LOCUS       NZ_CP010354            11643 bp    DNA     circular CON 25-DEC-2023
DEFINITION  Acinetobacter johnsonii XBB1 plasmid pXBB1-3, complete sequence.
ACCESSION   NZ_CP010354
VERSION     NZ_CP010354.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03268929
            Assembly: GCF_001484935.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter johnsonii XBB1
  ORGANISM  Acinetobacter johnsonii XBB1
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 11643)
  AUTHORS   Zong,Z.
  TITLE     The complete genome of Acinetobacter johnsonnii strain XBB1 carrying
            blaNDM-1 and blaOXA-58 from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11643)
  AUTHORS   Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2014) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.6; Sspace v. 2.0
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina; 454
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/25/2023 04:54:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,995
            CDSs (total)                      :: 3,887
            Genes (coding)                    :: 3,684
            CDSs (with protein)               :: 3,684
            Genes (RNA)                       :: 108
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 203
            CDSs (without protein)            :: 203
            Pseudo Genes (ambiguous residues) :: 0 of 203
            Pseudo Genes (frameshifted)       :: 103 of 203
            Pseudo Genes (incomplete)         :: 102 of 203
            Pseudo Genes (internal stop)      :: 46 of 203
            Pseudo Genes (multiple problems)  :: 41 of 203
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP010354.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11643
                     /organism="Acinetobacter johnsonii XBB1"
                     /mol_type="genomic DNA"
                     /strain="XBB1"
                     /db_xref="taxon:1242245"
                     /plasmid="pXBB1-3"
     gene            complement(460..2136)
                     /locus_tag="EHS42_RS19370"
                     /old_locus_tag="RZ95_19220"
                     /old_locus_tag="RZ95_RS19170"
     CDS             complement(460..2136)
                     /locus_tag="EHS42_RS19370"
                     /old_locus_tag="RZ95_19220"
                     /old_locus_tag="RZ95_RS19170"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="WP_058952630.1"
                     /translation="MSTWLLGNTTVRSPFRLIDGLKVFASTNGDIRGTKEKELVFCKAL
                     VDGGIISSNFEAKDTSGFSDTTYSVGRKWRSALEKLGFIEQFNQIYILTENGRNLLNSQ
                     TLQSDQECYLRSLILYSCEAQNSDDSIGFFSPLMLTLHIMKELENRTGTSKISFQEMAA
                     VIQLTFSYSDINQCVDEILTIRSNRQASSSKKKFDRELYQSKSAKAEIQAPSIKDYADT
                     NLRYLKSTGLFTASGKGICFIDDKKIVIDKLIEMYGTFDISQSDLKIQKGAPLPTDHKE
                     TNILLAAQLEETLNRNRILFEKNSSILQAPIGEIKNYRYHLEELLFENNEKKFAENQKN
                     EWDEILAYMDVLISPRPMSIEIGDKEISIPSGEKPAYFEWVLWRAFLALNHLIIEPQQC
                     RRFKVDQDFKPIHHAPGGGADVIFEYENFKILGEVTLTSNSRQEAAEGEPVRRHIAVET
                     VNTPDKDVYGLFLALTIDTNTAETFRHGAWYHQEELMDVKILPLTLGSFKKYLESLREK
                     NQLEAGIFDLKKMMDESLKLREALTAPQWKNEITNKFTRAI"
                     /name="AlwI family type II restriction endonuclease"
     gene            complement(2185..4104)
                     /locus_tag="EHS42_RS19375"
