LOCUS       NZ_CP008845             3514 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Klebsiella michiganensis strain M1 plasmid pKOXM1D, complete
            sequence.
ACCESSION   NZ_CP008845
VERSION     NZ_CP008845.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02887315
            Assembly: GCF_000724525.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella michiganensis
  ORGANISM  Klebsiella michiganensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Shin,S.H., Um,Y., Cho,S., Kim,J., Roh,H. and Yang,K.-S.
  TITLE     Whole Genome Sequence of Klebsiella oxytoca M1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Shin,S.H., Um,Y., Cho,S., Kim,J., Roh,H. and Yang,K.-S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2014) Life Science Institute, Macrogen Inc., 254,
            Beotkkot-Ro, Seoul 153-781, Republic of Korea
COMMENT     ##Taxonomic-Update-Statistics-START##
            This Genome (query)               :: GCA_000724525.1
            Current Name                      :: Klebsiella michiganensis
            Previous Name                     :: Klebsiella oxytoca
            Date Updated                      :: 2016-07-08
            Analysis Type :: Average Nucleotide Identity (ANI)
            Analysis 1 (A1) :: Query vs. PROXYTYPE genome for current name
            A1 Genome (subject)               :: GCA_000633235.1
            A1 Name                           :: Klebsiella michiganensis
            A1 ANI                            :: 98.88235%
            A1 Query Coverage                 :: 84%
            A1 Subject Coverage               :: 91%
            Analysis 2 (A2) :: Query vs. PROXYTYPE genome for previous name
            A2 Genome (subject)               :: GCA_000507385.1
            A2 Name                           :: Klebsiella oxytoca
            A2 ANI                            :: 92.55049%
            A2 Query Coverage                 :: 78%
            A2 Subject Coverage               :: 84%
            ##Taxonomic-Update-Statistics-END##
            ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-JUN-2014
            Assembly Method :: Newbler v. 2.8; SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 327.0x
            Sequencing Technology             :: 454; Illumina HiSeq; ABI3730
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 01:42:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,991
            CDSs (total)                      :: 5,876
            Genes (coding)                    :: 5,686
            CDSs (with protein)               :: 5,686
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 110 of 190
            Pseudo Genes (incomplete)         :: 99 of 190
            Pseudo Genes (internal stop)      :: 26 of 190
            Pseudo Genes (multiple problems)  :: 36 of 190
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP008845.1.
            The organism was changed from 'Klebsiella oxytoca' to 'Klebsiella
            michiganensis' in July 2016.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Klebsiella michiganensis"
                     /mol_type="genomic DNA"
                     /strain="M1"
                     /isolation_source="soil"
                     /db_xref="taxon:1134687"
                     /plasmid="pKOXM1D"
                     /geo_loc_name="South Korea: Maripo beach"
                     /lat_lon="36.78 N 126.14 E"
                     /collection_date="2012-08-15"
     gene            241..738
                     /locus_tag="HR38_RS31395"
                     /old_locus_tag="HR38_30175"
     CDS             241..738
                     /locus_tag="HR38_RS31395"
                     /old_locus_tag="HR38_30175"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            745..960
                     /locus_tag="HR38_RS31400"
                     /old_locus_tag="HR38_30180"
     CDS             745..960
                     /locus_tag="HR38_RS31400"
                     /old_locus_tag="HR38_30180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1374..1565)
                     /locus_tag="HR38_RS29445"
                     /old_locus_tag="HR38_30185"
     CDS             complement(1374..1565)
                     /locus_tag="HR38_RS29445"
                     /old_locus_tag="HR38_30185"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040118553.1"
                     /translation="MLKKNEIILIKSCGNSKTKIFLRGVDEGLMIDASFEDIKFAINNN
                     LHDLLGIQAETTKLKLAK"
                     /name="hypothetical protein"
     gene            2736..3077
                     /locus_tag="HR38_RS29450"
                     /old_locus_tag="HR38_30190"
     CDS             2736..3077
                     /locus_tag="HR38_RS29450"
                     /old_locus_tag="HR38_30190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          2479..2651
