LOCUS       NZ_CP010118             8401 bp    DNA     circular CON 13-JUN-2024
DEFINITION  Escherichia coli strain C2 plasmid A, complete sequence.
ACCESSION   NZ_CP010118
VERSION     NZ_CP010118.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03252410
            Assembly: GCF_001900495.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Jiang,J., Xu,Z., Zhou,Y., Zhao,H. and Leung,F.C.C.
  TITLE     Vertical and Horizontal Evolutionary Story implied from E. coli
            complete genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Jiang,J., Xu,Z., Zhou,Y., Zhao,H. and Leung,F.C.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2014) Bioinformatics Center, Nanjing Agricultural
            University, F214, South Wing, Science & Experiment Building, 1
            Weigang, Xuanwu District, Nanjing, Jiangsu 210095, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.7
            Genome Coverage                   :: 30-80x
            Sequencing Technology :: Illumina Miseq; Roche 454 GS Junior
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001900495.1-RS_2024_06_13
            Annotation Date                   :: 06/13/2024 02:21:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,665
            CDSs (total)                      :: 4,551
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 47 of 109
            Pseudo Genes (incomplete)         :: 54 of 109
            Pseudo Genes (internal stop)      :: 31 of 109
            Pseudo Genes (multiple problems)  :: 22 of 109
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP010118.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="C2"
                     /host="cow"
                     /db_xref="taxon:562"
                     /plasmid="A"
                     /collection_date="2014"
     ARG             3..370
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="100.0"
                     /coverage="45.02"
     ARG             7957..8399
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="100.0"
                     /coverage="54.61"
     gene            431..1234
                     /gene="aph(3'')-Ib"
                     /locus_tag="RG25_RS24845"
                     /old_locus_tag="RG25_23710"
     ARG             432..1231
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1234..2070
                     /locus_tag="RG25_RS24850"
                     /old_locus_tag="RG25_23715"
     CDS             1234..2070
                     /locus_tag="RG25_RS24850"
                     /old_locus_tag="RG25_23715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            2376..2618
                     /locus_tag="RG25_RS24865"
                     /old_locus_tag="RG25_23725"
     CDS             2376..2618
                     /locus_tag="RG25_RS24865"
                     /old_locus_tag="RG25_23725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(2650..3300)
                     /gene="tetR(A)"
                     /locus_tag="RG25_RS24870"
                     /old_locus_tag="RG25_23730"
     CDS             complement(2650..3300)
                     /gene="tetR(A)"
                     /locus_tag="RG25_RS24870"
                     /old_locus_tag="RG25_23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            3406..4605
                     /gene="tet(A)"
                     /locus_tag="RG25_RS24875"
                     /old_locus_tag="RG25_23735"
     ARG             3407..4602
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<4572..4760)
                     /locus_tag="RG25_RS24880"
                     /pseudo=""
     CDS             complement(<4572..4760)
                     /locus_tag="RG25_RS24880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(4804..5193)
                     /locus_tag="RG25_RS24885"
     CDS             complement(4804..5193)