                     /old_locus_tag="RZ95_19225"
                     /old_locus_tag="RZ95_RS19175"
     CDS             complement(2185..4104)
                     /locus_tag="EHS42_RS19375"
                     /old_locus_tag="RZ95_19225"
                     /old_locus_tag="RZ95_RS19175"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039635.1"
                     /GO_function="GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity [Evidence
                     IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="WP_058952631.1"
                     /translation="MNKPLLVNLSDAVPQKIKCKPILKWAGGKTQMLDSILPRFPNYYG
                     KYIEPFFGGGAVFFALQPENAVIADSNPELINLYRTVADDVDAVIAELKKFKNTEEMFY
                     EVRSQDRETLSSVEAAARTIYLNKTCFNGLYRVNKKGHFNVPFGKYKNPKILDEDNLRV
                     ASILLSKAKIVCSDYLDVLENFACPNDLIFLDPPYVPISEYADFKRYTKEQFYLEDHEN
                     LAKAYKALSDKGCHVFLTNSNHSIVENLYHGFKYEVIQTKRHISCDSKTRKGEDVIISN
                     TQKNEKFSLNLEKISTLPEQIKLYPSTRYMGSKQKLLPYILGIVEQFNSPILVDLFSGS
                     GIVSYLFKTLGRQVITNDYMNMSHTFTKAMVENNHVILSDEKARSLLIEKYPIDDFVQK
                     KFKDLYFSDDENLLIDIIRSNIPKLDNEYEQAIARSALIRACMKKRPRGIFTYTGHRYD
                     DGRKDLVLTLEEQFLNAVEAINQSIFDNHQSNISYRKNALDVELPDNCLIYIDPPYYST
                     CSDNEYVRRYHFVEGLSLDWKEIEIQEHTKTKKFKSYPTPFSSRKDVYVAFDTLFEKYK
                     ENILIISYSSNSLPNMDEMLVMLRKYKENVDVIPIDYKYSFGNQATKVGDNRNTVQEYL
                     FVAY"
                     /name="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
     gene            complement(4097..4348)
                     /locus_tag="EHS42_RS19380"
                     /old_locus_tag="RZ95_19230"
                     /old_locus_tag="RZ95_RS19180"
     CDS             complement(4097..4348)
                     /locus_tag="EHS42_RS19380"
                     /old_locus_tag="RZ95_19230"
                     /old_locus_tag="RZ95_RS19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146947.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_058952632.1"
                     /translation="MPYEEFQRLMGKAGLSIKEFATLLDMNPNSITNYKKIGKVPTHIA
                     VLVYLLSSMKDEGVDFYPIFEKVKEYNCGTNEGKIVSE"
                     /name="helix-turn-helix transcriptional regulator"
     gene            4498..4743
                     /locus_tag="EHS42_RS19385"
                     /old_locus_tag="RZ95_19235"
                     /old_locus_tag="RZ95_RS19185"
     CDS             4498..4743
                     /locus_tag="EHS42_RS19385"
                     /old_locus_tag="RZ95_19235"
                     /old_locus_tag="RZ95_RS19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004282220.1"
                     /translation="MYSYPDSNTEKKIALMIINDFFIQKAHELWIFLQLDQCFNDYEAT
                     LIWTRRYLEKHPECEYSDIQKAFVSCFPEHFFSFDC"
                     /name="hypothetical protein"
     gene            complement(5057..5302)
                     /locus_tag="EHS42_RS19390"
                     /old_locus_tag="RZ95_19240"
                     /old_locus_tag="RZ95_RS19190"
     CDS             complement(5057..5302)
                     /locus_tag="EHS42_RS19390"
                     /old_locus_tag="RZ95_19240"
                     /old_locus_tag="RZ95_RS19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582672.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_058952633.1"
