LOCUS       NZ_CP007612            12003 bp    DNA     circular CON 08-APR-2024
DEFINITION  Bacillus thuringiensis serovar kurstaki str. YBT-1520 plasmid
            pBMB11, complete sequence.
ACCESSION   NZ_CP007612
VERSION     NZ_CP007612.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02797740
            Assembly: GCF_000747545.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis serovar kurstaki str. YBT-1520
  ORGANISM  Bacillus thuringiensis serovar kurstaki str. YBT-1520
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 12003)
  AUTHORS   Chen,B., Fu,Y., Zhang,S., Li,X., Qiang,H., Li,M., Zheng,H., Sun,M.,
            Yu,Z. and He,J.
  TITLE     The Complete Genome Sequence of Bacillus thuringiensis subsp.
            kurstaki Strain YBT-1520
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12003)
  AUTHORS   Chen,B., Fu,Y., Zhang,S., Li,X., Qiang,H., Li,M., Zheng,H., Sun,M.,
            Yu,Z. and He,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) State Key Laboratory of Agricultural
            Microbiology, Huazhong Agricultural University, No. 1 Shizishan
            Street, Wuhan, Hubei 430070, P. R. China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: PacBio v. 2.0
            Reference-guided Assembly         :: CP004069.1
            Genome Coverage                   :: 150x
            Sequencing Technology             :: 454; Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000747545.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 03:11:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,672
            CDSs (total)                      :: 6,517
            Genes (coding)                    :: 6,079
            CDSs (with protein)               :: 6,079
            Genes (RNA)                       :: 155
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 108
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 438
            CDSs (without protein)            :: 438
            Pseudo Genes (ambiguous residues) :: 0 of 438
            Pseudo Genes (frameshifted)       :: 211 of 438
            Pseudo Genes (incomplete)         :: 245 of 438
            Pseudo Genes (internal stop)      :: 99 of 438
            Pseudo Genes (multiple problems)  :: 93 of 438
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP007612.1.
            Source bacteria is available from Jin He(hejin@mail.hzau.edu.cn)
            and Ziniu Yu(yz41@mail.hzau.edu.cn).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12003
                     /organism="Bacillus thuringiensis serovar kurstaki str.
                     YBT-1520"
                     /mol_type="genomic DNA"
                     /strain="YBT-1520"
                     /serovar="kurstaki"
                     /db_xref="taxon:570416"
                     /plasmid="pBMB11"
     gene            join(11519..12003,1..10)
                     /locus_tag="DF16_RS36595"
     CDS             join(11519..12003,1..10)
                     /locus_tag="DF16_RS36595"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_230655829.1"
                     /translation="MNALIDTIFNPVLGWLAQMAKYLSDLSVPASRPLRISDYLGVFAY
                     LYGWTTVITTLFALVFVYFVCYIIMTYSSLFIRFKDTVNTIFNPVLGWLAQMAKYLSDL
                     SVPASRPLRISDYLGVFAYLYGWTTVITTLFALVFVYFVCYIIMTYSSLFIRFKDTVKW
                     W"
                     /name="hypothetical protein"
     gene            431..793
                     /locus_tag="DF16_RS29420"
                     /old_locus_tag="DF16_pBMB11orf00002"
     CDS             431..793
                     /locus_tag="DF16_RS29420"
                     /old_locus_tag="DF16_pBMB11orf00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000880242.1"
                     /translation="MLAKTKRKVQPQFEGHRALIIKEEGTCVVEQVISFDSEILETSTA