                     /locus_tag="RG25_RS24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            5411..5851
                     /locus_tag="RG25_RS24890"
                     /old_locus_tag="RG25_23740"
     CDS             5411..5851
                     /locus_tag="RG25_RS24890"
                     /old_locus_tag="RG25_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(6618..6767)
                     /locus_tag="RG25_RS25960"
     CDS             complement(6618..6767)
                     /locus_tag="RG25_RS25960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            7338..7496
                     /locus_tag="RG25_RS26450"
     CDS             7338..7496
                     /locus_tag="RG25_RS26450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     ARG             1235..2067
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(6803..6976)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          6900..7505
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cattgcggcg ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa
       61 ccgccttgtc cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct
      121 ctcggtgctg gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc
      181 tgtttcgcgc aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc
      241 cgcgacactc gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca
      301 cgagccgcgc cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag
      361 aattcgttaa ctgcacattc gggatatttc tctatattcg cgcttcatca gaaaactgaa
      421 ggaacctcca ttgaatcgaa ctaatatttt ttttggtgaa tcgcattctg actggttgcc
      481 tgtcagaggc ggagaatctg gtgattttgt ttttcgacgt ggtgacgggc atgccttcgc
      541 gaaaatcgca cctgcttccc gccgcggtga gctcgctgga gagcgtgacc gcctcatttg
      601 gctcaaaggt cgaggtgtgg cttgccccga ggtcatcaac tggcaggagg aacaggaggg
      661 tgcatgcttg gtgataacgg caattccggg agtaccggcg gctgatctgt ctggagcgga
      721 tttgctcaaa gcgtggccgt caatggggca gcaacttggc gctgttcaca gcctatcggt
      781 tgatcaatgt ccgtttgagc gcaggctgtc gcgaatgttc ggacgcgccg ttgatgtggt
      841 gtcccgcaat gccgtcaatc ccgacttctt accggacgag gacaagagta cgccgcagct
      901 cgatcttttg gctcgtgtcg aacgagagct accggtgcgg ctcgaccaag agcgcaccga
      961 tatggttgtt tgccatggtg atccctgcat gccgaacttc atggtggacc ctaaaactct
     1021 tcaatgcacg ggtctgatcg accttgggcg gctcggaaca gcagatcgct atgccgattt
     1081 ggcactcatg attgctaacg ccgaagagaa ctgggcagcg ccagatgaag cagagcgcgc
     1141 cttcgctgtc ctattcaatg tattggggat cgaagccccc gaccgcgaac gccttgcctt
     1201 ctatctgcga ttggaccctc tgacttgggg ttgatgttca tgccgcctgt ttttcctgct
     1261 cattggcacg tttcgcaacc tgttctcatt gcggacacct tttccagcct cgtttggaaa
     1321 gtttcattgc cagacgggac tcctgcaatc gtcaagggat tgaaacctat agaagacatt
     1381 gctgatgaac tgcgcggggc cgactatctg gtatggcgca atgggagggg agcagtccgg
     1441 ttgctcggtc gtgagaacaa tctgatgttg ctcgaatatg ccggggagcg aatgctctct
     1501 cacatcgttg ccgagcacgg cgactaccag gcgaccgaaa ttgcagcgga actaatggcg
     1561 aagctgtatg ccgcatctga ggaacccctg ccttctgccc ttctcccgat ccgggatcgc
     1621 tttgcagctt tgtttcagcg ggcgcgcgat gatcaaaacg caggttgtca aactgactac
     1681 gtccacgcgg cgattatagc cgatcaaatg atgagcaatg cctcggaact gcgtgggcta
     1741 catggcgatc tgcatcatga aaacatcatg ttctccagtc gcggctggct ggtgatagat
     1801 cccgtcggtc tggtcggtga agtgggcttt ggcgccgcca atatgttcta cgatccggct
     1861 gacagagacg acctttgtct cgatcctaga cgcattgcac agatggcgga cgcattctct
     1921 cgtgcgctgg acgtcgatcc gcgtcgcctg ctcgaccagg cgtacgctta tgggtgcctt
     1981 tccgcagctt ggaacgcgga tggagaagag gagcaacgcg atctagctat cgcggccgcg
     2041 atcaagcagg tgcgacagac gtcatactag atatcaagcg acttctccta tcccctggga
     2101 acacatcaat ctcaccggag aatatcgctg gccaaagcct tagcgtagga ttccgcccct
     2161 tcccgcaaac gacccctgga agccccgtag cgacgcggag aggggcgaga caagccaagg
     2221 gcgcaggctc gatgcgcagc acgacatagc cggttctcgc aaggacgaga atttccctgc