                     /translation="MPYEEFYRLIGKAGLSIKEFAELLGMKPNSITNYNKVGFVPSHLA
                     VIVSLISTMKDEGFDFYPILEKVQAYEKARIESQSK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            5457..5699
                     /locus_tag="EHS42_RS19395"
                     /old_locus_tag="RZ95_19245"
                     /old_locus_tag="RZ95_RS19195"
     CDS             5457..5699
                     /locus_tag="EHS42_RS19395"
                     /old_locus_tag="RZ95_19245"
                     /old_locus_tag="RZ95_RS19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058952634.1"
                     /translation="MNYFTQPQLFISAKAKKLGLQLIQELYIQRSARLAFYLDVNPSFD
                     GHEAVLFWTEHYLVEYPDATFSDIQQKFIQSVPES"
                     /name="hypothetical protein"
     gene            complement(5756..6133)
                     /locus_tag="EHS42_RS19400"
                     /old_locus_tag="RZ95_19250"
                     /old_locus_tag="RZ95_RS19200"
     CDS             complement(5756..6133)
                     /locus_tag="EHS42_RS19400"
                     /old_locus_tag="RZ95_19250"
                     /old_locus_tag="RZ95_RS19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_081316870.1"
                     /translation="MSDIQEKKDAVLKKLTKAQQKKLSLQKQIDYLKKEQEQIQEQAKQ
                     KQNELKLKQQKLLKEAKAQERKDDTRRKILLGAWVLNKLKNDESFKHQLADFEQFLANE
                     SKTEENRLKDKNLFNGLLWDD"
                     /name="hypothetical protein"
     gene            6392..8050
                     /gene="mobQ"
                     /locus_tag="EHS42_RS19405"
                     /old_locus_tag="RZ95_19255"
                     /old_locus_tag="RZ95_RS19205"
     CDS             6392..8050
                     /gene="mobQ"
                     /locus_tag="EHS42_RS19405"
                     /old_locus_tag="RZ95_19255"
                     /old_locus_tag="RZ95_RS19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_058952635.1"
                     /translation="MAIFHLNESNISRSDGRSAVACAAYRACEKLLDHTFGKTQDYTRK
                     KGLEYKSIYAPEHTNEKLLDRQTLWNEVEKKEFNANGSMKANARLAKEYTCALPHELTH
                     KERIKIVDDFCRDFVKKHNVIVDACIHAPHDHDDETDNKNYHVHIMFTTRLVNEKGELG
                     KKQRTFNDDGPKILKDSRATFAHVVNTALENAGLEERIDHRSYKDQGLDFLEPTAHEGH
                     EVTALRRQGIDTEISLKNDVIKAKNLETAREYQQIIKGLDQEIIVPNKLEDQIAELEKE
                     LRLTAAEEQKLLAELANLDREEELLQEKLAQQIDNAYDNFIEQQSQYIEFAKRFYAKGD
                     HTNKQLELIDKQLEKSQKWLSKQNGFYYSNGLFYDNYHHQRIDVSTPYYFTTHHKTETK
                     KEEVMKQYRKDVAKLSDDFDIENTVSALKQNAKKLTDNAIELPVPAPTFMQKLRREYVH
                     SFDTLDDFKTDMKPVFDEYRAETLRAEKEKQKKIEEEENLEAFRQRTAEQQRKRDAERE
                     MKLRREERQQLRQSWANEPEPEKKPKKDNGFDLSM"
                     /name="mobQ"
     gene            8683..9582
                     /gene="repM"
                     /locus_tag="EHS42_RS19415"
                     /old_locus_tag="RZ95_19265"
                     /old_locus_tag="RZ95_RS19215"
     CDS             8683..9582
                     /gene="repM"
                     /locus_tag="EHS42_RS19415"
                     /old_locus_tag="RZ95_19265"
                     /old_locus_tag="RZ95_RS19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650500.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_171253726.1"
                     /translation="MMSTSKKELVVKSNQVIEASYQLSSTEQRIVLAAISKISRAEDIT