                     MLPRENELRLLDDNHGYVYHIYNLEKPARIEAENLKMLRRSSALKGIFDFDTGKKPFDI
                     IGFMPWVIIVLLAIFK"
                     /name="hypothetical protein"
     gene            807..1169
                     /locus_tag="DF16_RS29425"
                     /old_locus_tag="DF16_pBMB11orf00003"
     CDS             807..1169
                     /locus_tag="DF16_RS29425"
                     /old_locus_tag="DF16_pBMB11orf00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000116007.1"
                     /translation="MSVQEMLTKDILEEEGSNETIETVQLFQFMKKHAVPLTKKQQMAM
                     LLLNENGLSDISLYIAGMKEEVLPLSEYHKTLEKLTLSDRIKGNAKLSHLLKANANPSL
                     SLSANSAKVVKGNELA"
                     /name="hypothetical protein"
     gene            1166..1768
                     /locus_tag="DF16_RS29430"
                     /old_locus_tag="DF16_pBMB11orf00004"
     CDS             1166..1768
                     /locus_tag="DF16_RS29430"
                     /old_locus_tag="DF16_pBMB11orf00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024420.1"
                     /translation="MNIMVFEGFLGSGKTFGMSLFAKHYEEKSGCVLYSNYGLIGSKPF
                     VTLDTFHDIAKEKSSILNLDEAHIDLDARSFSSNSVKFFSQLSYYLRKLRCTLFITSPS
                     FDDLDSRIRGITNVLVRVSNDKNYFYYKMYDVQSKRYLKTMRIQKKKAFAIGSKVYDTT
                     AMVSPVQVPDKRQDFMEFLEALKSTAEEYGRQYKHSA"
                     /name="hypothetical protein"
     gene            1839..2030
                     /locus_tag="DF16_RS29435"
                     /old_locus_tag="DF16_pBMB11orf00005"
     CDS             1839..2030
                     /locus_tag="DF16_RS29435"
                     /old_locus_tag="DF16_pBMB11orf00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000511841.1"
                     /translation="MGILKKKKFREEVKRINKAHGEMREFLDLLMDRYGLDEEEVKNCE
                     VIKHHFDNLDLMFSQMAK"
                     /name="hypothetical protein"
     gene            2648..2854
                     /locus_tag="DF16_RS29440"
                     /old_locus_tag="DF16_pBMB11orf00007"
     CDS             2648..2854
                     /locus_tag="DF16_RS29440"
                     /old_locus_tag="DF16_pBMB11orf00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000365510.1"
                     /translation="MDLPVCNEKEVTVLSKTLKYLRSDQSNQVKVHKEYLEDRGWIFDD
                     LTYIDSNIYVVYKKEVVKRNKAF"
                     /name="hypothetical protein"
     gene            2866..3285
                     /locus_tag="DF16_RS29445"
                     /old_locus_tag="DF16_pBMB11orf00008"
     CDS             2866..3285
                     /locus_tag="DF16_RS29445"
                     /old_locus_tag="DF16_pBMB11orf00008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000473093.1"
                     /translation="MFKKFYREYIHVLFNIVAMFFYFSWCLVGAEFNLLTLIAIGIALF
                     IFVKYVSMDKKFVNENIDKLKDKKMIFRKYAISGEDIGYAVFTNFLLFILYLGISVYFK
                     PLSVMALLFAFIVNVSFLMLTLRIMSRLSLKNSKV"
                     /name="hypothetical protein"
     gene            3297..3632
                     /locus_tag="DF16_RS29450"
                     /old_locus_tag="DF16_pBMB11orf00009"
     CDS             3297..3632
                     /locus_tag="DF16_RS29450"
                     /old_locus_tag="DF16_pBMB11orf00009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000457469.1"
                     /translation="MFAKYYSQDDEFMSPLQFIALGGIMSFLIKFGCISPSVNMLTLFV
                     TFVTCIGLCYFFLLLITKYFYRFLAKHRDNLHVYLCNKYNEKIANRIGLCIGLVYIVGV
                     LVICLLI"
                     /name="hypothetical protein"
     gene            3931..4143
                     /locus_tag="DF16_RS29460"
                     /old_locus_tag="DF16_pBMB11orf00011"
     CDS             3931..4143
                     /locus_tag="DF16_RS29460"
