LOCUS       NZ_CP007714             6076 bp    DNA     circular CON 21-APR-2024
DEFINITION  Acinetobacter baumannii LAC-4 plasmid pABLAC2, complete sequence.
ACCESSION   NZ_CP007714
VERSION     NZ_CP007714.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02709859
            Assembly: GCF_000786735.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii LAC-4
  ORGANISM  Acinetobacter baumannii LAC-4
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Ou,H.Y., Kuang,S.N., He,X., Molgora,B.M., Ewing,P.J., Deng,Z.,
            Osby,M., Chen,W. and Xu,H.H.
  TITLE     Complete genome sequence of hypervirulent and outbreak-associated
            Acinetobacter baumannii strain LAC-4: epidemiology, resistance
            genetic determinants and potential virulence factors
  JOURNAL   Sci Rep 5, 8643 (2015)
   PUBMED   25728466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Xu,H., Chen,W. and Ou,H.-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2015) Department of Biological Sciences,
            California State University, Los Angeles, 5151 State University Dr,
            Los Angeles, CA 90032, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: GS De Novo Assembler v. unknown
            Genome Coverage                   :: 43.5x; 255.6x; 408x
            Sequencing Technology :: Roche 454 FLX+; Illumina Hiseq2000;
            Illumina Miseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000786735.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 01:04:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,838
            CDSs (total)                      :: 3,744
            Genes (coding)                    :: 3,595
            CDSs (with protein)               :: 3,595
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 56 of 149
            Pseudo Genes (incomplete)         :: 89 of 149
            Pseudo Genes (internal stop)      :: 19 of 149
            Pseudo Genes (multiple problems)  :: 12 of 149
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP007714.1.
            Source bacteria/DNA available from Dr. H. Howard Xu, Department of
            Biological Sciences, California State University
            (hxu3@calstatela.edu).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Acinetobacter baumannii LAC-4"
                     /mol_type="genomic DNA"
                     /strain="LAC-4"
                     /isolation_source="unavailable"
                     /host="Homo sapiens"
                     /db_xref="taxon:1455315"
                     /plasmid="pABLAC2"
                     /geo_loc_name="USA: Los Angeles County, California"
                     /lat_lon="34.0500 N 118.2500 W"
                     /collection_date="1997-01-02"
                     /collected_by="Public Health Laboratory"
     gene            40..573
                     /gene="aadB"
                     /locus_tag="RR41_RS19080"
                     /old_locus_tag="ABLAC_p200010"
     CDS             40..573
                     /gene="aadB"
                     /locus_tag="RR41_RS19080"
                     /old_locus_tag="ABLAC_p200010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(732..1399)