                     DDEIYRVTIDDLKKLGVHEKTAYRDLKDGVNRLYDRSINLAIDDESIKMRWIQSIRFLE
                     SKSVVGIRFSKEILPFISNLSREFTKYSLSDIAGMSSAYAIRIYELLSQYRSIGKREIS
                     IESLRGMLELGKRYPLSADLKRWVIDTAVDQINEHSPLNVSYEQIKTGRKVTHIHFKFK
                     EKLKSIEHKSEQNNFYKLTDAQINMFGNQLSRLHEVSHLAQQGESYDDLAIKIKDMLRD
                     PIQQKQLIPHLKNLGFKA"
                     /name="repM"
     gene            9659..10276
                     /locus_tag="EHS42_RS19420"
                     /old_locus_tag="RZ95_19270"
                     /old_locus_tag="RZ95_RS19220"
     CDS             9659..10276
                     /locus_tag="EHS42_RS19420"
                     /old_locus_tag="RZ95_19270"
                     /old_locus_tag="RZ95_RS19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_058952637.1"
                     /translation="MKSLSVIELSNLYGMTRQAIYKHINKGNLSKNSDGKIDLSEAIRV
                     FGEPSRNVNGSQTTETLKLSEVHLLEQQVYMLQKQLEQAHEREQFQREELKAKNDQLHI
                     KDEQIEAIQRLLEAPKTYTSTYIDPKSDTATDTRSKSDSNYDGLTTQQKEAPIQAEIQT
                     QPKDTGLTNPVETENKRIPVPEHIEQEPEKRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            10845..11564
                     /locus_tag="EHS42_RS19425"
                     /old_locus_tag="RZ95_19275"
                     /old_locus_tag="RZ95_RS19225"
     CDS             10845..11564
                     /locus_tag="EHS42_RS19425"
                     /old_locus_tag="RZ95_19275"
                     /old_locus_tag="RZ95_RS19225"
                     /inference="COORDINATES: protein motif:HMM:NF024895.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="WP_058952638.1"
                     /translation="MKRLLLVTAISFSSLTYADSGFYTSLKAGISDTKFEDYKLTLAQG
                     DESIIFKHKDADESIYPNISAAIGFDFSKVSKVNARAELEYTYKDKATFAPNISSGIFN
                     GNEEQAPPNIPSYVVNELRTQSLMLNGYYDFKNTTKFTPYLSAGLGVSRIENDISTNPE
                     IFNSSEHISTDTNNNFAWTAGAGVAYQVTKDVALDLAYRYVDAGEIETSLSFDGANFKN
                     TADLISHDYSLGIRYNF"
                     /name="outer membrane beta-barrel protein"
     Typing          8687..9582
                     /name="rep_cluster_1357"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.754"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggaggttata cgcaatacac ttgtatatta atttaaatat caattactta aaaatttcac
       61 cctgatcaca aaatgcacaa aaaatggctt ttgtaataag ttggtgagtc tttaagcgaa
      121 tctgtaacat tttgccaaga aatttgatta ataaatgatc aatttggcaa tcagtgccca
      181 tttcatgcgg attcaggttc taaaactatc cccaattgct ggggataaaa tttaggctat
      241 tttgtacact ttggctcaca tcaatctcgg ccctttgctg ctatccgcga tactggaatt
      301 ttcctatgat gaagtcatag agagcaggat gctccaaaca acaagaataa gaaagatcgc
      361 accaggaacg tgaggtaaga caggagtcat acttagcaac ccaatatgaa aaaccccggc
      421 aggatgccgg ggttttttat ttttaggaaa aataaccaat tagatagctc tagtaaactt
      481 attagtaatc tcatttttcc attgtggagc cgttaaagcc tctcgcaatt ttaaactttc
      541 atccatcatt ttcttcaagt caaaaatacc tgcttccaat tgatttttct ctcttagtga
      601 ttccaaatat tttttaaaac ttcctaaagt taatggcaaa attttcacat ccataagttc
      661 ttcttgatga taccaagcac catgcctgaa tgtttctgct gtattagtgt caatagtaag
      721 tgcaagaaac aaaccataaa catctttatc aggtgtattc actgtttcta ccgcaatatg
      781 tcgtcttaca ggttcacctt cagctgcttc ctgtctagag tttgacgtta atgtaacttc
      841 tccaagaatt ttaaaatttt catactcaaa gattacatca gcaccacccc ctggagcatg
