LOCUS       NZ_CP008850             8737 bp    DNA     circular CON 21-APR-2024
DEFINITION  Acinetobacter baumannii strain AC29 plasmid pAC29a, complete
            sequence.
ACCESSION   NZ_CP008850
VERSION     NZ_CP008850.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02714232
            Assembly: GCF_000695855.3
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Lean,S.S., Yeo,C.C., Suhaili,Z. and Thong,K.L.
  TITLE     Comparative Genomics of Two ST 195 Carbapenem-Resistant
            Acinetobacter baumannii with Different Susceptibility to Polymyxin
            Revealed Underlying Resistance Mechanism
  JOURNAL   Front Microbiol 6, 1445 (2016)
   PUBMED   26779129
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8737)
  AUTHORS   Lean,S.S., Suhaili,Z., Yeo,C.C. and Thong,K.-L.
  TITLE     Comparative genomics of polymyxin-resistant and susceptible
            Acinetobacter baumannii isolated from the same patient
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8737)
  AUTHORS   Lean,S.S., Suhaili,Z., Yeo,C.C. and Thong,K.-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUN-2014) Institute of Biological Sciences, Faulty of
            Science, University of Malaya, LabA407, Laboratory of Biomedical
            Sciences and Molecular Microbiology, Kuala Lumpur, Wilayah
            Persekutuan 50603, Malaysia
COMMENT     ##Assembly-Data-START##
            Assembly Method :: CLCBio Genomics Workbench v. 5.0
            Sequencing Technology             :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000695855.3-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 01:52:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,807
            CDSs (total)                      :: 3,737
            Genes (coding)                    :: 3,637
            CDSs (with protein)               :: 3,637
            Genes (RNA)                       :: 70
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 19 of 100
            Pseudo Genes (incomplete)         :: 73 of 100
            Pseudo Genes (internal stop)      :: 15 of 100
            Pseudo Genes (multiple problems)  :: 7 of 100
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP008850.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8737
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="AC29"
                     /isolation_source="Endotracheal Secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pAC29a"
                     /geo_loc_name="Malaysia: Kuala Terengganu, Terengganu"
                     /lat_lon="5.311737 N 103.119912 E"
                     /collection_date="2011-07-31"
                     /collected_by="Lean Soo Sum"
     gene            join(8688..8737,1..532)
                     /locus_tag="BL01_RS18535"
                     /old_locus_tag="BL01_p0005"
                     /pseudo=""
     CDS             join(8688..8737,1..532)
                     /locus_tag="BL01_RS18535"
                     /old_locus_tag="BL01_p0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /name="plasmid replication DNA-binding protein"
     gene            552..695
                     /locus_tag="BL01_RS19550"
     CDS             552..695
                     /locus_tag="BL01_RS19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(884..1402)
                     /locus_tag="BL01_RS18540"
                     /old_locus_tag="BL01_p0010"
     CDS             complement(884..1402)