                     /old_locus_tag="DF16_pBMB11orf00011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000216293.1"
                     /translation="MTVKEKKKTWAFTFTESEIQIIDEIVDIENDRRHSIAREHNLPFK
                     KYNRSTFVLAMIEEKKRKYQEQGEI"
                     /name="hypothetical protein"
     gene            4346..4567
                     /locus_tag="DF16_RS29465"
                     /old_locus_tag="DF16_pBMB11orf00012"
     CDS             4346..4567
                     /locus_tag="DF16_RS29465"
                     /old_locus_tag="DF16_pBMB11orf00012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000391857.1"
                     /translation="MEELLHKIIDKLDKIDKDIQEIKTDIKAHRIETDDNFIKLYHLVE
                     EKDIEIDVLNKRLFKNEATVERIKREKH"
                     /name="hypothetical protein"
     gene            4581..4859
                     /locus_tag="DF16_RS29470"
                     /old_locus_tag="DF16_pBMB11orf00013"
     CDS             4581..4859
                     /locus_tag="DF16_RS29470"
                     /old_locus_tag="DF16_pBMB11orf00013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000331888.1"
                     /translation="MAYSYCVDCERVYNCEDEKCCCGSKKRSVDWEEFAFIMGYPLYPV
                     KKKYGVFHLDLSKRLDADALCDLKADNPELFYYEFKGWKERELKKRN"
                     /name="hypothetical protein"
     gene            5186..5377
                     /locus_tag="DF16_RS29475"
                     /old_locus_tag="DF16_pBMB11orf00015"
     CDS             5186..5377
                     /locus_tag="DF16_RS29475"
                     /old_locus_tag="DF16_pBMB11orf00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000048809.1"
                     /translation="MSKVKGIRLNLRVSEDEYNKLQYVKECLLMEGLDLKDSAVIRYAL
                     LQTYKDYKEMNDLLKSRN"
                     /name="hypothetical protein"
     gene            5564..6634
                     /locus_tag="DF16_RS29480"
                     /old_locus_tag="DF16_pBMB11orf00016"
     CDS             5564..6634
                     /locus_tag="DF16_RS29480"
                     /old_locus_tag="DF16_pBMB11orf00016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410863.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_000410863.1"
                     /translation="MEKHIEKLSLSVCVDWLEFTFVYGEEFESICSFLGLDPTVFSKEI
                     DGFHKSYGYLSRYSFEEIHILMHGADNRSRIIMSGSGCRWFETLSSVGWYGLFDRIAFA
                     DEHGFSWIKVDRLDIAIDDFVGYFSVKKLKSKIKRRECLSRWKTSFVVETFDLEQGRLQ
                     GSTIYFGSASSKLRWVVYDKLEEQKKKNKDSDSIQGLEFWTRHELRLKKERADRAVKQL
                     VVNDFSIEEMYFGIINNYISFRVVDKEDSNRNRWNIAPFWRQYIGQASKLRLAEALPVA
                     NVERTFKWLSHSVSKKLYLLSEIFSQDDDTKKEFASYLENKGKQKITKGDLDLIENFPI
                     RDFKEFLKIYNEKCLK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            6795..7100
                     /locus_tag="DF16_RS29485"
                     /old_locus_tag="DF16_pBMB11orf00017"
     CDS             6795..7100
                     /locus_tag="DF16_RS29485"
                     /old_locus_tag="DF16_pBMB11orf00017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001176491.1"
                     /translation="MQLMKVMLTGCKKVDFTNDNNERIEGVNIHFLTTTDNSEQSGFGY
                     LPSKVFFRKEFIEKFSSLNYPIVAEAIIAFNMSPRGIQTKISDFNILEEFPFTALK"
                     /name="hypothetical protein"
     gene            7694..7846
                     /locus_tag="DF16_RS35570"
                     /old_locus_tag="DF16_pBMB11orf00021"
     CDS             7694..7846
                     /locus_tag="DF16_RS35570"
                     /old_locus_tag="DF16_pBMB11orf00021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001113828.1"
                     /translation="MPEFGFRPSMSQLFGNFHMNWSTMMPLLVILFGILFGFFVAHLVK
                     DKFDD"