      901 atgaataggt ttaaagtcct gatccacctt aaatctccga cattgttgcg gctcaatgat
      961 taaatgattt aaagctaaaa atgctctcca caagacccat tcaaaatatg ctggtttttc
     1021 tcctgacgga atagaaatct ctttatcacc aatctcaatt gacattggtc taggtgaaat
     1081 tagcacatcc atataagcaa gaatttcatc ccactcattc ttttgatttt cagcaaattt
     1141 tttctcatta ttttcaaaaa gtagctcttc taagtgatat ctataatttt tgatttcacc
     1201 tattggtgct tgtaatatag aactattttt ttcaaataaa attctatttc tatttaatgt
     1261 ctcttctaat tgcgcggcta ataatatgtt tgtttcttta tgatctgttg gtaaaggagc
     1321 acctttctga atttttaaat cagattgact tatatcaaag gttccataca tttctattag
     1381 cttatcaatg actatttttt tgtcatcaat gaaacaaatt cccttaccag aggcagtaaa
     1441 taaacctgta gattttaaat atcttaaatt agtatcagca tagtctttga tactaggagc
     1501 ttgaatttct gctttagcag acttcgattg atataattct ctatcaaatt tcttttttga
     1561 tgaagatgct tgcctattac ttctgatagt aagaatttca tcaacacatt gatttatatc
     1621 tgaataacta aatgttaatt ggataactgc tgccatttct tgaaaggaaa ttttactagt
     1681 tcctgttcta ttttctaatt ccttcatgat gtgaagcgtt aacatcaaag gagaaaaaaa
     1741 tcctattgaa tcatctgaat tttgagcttc acaagaatat agaattaatg accttaaata
     1801 gcactcctga tcagactgta aagtttgact attgagaaga ttcctaccat tttcagttaa
     1861 aatataaatt tgattaaatt gttctataaa ccccaatttt tctaacgcgg aacgccattt
     1921 tctaccaaca ctatatgttg tatcactaaa gccagaggta tcttttgctt caaaattaga
     1981 actaataatt ccaccatcaa ccaatgcttt gcagaatact aattctttct cctttgtccc
     2041 cctaatgtcc ccattagttg aagcaaaaac ttttagccca tcaatcagtc tgaaagggct
     2101 acgaactgtt gtatttccaa gaagccacgt gctcataatt ataaaaaaaa aggaataaaa
     2161 ataagatttt attccttttt ccctttagta agctacaaat aagtactctt gaactgtatt
     2221 acggttgtcc ccaaccttag ttgcttgatt accaaatgaa tacttataat ctattggtat
     2281 cacatctacg ttttccttat atttacgtaa cataaccaac atttcatcca tattcggaag
     2341 gctattagat gaatacgata taataagaat attttcctta tacttctcaa ataacgtatc
     2401 aaaagcaaca tagacatctt ttcgagatga aaaaggcgtt ggataagatt taaatttctt
     2461 tgtcttggta tgctcttgaa tttcaatttc tttccaatcc agagataacc cttcgacaaa
     2521 atggtacctt ctaacatatt cattatccga acatgtcgaa taataaggag gatcaatata
     2581 aatcaaacaa ttatcaggta gttcaacgtc taaagcattt tttcggtatg aaatatttga
     2641 ttgatgatta tcaaaaatag actgattaat tgcttctaca gcattcaaaa actgttcttc
     2701 taatgtcaga actaagtctt ttcttccatc atcataacga tgaccggtgt atgtaaaaat
     2761 tccacgaggt ctctttttca tacacgcgcg aatcaatgca cttctagcaa tcgcttgctc
     2821 atattcatta tcaagcttcg gaatgttaga tcgaataata tcaatcaaca aattctcatc
     2881 atcagaaaaa tatagatcct taaatttttt ctggacgaaa tcatctatcg gatacttttc
     2941 tattaacaat gatctggctt tttcatcact taaaatcaca tgattattct ctaccatcgc
     3001 ttttgtgaaa gtgtgtgaca tattcatata atcatttgtt attacttgcc tacctagcgt
     3061 tttaaacaaa tagctgacta tgcctgatcc agagaataaa tctactaata ttggtgaatt
     3121 aaattgttca acgataccta aaatataagg gagtaatttc tgcttagacc ccatatatct
     3181 tgttgaagga tataatttta tttgttcagg taatgttgaa attttttcca aattcaaact
     3241 aaatttctca tttttttgag tatttgagat aattacatct tcaccttttc gtgttttcga
     3301 atcacatgaa atatgtcttt ttgtctggat aacttcatac ttaaatccat gataaagatt
     3361 ttcaactata gaatgattag agttagtcaa aaaaacatga catcccttat cgctcaaagc
     3421 tttatatgct ttagctaagt tttcatgatc ttctaaataa aattgctctt tagtatatct
     3481 tttaaaatct gcatattctg aaataggtac ataaggagga tctaaaaaaa tcaaatcatt
     3541 tggacaggca aaattttcta aaacgtccaa ataatcagaa caaacgattt ttgccttgga