     2281 ggtgcccctc aagtgtcaat gaaagtttcc aacgcgagcc attcgcgaga gccttgagtc
     2341 cacgctagat ctatctcatc tgcgcaaggc agaacgtgaa gacggccgcc ctggacctcg
     2401 cccgcgagcg ccaggcgcac gaggccggcg cgcggacccg cgccacggcc cacgagcgga
     2461 cgccgcagca ggagcgccag aaggccgcca gagaggccga gcgcggccgt gaggcttgga
     2521 cgctagggca gggcatgaaa aagcccgtag cgggctgcta cgggcgtctg acgcggtgga
     2581 aagggggagg ggatgttgtc tacatggctc tgctgtagtg agtgggttgc gctccggcag
     2641 cggtcctgat caatcgtcac cctttctcgg tccttcaacg ttcctgacaa cgagcctcct
     2701 tttcgccaat ccatcgacaa tcaccgcgag tccctgctcg aacgctgcgt ccggaccggc
     2761 ttcgtcgaag gcgtctatcg cggcccgcaa cagcggcgag agcggagcct gttcaacggt
     2821 gccgccgcgc tcgccggcat cgctgtcgcc ggcctgctcc tcaagcacgg ccccaacagt
     2881 gaagtagctg attgtcatca gcgcattgac ggcgtccccg gccgaaaaac ccgcctcgca
     2941 gaggaagcga agctgcgcgt cggccgtttc catctgcggt gcgcccggtc gcgtgccggc
     3001 atggatgcgc gcgccatcgc ggtaggcgag cagcgcctgc ctgaagctgc gggcattccc
     3061 gatcagaaat gagcgccagt cgtcgtcggc tctcggcacc gaatgcgtat gattctccgc
     3121 cagcatggct tcggccagtg cgtcgagcag cgcccgcttg ttcctgaagt gccagtaaag
     3181 cgccggctgc tgaaccccca accgttccgc cagtttgcgt gtcgtcagac cgtctacgcc
     3241 gacctcgttc aacaggtcca gagcggcacg gatcactgta ttcggctgca actttgtcat
     3301 gcttgacact ttatcactga taaacataat atgtccacca acttatcagt gataaagaat
     3361 ccgcgcgttc aatcggacca gcggaggctg gtccggaggc cagacgtgaa acccaacaga
     3421 cccctgatcg taattctgag cactgtcgcg ctcgacgctg tcggcatcgg cctgattatg
     3481 ccggtgctgc cgggcctcct gcgcgatctg gttcactcga acgacgtcac cgcccactat
     3541 ggcattctgc tggcgctgta tgcgttgatg caatttgcct gcgcacctgt gctgggcgcg
     3601 ctgtcggatc gtttcgggcg gcggccggtc ttgctcgtct cgctggccgg cgctgctgtc
     3661 gactacgcca tcatggcgac ggcgcctttc ctttgggttc tctatatcgg gcggatcgtg
     3721 gccggcatca ccggggcgac tggggcggta gccggcgctt atattgccga tatcactgat
     3781 ggcgatgagc gcgcgcggca cttcggcttc atgagcgcct gtttcgggtt cgggatggtc
     3841 gcgggacctg tgctcggtgg gctgatgggc ggtttctccc cccacgctcc gttcttcgcc
     3901 gcggcagcct tgaacggcct caatttcctg acgggctgtt tccttttgcc ggagtcgcac
     3961 aaaggcgaac gccggccgtt acgccgggag gctctcaacc cgctcgcttc gttccggtgg
     4021 gcccggggca tgaccgtcgt cgccgccctg atggcggtct tcttcatcat gcaacttgtc
     4081 ggacaggtgc cggccgcgct ttgggtcatt ttcggcgagg atcgctttca ctgggacgcg
     4141 accacgatcg gcatttcgct tgccgcattt ggcattctgc attcactcgc ccaggcaatg
     4201 atcaccggcc ctgtagccgc ccggctcggc gaaaggcggg cactcatgct cggaatgatt
     4261 gccgacggca caggctacat cctgcttgcc ttcgcgacac ggggatggat ggcgttcccg
     4321 atcatggtcc tgcttgcttc gggtggcatc ggaatgccgg cgctgcaagc aatgttgtcc
     4381 aggcaggtgg atgaggaacg tcaggggcag ctgcaaggct cactggcggc gctcaccagc
     4441 ctgacctcga tcgtcggacc cctcctcttc acggcgatct atgcggcttc tataacaacg
     4501 tggaacgggt gggcatggat tgcaggcgct gccctctact tgctctgcct gccggcgctg
     4561 cgtcgcgggc tttggagaaa ttcttcaaat tcccgttgca catagcccgg caattccttt
     4621 ccctgctctg ccataagcgc agcgaatgcc gggtaatact cgtcaacgat ctgatagaga
     4681 agggtttgct cgggtcggtg gctctggtaa cgaccagtat cccgatcccg gctggccgtc
     4741 ctggccgcca catgaggcat gttccgcgtc cttgcaatac tgtgtttaca tacagtctat
     4801 cgcttagcgg aaagttcttt taccctcagc cgaaatgcct gccgttgcta gacattgcca
     4861 gccagtgccc gtcactccca ctcttacatt gttcgcagta tctaggtaca tgttattgcg
     4921 tgcaccttca tgatgtataa tgttattaat gacagcagaa agatcgacag aattaatatt
     4981 cttcgtacct gggacagtta tcgggtgtcg gccagtgatg agaccaaagg atgcagtata
     5041 agatgaacca ctactattat catggccacc attaccacca ccgttaaaac tacctcccga
     5101 acttccagag ccgctatgac catcattgta ggggtcattt ggtcccatgt gaccaaaacc
     5161 accgacagta ccgctattta actctgtact cataaaatta tcctctttga cttttaaaaa
     5221 cagcaaaaac tgtatttata acaatgtgtt tatatacagt attcgaaata ctacgcccgt