                     /name="hypothetical protein"
     gene            7867..8025
                     /locus_tag="DF16_RS35575"
                     /old_locus_tag="DF16_pBMB11orf00022"
     CDS             7867..8025
                     /locus_tag="DF16_RS35575"
                     /old_locus_tag="DF16_pBMB11orf00022"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425806.1"
                     /translation="MEMPKITLTYNLADVANGLTNWLGAYWPIIAFSVAIPLTFLVAFN
                     TKDLFTR"
                     /name="hypothetical protein"
     gene            8132..9538
                     /locus_tag="DF16_RS29490"
                     /old_locus_tag="DF16_pBMB11orf00023"
     CDS             8132..9538
                     /locus_tag="DF16_RS29490"
                     /old_locus_tag="DF16_pBMB11orf00023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015386301.1"
                     /translation="MKKLAIIFLCAFCIVSHTIPVFADSPSFQLVGSKNVTIENNGIVN
                     GKYNLIIKVNNMKDVYSFQLSELMLVNDCSFHVGKSKWTRPEQEKNEFPINWNQNGTYA
                     FYIFHNGKVLGYARFKLKGFKENGGGGGETVYWGKGAKYYDLPEIEKPNPEDYADYDNK
                     NDGVCTNEKIPDDKPQGSGDIDESKEDNKPPVDGGGGSDKAVKEVLEKIVSAVNEISKN
                     TGETAKNTGETAKNTGEIKKNTDEIAKNTKEIADAVKDIRDNLKIPDDMDLSIKPVLPE
                     DIKPDKPKQPDKPFEDKQEHFKEGEQEKPPDKLPVAPEPAECWGDVCKDKDNQKEEDMK
                     KEKDMDKEEPMKPDKPMEKEDMKPDKPMTPDKPMEKEDMKPDKPMTPDKPMEKEDMKPD
                     KPMTPDKPMEKEDMKPDKPMTPDKPMDREGEMKKDNDMKQDKPMTPWEEMKPSDEGSEL
                     CWHVVNGKCG"
                     /name="hypothetical protein"
     gene            9505..10932
                     /locus_tag="DF16_RS29495"
                     /old_locus_tag="DF16_pBMB11orf00024"
     CDS             9505..10932
                     /locus_tag="DF16_RS29495"
                     /old_locus_tag="DF16_pBMB11orf00024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016090439.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="WP_228176732.1"
                     /translation="MLACSEWKMRIKKFFIVVPVLLGILFVGDISAFAQGKDVLSGRVG
                     KAQYGSSYITSLTDGNLQTSDTLSNGEVIIDLASPVRVSGFRFTKVGGSSNRMDVYFYT
                     EGATLKREYRNTDSGYLQAIDIKGVKKIGLKNMSNGDYFTLAEVNLYEYESVPPSDITD
                     LKENIKHDSVEFTYKNPKSNFSHLRIYRDGQVLASDVKEEKFTDKGLTPETEHIYKFVS
                     VSPDGLESKGIEKKIKTAKEPLPDEIKDLQEDEITHTYVKFSYKFPKNNFDHVKIYRDD
                     KVIADNVKVEEFVDKGLKGSTVYTYKFVSVSSTGLESNGIVKKVQTDEEPNLNKPSKPS
                     NFDITPKDGSLVVNLLSQNLGVPIKGYHIFIDGVKVNDSLVTSRSYAVKGLQNGQTYGV
                     QIKAVSKWNIESDLSNVVNGIPKVPVIPEVTFSFGLGALVDGITNWFLGIWPIVAFSIA
                     IPLAFIVAFNTKKLFMR"
                     /name="fibronectin type III domain-containing protein"
     gene            10942..11097
                     /locus_tag="DF16_RS35580"
     CDS             10942..11097
                     /locus_tag="DF16_RS35580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015386302.1"
                     /translation="MIQPVTTKDLIQFLQYAFHAGEDVLFLAFVLMITFSVAVSIRRIM
                     VGYKQS"
                     /name="hypothetical protein"
     gene            11094..11519
                     /locus_tag="DF16_RS29500"
                     /old_locus_tag="DF16_pBMB11orf00025"
     CDS             11094..11519
                     /locus_tag="DF16_RS29500"
                     /old_locus_tag="DF16_pBMB11orf00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000970219.1"
                     /translation="MMEKFVKFLKGGFEAVIKSLKTFIDFLATPFQYLLDFLKGIFYFI
                     EKLFQVVVAIIKLFVALFQFLGAVIAGFFKTLFLWVQPNFDGGNVSFPSASMKGFKVAT
                     DILLPTGLLTVIPAVVTCFIWYLFVKKLMDLFRGGHH"
                     /name="hypothetical protein"
     Typing          5565..6634