     3601 taataaaata gaagctactc ttaaattatc ctcatctaaa atcttaggat ttttgtattt
     3661 tccaaaaggc acattaaagt gtcccttttt attcactcta tacagaccat taaaacaggt
     3721 tttattcaag taaattgttc gagcagcagc ttctactgaa gataacgtct ccctatcttg
     3781 agaacgaact tcataaaaca tttcttcggt atttttaaat ttttttaact cagcaattac
     3841 agcatcaaca tcatctgcga ctgtacgata taagttaatc agttcaggat tactatcagc
     3901 aattacggca ttctcaggtt gtaatgcaaa aaagactgct cctcccccaa aaaaaggctc
     3961 aatatacttt ccataatagt ttggaaatct aggcaaaatt gagtctaaca tttgcgtttt
     4021 accaccagcc catttaagaa tcggcttaca tttaattttt tgtggtacag catcactcaa
     4081 gtttactaaa agaggtttat tcacttacta ttttcccctc attagtacca caattatatt
     4141 cttttacttt ttcaaaaata gggtaaaagt ctacaccctc atctttcatg ctagaaagta
     4201 agtaaaccag aacagcgata tgagtaggta cttttccgat ctttttatag ttagttatgg
     4261 agtttggatt catgtccaat aaggttgcaa attccttaat tgaaagacct gctttcccca
     4321 taagtctttg aaattcttcg tatggcatcg tgataaatac aatctgtttg aattagaagt
     4381 ataacacaga ctataaaact caaaaaaaca cataaaaaat gtgacaaaca cattttttat
     4441 gtgttataaa tagcttatca actaacctat ttatccttac tatcggagaa atgtaatatg
     4501 tactcatacc cggattcaaa cactgaaaag aaaattgctc tcatgatcat caatgacttt
     4561 ttcattcaaa aagcccatga actgtggatt tttttacaac tcgatcagtg ctttaatgat
     4621 tatgaagcca cactgatttg gactcgacgt tatttagaga agcatcctga gtgtgagtac
     4681 agcgatattc aaaaagcctt tgtttcttgt ttcccagaac acttcttcag ctttgactgt
     4741 taatttatga gaggcacggt aaaaaatcga aatgggtaag taaatcccaa ataacagcca
     4801 gttcatacaa ctggcttttt attgttacgt ataaatctaa gactttcttt gaacatacaa
     4861 aaattgatgt tccacggctt ctaagacatt gattttaggt tcaggattgg ggccgccatg
     4921 acgttacttt agggtgtact ctaaatttac ataacatctc atctacgaaa tcacaaaaat
     4981 gggggcttat cgctcccatt tcattgtagt actctttgat caacaacaac cccaaaaaat
     5041 ccagcgtaat ctttttctat ttagactgac tttctatacg ggctttctca tatgcctgta
     5101 ctttttctag gataggataa aaatcaaaac cctcatcctt catcgttgat atgagcgata
     5161 cgatcacagc caaatgagaa ggcacaaatc caactttatt ataattcgta atagaattag
     5221 gcttcattcc aagtaattca gcaaattctt taattgaaag acctgcttta ccgataagtc
     5281 tatagaattc ttcgtaaggc atgagattca aatttaacaa ataataaagt gaaagtataa
     5341 cataggctat ttctttacac agaatattaa aaaacacata aaaatatgtt tacaacacat
     5401 atttttatgt gtttaattat ccatatcaag cctatgcttc tgtatcggat ttttttatga
     5461 attacttcac tcaacctcaa ttatttattt cagcaaaagc aaaaaaatta ggattacagt
     5521 taatccaaga gctttatatt caacgtagtg cccgtttggc cttttattta gatgtgaatc
     5581 cctcttttga tgggcatgaa gctgttttat tttggactga acattattta gtagaatacc
     5641 cagatgcaac tttctcagat attcagcaaa aatttataca atctgttcca gaatcctaaa
     5701 ctttttagtg actgtgctta ccaggacagg tcttggtatt gaaaggtcat caaaattagt
     5761 catcccataa aagcccatta aatagatttt tatccttcaa tctattttct tctgtcttgc
     5821 tttcattagc taaaaactgc tcaaagtctg caagttgatg tttaaacgac tcatcatttt
     5881 tcagcttatt tagaacccat gcaccgagca aaattttacg tcgagtatca tctttacgtt
     5941 cttgtgcttt ggcttctttt aaaagttttt gttgtttcaa cttaagttca ttttgcttct
     6001 gtttagcctg ctcttgtatc tgttcttgtt ctttttttaa ataatcaatc tgtttttgaa
     6061 gagataattt tttctgctgt gcttttgtga gcttctttaa aacagcatct ttcttttctt
     6121 gaatatctga cataatatta aaccgtgatg tgcaataaac aaacaccatc ataaagtcaa
     6181 aaaataaaac cagcaatatc aaacgtagcg aagcggagtt ttcccgtaac gaagtgaagg
     6241 gcgcacttac ccacttttca tcttcgatag aaaaatggaa gtacgagagg ggacacccct
