LOCUS       NZ_CP007713             8006 bp    DNA     circular CON 21-APR-2024
DEFINITION  Acinetobacter baumannii LAC-4 plasmid pABLAC1, complete sequence.
ACCESSION   NZ_CP007713
VERSION     NZ_CP007713.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02709859
            Assembly: GCF_000786735.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii LAC-4
  ORGANISM  Acinetobacter baumannii LAC-4
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Ou,H.Y., Kuang,S.N., He,X., Molgora,B.M., Ewing,P.J., Deng,Z.,
            Osby,M., Chen,W. and Xu,H.H.
  TITLE     Complete genome sequence of hypervirulent and outbreak-associated
            Acinetobacter baumannii strain LAC-4: epidemiology, resistance
            genetic determinants and potential virulence factors
  JOURNAL   Sci Rep 5, 8643 (2015)
   PUBMED   25728466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8006)
  AUTHORS   Xu,H., Chen,W. and Ou,H.-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2015) Department of Biological Sciences,
            California State University, Los Angeles, 5151 State University Dr,
            Los Angeles, CA 90032, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: GS De Novo Assembler v. unknown
            Genome Coverage                   :: 43.5x; 255.6x; 408x
            Sequencing Technology :: Roche 454 FLX+; Illumina Hiseq2000;
            Illumina Miseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000786735.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 01:04:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,838
            CDSs (total)                      :: 3,744
            Genes (coding)                    :: 3,595
            CDSs (with protein)               :: 3,595
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 56 of 149
            Pseudo Genes (incomplete)         :: 89 of 149
            Pseudo Genes (internal stop)      :: 19 of 149
            Pseudo Genes (multiple problems)  :: 12 of 149
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP007713.1.
            Source bacteria/DNA available from Dr. H. Howard Xu, Department of
            Biological Sciences, California State University
            (hxu3@calstatela.edu).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8006
                     /organism="Acinetobacter baumannii LAC-4"
                     /mol_type="genomic DNA"
                     /strain="LAC-4"
                     /isolation_source="unavailable"
                     /host="Homo sapiens"
                     /db_xref="taxon:1455315"
                     /plasmid="pABLAC1"
                     /geo_loc_name="USA: Los Angeles County, California"
                     /lat_lon="34.0500 N 118.2500 W"
                     /collection_date="1997-01-02"
                     /collected_by="Public Health Laboratory"
     gene            946..1869
                     /gene="repM"
                     /locus_tag="RR41_RS19040"
                     /old_locus_tag="ABLAC_p100020"
     CDS             946..1869
                     /gene="repM"
                     /locus_tag="RR41_RS19040"