                     /name="rep_cluster_814"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5565..6631
                     /name="MOBT"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggtggtaa tgatattgaa tattgtagga caagcaattt tattaatatt ttttacgaca
       61 gttgtttctt ggatggtaat agatacattg aatttcttta gaggtataga agaatgattt
      121 atgcaacgtt ggggtttatt agttggattt tgattttgat ttgtgaagta acatcgagaa
      181 aaggagattt aaaataatgc aggcttttaa tgcgatgttt gagttatcta gtgcggtttg
      241 tttatctgta tttcttttta tagttttacc atatatgttt ttcaaaaata aaatgtcaaa
      301 gtgataaaaa cgaatgttaa atatcaatta gtttatatag ttcgttaaaa agattcaaaa
      361 tgtcatggca gcgggactag atccaagaga ttttgaaatg aaaaagcgaa cgttttggag
      421 ggttttataa atgttagcta aaacaaaacg gaaagtacag ccacaatttg aagggcatag
      481 agcattgatt attaaagaag aaggaacatg tgtagttgaa caggttatat cgtttgattc
      541 ggagatttta gaaacgagta cagccatgtt accacgtgag aatgaattga gattacttga
      601 tgataatcat ggttatgtgt atcacattta caacttagaa aagccagcaa gaattgaagc
      661 ggaaaatttg aaaatgttac gtcgttctag tgcattaaag ggaatttttg attttgatac
      721 agggaagaaa ccttttgaca ttattggttt tatgccttgg gtaattattg tattgctagc
      781 tatttttaaa taatggggtg gagtaaatga gtgttcagga aatgttaacg aaagatattt
      841 tagaagaaga aggttcaaac gaaacgattg agactgttca actgtttcaa ttcatgaaaa
      901 aacatgctgt gccattaaca aagaaacaac aaatggctat gttattgctt aatgaaaatg
      961 gattatcgga catttcttta tatattgctg gaatgaaaga agaagtattg ccattgagtg
     1021 aataccacaa gacacttgaa aaattaacgc tttctgaccg aattaaaggt aatgctaaat
     1081 taagtcattt actgaaagca aatgcgaatc cttcattatc attgtcagca aatagcgcaa
     1141 aagttgttaa agggaatgag ttagcatgaa cataatggtt tttgagggct ttctcggtag
     1201 cggtaaaacg tttggtatga gtttgtttgc aaaacactat gaagaaaaga gtggctgtgt
     1261 attgtacagt aattacggtt taattgggtc taaaccattt gtaactttag atacgtttca
     1321 tgatattgca aaagagaaat ctagtatctt aaatctcgat gaagcgcata ttgatcttga
     1381 tgctagaagt ttttcaagta actcggtaaa gttcttttct caattgtctt attaccttag
     1441 aaaactacgt tgtacgttgt ttattacctc accatcattt gatgatttag attcacgtat
     1501 aagaggtatt acaaacgttc tcgttagggt atcgaatgat aaaaactatt tttattacaa
     1561 aatgtatgat gttcaatcta aacgatattt aaaaacaatg cgtattcaaa agaagaaagc
     1621 ttttgctatt ggttcaaaag tttatgatac gacagcaatg gtttctccag tacaagttcc
     1681 agataaacga caagacttca tggaattttt agaagcgttg aagtcgaccg ccgaagagta
     1741 cggacgccag tacaagcatt cagcgtaagc tgtatgcgcg gtgcaggcgg gaggacttca
     1801 taagattata taaattaaat aaggaaggta cgttatagat gggaatttta aagaagaaaa
     1861 aatttagaga agaagtgaaa agaataaata aagcacatgg agaaatgaga gaatttctag
     1921 atttgctcat ggatcgctac ggattagatg aagaagaagt aaaaaattgt gaagtaatta
     1981 aacatcattt tgataattta gatttgatgt ttagtcaaat ggcaaaataa tgttcgtgat
     2041 ttaggtgtga aatgtaacgg cagccgtgca tattttccag gggcttttaa tgaaaatacg
     2101 aacattaagt atgaggtgaa gagatttgat acatgtagat tggttgaaaa ctatggcgag
     2161 agacggatta ttttatggtt cattcattgc tgtaattgtt ttcttgctaa attggtttac
     2221 gggttaggtt ttaaaggtct tccttcgctt ttgaggttgg agattggctg gggtgtgggg
     2281 ctagccccgc atagcgtaat gggtaaatag aataaaagaa aatataaagg tcggtagtac
     2341 aagcgtttag gctcgtataa tggataggca agttgatagt taaatatatt cacttttgag
     2401 tttatcgact tggcgatgaa gacaaacgag cctaaaaaag ttatgcaaga atgtagcgta
     2461 gcggaaaagg tggagatttt atgaagcaga gcgtaagaaa atactacctg tttcttgttt
     2521 aaccttttag caaatacaga tagagggttg ttttgatgga gaatgttatt gttacgttgt
     2581 ttggtactct agggatttgt ttcttgttta aaggcatgct agatacttta aaagaagggt
     2641 gatatttgtg gatttgcctg tttgtaatga aaaagaggta actgttttat ctaagacatt
     2701 gaaatatttg agaagtgatc aatctaatca agttaaggta cataaagagt atttagagga
     2761 cagaggttgg atttttgatg atttaactta tatagatagc aatatctatg tagtttataa
     2821 aaaagaggtt gtaaagcgaa ataaggcgtt ttaaggggtg tatatatgtt taaaaagttt
     2881 tatcgtgaat atatccatgt attgtttaat attgttgcga tgttttttta ttttagttgg
