LOCUS       NZ_CP010245             7618 bp    DNA     circular CON 12-JUN-2024
DEFINITION  Escherichia coli strain S56 plasmid C, complete sequence.
ACCESSION   NZ_CP010245
VERSION     NZ_CP010245.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03252458
            Assembly: GCF_001901445.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Jiang,J., Xu,Z., Zhou,Y., Zhao,H. and Leung,F.C.C.
  TITLE     Vertical and Horizontal Evolutionary Story implied from E. coli
            complete genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7618)
  AUTHORS   Jiang,J., Xu,Z., Zhou,Y., Zhao,H. and Leung,F.C.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2014) Bioinformatics Center, Nanjing Agricultural
            University, F214, South Wing, Science & Experiment Building, 1
            Weigang, Xuanwu District, Nanjing, Jiangsu 210095, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.7
            Genome Coverage                   :: 30-80x
            Sequencing Technology :: Illumina Miseq; Roche 454 GS Junior
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001901445.1-RS_2024_06_12
            Annotation Date                   :: 06/12/2024 04:26:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,878
            CDSs (total)                      :: 4,757
            Genes (coding)                    :: 4,587
            CDSs (with protein)               :: 4,587
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 170
            CDSs (without protein)            :: 170
            Pseudo Genes (ambiguous residues) :: 0 of 170
            Pseudo Genes (frameshifted)       :: 59 of 170
            Pseudo Genes (incomplete)         :: 114 of 170
            Pseudo Genes (internal stop)      :: 36 of 170
            Pseudo Genes (multiple problems)  :: 35 of 170
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP010245.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S56"
                     /isolation_source="forest soil"
                     /db_xref="taxon:562"
                     /plasmid="C"
                     /collection_date="2014"
     gene            complement(join(7535..7618,1..309))
                     /locus_tag="RG73_RS28150"
                     /old_locus_tag="RG73_24955"
     CDS             complement(join(7535..7618,1..309))
                     /locus_tag="RG73_RS28150"
                     /old_locus_tag="RG73_24955"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_249531204.1"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESG
                     HMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAEA
                     PAVYAGHPCRWRREPALPPDDLRADQ"
                     /name="MobA/MobL family protein"
     gene            508..792
                     /locus_tag="RG73_RS26365"
                     /old_locus_tag="RG73_24960"
     CDS             508..792
                     /locus_tag="RG73_RS26365"
                     /old_locus_tag="RG73_24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            complement(<1329..>1484)
                     /locus_tag="RG73_RS26370"
                     /pseudo=""
     CDS             complement(<1329..>1484)
                     /locus_tag="RG73_RS26370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017245253.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]; GO:0020037 - heme binding [Evidence IEA]"