                     /old_locus_tag="ABLAC_p100020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282204.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_000845851.1"
                     /translation="MKTELIVKDNALINASYNLDLVEQRLILLAIVEARESGKGINAND
                     PLTVHAESYINQFGVHRNTAYQALKDACDDLFARQFSYQSLSEKGNIINHKSRWVSEVA
                     YIDNEAVVRLIFAPAIVPLITRLEEQFTKYEIQQISNLTSAYAVRLYEILIAWRSTGKT
                     PLITLSDFRQKIGVLDTEYKRMYDFKKYVLDIALKQVNEHTDITVKVEQHKTGRSITGF
                     SFSFKQKKSVTKSAKSIGVSEDITITLTDAQRYSFASKLSELPEMGKLSQGTESYEQFA
                     VRIADMLKQPEKLKELTPLLRKVGFQ"
                     /name="repM"
     gene            1937..2482
                     /locus_tag="RR41_RS19045"
                     /old_locus_tag="ABLAC_p100030"
     CDS             1937..2482
                     /locus_tag="RR41_RS19045"
                     /old_locus_tag="ABLAC_p100030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000736775.1"
                     /translation="MKKLSVSELAKLYGYSRQAIYAHINKGNLSKGSDGLIDFAEAIRV
                     FGEPQKKDTNVNQSQSINSQNLTEVDLLKRQVDILEKQLNQALFRENQSLERESFYQDQ
                     IEAMQRLLEAPKANMTTFTDQEQSQANVAQVQPEPNIDDPKHDGLTTPENKRIPVPEHV
                     EPEPKKRGFWSRFFLPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            2820..3137
                     /locus_tag="RR41_RS19050"
                     /old_locus_tag="ABLAC_p100040"
     CDS             2820..3137
                     /locus_tag="RR41_RS19050"
                     /old_locus_tag="ABLAC_p100040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000900860.1"
                     /translation="MLGKNHHFLKKFSISDAAIAYKKPRSTILKYIKSGDFELDYNKLI
                     EYSQLVRKFGELTYPIQDEREQQIKKLKDEVMHLKKQLNFFKYTSISNKNRLLISHKHS
                     I"
                     /name="hypothetical protein"
     gene            3665..4021
                     /locus_tag="RR41_RS19055"
                     /old_locus_tag="ABLAC_p100050"
     CDS             3665..4021
                     /locus_tag="RR41_RS19055"
                     /old_locus_tag="ABLAC_p100050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269904.1"
                     /translation="MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSNKKRFYTEMLAIADEQ
                     YALHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4014..4316
                     /locus_tag="RR41_RS19060"
                     /old_locus_tag="ABLAC_p100060"
     CDS             4014..4316
                     /locus_tag="RR41_RS19060"
                     /old_locus_tag="ABLAC_p100060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001129974.1"
                     /translation="MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            4309..4518
                     /locus_tag="RR41_RS19065"
                     /old_locus_tag="ABLAC_p100070"
     CDS             4309..4518
                     /locus_tag="RR41_RS19065"
                     /old_locus_tag="ABLAC_p100070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069475.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCEYFS
                     HCKRPSYYIDIFDNAYWLKYYEIA"
                     /name="hypothetical protein"
     gene            4646..7318
                     /locus_tag="RR41_RS19070"
                     /old_locus_tag="ABLAC_p100080"
     CDS             4646..7318
                     /locus_tag="RR41_RS19070"
                     /old_locus_tag="ABLAC_p100080"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_148307306.1"
                     /translation="MNILQRKSSMVLYAFEQSIGDFVLNYKDLNIGSRLIEDITEREIS
                     KGRSFDPNQIKDVVEATYLGELFQIAIDLTSNDSSHDLVKYLYSLFHDFEIHLVRNAIS
                     HPNRQFLDFFWYRLAAVATDPVIDILGLDKVKKAFISANEGILDDPPDDWLNKVVWEIP
                     NNLPSTFEHAVTGLIGRNKERKQLETYMSQPRVSTVALVAPGGVGKTALALDLLHYIVH
                     RPSAVNRLDGVVFVTLKTEKLTAQGIQQLTAIQTIAEVKNKIVESINDIFDEDLKNFED
                     VASLYDQKRILLCIDNLETVLREEQILFDDFNNTLPGGWKVLITSRITVENTKTLSLHP
                     LDQKSGEILARKYQESRGISTLENHDYEKISKACFFNPLAIRLTIDLINLGRDIPTSIS
                     EAQKNIAEFSYNNLIEALKPTSIQILEAIFVENVSTRASLCHVLEKDLDEISDSVGELS
                     RTSLIVKASQKDIETYSLNDSIKDLLLLDPKNLEVRNKVQNKLLEQKNQVREIDFHQEK
                     RQYPIWHRDNIPLDTPEGLKILVKNVNMAVRRKGETSTLSELIRKLRDAENIYKTYAIY
                     YKSLAKLLDKLSDSHGQKDALLKGLAIDDTNPNILYMLARYYADQGDYEASTKQYQKLI
                     ELGWIQDNEENIEFSRTIYNGYFLNLLYSKEYQRILDETKKWKEDENFSPLLGVYRATA
                     WKRTIELYGAEESDKIESAINSSLAIFDEVFRKYGYSVEACKQATKVFEEIIFYANNPL
                     KSDEKLKIFLEKTKKNITEISNTYLDYDWTNLYKKLSSIQISDNPFYNLNRSGYKSPSQ
                     MYADGSKEGDFIFVKIETIRNNYLFSSDKNQTQYFVHSNQYRGDVNWYKLKEGELLKVH
                     PLPPDSSQSAIKVKAAFSC"
                     /name="ATP-binding protein"
     gene            complement(7392..7583)
                     /locus_tag="RR41_RS19075"
                     /old_locus_tag="ABLAC_p100090"
     CDS             complement(7392..7583)
                     /locus_tag="RR41_RS19075"
                     /old_locus_tag="ABLAC_p100090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102616.1"
                     /translation="MSTELVNSANIISFPKPCAFCESTEHVQLFAGLMLCRSCQENIKI
                     TNPDLFATNDQIQQKAQD"
                     /name="hypothetical protein"
     Typing          947..1550
                     /name="rep_cluster_1254"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.835"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tagttatatt agtttttatc catttttttg aaacagtttc tatatgataa aaaaacctat
       61 aaaaaccata tctagcaaag gtttgagggt tatcatcttt agatgcgtgg tgtgtgacaa
      121 aaaaatcccg gcatgtgccg gattctggat tagaaaattg gctaaagtga cgtaggactg
      181 gtgcttggtt ttacatggaa aaaagtattt attttctggt ttataaaaac gtaaaaagat
      241 cagtttttgt tcattcatcc aggttaaaaa tttcaaccta aaactttaat tatgaaaagc
      301 ttcacagaaa gcattcaaat gcgatttaag agcctttatc taaaaaacat agatcttata
      361 gcgaaaaaca gaaaacagct caaaaaacgc aaaatagagt gaagtaaaga gatgttttga
      421 ctttagatag ctgcatagag cgagtgtcta cgagcgaact atcaaaattt gcgtctagac
      481 tctctgaaaa acattttttt gccctcttta gcctaagaaa gcttaatttt catgcagaaa
      541 tttgctcctg gaccgagcgt agcgagaaaa aaagctcatg agcgaagcga attccgagtt