                     /locus_tag="BL01_RS18540"
                     /old_locus_tag="BL01_p0010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            1775..2062
                     /locus_tag="BL01_RS18545"
                     /old_locus_tag="BL01_p0015"
     CDS             1775..2062
                     /locus_tag="BL01_RS18545"
                     /old_locus_tag="BL01_p0015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            2049..2363
                     /locus_tag="BL01_RS18550"
                     /old_locus_tag="BL01_p0020"
     CDS             2049..2363
                     /locus_tag="BL01_RS18550"
                     /old_locus_tag="BL01_p0020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            2491..4902
                     /gene="znuD2"
                     /locus_tag="BL01_RS18555"
                     /old_locus_tag="BL01_p0025"
     CDS             2491..4902
                     /gene="znuD2"
                     /locus_tag="BL01_RS18555"
                     /old_locus_tag="BL01_p0025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            5207..5668
                     /locus_tag="BL01_RS18560"
                     /old_locus_tag="BL01_p0030"
     CDS             5207..5668
                     /locus_tag="BL01_RS18560"
                     /old_locus_tag="BL01_p0030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(6164..6535)
                     /locus_tag="BL01_RS18565"
                     /old_locus_tag="BL01_p0035"
     CDS             complement(6164..6535)
                     /locus_tag="BL01_RS18565"
                     /old_locus_tag="BL01_p0035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            complement(6807..7061)
                     /locus_tag="BL01_RS19875"
                     /old_locus_tag="BL01_p0040"
     CDS             complement(6807..7061)
                     /locus_tag="BL01_RS19875"
                     /old_locus_tag="BL01_p0040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     gene            7745..8695
                     /gene="repM"
                     /locus_tag="BL01_RS18580"
                     /old_locus_tag="BL01_p0045"
     CDS             7745..8695
                     /gene="repM"
                     /locus_tag="BL01_RS18580"
                     /old_locus_tag="BL01_p0045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     Typing          2..532
                     /name="rep_cluster_1903"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7746..8695
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggatagatca accatttaca gagctgtacg taatggacgt ttatcacgct ccagtgatgg
       61 acaattcgat ctagcagagg tcatacgatg ctttggagaa cctgagcaaa catctcaaaa
      121 aattgaatca tctaagcaag aaggtgatga atctacaaaa aaacttattg cccatttaga
      181 aaatgaagtc aaaaaatacc aagaacgtga agaacggtta atgcaacaaa ttgaccgtat
      241 gcaaacactc attgagctga aaagtgttgc acctgccaca gcagcaccac accaagatgc
      301 tacggcatgc gacaccaaga tgccacagca tgcgacaaca caacaagaca ctgataacaa
      361 aaagaataat gaattaaata ttgcagaaaa tgtagcagtg ccacagcaag aaactacggc
      421 ataccacacc caaacgctac agcatgccac gttgcaaagt gtggcagtgc cacaacacaa
      481 aaaacgtggt ttatttggcc gtgtgctgaa tgccgtcttt gataatgact gagcaataag
      541 ggtgaaagat catgccgaaa ctgaaagaca ttgccctggg aattattgta gctccgctgc
      601 tgatcccgat catgctgatt gcatcgtacc aggataaaaa ggcgctgaag aaagagctgg