     6301 ctaccccaag ccctccccta aaggatgggc agcaactttt tcaaagttgc aaaaagcaaa
     6361 accacgatat tcaaaatgta ggttttttaa aatggcaatt tttcatttga atgaaagcaa
     6421 catttcaaga agtgacggac gttcagccgt tgcttgtgct gcttatcgtg catgcgaaaa
     6481 attattagac catacctttg gaaagaccca agattacacc cgaaaaaaag gacttgagta
     6541 caagtcgatt tatgcccctg aacatacgaa tgaaaaatta ttagatcgcc agacgttgtg
     6601 gaatgaagtc gaaaaaaaag agttcaatgc aaacggttcg atgaaagcaa atgctcgact
     6661 ggcaaaagaa tatacctgcg ctttaccgca tgagctaaca cacaaagaac ggattaaaat
     6721 cgttgatgac ttttgccgtg acttcgtgaa aaaacacaat gtcattgtcg atgcatgcat
     6781 ccatgcacca catgaccatg atgacgaaac agacaataaa aactatcacg tacatatcat
     6841 gttcactacc cgactcgtga acgaaaaagg tgaacttggc aagaagcaaa gaacgttcaa
     6901 tgatgacggc ccgaaaattt taaaagactc ccgagccact tttgcgcatg ttgtgaatac
     6961 ggcattagaa aatgcaggac ttgaagagcg aatagaccac cgcagctaca aggatcaggg
     7021 actcgatttt cttgaaccaa cagcgcacga agggcacgaa gttacagcac ttcgcaggca
     7081 gggcatagac actgaaatca gtctaaaaaa tgatgtgatc aaagcaaaaa atttagaaac
     7141 ggcaagagaa tatcaacaaa ttatcaaagg acttgatcaa gaaattattg tacccaacaa
     7201 gttagaagat caaattgcag agctggagaa agaactccgt ttaacagcag cagaagagca
     7261 aaagcttcta gcagaattag caaacttaga tcgagaagaa gaacttcttc aagaaaagct
     7321 agcacagcaa atcgacaatg cttacgacaa tttcatagag cagcaatcac aatatattga
     7381 gtttgctaaa agattttatg ccaaaggtga ccatacaaat aagcaacttg agctgattga
     7441 taagcaactc gaaaaatctc aaaaatggct ttcaaaacaa aatggttttt attattcgaa
     7501 tgggctgttt tatgacaact atcaccacca acgcatagac gttagtacac cgtattactt
     7561 cacaacgcac cataaaacag agactaaaaa agaagaggta atgaagcagt acagaaaaga
     7621 tgtagcaaag ctttcagatg attttgatat tgaaaacaca gtcagcgcat taaaacaaaa
     7681 tgcgaaaaaa ttgaccgaca atgcaattga gcttccagtc ccggcaccga cattcatgca
     7741 aaaattaaga cgtgagtatg ttcacagctt tgacacactc gatgacttta aaactgatat
     7801 gaaacctgtt ttcgatgaat acagagcaga gacattacgc gctgaaaaag aaaagcagaa
     7861 aaagattgag gaagaagaaa atttagaagc ttttagacaa cgcacggctg agcaacaacg
     7921 caagcgcgat gccgaacgtg aaatgaaact gcgtcgtgaa gagcgtcaac agttaagaca
     7981 gtcgtgggca aatgagccag aacccgagaa aaaacccaag aaggacaatg gtttcgatct
     8041 atccatgtaa aaacatgtcg tagaatcgca cagaattgcg aattttggat ttttttatga
     8101 aaaatgaccc taggtagcta aaaataatta acgatcttaa atcgaaaaat aatagcaatt
     8161 agcgagtgtc tacgagcgac atgacaaaaa tttgcttgcc ttttctctga ttacgatctt
     8221 cctatccata taacacggtc agaaaagagc atcccgaatg ggtgcgaacg aataatatga
     8281 aaattttgtt cgagcgaagc gagtaaagaa gctttatgag cgaagcgaat tccgagttgc
     8341 ttttgatctt gcttttgctt tttctcaaat tcacgaaaat atgaaccaaa aaattgcccc
     8401 gtcgaatcga gcgaaagcga gattcaatag agtttgagcg tagcgaaaac caagggcaat
     8461 ttttaaattt gctcttttta aatatatttt aaatctttta aatcttttag gtggcttgaa
     8521 agccatgtat atcaatgctt tcagctttta actatgaggg attgactact aactatgagg
     8581 gattgactac taactatgag ggattgacta ctaaactatg agggattgac tactaactat
     8641 gagagaatta tattactcat agttaggttt atagaaatag aattgatgag tacatctaaa
     8701 aaagaattag tagttaaatc aaatcaagta attgaggctt catatcaatt aagctctaca
     8761 gaacagcgta ttgtgttagc tgctatcagt aaaattagtc gagctgaaga tattacagat
     8821 gatgaaatct atcgcgtaac cattgatgat ttaaaaaaac ttggggttca tgaaaagacc
     8881 gcttatagag atctaaaaga tggtgtaaat cgtttatatg ataggtcaat taatttagct
