LOCUS       NZ_CP010175             6647 bp    DNA     circular CON 12-JUN-2024
DEFINITION  Escherichia coli strain H8 plasmid C, complete sequence.
ACCESSION   NZ_CP010175
VERSION     NZ_CP010175.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03252435
            Assembly: GCF_001900835.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Jiang,J., Xu,Z., Zhou,Y., Zhao,H. and Leung,F.C.C.
  TITLE     Vertical and Horizontal Evolutionary Story implied from E. coli
            complete genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Jiang,J., Xu,Z., Zhou,Y., Zhao,H. and Leung,F.C.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2014) Bioinformatics Center, Nanjing Agricultural
            University, F214, South Wing, Science & Experiment Building, 1
            Weigang, Xuanwu District, Nanjing, Jiangsu 210095, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.7
            Genome Coverage                   :: 30-80x
            Sequencing Technology :: Illumina Miseq; Roche 454 GS Junior
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001900835.1-RS_2024_06_12
            Annotation Date                   :: 06/12/2024 04:38:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,010
            CDSs (total)                      :: 4,888
            Genes (coding)                    :: 4,640
            CDSs (with protein)               :: 4,640
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 248
            CDSs (without protein)            :: 248
            Pseudo Genes (ambiguous residues) :: 0 of 248
            Pseudo Genes (frameshifted)       :: 96 of 248
            Pseudo Genes (incomplete)         :: 170 of 248
            Pseudo Genes (internal stop)      :: 56 of 248
            Pseudo Genes (multiple problems)  :: 67 of 248
            Pseudo Genes (short protein)      :: 1 of 248
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP010175.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="H8"
                     /isolation_source="river water"
                     /db_xref="taxon:562"
                     /plasmid="C"
                     /collection_date="2014"
     gene            476..703
                     /locus_tag="RG50_RS27260"
                     /old_locus_tag="RG50_25550"
     CDS             476..703
                     /locus_tag="RG50_RS27260"
                     /old_locus_tag="RG50_25550"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(700..2268)
                     /gene="cea"
                     /locus_tag="RG50_RS27265"
                     /old_locus_tag="RG50_25555"
     CDS             complement(700..2268)
                     /gene="cea"
                     /locus_tag="RG50_RS27265"
                     /old_locus_tag="RG50_25555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            2528..2965
                     /locus_tag="RG50_RS27270"
                     /old_locus_tag="RG50_25560"
     CDS             2528..2965
                     /locus_tag="RG50_RS27270"
                     /old_locus_tag="RG50_25560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            3046..3465
                     /locus_tag="RG50_RS27275"
                     /old_locus_tag="RG50_25565"
     CDS             3046..3465
                     /locus_tag="RG50_RS27275"