     5281 caaaaccaca ccgtcaacag gttttttgtg tatttttcac gcctcacggg cagcctcaga
     5341 ccttttgtgt taaaacagta atatgttctg ctgttacaat acggtacaca caggagacac
     5401 tgtcactttt atggcatggc acacagttca agagacactg aaattaacag gaaagtcgcg
     5461 ctcgcaatta tatcgtgaca tggctaaagg ccttgtcagc tatcgcacag gaaaaaatga
     5521 tcgccgggag ttcgagacca gtgaactgat tcgggcctat ggcgagctgc gcacttctga
     5581 gacaccaaaa aggcacaagg agggacactc caaatctgtc gttagccagg cggaaaatgc
     5641 tcagctagac gtcattcagc agaaactgga cgctctgcag cagactgtta ccctaatgct
     5701 tgaagacaag atggcgcggg aagcagaaaa ccgcctgcga aatgaggaga gggaacaact
     5761 acaggccgaa attagccgtc tggctgacgc gctggcacag gaaaaaaagc ggggattctg
     5821 gtcgcggctg tttcgctccg gttcagagta acagtgcagg tgactctgat ttaccataac
     5881 atctgttatc agcaccgcct gagaggccct cagggcgttt ctgacgggaa accggtaaaa
     5941 caggcaacaa accacccgaa aacccgccag aaacgcgcag gctctgtttt tacggcatgc
     6001 atgagtatgc gcacaagtgc atagcgcgga aattcacacc attcagccgg ggtaagtcct
     6061 gtttccggcg ttcttcccat ccgaaaacgg tgaacgggga cgcaatttat tgcgtggccg
     6121 tgaagcgttg ccggcgcagc cggaaaaccc gctgtcagca cgttcaggaa gggggtgatg
     6181 tacaaatggc acagaggatg aaagtgacgt atgagctgaa ggaatgatgt aaaagtgatg
     6241 tataagtggt gtaaaagtga tgtataagtg atgtaaaagt gatgtatgaa ctgaaggagc
     6301 gatgtaattc tgtagtcagg aagaataacc cgttcatgca ggcatgaacg atacagtgag
     6361 gaacgctttt tttgtcacga tctcccctta acctgcgtaa atcacccgcc atggttgatt
     6421 atttttcaac ctcaaaatgg tcatacacct aacgcagccg cgattttcat cgcaactgca
     6481 tgaattaaaa gaaaaaaaag gaatatttcg ttttcacgag taatacccgg gtattaaaaa
     6541 tccactgtaa ttacatgaaa ataaagaaat aaaaggtaat accggagtat taccacgtac
     6601 gcgggcaagc gaaaaagcta taacgcttcc cggccgacag gcgacccaaa atccacaggg
     6661 gcatgcaggt tctcttcagt aacctgcgcc attaaatcaa aaaggtcata ttcgccggga
     6721 agctgcgtat cagtcgaatc cagttgttca tcatattcag atggcatggg gtgatatcca
     6781 gtagctgaga ggttgttagc gggtttcggg gcgcagccct gaaccagtca tgtagcgcta
     6841 gcggagtgta tactggctta ctatgttgcc acgattgcga aaatcagtga agtgcttcat
     6901 gtggcaggag aaaaaaggca gcaccggcgc gtcagcagaa tatgcgatac aggagatatt
     6961 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtaccgg
     7021 cttacggacg gggacggaaa tttcctggaa gataccagga agacacttag caaagaagag
     7081 ataaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag catcgcaaaa
     7141 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc
     7201 cccctggtag ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
     7261 cgctgttatg gccgcggttt ctcattccac gcctgacact cagttccgtg aaggcagttc
     7321 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg
     7381 gtaactatca acttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca
     7441 ttagtaacag gagtgcacga gaagatagat taagaggtct tgaagtagag gcctgactgc
     7501 ggctacactg aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt accgggttaa
     7561 gcagttcccc aactgactta accttcgaac aaaccgcctc cccaggcggt tttttcgttt
     7621 acagagcaag agattacgat tcattgaaaa ggatctcaag aagatccttt gtagattact
     7681 atcaattaat cttccgaaca tgacaccagt cactgttgat tacggttaaa ccgctccgta
     7741 acaggtttcc ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga taaatatatc
     7801 tacttattga gagatgatat atttatttcc aaaatgctac agatacatca tcatgaaacg
     7861 gatcaccgca aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgag
     7921 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
     7981 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
     8041 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
     8101 cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg
     8161 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
     8221 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
     8281 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
     8341 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
     8401 a
//