                     /GO_process="GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /name="cbb3-type cytochrome c oxidase subunit I"
     gene            complement(<1497..1841)
                     /locus_tag="RG73_RS26375"
                     /old_locus_tag="RG73_24965"
                     /pseudo=""
     CDS             complement(<1497..1841)
                     /locus_tag="RG73_RS26375"
                     /old_locus_tag="RG73_24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019310991.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(1887..3227)
                     /locus_tag="RG73_RS26380"
                     /old_locus_tag="RG73_24970"
     CDS             complement(1887..3227)
                     /locus_tag="RG73_RS26380"
                     /old_locus_tag="RG73_24970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="WP_038989331.1"
                     /translation="MSILQFLIAVDVTVVNVALPSIGNDLGVDARGLTWVVVGYTITGG
                     GLLMLGGRLGDVFGRRRLLLIGTAIFGAASLMAGLAETFPLLVGARLLQGVGEALALPA
                     AMATIVLLFPEGRARTRALSVWAAVASCGLVLGFVLSGIITEYLGWRWIFLIAVPFVLF
                     VLIASVALLPRDRSQERAPLDILGSILLTSAPLLFAFGVVEAGDSNGDVPWSSIAALVG
                     AAAAAALFVKVERRAANPLVPLTFFRNRTRVRANLATALLSAALSTSFLLFTFYLQDGL
                     GLSPLESGLLLLPLAVALVVAVTVVPRLLGRWGARACIVVGLSSAGAGMIVIAAAAQLE
                     APAWLLTPAMLFIAAGMGFGLVGLQYAAVTEVTDDDAGVASGVQRAADQLGGSAGVTVY
                     VGLGFASALHGVSPYVLSSGLAIIGLCLATAIVQRIVIVTQEQQTRT"
                     /name="MFS transporter"
     gene            complement(3609..4460)
                     /gene="repC"
                     /locus_tag="RG73_RS26390"
                     /old_locus_tag="RG73_24975"
     CDS             complement(3609..4460)
                     /gene="repC"
                     /locus_tag="RG73_RS26390"
                     /old_locus_tag="RG73_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            complement(4447..5286)
                     /locus_tag="RG73_RS26395"
                     /old_locus_tag="RG73_24980"
     CDS             complement(4447..5286)
                     /locus_tag="RG73_RS26395"
                     /old_locus_tag="RG73_24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            complement(5316..5522)
                     /gene="urfF"
                     /locus_tag="RG73_RS26400"
                     /old_locus_tag="RG73_24985"
     CDS             complement(5316..5522)
                     /gene="urfF"
                     /locus_tag="RG73_RS26400"
                     /old_locus_tag="RG73_24985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     gene            complement(5524..5736)
                     /locus_tag="RG73_RS26405"
                     /old_locus_tag="RG73_24990"
     CDS             complement(5524..5736)
                     /locus_tag="RG73_RS26405"
                     /old_locus_tag="RG73_24990"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            complement(5797..6768)
                     /locus_tag="RG73_RS28155"
                     /old_locus_tag="RG73_24995"
     CDS             complement(5797..6768)
                     /locus_tag="RG73_RS28155"
                     /old_locus_tag="RG73_24995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_010890158.1"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            complement(6765..7178)
                     /locus_tag="RG73_RS28160"
     CDS             complement(6765..7178)