     2941 tgtttagttg gtgctgaatt taatttatta actctaatag caattggaat tgcccttttt
     3001 atttttgtta agtatgtatc tatggataag aaatttgtta atgaaaatat agataaatta
     3061 aaagacaaaa agatgatttt taggaaatat gctataagtg gtgaagatat tggatatgca
     3121 gtttttacaa attttttatt gtttattttg tatttaggaa tatccgtata ttttaagcct
     3181 ttaagtgtaa tggctttatt atttgctttt attgttaatg ttagttttct tatgttaact
     3241 cttagaataa tgagtagatt aagtttaaag aatagtaaag tttaagaggt gtatatatgt
     3301 ttgctaagta ttatagccaa gatgatgaat ttatgtcgcc acttcaattt atagctttgg
     3361 gtggtattat gagttttctt attaaatttg gttgtatttc gcctagtgtg aatatgttaa
     3421 cgttatttgt tactttcgtt acttgtattg gattatgtta tttttttcta cttttaataa
     3481 caaagtattt ttatagattt cttgctaaac atcgtgataa tttacatgtt tatttatgta
     3541 ataagtacaa tgaaaaaata gcaaatagaa tcggtttatg tattggttta gtttatattg
     3601 taggggtgtt agttatatgt ttgctgattt aaagttgaat tttaaatttt ggttaactgt
     3661 agtttttgat atatgtatgc ttgcaatgat ctgtgttgtt acaacaaagt atgtatctga
     3721 tatatatagt gatttgttta ttttcacgac aggattttta atactattaa tttggtattc
     3781 tgttagagtg tatttgatag atttaatcag aaaaaaacct atcaataatt aaaatttaat
     3841 atttttttat tgaaaaacca tatgttttta tttacaaacc atatggtttt tgttatatta
     3901 aatttagtta attgatagga ggttgttttt atgacagtta aagaaaagaa aaaaacttgg
     3961 gcatttacat ttactgaaag tgaaattcag ataatagatg aaattgtaga tatagagaat
     4021 gatagaagac attcaattgc tagagaacat aatttaccgt ttaaaaaata taatcgttct
     4081 acatttgtgc ttgcaatgat tgaagaaaag aaacgtaagt atcaagagca aggagaaatt
     4141 taatggaaat tgttttaggt gttagtgcgt ttggtttgta tatttcgttt ttaatatttt
     4201 tatattggat ggcattagat tttttagctt ggttatataa aaagatttaa taaaatcgtt
     4261 attttattgg ttttctctta acttgttaat attaataggg gatagagtct aactttttta
     4321 aaattatatt ttggaggtcg taactatgga agaattgtta cataagatta ttgataaatt
     4381 agacaagatt gataaggata ttcaggagat taaaactgat attaaggctc accgaattga
     4441 aactgatgat aattttatta agctatatca tttagttgaa gaaaaagata ttgagattga
     4501 tgtgttgaat aagcgtttgt ttaagaacga ggcgacagtt gaacgtatta agagggaaaa
     4561 gcattaggag gaaattgtcg atggcatata gttattgtgt ggattgtgag cgtgtgtata
     4621 actgtgaaga tgaaaagtgt tgttgtggaa gtaaaaaacg ttctgttgat tgggaagagt
     4681 ttgcttttat tatgggttat ccgttatatc ctgttaagaa aaaatacggt gtttttcatt
     4741 tagatttatc taaacgttta gatgctgatg ctctatgtga tcttaaagca gataatccag
     4801 aactttttta ttatgagttt aaaggttgga aagaaagaga attgaaaaag agaaattaat
     4861 aataaatatt ttggagttag tgggtgtttt gtttgtctaa ttcgaaggaa aatttccagt
     4921 tgcgaaatga ggaacccccg tccagtaaca cgggggttcc cggctgtttt gatatctaca
     4981 ctatcccttg cctagactgg gttcaagtca cttttaaatc tgtacagaat tcacaaaaaa
     5041 atgcacggaa aattattagt aaaatttcca gtttttaatg aagcttaaaa gattcttata
     5101 ttggtataat atttacaagg aggaaaatgg aaactcttta catatggtgt atgacattgt
     5161 atgcttcaat tgaggaggtt atataatgtc aaaagttaaa ggtattcgtt tgaatttaag
     5221 agtttctgaa gatgagtata acaagttgca gtatgttaaa gaatgtttac ttatggaagg
     5281 tcttgatctt aaagattcag ctgttattcg ttatgcttta ttacagactt ataaggatta
     5341 taaagaaatg aatgatttgt tgaaatcaag gaattgaata gctgatttca tgtttctact
     5401 gggggctagt aacaccccag tagaaaatac aggtcaatca cctccgaacg cttgtggctc
     5461 tagggttcgt ccttctttgt ttttttcacg gttcttttaa aaaataggca aaaaattcgc
     5521 ggtttttatt ttgcttttat tgtattgttt tagaggtgtt tgtatggaaa aacatattga
     5581 aaaattgtct ctttcggttt gtgtggactg gttggagttt acttttgttt atggtgaaga
     5641 gtttgaatct atttgtagtt ttttaggtct agatcctact gttttttcta aggaaataga
     5701 tggttttcac aagtcttatg gttatttgtc tagatattct tttgaagaaa tacacattct
     5761 tatgcatggt gctgataatc gttctagaat tattatgtct ggttctggtt gcagatggtt
     5821 tgaaacatta tcctctgttg gctggtatgg tttgtttgat aggattgcat ttgcagacga
     5881 acatggtttt tcttggatta aagttgaccg tttagatatt gctattgatg attttgtagg