      661 atgaatgcca aaaagaaaaa gaccaggcat cctgattatt caattgagct aatcaaaagt
      721 gtggaacatt gatgaaataa tagcgtggaa cgtgaaaagt cataaaaaaa gcccgaacag
      781 ggatgctcgg gctataaact agaaactaca gcgttgaatt ataaggataa ttatacagca
      841 tttcgtataa ggtgtattat gttaatttta ggaaatatta aaactaattt tcagacttat
      901 cccaccaact ttgagctaaa tcagtatttt taggaatacc tataccatca cgatacataa
      961 cggaaagact tcgcattgct ttcttattac ctaactttgc agccttttca cgaagttcaa
     1021 aagctttatt aatattaact tctattccgc tatctgcacg agtatataga tcagctaact
     1081 caaccattga ttcagaatgg ccattttctg aggctttctg aaaccactta aaagcttttt
     1141 ggaaatgttc taaagaatcg tttttgtcat catcttccat taatctagca taagtaacat
     1201 atccatcagc taaccaaaac attgctgcta catttccatt ttctgctgat ttaagagccc
     1261 aaacttcagc ttgattttca tctgaatcaa caccatgacc atatagataa atctcggcta
     1321 attctaactg agcagaagta tctccatttt ctgctttcgc taataaatca ttagaaaaaa
     1381 caggttctgc aaatgtttcc attgaaaggc aaactcctaa agccataaaa atatttgtta
     1441 atttattcaa tttttaacct ttaaactaaa ctgttaggct tgtatatttt tatatcatac
     1501 ctaaaagaaa actctaaaat tagagtatgt cttatctcta tttaacataa tggcgattat
     1561 acgaagtaca cttgtatatt aatataaata tcaactactt aataaaaata gaaaaatccc
     1621 aaaatgccca aaaagccgac tttgaaacaa gtcggctttt ttatgagcag ttttgatact
     1681 tcttgccaat aattttgact aaaaattaca cgaatctggt tgagtgatac aattttgtga
     1741 tacattattg tatatacaaa tttgaatgtt ttttatggaa cagtattttg aatgggatga
     1801 ggctaagaat cgaaagaatc agaaaaagca tgatatctct ttcgaaactg caagccttgt
     1861 ttttgaagat ccattacgga tctcaatcca agacagacat accaatggtg aagaacgttg
     1921 gcaaaccatt ggacgagtta aaggcgtact aatgttattg gtggctcaca ccatttttga
     1981 tgaagatgac tgtgaaatca tccgaatcat tagtgcaagg caagtcacta aagcggagcg
     2041 aaacaaatat gagcatggtt agatactcac acaaagagct gaatgagaaa tttggtgaga
     2101 agcaagatgc tgaaattcaa cgcttgcttg ctaaaggcac ggttcctgat gatcagctag
     2161 atctatcaga tattcctgaa attacagact ggagcaatgc tgtacgccaa aatcaatttt
     2221 accgcccagt aaagcaacaa acttcaattc ggttagatgc tgatgtactt gcttggttca
     2281 aagcacaagg caaaggttat caaacacgta tgaatgaaat tttacgagat gctatgctaa
     2341 aagaattaaa aaatcatcaa taagaatggg agcttaggct cccatttttg acttcatttt
     2401 taggggggat taccattcaa aaatttaaat agtagatttt ataaattttt aagtgttata
     2461 ttataatata acattaaatt tggttgtttt atgctaaata aaagtaaact atttttagct
     2521 ttaattactt taggggcctc gaaaatttta ttggcagctg aagggcctgt tactacctta
     2581 aatacaatag tattaactgc acaatctgat gagttagggt ctgagctgtt aggcaaaagt
     2641 ctaaatgtta gtaatcagtt tattgatact agtaaactta aacagcgttc gacaactcta
     2701 ggggatgcct tgggtacgga attaggaatt cattctaatc aatatggagg tggagcatct
     2761 actcctatta ttcgtgggca ggaaggaaaa cgtattaaag ttttacagaa taatgctgac
     2821 gtgttggata tgtctaatat gtccccagat cacgctgtca cagttgaacc aagtttagct
     2881 aagtctattg aaattatacg tggtgcaagt acccttttgt atagttcaaa tagtgcggca
     2941 ggggtagtca atgtcattga ctataaaatt ccaacccaaa tgcctcaaga cggattagag
     3001 ggtaatacta cattacgttt taatactggc agtaatgaga agttgaccac agcaggtgta
     3061 acagttggct taagcccaca tgtagcttta cgggcagaag ggttatatcg taatgctggt
     3121 aactataaaa ctcctcatta ccagtctagc tcttataatt ctttagaaga tcttgaaaac
     3181 caaaacagta tttataaaaa tttaaaatat ttaccagaaa gctgggcaga gtcgagagta
     3241 ggtacgcttg gattatcctg gatagacgat aatacttatt taggagtttc ttatacacat
     3301 cgtcatgatg aatatggttt accagcccat agccatttat atgaaggttg tggtgcctct
