LOCUS       NZ_CP010128             7982 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain C8 plasmid C, complete sequence.
ACCESSION   NZ_CP010128
VERSION     NZ_CP010128.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03252415
            Assembly: GCF_001900555.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Jiang,J., Xu,Z., Zhou,Y., Zhao,H. and Leung,F.C.C.
  TITLE     Vertical and Horizontal Evolutionary Story implied from E. coli
            complete genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7982)
  AUTHORS   Jiang,J., Xu,Z., Zhou,Y., Zhao,H. and Leung,F.C.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2014) Bioinformatics Center, Nanjing Agricultural
            University, F214, South Wing, Science & Experiment Building, 1
            Weigang, Xuanwu District, Nanjing, Jiangsu 210095, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.7
            Genome Coverage                   :: 30-80x
            Sequencing Technology :: Illumina Miseq; Roche 454 GS Junior
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001900555.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 03:10:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,830
            Genes (coding)                    :: 4,653
            CDSs (with protein)               :: 4,653
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 8, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 78 of 177
            Pseudo Genes (incomplete)         :: 91 of 177
            Pseudo Genes (internal stop)      :: 51 of 177
            Pseudo Genes (multiple problems)  :: 36 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP010128.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="C8"
                     /host="cow"
                     /db_xref="taxon:562"
                     /plasmid="C"
                     /collection_date="2014"
     gene            complement(join(7741..7982,1..289))
                     /locus_tag="RG30_RS28420"
     CDS             complement(join(7741..7982,1..289))
                     /locus_tag="RG30_RS28420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_073534392.1"
                     /translation="MSNLLQTGAEFEKKLKERAASTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVSTMDTHWQTVLKMSVKRWLWLIIVSVLMFTTTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTE
                     DKGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<327..1025)
                     /locus_tag="RG30_RS28425"
                     /old_locus_tag="RG30_25200"
                     /pseudo=""
     CDS             complement(<327..1025)
                     /locus_tag="RG30_RS28425"
                     /old_locus_tag="RG30_25200"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(967..1290)
                     /locus_tag="RG30_RS26540"
                     /old_locus_tag="RG30_25205"
     CDS             complement(967..1290)
                     /locus_tag="RG30_RS26540"
                     /old_locus_tag="RG30_25205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956000.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1355..1546
                     /locus_tag="RG30_RS26545"
                     /old_locus_tag="RG30_25210"
     CDS             1355..1546
                     /locus_tag="RG30_RS26545"
                     /old_locus_tag="RG30_25210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_096939302.1"