     5941 ttatttttct gttaaaaaat taaagtctaa aataaaaaga agagaatgtt tgagtcgttg
     6001 gaaaacaagt tttgttgttg aaacttttga tttagaacag ggacgattgc agggttctac
     6061 gatttatttt ggaagtgctt ctagtaaatt gagatgggtt gtttatgata agttagaaga
     6121 gcagaaaaaa aagaataaag atagtgattc gattcaaggt ttagaatttt ggactaggca
     6181 tgagttgcgt ttgaaaaagg agcgtgctga tcgtgctgtt aaacaacttg tagttaatga
     6241 ttttagtatt gaagaaatgt attttggtat tataaataat tatatttcgt ttcgtgttgt
     6301 cgataaggaa gattctaata gaaataggtg gaatattgca cctttttgga ggcaatatat
     6361 aggacaagca agtaaattaa gattagcaga ggctttacct gttgcaaatg ttgagcgtac
     6421 gtttaaatgg ttgtctcata gtgtttctaa aaaattatat ttgttgtctg aaattttttc
     6481 gcaagatgat gatacaaaga aagagtttgc tagttattta gaaaataagg gcaaacaaaa
     6541 aatcacgaaa ggggatttag acttaataga aaatttccca attcgtgact tcaaagaatt
     6601 tttaaagatt tataatgaaa aatgtctaaa ataattttac tctattttat aatggaagtc
     6661 taatacatta aaaaatccat atatttttaa aaaaagacta gaattttttt ctagtttttc
     6721 ttataattaa atgtatgaca aaggtgtaca tagaacgaca ttgtatgact aaaaagattc
     6781 taaggagtgt aaatatgcaa ttaatgaaag taatgttaac aggttgtaag aaagttgatt
     6841 tcacaaatga taataatgaa agaatcgaag gtgttaatat tcacttttta actacaactg
     6901 ataatagtga gcaatctggt tttggatatc ttccttctaa ggtctttttt agaaaagagt
     6961 ttatagaaaa atttagttct ttgaattatc caattgttgc tgaagctatt attgctttta
     7021 atatgtctcc tcgtggtatt caaactaaaa tttctgattt taatatttta gaggaatttc
     7081 cttttacagc tttaaagtga ttttatgata gataaaatcg atttaaatga attgtacaaa
     7141 actatttcgg aaattatgcc taattactta gtgcagacta agaatggtag ttttgcagtt
     7201 ttccagacac ttacctatgg tgatatgtta attagtttct tgttattgct aatcttgttg
     7261 gtgttgacat tcaactggat atggagtgct ttacgatgat cgagtttaat ttagaaaact
     7321 tgttgaatca atattccttg tttggattac catttttatt aattatcgtg ttcacattgc
     7381 tgaaagattg gcggtttgat agataatgaa tgatttagcg tttctagata tgttgtattg
     7441 gttcttcatg aacaattcag ttgttttata ctcgttaggt tttataacct ttctagtatt
     7501 cgtaataatg atgttcacca ccgtcaaagc ttggggtgat ggctattagt tttagcatgg
     7561 acgtgggaag aatcttacag tatgccttta acatgtttag ttcggtgtta ccagtgattt
     7621 atttattcat tggtggggcg tttgctatct actgtatcgg taaagtgtac aacaccttta
     7681 ggagttgatt tcaatgcctg aatttggatt cagaccatcg atgtctcagt tattcggtaa
     7741 ctttcatatg aactggtcaa caatgatgcc attgttagtc attttgtttg ggatactgtt
     7801 tggatttttc gtagcgcatt tagtaaaaga caaatttgat gattgattaa ttaggaggaa
     7861 tttattttgg aaatgccaaa aattacattg acttataatc ttgcagacgt tgctaacggt
     7921 ttgactaact ggttaggagc atattggcct attatcgcat tctcggttgc gattccatta
     7981 acgttcttag tggcgtttaa cactaaagac ctatttactc gttaatcatc aaataatcac
     8041 tccttaaata gcaagaagtt aactataaat ttaataaaac aattaaatag ctgggctaat
     8101 aacctagcta tttttgttag gaaggattga tatgaaaaag ctagccataa tttttttatg
     8161 tgcattttgt attgttagcc atacgatccc tgtgtttgct gattcaccta gttttcaatt
     8221 ggtaggtagt aaaaatgtaa caatcgaaaa taatgggata gttaatggta aatacaattt
     8281 aattattaaa gttaacaata tgaaagatgt atatagtttt cagttatcgg aattgatgct
     8341 tgttaatgat tgctcgttcc atgttggtaa atcaaagtgg actcgtccag aacaagaaaa
     8401 aaacgagttc cctattaact ggaatcagaa tggtacatac gcattttaca tttttcataa
     8461 tggtaaagtt ttaggctatg cacgttttaa gcttaaagga tttaaagaaa acggtggcgg
     8521 tggaggagaa actgtatatt ggggcaaagg tgctaaatac tacgatttac ctgaaattga
     8581 aaagcctaat cctgaggact atgcggatta tgataataag aatgacggag tttgtacaaa
     8641 tgaaaaaatt ccagatgata aaccacaagg cagcggtgat atagatgaat caaaagaaga
     8701 taataaacca cctgtagatg gtggcggtgg ttctgataaa gctgttaaag aagttttaga
     8761 aaagattgtt agtgcagtaa atgaaatttc aaagaataca ggtgaaacgg ctaaaaatac
     8821 aggtgagaca gcaaagaata caggtgagat taaaaagaac accgatgaaa tagcaaagaa
     8881 cacaaaagaa attgcggatg ctgttaagga tattagggat aatttgaaga ttcctgacga
     8941 tatggatttg tcgattaaac ctgttctacc tgaggacatt aagcctgata aacctaaaca
