801 attactggga tataggggaa tagataaata atggctaaac caccaattgt catcgcatag
     7861 acatgccatg tcacattggt gagttcaggc aattgggcca taaaaattag aatagccaag
     7921 gcattcacaa agccaatcac aaccgacttt gacacaaagc gcatcaatgc acctaatttt
     7981 agatagcctg ctaggatttg aataaatcct gtcaaaattg tggctgctaa taagtattgc
     8041 agaccatgct ctttaaccag tgtgaccatt aataaagcca tagcaccagt tgcagcagaa
     8101 atcatccctg gacgaccgcc tacgaaagaa atcaccacgg caatacagaa tgaagcatat
     8161 aaccctacct tagggtcgac acctgcaata attgaaaagg caattgcttc aggaattaga
     8221 gccaaagcaa caacaatacc cgcaagaaca tccccgcgaa tattggagaa ccattgttct
     8281 cgaatagtgg ataacatgaa aatagcctat ttttttaaat tttaggaaag gctagtgatg
     8341 tgatatatga cacacaagct actacaccac atcactagac agtgatgagt tatttactgt
     8401 tttaagagat aaataaactt atggtggcgt aagtgtcata ttaagaaaga tatctatttt
     8461 ggagggtttc gggggcaatg atagcagtta ggcccttttc tatccaccct aaccgcagta
     8521 ctgaagtaaa agtaagaatg atcatagata attttagaat tgatttatgt ccatttaaca
     8581 taatggcgct tatacgaaac gcaaaataaa tagtgttata tttcaaaaag ttatgctata
     8641 agaaatcggg gacgcaaacc cgaaaagccg acttggaaac aagtcggctt ttttatgagc
     8701 ggttttgata gttcttgcca ataattttgt ttaaaaagta atcaattttg agatttttca
     8761 aattttatca atgcctcaat gtccaaaatt atccacattt tctgtggata aaatttaggc
     8821 tattttgtac gctttggctc acatgaaact cggccctttg cggctgtcca ggataggtga
     8881 attttcatat gattaattgg tcaggaacag gatgttcctt agaattataa agaatgagca
     8941 ccaggatggg gccagatacg acaagcacta agacagcaca gacctaaagt tccaataaaa
     9001 aaccctggca ggatgccggg gttttttatt atctaaactt tttaaattag ggttggatgg
     9061 gcatccagaa tgcgaattag agtcgctgca ggaccagtcg gatagcgacg gccttgttcc
     9121 cagttttgta gggttctcgc gctgatatgc aaacgtgcag caaattctgc ctgggttaga
     9181 gctatatgac ctagcagtca atgactatga acaacgaaag aaaaaagcca cagagaaaga
     9241 gagctttata gaaaatattg aagtgaaata attgctattt agttattggg taggatattt
     9301 caacttagga aaaaataaga aaaggcagct attgctagct gccttttgaa ttcattgcgt
     9361 caaataaccc actaaatctg ctaaaaaggg gacctagtag ttagcgttat gtgcgcctac
     9421 aacgtaatat tagctattat taactcaata aacaatagtt ttttgtaaca ggctaatttt
     9481 taatgcaaat ttcggacagt gcagcatttt ttaattctat atctcattca agaattaatt
     9541 cttataaaaa ctatttcggt tctcatctat cttcagaaga actaattgct tgttatcaat
     9601 ggaatgaaac agtatcagtt acttttttca aagtaattaa tctcattgaa attattctaa
     9661 gaaatagatt ccacttaacc ttaagtaatc acttttatag cattcccaaa gaacaagtta
     9721 ttgcaaaacg tgtacctgca agatcatctt tcagatatca aaatgttcca acgcttggga
     9781 ctgctgattc atgtaattgg tatgaaacta gattacttga aaaaaaaagc ttacaaaaag
     9841 tattagataa aactcatcat aaatcaggaa gtcctctcag agttccaata tttcctgatg
     9901 atttagttgc aggcttaact ctcggttttt ggtgttcatt gattcctaag aacttatcta
     9961 tgccatggga tcagcaatta aataatattt ttccaaacca tcgtttaacc aaatcattta
    10021 tagccaattc tcgaagttct ccaacaactg cttctaatcc ttggcttaat gttgacaatg
    10081 tacaacaagt atctaatcga ctcgaactct taaatgaatt caggaataga attgcacatc
    10141 atgaacctct atggaaatta cctgatcttt atgatgagat ccctacttct acagagaaaa
    10201 aagggactgt tatcgctaca aaaacaacta ataaaattga gacttttcaa agattaaatc
    10261 tacttcacca tagattcatt gaacttttaa aatggattga tatcaatttg gctcaagact
    10321 accaatcatc taaaaattac tattacctaa aatggctaat ctccaataat ggctttaata
    10381 gttatatcaa cagatatcag aactttgatc actgtttaca ttccagtata ttgaaacgta
    10441 atttaaattc tttactaaag cagcaagagt ctttcctgat atacagaaat catggaaata
    10501 tatatgctct acttcctatc acctagtttc tacttagaat acatgtcatt acctttaaga