                     /locus_tag="RG73_RS28160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            complement(<7333..7557)
                     /locus_tag="RG73_RS28165"
                     /old_locus_tag="RG73_25000"
                     /pseudo=""
     CDS             complement(<7333..7557)
                     /locus_tag="RG73_RS28165"
                     /old_locus_tag="RG73_25000"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     Typing          complement(399..554)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          complement(4448..5269)
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(5801..7617)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctcggcaccg gtcaggtgct gggcgaactc ggacgccagc gccttctgct ggtcgagggt
       61 cagctcgacc ggcagggcaa attcgacctc cttgaacagc cgcccattgg cgcgttcata
      121 caggtcggca gcatcccagt agtcggcggg ccgctcgacg aactccggca tgtgcccgga
      181 ttcggcgtgc aagacttcat ccatgtcgcg ggcatacttg ccttcgcgct ggatgtagtc
      241 ggccttggcc ctggccgatt ggccgcccga cctgctgccg gttttcgccg taaggtgata
      301 aatcgccatg ctgcctcgct gttgcttttg cttttcggct ccatgcaatg gccctcggag
      361 agcgcaccgc ccgaagggtg gccgttaggc cagtttctcg aagagaaacc ggtaagtgcg
      421 ccctccccta caaagtaggg tcgggattgc cgccgctgtg cctccatgat agcctacgag
      481 acagcacatt aacaatgggg tgtcaagatg gttaagggga gcaacaaggc ggcggatcgg
      541 ctggccaagc tcgaagaaca acgagcgcga atcaatgccg aaattcagcg ggtgcgggca
      601 agggaacagc agcaagagcg caagaacgaa acaaggcgca aggtgctggt gggggccatg
      661 attttggcca aggtgaacag cagcgagtgg ccggaggatc ggctcatggc ggcaatggat
      721 gcgtaccttg aacgcgacca cgaccgcgcc ttgttcggtc tgccgccacg ccagaaggat
      781 gagccgggct gaatgatcga ccgagacagg ccctgcgggg ctgcacacgc gcccccaccc
      841 ttcgggtagg gggaaaggcc gctaaagcgg ctaaaagcgc tccagcgtat ttctgcgggg
      901 tttggtgtgg ggtttagcgg gctttgcccg cctttccccc tgccgcgcag cggtggggcg
      961 gtgtgtagcc tagcgcagcg aatagaccag ctatccggcc tctggccggg catattgggc
     1021 aagggcagca gcgccccaca agggcgctga taaccgcgcc tagtggatta ttcttagata
     1081 atcatggatg gatttttcca acaccccgcc agcccccgcc cctgctgggt ttgcaggttt
     1141 gggggcgtga cagttattgc aggggttcgt gacagttatt gcaggggggc gtgacagtta
     1201 ttgcaggggg cgtgacagtt attgcagggg ttcgtgacag ttagtacggg agtgacgggc
     1261 actggctggc gttgagcaga tcgttaccga cagttcagcg tcgcggcagt tacgcctcgt
     1321 gcagtggcgg gaaacgccag atcccgggtg ccgagcattt tgggtatgag atacatcgcc
     1381 agaccttcga gcaccggaat cgcaaataaa aacatcatca ccgtgccgtg cagggtaaag
     1441 acctggttgt acacatcggc acggagaatg tcctgtcccg gcaagggggt atattagaac
     1501 tcttgcgcgt cgagtcgatc ttcgaggtag gcggcgtact gccagcgcac ctggtcgagc
     1561 atgtagtcgg tgtcgagata gatcgtggtg acggtgatgt ggccctctgg ctccgatcct
     1621 gccagtacgt tcgcaccgag caagatcacg tctccgggtt tcaccgtctg ctgtccaaac
     1681 tcgctgaaca agaacgcaga gccgtcacgc accacgatca ccttgacgca gtcatacgcc
     1741 accggaccga gagggcggtg aatggtcgcg gtgcgcgcaa caaagggcgc gccccgcgac
     1801 caccggtcgg ggctgactga cgagtcgccg ggcgtcgcca cggagcacct catcgggaac
     1861 ttcgggatcg ggttacgacg gcacgctcat gtcctcgtct gttgttcctg cgtcacgatt
     1921 acgattcgtt ggacaatggc ggtcgcaagg cagaggccga tgatcgcgag gccgctactt
     1981 agcacatagg gtgatacacc gtgaagggcc gacgcgaagc ccaggcccac gtagaccgtg
     2041 acacctgcgg atccaccgag ctggtctgcg gctcgctgta cgccggatgc gactccggca
     2101 tcatcgtcgg tgacctcggt cacggccgcg tactgcaatc caacgaggcc gaaacccatt