     9001 acctgataag ccttttgaag acaaacaaga gcactttaaa gagggtgaac aagagaaacc
     9061 acctgacaaa cttcctgttg ctcctgagcc cgctgaatgt tggggggatg tttgtaaaga
     9121 taaagataat cagaaagaag aagatatgaa gaaagaaaaa gatatggata aagaagaacc
     9181 aatgaagcct gataaaccaa tggaaaagga agacatgaaa ccagataagc caatgactcc
     9241 tgataagcca atggaaaagg aagacatgaa gcctgataag ccaatgactc ctgataagcc
     9301 aatggaaaag gaagacatga agcctgataa accaatgact cctgataaac caatggaaaa
     9361 ggaagacatg aaacctgata aaccaatgac tcctgataag cctatggatc gggagggaga
     9421 aatgaaaaaa gataatgata tgaaacaaga taagccaatg actccttggg aagaaatgaa
     9481 accaagtgac gaggggagtg aactttgttg gcatgtagtg aatggaaaat gcggataaag
     9541 aaatttttta tagtagtacc tgtattactt gggattttgt ttgtaggtga tataagtgca
     9601 tttgctcaag gtaaagatgt attaagtgga agagtaggta aagctcaata tggtagttca
     9661 tatataactt cactaactga tggaaattta caaactagtg atactctttc taatggtgag
     9721 gtcataattg atttagcttc tcctgttaga gtttcagggt ttaggtttac taaagttggt
     9781 ggctctagta ataggatgga tgtttatttt tatacagagg gtgctacttt aaagcgggaa
     9841 tatagaaata ctgatagtgg ttatctacaa gccattgata ttaaaggtgt taaaaaaatt
     9901 ggattgaaaa atatgagcaa tggagattat tttacacttg ctgaagttaa tttatatgag
     9961 tatgaatctg ttcctccatc tgatattacg gatttaaaag aaaatattaa acatgattca
    10021 gttgaattta cttataaaaa tccgaagtcg aattttagtc atttaagaat ttatcgtgat
    10081 ggacaagtgc ttgctagtga tgtgaaagaa gaaaaattca ctgataaagg tttaactcct
    10141 gaaacggagc atatttataa atttgtttca gtttctcctg acggtcttga atcaaaaggt
    10201 atagagaaaa agataaagac agcgaaagag cctttaccag atgaaataaa agatttacag
    10261 gaagatgaaa taactcatac ttatgtgaaa tttagttata agtttcctaa aaataatttt
    10321 gatcatgtga aaatttatcg tgatgataag gtgattgcgg ataatgtgaa ggttgaggaa
    10381 tttgttgata aaggtctaaa aggaagtact gtatatactt ataaatttgt ttcagtttcc
    10441 tctactggtc ttgaatcaaa tggaattgtg aagaaagtac agactgatga agaacctaat
    10501 ctcaataagc cttctaaacc ttcgaatttt gatattacgc caaaagatgg ctcgttagtt
    10561 gtaaatctat taagtcaaaa tttaggcgtt ccaattaagg gttatcacat ttttattgat
    10621 ggtgtaaaag ttaatgattc acttgttaca agtcgttctt atgctgttaa aggtttacag
    10681 aatggtcaaa cttacggtgt acagattaag gcggtttcta agtggaatat tgaaagtgat
    10741 ttaagcaatg ttgttaatgg aattcctaaa gtaccagtta ttcctgaggt tacctttagt
    10801 tttggtttag gtgctttagt tgatgggata acaaattggt tcctggggat ctggccgatt
    10861 gtggcattta gtattgctat tccattagcg tttattgttg cgtttaatac gaaaaagttg
    10921 tttatgaggt aggtgcttag tttgatacaa ccagttacaa ctaaggactt aatacaattt
    10981 ctccagtatg cgttccatgc tggggaagac gtattatttc tagcgtttgt attaatgatt
    11041 acattctctg ttgctgtaag cattagaagg attatggtgg gttataagca atcatgatgg
    11101 agaagtttgt taagttctta aaaggtggat ttgaagcggt tataaagtcg ttaaaaacgt
    11161 ttattgattt tttagctaca ccatttcaat atctactgga ttttttgaaa ggaatctttt
    11221 atttcataga aaagctgttt caggttgtag tagcaattat aaaattattc gttgccttgt
    11281 ttcagttttt aggtgctgta atagcgggat tctttaaaac tttatttcta tgggtacaac
    11341 ctaattttga tggtgggaat gtatcgtttc ctagtgcatc aatgaaaggt tttaaagtag
    11401 ctactgatat attacttccc actggattac ttacagttat tccggctgtg gtcacatgtt
    11461 ttatttggta cttgtttgtt aaaaagctca tggatctatt cagaggtggt catcattaat
    11521 gaatgcgtta attgatacaa tatttaatcc tgttcttggt tggcttgcac aaatggcaaa
    11581 gtatttaagt gatttaagcg taccggcaag ccgtcctttg cgaattagtg attaccttgg
    11641 agtatttgct tatttatacg gttggacaac cgttataacg acgttattcg ctttagtttt
    11701 cgtatatttt gtttgttaca tcatcatgac ttacagtagt ttatttattc gatttaaaga
    11761 tacagtgaat acaatattta atcctgttct tggttggctt gcacaaatgg caaagtattt
    11821 aagtgattta agcgtaccgg caagccgtcc tttgcgaatt agtgattacc ttggagtatt
    11881 tgcttattta tacggttgga caaccgttat aacgacgtta ttcgctttag ttttcgtata
    11941 ttttgtttgt tacatcatca tgacttacag tagtttattt attcgattta aagatacagt
    12001 gaa
//