                     /translation="MTKQEKTTLNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENH"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2216..2374
                     /locus_tag="RG30_RS27800"
     CDS             2216..2374
                     /locus_tag="RG30_RS27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001354639.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAAVGNLVSRAMMA
                     QMVQQSS"
                     /name="hypothetical protein"
     gene            2870..3097
                     /locus_tag="RG30_RS26565"
                     /old_locus_tag="RG30_25215"
     CDS             2870..3097
                     /locus_tag="RG30_RS26565"
                     /old_locus_tag="RG30_25215"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_247172693.1"
                     /translation="MLYAIVISTILCPYSKYAIEHIFFKFIKKGFFRKRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(3094..4659)
                     /locus_tag="RG30_RS26570"
                     /old_locus_tag="RG30_25220"
     CDS             complement(3094..4659)
                     /locus_tag="RG30_RS26570"
                     /old_locus_tag="RG30_25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_072643317.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAAQAKAAVEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEAEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEIKTLNTRLSSSI
                     HARDAEMNTLSGKRNELAQASAKYKELDELVKKLSPRANDPLQSRPFFYATSRRTGAGK
                     IMEEKQKQVTASETRINQLNAEINGIQGAMSQANNNRNTAVLRVHESEENLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALESVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKTVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTIND
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            4936..5355
                     /locus_tag="RG30_RS26575"
                     /old_locus_tag="RG30_25225"
     CDS             4936..5355
                     /locus_tag="RG30_RS26575"
                     /old_locus_tag="RG30_25225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312318.1"
                     /translation="MAWHTLQDALKMTGRSRSQFYRDMRAGLVSYRTGQDGRREFETSE
                     LIRAYGELKQDETAERHSAGQAENPHDQQTERILRELNELKQCLTLMLEDKQAQDTERR
                     RQEAEREQLHKEIEQLRQALEQEKKRGFWARLFSR"
                     /name="hypothetical protein"
     gene            5439..5858
                     /locus_tag="RG30_RS26580"
                     /old_locus_tag="RG30_25230"
     CDS             5439..5858
                     /locus_tag="RG30_RS26580"
                     /old_locus_tag="RG30_25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254598.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(7501..7734)
                     /locus_tag="RG30_RS26595"
                     /old_locus_tag="RG30_25245"
     CDS             complement(7501..7734)
                     /locus_tag="RG30_RS26595"
                     /old_locus_tag="RG30_25245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_073534391.1"
                     /translation="MTELEKHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRGNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1632..1719)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          1742..2288