                     /locus_tag="RR41_RS20230"
                     /pseudo=""
     CDS             complement(732..1399)
                     /locus_tag="RR41_RS20230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /name="Abi family protein"
     gene            complement(1532..2017)
                     /locus_tag="RR41_RS19090"
                     /old_locus_tag="ABLAC_p200040"
     CDS             complement(1532..2017)
                     /locus_tag="RR41_RS19090"
                     /old_locus_tag="ABLAC_p200040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            complement(2126..3940)
                     /locus_tag="RR41_RS19095"
                     /old_locus_tag="ABLAC_p200050"
     CDS             complement(2126..3940)
                     /locus_tag="RR41_RS19095"
                     /old_locus_tag="ABLAC_p200050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3930..4337)
                     /locus_tag="RR41_RS19100"
                     /old_locus_tag="ABLAC_p200060"
     CDS             complement(3930..4337)
                     /locus_tag="RR41_RS19100"
                     /old_locus_tag="ABLAC_p200060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5339..6025
                     /locus_tag="RR41_RS19105"
                     /old_locus_tag="ABLAC_p200080"
     CDS             5339..6025
                     /locus_tag="RR41_RS19105"
                     /old_locus_tag="ABLAC_p200080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     ARG             41..570
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             2..573
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="107.34"
     Typing          complement(2130..3940)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attactgaca ttctgggagg gcttactatg ttaggccgca tggacacaac gcaggtcgca
       61 ttgatacacc aaattctagc tgcggcagat gagcgaaatc tgccgctctg gatcggtggg
      121 ggctgggcga tcgatgcacg gctagggcgt gtaacacgca agcacgatga tattgatctg
      181 acttttcccg gcgagaggcg cggcgagctc gaggcaatag ttgaaatgct cggcgggcgc
      241 gtcacggagg agttggacta tggattctta gcggagatcg gggatgagtt acttgactgc
      301 gaacctgctt ggtgggcaga cgaagcgtat gaaatcgcgg aggctccgca gggctcgtgc
      361 ccagaggcgg ctgagggtgt catcgccggg cggccagtcc gttgtaacag ctgggaggcg
      421 atcatctggg attactttta ctatgccgat gaagtaccac cagtggactg gcctacaaag
      481 cacatagagt cctacaggtt cgcatgcacc tcactcgggg cggaaaaggt tgaggtcttg
      541 cgtgccgctt tcaggtcgcg atatgcggcc taacaattcg tccaagccga cgccgctttg
      601 cggcgcggct taactcaggt gttaggaaac actgctaccg ttatcggatt tactgcttta
      661 ctactaacct tgttaattca acatttgcaa aatagtctga cctttcgatt gtttggggtg
      721 aaattcttat cttaaaaatc taaatattct tttagatcta atgcagattg ctcgataaaa
      781 tcgtatgtat taatacaacg acagtaaata aattttgtta aaacctctaa atcattttct
      841 agattacggt taaaaataat cggttcatga tgagcaatcc tgtttcttaa ttcacgtaat
      901 tgatctgtcc aatcgtaaaa aatttgcctg ttttcaactg ttgcattagg aaaaatagaa
      961 ttaaaacatc cctgccaaat acgttcttca taattagcca taagcatctt ttgccagaat
     1021 acaaatttaa gctcagggat cattttacta ggcgaatctg ggaacttctc tttagcacta