    10561 tataatttgt cattatttca aattggttga tgacatggat catcaaattt ttcagaaagc
    10621 tatgcaatct ggtggaagac ttactgttaa aagcgcattg aatcctattt tatggctatg
    10681 cggtatcatt agcatcccaa ctattgctgc caacgttttt tacaccacct tgccaatatg
    10741 gatatctatc ataggttgtg cacctatttt aatggcaatg tttggtttta tggtgctctt
    10801 atttattgat cgcgataaat tgcaatccga agattatcaa attaaaaaaa tttcgttgga
    10861 aatgagtgag caaaagggcg acagtaatcc ccgcttagta ggtccctctg aaatacttac
    10921 tactaatcca gaacttccaa ccaccctaga agttctagaa catagtgatg aaaaatcttc
    10981 taaggtagcg aaatgaccaa atcatattta ttagtcttta gtgataaaac aggtactaaa
    11041 gatgaagtca aactagccct gaataaaatg actaaagtaa aaacttggcg tactgatatg
    11101 cctaattgct tttatgtaat atctgataat tctgcccaag aattgtatga agaatttaca
    11161 tccattcgtg gcactagtgg tcgattttta tttgcagaga ctgcttctaa tcgacaaggt
    11221 ctattaccat ctgatacttg gtatttattg aaaaataaaa agttgaaatc taaagaaacc
    11281 taagatttaa aaaaagtctg ccatacgcag acttttttta tttaacataa aatctcatct
    11341 acgaaatgca gcagcacgcg ctaggatcca attaaaactt taattgggtt cacatcaaat
    11401 actctattga tcacgataag ctcgaacttt ctcaaaaatt ggataaaagt ccagaccatc
    11461 atccttcata tctgaaatta cgatcgcaat cactgcaatg gtagtgggca ctttaccgtt
    11521 ctttttatag ttggtaattg aattcgcatt catatctaaa agcgctgcaa attctttaat
    11581 agacaggcca gacttaccta taagtctttg aaattcttcg taaggcatag tttaaaaagc
    11641 agaattgttc tgatgggaaa gtatagcaca gatcatttat ttacattcaa acacataaat
    11701 aatgtgttaa tcacaatatt tatgtgttta actattctta tcaagcactt ttactctttt
    11761 gataggactt gtttttatga accatttcgt acagcccaag cttcttattt cttctcaagc
    11821 aaaaaagatt ggattggcat taattaacga tcttgaactt tgtataacag cacgattggc
    11881 tttttatctg gatgtaaatc cttcctatga cggccatgag gcaacattca tttggatcga
    11941 atatttttta caagatcatc ctcaagtcga attttctgaa attaaagaag catttttaaa
    12001 acttattcct gaatgctgct aaacgatgat aatgaaatct gtattttatg agtcaaatac
    12061 agatttcaaa tctctctttt tacaatcaat acgattaatt tttaaccaaa tatagcatcc
    12121 atttttagaa caaaaataat agttttatcc ccccaatttt cccaaaaatt cacgccataa
    12181 ttaaaattta ccccattttt cgcttcaaaa agactttatt ttttcaatgc tgatataacg
    12241 agttcagacc ctagcacgtg acccaagcaa gcctacctgt attacatttt tttcaaaaat
    12301 tacatacagg gacttgttag gggtttcacc cctaagaccc cgaaaagcaa gagcaaaagc
    12361 acgttgagaa tctgacatgt tacgcacaaa aacaaagtca tttcgtttga ctgaattgca
    12421 actaaagcaa ctcgaaatcc atcttaaaaa agaaaactca gagttttcag attttcttca
    12481 ttcattaatt caacgtgaga tcatgcaaga cactgtcacc gttcaacaga tccaagacga
    12541 aataaaaccc cttagaacaa aagtaaaaac agtcgttgaa gtcgtcaaac gctaccaaaa
    12601 aactgaccct gatttattgc tcgagcttgc caaaatcggc aacaacatta atcagattgc
    12661 acgtgcatta aacatcatca aaaacgcaaa tccgcaggaa cagcgcaaac tcgatatttt
    12721 aaaaattttt ttagttttaa aagccatcca aactgaacta gaacaagttt tcccagctct
    12781 gccaaaaatc agccgacaaa gcccggaaag acttaaaaaa cagctgtctt cacttcaact
    12841 tgcggagcaa gacgaaagtg catattaaat tcctcgagca tggcaaaggc agtccagcaa
    12901 aagcatctgc atacctttta gatcaatatg accacaacgg tgatttgcgc gcaggggtgc
    12961 aagttctaaa aggggatgca accacgttta acgccatttg tgactccagt ccgcatcttt
    13021 ggaagtacac cagtggcgtc atcgcttggt caaaagaaga cgacccaacg gacgagcaaa
    13081 ttaaagaagt ccttaacgac tttgaacaac atgctttcgc aggattggaa caatcgcagt
    13141 atcacctttt tgcggtgcta catacagatc aagacggctc caagcacatc cacgttttaa
    13201 cccctcgact cgatatcgag agcggaaaat cactgaatat agccccgcca ggacatgaga
    13261 aacattttga tgcgctcaga gatcacttta acttaaaaaa caattggtca cgtccggatg
    13321 atttgctttt
//