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.384"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(4..977)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.538"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(7419..7978)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.268"
                     /coverage="99.0"
                     /evalue="4.67e-87"
ORIGIN
        1 ctgagacgat aatcagccac agccagcgtt tcacgctcat tttcagcacg gtttgccagt
       61 gtgtgtccat cgtactgacg gtcgttttct gatacctctc cagaagttct ttcagggctg
      121 ttgtatggtc gttgatagcg ttcctgatta aagattcgtt tgaggtcagt tccctgttta
      181 cagattcttc gagcttcctg aactcgtcgt tcagcatttt ctctgtagat gcggctctct
      241 ctttcagctt tttctcgaac tccgcgcccg tctgtaaaag attgctcata gaatgctcct
      301 ttcagcctga tattcttccc gccgttcggg tctgcaatgc tgatgctgct tcgcgtcact
      361 ctgaccactt caagccccgc ttccgtgagc gcctgaatca catcctgacg accttttatc
      421 tctccggcat ggtaaagtgc atctatacct cgtgtgacgc cctcagcaag cgcctgtttc
      481 gtttccggca ggttatcagg gagagtcagt atccggcggt tctccggggc gttggggtca
      541 tgcagcccgt aatggtgatt aaccagcgtc tgccaggcat caattctcgg cctgtctgcc
      601 cggtcgtagt acggctggag gcgttttccg gtctgtaact ccatgttcgg aatgacaaaa
      661 ttcagctcaa gccgtccctt gtcctggtgc tctacccaca ggatgctgta ctgattcttt
      721 tcgagaccgg gcatcagtac acgctcaaag ctcgccatca ctttttcacg ccctcccggc
      781 ggcagctcct tctccgcgaa cgacagaaca cctgacgtgt atttcttcgc aaatggcgtg
      841 gcatcgatga gttcccggac ttcttccgga ttaccctgaa gcaccgttgc cccttcccgg
      901 ttacgctccc ttcccagcag gtaatcaacc ggaccactgc caccaccttt tcccctggca
      961 tgaaatttaa ctatcatccc gcgtcccctg ttccctgacg gccagacgca gccggcgcag
     1021 ctcatccccg atggccatca gtgcggccac tacctgaacc cggtcaccgg aagaccactg
     1081 cccgctgttc accttcctcg ctgtctgatt caggttattc ccgatggcgg ccagctgacg
     1141 cagtaacggc ggtgccagtg taggcagctt cccggaacgg gcaaccggct cacccaggca
     1201 gacccgccgc atccataccg ccagttgttt accctcacag cgttccagta accgggcatg
     1261 ttcatcatca gtaacccgta tcgtgagcat cctctcgcgt ttcatcggta tcattacccc
     1321 catgaacaga aatccccctt acacggaggc atcagtgact aaacaggaaa aaaccaccct
     1381 taacatggcc cgcttcatca gaggccagac attaacgctg ctggagaagc tcaacgaact
     1441 ggacgcggat gaacaggcag atatctgtga atcgcttcac gaccacgccg atgagcttta
     1501 ccgcagctgc ctcgcacgct tcggggatga cggtgaaaac cactgacaca tgcagctccc
     1561 ggagacggtc acagcttgtc tgtgagcgga tgccgggagc agacaagccc gtcagggcgc
     1621 gtcagcaggt tttagcgggt gtcggggcgc agccctgacc cagtcacgta gcgatagcgg
     1681 agtgtatact ggcttagcca tgcggcatca gtgcggattg tatgaaaagt gcaccatgta
     1741 cggtgtgaaa tgccgcacag atgcgtaagg cgaaaatgca ggtaccggtg ctcttccgct
     1801 tcctcgctca ctgactcgct acgctcggtc gttcggctgc ggcgagcggt gtctgctcac
     1861 tcaaaagcgg cgatactgtt atccacagaa tcaggggata aagccggaaa gaacatgtga
     1921 gcaaaaaagc aaaaccaggg aaaaggccgc gccgcaggcg tttttccata ggctccgccc
     1981 ccctgacgag catcacaaaa acagacgctc aagtcagagg tggcgaaacc cgacaggact
     2041 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2101 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2161 ctcacgctgt tggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2221 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2281 cccggtaaga cacgacaaag cgccactggc agcagccgtt ggtaacttgg ttagcagagc
     2341 gatgatggcg caaatggtgc aacagagttc ttgaagtagt ggcccgacta cggctacact
     2401 ggaaggacag tatttggtat ctgcgctctg ctgaagccag ttacctcggt taagcagttc
     2461 cccaactgac ttaaccttcg atcaaaccgc ctccccaggc ggttttttcg tttacaggca
     2521 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctactgaaca
     2581 gagatccccg tcaatttaga agaagatgat ggagccacag tccctccctg aacatcacgt
     2641 atatagttag cctgacatcc aacaaggagg tttatagcta atattcccac aaaaaatctt
     2701 ttcctcataa ctcgatcctt ataaaatgaa aagaatatat aacgaggttt aatttatgag
     2761 tttaagatac tacataaaaa acattttgtt tggcctatat tgcacactta tatatatata
     2821 ccttataaca aaaaataacg aagggcatta tttcctagcg tcagataaga tgctatacgc
     2881 aatagtgata agcactattc tatgcccata ttcaaaatat gctattgaac acatattttt
     2941 taagttcata aagaaaggtt ttttcagaaa aagaaaaaat ctaaataatg cccccgtggc
     3001 aaaattaaac ctatttatgc tatacaatct actttgtttg gtcctagcaa tcccatttgg
     3061 attgctagga ctgtttatat caataaagaa taattaaata cccaacacat catttatagt
     3121 attaagttta ttcttgtcaa taaaggcaca tagaatgcct gtaacaatag caatccccca
     3181 gatacctaat gtagttccag caagcacact aaaaagtaaa acaacaacat aactaactcc
     3241 agcatctaca gttttcttct ctaatgtaag aaatagtggc ttccagtcac ctgtatcctt
     3301 aatttttagg atatcagata ccacatcata tccaaaagaa acatgtcccg taatcttcaa
     3361 gtacttagca aactgatcta aatgcttagc ccagtcttca tacttaaccg attccagtgc
     3421 attgaaaatc gcatcacggt ctgctttgct gaatttctta tttaaaacat ccttgtattt
     3481 ttcaaaagca gctagagctt cattcacatt gctgattttc ttacctttag acttatcagc
     3541 aagctcctgt gccatttttg aatatttttc accatatttt tcagatagcg tttgataaaa
     3601 gctaactgtt gcatccacag catccttaat ctgcgagttc aggagattag tctgcgctgt
     3661 tttcaaattt tcttcagatt catgaacacg taaaacagct gtattacgat tattattagc
     3721 ctgagacata gccccctgaa ttccatttat ctcagcatta agttggttaa tacgcgtttc
     3781 tgatgctgtt acctgttttt gtttttcctc cataatctta ccggctcccg tccgtcgact
     3841 ggtggcatag aaaaaaggac gactctgaag cggatcatta gctcttggtg acaatttttt
     3901 gaccagttca tcaagttcct tatatttagc agatgcctga gccagctcat ttcgttttcc
     3961 ggacagcgta ttcatttctg catcacgggc atgaatactg gagcttaaac gggtattgag
     4021 agtcttaatc tcttcatcca ctttcgccag ctcagattgt gcagcagcaa gatttttttg
     4081 tgccacctcc actgcccggg cctcttcact caatgctgcc agccgtttct cttcagcttc
     4141 agccagtttc aactggcgtt ctgtttcagc ctgctccttc tctatctctt tacgtcgttg
     4201 ttctgcctcc tgaaaagcct tttccgcagc ttccgcttct ttacgggctt tttcttctgc
     4261 ttttgcaaga cgcaaccgct ctgcttctgc ctgcatcgct gcattattgg catgagccag
     4321 gtcaataacc tccggatgag tggaattatg acgaagcgcc tcattcacaa tatccttcag
     4381 atgttgagtc agcgcatccc ggtttgcctt tgctttagcc tgtgcttcta cggcagcttt
     4441 tgcctgggca gcctgttctg cctgcgtttt cttcaattga gcagtagacc atttagctgt
     4501 ggcatgaatg gctgcagaac tttcactttt gctacctcca gttccaccac cgccgccaga
     4561 gccactcccg tctggattac cattcaaaag agtaatgatt acctgcccct tatcatcata
     4621 aggaacacca tctttatagt acgctacagc tgtttccatt ataaaatcct ctttgacatt
     4681 aaaaacaaat agttaaaagc aatatactgt atatataacc actggtttta tatacagttt
     4741 aaaattacgc cgctgcattt tccctgtcaa ccctgtggat tttcattttt gtgaaaacgt
     4801 tcaaaaaaaa gccacccaca ccacgtgcta ttcccacccg atgccacaaa aaccagcaca
     4861 aacattaccg ttctccggcc tcagtatgtt ttactgaaac catgatatga gacaccaatg
     4921 ggacactgtc acttcatggc atggcacaca ctccaggacg cactgaaaat gacaggcaga
     4981 tcgcgttcac agttttaccg tgatatgcgc gcaggccttg tcagttaccg taccggccag
     5041 gacggacgac gggagtttga aaccagtgaa ctgatccggg catacggcga attaaagcag
     5101 gatgagacag cagaaaggca cagtgcggga caggcagaaa atccacatga tcagcagaca
     5161 gaacgcattc tccgggagct gaatgagctg aaacaatgcc tgacactgat gcttgaggac
     5221 aaacaggcac aggatacgga acgccggcgc caagaagccg aacgggaaca gctacataaa