      601 gcttttgctt tttcttaaag tcacgcaagt attaaccaaa aaattgcccc gacgaactga
      661 gcgaaagcga agttcaatag agtttgagcg aagcgaaaac caagggcaat ttttcatgac
      721 ctgagctttt aaatattttt aaatctttta aatgctttta gacatgctga aagcctttat
      781 ttataaaatt cttagagact ataaaacgag gtttaccttg cattaaaacg aggtttacct
      841 tgcattaaaa cgaggtttac cttgcattaa aacgaggttt accttgcatt aagcgagttt
      901 atgatatagc ctcgtcttat tgaattaaaa atataacctt ttcatatgaa aacagaacta
      961 atagttaaag ataatgcatt aattaatgcc agttataacc ttgatctagt cgagcaacgg
     1021 ttaattcttc tagctatcgt tgaagcaagg gaatcgggta agggaataaa tgctaatgat
     1081 ccattaacag ttcatgctga aagctacatc aatcaatttg gtgtacatcg aaacacggct
     1141 tatcaggcat taaaagatgc ttgtgatgat ctatttgcaa gacaattcag ttatcaaagt
     1201 cttagtgaaa aaggcaacat tattaatcac aagtcaagat gggtgagtga agtcgcttat
     1261 attgataatg aagcggttgt tagacttatt tttgctcccg ctatcgtgcc tttaattaca
     1321 agactagaag aacaatttac aaagtatgaa atacaacaaa taagcaattt aacaagtgct
     1381 tatgccgttc ggttatatga gatattgatt gcatggcgta gtacaggaaa aacgcctctt
     1441 ataactttgt ctgatttcag acaaaaaata ggtgtactcg atactgaata caaacgaatg
     1501 tatgacttta aaaaatatgt cttagacatt gcattaaagc aagtaaatga acataccgac
     1561 attactgtta aagttgaaca gcataagact ggtagatcaa ttacaggttt ttcatttagc
     1621 tttaaacaaa aaaaatcagt gacgaagtca gctaaaagta taggtgtaag cgaagatata
     1681 acgatcactt taacagatgc acaacgctat tcatttgcga gtaaattgtc agagcttcca
     1741 gaaatgggaa aactttcaca aggcaccgaa agctatgaac aatttgctgt acggattgca
     1801 gatatgctaa aacagccgga gaaattaaaa gaacttactc cattacttcg aaaagttggc
     1861 tttcaataaa tgatgtcaat tgacattgta aagttgacag tcaagtatta acacaaattg
     1921 aaaactggtt tacataatga aaaaactgtc agtttcagag ttggctaagc tttacggata
     1981 ttcacgacaa gccatatatg cccatataaa caaaggaaat ttatctaaag gatctgatgg
     2041 attaattgac ttcgcagaag ccataagagt ttttggggaa ccacaaaaga aagatactaa
     2101 tgtcaatcaa agtcaatcaa ttaacagtca aaacttgaca gaagttgacc tattaaaacg
     2161 tcaagttgac atactggaaa aacagttaaa tcaagcactg tttagagaaa atcaatcact
     2221 agaacgtgaa tcgttttatc aagatcagat cgaggccatg cagcgcttac tagaagcacc
     2281 aaaagccaat atgactacgt ttaccgatca agaacagtcg caagcgaatg tagctcaagt
     2341 acagccagaa cctaatattg atgatcctaa acatgacgga ttgactactc cagagaataa
     2401 gcgcattcct gttccagagc atgttgaacc agagcctaaa aagcgtggct tctggagtcg
     2461 tttttttctt ccatatggtt aacttaggct aaataaaacc gtggaacatt gctcaataaa
     2521 taagcgtgga acgtgaaaaa tcataaaaaa aacccacctt ggggaagatg ggctataaac
     2581 tagaaactac agcattgatt tctaaggaaa attataacac atttcgtata aggtgtatta
     2641 tgttaataat ataaattttt aaaagcatat tgatttaata atagaaaaat tatcagagta
     2701 gggcatgtgt aaagtaaacg ttcgattatt aaccaataat gattaaagat ttatatcttg
     2761 tatgtgttaa ttttattaac atatatatac gaaattaata atgccttaag tgaaatagta
     2821 tgctaggtaa aaatcatcat tttttaaaaa aattttcgat cagtgatgct gctatagcgt
     2881 ataaaaaacc tcgctcgact atacttaaat atataaaaag cggagatttc gaacttgact
     2941 acaataaatt gattgaatac agtcagttag ttagaaaatt tggtgaactt acttacccta
     3001 tacaggacga aagagagcaa caaatcaaaa aactaaaaga tgaagttatg catttaaaaa