     2161 cctgcggcga tgaagagcat cgcgggggtt agtagccaag cgggggcttc gagctgcgcg
     2221 gctgccgcga tcacgatcat gcctgcccct gcggaactca gtcccacgac gatgcaagca
     2281 cgcgctcccc agcgccccaa gaggcgaggt acgacggtca cggcgacgac tagtgccacc
     2341 gcaagtggca ggagcagcaa tccggattcc agcggagaga gaccgagtcc atcctgtaga
     2401 tagaacgtga acagcagaaa ggacgttgag agcgccgcgc tcaacagtgc agtcgcaaga
     2461 tttgctcgca ctcgtgtccg gttgcggaag aaggtcagcg gaaccagtgg gtttgcggct
     2521 cggcgttcca ccttgacgaa caatgcagcg gcggctgccg ctcccaccag ggcagcgatt
     2581 gatgaccagg gcacatcccc attgctgtcc ccggcctcca ccactccgaa agcgaacagc
     2641 agcggtgcgc tcgtcagcag aatcgatccg agaatatcca acggtgcgcg ctcctgtgat
     2701 cggtctcgag gtagcagggc gacagaagcg atcagcacga acaggacgaa cggaacggcg
     2761 atgaggaaga tccatcgcca accgagatac tcggtgatga ttcccgagag cacgaacccc
     2821 agcacaagac cgcagctcgc aaccgcagcc cagacgctca gggcgcgggt gcgcgcccgc
     2881 ccttcgggaa acaacaggac gatggtcgcc atcgcagcgg gcagggcgag ggcttcccct
     2941 acaccctgca acaaccttgc accgacgagt agcggaaagg tctccgcgag gcccgccatc
     3001 agcgaggccg ctccgaagat cgcggttccg atgagcagaa ggcggcggcg gccgaagacg
     3061 tctcccagac gtccaccaag catcaacaga ccaccgcctg tgatggtgta accaacgacg
     3121 acccatgtca ggccgcgcgc atcaacgccc agatcgttgc cgatagaggg cagcgccacg
     3181 ttgaccacgg tgacgtcgac ggcgatgagg aattgcagga tggacagaca aacaaggact
     3241 gcccaggaac gagcgaccga tacttgcgca ggccgcgatg aagaggtgct aaaagacaca
     3301 gagaactccg ttgctcgaat gaagatcggg catgagtatt atacggatgc tcgggtcgaa
     3361 agggaatccc caggcgagta acctgtttgc ggtgatccat tagctgcagg agcagaagag
     3421 catacatctg gaagcaaagc caggaaagcg gcctatggag ctgtgcggca gcgctcagta
     3481 ggcaattttt caaaatattg ttaagccttt tctgagcatg gtatttttca tggtattacc
     3541 aattagcagg aaaataagcc attgaatata aaagataaaa atgtcttgtt tacaatagag
     3601 tgggggggtc agcctgccgc cttgggccgg gtgatgtcgt acttgcccgc cgcgaactcg
     3661 gttaccgtcc agcccagcgc gaccagctcc ggcaacgcct cgcgcacccg ctggcggcgc
     3721 ttgcgcatgg tcgaaccact ggcctctgac ggccagacat agccgcacaa ggtatctatg
     3781 gaagccttgc cggttttgcc ggggtcgatc cagccacaca gccgctggtg cagcaggcgg
     3841 gcggtttcgc tgtccagcgc ccgcacctcg tccatgctga tgcgcacatg ctggccgcca
     3901 cccatgacgg cctgcgcgat caaggggttc agggccacgt acaggcgccc gtccgcctcg
     3961 tcgctggcgt actccgacag cagccgaaac ccctgccgct tgcggccatt ctgggcgatg
     4021 atggatacct tccaaaggcg ctcgatgcag tcctgtatgt gcttgagcgc cccaccacta
     4081 tcgacctctg ccccgatttc ctttgccagc gcccgatagc tacctttgac caccatggca
     4141 tcagcggtga cggcctccca cttgggttcc aggaacagcc ggagctgccg tccgccttcg
     4201 gtcttgggtt ccgggccaag cactaggcca ttaggcccag ccatggccac cagcccttgc
     4261 aggatgcgca gatcatcagc gcccagcggc tccgggccgc tgaactcgat ccgcttgccg
     4321 tcgccgtagt catacgtcac gtccagcttg ctgcgcttgc gctcgccccg cttgagggca
     4381 cggaacaggc cgggggccag acagtgcgcc gggtcgtgcc ggacgtggct gaggctgtgc
     4441 ttgttcttag gcttcaccac ggggcacccc cttgctcttg cgctgcctct ccagcacggc
     4501 gggcttgagc accccgccgt catgccgcct gaaccaccga tcagcgaacg gtgcgccata
     4561 gttggccttg ctcacaccga agcggacgaa gaaccggcgc tggtcgtcgt ccacacccca
     4621 ttcctcggcc tcggcgctgg tcatgctcga caggtaggac tgccagcgga tgttatcgac
     4681 cagtaccgag ctgccccggc tggcctgctg ctggtcgcct gcgcccatca tggccgcgcc
     4741 cttgctggca tggtgcagga acacgataga gcacccggta tcggcggcga tggcctccat
     4801 gcgaccgatg acctgggcca tggggccgct ggcgttttct tcctcgatgt ggaaccggcg
     4861 cagcgtgtcc agcaccatca ggcggcggcc ctcggcggcg cgcttgaggc cgtcgaacca