     5281 gagatagagc agctcaggca ggcacttgag caggaaaaaa aacggggatt ctgggccaga
     5341 ctgttcagcc ggtgagcacc ggctgcaagt gatgataaga tagtcagcgg ttaaatataa
     5401 gtcactgagt acaaagattt aaaggtaagg agacactcat gagacgttcc ggcggcacac
     5461 tgtatcttat cgccctgctg acagcggcaa cggtcctgac agcctgcacg ccaaagggca
     5521 gtgtggaaca acatacccgg cattacgttt atgcatcaga cgatggtttt gatcccaact
     5581 tttccaccca gaaggccgac acaacacgaa tgatggtgcc ttttttccgg cagttctggg
     5641 atatgggagc aaaggataaa gcgacaggaa aatcacggag tgatgtgcag caacgcattc
     5701 agcagtttca cagccaggaa tttttaaact cactccgggg cacaactcaa tttgcgggta
     5761 ctgattaccg cagcaaagac cttaccccga aaaaatccag gctgctggct gagacgattt
     5821 ctgcggttta tctcgatggc tacgagggaa gacagtaagt ggatttacca taatccctta
     5881 attgtacgca ccactgaaat gcgttcagcg cgatctcggc agcagacagg taaaaatggc
     5941 aacaaaccac ccgaaaaacc gccgcgatcg cgcctgataa attttaatct tatgcatatc
     6001 tatgcagcca ggcgaatcac gaacgaatta cctgcctgag attacttaaa cgggtgtttt
     6061 ttcatatttt cagccggatc aattcctgcc agatggaagc atctgggggg gggaatctta
     6121 ctgtgcgtga cagaactcat caccgtacac acactgtata aggatccaga aaaaggaaac
     6181 aattgcctga taccagaaat cgcgttacaa cacttctgag cgcgtttctg acagtctcca
     6241 tcaactgagc tgaatgttcg tcacacaaca tcgaaacgtg ctcaccatcc gcgaccaacg
     6301 tccaatgaaa ctctctcttc cggaagcgcc caattcgaaa aataaccggt agacctcctg
     6361 tctgtctcag aattctggca cagtaatcag ggaccataac tttacagcgc cccctggcct
     6421 gcatcgaaac taaaaaccgc cggaagaagt gttttaaaat ggtgaaccag acatccacac
     6481 ctctcgacac agagcccttg ctcagctaaa aatttacagg cgcgtttctg gtgaattgtt
     6541 cgttgatgta gaatgggtta gtcgcatcac cgccagaaat gcgctcataa gacagaactt
     6601 cactgccttt atagttacta ctcaaaacag tataaaacag agagaaaaag ataaaaaaaa
     6661 gtccctcaag gagcagggac tgttaattgc gtctgaatat aagaaaaaat tagaagaatt
     6721 cactttcagc ttaagaataa cggcaccgag caacaacgat caacaaaaat gattagattt
     6781 tattttctgt tattttgtct tttttgtaaa aaacaaaaca attaacccag aaaattcctg
     6841 gcatcaatat tcttgtttat agttcgcttt atggttaatc ttattactct ccagaggtga
     6901 ccaacaccag gatatttttt tgctctgtat cactcagccc ctccgtcagg tggggcttcc
     6961 ttttttcaca cccatgaaaa aagccctcac ggcaggaact tagtgagggc gtaaggtaag
     7021 aactgaatag gtggatagta tgtctagtcc tgacttttgc atatcagaat ataatttgca
     7081 tgtttccaag atgcaaagaa aaaataaaaa atattcacat cacaatataa gaacacaata
     7141 ttaacacatg agagaacgcg catctccgac cccttccaga ttcgctgtgt ccacatcggt
     7201 attacaaaca tatcggagat acgcaattat attttttaca taatcagcgc attcccgcaa
     7261 gttcattttc agaaaaatcc ctgtcctctt tacaaaaaaa acagcaacaa cttgtacaaa
     7321 aatcaataaa ccgcaagaac gactggttat attaccaata ggcagcctga catttctgga
     7381 aaatattctg tttacgactc aaccggactt tcagtcacca tcccagcgaa gggccatcca
     7441 gcgtccgctc ccgttccaga gataactcat gttcgcgttc ctgcatcagc cgttcatccc
     7501 ttatctcctg ttctcgctgt ataactggct caagcgctct gtccgctcgc tcaagtgctg
     7561 cacctgctga ctcaactgca tgacccgctc gttcagcatc gcgttgcccc gttgcgtaag
     7621 cgaaaacatt ttctgcaatt ccacgaaggc gctctcccat tcgttcagcc gctgcatata
     7681 gtcctgttgc agttgctcta atgcgttcag caaatgtttt tccagctccg tcatgctctg
     7741 tcactcctgt accagtctca ctgcgttcag tttgcctttg tcctccgtcc agttcgtttc
     7801 ggctttcatc cccttcggca acaccagaaa acgtccgttg ctgtcctcgt gataacgcac
     7861 gccccatgtc ttcgcgttca gcttctccag actctctttc tgctcgcgga gaatattcat
     7921 gtttgcgttt atcttcatgc cctggtacca cagcagactc ccggtcgtgg taaacatcag
     7981 ca
//