     3061 aacaacttaa cttttttaaa tatacttcaa tctcaaacaa aaatagacta ttaatttctc
     3121 ataaacattc catttaacat aatgtgcgtt atccgaaatg gccctttaag tttactttaa
     3181 gatcaacaac ttacattgag ttaaacccct ggaacgatct cgttcaaccg atttagaaat
     3241 aaatcggttt ttttatgttc aattctgata cttcttgcca ataaaattgg ttaaaaaatg
     3301 atcaaaaata ccaattccat agagtttgta agcctttttt acccaaaaga atccacaatt
     3361 tatgcggata accattggga taaattttga ttattttttg tacgttttgg cttacagcgt
     3421 ttcacgaaat tgtctgtatc tatcccccga tgaagctctt atgatcgatc tatatggaca
     3481 ggatgttcaa tgacttaaaa caacaataat aatcagtcag gcaccatgac catcaggtag
     3541 tcagccctac ctatgcgctc cataccgaaa aatcccgaca tgacgtcggg attttttatt
     3601 gccatttgca aagtcattca atcagattta atatatagac tgtaatctat attgtgattg
     3661 taatatgtgg acagtcatta caacggatct gtttaatgaa tggcttgaac agcaagatga
     3721 agcaacacaa gagaaagtct tagctgcatt ggttgttcta caacagcaag ggcctagttt
     3781 aggtcgtcct ttagtagata ccgtctacga ttctaaattt accaatatga aggaactacg
     3841 tgttcagcat cgtggtaaac cgttaagggc attttttgca ttcgatcccc taagacaagc
     3901 gattgtgctg tgtattggtg ataagagtaa taaaaagcgt ttttatacag agatgctagc
     3961 aattgcagat gagcaatacg cacttcatct ctcaacttta ggagatcaat ctaatggcta
     4021 agagcttaca agaattattg gctagtcgct ctccagagag tcaagctcgt attcaaaaaa
     4081 tggctgatga attactttta gaaagtcagc ttcaccttat ccgagaagag ttagaaattt
     4141 cccagaaaga actagctgca acccttggaa ttaaacagcc gtcattatca gcaatcgaaa
     4201 atcgtggtca tgatcttaaa atttcaacga tgaaaaagta tgttgaagca atgggtggaa
     4261 aacttcgtat tgatgtagag cttccaacag gaaaacacat agggttcaat gtctaataat
     4321 aaacctttga aaaatagtac taaattactt gttaacttag acaaatttat atttttagtc
     4381 aatgctgccg atagcctaga agaaattgaa attattagag atttatgctg tgaatatttt
     4441 tcacattgta agaggccaag ctattacatt gatatttttg acaatgctta ttggttaaaa
     4501 tattacgaaa tagcttaatc cttctagatt cgcataagag gttttatgtt aaataaaatt
     4561 taaataatga ttagttacta tttgatttta aattaaaaaa tgttatatcg attagttgag
     4621 acttaattcc ttagaaaatt taaatatgaa tattctacaa cgtaaatcct ccatggtctt
     4681 atatgctttt gagcaatcaa ttggagattt tgttttaaat tataaagact taaatattgg
     4741 tagtcgtttg attgaagaca ttacagaacg ggaaatatca aaaggtcgat cttttgatcc
     4801 gaatcaaatt aaagatgttg ttgaggcgac ctacttaggt gagttatttc aaatagctat
     4861 agacttgact agtaatgatt cttctcatga tttagtcaag tatttatatt cattatttca
     4921 tgattttgaa atccatttag tcagaaatgc gattagtcat ccgaatcgac agtttttaga
     4981 ttttttttgg tacagattag ctgcagtagc aactgatcct gtaatagata ttcttggatt
     5041 agacaaagtt aagaaagctt ttatttctgc aaatgaaggt attttggatg atcctcctga
     5101 tgactggctt aataaagtag tctgggaaat tccaaataat ttaccaagta cttttgaaca
     5161 tgctgtaaca ggattaattg gtagaaataa agaaagaaag caattagaaa cttatatgtc
     5221 tcaaccccga gtttctactg ttgctctagt cgccccagga ggagtaggaa aaacagcctt
     5281 agctttagat cttttacatt atattgtcca tagacctagt gcagtaaatc gtttggatgg
     5341 agttgttttt gttactttaa aaactgagaa attgacagct caaggtattc aacaacttac
     5401 agctattcaa actatcgcag aagttaaaaa caagatagtg gaatctataa atgatatttt
     5461 tgatgaagat ttaaaaaatt ttgaggatgt ggcctcgtta tatgatcaaa aaagaatcct