     1081 tcaaacatac ttttagggct atacctccct ctacggctaa ggcttcgaat aaaagcttca
     1141 ttttcatgcc aactatttgt ttcattacac ctaaaagata tttctattgc ctgaacaaca
     1201 gcatttctca gaacaacttc aaaataattt aataagtaaa aaacttattg gaaattatta
     1261 aattacgttc ataaagcatc aatacatcag atttttcata tggttcacct ttatctttat
     1321 agaaattttc atacttagat aagcgtggta tagataaagc ctgctctatc ttaagcactg
     1381 catcatttga caattccacc gtatataccc taatatatag ttgtaagttt gagagactgc
     1441 ggatctaaga tcttccctcc caagcgctaa gattcaaaaa agcctttcat taacgtgaaa
     1501 ggcttttttc tttttttggt caattaaaag cttacatatt gattgtaatt gaaatatttt
     1561 cttgagggac atcaaaataa ttagctaata gtctcttagc ttcaggaata gatattttat
     1621 ccagtgtttt agaagaatta tcttcattta atttggtaaa tttcagctca ttcaaatcga
     1681 tattcatctc ttctaatgaa ctataagaaa ctggattccg attaccatcc cacatattgg
     1741 gaatagatat atcagcatat tcggagatat ttattatcca tctaccatct gcatcttctt
     1801 cttgagattg gctgatacta gatattttgc caactaaaaa tgcagtgtga tgatcagcct
     1861 ctggttctcc ccaagattgt ttcctatttt gcacacaaac tagataatcg cactttcctg
     1921 cattatgagc atttagtctc caagactgac taccaccttc ttttaaaatt ctagtctttc
     1981 cacgagcagt tagtactact acacagttat tattcataac accaccaaaa tatataaaca
     2041 agcctatact acattcaata tagctaaatt taaacattag tacaagctaa tactaagaaa
     2101 taaacaccaa catactagaa aataattaca tcccaaaatc catacctcga tcttgcttag
     2161 gtcgaggttt ttctatctct tgtttacgct cattcatctg gacgagattt ttttcaatat
     2221 ctcggatgtt tcgagcaaat tctgctgcgc tttggctact tctgacgaga cagaatgctg
     2281 tgtggttgag attctctcca gaatttcttc gttgttggct gtgataaggc tcatctctcg
     2341 gactttgcca ttgatctgct cggtatagct tgctgtactt cgttctagct cgtgaaattg
     2401 cttctgcatt gtcgctaatg atttctgtgc ttgctgtaag ctttgctgat cgctcttgat
     2461 tgtctttgat tgtgctgaca aggcttggat aattttggtt tgcattgtgt ctaaagcttg
     2521 ctgttgcttc tgcaattgca cttgttgttg cattaattgt tgctgttgtt cttcgatgat
     2581 tctcaataat tgtgtttcta attcggtcat cgtttaatac cccattggaa tcatgtagcc
     2641 gttctgccat tcgcctgcgt tcttgttgat ctgaatacat aggcgctgtg ctttgctgtc
     2701 tggtcgcttg catgtgctga tcgtcaaact gcctcccgat cggttcaaat ggtcgaccct
     2761 catttgcttc tcgttgatca cggtatttag cgtctgcatc tcgctgtact tgtttttggt
     2821 gacgagacca agcgagaaaa ttgctataag caaaataact gctactgaaa tcagtcccat
     2881 attcaaagtg gtaagttttt tctgtaccgc ttgattctgg gcttgttgtt cttggctctg
     2941 tatttgctcg tttaattgcg ttaaatggct caatttcgat tgcagcaagg tttgatacag
     3001 gcttaactgc ttctcgaccg tctgggcgtt gttttgaatg ctctgggcta ttttctcgtc
     3061 cgactgttgg atagcggtcg cagtgatatt ggctcttatg ttcaagctgt ctctgatagt
     3121 ctgtgacgtt tgttcgatac cgctcagaag ttgttcctct gtattcttca attcgttgtt
     3181 gtacttcttg tctatattcg ccagtagctc taaagccttg ctcataaatc tcgccctcta
     3241 aacggatggg acgttttgcg ccctcatatg ggttttcaat ggatatggag ttttttactt
     3301 gtcgagtgac attgatttga ttcgattcaa gccattgcac caattcttgc cgatcggcaa
     3361 catcaccatt ggcaaccgct cggtaaacac gttgatgtaa ctgcgcttta aaatccttaa
     3421 tatctttggg taggttcttt ttatttaaaa atagctgtcg atgctcagga tcatcggggt
     3481 catacagtga atgttcggta ttagtgatct gcttaaatag atcgactcga tctaaatcga
     3541 ctggcgcata gaatggctgt aggcgtttgc ctgttgaaag ctcaacatta ggaatcacaa