     3361 gcaatcggta tcgattcacg aattagtgga ttaaaaaatt atcttttata ttatcctcaa
     3421 ttgatggatg agcaagatat taattacata aatccacgtc ctgattgtca tcagcataat
     3481 catattcatg aaactaattt ctctcataat gctccttata ttgatttaaa tactcgacgt
     3541 tatgatgtgc gtggagaatt cactcaacca tttaccggta ttgacaaaat tcgtacctca
     3601 ttgagttata tagattattt tcacaatgaa ctcgagggag ataagattac taactttttt
     3661 aaaaatacag gaaaagtagg ccgcatagag ttatcacatc agccattagg ggaattaacc
     3721 ggtatcttgg ggctacaata tttggaacag gataatagtg ctttatcacc agtccattct
     3781 caagaaggcc ataccaccta tctagataat cagcagctat taaatcgaaa tgtcactaaa
     3841 aactttagtg tatttggcct agaaaaatat aactggaacg actttacttt cgaactaggt
     3901 gctcgtatag aaaagcagaa agttagtatg gattatgata tagaaaaaat taaagattca
     3961 atgaaacctt ggcctaataa atataacagt ccttatgtag aaaaaaataa taaaatcagg
     4021 gctcaaaatt taaaatctat tttagaagct gttcaaccta acaaagaaac agctttttct
     4081 tatgcaggta cagtccactg gagatttgct ccaaattata tcttatcatt aacaggtaca
     4141 catcaagaaa gattacctaa tgcacaagaa atgtatactc atggtatgca tctcgctact
     4201 aattcttttg aaataggtaa ccgctttcta agaaaagaga aatccaataa tttagaaatc
     4261 agtctggcct ataaggatga tttacttgat tatcaaatca gtacttacta ttatgatttt
     4321 gataactata tatatttaca gacactcaat gaagttttag gaactacaaa agttagagat
     4381 cagcatacac tacgtattaa tcattatagc cagtctgctg ctaattttta tggattagag
     4441 ggcaacatag ggtatcaatt taattctgtt tatcacggct ctctttttgg agattatgtg
     4501 aaaggccgtt taacaaattt acctgatgct gtaattgcat acgatatctg gaatagagaa
     4561 ccgactttag ctccccaaaa ggatcgttac actcctagat tacctcctgc tcgtttgggt
     4621 acacgactaa aagctgattt tgatgaatcg cttaaaggtg aaatagaata ttatcgagtc
     4681 tttaaacaag ataatatttc taaatttgag caggttacat caggttataa tatgcttaat
     4741 atgactttag catataaaaa taagctatct cacacagaat atgatttatt ctttaaagcg
     4801 aataatttac ttgatcagaa agtctatgcc catgaaacat ttttgccata tattccccaa
     4861 attggaagaa attttagttt aggcttaaat cttaatttct agataagata ctgtaatctt
     4921 ctaagaaaat ttcttagaag atttttctta taaaatgatt agattaataa ttaaggtaag
     4981 cgtccgctga atcccatacc tattttttaa ttcgatttag gtttccagca gtgtggcagt
     5041 aactcttcaa tctgggtcac tttatgtgtc ggcagccttt tcagcactga atagccaccg
     5101 attttgtaga caccttttag ctagataaaa agcaattttt ttgatcttta aagaatgagg
     5161 atataagctg tttaaaactt aattctaatt ggttaatagg tgaaaaatga aattagcaga
     5221 agctctctta cttcgaagtg atcaacaaaa aaaaattatt tcactaaaac aacgtatcaa
     5281 tgcaaatgta ttggtacaag atggtgatca accatctgaa gatccaaatg aactactaaa
     5341 acaagtattt tctttaattc aagaatttca aaaattaagt tatgcaattc atgaaactaa
     5401 tgctttaaca aaattaaatg atggccgttc tctacttgct ttattgactt tgcgtgatga
     5461 atttgtagaa cagcacaaaa ccctaactgc agcaataagt aatacatctc gtgaatctga
     5521 tcgttatagt acgcgggaaa taaagtggca taaagttatt ccagtttcat cattacaaaa
     5581 acaagctgat gatattagtc ttaaattacg tgatttaaac gtgttaattc agtcgactaa
     5641 ttggaaaatt gatttgattg aagcctaata gtcctttaga tataatcaag ttgaacatac
     5701 tgggcgagta caagatccct tacttccaca acataggatt gtaaataaag tgtagtggct
     5761 tgcaaagcaa gtggataagc ttaaaatttt aagtacaaat caatccatca cttggcataa
     5821 ctcaaatctt tgtgcaaaca aagttacacg ttacttataa ctaccatgta ctgacagtat
     5881 gttcacctca attactaaaa ataaagaaat gaacaagcat cagctcaact acatttctat
     5941 ttttaattca gttcggcatt taacataaaa tctcttatac gaagcccagg ttaattctgg
     6001 gctttttgtt caatctcatc attggccgag aacatacttg gattcgtaag cctgatattt