     8941 attgatgatg aatcaattaa aatgagatgg attcaatcca tacgtttttt agagagtaaa
     9001 agtgttgtag gaattagatt ttctaaagaa attttgccat tcatttctaa tctaagccgt
     9061 gaattcacta aatattcgtt atctgatatt gctggtatga gtagtgcata tgctattcgt
     9121 atttacgaat tattaagcca ataccgttca ataggaaaac gagagatttc catcgagtct
     9181 ctaagaggaa tgttagagtt aggaaaaaga tatcctcttt ctgctgattt aaaaagatgg
     9241 gttattgata ctgctgtaga tcaaatcaat gaacatagcc cattaaatgt atcttatgag
     9301 caaataaaga caggtcgaaa agtcacacat attcacttca agtttaaaga aaaattaaaa
     9361 agtattgagc ataaatccga acaaaataat ttttataaat tgacagatgc acaaattaat
     9421 atgtttggta atcagctttc acgcttacat gaagtatctc acctagcaca acaaggcgaa
     9481 agttatgatg acttagctat taaaattaaa gatatgctgc gagatccaat acaacaaaaa
     9541 caacttattc ctcatcttaa aaatttaggt tttaaagcat aaagatagat tgtgtatgta
     9601 gcggttgact gtttgttaat gtatcttagt tcacataata gacaaccaaa attacaagat
     9661 gaaatcacta tctgttattg agctctcaaa cctctatgga atgactcgtc aagccattta
     9721 taagcatatt aataaaggga acttaagtaa gaatagtgac ggtaaaattg atctttccga
     9781 agctattcgt gtctttggtg aaccttctag aaatgtgaac ggttcacaga caactgaaac
     9841 actaaagttg tcagaagttc acctgctaga acagcaggtt tacatgcttc agaaacagct
     9901 tgaacaggcg catgagcgtg agcagtttca acgtgaggag ttgaaagcta agaatgatca
     9961 attacacatt aaagatgaac aaattgaagc gatacaacgc ctattagaag ctccaaaaac
    10021 ttatacatct acatatatcg atccaaaatc agatacagca acagatacta ggtcaaaaag
    10081 tgattcgaac tatgatggat tgactactca acaaaaagaa gctcctatac aagcagaaat
    10141 ccagactcaa cctaaagata ctggattgac taatccagtt gaaacagaaa ataagcggat
    10201 tccagtaccc gagcacattg aacaggaacc ggaaaaaaga ggcttttgga gccgtttttt
    10261 tagaccctat gattaaccac gattgtttaa ctgatatgaa caaagtcgtg aaacattgat
    10321 taaagaatag gcgtgaaacg tgaaaaatca taaaaaaagc ccgaacaggg atgttcgggc
    10381 tataaactag aaactacagc attgatttct aaggaaaatt ataacacgtt tcgtataagg
    10441 tgtattatgt taatttaggg aaacttaaca ttttgttctt atagatacta ctttaatgat
    10501 tatttcctaa aaggatcatt cctccattct ttatatagcg aatagactaa ccaaatagct
    10561 aatataagta aaaagagtat acatataaga tagaaatcca tgattaataa ctagtaaaaa
    10621 tataattatc aattacacat aaattaacag aaattagcaa ttattttacg aaaaaaaatt
    10681 ggttatatta ctggatttaa aagaaattgt ttaaaaagta gcaaaaagat aaaatttgat
    10741 aagcaaaatg aaacctatac tcttaaatat taatactaaa gaagtatcaa aaaataacaa
    10801 aaaagatgta caattctcat aataattaca gagaacaaac acttatgaag cgtcttctat
    10861 tagtaactgc catttcattt tcaagtttaa cttatgcaga tagcggtttt tatacatcat
    10921 taaaagctgg tattagcgat acaaagtttg aagactataa attgacactg gctcagggtg
    10981 atgagagcat catctttaaa cataaagatg cagatgaaag tatttaccca aatatctcag
    11041 ctgctatcgg ttttgatttt agtaaggtaa gtaaagtaaa tgctcgtgct gaactagaat
    11101 atacatataa agataaagct acattcgcgc ctaatatcag ctcaggtatt ttcaatggta
    11161 acgaagagca agcaccacca aacattccta gttatgtagt aaatgaactt agaactcagt
    11221 ctttaatgct aaatggctat tacgatttta aaaatacaac taaatttacg ccatatttaa
    11281 gtgctggctt aggtgtaagc cgtattgaaa atgacatttc aactaaccct gaaatattta
    11341 attcttctga gcatatctct acagatacaa ataataactt cgcttggaca gcaggtgcag
    11401 gtgtagctta tcaagtaact aaagatgtgg ctcttgattt agcttatcgt tatgtcgatg
    11461 ctggagaaat tgagactagc ctatctttcg atggtgcaaa ttttaaaaat acagcggact
    11521 taatatcaca tgactactct ttaggtattc gttacaactt ctaagatatt taagagaata
    11581 gggggatgaa gtatccccct attcttacaa tagataaatt ttttatcccc catttaacat
    11641 aat
//