     3601 aattaagctc aagccttgtc tcacctgtgt cctgattaat tttgtcttgg tgctgtaccc
     3661 ataaaatttg atattggtct ttatctagtc ccgggaataa acattcctca aaattttgca
     3721 tgattttctg tttatcctgg tcagataaat catgctcata aaaggacaaa caaccactgg
     3781 tatatttttt ggcaaaagga ctgctatcaa tgagttgtgc tgtcagttct acatcaccat
     3841 tgagcacttg agcatgttca cgctctcgat cttcacctaa caaataatcg agacagcctt
     3901 ttgataaacc tgaaccatgt ctaaagaaat caacgatcat ctggacgtaa ctcccgaagc
     3961 tgctgatcta tggaaatcaa caaatgaagt aacttcactt tgtcaaaagt acctacccct
     4021 gcaatatcgg tattcactgc tttagcaatc tgattcacat tgttgccaat ccgaccaagc
     4081 tctaaaatta atgctcgctc tgtcttgcta tacgaagatt caacaacctt tgttgcttta
     4141 attttttggt catcttctgc tttggaatta tgaattttag tcgctttctc tactgctgct
     4201 tgtctaagca actttgcaat cgaaccataa ggattattgg aatcgagcaa ttgatattct
     4261 gattctgaaa gtctgacttt aaaggttcgt tctcgaactg ctttgctctg tttgttttct
     4321 tgttcctgtc ctgacatttc aatctaccct gctttggctt ttgtaagctt taaattggaa
     4381 aatctttgat ttttctgttt aaagcttttc gggggtatcg ggggtgaagc ccctgatcaa
     4441 ggtcacagac tatcactgtt taacgaagtt aaataggggt agctgtgtat ccttgcttat
     4501 ttaaaaatgg acgagtgaaa ttcaataaaa actaatcaaa tgacacggta tataaactct
     4561 agtaggtatt ggcatgaatc ttgtaaaaac aaatgcgctc aactctttct caccgtcccc
     4621 acatccctcg ctttgctcgg gctgatggtg aacgttgaga gagtttcggc aatactttcc
     4681 ctaagaggct ttgatttaaa aaaatcaaat atcctcttaa gtattgccga ttaacctgta
     4741 attgggctga cggagccacg catcggtcgt atccgcacat ggttttgctg accatgcaga
     4801 caaggactaa aggttttgca ccttctccca cgcgctccct gtcgaagtat ggtggtatct
     4861 ctgcgaggat tactccaaca tgcaacttaa ccaacctata caactacttc caacgctccg
     4921 cctatgtgag atcccctgta agcggtttag ccttgcgact atcgctacga tgcaccagtg
     4981 acacttattc cctccacatg caacgcatat cttccttaaa aaaggcgcac agaagtagat
     5041 aaagctcgac cgttaggtgc ttatttgatg atttaaactg ctgaattaac gaaggacttc
     5101 aaattgaggc ttgtcaaact gttctaattc tttaaaatag atagtggtac tagcctttaa
     5161 ggtgaatatc agtatgagtt gcaagctctg ccgatctttc gttaattgat gttgcaagca
     5221 tcaaataact gtacccatcg aatttaaaac taatggacag atttcgcaag agacctgtcc
     5281 attatttttt tgattacacc taagaattaa gctgaaactc ctattctttg gactattaat
     5341 gtcaaaactt cctagatttc ataacaagca catcgaaaaa cgtaagtatt caaatagcct
     5401 cctagccaaa tctatttctt ctaaagtacg tttctctaat aaaacatttt ttaatgttaa
     5461 tttgagagga tcccgcctac gatcatgttc attcaaaaat ataaaaattg ttttatctga
     5521 tttttcagga gtaatcttaa ataagaatag gtttacgaat gttcacttca aaaagtgcgt
     5581 attttatgct gctattttta gagattgtcg ctttaaaaat tgcacatttg aaaaatgcat
     5641 ttttattaat acaaatacag aaagcctaaa tcactcgata ttagactgtt ctaaaaaata
     5701 taatttttac gaaatgaaaa ttcctgatga cttaaaagat caactaacag aatatagaaa
     5761 tattccaata ctgcaaaaaa accgtttact tcatctaaaa gggggaagaa tcaataccgc
     5821 aaccttattc attctcctat ccaaaatatc caaatacaag ctgctaaatg gtcttaaaaa
     5881 aatttatatt gaggaacaaa gtaaagtaaa aatacttagc actttcaaac tccttgaaga
     5941 aataaagaac ctagttgaca ctaacaccgc attcaaacag aataatggtg ctctgcccca
     6001 tccaatcgaa aggttggata gttaagacaa tcactgggag ctcactattg gatgtaacaa
     6061 ttcaattaat agtcta
//