                     /old_locus_tag="RG50_25565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(3783..4016)
                     /locus_tag="RG50_RS28945"
     CDS             complement(3783..4016)
                     /locus_tag="RG50_RS28945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4023..4541)
                     /locus_tag="RG50_RS28950"
     CDS             complement(4023..4541)
                     /locus_tag="RG50_RS28950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4579..5277)
                     /locus_tag="RG50_RS29750"
                     /pseudo=""
     CDS             complement(<4579..5277)
                     /locus_tag="RG50_RS29750"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5219..5542)
                     /locus_tag="RG50_RS27295"
                     /old_locus_tag="RG50_25580"
     CDS             complement(5219..5542)
                     /locus_tag="RG50_RS27295"
                     /old_locus_tag="RG50_25580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5606..5797
                     /locus_tag="RG50_RS27300"
                     /old_locus_tag="RG50_25585"
     CDS             5606..5797
                     /locus_tag="RG50_RS27300"
                     /old_locus_tag="RG50_25585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6467..6625
                     /locus_tag="RG50_RS29755"
     CDS             6467..6625
                     /locus_tag="RG50_RS29755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     Typing          complement(5883..5970)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          5981..6539
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.544"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3680..5229)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
       61 agttggtagc tcttgatccg gcaaacaaac caccgttggt agcggtggtt tttttgtttg
      121 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttaa tcttttctac
      181 tgaaccgcga tccccgtcag tttagaagag gaggatggtg cgatggtccc tccctgaaca
      241 tcacgtatat agttagcctg acatccaaca aggaggttta tcgcgaatat tcccacaaaa
      301 aatcttttcc tcataactcg atccttataa aatgaaaaga atatatggcg aggtttaatt
      361 tatgagctta agatactaca taaaaaatat tttatttggc ctgtactgca cacttatata
      421 tatatacctt ataacaaaaa acagcgaagg gtattatttc cttgtgtcag ataagatgct
      481 atatgcaata gtgataagca ctattctatg tccatattca aaatatgcta ttgaatacat
      541 agcttttaac ttcataaaga aagatttttt cgaaagaaga aaaaacctaa ataacgcccc
      601 cgtagcaaaa ttaaacctat ttatgctata taatctactt tgtttggtcc tagcaatccc
      661 atttggattg ctaggacttt ttatatcaat aaagaataat taaatcccta acacctcatt
      721 tatagtatta agtttattct tatcaatata ggagcataga attcctgtaa caatagcaat
      781 accccaaata cctaatgtag ttccagcaag caagctaaaa agtaaagcaa caacataact
      841 cacccctgca tctgcagctt tcttctctaa tgtaagaaat agtggcttcc agtcacctgt
      901 atccttaatt tttaggatat cagataccac atcatatcca aaagaaacat gccccgtaat
      961 cttcaagtac ttggcaaact gatctaaatg tttagcccag tcatcatact tcaccgatgc
     1021 caacgcatta aaaatagcat cacgatcggc tttgctgaat ttcttattta aaacatcctt
     1081 gtatttttca aaagcagcga gagcttcatt cacattgccg attttcttac ctttagactt
     1141 atcagcaagt tcctgtgcca ttttcgaata tttttcacca tatttttcag tcagcgtttg
     1201 ataaaagcta actgttgcat caacagcatc cttaatctgt gaattaagga gattattctg
     1261 tgcttttttc aaattttctt cagcttcatg aacacgagcg ataccggcat tacgattatt
