LOCUS       NZ_CP008721             1459 bp    DNA     circular CON 12-JUN-2024
DEFINITION  Escherichia coli strain ST648 isolate Pleural Effusion of Patients
            with Empyema Thoracis plasmid pEC648_7, complete sequence.
ACCESSION   NZ_CP008721
VERSION     NZ_CP008721.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02800875
            Assembly: GCF_001485455.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Gao,Z.
  TITLE     Complete Genome Sequence of Multiple Antibiotic Resistant
            Escherichia coli Isolated from Pleural Effusion of Patients with
            Empyema Thoracis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Gao,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2014) Department of Respiratory and Critical care
            medicine, Peking University People's hospital, Xizhimen South
            Street, Beijing, Beijing 100101, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.3; Consed
            Genome Coverage                   :: 40x; 350x
            Sequencing Technology :: Roche 454 GS FLX; Illumina Hiseq 2000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001485455.1-RS_2024_06_12
            Annotation Date                   :: 06/12/2024 02:11:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,056
            CDSs (total)                      :: 4,958
            Genes (coding)                    :: 4,655
            CDSs (with protein)               :: 4,655
            Genes (RNA)                       :: 98
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 162 of 303
            Pseudo Genes (incomplete)         :: 139 of 303
            Pseudo Genes (internal stop)      :: 50 of 303
            Pseudo Genes (multiple problems)  :: 41 of 303
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP008721.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ST648"
                     /isolate="Pleural Effusion of Patients with Empyema
                     Thoracis"
                     /isolation_source="Pleural Effusion"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC648_7"
                     /geo_loc_name="China:Beijing"
                     /lat_lon="39.93 N 116.35 E"
                     /collection_date="2009-05-24"
                     /collected_by="Dezhi Li"
     gene            complement(join(1233..1459,1..214))
                     /locus_tag="FH07_RS25750"
                     /old_locus_tag="FH07_24965"
     CDS             complement(join(1233..1459,1..214))
                     /locus_tag="FH07_RS25750"
                     /old_locus_tag="FH07_24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000031238.1"
                     /translation="MSKDVERITQINDQGEIIGGFVAVIRPKQKSAFQRHFTMNQDALR
                     ILAKELTGEQFKVLMLMLADLDYENFIQIAQADIAETLGMQKTNVSRAVRALLDVGVIF
                     EGPKVGRSKTYRLNEQFGWKGTVTNHKKALKNGFSVIQGGRI"
                     /name="hypothetical protein"
     Typing          complement(544..624)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.902"
                     /coverage="87.0"
                     /evalue="4.46e-26"
     Typing          739..999
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.13"
                     /coverage="100.0"
                     /evalue="1.84e-104"
ORIGIN
        1 tgaagttttc gtaatccaga tctgcaagca tcagcattaa gaccttgaat tgctccccgg
       61 ttaattcctt agccaagatc cgtaatgcgt cttgattcat ggtgaaatgc ctctgaaatg
      121 ctgatttttg tttagggcgg ataacggcaa cgaagccgcc gataatctca ccctggtcat
      181 ttatctgagt gattctctcg acatccttac tcatctttga accctatttg agttatcaat
      241 aatgataaca gtgttatcat tattgataac tatgtcaact gtaaccctta gattttatta
      301 atgtttttat tctcctttct gtatctttat attaggcgtc taacgcattg attccattag
      361 atatttatcc tagctgctct ttggggtgtc ggggcgaagc cctgacccag tcacgtagcg
      421 atagcggagt gtatactggc ttactcctct gcaagcctcg tcagccatga tttttgtccg
      481 atttctcgct ctccacgttc aacagctcca tcatgttttt gccgtccgtt caccgttaaa
      541 acgcctcaga aatcaggaca ggatgtgcaa atgtaatacc gtcacccgcg acgatattcc
      601 gtttgccatc tggtcagagc ttcgccccga caccccgtat cagtgcgtac gtgctcaatg
      661 tggtttcctg ttctcttccg cttcctcgcc cactcgctca ctaacgttcg gtcgctgggg
      721 ctgcggcgag cggtgccacc tctcgcatgc tgtcggtgat tccgctgttg ttctttcggg
      781 gatgagtcgt cagatagccc ttcaaacgcg ctcagaagct ctcagacgca cgaggcgggg
      841 tttaaatgcc cgtcagggca tggaaggcgc gaaaaacgct tacagagcgt ttcaggaggt
      901 caggcgcgcc gataccccta gccaggggct ttcaggtcgc caggaaccgc gccagccccc
      961 tgcaaagccc atttgcacgg tacaagcagc cactcggttg gtaggtggtc gtagaccgtc
     1021 ggtgttacag cgatgggggg tctgggggtc tccccagacg gtttccggtt gcgctcaagg
     1081 cgctgccgga accgtggcgg ctcggtcgtt ctggagatcg gggagcaaaa acacgcaagg
     1141 gattttgagg cggcaaaaga gcaatgcagg atagacaatc cactagaaat tgcatgcaat
     1201 gttctgtgga ttgtccgcct gccgctggct ctttaaatcc ggccgccctg aatgacacta
     1261 aagccgtttt tgagtgcctt tttgtggttg gttacggtgc ctttccaacc aaattgttca
     1321 ttcagccggt atgtttttga tctgccgact ttaggtccct caaagatcac cccaacatcc
     1381 agtagcgcgc gaacggcgcg actaacattc gttttctgca tccctaaagt ttcagcaata
     1441 tctgcttggg cgatctgaa
//
