LOCUS       NZ_CP008720             1544 bp    DNA     circular CON 12-JUN-2024
DEFINITION  Escherichia coli strain ST648 isolate Pleural Effusion of Patients
            with Empyema Thoracis plasmid pEC648_6, complete sequence.
ACCESSION   NZ_CP008720
VERSION     NZ_CP008720.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02800875
            Assembly: GCF_001485455.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Gao,Z.
  TITLE     Complete Genome Sequence of Multiple Antibiotic Resistant
            Escherichia coli Isolated from Pleural Effusion of Patients with
            Empyema Thoracis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Gao,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2014) Department of Respiratory and Critical care
            medicine, Peking University People's hospital, Xizhimen South
            Street, Beijing, Beijing 100101, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.3; Consed
            Genome Coverage                   :: 40x; 350x
            Sequencing Technology :: Roche 454 GS FLX; Illumina Hiseq 2000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001485455.1-RS_2024_06_12
            Annotation Date                   :: 06/12/2024 02:11:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,056
            CDSs (total)                      :: 4,958
            Genes (coding)                    :: 4,655
            CDSs (with protein)               :: 4,655
            Genes (RNA)                       :: 98
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 162 of 303
            Pseudo Genes (incomplete)         :: 139 of 303
            Pseudo Genes (internal stop)      :: 50 of 303
            Pseudo Genes (multiple problems)  :: 41 of 303
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP008720.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ST648"
                     /isolate="Pleural Effusion of Patients with Empyema
                     Thoracis"
                     /isolation_source="Pleural Effusion"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC648_6"
                     /geo_loc_name="China:Beijing"
                     /lat_lon="39.93 N 116.35 E"
                     /collection_date="2009-05-24"
                     /collected_by="Dezhi Li"
     gene            137..613
                     /locus_tag="FH07_RS25740"
                     /old_locus_tag="FH07_00780"
                     /pseudo=""
     CDS             137..613
                     /locus_tag="FH07_RS25740"
                     /old_locus_tag="FH07_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     Typing          1218..1298
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.73e-35"
     Typing          complement(846..1103)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.0"
                     /coverage="99.0"
                     /evalue="3.93e-111"
ORIGIN
        1 acctgtcgca tccagggtga cacaacgttg tcgcatcctg gatgcgacaa cacgcaacaa
       61 ggtgttgcac agatgtgtca tgatggctta gatttgaacc aacgttagtg catgggattt
      121 tcagagggaa aaaatcatgt ttattgattc agaaaaacga ctgaaacaac tttcagatga
      181 ggcaaagaaa aacaccgagg atctcgaaga agcaaagaag aattcaaggt ttacacaggt
      241 atccccaaaa ggttgggaac gcgttcgaga gctgctgaag gatagccaag gcatatcagc
      301 actgaagctg tactcatttt tagcggagca tatcgatcct acgtgtggcg ctgtcgttgc
      361 ggatcagtaa ttcctagctg aaaaacttgg agttagcaga agcacaatta ttcggtggct
      421 caattactta gaatcaaaaa atgcattagt tagaatcccc gttgctggta aggtttgtgc
      481 gtatgccctc gatccacatg aagtctggaa gggatacaac actacgaaaa accatgcagc
      541 gtttgtcact aaaacactgg tcaacaaaga cggtgatatt cagcgccgaa tcatggccat
      601 gttttcaaat tgagccagcg gcaggcggac aatcaggggc tacgtgttaa cgttttgacc
      661 atgattgtct atcctgcatt gctcttttgc cgcctcaaaa tcccttgcgt gtttttgctc
      721 cccgatccca agaacgaccg cgtcgccacg gttccggcag cgccttgagc gcaaccggaa
      781 accgtctggg gagaacccca gacccccatc gctgtaacac cgacggtcta cgaccaccaa
      841 ccagccgaat agctgcctgt accgtgcaaa taggctttgc agggagctga cgcggttcct
      901 agcgacctga aagcccctgg ttaggggtat cggcgcgcct gacctcctga gagcctctgt
      961 aagcgttttt cgcgccttcc atgccctggc gggcatttaa acccgtttcg tgcgtctgag
     1021 agcttctgag cgtgtttgaa gggctatctg acgactcatc cccgaaagaa caacaacggg
     1081 atcaccgaca acatgcgaga ggtgatcccg ctcgccgcaa gtcgatgacc gagcgtagcg
     1141 agcgaatcga cgaggaagcg gaagagaacc ggaagttaca ttgagcactt acgcacggat
     1201 gcagggtgtc ggggtgaagc cctgaccagg tggggaatgt ctgagtgcgc gtgcgcggtc
     1261 cgacattccc cacctgtccc gatttctgag gcgttttaac ggtgaacgga cggcaaaaac
     1321 atgatcgagc tgttgaacgt ggagagcgcg aaatcgggca aaatcatggc tgacgaggct
     1381 tgcagaggag taagccagta tacactccgc tatcgctacg tgactgggtc agggcttcgc
     1441 cccgacaccc caaaagggcg ttgcgttgcg cgcaacaccc ttgcctagaa tagatctttt
     1501 acgccgattt gtaagttgtt gtttttttag tgtgtttttt gttt
//