     6061 tcctgacaac ttccacacag catcaatcct gcaaaaagtt gtacattttg tctattttca
     6121 caaagcgtac atggctttga aagctgaata atattatctg acactacttt accctcttat
     6181 agttatcatc tgcatatgaa taacctttag cttgtatcat tctaacctca tctttagaag
     6241 cgccattttt cattaagagc cacaacatca tgaaatatag tccagaaaag tcttcatagt
     6301 atttttcaga aaaattgtta tcttcctcaa aatcatctat tcgcttttct aatcgattca
     6361 ccagatcctc aatacgatcg gcttgctgtt taacagcaat agattgaaaa tcattggtca
     6421 aaattttact gaataattta ctggttctga ttccttctga attagccaat tgatcgtaga
     6481 attctgctac ttgtggatct aacctgactg cataagtttt gactttttcg cccataaatt
     6541 acacctctta ttaaaattag attttgtaca aaattaaata tatataattc aactaattaa
     6601 ataaaaaata ataattagat tacgtacaaa cttatttgta cagaattaat ttattctaaa
     6661 acaaaaactt atttaaatat aatgcttttt ttatgtacaa tcatgagtac atattttttc
     6721 ttaaatttat caatatctta tctaaaaaaa gtgttgtttt gatgtacaac catgagcaca
     6781 gcataaaaaa tagataattc aatcacttat tctaaaaaaa gaccattttt ctgtacacgt
     6841 ttctgagacc ctaaaaaatc gctgaaatcc ttatcctgta aggctttgag gggtgtcatc
     6901 gttagatgcg tggtgtgtga caaaaaaatc ccggcatgtg ccggatcttg gattggaaaa
     6961 ttggctaaag tgacgtaggg ccggtgcttg gttttgcatg gaaaaaagta tttatttcct
     7021 tgtttagaaa aacgtaaaaa atcaattttt tgtccattca tccaggttaa aaatttcaac
     7081 ccaaaacttt aattatgaaa agcttcatag aacgcgttca aatgcgattt aagagccttt
     7141 acctagaaaa catagatctc atagtgaaaa acacaaaaca gctcaaaaaa cgcaaatgag
     7201 agcgaaataa agaggtattt tgactttaga tagatgcata gagcgagtgt ctacgagcga
     7261 actatcaaaa tttgcgccta gaccctctga aaaacatttt tttgtcctct ttagcctaag
     7321 aaagcgtaag tttcgtgcag aaatttgctc ctggatcgag cgtagcgagt aaaaaagttt
     7381 tatgagcgaa gcgaattcca agtttctttt gctttttttt aaagtcacgc cagtattaac
     7441 caaaaaattg ccccaacgaa ctgagcgaaa gcgaagttca atagagtttg agcgaagcga
     7501 aaaccaaggg caatttttca ttccctgggc ttttaattat tttaaagttt ttaaatactt
     7561 ttaaatggct tgaaagcctt atgaataaag ggttttagct cttatatgtc cacgtttacc
     7621 ttgcaatatg tccacgttta ccttgcaata tgtccacgtt taccttgcaa tatgtccacg
     7681 tttaccttgc attagtacac aaataatatt aacgtgtact tatacacaat aaaaaatagt
     7741 ggctatgaga gatttagttg taaaggacaa tgccttaatc aacgcaagct ataacttaga
     7801 cttagtagaa caacgtttaa ttttattggc tattgttgaa gcaagggaaa gtgggaaagg
     7861 gattaatgca aatgatccat tagaagttca tgcagatagt tatatcaatc aatttggtgt
     7921 acaccgtaat acagcttatc aagccttaaa agatgcttgt aaggatttat tcgcgcgtca
     7981 atttagctat caagagaaaa aagctaatgg gaatatccga aatgttatga gtcgttgggt
     8041 atctcaaatt gcttataacg acaatgaagc aactgtagac ttaatatttg cacctgctgt
     8101 tgttcccttc ataacccgac tggaagaaca atttactaaa tatgaattac agcaagttag
     8161 tagtcttagt agcgcttatg ccattcgctt atatgagctt ctaattcagt ggcgaagcac
     8221 tggtaaaacc ccaaccatag aactacaaga atttagaaag aagttaggcg ttcttgataa
     8281 tgaatattta cggatggctc atttaaaaga gcgtgtttta gagctttcaa ttaaacaaat
     8341 aaatgagcat acggatataa ctgtaaaata tgaacagcat aaaagaggac gttctatttc
     8401 aggattttct tttaccttta aacagaagaa gaaggatagc ccatcaatag aaagagatcc
     8461 gaacactttg gagctttttt caaagatgac cgatgctcaa cggcatatgt ttgcaaataa
     8521 actttcagaa ctccctgaaa tgggtcgcta ttcacaagga acagaaagct accaacagtt
     8581 tgctgtacgt attgctgaga tgctacaaga tcccgctcaa ttcaaagagc tatacccata
     8641 cctaaaaaaa gtgggataca tgccatcaaa taaaaaggac accgtaaatg gctaagttat
     8701 cactaagtga agtatctaaa aaatttcatg tggatag
//