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.655"
                     /coverage="100.0"
                     /evalue="9.54e-56"
     Typing          complement(2008..2554)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.909"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          4..1076
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3068..3513
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="5.71e-93"
ORIGIN
        1 acccggagaa ccggcgggcg ctggtcacgc cttcaggact gccagaaacg aagcaggaag
       61 ccgcagagac gattacacgg ggtttgctga tgttagcctc atcaggagag ctgaaaacgc
      121 gtgatgacgt cacaggggcg ctcacagcgg ccggttttga ggtggtcaga accacaaaaa
      181 acagtatcag cattgccgac ccggacgggg ggcgaaatat ccgactgagg ggagccattt
      241 atgagcagtc ttttaacggc ctcgagggac ttcgaggaga aatcgaaagc acagcagcag
      301 cttaccgaag agaggctgaa agccgcattc agcaagcacg agaacgttgt caaatcggaa
      361 ctgaacgcaa gcgcgaagag aatcaacgac gccatcagcg cccacgaaca ggggatgaac
      421 gcggcgatgc agtcgaaccg gctgagcgtg ctgcgaatgg tcgggcggac gtggctgacc
      481 atcaccatgg tgtcagggct gctgttcgcc agcctgagcg gggtgctgtg gtaccagggg
      541 aaccggatag cggcgaacct ggcggaaatc aggcagcaga gagatacgct gtcgaaactg
      601 cacatgcaga cgtggggcgt gacctatctg gagaaccgca acggacggtt tctggtgctg
      661 ccggagggga tgaaagccga aacgggccgg acggtggaca acaagacgcg gaacgcggtg
      721 aagctggtac gggagtagag cagaatgacg gaactggaaa aacaattgct gagcgcgtta
      781 gagcagctac agcaggacta ctcgagaagg ctggacgagt gggagagcgc cttcgaggaa
      841 tggcggaaga tgtctggtct tatgcaacgg gagaacgcgg cgctgagcga gcgcgtgtcg
      901 ggcttgagca ggcaggtgca gagtttgagc gagcagctgc gccggttatc gacaggctga
      961 atgagattga gcaacagcga tacgggcaaa caacggaaaa ggaaccagag aagacagcag
     1021 atctggtacc ggaacggcaa cgcacattat ctcatggtga cagggagtat ggagactgat
     1081 aaacgcccgt ctcaggtggc ctgagcgtga cggggatcat ggccattttc tgcgatttca
     1141 gcacacagag agtctttttt aaccgaaatc accggctatg cagtgatgtg aataagtgat
     1201 gcatatgcgt aaaataaatc aggtgcgttt ctggtggttt tcggtgtact tgccaccacc
     1261 ttttgcccgt cagccatcag aaacgccctg agtgcctccg gcgcggtgcg ggtaagaaga
     1321 cgttatggta aatcggtggc tttatttcga tccttcatgc ttcaggggct gtatcacttt
     1381 gctagtttta gttttgtggt ttcggcttgg attccgagaa gatcgtggag gttattgttg
     1441 atagcaaatt ttatatcttc aaaacttgca tcaatcatga gtccctcatc cacacctcgt
     1501 aaaaaaattt ttgtttttga gttaccacat gattttatta agataatttc attttttttt
     1561 aacatgacat tgtcattgtt tgttgtcgtc agatgaatca ttatcgttgt ttttgtgtgt
     1621 gtttgttaag gctgctaaga ttattcctaa ccactgattt tgtacatatc atcaaaaaaa
     1681 taaaaaaact aatgccctgt ggtgggtatg taacaaatca cggggagcgg gtcagaacta
     1741 cgccaaaact ttgaatgatc gggtggcagg ctaaatgaaa tcttgagatt cattcagcct
     1801 cgtcgtaatc tcttgctctg taaacgaaaa aaccgccttg cagggcggtt tttcgaaggt
     1861 taagtcagtt ggggaactgc ttaaccaggt aactggcttc agcagagcgc agataccaaa
     1921 tactgtcctt ccagtgtagc cgtagttagg ccaccacttc aagaactctg tagcatcacc
     1981 tacattaact cgctctgcta atcctgttac cagtggctgc tgccagtggc gctttggcgt
     2041 gtctttccgg gttggactca agacgatagt taccggataa ggcgcagcag tcggactgaa
     2101 cggggggttc gtgcatacag tccagcttgg agcgaactgc cttcccggaa ctgagtgtca
     2161 ggcgtggaat gagataaacg cggccataac agcggaatga caccggtaaa ccgaaaggca
     2221 ggaacaggag agcgcacgag ggagctacca gggggaaacg cctgggatct ttatagtcct
     2281 gtcgggtttc gccgccactg atttgagcgt cagattttgt gatgcttgtc aggggggcgg
     2341 agcctatgga aaaacggctt cgccgcggcc tcatcgcttc cctgttaagt ttcttcctgg
     2401 tatcttcctg gaaatttccg ccccgcccgt aagccggcac cgctcgccgc agccgaacga
     2461 ccgagcgcag cgagtcagtg agcgaggaag cggaatatct tctgtatcgc atattctgct
     2521 tacgctctgc tgcagcattt ttcttctgcc gcataaggcg ctttcctgat tatcgcactc
     2581 atgccaacat actaagccag tatacactcc gctagcgcta cgtgactggt tcagggctgc
     2641 gccccgaaac cccactgacg cgcctgcggc ttgtccaacc ccgcgccgac cgggaaaggc
     2701 tttcccgtcc gtcacggcgt tatcaggagg cgattctggc agataaacgc aacaagatgc
     2761 tcacgatgtg ggtgacggaa gacgaacacc ggcggttgct tgagcggtgc gacggaaaac
     2821 aactggcggc gtggatgcgc cagacctgcc ttgatgagaa accgacacgc gccggcaggc
     2881 ttccctcgat ttcgcccgcg ctgctgcgtc agcttgccgg catgggcaat aacctgaacc
     2941 agatcgcccg gaaagtgaac gccggtggtg ccggtcatga ccgggtgcag atcgtggcag
     3001 cgctgatggc gatcgatgcc ggtctggaac ggttgcggca tgccgtactg gaaaaagggc
     3061 cggacgatga ttgttaaatt ccatccccgg ggtcgcggcg ggggcgccgg tcctgtggat
     3121 tatcttctgg gtaaagaccg gcagcgtgaa ggtgccagcg tcctgcaggg gaagccggaa
     3181 gaagtccggg agctgattga tgcgtcgccg tatgtgaaaa aatatacctc cggggtgctg
     3241 tcattcgcgg aggcggacct gccgccgggt cagcgtgaaa aactgatggc cagctttgag
     3301 cgggttctga tgcccggact cgataaagac cagtacagca ttctgtgggt ggaacacgca
     3361 gacaagggtc gtcttgagct gaattttctg atccccaata ccgaattact gacggggaaa
     3421 cgcctccagc cgtattacga tcgtgccgac cggccgcgca tcgatgcgtg gcagacagtg
     3481 gtcaacggca ggctggggct acatgatccg aacg
//