     4921 ctccggggcc atgatgttgg gcaggctgcc gatcagcggc tggatcagca ggccgtcagc
     4981 cacggcttgc cgttcctcgg cgctgaggtg cgccccaagg gcgtgcaggc ggtgatgaat
     5041 ggcggtgggc gggtcttcgg cgggcaggta gatcaccggg ccggtgggca gttcgcccac
     5101 ctccagcaga tccggcccgc ctgcaatctg tgcggccagt tgcagggcca gcatggattt
     5161 accggcacca ccgggcgaca ccagcgcccc gaccgtaccg gccaccatgt tgggcaaaac
     5221 gtagtccagc ggtggcggcg ctgctgcgaa cgcctccaga atattgatag gcttatgggt
     5281 agccattgat tgcctccttt gcaggcagtt ggtggttagg cgctggcggg gtcactaccc
     5341 ccgccctgcg ccgctctgag ttcttccagg cactcgcgca gcgcctcgta ttcgtcgtcg
     5401 gtcagccaga acttgcgctg acgcatccct ttggccttca tgcgctcggc atatcgcgct
     5461 tggcgtacag cgtcagggct ggccagcagg tcgccggtct gcttgtcctt ttggtctttc
     5521 atatcagtca ccgagaaact tgccggggcc gaaaggcttg tcttcgcgga acaaggacaa
     5581 ggtgcagccg tcaaggttaa ggctggccat atcagcgact gaaaagcggc cagcctcggc
     5641 cttgtttgac gtataaccaa agccaccggg caaccaatag cccttgtcac ttttgatcag
     5701 gtagaccgac cctgaagcgc ttttttcgta ttccataaaa cccccttctg tgcgtgagta
     5761 ctcatagtat aacaggcgtg agtaccaacg caagcactac atgctgaaat ctggcccgcc
     5821 cctgtccatg cctcgctggc ggggtgccgg tgcccgtgcc agctcggccc gcgcaagctg
     5881 gacgctgggc agacccatga ccttgctgac ggtgcgctcg atgtaatccg cttcgtggcc
     5941 gggcttgcgc tctgccagcg ctgggctggc ctcggccatg gccttgccga tttcctcggc
     6001 actgcggccc cggctggcca gcttctgcgc ggcgataaag tcgcacttgc tgaggtcatc
     6061 accgaagcgc ttgaccagcc cggccatctc gctgcggtac tcgtccagcg ccgtgcgccg
     6121 gtggcggcta agctgccgct cgggcagttc gaggctggcc agcctgcggg ccttctcctg
     6181 ctgccgctgg gcctgctcga tctgctggcc agcctgctgc accagcgccg ggccagcggt
     6241 ggcggtcttg cccttggatt cacgcagcag cacccacggc tgataaccgg cgcgggtggt
     6301 gtgcttgtcc ttgcggttgg tgaagcccgc caagcggcca tagtggcggc tgtcggcgct
     6361 ggccgggtcg gcgtcgtact cgctggccag cgtccgggca atctgccccc gaagttcacc
     6421 gcctgcggcg tcggccacct tgacccatgc ctgatagttc ttcgggctgg tttccactac
     6481 cagggcaggc tcccggccct cggctttcat gtcatccagg tcaaactcgc tgaggtcgtc
     6541 caccagcacc agaccatgcc gctcctgctc ggcgggcctg atatacacgt cattgccctg
     6601 ggcattcatc cgcttgagcc atggcgtgtt ctggagcact tcggcggctg accattcccg
     6661 gttcatcatc tggccggtgg tggcgtccct gacgccgata tcgaagcgct cacagcccat
     6721 ggccttgagc tgtcggccta tggcctgcaa agtcctgtcg ttcttcatcg ggccaccaag
     6781 cgcagccaga tcgagccgtc ctcggttgtc agtggcgtca ggtcgagcaa gagcaacgat
     6841 gcgatcagca gcaccaccgt aggcatcatg gaagccagca tcacggttag ccatagcttc
     6901 cagtgccacc cccgcgacgc gctccgggcg ctctgcgcgg cgctgctcac ctcggcggct
     6961 acctcccgca actctttggc cagctccacc catgccgccc ctgtctggcg ctgggctttc
     7021 agccactccg ccgcctgcgc ctcgctggcc tgctgggtct ggctcatgac ctgccgggct
     7081 tcgtcggcca gtgtcgccat gctctgggcc agcggttcga tctgctccgc taactcgttg
     7141 atgcctctgg atttcttcac tctgtcgatt gcgttcatgg tctattgcct cccggtattc
     7201 ctgtaagtcg atgatctggg cgttggcggt gtcgatgttc agggccacgt ctgcccggtc
     7261 ggtgcggatg ccccggcctt ccatctccac cacgttcggc cccaggtgaa caccgggcag
     7321 gcgctcgatg ccctgcgcct caagtgttct gtggtcaatg cgggcgtcgt ggccagcccg
     7381 ctctaatgcc cggttggcat ggtcggccca tgcctcgcgg gtctgctcaa gccatgcctt
     7441 gggcttgagc gcttcggtct tctgtgcccc gcccttctcc ggggtcttgc cgttgtaccg
     7501 cttgaaccac tgagcggcgg gccgctcgat gccgtcattg atccgctcgg agatcatcag
     7561 gtggcagtgc gggttctcgc cgccaccggc atggatggcc agcgtatacg gcaggcgc
//