     1321 actgacctga gaaatagcct tctggatctg agttatatca gcatttatcc ggttaatacg
     1381 tgtttctgat gctgttacct gtttttgttt ttcttctcta atcttaccgg ccccaacccg
     1441 tcgtctggtt gcttcaaaaa aaggacggtt ctgaagcgga tcattggctc ttggtgatag
     1501 ttttttgacc agctcatcca gttctttata tttagcggat gcctgagcca gttcatttcg
     1561 ttttccagcg agcgttttca tttctgcatc acgggcatgg atactggagc ttaaacgaga
     1621 attgagagtc ttaatctctc catccatttt caccacttca gattgtgcag cagaaagttt
     1681 tttttgggcg atctcaacag ctttagcttc ttcactcaat gcagccagtc gtttctcttc
     1741 agcttcagcc agtttcaact ggcgttctgt ttcagccttc tcccgttcaa tctctttacg
     1801 tcgttgttct gcttcctgaa aagccttttc tgctgcttcc gcttctttac gggctttttc
     1861 ttctgctttc gcaaggcgca aacgctctgc ttccgcctgc atagctgcat tattagcatg
     1921 agcaagctct gttgctgaag gcgtacgtga ggcattgtga cgaagagcct cattcacgat
     1981 atccttcagg cgctgagtca gcgcatccct gtttgccttt gctttcgcct gtgcttccgc
     2041 tgcagctttt gcccgggcag cctgctctgc ctgtgttttc tttaattgag cagtagacca
     2101 tttagcagtt gcatgaatag ctgcagaact ttcactttta ctgcctcctt ttccacctcc
     2161 gccgccagag ccactcccgt caggagtacc attcaaaaga gtaataatta cctgtccctt
     2221 atcatcataa ggaacaccat ctttatagta cgctaccgcg gtttccatta taaaatcctc
     2281 tttgactttt aaaacaataa gttaaaaata aatactgtac atataaccac tggttttata
     2341 tacagcataa aagctacgcc gctgcatttt ccctgtcaag actgtggact tccatttttg
     2401 tgaaaacgat caaaaaaaca gtctttcaca ccacgcgcta ttctcgcccg atgccacaaa
     2461 aaccagcaca aacattaccg ttctcagacc tcattatgtt ttactgaaac tatgagatga
     2521 gacatctatg ggacactgtc actttatggc atggcacaca ctccgggacg cactaaaaat
     2581 gacaggcaga tcgcgttcac agttttaccg tgatatgcgc gcaggccttg tcagttaccg
     2641 taccggcagg gacggacgac gggagtttga aaccagtgaa ctgatccggg catacggcga
     2701 attaaagcag aatgagacac cagaaaggca cagtgaggga catgcagaaa atccacatga
     2761 tcagcagaca gaacgcattc tccgggaact gaatgagctg aaacaatgcc tgacgctgat
     2821 gcttgaggat aaacaggcac aggatatgga tcgcagacgc caggaagcag aacgggaaca
     2881 gctacaaaat gagatagccc agctcaggca ggcactggaa ctggaaaaga aacggggatt
     2941 ctggtccagg ttgttcggtc gctgaacgct gtcagagact gatgataaaa tagtcttcgg
     3001 ataataactc accgagaata aatactttaa ggtagggaga cactcatgag acgtaccgga
     3061 aacaaacttt gtcttatcgc catgataaca gcaacagtag ctctcacagc ctgtacccca
     3121 aagggcagcg tggaacaaca tacccggcat tacgtatatg cttctgatga cggttttgat
     3181 cccaactttt ccacccaaaa agccgacaca acacgaatga tggtgccttt ttttcggcag
     3241 ttctgggata tgggagctaa agataaagcg acaggaaaat cacggagtga tgtgcaacaa
     3301 cgcattcagc agtttcacag ccaggaattt ttaaactcac tccggggcac aactcaattt
     3361 gcgggtactg attaccgcag caaagacctt accccgaaaa aatccaggct gctggctgac
     3421 acgatttctg cggtttatct cgatggctac gagggcagac agtaagtgga tttaccataa
     3481 tcccttaatt gtacgcaccg ctaaaacgcg ttcagcgcga tcacggcagc agacaggtaa
     3541 aaatggcaac aaaccacccg aaaaactgcc gcgatcgcgc ctgataaatt ttaaccgtat
     3601 gaatacctat gcaaccagag ggtacaggcc acattacccc cacttaatcc actgaagctg
     3661 ccatttttca tggtttcacc atcccagcga agggccatcc agcgtgcgtt cctgtatttc
     3721 cggctgacgc tcccgttcca gggataacac atgttcgcgc tcctgtatca gccgttcctc
     3781 tcttatctcc agttctcgct gtataactgg ctcaagcgtt ctgtctgctc gctcaagtgc
     3841 tgcacctgct gactcaactg catgacccgc tcgttcagca tcgcgttgtc ccgttgcgta
     3901 agcgaaaaca tcttctgcaa ttccacgaag gcgctctccc attcgctcag ccgctgcata