     5521 gctatgtatt gataatttag aaacagtatt gagagaagaa caaattcttt ttgatgattt
     5581 taataataca ctaccaggtg gatggaaggt tctcatcact agtcgaataa cagttgagaa
     5641 tactaaaact ttaagtttgc atcctcttga tcaaaaaagt ggggaaattt tagctagaaa
     5701 atatcaagag tcccgaggta ttagtactct agaaaatcat gattatgaaa aaatatcaaa
     5761 ggcttgtttc ttcaatcctt tagctatacg tttaaccatc gatttaatta atttaggacg
     5821 cgatattccc acttctatta gtgaggcaca aaaaaatatt gctgagtttt cttacaataa
     5881 tttaatagaa gccttaaaac caacatcaat acagatttta gaagcaattt ttgtagaaaa
     5941 tgtatcaaca agggctagct tatgtcatgt tctcgaaaaa gatttagatg agatctctga
     6001 tagtgttggt gagctatcta ggacaagttt aatagttaaa gcatctcaaa aagatatcga
     6061 aacttatagc ttaaatgatt cgatcaaaga tctactttta ttagatccaa aaaatttaga
     6121 agttagaaat aaagttcaga acaaattact agagcaaaaa aatcaagttc gtgaaattga
     6181 ttttcatcaa gagaaacgtc aataccctat ttggcataga gataatattc ctttagacac
     6241 tccagaagga cttaaaattc tagttaaaaa tgtaaatatg gcagtgcgtc gtaagggaga
     6301 aacaagtacc ttatctgaac taattagaaa gttaagagat gctgaaaata tttataaaac
     6361 atacgcaatt tactataaat ctttagctaa attattagac aaactttctg atagccatgg
     6421 tcaaaaagat gcacttttaa aaggtttggc tatagatgat actaatccaa atattttgta
     6481 tatgttagcg agatattatg cagatcaggg agattatgaa gcttcaacta agcagtatca
     6541 aaagttaata gaactaggat ggattcaaga taatgaggaa aatatagaat tttctagaac
     6601 catatataat ggttatttcc taaatttact ttactcgaaa gaatatcaac gaattctaga
     6661 tgaaactaaa aagtggaaag aagatgagaa tttctcccct ttattagggg tctatcgagc
     6721 tactgcttgg aaaagaacta ttgaattata tggagctgaa gaaagtgata aaattgaatc
     6781 agctattaat agttcattag ctatttttga tgaagtattt cgaaagtatg gttacagtgt
     6841 agaagcatgt aagcaagcta ctaaagtatt cgaagaaatc atattttatg caaacaaccc
     6901 ccttaaatca gatgaaaaat taaaaatttt tcttgaaaaa accaaaaaga atatcacaga
     6961 aatttcaaat acatacttgg attatgattg gacgaactta tataagaaac taagttctat
     7021 tcagatttca gacaatcctt tttataacct taaccgatct gggtataagt cgccaagtca
     7081 aatgtatgct gatggttcaa aagagggtga tttcatattt gtaaaaatag aaactattag
     7141 aaataactat ctattttctt cagataaaaa tcaaacacaa tattttgtgc attctaatca
     7201 atatagagga gatgtaaatt ggtacaagtt aaaagaagga gagttgttaa aagttcatcc
     7261 tctaccacca gattctagtc aatcagcgat aaaggtcaaa gcagctttta gttgctagat
     7321 tgagtcaaaa acttagaaag tttagttata ttttcttaaa ttaacataat acaccttata
     7381 cgaagcccag gttaatcctg ggctttttgt tgaatctgat cattggtagc aaacagatca
     7441 ggattggtaa ttttgatgtt ttcctgacaa ctcctgcaaa gcatcagccc agcaaaaagt
     7501 tgtacatgtt ccgttgattc acagaaggca catggcttag gaaaagagat gatattggct
     7561 gaattgacta attctgttga cataagaagt aaccttggat tgtacatatt tatttttaat
     7621 aaatttcaat actttatacc taatttagcc actaaaattt gtacattatt gtataatcca
     7681 tgtgtacata ttatttttta taattttcat tcacttagac ccaaaatagt atttattttt
     7741 gtacaacacc atgtacagaa caataatcat ataaatcaaa tttttagcat aaaaaagtcc
     7801 attaattttg tacacaattc tgaaacttaa aaatctaaac tttcatcaat ttttttcata
     7861 atttcaataa attaacctta attttaagat atattctgaa atttggtttg aaagttgttt
     7921 ttacattata tttcaatact ttaaatcaaa aaattggatt ttttttgaaa aactcaatga
     7981 aagtttattt tttatttaaa aacaac
//