     3961 tagtcctgtt gcagctgctc taaggcgttc agcaaatgtg tttccagctc tgtcactctg
     4021 tgtcactcct tcagatgtac ccactctttc ccctgaaagg gaatcacctc cgctgatttc
     4081 ccgtacggaa ggacaaggaa tttcctgttc ccgtcctgca caaactccac gccccatgtc
     4141 ttcgcgttca gtttctgcaa tgtctcttcc tgcttcctga tttcttccag gttcgcctgt
     4201 atcctccctc caagatacca gagcgtcccg ccactcgcgg taaacaggag aaagactatc
     4261 cccagtaaca tcatgcccgt attccctgcc agctttaaca cgtccctcct gtgctgcatc
     4321 atcgcctctt tcaccccttc ccggtgtttt tccagcgatt cctctgtcga ggctgtgaac
     4381 agggctatag cgtctctgat tttcgtctcg tttgatgtca cagcctcgct tacagattcg
     4441 ccgagcctcc tgaactcgtt gttcagcatt ttctctgtag attcggctct ctctttcagc
     4501 tttttctcga actccgcgcc cgtctgcaaa agattgctca taaaatgctc ctttcagcct
     4561 gatattcttc ccgccgttcg gatctgcaat gctgatactg cttcgcgtca ccctgaccac
     4621 ttccagcccc gcctcagtga gcgcctgaat cacatcctga cggcctttta tctctccggc
     4681 atggtaaagt gcatctatac ctcgcgtgac gccctcagca agcgcctgtt tcgtttcagg
     4741 caggttatca gggagtgtca gcgtcctgcg gttctccggg gcgttcgggt catgcagccc
     4801 gtaatggtga tttaccagcg tctgccaggc atcaattcta ggcctgtctg cgcggtcgta
     4861 gtacggctgg aggcgttttc cggtctgtag ctccatgttc ggaatgacaa aattcagctc
     4921 aagccgtccc ttgtcctggt gctccaccca caggatgctg tactgatttt tttcgagacc
     4981 gggcatcagt acacgctcaa agctcgccat cactttttca cgtcctcccg gcggcagctc
     5041 cttctccgcg aacgacagaa caccggacgt gtatttcttc gcaaatggcg tggcatcgat
     5101 gagttcccgg acttcttccg gattaccctg aagcaccgtt gcgccttcgc ggttacgctc
     5161 cctccccagc aggtaatcaa ccggaccact gccaccacct tttcccctgg catgaaattt
     5221 aactatcatc ccgcgccccc tgttccctga cagccagacg cagccggcgc agctcatccc
     5281 cgatggccat cagtgcggcc accacctgaa cccggtcacc ggaagaccac tgcccgctgt
     5341 tcaccttacg ggctgtctga ttcaggttat ttccgatggc ggccagctga cgcagtaacg
     5401 gcggtgccag tgtcggcagt tttccggaac gggcaaccgg ctcccccagg cagacccgcc
     5461 gcatccatac cgccagttgt ttaccctcac agcgttcaag taaccgggca tgttcatcat
     5521 cagtaacccg tattgtgagc atcctctcgc gtttcatcgg tatcattacc ccatgaacag
     5581 aaatccccct tacacggagg catcagtgac taaacaggaa aaaaccgccc ttaacatggc
     5641 ccgctttatc agaagccaga cattaacgct gctggagaag ctcaacgaac tggacgcaga
     5701 tgaacaggcc gatatttgtg aatcgcttca cgaccacgcc gatgagcttt accgcagctg
     5761 cctcgcacgt ttcggggatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     5821 cacagcttgt ctgtgagcgg atgccgggag ctgacaagcc cgtcagggcg cgtcagcagg
     5881 ttttagcggg tgtcggggcg cagccctgac ccagtcacgt agcgatagcg gagtgtatac
     5941 tggcttaacc atgcggcatc agtgcggatt gtatgaaaag tacgccatgc cgggtgtgaa
     6001 atgccgcaca gatgcgtaag gagaaaatgc acgtccaggc gcttttccgc ttcctcgctc
     6061 actgactcgc tacgctcggt cgttcgactg cggcgagcgg tactgactca cacaaaaacg
     6121 gtaacacagt tatccacaga atcaggggat aaggccggaa agaacatgtg agcaaaagac
     6181 caggaacagg aagaaggcca cgtagcaggc gtttttccat aggctccgcc cccctgacga
     6241 gcatcacaaa aatagacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6301 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6361 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6421 ttggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6481 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6541 gcacgcctta acgccactgg cagcagccac tggtaaccgg attagcagag cgatgatggc
     6601 acaaacggtg ctacagagtt cttgaagtag tggcccgact acggcta
//
